FvH4_3g31360

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000128
Physical Location & Seq
Forward (+)
25410493 .. 25414359
3867 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g31360.t1

Sequence Viewer

Length: 3477 bp
ATGGCTAAAAGGATGATGCTTGAAACGTCGTCAGCAAGTACTACGTCAGAAGAAGGGTATACGTCTCTAAAGGATAATCCTTTATTTGTCTCGTGGAAGGGTAATCCCTTATATGAGACCTTGGTTCCAACTGAAGGCTCCTTACATGCTTTGATACCTCAATCAGTCGCAAGGTCTAGTGAGAGTTGTGACTCTGATTCTGATAACGTTCAAGTTCTCATGGCAAGCTCGTCAAGCCCAGAAGAGCAACTGAAACAGCAGATGGAGGAGTTGACAAAGAAGTTAGCTCAACTTGAACGCGATGCTCTACAGAGGCAAGTTGAAAGAGAAGTAGAAGCTAAGAGGATAGCAGATATCGAGGCCGAGTCGAAGAGGAGATTAGCTGAAAAAGAAGCCGAGGTTGTTGAGCTTATAGCAAAATGTGCTGCACTCATGGAAAAGAAAGGGCAGGAAGGTCCTGGTCCCTCATTTGTTAAAGATAACGATAATGCGCTCTCGCCTGAAGGGATTAAGAACATGATAGCTGAAGGGATCAGAGACTTCCAAATGTCTCTGTCTACACCAGTCCTTGGATACCGTAAGCCATATCCAGACCATTATGATGCGATACCTTTTCCTAAGGGATATCAAAGGCCAACGTTCGACAAGTTTGACGGGATAAATAGTTCCCCTCGAGAGCATTTAGCTCACTTTTATTCAGCTTGTGGAGAAGCGTCACAAATCGATGCCTTTTTGGTCAGACAGTTTGTACAATCCCTCAAAGGTGCTGCGTTCACGTGGTACACTCAGCTAGAGCCAGGTTCCATTCAAACATGGGATGATATGCAAAGAGCATTTTTGGCACAGTTTGTCAGCTCTAAGAAGAAAGTTTCAATTATTGACCTGGCAGACACAAGACAAAAAGTTGGAGAAAGTTCTACCGAATTCATCACAAGGTGGAGAAGTCTTAATCTCCAATGTCCTGACAAGTTATCTGAGACCTCTGCTGTTCAGATGTGTGCTAATAACCTCCTCCCAGAGATCGCGACATATGTTGGCACAGCTGAACCCCAAACTTTTGATGCATTGGTCTCCAAAGCAAGCAATGTCGAAAGGCAAATTGCTCGCCAAAAGTCATCTTCAAAAATTCAAATTGCGGATAAGAAGAACGATTCCAAGAAGCCAGTCGTCAAGAAAGGGGAAACAATGGCAACTTTCGTCAGGAATGAAAAAAAAATGACAATGTCAAAAGCGATTTTCGATGAGCTCATTGCATCTGAAAGCATTAAAATTCCTGAATCAAAACGACCTGCTGAAATGAACAAGACCAATGATCCTAAATATTGTCGGTATCACCGCATAGTAAGTCATACGTTGAAGGACTGTTATATTCTTAAGGACATAATTCAAGATATGATTAACAAAAAGGAGATCGTGACAGCAATCGCAAATGTAACTACAACTGGTTTGTCTAGACCAGATGGTGCCATATCAGTCGCGTTCAAGCTTATGACAGCACCAAGTTTTGATGTATGGGAGGATTCAGATGGGTTGGAAGATTCAGATGGAGAATGGTGCAGGGCAGAAAATAATGCAAGACAACTTCGTCCCCCTATTGTGAAGACCCCTTCTTCTTCCTCAAGAATCAAATATCGCCGCAAGTCATCTTCAAAAAATAAGAAGAAAGGAAGAACTGCAAAGAAAGTTCAAATCCAAAACACGGAGGAGTACAAGCAGTCCCCGAGAACTCCGATTACTCTTGGGGACTTCCTACCAAAAGAGCTTTTTGGGAAGGACCAACAAAGAAAAGACGATGAGGACGTACTTCAATGTTGCATGGTAACTATTAATGCAGTTTCCCCTTCCAACTCAGATGCACATGATGAAAGTGCACATGAAGTTGATCAAATTCAGCTACGCTCTGGCTTGGTTGTCCCATCACCTGTGCAAAAGGGTGGAAAATCACTACCCATGAAATTTAGGGTAAAAAATGATACAAAGAAGGTGACTGATAGGGTAAAAACTACATCAAAAGAGTCTCCTCAGAAGACCAAAGAAACTCACCAAAAGAGCAAATATGATGTACTATCACACCTGAAACGTATCCCCTCACTTTTAAGTGTGTATGACGTTTTGTGTATGACAAGGGAGTTAAGAGAAGCATTGATCGAAGCGTTGTTGAGCCCAGATTTGTATAAAGAGGATGTTGTTGAAGTTGATACGACCACCGCAAGAGTTTGTCCCCATTGCAGAAATTGCATCTCGTTTCTAGAGGAGGATTTGCTGTTGAAAGGGGAACACCATAACAGACCTCTCTACGTCACAGGACTTGTGGGTAATACAACAATCAATAGAATTTTACTTGACTGTGGCTCGGCTGTGAACTTGTTGCCCCTCAAAACGCTTCACATTATGGGAATGAGTGCTAAACAACTTACTCCATCCATGCTCACCATCCAAGGTTTTAACCAAGCTGGTCAAAAAGCAATGGGTACGATTGTCTTACAAATGGAGATTGGGGAAATTCTCTCAAGCGCTTTGTTTCATGTGATTGATGCTCCTACGTCCTACAATGTATTGTTAGGGCGCCCATGGCTCCATAATTATGGTGTCGTCCCTTCTACACTTCACCAATGCTTTAAGTGGTTGGAAAATGGAGAAGTCAAAACAGTCGTGGCAGATGCTGACCCTTTTAAAGGCGAAGAGCCAACGGAGCTTCCTGAAGTACTTGTGGAAGAAACTCCCAAACACGTGCTAGAAGCAAGGAAATTGGAAGCGTCGAAGCCGTCAAAACTTCGGGTCAGAGTAACTCCCCCGGTATTGTCCTCAAAAGGAAAGGCTCCAAGCTCAGAAAAATCAAAACCAAAGATGGTCTTCAAGTATGTGAAAATAGACCAACGAGAACCTAGGCAAAAAGCCATAACACCTATCAAGGACACAATGGAGACGCTCTGCACGACCTCTTCAAATCTGAAGTTAGCATCATCTGTTCCTTTAAAGATTAACCTTAAAGGTAGAAAGGCAATTTCAAAAGCCACTCCCCTAAAAGTCATGGTTAAGCAGGTAAATGGTATGCTTAAGGTAAAGGTTCCTGAGTCAGATTCCACCGAGTCAGGTGTTGTTACGGATCTTGAGAAGACTATGTTTCGTGAGGAAGACATGACACAAGAAGATTTGCCTCGTGTTTCTGTGTTTCGACGTCTAACAATTGATCCTCAACCTCGAGTTTCGATGTTTCAACGATTGGGTGAAATTCATCCCAAACCACAAAAGAACAGAAAGTGGAAGCTCAAGGTTAAACAACAAAGAGTACTAGTTGCTAAACAGGAGGACGAAAGTACACATGAATCTTCGTCAACGAATTGCGTTTCCATCGAAGAAAATCAAGTCGAAGACGAAGAAGAATATGAATTCATCGATAGTGCTCAACTTGCCCCGCCCCAAATAGAAGATGGAGGACAAGCGACAAAGAATTTCAAGACTACAAGAATCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 Retrotrans_gag rve

Protein Analysis

1159

Amino Acids

129.99

Weight (kDa)

8.64

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 249 - 335 5.7e-13 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1584
AatII GACGTC 1 cut(s) 3181
Acc36I ACCTGC 2 cut(s) 1297, 3031
AccB1I GGYRCC 2 cut(s) 1463, 2565
AccB7I CCANNNNNTGG 1 cut(s) 569
AccI GTMKAC 2 cut(s) 59, 557
AccII CGCG 3 cut(s) 300, 1025, 1478
AciI CCGC 5 cut(s) 1136, 1336, 1636, 2208, 3416
AclI AACGTT 2 cut(s) 207, 638
AclWI GGATC 4 cut(s) 539, 1307, 3114, 3185
AcuI CTGAAG 5 cut(s) 153, 522, 546, 2721, 2972
AcvI CACGTG 2 cut(s) 777, 2731
AcyI GRCGYC 2 cut(s) 2566, 3178
AdeI CACNNNGTG 1 cut(s) 936
AfeI AGCGCT 1 cut(s) 2515
AfiI CCNNNNNNNGG 4 cut(s) 134, 569, 1699, 2675
AflII CTTAAG 2 cut(s) 1373, 3056
AflIII ACRYGT 1 cut(s) 2728
AhlI ACTAGT 1 cut(s) 3292
AjnI CCWGG 3 cut(s) 457, 796, 882
AloI GAACNNNNNNTCC 2 cut(s) 649, 681
Alw21I GWGCWC 3 cut(s) 1248, 1873, 3406
Alw26I GTCTC 9 cut(s) 69, 94, 110, 531, 555, 971, 1075, 2022, 2918
Alw44I GTGCAC 1 cut(s) 1869
AlwI GGATC 4 cut(s) 539, 1307, 3114, 3185
AlwNI CAGNNNCTG 1 cut(s) 2663
Ama87I CYCGRG 3 cut(s) 672, 1720, 3201
Aor51HI AGCGCT 1 cut(s) 2515
AoxI GGCC 2 cut(s) 360, 632
ApaLI GTGCAC 1 cut(s) 1869
ApeKI GCWGC 2 cut(s) 425, 767
AseI ATTAAT 1 cut(s) 1827
AspA2I CCTAGG 1 cut(s) 2885
AspLEI GCGC 3 cut(s) 493, 2516, 2568
AspS9I GGNCC 3 cut(s) 455, 461, 1774
AsuC2I CCSGG 1 cut(s) 2795
AsuHPI GGTGA 7 cut(s) 1325, 1911, 1996, 2033, 2422, 2600, 3239
AvaI CYCGRG 3 cut(s) 672, 1720, 3201
AvaII GGWCC 3 cut(s) 455, 461, 1774
AvrII CCTAGG 1 cut(s) 2885
AxyI CCTNAGG 1 cut(s) 618
BaeGI GKGCMC 1 cut(s) 1873
BaeI ACNNNNGTAYC 2 cut(s) 2463, 2496
BanI GGYRCC 2 cut(s) 1463, 2565
BanII GRGCYC 2 cut(s) 1248, 2165
BarI GAAGNNNNNNTAC 2 cut(s) 126, 158
BauI CACGAG 2 cut(s) 91, 3159
BbrPI CACGTG 2 cut(s) 777, 2731
BbsI GAAGAC 6 cut(s) 1607, 2033, 2845, 3122, 3141, 3378
Bbv12I GWGCWC 3 cut(s) 1248, 1873, 3406
BbvI GCAGC 2 cut(s) 412, 754
BceAI ACGGC 1 cut(s) 2749
BcgI CGANNNNNNTGC 2 cut(s) 1085, 1119
BciT130I CCWGG 3 cut(s) 459, 798, 884
BciVI GTATCC 2 cut(s) 566, 2093
BclI TGATCA 1 cut(s) 1882
BcnI CCSGG 1 cut(s) 2795
BcoDI GTCTC 9 cut(s) 69, 94, 110, 531, 555, 971, 1075, 2022, 2918
BcuI ACTAGT 1 cut(s) 3292
BfaI CTAG 8 cut(s) 177, 791, 1452, 2249, 2735, 2886, 3293, 3475
BfmI CTRYAG 1 cut(s) 308
BfoI RGCGCY 2 cut(s) 2517, 2569
BfrI CTTAAG 2 cut(s) 1373, 3056
BfuAI ACCTGC 2 cut(s) 1297, 3031
BfuI GTATCC 2 cut(s) 566, 2093
BisI GCNGC 3 cut(s) 426, 768, 1636
BlnI CCTAGG 1 cut(s) 2885
BlsI GCNGC 3 cut(s) 427, 769, 1637
BmcAI AGTACT 3 cut(s) 40, 2706, 3291
Bme1390I CCNGG 4 cut(s) 459, 798, 884, 2795
Bme18I GGWCC 3 cut(s) 455, 461, 1774
BmeT110I CYCGRG 3 cut(s) 672, 1720, 3201
BmgT120I GGNCC 3 cut(s) 455, 461, 1774
BmiI GGNNCC 9 cut(s) 126, 139, 463, 802, 1465, 2567, 2576, 2820, 3069
BmrFI CCNGG 4 cut(s) 459, 798, 884, 2795
BpiI GAAGAC 6 cut(s) 1607, 2033, 2845, 3122, 3141, 3378
BpuEI CTTGAG 4 cut(s) 1603, 2494, 3131, 3254
BpuMI CCSGG 1 cut(s) 2795
Bsa29I ATCGAT 2 cut(s) 723, 3396
BsaAI YACGTR 2 cut(s) 777, 2731
BsaHI GRCGYC 2 cut(s) 2566, 3178
BsaI GGTCTC 3 cut(s) 110, 971, 1075
BsaJI CCNNGG 7 cut(s) 120, 396, 568, 2437, 2570, 2793, 2885
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 569, 1699, 2675
Bse1I ACTGG 3 cut(s) 563, 1163, 1447
Bse21I CCTNAGG 1 cut(s) 618
Bse3DI GCAATG 4 cut(s) 1090, 1248, 2224, 2472
BseBI CCWGG 3 cut(s) 459, 798, 884
BseCI ATCGAT 2 cut(s) 723, 3396
BseDI CCNNGG 7 cut(s) 120, 396, 568, 2437, 2570, 2793, 2885
BseGI GGATG 6 cut(s) 18, 823, 2188, 2420, 2433, 3235
BseLI CCNNNNNNNGG 4 cut(s) 134, 569, 1699, 2675
BseMI GCAATG 4 cut(s) 1090, 1248, 2224, 2472
BseMII CTCAG 6 cut(s) 800, 966, 1863, 2036, 2841, 3063
BseNI ACTGG 3 cut(s) 563, 1163, 1447
BseRI GAGGAG 6 cut(s) 281, 388, 1001, 1718, 2010, 2267
BseSI GKGCMC 1 cut(s) 1873
BseXI GCAGC 2 cut(s) 412, 754
BsgI GTGCAG 3 cut(s) 411, 1576, 2917
Bsh1236I CGCG 3 cut(s) 300, 1025, 1478
BshFI GGCC 2 cut(s) 362, 634
BshNI GGYRCC 2 cut(s) 1463, 2565
BshVI ATCGAT 2 cut(s) 723, 3396
BsiHKAI GWGCWC 3 cut(s) 1248, 1873, 3406
BsiHKCI CYCGRG 3 cut(s) 672, 1720, 3201
BsiSI CCGG 1 cut(s) 2795
BslFI GGGAC 7 cut(s) 447, 1572, 1702, 1757, 1898, 2205, 2579
BslI CCNNNNNNNGG 4 cut(s) 134, 569, 1699, 2675
BsmAI GTCTC 9 cut(s) 69, 94, 110, 531, 555, 971, 1075, 2022, 2918
BsmBI CGTCTC 2 cut(s) 69, 2918
BsmFI GGGAC 7 cut(s) 447, 1572, 1702, 1757, 1898, 2205, 2579
BsnI GGCC 2 cut(s) 362, 634
Bso31I GGTCTC 3 cut(s) 110, 971, 1075
BsoBI CYCGRG 3 cut(s) 672, 1720, 3201
Bsp1286I GDGCHC 4 cut(s) 1248, 1873, 2165, 3406
Bsp1407I TGTACA 1 cut(s) 748
Bsp143I GATC 8 cut(s) 531, 1020, 1312, 1410, 1882, 2145, 3106, 3190
Bsp19I CCATGG 1 cut(s) 2570
Bsp68I TCGCGA 1 cut(s) 1025
BspACI CCGC 5 cut(s) 1136, 1336, 1636, 2208, 3416
BspANI GGCC 2 cut(s) 362, 634
BspCNI CTCAG 6 cut(s) 799, 967, 1862, 2035, 2840, 3064
BspDI ATCGAT 2 cut(s) 723, 3396
BspFNI CGCG 3 cut(s) 300, 1025, 1478
BspLI GGNNCC 9 cut(s) 126, 139, 463, 802, 1465, 2567, 2576, 2820, 3069
BspMI ACCTGC 2 cut(s) 1297, 3031
BspPI GGATC 4 cut(s) 539, 1307, 3114, 3185
BspQI GCTCTTC 2 cut(s) 237, 2676
BspT107I GGYRCC 2 cut(s) 1463, 2565
BspTI CTTAAG 2 cut(s) 1373, 3056
BspTNI GGTCTC 3 cut(s) 110, 971, 1075
BsrDI GCAATG 4 cut(s) 1090, 1248, 2224, 2472
BsrGI TGTACA 1 cut(s) 748
BsrI ACTGG 3 cut(s) 563, 1163, 1447
BssECI CCNNGG 7 cut(s) 120, 396, 568, 2437, 2570, 2793, 2885
BssMI GATC 8 cut(s) 531, 1020, 1312, 1410, 1882, 2145, 3106, 3190
BssNAI GTATAC 1 cut(s) 60
BssNI GRCGYC 2 cut(s) 2566, 3178
BssSI CACGAG 2 cut(s) 91, 3159
BssT1I CCWWGG 5 cut(s) 120, 568, 2437, 2570, 2885
Bst1107I GTATAC 1 cut(s) 60
Bst2BI CACGAG 2 cut(s) 91, 3159
Bst2UI CCWGG 3 cut(s) 459, 798, 884
Bst4CI ACNGT 6 cut(s) 578, 744, 846, 1364, 2348, 2650
Bst6I CTCTTC 4 cut(s) 237, 365, 2676, 2947
BstACI GRCGYC 2 cut(s) 2566, 3178
BstAFI CTTAAG 2 cut(s) 1373, 3056
BstAPI GCANNNNNTGC 2 cut(s) 422, 2235
BstAUI TGTACA 1 cut(s) 748
BstBAI YACGTR 2 cut(s) 777, 2731
BstC8I GCNNGC 3 cut(s) 226, 1081, 1105
BstDEI CTNAG 9 cut(s) 339, 618, 786, 858, 975, 1849, 2022, 2827, 3072
BstDSI CCRYGG 1 cut(s) 2570
BstENI CCTNNNNNAGG 1 cut(s) 2673
BstF5I GGATG 6 cut(s) 18, 823, 2188, 2420, 2433, 3235
BstFNI CGCG 3 cut(s) 300, 1025, 1478
BstH2I RGCGCY 2 cut(s) 2517, 2569
BstHHI GCGC 3 cut(s) 493, 2516, 2568
BstKTI GATC 8 cut(s) 534, 1023, 1315, 1413, 1885, 2148, 3109, 3193
BstMAI GTCTC 9 cut(s) 69, 94, 110, 531, 555, 971, 1075, 2022, 2918
BstMBI GATC 8 cut(s) 531, 1020, 1312, 1410, 1882, 2145, 3106, 3190
BstMWI GCNNNNNNNGC 7 cut(s) 234, 422, 839, 2235, 2572, 2692, 3410
BstNI CCWGG 3 cut(s) 459, 798, 884
BstNSI RCATGY 1 cut(s) 149
BstSCI CCNGG 4 cut(s) 457, 796, 882, 2793
BstSFI CTRYAG 1 cut(s) 308
BstSLI GKGCMC 1 cut(s) 1873
BstUI CGCG 3 cut(s) 300, 1025, 1478
BstV1I GCAGC 2 cut(s) 412, 754
BstV2I GAAGAC 6 cut(s) 1607, 2033, 2845, 3122, 3141, 3378
BstX2I RGATCY 1 cut(s) 3106
BstXI CCANNNNNNTGG 1 cut(s) 2585
BstYI RGATCY 1 cut(s) 3106
BstZ17I GTATAC 1 cut(s) 60
Bsu15I ATCGAT 2 cut(s) 723, 3396
Bsu36I CCTNAGG 1 cut(s) 618
BsuI GTATCC 2 cut(s) 566, 2093
BsuRI GGCC 2 cut(s) 362, 634
BsuTUI ATCGAT 2 cut(s) 723, 3396
BtgI CCRYGG 1 cut(s) 2570
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 6 cut(s) 18, 823, 2188, 2420, 2433, 3235
BtuMI TCGCGA 1 cut(s) 1025
BveI ACCTGC 2 cut(s) 1297, 3031
Cac8I GCNNGC 3 cut(s) 226, 1081, 1105
CaiI CAGNNNCTG 1 cut(s) 2663
CfoI GCGC 3 cut(s) 493, 2516, 2568
Cfr13I GGNCC 3 cut(s) 455, 461, 1774
ClaI ATCGAT 2 cut(s) 723, 3396
CseI GACGC 3 cut(s) 702, 2745, 2935
DdeI CTNAG 9 cut(s) 339, 618, 786, 858, 975, 1849, 2022, 2827, 3072
DinI GGCGCC 1 cut(s) 2567
DpnI GATC 8 cut(s) 533, 1022, 1314, 1412, 1884, 2147, 3108, 3192
DpnII GATC 8 cut(s) 531, 1020, 1312, 1410, 1882, 2145, 3106, 3190
DraI TTTAAA 2 cut(s) 2674, 2976
DraIII CACNNNGTG 1 cut(s) 936
DrdI GACNNNNNNGTC 1 cut(s) 1584
DseDI GACNNNNNNGTC 1 cut(s) 1584
Eam1104I CTCTTC 4 cut(s) 237, 365, 2676, 2947
EarI CTCTTC 4 cut(s) 237, 365, 2676, 2947
Ecl136II GAGCTC 1 cut(s) 1246
Eco130I CCWWGG 5 cut(s) 120, 568, 2437, 2570, 2885
Eco24I GRGCYC 2 cut(s) 1248, 2165
Eco31I GGTCTC 3 cut(s) 110, 971, 1075
Eco32I GATATC 2 cut(s) 355, 626
Eco47I GGWCC 3 cut(s) 455, 461, 1774
Eco47III AGCGCT 1 cut(s) 2515
Eco53kI GAGCTC 1 cut(s) 1246
Eco57I CTGAAG 5 cut(s) 153, 522, 546, 2721, 2972
Eco72I CACGTG 2 cut(s) 777, 2731
Eco81I CCTNAGG 1 cut(s) 618
Eco88I CYCGRG 3 cut(s) 672, 1720, 3201
EcoICRI GAGCTC 1 cut(s) 1246
EcoNI CCTNNNNNAGG 1 cut(s) 2673
EcoO109I RGGNCCY 1 cut(s) 455
EcoRI GAATTC 2 cut(s) 923, 3389
EcoRII CCWGG 3 cut(s) 457, 796, 882
EcoRV GATATC 2 cut(s) 355, 626
EcoT14I CCWWGG 5 cut(s) 120, 568, 2437, 2570, 2885
EcoT22I ATGCAT 1 cut(s) 1066
EcoT38I GRGCYC 2 cut(s) 1248, 2165
EgeI GGCGCC 1 cut(s) 2567
EheI GGCGCC 1 cut(s) 2567
ErhI CCWWGG 5 cut(s) 120, 568, 2437, 2570, 2885
Esp3I CGTCTC 2 cut(s) 69, 2918
FalI AAGNNNNNCTT 2 cut(s) 2837, 2869
FaqI GGGAC 7 cut(s) 447, 1572, 1702, 1757, 1898, 2205, 2579
FauI CCCGC 1 cut(s) 3423
FauNDI CATATG 1 cut(s) 1030
FbaI TGATCA 1 cut(s) 1882
FblI GTMKAC 2 cut(s) 59, 557
Fnu4HI GCNGC 3 cut(s) 426, 768, 1636
FokI GGATG 6 cut(s) 25, 830, 2195, 2407, 2420, 3222
FriOI GRGCYC 2 cut(s) 1248, 2165
Fsp4HI GCNGC 3 cut(s) 426, 768, 1636
FspBI CTAG 8 cut(s) 177, 791, 1452, 2249, 2735, 2886, 3293, 3475
GlaI GCGC 3 cut(s) 492, 2515, 2567
GluI GCNGC 3 cut(s) 426, 768, 1636
HaeII RGCGCY 2 cut(s) 2517, 2569
HaeIII GGCC 2 cut(s) 362, 634
HapII CCGG 1 cut(s) 2795
HgaI GACGC 3 cut(s) 702, 2745, 2935
HhaI GCGC 3 cut(s) 493, 2516, 2568
Hin1I GRCGYC 2 cut(s) 2566, 3178
Hin6I GCGC 3 cut(s) 491, 2514, 2566
HinP1I GCGC 3 cut(s) 491, 2514, 2566
HincII GTYRAC 2 cut(s) 273, 3336
HindII GTYRAC 2 cut(s) 273, 3336
HindIII AAGCTT 1 cut(s) 1484
HpaII CCGG 1 cut(s) 2795
HphI GGTGA 7 cut(s) 1325, 1911, 1996, 2033, 2422, 2600, 3239
Hpy166II GTNNAC 9 cut(s) 60, 273, 558, 774, 783, 1871, 2362, 3320, 3336
Hpy8I GTNNAC 9 cut(s) 60, 273, 558, 774, 783, 1871, 2362, 3320, 3336
Hpy99I CGWCG 3 cut(s) 31, 2761, 3180
HpyCH4III ACNGT 6 cut(s) 578, 744, 846, 1364, 2348, 2650
HpyF10VI GCNNNNNNNGC 7 cut(s) 234, 422, 839, 2235, 2572, 2692, 3410
HpyF3I CTNAG 9 cut(s) 339, 618, 786, 858, 975, 1849, 2022, 2827, 3072
Hsp92I GRCGYC 2 cut(s) 2566, 3178
HspAI GCGC 3 cut(s) 491, 2514, 2566
KasI GGCGCC 1 cut(s) 2565
Ksp22I TGATCA 1 cut(s) 1882
Kzo9I GATC 8 cut(s) 531, 1020, 1312, 1410, 1882, 2145, 3106, 3190
LguI GCTCTTC 2 cut(s) 237, 2676
LmnI GCTCC 5 cut(s) 143, 2542, 2580, 2692, 2824
Lsp1109I GCAGC 2 cut(s) 412, 754
MaeI CTAG 8 cut(s) 177, 791, 1452, 2249, 2735, 2886, 3293, 3475
MaeIII GTNAC 9 cut(s) 188, 714, 1414, 1432, 1819, 1984, 2299, 2785, 3100
MalI GATC 8 cut(s) 533, 1022, 1314, 1412, 1884, 2147, 3108, 3192
MboI GATC 8 cut(s) 531, 1020, 1312, 1410, 1882, 2145, 3106, 3190
MfeI CAATTG 1 cut(s) 3186
MflI RGATCY 1 cut(s) 3106
MhlI GDGCHC 4 cut(s) 1248, 1873, 2165, 3406
Mly113I GGCGCC 1 cut(s) 2566
MlyI GAGTC 5 cut(s) 185, 374, 2024, 3083, 3098
MmeI TCCRAC 5 cut(s) 152, 886, 1512, 1869, 2607
Mph1103I ATGCAT 1 cut(s) 1066
MslI CAYNNNNRTG 2 cut(s) 600, 2583
MspA1I CMGCKG 1 cut(s) 1043
MspCI CTTAAG 2 cut(s) 1373, 3056
MspI CCGG 1 cut(s) 2795
MspR9I CCNGG 4 cut(s) 459, 798, 884, 2795
MunI CAATTG 1 cut(s) 3186
MvaI CCWGG 3 cut(s) 459, 798, 884
MvnI CGCG 3 cut(s) 300, 1025, 1478
MwoI GCNNNNNNNGC 7 cut(s) 234, 422, 839, 2235, 2572, 2692, 3410
NarI GGCGCC 1 cut(s) 2566
NciI CCSGG 1 cut(s) 2795
NcoI CCATGG 1 cut(s) 2570
NdeI CATATG 1 cut(s) 1030
NdeII GATC 8 cut(s) 531, 1020, 1312, 1410, 1882, 2145, 3106, 3190
NlaIV GGNNCC 9 cut(s) 126, 139, 463, 802, 1465, 2567, 2576, 2820, 3069
NmeAIII GCCGAG 3 cut(s) 388, 421, 2333
NmuCI GTSAC 5 cut(s) 188, 714, 1414, 1984, 2299
NruI TCGCGA 1 cut(s) 1025
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 1 cut(s) 149
PaeR7I CTCGAG 2 cut(s) 672, 3201
PciSI GCTCTTC 2 cut(s) 237, 2676
PcsI WCGNNNNNNNCGW 2 cut(s) 1797, 2933
PfeI GAWTC 9 cut(s) 197, 1151, 1277, 1520, 1538, 1623, 3080, 3326, 3468
PflFI GACNNNGTC 1 cut(s) 1222
PflMI CCANNNNNTGG 1 cut(s) 569
PkrI GCNGC 3 cut(s) 427, 769, 1637
PleI GAGTC 5 cut(s) 185, 373, 2023, 3082, 3097
PluTI GGCGCC 1 cut(s) 2569
PmaCI CACGTG 2 cut(s) 777, 2731
PmlI CACGTG 2 cut(s) 777, 2731
PpsI GAGTC 5 cut(s) 185, 373, 2023, 3082, 3097
Ppu21I YACGTR 2 cut(s) 777, 2731
PpuMI RGGWCCY 1 cut(s) 455
PshBI ATTAAT 1 cut(s) 1827
Psp124BI GAGCTC 1 cut(s) 1248
Psp1406I AACGTT 2 cut(s) 207, 638
Psp5II RGGWCCY 1 cut(s) 455
Psp6I CCWGG 3 cut(s) 457, 796, 882
PspCI CACGTG 2 cut(s) 777, 2731
PspGI CCWGG 3 cut(s) 457, 796, 882
PspN4I GGNNCC 9 cut(s) 126, 139, 463, 802, 1465, 2567, 2576, 2820, 3069
PspPI GGNCC 3 cut(s) 455, 461, 1774
PspPPI RGGWCCY 1 cut(s) 455
PspXI VCTCGAGB 1 cut(s) 3201
PsrI GAACNNNNNNTAC 2 cut(s) 1717, 1749
PstNI CAGNNNCTG 1 cut(s) 2663
PsuI RGATCY 1 cut(s) 3106
PsyI GACNNNGTC 1 cut(s) 1222
PvuII CAGCTG 1 cut(s) 1043
RruI TCGCGA 1 cut(s) 1025
RseI CAYNNNNRTG 2 cut(s) 600, 2583
SacI GAGCTC 1 cut(s) 1248
SapI GCTCTTC 2 cut(s) 237, 2676
SatI GCNGC 3 cut(s) 426, 768, 1636
Sau3AI GATC 8 cut(s) 531, 1020, 1312, 1410, 1882, 2145, 3106, 3190
Sau96I GGNCC 3 cut(s) 455, 461, 1774
ScaI AGTACT 3 cut(s) 40, 2706, 3291
SchI GAGTC 5 cut(s) 185, 374, 2024, 3083, 3098
ScrFI CCNGG 4 cut(s) 459, 798, 884, 2795
SduI GDGCHC 4 cut(s) 1248, 1873, 2165, 3406
SfcI CTRYAG 1 cut(s) 308
SfoI GGCGCC 1 cut(s) 2567
Sfr274I CTCGAG 2 cut(s) 672, 3201
SinI GGWCC 3 cut(s) 455, 461, 1774
SlaI CTCGAG 2 cut(s) 672, 3201
SmiMI CAYNNNNRTG 2 cut(s) 600, 2583
SmlI CTYRAG 8 cut(s) 672, 1373, 1618, 2509, 3056, 3110, 3201, 3269
SmoI CTYRAG 8 cut(s) 672, 1373, 1618, 2509, 3056, 3110, 3201, 3269
SpeI ACTAGT 1 cut(s) 3292
SsiI CCGC 5 cut(s) 1136, 1336, 1636, 2208, 3416
SspDI GGCGCC 1 cut(s) 2565
SspI AATATT 1 cut(s) 1322
SspMI CTAG 8 cut(s) 177, 791, 1452, 2249, 2735, 2886, 3293, 3475
SstI GAGCTC 1 cut(s) 1248
StyD4I CCNGG 4 cut(s) 457, 796, 882, 2793
StyI CCWWGG 5 cut(s) 120, 568, 2437, 2570, 2885
TaaI ACNGT 6 cut(s) 578, 744, 846, 1364, 2348, 2650
TatI WGTACW 7 cut(s) 38, 748, 1707, 2062, 2704, 3289, 3317
TauI GCSGC 1 cut(s) 1638
TfiI GAWTC 9 cut(s) 197, 1151, 1277, 1520, 1538, 1623, 3080, 3326, 3468
TseFI GTSAC 5 cut(s) 188, 714, 1414, 1984, 2299
TseI GCWGC 2 cut(s) 425, 767
Tsp45I GTSAC 5 cut(s) 188, 714, 1414, 1984, 2299
TspGWI ACGGA 3 cut(s) 1715, 2705, 3119
Tth111I GACNNNGTC 1 cut(s) 1222
Van91I CCANNNNNTGG 1 cut(s) 569
Vha464I CTTAAG 2 cut(s) 1373, 3056
VneI GTGCAC 1 cut(s) 1869
VpaK11BI GGWCC 3 cut(s) 455, 461, 1774
VspI ATTAAT 1 cut(s) 1827
XagI CCTNNNNNAGG 1 cut(s) 2673
XbaI TCTAGA 2 cut(s) 1451, 2248
XceI RCATGY 1 cut(s) 149
XcmI CCANNNNNNNNNTGG 1 cut(s) 3428
XhoI CTCGAG 2 cut(s) 672, 3201
XmaJI CCTAGG 1 cut(s) 2885
XmiI GTMKAC 2 cut(s) 59, 557
XspI CTAG 8 cut(s) 177, 791, 1452, 2249, 2735, 2886, 3293, 3475
ZraI GACGTC 1 cut(s) 3179
ZrmI AGTACT 3 cut(s) 40, 2706, 3291
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.