pycom06g19690

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000128
Physical Location & Seq
Reverse (-)
23019397 .. 23024320
4924 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19690.3

Sequence Viewer

Length: 2748 bp
ATGGCTTCAAACAAAGAACAAGTCATGACTATCGGCACTACCTCCATTGAAAAACAGCTGGTTCAGATGAAGGAAACAATTGCAAGGCTGATAAAGACTGCAGAGGAGAAAAACTTGCAAACCGCCGGACTCATCAACCGTCTGGAGGCACAGCATGGCGTCAAAGTGAAACCAAGCTCTCTTCCAACTAAGAGGACCGAAGAAGGCTTCAACCCAAATGCCTACGAACTCATGTCAAAGGCTAGACATGACTATGCTTCCTCTTCAAATCTTGGAAAGAAGGTTTCAAACACCGTCAACGACAAAGATCGCGACCTCACCGAGACTCAAAAGAAGTTGAAGGAGCATGATTATGGAGTTGACAACAACAAGGCTGGACTTGGTTTCACACCAAATGCACCCGTGAAGATTTCAAGCAAAGTGAAGAAAGCTAGCGCTCAACACATCAGCGTGAGCATTGAACAAGACCAAGAAGAGCCTAAACCCTCCCCTCGGACGTCGGTCTTCGATAGGATGAGCCATTCAAGCTCCAGAACTTCAGTGCTTAATCGCATTGGTGGTCAAGACCGAACCCCTGTCTTCAAGAGGCTTAACGCGCCGACATCCCGAAGCTCTGTTTTTGAAAGGTTGTTAAAACCTAAGAAACAAAGCAACACAGCTATCTCTCCTCTGCGACGATCAGCTTCAGAAAGATTTGAAGATAATGGTAAGCCTTTTGGAAAGAGGAAGACAACACCAAAGGAAGAAAAGCTCGATGGGTTAGGAGAAAAAGACGACGCTCGAAGCTTGATTCCTTCAAGGATGGAGCGCCAAGCAATCCTAGAGGTCGACACAAAAGGACCACTGAAGGTAAGGAGGCGCATCATCGTCCACACTGGCAAATCTTCATGCCAACAAACCCAAGAGAACGGCACTGAAGAGGAGATCCAAGATGTTTTCTACATCACGGTTCAAGAAGGTAAAGAAGACGAAAGCCCGAGGAAATACTCAGAGTCCCTACTCTCCGACCCTGATCCAAGCGGCAGTACAACCATGCAAGTCATGACCACTGGAGCAACTTCAATCGAGGAGCAGCTAGCTCAGATGAATGAAGCAATCGCAAAGCTCACACGGACTGTGGAAGAGAAGGACTTGCAGATTGCCGCACTTGTCAACCAACTAGATGCGCTGCCCGACGTGAAAGTCGACCCACATATTGGCCTATTAAAGAAAGAAGACGACGAAGAAGAGGAGCCACCAGTGGAGAAAGTTGAAGAGAAGCCGAAGCTAGACCAAGCAACGGCATTCATGGGATCTCTCTCTATCCAGCAGCTACAGGAGATGATCGCAAGCACTGTCAAGACACAGTACGAAGGAAGCTCGCATGACTCCGCACTGTACTCAAAGCCTTACTCCAAGAAGATAGATGCTTTGAAGATGCCAAGGGGTTATCAGCCCCCGAAATTCATGCAGTTCGATGGAAAAGGCAACCCGAAGCAACATGTCGCCCATTTCATTGAAACTTGCAACAATGCTGGGACAGAGGGAGACTACCTCGTCAAACAGTTTGTGCGTTCGCTGAAAAGTAACGCTTTTGACTGGTACACTGACCTCGAACCTGAGTCTATCAACAGTTGGGACCAGCTAGAAAGGGAATTCCTCAACCGTTTCTACAATGAACCTGTCATCGACTACATAAATAGATGGCGCTCCTTAAGTCTGGATTGCAAAGATCGGCTTTCTGAAACCTCTGCCATTGAGATGTGCGTTCAAGGCATGCAGTGGGGACTACACTACATCCTTCAAGGCATCAAACCACGAACATTTGAGGAGTTAGCCACCCGCGCCCATGATATGGAGTTAAGCATCACCCGTCATGGGAAGAAAGAACAGATCGCCGACTACAAGAATGACAAAGTTTTGGGGCCCAAGGTGGATAAGGCTGCGTGGAAACCCACCAAGGAAGCAATGACGGTCAACACAACTCCAGTCAAAACCCATACACGAGGCAAGGCGATTCAAACCGAAGCTTTTCGTGATCAAGAGATACGTAGACGCACTTTGAAGGAGCTTGAGGAGAAGACTTATCCATTCCCCGACTCTGACGTGGTTGCCATGTTAGATGACTTGCTGGAAAAAAAGGTGATCAACCTACCTGAGTGTAAACGGCCGGAAGAGATGAACCGTACTGACAGTCCAAGGTACTGTAAATTCCACCGCTTCATTGGTCATCCGACGGAAAAGTGCTTTGTGCTAAAAGATCTCATCATGAAGTTGGCTCAGAAAGGGATCATCGAGCTAGATCTTAATGATGTGGTGAAATCAAACTATACCTCCGTCACTTCTGGCTCTTTAAACTCAAAATCTTCACCTCAACCGCTGGGGGCATGCTCCAAAACCATGTCAGTCAAGTCAAGTGAAGTTGAAGGATGGACTTACGTCACTCCAAAGAAAATGCACAAGAAACATAGGTCTCCTCCACAAGTTCACCAATCGGAAAGGGGGCAAAGCAGCTATCGTCAACCTTCAAAGCTACATGAAAATGTTGAGGATGATGAAGTTATGACACAAAGATCGTCCGTTGCCATCACAATGCGCGATTTCTTCCCCAAAGACTTCTTCAATCACTCAGCCACTACTTCTCCGGAGACGGTGGCGGCGACTCTCCCTCCTTCACTAGCTCCGAGTCCAGCTTCAAAGTCAACTCATCCAACGGCAGCCACAGGGACACCAGCACCACCAAGCCACTGCTCCGATCGCACGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

916

Amino Acids

103.09

Weight (kDa)

8.55

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1181, 1535
AatII GACGTC 1 cut(s) 500
AccB7I CCANNNNNTGG 2 cut(s) 1196, 1834
AccI GTMKAC 3 cut(s) 828, 1185, 2032
AccII CGCG 4 cut(s) 312, 596, 1824, 2577
AccIII TCCGGA 1 cut(s) 2623
AciI CCGC 8 cut(s) 123, 1020, 1143, 1371, 1822, 2197, 2357, 2636
AclWI GGATC 4 cut(s) 919, 1007, 1300, 2276
AcoI YGGCCR 1 cut(s) 2147
AcsI RAATTY 3 cut(s) 1442, 1634, 2189
AcuI CTGAAG 4 cut(s) 522, 669, 866, 936
AcyI GRCGYC 2 cut(s) 159, 497
AfaI GTAC 6 cut(s) 1027, 1349, 1379, 1583, 2167, 2183
AfeI AGCGCT 1 cut(s) 436
AfiI CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1834, 1857
AflII CTTAAG 1 cut(s) 1693
AflIII ACRYGT 1 cut(s) 1480
AjiI CACGTC 2 cut(s) 1177, 2086
AjuI GAANNNNNNNTTGG 2 cut(s) 2582, 2614
Alw26I GTCTC 4 cut(s) 317, 1521, 2457, 2621
AlwI GGATC 4 cut(s) 919, 1007, 1300, 2276
Ama87I CYCGRG 1 cut(s) 976
Aor13HI TCCGGA 1 cut(s) 2623
Aor51HI AGCGCT 1 cut(s) 436
AoxI GGCC 3 cut(s) 1198, 1904, 2147
ApaI GGGCCC 1 cut(s) 1908
ApeKI GCWGC 6 cut(s) 1072, 1168, 1309, 1922, 2490, 2696
ApoI RAATTY 3 cut(s) 1442, 1634, 2189
ArsI GACNNNNNNTTYG 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 2010
AspLEI GCGC 8 cut(s) 437, 598, 810, 861, 1168, 1689, 1826, 2577
AspS9I GGNCC 5 cut(s) 195, 839, 1618, 1904, 1905
AsuHPI GGTGA 6 cut(s) 310, 1840, 2134, 2308, 2340, 2459
AsuNHI GCTAGC 2 cut(s) 431, 1075
AvaI CYCGRG 1 cut(s) 976
AvaII GGWCC 3 cut(s) 195, 839, 1618
BaeGI GKGCMC 1 cut(s) 1908
BanII GRGCYC 1 cut(s) 1908
BauI CACGAG 1 cut(s) 1983
BbsI GAAGAC 6 cut(s) 496, 571, 734, 972, 1221, 2066
BbvI GCAGC 6 cut(s) 1084, 1155, 1321, 1909, 2502, 2708
BccI CCATC 6 cut(s) 749, 796, 1451, 1677, 2403, 2573
BceAI ACGGC 4 cut(s) 925, 1296, 2162, 2709
BcgI CGANNNNNNTGC 2 cut(s) 1429, 1463
BclI TGATCA 2 cut(s) 2017, 2124
BcoDI GTCTC 4 cut(s) 317, 1521, 2457, 2621
BfaI CTAG 9 cut(s) 243, 432, 821, 1076, 1160, 1268, 1625, 2279, 2657
BfmI CTRYAG 2 cut(s) 99, 1313
BfoI RGCGCY 3 cut(s) 438, 811, 1690
BfrI CTTAAG 1 cut(s) 1693
BglII AGATCT 2 cut(s) 2239, 2281
BisI GCNGC 9 cut(s) 1021, 1073, 1143, 1169, 1310, 1923, 2491, 2637, 2697
BlsI GCNGC 9 cut(s) 1022, 1074, 1144, 1170, 1311, 1924, 2492, 2638, 2698
Bme18I GGWCC 3 cut(s) 195, 839, 1618
BmeT110I CYCGRG 1 cut(s) 976
BmgBI CACGTC 2 cut(s) 1177, 2086
BmgT120I GGNCC 5 cut(s) 195, 839, 1618, 1904, 1905
BmiI GGNNCC 4 cut(s) 1233, 1619, 1905, 1906
BmsI GCATC 6 cut(s) 870, 1153, 1396, 1407, 1797, 1854
BmtI GCTAGC 2 cut(s) 435, 1079
BoxI GACNNNNGTC 2 cut(s) 500, 2417
BpiI GAAGAC 6 cut(s) 496, 571, 734, 972, 1221, 2066
BplI GAGNNNNNCTC 2 cut(s) 1518, 1550
BpmI CTGGAG 4 cut(s) 164, 514, 1071, 1950
BpuEI CTTGAG 1 cut(s) 2072
BsaAI YACGTR 1 cut(s) 2030
BsaHI GRCGYC 2 cut(s) 159, 497
BsaI GGTCTC 1 cut(s) 2457
BsaJI CCNNGG 6 cut(s) 491, 977, 1421, 1908, 1938, 2177
BsaWI WCCGGW 1 cut(s) 2623
BsaXI ACNNNNNCTCC 6 cut(s) 2297, 2327, 2605, 2632, 2635, 2662
Bsc4I CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1834, 1857
Bse1I ACTGG 5 cut(s) 880, 1054, 1238, 1583, 1967
Bse3DI GCAATG 1 cut(s) 1953
BseAI TCCGGA 1 cut(s) 2623
BseDI CCNNGG 6 cut(s) 491, 977, 1421, 1908, 1938, 2177
BseGI GGATG 8 cut(s) 519, 602, 807, 1776, 2209, 2414, 2536, 2686
BseLI CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1834, 1857
BseMI GCAATG 1 cut(s) 1953
BseMII CTCAG 6 cut(s) 1002, 1094, 1590, 2127, 2273, 2622
BseNI ACTGG 5 cut(s) 880, 1054, 1238, 1583, 1967
BseRI GAGGAG 8 cut(s) 119, 657, 935, 1082, 1244, 1823, 2069, 2445
BseSI GKGCMC 1 cut(s) 1908
BseX3I CGGCCG 1 cut(s) 2147
BseXI GCAGC 6 cut(s) 1084, 1155, 1321, 1909, 2502, 2708
BseYI CCCAGC 2 cut(s) 1514, 2359
Bsh1236I CGCG 4 cut(s) 312, 596, 1824, 2577
Bsh1285I CGRYCG 2 cut(s) 2150, 2737
BshFI GGCC 3 cut(s) 1200, 1906, 2149
BsiEI CGRYCG 2 cut(s) 2150, 2737
BsiHKCI CYCGRG 1 cut(s) 976
BsiSI CCGG 3 cut(s) 126, 2150, 2624
BslFI GGGAC 5 cut(s) 979, 1531, 1631, 1779, 2719
BslI CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1834, 1857
BsmAI GTCTC 4 cut(s) 317, 1521, 2457, 2621
BsmBI CGTCTC 1 cut(s) 2621
BsmFI GGGAC 5 cut(s) 979, 1531, 1631, 1779, 2719
BsmI GAATGC 1 cut(s) 1283
BsnI GGCC 3 cut(s) 1200, 1906, 2149
Bso31I GGTCTC 1 cut(s) 2457
BsoBI CYCGRG 1 cut(s) 976
Bsp120I GGGCCC 1 cut(s) 1904
Bsp1286I GDGCHC 1 cut(s) 1908
Bsp13I TCCGGA 1 cut(s) 2623
Bsp68I TCGCGA 1 cut(s) 312
BspACI CCGC 8 cut(s) 123, 1020, 1143, 1371, 1822, 2197, 2357, 2636
BspANI GGCC 3 cut(s) 1200, 1906, 2149
BspCNI CTCAG 6 cut(s) 1001, 1093, 1591, 2128, 2272, 2621
BspEI TCCGGA 1 cut(s) 2623
BspFNI CGCG 4 cut(s) 312, 596, 1824, 2577
BspHI TCATGA 3 cut(s) 24, 1041, 2247
BspLI GGNNCC 4 cut(s) 1233, 1619, 1905, 1906
BspMAI CTGCAG 1 cut(s) 103
BspOI GCTAGC 2 cut(s) 435, 1079
BspPI GGATC 4 cut(s) 919, 1007, 1300, 2276
BspQI GCTCTTC 1 cut(s) 468
BspTI CTTAAG 1 cut(s) 1693
BspTNI GGTCTC 1 cut(s) 2457
BsrDI GCAATG 1 cut(s) 1953
BsrI ACTGG 5 cut(s) 880, 1054, 1238, 1583, 1967
BssECI CCNNGG 6 cut(s) 491, 977, 1421, 1908, 1938, 2177
BssNI GRCGYC 2 cut(s) 159, 497
BssSI CACGAG 1 cut(s) 1983
BssT1I CCWWGG 4 cut(s) 1421, 1908, 1938, 2177
Bst2BI CACGAG 1 cut(s) 1983
Bst6I CTCTTC 8 cut(s) 186, 268, 468, 912, 1116, 1221, 1248, 2148
BstACI GRCGYC 2 cut(s) 159, 497
BstAFI CTTAAG 1 cut(s) 1693
BstBAI YACGTR 1 cut(s) 2030
BstC8I GCNNGC 6 cut(s) 433, 1077, 1330, 1361, 1757, 2368
BstDEI CTNAG 8 cut(s) 189, 639, 988, 1080, 1599, 2136, 2259, 2608
BstF5I GGATG 8 cut(s) 519, 602, 807, 1776, 2209, 2414, 2536, 2686
BstFNI CGCG 4 cut(s) 312, 596, 1824, 2577
BstH2I RGCGCY 3 cut(s) 438, 811, 1690
BstHHI GCGC 8 cut(s) 437, 598, 810, 861, 1168, 1689, 1826, 2577
BstMAI GTCTC 4 cut(s) 317, 1521, 2457, 2621
BstMCI CGRYCG 2 cut(s) 2150, 2737
BstMWI GCNNNNNNNGC 5 cut(s) 525, 595, 1752, 1823, 2736
BstNSI RCATGY 3 cut(s) 1484, 1759, 2370
BstPAI GACNNNNGTC 2 cut(s) 500, 2417
BstSFI CTRYAG 2 cut(s) 99, 1313
BstSLI GKGCMC 1 cut(s) 1908
BstSNI TACGTA 1 cut(s) 2030
BstUI CGCG 4 cut(s) 312, 596, 1824, 2577
BstV1I GCAGC 6 cut(s) 1084, 1155, 1321, 1909, 2502, 2708
BstV2I GAAGAC 6 cut(s) 496, 571, 734, 972, 1221, 2066
BstX2I RGATCY 4 cut(s) 924, 1292, 2239, 2281
BstYI RGATCY 4 cut(s) 924, 1292, 2239, 2281
BstZI CGGCCG 1 cut(s) 2147
BsuRI GGCC 3 cut(s) 1200, 1906, 2149
BtrI CACGTC 2 cut(s) 1177, 2086
BtsCI GGATG 8 cut(s) 519, 602, 807, 1776, 2209, 2414, 2536, 2686
BtsI GCAGTG 2 cut(s) 1766, 2725
BtuMI TCGCGA 1 cut(s) 312
Cac8I GCNNGC 6 cut(s) 433, 1077, 1330, 1361, 1757, 2368
CciI TCATGA 3 cut(s) 24, 1041, 2247
CfoI GCGC 8 cut(s) 437, 598, 810, 861, 1168, 1689, 1826, 2577
Cfr13I GGNCC 5 cut(s) 195, 839, 1618, 1904, 1905
CseI GACGC 3 cut(s) 148, 785, 2043
Csp6I GTAC 6 cut(s) 1026, 1348, 1378, 1582, 2166, 2182
CviQI GTAC 6 cut(s) 1026, 1348, 1378, 1582, 2166, 2182
DdeI CTNAG 8 cut(s) 189, 639, 988, 1080, 1599, 2136, 2259, 2608
DraI TTTAAA 1 cut(s) 2334
DrdI GACNNNNNNGTC 2 cut(s) 1181, 1535
DseDI GACNNNNNNGTC 2 cut(s) 1181, 1535
EaeI YGGCCR 1 cut(s) 2147
EagI CGGCCG 1 cut(s) 2147
Eam1104I CTCTTC 8 cut(s) 186, 268, 468, 912, 1116, 1221, 1248, 2148
EarI CTCTTC 8 cut(s) 186, 268, 468, 912, 1116, 1221, 1248, 2148
EclXI CGGCCG 1 cut(s) 2147
Eco105I TACGTA 1 cut(s) 2030
Eco130I CCWWGG 4 cut(s) 1421, 1908, 1938, 2177
Eco24I GRGCYC 1 cut(s) 1908
Eco31I GGTCTC 1 cut(s) 2457
Eco47I GGWCC 3 cut(s) 195, 839, 1618
Eco47III AGCGCT 1 cut(s) 436
Eco52I CGGCCG 1 cut(s) 2147
Eco57I CTGAAG 4 cut(s) 522, 669, 866, 936
Eco88I CYCGRG 1 cut(s) 976
EcoO109I RGGNCCY 1 cut(s) 1904
EcoRI GAATTC 1 cut(s) 1634
EcoT14I CCWWGG 4 cut(s) 1421, 1908, 1938, 2177
EcoT38I GRGCYC 1 cut(s) 1908
ErhI CCWWGG 4 cut(s) 1421, 1908, 1938, 2177
Esp3I CGTCTC 1 cut(s) 2621
FalI AAGNNNNNCTT 4 cut(s) 1555, 1587, 1701, 1733
FaqI GGGAC 5 cut(s) 979, 1531, 1631, 1779, 2719
FauI CCCGC 1 cut(s) 1829
FbaI TGATCA 2 cut(s) 2017, 2124
FblI GTMKAC 3 cut(s) 828, 1185, 2032
Fnu4HI GCNGC 9 cut(s) 1021, 1073, 1143, 1169, 1310, 1923, 2491, 2637, 2697
FokI GGATG 8 cut(s) 526, 589, 814, 1763, 2196, 2421, 2543, 2673
FriOI GRGCYC 1 cut(s) 1908
Fsp4HI GCNGC 9 cut(s) 1021, 1073, 1143, 1169, 1310, 1923, 2491, 2637, 2697
FspBI CTAG 9 cut(s) 243, 432, 821, 1076, 1160, 1268, 1625, 2279, 2657
GlaI GCGC 8 cut(s) 436, 597, 809, 860, 1167, 1688, 1825, 2576
GluI GCNGC 9 cut(s) 1021, 1073, 1143, 1169, 1310, 1923, 2491, 2637, 2697
GsaI CCCAGC 2 cut(s) 1518, 2363
GsuI CTGGAG 4 cut(s) 164, 514, 1071, 1950
HaeII RGCGCY 3 cut(s) 438, 811, 1690
HaeIII GGCC 3 cut(s) 1200, 1906, 2149
HapII CCGG 3 cut(s) 126, 2150, 2624
HgaI GACGC 3 cut(s) 148, 785, 2043
HhaI GCGC 8 cut(s) 437, 598, 810, 861, 1168, 1689, 1826, 2577
Hin1I GRCGYC 2 cut(s) 159, 497
Hin6I GCGC 8 cut(s) 435, 596, 808, 859, 1166, 1687, 1824, 2575
HinP1I GCGC 8 cut(s) 435, 596, 808, 859, 1166, 1687, 1824, 2575
HincII GTYRAC 8 cut(s) 298, 361, 829, 1153, 1186, 1957, 2501, 2682
HindII GTYRAC 8 cut(s) 298, 361, 829, 1153, 1186, 1957, 2501, 2682
HindIII AAGCTT 2 cut(s) 784, 2007
HpaII CCGG 3 cut(s) 126, 2150, 2624
HphI GGTGA 6 cut(s) 310, 1840, 2134, 2308, 2340, 2459
Hpy99I CGWCG 6 cut(s) 502, 678, 779, 1178, 1223, 2218
HpyCH4IV ACGT 5 cut(s) 497, 1176, 2029, 2085, 2418
HpyF10VI GCNNNNNNNGC 5 cut(s) 525, 595, 1752, 1823, 2736
HpyF3I CTNAG 8 cut(s) 189, 639, 988, 1080, 1599, 2136, 2259, 2608
HpySE526I ACGT 5 cut(s) 497, 1176, 2029, 2085, 2418
Hsp92I GRCGYC 2 cut(s) 159, 497
HspAI GCGC 8 cut(s) 435, 596, 808, 859, 1166, 1687, 1824, 2575
Kpn2I TCCGGA 1 cut(s) 2623
Ksp22I TGATCA 2 cut(s) 2017, 2124
LguI GCTCTTC 1 cut(s) 468
Lsp1109I GCAGC 6 cut(s) 1084, 1155, 1321, 1909, 2502, 2708
LweI GCATC 6 cut(s) 870, 1153, 1396, 1407, 1797, 1854
MaeI CTAG 9 cut(s) 243, 432, 821, 1076, 1160, 1268, 1625, 2279, 2657
MaeII ACGT 5 cut(s) 497, 1176, 2029, 2085, 2418
MaeIII GTNAC 3 cut(s) 1565, 2317, 2419
MfeI CAATTG 1 cut(s) 78
MflI RGATCY 4 cut(s) 924, 1292, 2239, 2281
MhlI GDGCHC 1 cut(s) 1908
MluCI AATT 4 cut(s) 78, 1442, 1634, 2189
MlyI GAGTC 8 cut(s) 123, 319, 1001, 1361, 1610, 2072, 2635, 2674
MmeI TCCRAC 4 cut(s) 209, 1029, 2237, 2714
MroI TCCGGA 1 cut(s) 2623
MroXI GAANNNNTTC 1 cut(s) 2010
MseI TTAA 8 cut(s) 546, 591, 632, 1205, 1694, 1841, 2286, 2333
MslI CAYNNNNRTG 6 cut(s) 252, 351, 449, 1739, 2520, 2570
MspA1I CMGCKG 2 cut(s) 58, 2359
MspCI CTTAAG 1 cut(s) 1693
MspI CCGG 3 cut(s) 126, 2150, 2624
MunI CAATTG 1 cut(s) 78
Mva1269I GAATGC 1 cut(s) 1283
MvnI CGCG 4 cut(s) 312, 596, 1824, 2577
MwoI GCNNNNNNNGC 5 cut(s) 525, 595, 1752, 1823, 2736
NheI GCTAGC 2 cut(s) 431, 1075
NlaIV GGNNCC 4 cut(s) 1233, 1619, 1905, 1906
NmuCI GTSAC 2 cut(s) 2317, 2419
NruI TCGCGA 1 cut(s) 312
NspI RCATGY 3 cut(s) 1484, 1759, 2370
PaeI GCATGC 2 cut(s) 1759, 2370
PagI TCATGA 3 cut(s) 24, 1041, 2247
PciI ACATGT 1 cut(s) 1480
PciSI GCTCTTC 1 cut(s) 468
PcsI WCGNNNNNNNCGW 2 cut(s) 1182, 2636
PctI GAATGC 1 cut(s) 1283
PdmI GAANNNNTTC 1 cut(s) 2010
PfeI GAWTC 2 cut(s) 790, 1996
PflMI CCANNNNNTGG 2 cut(s) 1196, 1834
PkrI GCNGC 9 cut(s) 1022, 1074, 1144, 1170, 1311, 1924, 2492, 2638, 2698
Ple19I CGATCG 1 cut(s) 2737
PleI GAGTC 8 cut(s) 123, 319, 1000, 1361, 1609, 2072, 2635, 2673
PpsI GAGTC 8 cut(s) 123, 319, 1000, 1361, 1609, 2072, 2635, 2673
Ppu21I YACGTR 1 cut(s) 2030
PscI ACATGT 1 cut(s) 1480
PshAI GACNNNNGTC 2 cut(s) 500, 2417
PspFI CCCAGC 2 cut(s) 1514, 2359
PspN4I GGNNCC 4 cut(s) 1233, 1619, 1905, 1906
PspOMI GGGCCC 1 cut(s) 1904
PspPI GGNCC 5 cut(s) 195, 839, 1618, 1904, 1905
PstI CTGCAG 1 cut(s) 103
PsuI RGATCY 4 cut(s) 924, 1292, 2239, 2281
PvuI CGATCG 1 cut(s) 2737
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 312
RsaI GTAC 6 cut(s) 1027, 1349, 1379, 1583, 2167, 2183
RsaNI GTAC 6 cut(s) 1026, 1348, 1378, 1582, 2166, 2182
RseI CAYNNNNRTG 6 cut(s) 252, 351, 449, 1739, 2520, 2570
SalI GTCGAC 2 cut(s) 827, 1184
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 8 cut(s) 546, 591, 632, 1205, 1694, 1841, 2286, 2333
SatI GCNGC 9 cut(s) 1021, 1073, 1143, 1169, 1310, 1923, 2491, 2637, 2697
Sau96I GGNCC 5 cut(s) 195, 839, 1618, 1904, 1905
SchI GAGTC 8 cut(s) 123, 319, 1001, 1361, 1610, 2072, 2635, 2674
SduI GDGCHC 1 cut(s) 1908
SfaNI GCATC 6 cut(s) 870, 1153, 1396, 1407, 1797, 1854
SfcI CTRYAG 2 cut(s) 99, 1313
SinI GGWCC 3 cut(s) 195, 839, 1618
SmiMI CAYNNNNRTG 6 cut(s) 252, 351, 449, 1739, 2520, 2570
SmlI CTYRAG 2 cut(s) 1693, 2051
SmoI CTYRAG 2 cut(s) 1693, 2051
SnaBI TACGTA 1 cut(s) 2030
SphI GCATGC 2 cut(s) 1759, 2370
Sse9I AATT 4 cut(s) 78, 1442, 1634, 2189
SsiI CCGC 8 cut(s) 123, 1020, 1143, 1371, 1822, 2197, 2357, 2636
SspMI CTAG 9 cut(s) 243, 432, 821, 1076, 1160, 1268, 1625, 2279, 2657
StyI CCWWGG 4 cut(s) 1421, 1908, 1938, 2177
TaiI ACGT 5 cut(s) 500, 1179, 2032, 2088, 2421
TaqII GACCGA 3 cut(s) 212, 490, 582
TasI AATT 4 cut(s) 78, 1442, 1634, 2189
TatI WGTACW 2 cut(s) 1025, 1377
TauI GCSGC 3 cut(s) 1023, 1145, 2639
TfiI GAWTC 2 cut(s) 790, 1996
Tru1I TTAA 8 cut(s) 546, 591, 632, 1205, 1694, 1841, 2286, 2333
Tru9I TTAA 8 cut(s) 546, 591, 632, 1205, 1694, 1841, 2286, 2333
TseFI GTSAC 2 cut(s) 2317, 2419
TseI GCWGC 6 cut(s) 1072, 1168, 1309, 1922, 2490, 2696
Tsp45I GTSAC 2 cut(s) 2317, 2419
TspGWI ACGGA 4 cut(s) 1126, 2231, 2305, 2548
Van91I CCANNNNNTGG 2 cut(s) 1196, 1834
Vha464I CTTAAG 1 cut(s) 1693
VpaK11BI GGWCC 3 cut(s) 195, 839, 1618
XapI RAATTY 3 cut(s) 1442, 1634, 2189
XceI RCATGY 3 cut(s) 1484, 1759, 2370
XcmI CCANNNNNNNNNTGG 1 cut(s) 2201
XmiI GTMKAC 3 cut(s) 828, 1185, 2032
XmnI GAANNNNTTC 1 cut(s) 2010
XspI CTAG 9 cut(s) 243, 432, 821, 1076, 1160, 1268, 1625, 2279, 2657
ZraI GACGTC 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.