pycom505g00220

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_505
OG0000128
Physical Location & Seq
Reverse (-)
374506 .. 377147
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom505g00220.1

Sequence Viewer

Length: 1014 bp
ATGCTTGTGGATGGTGGGTCGGCCATAAACATCATGCCAAAATCAACAATGACCATAATCGGCATCAAGGTGAATGAACTATCCCTGAGCCGTCTGCTGATCCAGGGTTTTAACCAAGGAGGACAAAGGGCGATGGGCATGATCCGAGTAGAGATGACCATTGGTGAGCTCAAATCAAGCACGATATTCCACGTGATTGATGCAAGAACTTCTTACAGCTTGCTCTTAGGAAGACCTTGGATCCACGCAAATGGGGTAGTGCCGTCCACCCTTCACCAATGCCTAAAATTTTACCGAGAAGGAGTGAAGGTGATCCAAGGTGACACCAACCCATTCATCGAAGCCGAATCACACTTTGCAGACGCCAAGTTCTACATGGATGAAGACATGGTACCCGAAGCCCTTCCAGGAGAGGTCAAATCCACGGGTAGAGCAGCACCTAAAAAGCAGGAGTGGCAAGCCATGCCTAAAAGGAAAAAAGAGCAAGCCGTGCCATCTTCAAGCAAAGACGATGACAAGTTTGCTAAACCTGCAACAACCAAAGGGAGTAGGATGCCGTCAGAAGGACCGCACACACCCATATTCCAGTATACCCCGATGTCAAGAAGAAAGAATGGTCAATCTCCATTCGAAACTGGAACAAGAAAAGCCGACACACAATGGCACACGGATAATGTGAAGTTGCTCAAGACAAATGCAGTTTTGCCTCTGACACTAGGGGGTGCTAAGGTCGCAAGACTACCACAAGGCTTCATAAAAGCTCTACCAAATAAGGTGGAATCAAGCTTTCTTCCAACCAAGAGGACCGAGGAAGGTTTCGACCCAAATGCCTACAAACTCATGTCAAAGGCCGGGTACGACTTCGCTTCTTCTGCGGCGGCTGGAAAGAAGGTTTCAAACACTGTCAATGACGAAGAACGTAACCTCACCAAAACTCAAAAGAAGTTGAAAGAGCATGGTTACGGAGTTGACAACAACAAACCGGACTTGGCTTCACACCAAATGCACCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 Retrotrans_gag

Protein Analysis

338

Amino Acids

37.23

Weight (kDa)

9.59

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 538
Acc65I GGTACC 1 cut(s) 391
AccB1I GGYRCC 1 cut(s) 391
AccI GTMKAC 1 cut(s) 590
AciI CCGC 3 cut(s) 569, 875, 878
AclWI GGATC 5 cut(s) 94, 136, 235, 248, 307
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 1 cut(s) 287
AcvI CACGTG 1 cut(s) 193
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 2 cut(s) 393, 857
AfiI CCNNNNNNNGG 1 cut(s) 563
AgsI TTSAA 3 cut(s) 501, 897, 949
AjnI CCWGG 2 cut(s) 102, 406
AluBI AGCT 4 cut(s) 169, 219, 761, 786
AluI AGCT 4 cut(s) 169, 219, 761, 786
Alw21I GWGCWC 1 cut(s) 171
AlwI GGATC 5 cut(s) 94, 136, 235, 248, 307
AoxI GGCC 2 cut(s) 21, 849
ApeKI GCWGC 1 cut(s) 434
ApoI RAATTY 1 cut(s) 287
Asp700I GAANNNNTTC 1 cut(s) 402
Asp718I GGTACC 1 cut(s) 391
AspS9I GGNCC 2 cut(s) 566, 804
AsuC2I CCSGG 1 cut(s) 853
AsuHPI GGTGA 6 cut(s) 82, 176, 266, 322, 332, 919
AsuII TTCGAA 1 cut(s) 630
AvaII GGWCC 2 cut(s) 566, 804
BamHI GGATCC 1 cut(s) 240
BanI GGYRCC 1 cut(s) 391
BanII GRGCYC 1 cut(s) 171
BarI GAAGNNNNNNTAC 2 cut(s) 375, 407
BbrPI CACGTG 1 cut(s) 193
BbsI GAAGAC 2 cut(s) 238, 390
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 446
BccI CCATC 3 cut(s) 5, 127, 502
BceAI ACGGC 4 cut(s) 75, 247, 473, 541
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 2 cut(s) 104, 408
BcnI CCSGG 1 cut(s) 853
BfaI CTAG 1 cut(s) 716
BfuAI ACCTGC 1 cut(s) 538
BisI GCNGC 3 cut(s) 435, 876, 879
BlsI GCNGC 3 cut(s) 436, 877, 880
Bme1390I CCNGG 3 cut(s) 104, 408, 853
Bme18I GGWCC 2 cut(s) 566, 804
BmgT120I GGNCC 2 cut(s) 566, 804
BmiI GGNNCC 2 cut(s) 242, 393
BmrFI CCNGG 3 cut(s) 104, 408, 853
BmsI GCATC 3 cut(s) 72, 190, 543
BpiI GAAGAC 2 cut(s) 238, 390
Bpu10I CCTNAGC 2 cut(s) 86, 726
Bpu14I TTCGAA 1 cut(s) 630
BpuEI CTTGAG 1 cut(s) 671
BpuMI CCSGG 1 cut(s) 853
BsaAI YACGTR 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 6 cut(s) 103, 115, 236, 316, 423, 807
BsaWI WCCGGW 1 cut(s) 982
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 563
Bse1I ACTGG 2 cut(s) 586, 640
BseBI CCWGG 2 cut(s) 104, 408
BseDI CCNNGG 6 cut(s) 103, 115, 236, 316, 423, 807
BseGI GGATG 3 cut(s) 16, 385, 558
BseLI CCNNNNNNNGG 1 cut(s) 563
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 2 cut(s) 586, 640
BseXI GCAGC 1 cut(s) 446
BshFI GGCC 2 cut(s) 23, 851
BshNI GGYRCC 1 cut(s) 391
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 2 cut(s) 852, 983
BslI CCNNNNNNNGG 1 cut(s) 563
BsnI GGCC 2 cut(s) 23, 851
Bsp119I TTCGAA 1 cut(s) 630
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 4 cut(s) 99, 141, 240, 312
BspACI CCGC 3 cut(s) 569, 875, 878
BspANI GGCC 2 cut(s) 23, 851
BspCNI CTCAG 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 242, 393
BspMI ACCTGC 1 cut(s) 538
BspPI GGATC 5 cut(s) 94, 136, 235, 248, 307
BspT104I TTCGAA 1 cut(s) 630
BspT107I GGYRCC 1 cut(s) 391
BsrI ACTGG 2 cut(s) 586, 640
BssECI CCNNGG 6 cut(s) 103, 115, 236, 316, 423, 807
BssMI GATC 4 cut(s) 99, 141, 240, 312
BssNAI GTATAC 1 cut(s) 591
BssNI GRCGYC 1 cut(s) 363
BssT1I CCWWGG 3 cut(s) 115, 236, 316
Bst1107I GTATAC 1 cut(s) 591
Bst2UI CCWGG 2 cut(s) 104, 408
Bst4CI ACNGT 1 cut(s) 904
BstACI GRCGYC 1 cut(s) 363
BstAPI GCANNNNNTGC 2 cut(s) 463, 490
BstBAI YACGTR 1 cut(s) 193
BstBI TTCGAA 1 cut(s) 630
BstC8I GCNNGC 3 cut(s) 221, 459, 486
BstDEI CTNAG 3 cut(s) 86, 226, 726
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 3 cut(s) 16, 385, 558
BstKTI GATC 4 cut(s) 102, 144, 243, 315
BstMBI GATC 4 cut(s) 99, 141, 240, 312
BstMWI GCNNNNNNNGC 6 cut(s) 454, 463, 490, 530, 731, 872
BstNI CCWGG 2 cut(s) 104, 408
BstSCI CCNGG 3 cut(s) 102, 406, 851
BstV1I GCAGC 1 cut(s) 446
BstV2I GAAGAC 2 cut(s) 238, 390
BstX2I RGATCY 1 cut(s) 240
BstXI CCANNNNNNTGG 1 cut(s) 251
BstYI RGATCY 1 cut(s) 240
BstZ17I GTATAC 1 cut(s) 591
BsuRI GGCC 2 cut(s) 23, 851
BtgI CCRYGG 1 cut(s) 423
BtgZI GCGATG 1 cut(s) 146
BtsCI GGATG 3 cut(s) 16, 385, 558
BtsIMutI CAGTG 1 cut(s) 900
BveI ACCTGC 1 cut(s) 538
Cac8I GCNNGC 3 cut(s) 221, 459, 486
Cfr13I GGNCC 2 cut(s) 566, 804
CseI GACGC 1 cut(s) 371
Csp6I GTAC 2 cut(s) 392, 856
CspCI CAANNNNNGTGG 2 cut(s) 756, 791
CviAII CATG 7 cut(s) 34, 139, 376, 388, 463, 841, 956
CviQI GTAC 2 cut(s) 392, 856
DdeI CTNAG 3 cut(s) 86, 226, 726
DpnI GATC 4 cut(s) 101, 143, 242, 314
DpnII GATC 4 cut(s) 99, 141, 240, 312
EaeI YGGCCR 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 3 cut(s) 115, 236, 316
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 2 cut(s) 566, 804
Eco53kI GAGCTC 1 cut(s) 169
Eco72I CACGTG 1 cut(s) 193
EcoICRI GAGCTC 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 102, 406
EcoT14I CCWWGG 3 cut(s) 115, 236, 316
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 3 cut(s) 115, 236, 316
FaeI CATG 7 cut(s) 37, 142, 379, 391, 466, 844, 959
FalI AAGNNNNNCTT 2 cut(s) 196, 228
FatI CATG 7 cut(s) 33, 138, 375, 387, 462, 840, 955
FblI GTMKAC 1 cut(s) 590
Fnu4HI GCNGC 3 cut(s) 435, 876, 879
FokI GGATG 3 cut(s) 23, 392, 565
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 3 cut(s) 435, 876, 879
FspBI CTAG 1 cut(s) 716
GluI GCNGC 3 cut(s) 435, 876, 879
HaeIII GGCC 2 cut(s) 23, 851
HapII CCGG 2 cut(s) 852, 983
HgaI GACGC 1 cut(s) 371
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 7 cut(s) 37, 142, 379, 391, 466, 844, 959
HincII GTYRAC 1 cut(s) 970
HindII GTYRAC 1 cut(s) 970
HindIII AAGCTT 1 cut(s) 784
HinfI GANTC 2 cut(s) 347, 779
HpaII CCGG 2 cut(s) 852, 983
HphI GGTGA 6 cut(s) 82, 176, 266, 322, 332, 919
Hpy166II GTNNAC 3 cut(s) 267, 591, 970
Hpy188I TCNGA 3 cut(s) 146, 562, 711
Hpy188III TCNNGA 2 cut(s) 603, 688
Hpy8I GTNNAC 3 cut(s) 267, 591, 970
HpyAV CCTTC 7 cut(s) 281, 293, 301, 413, 557, 806, 883
HpyCH4III ACNGT 1 cut(s) 904
HpyCH4IV ACGT 2 cut(s) 192, 919
HpyCH4V TGCA 5 cut(s) 203, 359, 533, 698, 1006
HpyF10VI GCNNNNNNNGC 6 cut(s) 454, 463, 490, 530, 731, 872
HpyF3I CTNAG 3 cut(s) 86, 226, 726
HpySE526I ACGT 2 cut(s) 192, 919
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 7 cut(s) 37, 142, 379, 391, 466, 844, 959
KpnI GGTACC 1 cut(s) 395
Kzo9I GATC 4 cut(s) 99, 141, 240, 312
Lsp1109I GCAGC 1 cut(s) 446
LweI GCATC 3 cut(s) 72, 190, 543
MaeI CTAG 1 cut(s) 716
MaeII ACGT 2 cut(s) 192, 919
MaeIII GTNAC 3 cut(s) 320, 920, 959
MalI GATC 4 cut(s) 101, 143, 242, 314
MboI GATC 4 cut(s) 99, 141, 240, 312
MboII GAAGA 7 cut(s) 243, 395, 489, 618, 782, 861, 926
MflI RGATCY 1 cut(s) 240
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 818
MnlI CCTC 6 cut(s) 113, 406, 717, 795, 802, 935
MroXI GAANNNNTTC 1 cut(s) 402
MseI TTAA 1 cut(s) 111
MslI CAYNNNNRTG 2 cut(s) 68, 249
MspI CCGG 2 cut(s) 852, 983
MspR9I CCNGG 3 cut(s) 104, 408, 853
MvaI CCWGG 2 cut(s) 104, 408
MwoI GCNNNNNNNGC 6 cut(s) 454, 463, 490, 530, 731, 872
NciI CCSGG 1 cut(s) 853
NdeII GATC 4 cut(s) 99, 141, 240, 312
NlaIII CATG 7 cut(s) 37, 142, 379, 391, 466, 844, 959
NlaIV GGNNCC 2 cut(s) 242, 393
NmuCI GTSAC 1 cut(s) 320
NspV TTCGAA 1 cut(s) 630
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 347, 779
PfoI TCCNGGA 1 cut(s) 406
PkrI GCNGC 3 cut(s) 436, 877, 880
PmaCI CACGTG 1 cut(s) 193
PmlI CACGTG 1 cut(s) 193
Ppu21I YACGTR 1 cut(s) 193
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 102, 406
PspCI CACGTG 1 cut(s) 193
PspGI CCWGG 2 cut(s) 102, 406
PspN4I GGNNCC 2 cut(s) 242, 393
PspPI GGNCC 2 cut(s) 566, 804
PsuI RGATCY 1 cut(s) 240
RsaI GTAC 2 cut(s) 393, 857
RsaNI GTAC 2 cut(s) 392, 856
RseI CAYNNNNRTG 2 cut(s) 68, 249
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 3 cut(s) 435, 876, 879
Sau3AI GATC 4 cut(s) 99, 141, 240, 312
Sau96I GGNCC 2 cut(s) 566, 804
ScrFI CCNGG 3 cut(s) 104, 408, 853
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 3 cut(s) 72, 190, 543
SfuI TTCGAA 1 cut(s) 630
SinI GGWCC 2 cut(s) 566, 804
SmiMI CAYNNNNRTG 2 cut(s) 68, 249
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
Sse9I AATT 1 cut(s) 287
SsiI CCGC 3 cut(s) 569, 875, 878
SspMI CTAG 1 cut(s) 716
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 3 cut(s) 102, 406, 851
StyI CCWWGG 3 cut(s) 115, 236, 316
TaaI ACNGT 1 cut(s) 904
TaiI ACGT 2 cut(s) 195, 922
TaqI TCGA 3 cut(s) 339, 630, 819
TaqII GACCGA 1 cut(s) 821
TasI AATT 1 cut(s) 287
TauI GCSGC 2 cut(s) 878, 881
TfiI GAWTC 2 cut(s) 347, 779
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 1 cut(s) 907
TseFI GTSAC 1 cut(s) 320
TseI GCWGC 1 cut(s) 434
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 4 cut(s) 90, 325, 396, 742
TspGWI ACGGA 2 cut(s) 683, 978
TspRI CASTG 1 cut(s) 907
VpaK11BI GGWCC 2 cut(s) 566, 804
XapI RAATTY 1 cut(s) 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 373
XmiI GTMKAC 1 cut(s) 590
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.