pycom16g24160

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000128
Physical Location & Seq
Forward (+)
25431379 .. 25434096
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g24160.1

Sequence Viewer

Length: 2718 bp
ATGGCTTCAAACAAAGAACAAGTCATGACTATCGGCACTACCTCCATTGAAAAACAGCTGGTTCAGATGAAGGAAACAATTGCAAGGCTGATAAAGGCTGCAGAGGAGAAAAACTTGCAAACCGCCGGACTCATCAACCGTCTGGAGGCGCAACATGGTGTCATAGTGAAACCAAGCTCTCTTCCAACTAAGAGGACCGAAGAAGGCTTCAACCCAAATGCCTATGAACTCATGTCAAAGGCTGGGCATGACTTTGCTTCCTCTTCAAATCTTGGAAAGAAGGTTTCAAACACCGTCAACGACAAAGATCGCGACCTCACCGAGACTCAAAAGAAGTTGAAGGAGCATGATTATGGAGTTGACAACAACAAGGCTGGACTTGGCTTCACACCAAATGCACCCGTGAAGATTTCAAGCAAAGTGAAGAAAGCTAACGCTCAACACATCAGCGTGAGCATTGAACAAGACCAAGAAGAGCCTAAACCCTCCCCTCGGACGTCGGTCTTCGATAGGATGAGTCATTCAAGCTCCAGAACTTCAGTGCTTAATCGCATTGATGGTCAAGACCGAACCTCTGTCTTCAAGAGGCTTAACGCGCCGACATCCCAAAGCTCTGTTTTTGAAAGGTTGTTAAAGCCTAAGAAACAAAGCAACACAGCTATCTCTCCTCTGCGACGATCAGCTTCAGAAAGATTTGAAGATAATGGTAAGCATTTTGGAAAGAGGAAGACAACACTAAAGGAAGAAAAGCTCGATGGGTTAGGAGAAAAAGACGACGCTCGAAGCTTGATTCCTTCAAGGATGGAGCGCCAAGCAATCCTAGAGGTCGACACAAAAGGACCACTGAAGGTAAGGAGGCGCATCATCGTCCACACTGGCAAATCTTCATGCCAACAAACTCAAGAGAACAGCACTGAAGAGGAGATCCAAGATGTTTTCTACATCACGGTTCAAGAAGGTAAAGAAGACGAAAGCCCGAGGAAATACTCAGAGTCCCTACTCTCCGACCCTGATCCAAGCGGCAGTACAACCATGCAAGTCATGACCACTGGAGCAACTTCAATCGAGGAGCAGCTAGCTCAGATGAATGAAGCAATCGCAAAGCTCACACGGACTGTGGAAGAGAAGGACTTGCAGATTGCCGCACTTGTCAACCAACTAGATGCGCTGCCCGACGTGAAAGTCGACCCAAATATTGGCCTATTAAAGAAAGAAGACGACGAAGAAGAGGAGCCACCAGTGGAGAAAGTTGAAGAGAAGCCAAAGCTAGACCAAGCAACGGCATTCATGGGATCTTTCTCTATCCAGCAGCTACAGGAGATGATCGCAAGCACTGTCAAGACACAGTACGAAGGAAGCTCGCATGACTCCGTACTGTACTCAAAGCCTTACTCCAAGAAGATTGATGCTTTGAAGATGCCAAGGGGTTATCAGCCCCCAAAATTCATGCAGTTCGATGGAAAAGGCAACCCGAAGCAACATGTCGCCCATTTCGTTGAAACTTGCAATAATGCTGGGACAGAGGGAGACTACCTCGTCAAACAGTTCGTGCGTTCGCTGAAAAGTAACGCTTTTGACTGGTACACTGACCTCGAACCTGAGTCTATCAACAGTTGGGACCAGCTAGAAAGGGAATTCCTCAACCGTTTCTACAGCACTCGTCGTACTGTAAGCATGCTAGAGCTGACCAGTACGAAACAGTGGAGAGATGAACCTGTCATCGACTACATAAATAGATGGCGTTCATTAAGCCTGGATTGCAAAGATCGGCTTTCTGAAACCTCCGCCATCGAGATGTGCGTTCAAGGCATGCAGTGGGGACTACACTACATCCTTCAAGGCATCAAACCACGAACATTTGAGGAGTTAGCCACCCGCGCCCATGATATGGAGTTAAGCATCACCCGTCATGGGAAGAAAGAACAGATCGCCGACTACAAGAATGACAAAGTTTTGGGGCCAAAGGTGGATAAGGCTGCGTGGAAACCCACCAAGGAAGCAATGACGCTCAACACAACTCCAGTCAAAACCCATACACGAGGCAAGGCGATTCAAACCGAAGCTTTTCGTGATCAAGAGATACGTAGACGCACTTTGAAGGAGCTTGAGGAGAAGACTTATCCATTCCCCGACTCTGACGTGGTTGCCATGTTAGATGACTTGCTGGAAAAAAAGGTGATCAACCTACCTGAGTGTAAACGGCCGGAAGAGATGAACCGTACTGACAGTCCAAGGTACTGTAAATTCCACCGCTTCATTGGTCATCCGACGGAAAAGTGCTTTGTGCTAAAAGATCTCATCATGAAGTTGGCTCAGAAAGGGATCATCGAGCTAGATCTTGATGATGTGGTGAAGTCAAACTATACCACCGTCACTTCTGGCTCTTTGAACTCAAAATCTTCACCTCAACCGCTGGGGGCATGCTCCAAAACCATGTCAGTCAAGTCAAGTGAAGATGAAGGATGGATTCATGTCACTCCAAAGAAAATGTACAAGAAACATAGGTCTCCTCCACAAGTTCACCAATCGGAAAGGGGGCAAAGCAGCTACCGTCAACCTTCAAAGCTACATGAAAGTGTTGAGGATGATGAAGTTATGACACAAAGATCGTCCGTTGCCATCACGATGCGCGATTTCTTCCCAAAAGACTTCTTCAATCACTCAGTCAAGACTCCTTGCTATAAAGATTGCAAGGAATGCCTTTCTAAGATCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

906

Amino Acids

102.87

Weight (kDa)

8.32

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 516 - 605 6e-13 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1181, 1535
AatII GACGTC 1 cut(s) 500
AccB7I CCANNNNNTGG 2 cut(s) 1196, 1888
AccI GTMKAC 3 cut(s) 828, 1185, 2086
AccII CGCG 4 cut(s) 312, 596, 1878, 2631
AciI CCGC 7 cut(s) 123, 1020, 1143, 1785, 1876, 2251, 2411
AclWI GGATC 4 cut(s) 919, 1007, 1300, 2330
AcoI YGGCCR 1 cut(s) 2201
AcsI RAATTY 3 cut(s) 1442, 1634, 2243
AcuI CTGAAG 4 cut(s) 522, 669, 866, 936
AcyI GRCGYC 1 cut(s) 497
AfiI CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1888, 1911
AflIII ACRYGT 1 cut(s) 1480
AjiI CACGTC 2 cut(s) 1177, 2140
AjnI CCWGG 1 cut(s) 1752
Alw26I GTCTC 3 cut(s) 317, 1521, 2511
AlwI GGATC 4 cut(s) 919, 1007, 1300, 2330
Ama87I CYCGRG 1 cut(s) 976
AoxI GGCC 3 cut(s) 1198, 1958, 2201
ApeKI GCWGC 6 cut(s) 98, 1072, 1168, 1309, 1976, 2544
ApoI RAATTY 3 cut(s) 1442, 1634, 2243
ArsI GACNNNNNNTTYG 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 2064
AspLEI GCGC 7 cut(s) 151, 598, 810, 861, 1168, 1880, 2631
AspS9I GGNCC 4 cut(s) 195, 839, 1618, 1958
AsuHPI GGTGA 6 cut(s) 310, 1894, 2188, 2362, 2394, 2513
AsuNHI GCTAGC 1 cut(s) 1075
AvaI CYCGRG 1 cut(s) 976
AvaII GGWCC 3 cut(s) 195, 839, 1618
BauI CACGAG 1 cut(s) 2037
BbsI GAAGAC 6 cut(s) 496, 571, 734, 972, 1221, 2120
BbvI GCAGC 6 cut(s) 85, 1084, 1155, 1321, 1963, 2556
BccI CCATC 8 cut(s) 551, 749, 796, 1451, 1731, 1796, 2457, 2627
BceAI ACGGC 2 cut(s) 1296, 2216
BciT130I CCWGG 1 cut(s) 1754
BclI TGATCA 2 cut(s) 2071, 2178
BcoDI GTCTC 3 cut(s) 317, 1521, 2511
BfaI CTAG 7 cut(s) 821, 1076, 1160, 1268, 1625, 1679, 2333
BfmI CTRYAG 3 cut(s) 99, 1313, 1651
BfoI RGCGCY 1 cut(s) 811
BglII AGATCT 2 cut(s) 2293, 2335
BisI GCNGC 8 cut(s) 99, 1021, 1073, 1143, 1169, 1310, 1977, 2545
BlsI GCNGC 8 cut(s) 100, 1022, 1074, 1144, 1170, 1311, 1978, 2546
Bme1390I CCNGG 1 cut(s) 1754
Bme18I GGWCC 3 cut(s) 195, 839, 1618
BmeT110I CYCGRG 1 cut(s) 976
BmgBI CACGTC 2 cut(s) 1177, 2140
BmgT120I GGNCC 4 cut(s) 195, 839, 1618, 1958
BmiI GGNNCC 3 cut(s) 1233, 1619, 1959
BmrFI CCNGG 1 cut(s) 1754
BmsI GCATC 7 cut(s) 870, 1153, 1396, 1407, 1851, 1908, 2616
BmtI GCTAGC 1 cut(s) 1079
BoxI GACNNNNGTC 1 cut(s) 500
BpiI GAAGAC 6 cut(s) 496, 571, 734, 972, 1221, 2120
BplI GAGNNNNNCTC 2 cut(s) 1518, 1550
BpmI CTGGAG 4 cut(s) 164, 514, 1071, 2004
BpuEI CTTGAG 2 cut(s) 885, 2126
BsaAI YACGTR 1 cut(s) 2084
BsaHI GRCGYC 1 cut(s) 497
BsaI GGTCTC 1 cut(s) 2511
BsaJI CCNNGG 5 cut(s) 491, 977, 1421, 1992, 2231
Bsc4I CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1888, 1911
Bse1I ACTGG 6 cut(s) 880, 1054, 1238, 1583, 1689, 2021
Bse3DI GCAATG 1 cut(s) 2007
BseBI CCWGG 1 cut(s) 1754
BseDI CCNNGG 5 cut(s) 491, 977, 1421, 1992, 2231
BseGI GGATG 7 cut(s) 519, 602, 807, 1830, 2263, 2468, 2590
BseLI CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1888, 1911
BseMI GCAATG 1 cut(s) 2007
BseMII CTCAG 6 cut(s) 1002, 1094, 1590, 2181, 2327, 2676
BseNI ACTGG 6 cut(s) 880, 1054, 1238, 1583, 1689, 2021
BseRI GAGGAG 8 cut(s) 119, 657, 935, 1082, 1244, 1877, 2123, 2499
BseX3I CGGCCG 1 cut(s) 2201
BseXI GCAGC 6 cut(s) 85, 1084, 1155, 1321, 1963, 2556
BseYI CCCAGC 3 cut(s) 242, 1514, 2413
Bsh1236I CGCG 4 cut(s) 312, 596, 1878, 2631
Bsh1285I CGRYCG 1 cut(s) 2204
BshFI GGCC 3 cut(s) 1200, 1960, 2203
BsiEI CGRYCG 1 cut(s) 2204
BsiHKCI CYCGRG 1 cut(s) 976
BsiSI CCGG 2 cut(s) 126, 2204
BslFI GGGAC 4 cut(s) 979, 1531, 1631, 1833
BslI CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1888, 1911
BsmAI GTCTC 3 cut(s) 317, 1521, 2511
BsmFI GGGAC 4 cut(s) 979, 1531, 1631, 1833
BsmI GAATGC 2 cut(s) 1283, 2702
BsnI GGCC 3 cut(s) 1200, 1960, 2203
Bso31I GGTCTC 1 cut(s) 2511
BsoBI CYCGRG 1 cut(s) 976
Bsp1407I TGTACA 1 cut(s) 2490
Bsp68I TCGCGA 1 cut(s) 312
BspACI CCGC 7 cut(s) 123, 1020, 1143, 1785, 1876, 2251, 2411
BspANI GGCC 3 cut(s) 1200, 1960, 2203
BspCNI CTCAG 6 cut(s) 1001, 1093, 1591, 2182, 2326, 2675
BspFNI CGCG 4 cut(s) 312, 596, 1878, 2631
BspHI TCATGA 3 cut(s) 24, 1041, 2301
BspLI GGNNCC 3 cut(s) 1233, 1619, 1959
BspMAI CTGCAG 1 cut(s) 103
BspOI GCTAGC 1 cut(s) 1079
BspPI GGATC 4 cut(s) 919, 1007, 1300, 2330
BspQI GCTCTTC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 2511
BsrDI GCAATG 1 cut(s) 2007
BsrGI TGTACA 1 cut(s) 2490
BsrI ACTGG 6 cut(s) 880, 1054, 1238, 1583, 1689, 2021
BssECI CCNNGG 5 cut(s) 491, 977, 1421, 1992, 2231
BssNI GRCGYC 1 cut(s) 497
BssSI CACGAG 1 cut(s) 2037
BssT1I CCWWGG 3 cut(s) 1421, 1992, 2231
Bst2BI CACGAG 1 cut(s) 2037
Bst2UI CCWGG 1 cut(s) 1754
Bst6I CTCTTC 8 cut(s) 186, 268, 468, 912, 1116, 1221, 1248, 2202
BstACI GRCGYC 1 cut(s) 497
BstAPI GCANNNNNTGC 1 cut(s) 2697
BstAUI TGTACA 1 cut(s) 2490
BstBAI YACGTR 1 cut(s) 2084
BstC8I GCNNGC 6 cut(s) 1077, 1330, 1361, 1676, 1811, 2422
BstDEI CTNAG 9 cut(s) 189, 639, 988, 1080, 1599, 2190, 2313, 2662, 2706
BstF5I GGATG 7 cut(s) 519, 602, 807, 1830, 2263, 2468, 2590
BstFNI CGCG 4 cut(s) 312, 596, 1878, 2631
BstH2I RGCGCY 1 cut(s) 811
BstHHI GCGC 7 cut(s) 151, 598, 810, 861, 1168, 1880, 2631
BstMAI GTCTC 3 cut(s) 317, 1521, 2511
BstMCI CGRYCG 1 cut(s) 2204
BstMWI GCNNNNNNNGC 5 cut(s) 595, 1758, 1806, 1877, 2697
BstNI CCWGG 1 cut(s) 1754
BstNSI RCATGY 4 cut(s) 1484, 1678, 1813, 2424
BstPAI GACNNNNGTC 1 cut(s) 500
BstSCI CCNGG 1 cut(s) 1752
BstSFI CTRYAG 3 cut(s) 99, 1313, 1651
BstSNI TACGTA 1 cut(s) 2084
BstUI CGCG 4 cut(s) 312, 596, 1878, 2631
BstV1I GCAGC 6 cut(s) 85, 1084, 1155, 1321, 1963, 2556
BstV2I GAAGAC 6 cut(s) 496, 571, 734, 972, 1221, 2120
BstX2I RGATCY 4 cut(s) 924, 1292, 2293, 2335
BstYI RGATCY 4 cut(s) 924, 1292, 2293, 2335
BstZI CGGCCG 1 cut(s) 2201
BsuRI GGCC 3 cut(s) 1200, 1960, 2203
BtrI CACGTC 2 cut(s) 1177, 2140
BtsCI GGATG 7 cut(s) 519, 602, 807, 1830, 2263, 2468, 2590
BtsI GCAGTG 1 cut(s) 1820
BtuMI TCGCGA 1 cut(s) 312
Cac8I GCNNGC 6 cut(s) 1077, 1330, 1361, 1676, 1811, 2422
CciI TCATGA 3 cut(s) 24, 1041, 2301
CfoI GCGC 7 cut(s) 151, 598, 810, 861, 1168, 1880, 2631
Cfr13I GGNCC 4 cut(s) 195, 839, 1618, 1958
CseI GACGC 3 cut(s) 785, 2014, 2097
DdeI CTNAG 9 cut(s) 189, 639, 988, 1080, 1599, 2190, 2313, 2662, 2706
DrdI GACNNNNNNGTC 2 cut(s) 1181, 1535
DseDI GACNNNNNNGTC 2 cut(s) 1181, 1535
EaeI YGGCCR 1 cut(s) 2201
EagI CGGCCG 1 cut(s) 2201
Eam1104I CTCTTC 8 cut(s) 186, 268, 468, 912, 1116, 1221, 1248, 2202
EarI CTCTTC 8 cut(s) 186, 268, 468, 912, 1116, 1221, 1248, 2202
EciI GGCGGA 1 cut(s) 1774
EclXI CGGCCG 1 cut(s) 2201
Eco105I TACGTA 1 cut(s) 2084
Eco130I CCWWGG 3 cut(s) 1421, 1992, 2231
Eco31I GGTCTC 1 cut(s) 2511
Eco47I GGWCC 3 cut(s) 195, 839, 1618
Eco52I CGGCCG 1 cut(s) 2201
Eco57I CTGAAG 4 cut(s) 522, 669, 866, 936
Eco88I CYCGRG 1 cut(s) 976
EcoRI GAATTC 1 cut(s) 1634
EcoRII CCWGG 1 cut(s) 1752
EcoT14I CCWWGG 3 cut(s) 1421, 1992, 2231
ErhI CCWWGG 3 cut(s) 1421, 1992, 2231
FalI AAGNNNNNCTT 4 cut(s) 1555, 1587, 1755, 1787
FaqI GGGAC 4 cut(s) 979, 1531, 1631, 1833
FauI CCCGC 1 cut(s) 1883
FbaI TGATCA 2 cut(s) 2071, 2178
FblI GTMKAC 3 cut(s) 828, 1185, 2086
Fnu4HI GCNGC 8 cut(s) 99, 1021, 1073, 1143, 1169, 1310, 1977, 2545
FokI GGATG 7 cut(s) 526, 589, 814, 1817, 2250, 2475, 2597
Fsp4HI GCNGC 8 cut(s) 99, 1021, 1073, 1143, 1169, 1310, 1977, 2545
FspBI CTAG 7 cut(s) 821, 1076, 1160, 1268, 1625, 1679, 2333
GlaI GCGC 7 cut(s) 150, 597, 809, 860, 1167, 1879, 2630
GluI GCNGC 8 cut(s) 99, 1021, 1073, 1143, 1169, 1310, 1977, 2545
GsaI CCCAGC 3 cut(s) 246, 1518, 2417
GsuI CTGGAG 4 cut(s) 164, 514, 1071, 2004
HaeII RGCGCY 1 cut(s) 811
HaeIII GGCC 3 cut(s) 1200, 1960, 2203
HapII CCGG 2 cut(s) 126, 2204
HgaI GACGC 3 cut(s) 785, 2014, 2097
HhaI GCGC 7 cut(s) 151, 598, 810, 861, 1168, 1880, 2631
Hin1I GRCGYC 1 cut(s) 497
Hin6I GCGC 7 cut(s) 149, 596, 808, 859, 1166, 1878, 2629
HinP1I GCGC 7 cut(s) 149, 596, 808, 859, 1166, 1878, 2629
HincII GTYRAC 6 cut(s) 298, 361, 829, 1153, 1186, 2555
HindII GTYRAC 6 cut(s) 298, 361, 829, 1153, 1186, 2555
HindIII AAGCTT 2 cut(s) 784, 2061
HpaII CCGG 2 cut(s) 126, 2204
HphI GGTGA 6 cut(s) 310, 1894, 2188, 2362, 2394, 2513
Hpy99I CGWCG 7 cut(s) 502, 678, 779, 1178, 1223, 1665, 2272
HpyCH4IV ACGT 4 cut(s) 497, 1176, 2083, 2139
HpyF10VI GCNNNNNNNGC 5 cut(s) 595, 1758, 1806, 1877, 2697
HpyF3I CTNAG 9 cut(s) 189, 639, 988, 1080, 1599, 2190, 2313, 2662, 2706
HpySE526I ACGT 4 cut(s) 497, 1176, 2083, 2139
Hsp92I GRCGYC 1 cut(s) 497
HspAI GCGC 7 cut(s) 149, 596, 808, 859, 1166, 1878, 2629
Ksp22I TGATCA 2 cut(s) 2071, 2178
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 8 cut(s) 343, 533, 805, 1052, 1069, 1231, 2101, 2429
Lsp1109I GCAGC 6 cut(s) 85, 1084, 1155, 1321, 1963, 2556
LweI GCATC 7 cut(s) 870, 1153, 1396, 1407, 1851, 1908, 2616
MaeI CTAG 7 cut(s) 821, 1076, 1160, 1268, 1625, 1679, 2333
MaeII ACGT 4 cut(s) 497, 1176, 2083, 2139
MaeIII GTNAC 3 cut(s) 1565, 2371, 2473
MfeI CAATTG 1 cut(s) 78
MflI RGATCY 4 cut(s) 924, 1292, 2293, 2335
MluCI AATT 4 cut(s) 78, 1442, 1634, 2243
MlyI GAGTC 8 cut(s) 123, 319, 526, 1001, 1361, 1610, 2126, 2665
MmeI TCCRAC 3 cut(s) 209, 1029, 2291
MroXI GAANNNNTTC 1 cut(s) 2064
MseI TTAA 6 cut(s) 546, 591, 632, 1205, 1748, 1895
MslI CAYNNNNRTG 5 cut(s) 351, 449, 1793, 2574, 2624
MspA1I CMGCKG 2 cut(s) 58, 2413
MspI CCGG 2 cut(s) 126, 2204
MspR9I CCNGG 1 cut(s) 1754
MunI CAATTG 1 cut(s) 78
Mva1269I GAATGC 2 cut(s) 1283, 2702
MvaI CCWGG 1 cut(s) 1754
MvnI CGCG 4 cut(s) 312, 596, 1878, 2631
MwoI GCNNNNNNNGC 5 cut(s) 595, 1758, 1806, 1877, 2697
NheI GCTAGC 1 cut(s) 1075
NlaIV GGNNCC 3 cut(s) 1233, 1619, 1959
NmuCI GTSAC 2 cut(s) 2371, 2473
NruI TCGCGA 1 cut(s) 312
NspI RCATGY 4 cut(s) 1484, 1678, 1813, 2424
PaeI GCATGC 3 cut(s) 1678, 1813, 2424
PagI TCATGA 3 cut(s) 24, 1041, 2301
PciI ACATGT 1 cut(s) 1480
PciSI GCTCTTC 1 cut(s) 468
PcsI WCGNNNNNNNCGW 3 cut(s) 1182, 1491, 1797
PctI GAATGC 2 cut(s) 1283, 2702
PdmI GAANNNNTTC 1 cut(s) 2064
PfeI GAWTC 3 cut(s) 790, 2050, 2467
PflMI CCANNNNNTGG 2 cut(s) 1196, 1888
PkrI GCNGC 8 cut(s) 100, 1022, 1074, 1144, 1170, 1311, 1978, 2546
PleI GAGTC 8 cut(s) 123, 319, 525, 1000, 1361, 1609, 2126, 2665
PpsI GAGTC 8 cut(s) 123, 319, 525, 1000, 1361, 1609, 2126, 2665
Ppu21I YACGTR 1 cut(s) 2084
PscI ACATGT 1 cut(s) 1480
PshAI GACNNNNGTC 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 1752
PspFI CCCAGC 3 cut(s) 242, 1514, 2413
PspGI CCWGG 1 cut(s) 1752
PspN4I GGNNCC 3 cut(s) 1233, 1619, 1959
PspPI GGNCC 4 cut(s) 195, 839, 1618, 1958
PstI CTGCAG 1 cut(s) 103
PsuI RGATCY 4 cut(s) 924, 1292, 2293, 2335
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 312
RseI CAYNNNNRTG 5 cut(s) 351, 449, 1793, 2574, 2624
SalI GTCGAC 2 cut(s) 827, 1184
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 6 cut(s) 546, 591, 632, 1205, 1748, 1895
SatI GCNGC 8 cut(s) 99, 1021, 1073, 1143, 1169, 1310, 1977, 2545
Sau96I GGNCC 4 cut(s) 195, 839, 1618, 1958
SchI GAGTC 8 cut(s) 123, 319, 526, 1001, 1361, 1610, 2126, 2665
ScrFI CCNGG 1 cut(s) 1754
SfaNI GCATC 7 cut(s) 870, 1153, 1396, 1407, 1851, 1908, 2616
SfcI CTRYAG 3 cut(s) 99, 1313, 1651
SinI GGWCC 3 cut(s) 195, 839, 1618
SmiMI CAYNNNNRTG 5 cut(s) 351, 449, 1793, 2574, 2624
SmlI CTYRAG 2 cut(s) 900, 2105
SmoI CTYRAG 2 cut(s) 900, 2105
SnaBI TACGTA 1 cut(s) 2084
SphI GCATGC 3 cut(s) 1678, 1813, 2424
Sse9I AATT 4 cut(s) 78, 1442, 1634, 2243
SsiI CCGC 7 cut(s) 123, 1020, 1143, 1785, 1876, 2251, 2411
SspI AATATT 1 cut(s) 1195
SspMI CTAG 7 cut(s) 821, 1076, 1160, 1268, 1625, 1679, 2333
StyD4I CCNGG 1 cut(s) 1752
StyI CCWWGG 3 cut(s) 1421, 1992, 2231
TaiI ACGT 4 cut(s) 500, 1179, 2086, 2142
TaqII GACCGA 3 cut(s) 212, 490, 582
TasI AATT 4 cut(s) 78, 1442, 1634, 2243
TatI WGTACW 3 cut(s) 1025, 1377, 2490
TauI GCSGC 2 cut(s) 1023, 1145
TfiI GAWTC 3 cut(s) 790, 2050, 2467
Tru1I TTAA 6 cut(s) 546, 591, 632, 1205, 1748, 1895
Tru9I TTAA 6 cut(s) 546, 591, 632, 1205, 1748, 1895
TseFI GTSAC 2 cut(s) 2371, 2473
TseI GCWGC 6 cut(s) 98, 1072, 1168, 1309, 1976, 2544
Tsp45I GTSAC 2 cut(s) 2371, 2473
TspGWI ACGGA 4 cut(s) 1126, 1360, 2285, 2602
Van91I CCANNNNNTGG 2 cut(s) 1196, 1888
VpaK11BI GGWCC 3 cut(s) 195, 839, 1618
XapI RAATTY 3 cut(s) 1442, 1634, 2243
XceI RCATGY 4 cut(s) 1484, 1678, 1813, 2424
XcmI CCANNNNNNNNNTGG 1 cut(s) 2255
XmiI GTMKAC 3 cut(s) 828, 1185, 2086
XmnI GAANNNNTTC 1 cut(s) 2064
XspI CTAG 7 cut(s) 821, 1076, 1160, 1268, 1625, 1679, 2333
ZraI GACGTC 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.