pycom08g13620

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000128
Physical Location & Seq
Forward (+)
12451827 .. 12452534
708 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g13620.1

Sequence Viewer

Length: 708 bp
ATGCAAGTCATGACCACTGGAGCAACTTCAATTGAGGAGCAGTTAGCTCAGATGAATGAAGCAATCGCAAACCTTACACGGACTGTGGAAGAGAAGGACTTGCAGATTGCCACACTTGTCAACCAACTAGATGCGTGGCCCGACGTGAAAGTCGACCCAAATGTTGGTCTAGTAAAGAAAGAAGTCGACGAAGAACAGGAGCCTCCATCGGAGAAAGTCGAAGAGAAGTTGAAGCTAGACCAAGCAACGGCGTTCATGGGATCTCTCTCTATCCAGCAGCTACATGAGATGATTGCAAGCACTATCAAGACACAGTACGAAGGAAGCTCACATGACTCCGTACAGTACTCAAAGCCTTATTCCAAGAAGATTGATGCTTTGAAGATGCCAAGGGGTTATCAACCCCCAAAATTTATGCAGTTCGATGGAAAAGGCAACCCGAAGCAACATGTCGCCCATTTCATTGAAACTTGCAACAATGCTGGGACAGAGGGGGACTACCTCGTCAAACAATTCGTGCGTTCGCTGAAAAGTAATGCTTTTGACTGGTACACCGACCTCGAACCCGAGTCTATCAACAGTTGGGACCAGCTAGAAAGGGAATTCCTCAACCGCTTCTACAGCACTCGCCATACTGTAAGCATGCTAGAGCTGACCAGTACGAAACAGTGGAAAGATGAACCTGTCGTCGACTACATCAATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

236

Amino Acids

26.97

Weight (kDa)

4.99

Isoelectric Point (pI)

32.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 172 - 235 8.5e-09 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 503
AccB7I CCANNNNNTGG 1 cut(s) 164
AccI GTMKAC 3 cut(s) 153, 186, 690
AciI CCGC 1 cut(s) 613
AclWI GGATC 1 cut(s) 268
AcsI RAATTY 2 cut(s) 410, 602
AfaI GTAC 5 cut(s) 317, 342, 347, 551, 661
AfiI CCNNNNNNNGG 2 cut(s) 164, 247
AflIII ACRYGT 1 cut(s) 448
AgsI TTSAA 4 cut(s) 30, 232, 382, 467
AjiI CACGTC 1 cut(s) 145
AluBI AGCT 6 cut(s) 47, 235, 280, 327, 592, 652
AluI AGCT 6 cut(s) 47, 235, 280, 327, 592, 652
AlwI GGATC 1 cut(s) 268
Ama87I CYCGRG 1 cut(s) 566
AoxI GGCC 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 277
ApoI RAATTY 2 cut(s) 410, 602
AspS9I GGNCC 2 cut(s) 138, 586
AvaI CYCGRG 1 cut(s) 566
AvaII GGWCC 1 cut(s) 586
BbvI GCAGC 1 cut(s) 289
BccI CCATC 2 cut(s) 214, 419
BceAI ACGGC 1 cut(s) 264
BfaI CTAG 5 cut(s) 128, 170, 236, 593, 647
BfmI CTRYAG 1 cut(s) 619
BisI GCNGC 1 cut(s) 278
BlsI GCNGC 1 cut(s) 279
BmcAI AGTACT 1 cut(s) 347
Bme18I GGWCC 1 cut(s) 586
BmeT110I CYCGRG 1 cut(s) 566
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 138, 586
BmiI GGNNCC 2 cut(s) 201, 587
BmsI GCATC 3 cut(s) 121, 364, 375
BpmI CTGGAG 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 389
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 247
Bse1I ACTGG 3 cut(s) 22, 551, 657
BseDI CCNNGG 1 cut(s) 389
BseLI CCNNNNNNNGG 2 cut(s) 164, 247
BseMII CTCAG 1 cut(s) 62
BseNI ACTGG 3 cut(s) 22, 551, 657
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 1 cut(s) 289
BseYI CCCAGC 1 cut(s) 482
BshFI GGCC 1 cut(s) 139
BsiHKCI CYCGRG 1 cut(s) 566
BslFI GGGAC 3 cut(s) 499, 509, 599
BslI CCNNNNNNNGG 2 cut(s) 164, 247
BsmFI GGGAC 3 cut(s) 499, 509, 599
BsnI GGCC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 566
Bsp143I GATC 1 cut(s) 260
BspACI CCGC 1 cut(s) 613
BspANI GGCC 1 cut(s) 139
BspCNI CTCAG 1 cut(s) 61
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 2 cut(s) 201, 587
BspPI GGATC 1 cut(s) 268
BsrI ACTGG 3 cut(s) 22, 551, 657
BssECI CCNNGG 1 cut(s) 389
BssMI GATC 1 cut(s) 260
BssT1I CCWWGG 1 cut(s) 389
Bst4CI ACNGT 6 cut(s) 85, 315, 345, 581, 637, 669
Bst6I CTCTTC 2 cut(s) 84, 216
BstC8I GCNNGC 2 cut(s) 298, 644
BstDEI CTNAG 1 cut(s) 48
BstKTI GATC 1 cut(s) 263
BstMBI GATC 1 cut(s) 260
BstMWI GCNNNNNNNGC 1 cut(s) 621
BstNSI RCATGY 2 cut(s) 452, 646
BstSFI CTRYAG 1 cut(s) 619
BstV1I GCAGC 1 cut(s) 289
BstX2I RGATCY 1 cut(s) 260
BstYI RGATCY 1 cut(s) 260
BsuRI GGCC 1 cut(s) 139
BtrI CACGTC 1 cut(s) 145
BtsIMutI CAGTG 2 cut(s) 15, 674
Cac8I GCNNGC 2 cut(s) 298, 644
CciI TCATGA 1 cut(s) 9
Cfr13I GGNCC 2 cut(s) 138, 586
Csp6I GTAC 5 cut(s) 316, 341, 346, 550, 660
CviAII CATG 6 cut(s) 10, 256, 284, 332, 449, 643
CviJI RGCY 9 cut(s) 47, 139, 202, 235, 280, 327, 355, 592, 652
CviKI_1 RGCY 9 cut(s) 47, 139, 202, 235, 280, 327, 355, 592, 652
CviQI GTAC 5 cut(s) 316, 341, 346, 550, 660
DdeI CTNAG 1 cut(s) 48
DpnI GATC 1 cut(s) 262
DpnII GATC 1 cut(s) 260
DrdI GACNNNNNNGTC 2 cut(s) 149, 503
DseDI GACNNNNNNGTC 2 cut(s) 149, 503
Eam1104I CTCTTC 2 cut(s) 84, 216
EarI CTCTTC 2 cut(s) 84, 216
Eco130I CCWWGG 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 586
Eco88I CYCGRG 1 cut(s) 566
EcoRI GAATTC 1 cut(s) 602
EcoT14I CCWWGG 1 cut(s) 389
ErhI CCWWGG 1 cut(s) 389
FaeI CATG 6 cut(s) 13, 259, 287, 335, 452, 646
FaiI YATR 8 cut(s) 11, 257, 285, 333, 416, 450, 633, 644
FalI AAGNNNNNCTT 2 cut(s) 523, 555
FaqI GGGAC 3 cut(s) 499, 509, 599
FatI CATG 6 cut(s) 9, 255, 283, 331, 448, 642
FblI GTMKAC 3 cut(s) 153, 186, 690
Fnu4HI GCNGC 1 cut(s) 278
Fsp4HI GCNGC 1 cut(s) 278
FspBI CTAG 5 cut(s) 128, 170, 236, 593, 647
GluI GCNGC 1 cut(s) 278
GsaI CCCAGC 1 cut(s) 486
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 1 cut(s) 139
Hin1II CATG 6 cut(s) 13, 259, 287, 335, 452, 646
HincII GTYRAC 4 cut(s) 121, 154, 187, 691
HindII GTYRAC 4 cut(s) 121, 154, 187, 691
HinfI GANTC 2 cut(s) 335, 569
Hpy166II GTNNAC 5 cut(s) 121, 154, 187, 552, 691
Hpy188I TCNGA 2 cut(s) 51, 211
Hpy188III TCNNGA 2 cut(s) 10, 307
Hpy8I GTNNAC 5 cut(s) 121, 154, 187, 552, 691
Hpy99I CGWCG 3 cut(s) 146, 191, 692
HpyAV CCTTC 2 cut(s) 88, 314
HpyCH4III ACNGT 6 cut(s) 85, 315, 345, 581, 637, 669
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 5 cut(s) 4, 103, 296, 418, 474
HpyF10VI GCNNNNNNNGC 1 cut(s) 621
HpyF3I CTNAG 1 cut(s) 48
HpySE526I ACGT 1 cut(s) 144
Hsp92II CATG 6 cut(s) 13, 259, 287, 335, 452, 646
Kzo9I GATC 1 cut(s) 260
LmnI GCTCC 3 cut(s) 20, 37, 199
LpnPI CCDG 8 cut(s) 3, 182, 287, 468, 532, 602, 670, 696
Lsp1109I GCAGC 1 cut(s) 289
LweI GCATC 3 cut(s) 121, 364, 375
MaeI CTAG 5 cut(s) 128, 170, 236, 593, 647
MaeII ACGT 1 cut(s) 144
MalI GATC 1 cut(s) 262
MboI GATC 1 cut(s) 260
MboII GAAGA 5 cut(s) 101, 203, 233, 379, 394
MfeI CAATTG 1 cut(s) 30
MflI RGATCY 1 cut(s) 260
MluCI AATT 4 cut(s) 30, 410, 512, 602
MlyI GAGTC 2 cut(s) 329, 578
MnlI CCTC 6 cut(s) 28, 213, 484, 512, 569, 617
MunI CAATTG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 621
NdeII GATC 1 cut(s) 260
NlaIII CATG 6 cut(s) 13, 259, 287, 335, 452, 646
NlaIV GGNNCC 2 cut(s) 201, 587
NspI RCATGY 2 cut(s) 452, 646
PaeI GCATGC 1 cut(s) 646
PagI TCATGA 1 cut(s) 9
PciI ACATGT 1 cut(s) 448
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PflMI CCANNNNNTGG 1 cut(s) 164
PkrI GCNGC 1 cut(s) 279
PleI GAGTC 2 cut(s) 329, 577
PpsI GAGTC 2 cut(s) 329, 577
PscI ACATGT 1 cut(s) 448
PspFI CCCAGC 1 cut(s) 482
PspN4I GGNNCC 2 cut(s) 201, 587
PspPI GGNCC 2 cut(s) 138, 586
PsuI RGATCY 1 cut(s) 260
RsaI GTAC 5 cut(s) 317, 342, 347, 551, 661
RsaNI GTAC 5 cut(s) 316, 341, 346, 550, 660
SalI GTCGAC 3 cut(s) 152, 185, 689
SatI GCNGC 1 cut(s) 278
Sau3AI GATC 1 cut(s) 260
Sau96I GGNCC 2 cut(s) 138, 586
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 2 cut(s) 329, 578
SfaNI GCATC 3 cut(s) 121, 364, 375
SfcI CTRYAG 1 cut(s) 619
SinI GGWCC 1 cut(s) 586
SphI GCATGC 1 cut(s) 646
Sse9I AATT 4 cut(s) 30, 410, 512, 602
SsiI CCGC 1 cut(s) 613
SspMI CTAG 5 cut(s) 128, 170, 236, 593, 647
StyI CCWWGG 1 cut(s) 389
TaaI ACNGT 6 cut(s) 85, 315, 345, 581, 637, 669
TaiI ACGT 1 cut(s) 147
TaqI TCGA 6 cut(s) 153, 186, 219, 423, 561, 690
TasI AATT 4 cut(s) 30, 410, 512, 602
TatI WGTACW 1 cut(s) 345
TscAI CASTG 2 cut(s) 22, 674
TseI GCWGC 1 cut(s) 277
TspDTI ATGAA 5 cut(s) 68, 72, 244, 451, 693
TspGWI ACGGA 2 cut(s) 94, 328
TspRI CASTG 2 cut(s) 22, 674
Van91I CCANNNNNTGG 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 586
XapI RAATTY 2 cut(s) 410, 602
XceI RCATGY 2 cut(s) 452, 646
XmiI GTMKAC 3 cut(s) 153, 186, 690
XspI CTAG 5 cut(s) 128, 170, 236, 593, 647
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.