Rw5G009510

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000145
Physical Location & Seq
Forward (+)
10218831 .. 10221182
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G009510.1

Sequence Viewer

Length: 597 bp
ATGTCGGAAGATTACCCAAACGCCATTGTTCTGCCGCTCGAGGAGGCTGTGATGGTCCAAGATTACTTTGAAGTCGTCTCTTACACCAAGTTCTGCGATCGATTCCTTGACACAGATTTGCTGCAAAAAACTTGGCCCATAGTGGAGTCGTGTTTAAGCAAGCATGGTGTTCTGTGCACACTGGATCTGGCAATACGAACGCTGGATTGCAGTTACCAACATGAGATCATCAAGATTGGGAATCAAGAGGGGGGGATTTGCAACATATTTGGGATCAAGATGAAGCTTAATTTTCTGCCTTTTATTAATTGTCTGCAGGAGCAATTTCTTGCACGGAGGAATCTTCTCGCTGGTGTCATAAAGGGGCTTTTTGAACTGACTGGCTGTGGTGTTTTTCTTAAGGGAAATACCATTGCTCTTATTGGTCCACTGCAAGGAATAAAGACGATAACAAAGATTGTGGAAGACTGCATCGCTCATAATGTGCCTCCTGCACCTCGTGTGCGGAGGATTAAAAAGAAGACTGGATTGATGAAGGATGCGAGGATTAAAATGACGAGTGAAGTGATGATGAGTCTTGAGGCTTTTCATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.28

Weight (kDa)

8.09

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_2nd PF21800 106 - 177 2.6e-11 KRR1 small subunit processome component, second KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08420
fragaria_vesca FvH4_1g11160 FvH4_2g08143 FvH4_3g36450 FvH4_3g36450 FvH4_3g36450 FvH4_3g36450 FvH4_3g36460 FvH4_3g36460 FvH4_3g45301 FvH4_4g27690 FvH4_4g27690 FvH4_4g27690 FvH4_4g27690 FvH4_5g09160 FvH4_5g09161 FvH4_5g09380 FvH4_5g22900 FvH4_6g42520 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550 FvH4_6g42550
malus_domestica MD02G1015700.v1.1 MD02G1058900.v1.1 MD02G1059000.v1.1 MD03G1170900.v1.1 MD04G1231500.v1.1 MD13G1044300.v1.1 MD16G1045100.v1.1
prunus_persica Prupe.1G308200_v2.0.a1 Prupe.2G125300_v2.0.a1 Prupe.6G307800_v2.0.a1 Prupe.6G351700_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1 Prupe.6G351800_v2.0.a1
pyrus_communis pycom02g01420 pycom02g04810 pycom03g12790 pycom03g12800 pycom12g17840 pycom12g17850 pycom12g17860 pycom13g03890 pycom16g03970
rosa_chinensis RchiOBHm_Chr2g0090081 RchiOBHm_Chr3g0469061 RchiOBHm_Chr3g0469081 RchiOBHm_Chr4g0435531 RchiOBHm_Chr5g0014021 RchiOBHm_Chr5g0014031 RchiOBHm_Chr5g0014041 RchiOBHm_Chr7g0190151 RchiOBHm_Chr7g0190161 RchiOBHm_Chr7g0190171 RchiOBHm_Chr7g0210441
rosa_laevigata RLG00000003683 RLG00000003687 RLG00000003688 RLG00000004561 RLG00000004643 RLG00000016083 RLG00000021085 RLG00000024381 RLG00000032065 RLG00000032067 RLG00000032071 RLG00000032072 RLG00000032075
rosa_multiflora Rmu_co8284349.1_g000001 Rmu_co8324735.1_g000001 Rmu_co8355915.1_g000001 Rmu_co8388905.1_g000001 Rmu_sc0000302.1_g000041 Rmu_sc0000302.1_g000050 Rmu_sc0001940.1_g000034 Rmu_sc0003807.1_g000011 Rmu_sc0005803.1_g000001 Rmu_sc0005803.1_g000007 Rmu_sc0007139.1_g000007 Rmu_sc0007767.1_g000001 Rmu_sc0007767.1_g000003 Rmu_sc0007767.1_g000006 Rmu_sc0007933.1_g000005 Rmu_sc0008587.1_g000004 Rmu_sc0008968.1_g000004 Rmu_sc0018491.1_g000002 Rmu_sc0022420.1_g000001 Rmu_sc0027700.1_g000001 Rmu_sc0036218.1_g000001
rosa_roxburghii Rroxscaffold_1G00062590 Rroxscaffold_1G00062600 Rroxscaffold_1G00062620 Rroxscaffold_1G00062630 Rroxscaffold_1G00062640 Rroxscaffold_1G00062670 Rroxscaffold_1G00062680 Rroxscaffold_2G00091430 Rroxscaffold_2G00151410 Rroxscaffold_3G00245340 Rroxscaffold_3G00255390 Rroxscaffold_3G00264820 Rroxscaffold_3G00264830 Rroxscaffold_3G00265870 Rroxscaffold_5G00355140 Rroxscaffold_5G00376760 Rroxscaffold_6G00412230
rosa_rugosa Rorug02G0005200 Rorug02G0468300 Rorug02G0468300 Rorug02G0532400 Rorug03G0098900 Rorug03G0099000 Rorug03G0099400 Rorug04G0286000 Rorug05G0014400 Rorug05G0014500 Rorug05G0014600 Rorug05G0014600 Rorug05G0014600 Rorug05G0014800 Rorug05G0014900 Rorug05G0015000 Rorug05G0015100 Rorug05G0015100 Rorug05G0441300 Rorug06G0495300 Rorug06G0502600 Rorug06G0502700 Rorug07G0062300
rosa_samantha Rh2AG050900 Rh2BG049600 Rh2CG051800 Rh2CG234600 Rh2DG051100 Rh3BG171300 Rh3BG171800 Rh3CG336700 Rh3DG203600 Rh3DG203800 Rh4AG341600 Rh4AG341700 Rh4BG349900 Rh4CG364600 Rh4DG344200 Rh5AG108800 Rh5AG108900 Rh5AG109000 Rh5AG109100 Rh5BG105500 Rh5CG117300 Rh5CG117500 Rh5CG117600 Rh5DG104200 Rh5DG104400 Rh5DG104500 Rh5DG104600 Rh7BG102300 Rh7BG110600 Rh7BG110700 Rh7BG118900 Rh7CG103600 Rh7CG112800 Rh7CG113000 Rh7CG200300 Rh7CG271100 Rh7DG111300 Rh7DG111400 Rh7DG194400 Rh7DG261800 Rh7DG261900
rosa_wichuraiana Rw0G014550 Rw0G014580 Rw2G004580 Rw3G013980 Rw3G013990 Rw3G014000 Rw3G014020 Rw4G029810 Rw5G009500 Rw5G009510 Rw5G009520 Rw5G009530 Rw7G008640 Rw7G009350 Rw7G021680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 37
AciI CCGC 2 cut(s) 35, 505
AclWI GGATC 2 cut(s) 192, 281
AdeI CACNNNGTG 1 cut(s) 500
AfiI CCNNNNNNNGG 1 cut(s) 434
AflII CTTAAG 1 cut(s) 398
AgsI TTSAA 2 cut(s) 71, 374
AluBI AGCT 1 cut(s) 286
AluI AGCT 1 cut(s) 286
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 1 cut(s) 82
Alw44I GTGCAC 1 cut(s) 175
AlwI GGATC 2 cut(s) 192, 281
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 1 cut(s) 134
ApaLI GTGCAC 1 cut(s) 175
ApeKI GCWGC 1 cut(s) 121
AseI ATTAAT 1 cut(s) 306
AspS9I GGNCC 3 cut(s) 55, 135, 425
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 2 cut(s) 55, 425
BaeGI GKGCMC 1 cut(s) 179
BauI CACGAG 1 cut(s) 498
BbsI GAAGAC 2 cut(s) 471, 527
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 1 cut(s) 108
BccI CCATC 1 cut(s) 46
BcoDI GTCTC 1 cut(s) 82
BfmI CTRYAG 1 cut(s) 314
BfrI CTTAAG 1 cut(s) 398
BisI GCNGC 2 cut(s) 35, 122
BlsI GCNGC 2 cut(s) 36, 123
Bme18I GGWCC 2 cut(s) 55, 425
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 3 cut(s) 55, 135, 425
BmsI GCATC 2 cut(s) 480, 529
BpiI GAAGAC 2 cut(s) 471, 527
Bsa29I ATCGAT 1 cut(s) 100
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse1I ACTGG 3 cut(s) 186, 385, 529
Bse3DI GCAATG 1 cut(s) 411
BseCI ATCGAT 1 cut(s) 100
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 1 cut(s) 434
BseMI GCAATG 1 cut(s) 411
BseNI ACTGG 3 cut(s) 186, 385, 529
BseRI GAGGAG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 179
BseXI GCAGC 1 cut(s) 108
BsgI GTGCAG 1 cut(s) 477
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 1 cut(s) 136
BshVI ATCGAT 1 cut(s) 100
BsiEI CGRYCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 179
BsiHKCI CYCGRG 1 cut(s) 38
BslI CCNNNNNNNGG 1 cut(s) 434
BsmAI GTCTC 1 cut(s) 82
BsmBI CGTCTC 1 cut(s) 82
BsnI GGCC 1 cut(s) 136
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 4 cut(s) 97, 184, 225, 273
BspACI CCGC 2 cut(s) 35, 505
BspANI GGCC 1 cut(s) 136
BspDI ATCGAT 1 cut(s) 100
BspMAI CTGCAG 1 cut(s) 318
BspPI GGATC 2 cut(s) 192, 281
BspTI CTTAAG 1 cut(s) 398
BsrBI CCGCTC 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 3 cut(s) 186, 385, 529
BssMI GATC 4 cut(s) 97, 184, 225, 273
BssSI CACGAG 1 cut(s) 498
Bst2BI CACGAG 1 cut(s) 498
BstAFI CTTAAG 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 161
BstF5I GGATG 1 cut(s) 544
BstKTI GATC 4 cut(s) 100, 187, 228, 276
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 4 cut(s) 97, 184, 225, 273
BstMCI CGRYCG 1 cut(s) 100
BstSFI CTRYAG 1 cut(s) 314
BstSLI GKGCMC 1 cut(s) 179
BstV1I GCAGC 1 cut(s) 108
BstV2I GAAGAC 2 cut(s) 471, 527
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 100
BsuRI GGCC 1 cut(s) 136
BsuTUI ATCGAT 1 cut(s) 100
BtgZI GCGATG 1 cut(s) 457
BtsCI GGATG 1 cut(s) 544
BtsI GCAGTG 1 cut(s) 428
BtsIMutI CAGTG 2 cut(s) 179, 428
Cac8I GCNNGC 1 cut(s) 161
Cfr13I GGNCC 3 cut(s) 55, 135, 425
ClaI ATCGAT 1 cut(s) 100
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 3 cut(s) 164, 221, 590
CviJI RGCY 6 cut(s) 47, 136, 286, 367, 384, 584
CviKI_1 RGCY 6 cut(s) 47, 136, 286, 367, 384, 584
DpnI GATC 4 cut(s) 99, 186, 227, 275
DpnII GATC 4 cut(s) 97, 184, 225, 273
DraIII CACNNNGTG 1 cut(s) 500
Eco47I GGWCC 2 cut(s) 55, 425
Eco88I CYCGRG 1 cut(s) 38
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 3 cut(s) 167, 224, 593
FaiI YATR 7 cut(s) 140, 165, 222, 266, 359, 480, 591
FatI CATG 3 cut(s) 163, 220, 589
Fnu4HI GCNGC 2 cut(s) 35, 122
FokI GGATG 1 cut(s) 551
Fsp4HI GCNGC 2 cut(s) 35, 122
GluI GCNGC 2 cut(s) 35, 122
HaeIII GGCC 1 cut(s) 136
Hin1II CATG 3 cut(s) 167, 224, 593
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 5 cut(s) 102, 146, 241, 340, 574
Hpy166II GTNNAC 2 cut(s) 177, 428
Hpy188I TCNGA 2 cut(s) 7, 596
Hpy188III TCNNGA 4 cut(s) 232, 245, 277, 578
Hpy8I GTNNAC 2 cut(s) 177, 428
HpyAV CCTTC 1 cut(s) 529
HpyCH4V TGCA 9 cut(s) 124, 177, 210, 261, 316, 332, 433, 471, 494
Hsp92II CATG 3 cut(s) 167, 224, 593
Kzo9I GATC 4 cut(s) 97, 184, 225, 273
LmnI GCTCC 1 cut(s) 319
LpnPI CCDG 8 cut(s) 167, 173, 188, 302, 336, 366, 504, 510
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 2 cut(s) 480, 529
MaeIII GTNAC 1 cut(s) 212
MalI GATC 4 cut(s) 99, 186, 227, 275
MbiI CCGCTC 1 cut(s) 37
MboI GATC 4 cut(s) 97, 184, 225, 273
MboII GAAGA 4 cut(s) 20, 335, 476, 532
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 3 cut(s) 289, 307, 323
MlyI GAGTC 2 cut(s) 155, 583
MnlI CCTC 9 cut(s) 34, 37, 241, 330, 498, 501, 507, 537, 574
MseI TTAA 6 cut(s) 155, 288, 306, 399, 513, 549
MspCI CTTAAG 1 cut(s) 398
NdeII GATC 4 cut(s) 97, 184, 225, 273
NlaIII CATG 3 cut(s) 167, 224, 593
PaeR7I CTCGAG 1 cut(s) 38
PfeI GAWTC 3 cut(s) 102, 241, 340
PkrI GCNGC 2 cut(s) 36, 123
Ple19I CGATCG 1 cut(s) 100
PleI GAGTC 2 cut(s) 154, 582
PpsI GAGTC 2 cut(s) 154, 582
PshBI ATTAAT 1 cut(s) 306
PspPI GGNCC 3 cut(s) 55, 135, 425
PspXI VCTCGAGB 1 cut(s) 38
PstI CTGCAG 1 cut(s) 318
PsuI RGATCY 1 cut(s) 184
PvuI CGATCG 1 cut(s) 100
SaqAI TTAA 6 cut(s) 155, 288, 306, 399, 513, 549
SatI GCNGC 2 cut(s) 35, 122
Sau3AI GATC 4 cut(s) 97, 184, 225, 273
Sau96I GGNCC 3 cut(s) 55, 135, 425
SchI GAGTC 2 cut(s) 155, 583
SduI GDGCHC 1 cut(s) 179
SetI ASST 2 cut(s) 288, 499
SfaNI GCATC 2 cut(s) 480, 529
SfcI CTRYAG 1 cut(s) 314
Sfr274I CTCGAG 1 cut(s) 38
SinI GGWCC 2 cut(s) 55, 425
SlaI CTCGAG 1 cut(s) 38
SmlI CTYRAG 3 cut(s) 38, 398, 578
SmoI CTYRAG 3 cut(s) 38, 398, 578
Sse9I AATT 3 cut(s) 289, 307, 323
SsiI CCGC 2 cut(s) 35, 505
TaqI TCGA 2 cut(s) 39, 100
TasI AATT 3 cut(s) 289, 307, 323
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 3 cut(s) 102, 241, 340
Tru1I TTAA 6 cut(s) 155, 288, 306, 399, 513, 549
Tru9I TTAA 6 cut(s) 155, 288, 306, 399, 513, 549
TscAI CASTG 2 cut(s) 186, 435
TseI GCWGC 1 cut(s) 121
TspDTI ATGAA 3 cut(s) 296, 548, 578
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 2 cut(s) 186, 435
Vha464I CTTAAG 1 cut(s) 398
VneI GTGCAC 1 cut(s) 175
VpaK11BI GGWCC 2 cut(s) 55, 425
VspI ATTAAT 1 cut(s) 306
XhoI CTCGAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.