pycom576g00060

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000576
OG0000128
Physical Location & Seq
Forward (+)
51198 .. 53360
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom576g00060.1

Sequence Viewer

Length: 2163 bp
ATGGCATCAAGAAAGGATCAAGCTGTTCCTGCAATCAAAGCAAAGAACAAGAACATCATCGCCGCAAGTGGTGATATTTCGGGCATCATAACCCGAAGTAAGGCAAGAGCTCTTTTTGCCGCGGCTTCCGCCCCTGCATTGACTCTGTCAAAGGAACAAGAGCACCCGAGGTACGAGCCTGTGATCACCCTAGCCTCGCTAAGGGCATCAAGGGGGGAAAGCCCGAGAAAATACTCAGAGTCCCTACTCTTCGACGCTGATTCGAGCGGCAGTACAACCATGCAAGTCATGACCACTGGAGCAACTTCAATCCAGGAGCAGCTAGCTCAGATGAATGAAGCAATCGCAAAGCTCACACGGACTGTGGAAGAGAAGGACTTGCAGATTGCCGCACTTGTCAACCAACTAGATGTGCTGCCCGACATGAAAGTCGACCCAAATGTTGGCCTATTAAAGAAAGAAGTCGACGAAGAAGAGGAGCCACCAGCGGAGAAAGTTGAAGAGAAGTCGAAGCTAGACCAAGCAACGACATTCATGGGATCTCTCTCTATCCAGCAGCTACAGGAGATGATCGCAAGCACTGTCAAGACACAGTACGAAGGAAGCTCGCATGACTCCGTACTGTACTCAAAGCCTTACTCCAAGAAGATTGATGCTTTGAAGATGCCAAAGGGTTATCAGCCCTCAAAATTCATACAGTTCGATGGAAAAGGCAACCCGAAGCAACATGTCGCCCATTTCATTGAAACTTGCAACAATGCTGGGACAGAGGGAGACTACCTCATCAAACAATTCATGCGTTCGCTGAAAAGTAACGCTTTTGACTGGTACACTGACCTCGAACCTGAGTCTATCAACAGTTGGGACCAGCTAGAAAGGGAATTCCTCAACCGTTTCTACAGCACTCGTCGTACTGTAAGCATGCTAGAGCTGACCAGTACGAAACAGTGGAGAGATGAACCTGTCGTCGACAACATAAATAGATGGCACTCCTTAAGTCTGGATTGTAAAGATCGGCTTTCTGAAACCTCTGCCATTGAGATGTGCGTTCAAGGCATGCACTGGGGGTTACATTACATCCTCCAAGGCATAAAACCAAGAACATTCGAAGAGCTAGCAACTCGTTCCCACGACATGGAGCTGAGTATCGCTAATCACGAGAAAAAGGAGCCGATTACTAACTTCAATAAGGATAAAGTATTCGCCCCAAATGTAGACAAGACCGGGAAGAAGCCCGCTAAGGAAGCTTTCACCGTCGACACTACCCCCATCAAGACCTCCTCAGCGCCTATCAAGACCTCATCCAAGAAGAAAGCGAAGGAGATAAAGACAAGTGAGCCTTCTCGCACCCAAGACAGATACAAGAGCACCTTGAGAGAGTTGGAACAAAAAACGTACCCTTTTCCTGACTCTGACATGGCTGCCATGTTAGATGACTTGCTGGAAAAAAAGGTGATTAAGCTACCCGAATGCAAGCGTCCTGAAGAAATGAATCGCACAAATGATCCTAGGTACTGCAAGTACCATCGTATTGTGAGCCATCCTGTTGGCAAGTGCTTCGTTCTCAAAGAACTCATCATGAAGCTAGCACAACAAGGGCAAATTGAGCTCGACCTTGAGGACACGGCCGCAACGCATACCACTACAATTGCGTTTGGATCGTTTAATCCTGTGCCTCTCCAAACGACACCTGACCGTTCCCGTGAATGCTCAAGTTGCACGGCACCTCCTACACAACCATCACTGGAGAGAAGCGACCAAGATGCACATGCTGATGATGAAGAAGGATGGATATTGGTAACCTACAAAAAGACAAGGAAACCAAGACCACGAGTCATACAGCCAAATGTGGAACAAAGAAGAAATCACCACGGCCGCAACAATGCGAAGCCTAAAAGAAATATAAAGCATAATAAAACAACATATGCTAGGGAACCTATGGAGCAGGAGCCACACATTCCCGTTTCCTTGCGTGAGTACTTCCCGAATGACTTTTTCCAACAGTGCACTACCGTGGCCTGTCACATGGTTGAAGTAGAAATAGAAGAGCCCTCAAAAGGCAAAGCTATCGCCATTGAGGTAGAAAAGACCCTCACACCTGAAGAAGGTCTGCCAACAGACTTTAGCATCGAGGAAGCGCTACCATTGCCAAAAAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

721

Amino Acids

81.57

Weight (kDa)

6.84

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 266 - 354 2.5e-11 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 428
AccB1I GGYRCC 1 cut(s) 1723
AccB7I CCANNNNNTGG 2 cut(s) 443, 1135
AccBSI CCGCTC 1 cut(s) 267
AccI GTMKAC 5 cut(s) 432, 465, 969, 1215, 1257
AccII CGCG 1 cut(s) 122
AclWI GGATC 4 cut(s) 24, 547, 1499, 1666
AcoI YGGCCR 2 cut(s) 1626, 1873
AcsI RAATTY 2 cut(s) 689, 881
AcuI CTGAAG 2 cut(s) 1503, 2121
AfeI AGCGCT 1 cut(s) 2139
AfiI CCNNNNNNNGG 6 cut(s) 100, 201, 443, 1135, 2057, 2105
AflII CTTAAG 1 cut(s) 994
AflIII ACRYGT 1 cut(s) 727
AgsI TTSAA 7 cut(s) 309, 500, 661, 746, 1052, 1186, 2033
AhdI GACNNNNNGTC 1 cut(s) 1832
AjnI CCWGG 1 cut(s) 312
AleI CACNNNNGTG 1 cut(s) 2012
Alw21I GWGCWC 5 cut(s) 112, 165, 1370, 1611, 2009
Alw26I GTCTC 1 cut(s) 768
Alw44I GTGCAC 1 cut(s) 2005
AlwI GGATC 4 cut(s) 24, 547, 1499, 1666
Ama87I CYCGRG 2 cut(s) 166, 223
Aor51HI AGCGCT 1 cut(s) 2139
AoxI GGCC 4 cut(s) 445, 1626, 1873, 2016
ApaLI GTGCAC 1 cut(s) 2005
ApeKI GCWGC 4 cut(s) 319, 415, 556, 1421
ApoI RAATTY 2 cut(s) 689, 881
AspA2I CCTAGG 1 cut(s) 1508
AspLEI GCGC 2 cut(s) 1288, 2140
AspS9I GGNCC 1 cut(s) 865
AsuC2I CCSGG 1 cut(s) 1225
AsuHPI GGTGA 5 cut(s) 83, 178, 1243, 1465, 1859
AsuII TTCGAA 1 cut(s) 1107
AsuNHI GCTAGC 3 cut(s) 322, 1114, 1585
AvaI CYCGRG 2 cut(s) 166, 223
AvaII GGWCC 1 cut(s) 865
AvrII CCTAGG 1 cut(s) 1508
BaeGI GKGCMC 1 cut(s) 2009
BanI GGYRCC 1 cut(s) 1723
BanII GRGCYC 3 cut(s) 112, 1611, 2052
BauI CACGAG 2 cut(s) 1157, 1830
Bbv12I GWGCWC 5 cut(s) 112, 165, 1370, 1611, 2009
BbvCI CCTCAGC 1 cut(s) 1282
BbvI GCAGC 4 cut(s) 331, 402, 568, 1408
BccI CCATC 7 cut(s) 698, 978, 1277, 1533, 1548, 1747, 1782
BceAI ACGGC 3 cut(s) 1641, 1737, 1888
BcgI CGANNNNNNTGC 6 cut(s) 1540, 1574, 1745, 1779, 2050, 2084
BciT130I CCWGG 1 cut(s) 314
BclI TGATCA 1 cut(s) 183
BcnI CCSGG 1 cut(s) 1225
BcoDI GTCTC 1 cut(s) 768
BfmI CTRYAG 2 cut(s) 560, 898
BfoI RGCGCY 2 cut(s) 1289, 2141
BfrI CTTAAG 1 cut(s) 994
BlnI CCTAGG 1 cut(s) 1508
BmcAI AGTACT 1 cut(s) 1979
Bme1390I CCNGG 2 cut(s) 314, 1225
Bme18I GGWCC 1 cut(s) 865
BmeRI GACNNNNNGTC 1 cut(s) 1832
BmeT110I CYCGRG 2 cut(s) 166, 223
BmgT120I GGNCC 1 cut(s) 865
BmiI GGNNCC 6 cut(s) 480, 866, 1170, 1725, 1935, 1950
BmrFI CCNGG 2 cut(s) 314, 1225
BmrI ACTGGG 1 cut(s) 1072
BmsI GCATC 7 cut(s) 14, 93, 215, 643, 654, 1753, 2136
BmtI GCTAGC 3 cut(s) 326, 1118, 1589
BmuI ACTGGG 1 cut(s) 1072
BplI GAGNNNNNCTC 2 cut(s) 765, 797
BpmI CTGGAG 2 cut(s) 318, 1766
Bpu10I CCTNAGC 3 cut(s) 200, 1239, 1282
Bpu14I TTCGAA 1 cut(s) 1107
BpuEI CTTGAG 3 cut(s) 1393, 1637, 1696
BpuMI CCSGG 1 cut(s) 1225
BsaJI CCNNGG 6 cut(s) 120, 167, 1084, 1508, 1870, 2013
BsaXI ACNNNNNCTCC 2 cut(s) 1711, 1741
Bsc4I CCNNNNNNNGG 6 cut(s) 100, 201, 443, 1135, 2057, 2105
Bse1I ACTGG 5 cut(s) 301, 830, 936, 1067, 1749
Bse3DI GCAATG 1 cut(s) 2144
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 6 cut(s) 120, 167, 1084, 1508, 1870, 2013
BseGI GGATG 4 cut(s) 1077, 1301, 1540, 1793
BseLI CCNNNNNNNGG 6 cut(s) 100, 201, 443, 1135, 2057, 2105
BseMI GCAATG 1 cut(s) 2144
BseMII CTCAG 5 cut(s) 249, 341, 837, 1133, 1296
BseNI ACTGG 5 cut(s) 301, 830, 936, 1067, 1749
BseRI GAGGAG 2 cut(s) 491, 1270
BseSI GKGCMC 1 cut(s) 2009
BseX3I CGGCCG 2 cut(s) 1626, 1873
BseXI GCAGC 4 cut(s) 331, 402, 568, 1408
BseYI CCCAGC 1 cut(s) 761
Bsh1236I CGCG 1 cut(s) 122
Bsh1285I CGRYCG 2 cut(s) 1629, 1876
BshFI GGCC 4 cut(s) 447, 1628, 1875, 2018
BshNI GGYRCC 1 cut(s) 1723
BsiEI CGRYCG 2 cut(s) 1629, 1876
BsiHKAI GWGCWC 5 cut(s) 112, 165, 1370, 1611, 2009
BsiHKCI CYCGRG 2 cut(s) 166, 223
BsiSI CCGG 1 cut(s) 1224
BslFI GGGAC 3 cut(s) 226, 778, 878
BslI CCNNNNNNNGG 6 cut(s) 100, 201, 443, 1135, 2057, 2105
BsmAI GTCTC 1 cut(s) 768
BsmFI GGGAC 3 cut(s) 226, 778, 878
BsmI GAATGC 2 cut(s) 1475, 1712
BsnI GGCC 4 cut(s) 447, 1628, 1875, 2018
BsoBI CYCGRG 2 cut(s) 166, 223
Bsp119I TTCGAA 1 cut(s) 1107
Bsp1286I GDGCHC 6 cut(s) 112, 165, 1370, 1611, 2009, 2052
Bsp143I GATC 7 cut(s) 16, 183, 539, 570, 1012, 1504, 1658
BspANI GGCC 4 cut(s) 447, 1628, 1875, 2018
BspCNI CTCAG 5 cut(s) 248, 340, 838, 1134, 1295
BspFNI CGCG 1 cut(s) 122
BspHI TCATGA 2 cut(s) 288, 1578
BspLI GGNNCC 6 cut(s) 480, 866, 1170, 1725, 1935, 1950
BspOI GCTAGC 3 cut(s) 326, 1118, 1589
BspPI GGATC 4 cut(s) 24, 547, 1499, 1666
BspQI GCTCTTC 2 cut(s) 1104, 2040
BspT104I TTCGAA 1 cut(s) 1107
BspT107I GGYRCC 1 cut(s) 1723
BspTI CTTAAG 1 cut(s) 994
BsrBI CCGCTC 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 2144
BsrI ACTGG 5 cut(s) 301, 830, 936, 1067, 1749
BssECI CCNNGG 6 cut(s) 120, 167, 1084, 1508, 1870, 2013
BssMI GATC 7 cut(s) 16, 183, 539, 570, 1012, 1504, 1658
BssSI CACGAG 2 cut(s) 1157, 1830
BssT1I CCWWGG 2 cut(s) 1084, 1508
Bst2BI CACGAG 2 cut(s) 1157, 1830
Bst2UI CCWGG 1 cut(s) 314
Bst6I CTCTTC 6 cut(s) 254, 363, 468, 495, 1104, 2040
BstAFI CTTAAG 1 cut(s) 994
BstBI TTCGAA 1 cut(s) 1107
BstC8I GCNNGC 9 cut(s) 324, 577, 608, 923, 1058, 1116, 1236, 1475, 1587
BstDEI CTNAG 7 cut(s) 200, 235, 327, 846, 1142, 1239, 1282
BstDSI CCRYGG 3 cut(s) 120, 1870, 2013
BstEII GGTNACC 1 cut(s) 1798
BstENI CCTNNNNNAGG 2 cut(s) 199, 2103
BstF5I GGATG 4 cut(s) 1077, 1301, 1540, 1793
BstFNI CGCG 1 cut(s) 122
BstH2I RGCGCY 2 cut(s) 1289, 2141
BstHHI GCGC 2 cut(s) 1288, 2140
BstKTI GATC 7 cut(s) 19, 186, 542, 573, 1015, 1507, 1661
BstMAI GTCTC 1 cut(s) 768
BstMBI GATC 7 cut(s) 16, 183, 539, 570, 1012, 1504, 1658
BstMCI CGRYCG 2 cut(s) 1629, 1876
BstMWI GCNNNNNNNGC 9 cut(s) 29, 38, 116, 128, 1053, 1244, 1606, 1716, 2146
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 4 cut(s) 731, 925, 1060, 1772
BstPI GGTNACC 1 cut(s) 1798
BstSCI CCNGG 2 cut(s) 312, 1223
BstSFI CTRYAG 2 cut(s) 560, 898
BstSLI GKGCMC 1 cut(s) 2009
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 4 cut(s) 331, 402, 568, 1408
BstX2I RGATCY 1 cut(s) 539
BstXI CCANNNNNNTGG 1 cut(s) 1547
BstYI RGATCY 1 cut(s) 539
BstZI CGGCCG 2 cut(s) 1626, 1873
BsuRI GGCC 4 cut(s) 447, 1628, 1875, 2018
BtgI CCRYGG 3 cut(s) 120, 1870, 2013
BtgZI GCGATG 1 cut(s) 43
BtsCI GGATG 4 cut(s) 1077, 1301, 1540, 1793
BtsIMutI CAGTG 7 cut(s) 294, 579, 831, 953, 1060, 1742, 2009
Cac8I GCNNGC 9 cut(s) 324, 577, 608, 923, 1058, 1116, 1236, 1475, 1587
CciI TCATGA 2 cut(s) 288, 1578
CfoI GCGC 2 cut(s) 1288, 2140
Cfr13I GGNCC 1 cut(s) 865
Cfr42I CCGCGG 1 cut(s) 123
CseI GACGC 2 cut(s) 263, 1466
CspCI CAANNNNNGTGG 2 cut(s) 1630, 1665
DdeI CTNAG 7 cut(s) 200, 235, 327, 846, 1142, 1239, 1282
DpnI GATC 7 cut(s) 18, 185, 541, 572, 1014, 1506, 1660
DpnII GATC 7 cut(s) 16, 183, 539, 570, 1012, 1504, 1658
DrdI GACNNNNNNGTC 1 cut(s) 428
DriI GACNNNNNGTC 1 cut(s) 1832
DseDI GACNNNNNNGTC 1 cut(s) 428
EaeI YGGCCR 2 cut(s) 1626, 1873
EagI CGGCCG 2 cut(s) 1626, 1873
Eam1104I CTCTTC 6 cut(s) 254, 363, 468, 495, 1104, 2040
Eam1105I GACNNNNNGTC 1 cut(s) 1832
EarI CTCTTC 6 cut(s) 254, 363, 468, 495, 1104, 2040
EciI GGCGGA 1 cut(s) 118
Ecl136II GAGCTC 2 cut(s) 110, 1609
EclXI CGGCCG 2 cut(s) 1626, 1873
Eco130I CCWWGG 2 cut(s) 1084, 1508
Eco24I GRGCYC 3 cut(s) 112, 1611, 2052
Eco47I GGWCC 1 cut(s) 865
Eco47III AGCGCT 1 cut(s) 2139
Eco52I CGGCCG 2 cut(s) 1626, 1873
Eco53kI GAGCTC 2 cut(s) 110, 1609
Eco57I CTGAAG 2 cut(s) 1503, 2121
Eco88I CYCGRG 2 cut(s) 166, 223
Eco91I GGTNACC 1 cut(s) 1798
EcoICRI GAGCTC 2 cut(s) 110, 1609
EcoNI CCTNNNNNAGG 2 cut(s) 199, 2103
EcoO65I GGTNACC 1 cut(s) 1798
EcoRI GAATTC 1 cut(s) 881
EcoRII CCWGG 1 cut(s) 312
EcoT14I CCWWGG 2 cut(s) 1084, 1508
EcoT38I GRGCYC 3 cut(s) 112, 1611, 2052
ErhI CCWWGG 2 cut(s) 1084, 1508
FalI AAGNNNNNCTT 8 cut(s) 802, 834, 1002, 1034, 1324, 1356, 1355, 1387
FaqI GGGAC 3 cut(s) 226, 778, 878
FauI CCCGC 1 cut(s) 1243
FauNDI CATATG 1 cut(s) 1924
FbaI TGATCA 1 cut(s) 183
FblI GTMKAC 5 cut(s) 432, 465, 969, 1215, 1257
FokI GGATG 4 cut(s) 1064, 1288, 1527, 1800
FriOI GRGCYC 3 cut(s) 112, 1611, 2052
GlaI GCGC 2 cut(s) 1287, 2139
GsaI CCCAGC 1 cut(s) 765
GsuI CTGGAG 2 cut(s) 318, 1766
HaeII RGCGCY 2 cut(s) 1289, 2141
HaeIII GGCC 4 cut(s) 447, 1628, 1875, 2018
HapII CCGG 1 cut(s) 1224
HgaI GACGC 2 cut(s) 263, 1466
HhaI GCGC 2 cut(s) 1288, 2140
Hin6I GCGC 2 cut(s) 1286, 2138
HinP1I GCGC 2 cut(s) 1286, 2138
HincII GTYRAC 5 cut(s) 400, 433, 466, 970, 1258
HindII GTYRAC 5 cut(s) 400, 433, 466, 970, 1258
HindIII AAGCTT 1 cut(s) 1245
HinfI GANTC 8 cut(s) 142, 239, 260, 614, 848, 1409, 1492, 1833
HpaII CCGG 1 cut(s) 1224
HphI GGTGA 5 cut(s) 83, 178, 1243, 1465, 1859
Hpy166II GTNNAC 8 cut(s) 400, 433, 466, 831, 970, 1216, 1258, 2007
Hpy188I TCNGA 4 cut(s) 238, 330, 1024, 1414
Hpy8I GTNNAC 8 cut(s) 400, 433, 466, 831, 970, 1216, 1258, 2007
Hpy99I CGWCG 5 cut(s) 257, 470, 912, 971, 1259
HpyAV CCTTC 6 cut(s) 367, 593, 1312, 1350, 1778, 2099
HpyCH4IV ACGT 1 cut(s) 1394
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 38, 116, 128, 1053, 1244, 1606, 1716, 2146
HpyF3I CTNAG 7 cut(s) 200, 235, 327, 846, 1142, 1239, 1282
HpySE526I ACGT 1 cut(s) 1394
HspAI GCGC 2 cut(s) 1286, 2138
Ksp22I TGATCA 1 cut(s) 183
KspI CCGCGG 1 cut(s) 123
Kzo9I GATC 7 cut(s) 16, 183, 539, 570, 1012, 1504, 1658
LguI GCTCTTC 2 cut(s) 1104, 2040
LmnI GCTCC 7 cut(s) 299, 316, 478, 1138, 1168, 1942, 1948
Lsp1109I GCAGC 4 cut(s) 331, 402, 568, 1408
LweI GCATC 7 cut(s) 14, 93, 215, 643, 654, 1753, 2136
MaeII ACGT 1 cut(s) 1394
MaeIII GTNAC 4 cut(s) 812, 1068, 1798, 2021
MalI GATC 7 cut(s) 18, 185, 541, 572, 1014, 1506, 1660
MbiI CCGCTC 1 cut(s) 267
MboI GATC 7 cut(s) 16, 183, 539, 570, 1012, 1504, 1658
MfeI CAATTG 1 cut(s) 1647
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 6 cut(s) 112, 165, 1370, 1611, 2009, 2052
MluCI AATT 5 cut(s) 689, 791, 881, 1602, 1647
MlyI GAGTC 6 cut(s) 136, 248, 608, 857, 1403, 1842
MmeI TCCRAC 2 cut(s) 1362, 2023
MseI TTAA 4 cut(s) 452, 995, 1458, 1665
MslI CAYNNNNRTG 3 cut(s) 1040, 1773, 2012
MspA1I CMGCKG 2 cut(s) 122, 488
MspCI CTTAAG 1 cut(s) 994
MspI CCGG 1 cut(s) 1224
MspR9I CCNGG 2 cut(s) 314, 1225
MunI CAATTG 1 cut(s) 1647
Mva1269I GAATGC 2 cut(s) 1475, 1712
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 9 cut(s) 29, 38, 116, 128, 1053, 1244, 1606, 1716, 2146
NciI CCSGG 1 cut(s) 1225
NdeI CATATG 1 cut(s) 1924
NdeII GATC 7 cut(s) 16, 183, 539, 570, 1012, 1504, 1658
NheI GCTAGC 3 cut(s) 322, 1114, 1585
NlaIV GGNNCC 6 cut(s) 480, 866, 1170, 1725, 1935, 1950
NmuCI GTSAC 1 cut(s) 2021
NspI RCATGY 4 cut(s) 731, 925, 1060, 1772
NspV TTCGAA 1 cut(s) 1107
OliI CACNNNNGTG 1 cut(s) 2012
PaeI GCATGC 2 cut(s) 925, 1060
PagI TCATGA 2 cut(s) 288, 1578
PciI ACATGT 1 cut(s) 727
PciSI GCTCTTC 2 cut(s) 1104, 2040
PcsI WCGNNNNNNNCGW 1 cut(s) 1155
PctI GAATGC 2 cut(s) 1475, 1712
PfeI GAWTC 2 cut(s) 260, 1492
PflFI GACNNNGTC 1 cut(s) 145
PflMI CCANNNNNTGG 2 cut(s) 443, 1135
PfoI TCCNGGA 1 cut(s) 312
PleI GAGTC 6 cut(s) 136, 247, 608, 856, 1403, 1841
PpsI GAGTC 6 cut(s) 136, 247, 608, 856, 1403, 1841
PscI ACATGT 1 cut(s) 727
Psp124BI GAGCTC 2 cut(s) 112, 1611
Psp6I CCWGG 1 cut(s) 312
PspEI GGTNACC 1 cut(s) 1798
PspFI CCCAGC 1 cut(s) 761
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 6 cut(s) 480, 866, 1170, 1725, 1935, 1950
PspPI GGNCC 1 cut(s) 865
PsuI RGATCY 1 cut(s) 539
PsyI GACNNNGTC 1 cut(s) 145
RseI CAYNNNNRTG 3 cut(s) 1040, 1773, 2012
SacI GAGCTC 2 cut(s) 112, 1611
SacII CCGCGG 1 cut(s) 123
SalI GTCGAC 4 cut(s) 431, 464, 968, 1256
SapI GCTCTTC 2 cut(s) 1104, 2040
SaqAI TTAA 4 cut(s) 452, 995, 1458, 1665
Sau3AI GATC 7 cut(s) 16, 183, 539, 570, 1012, 1504, 1658
Sau96I GGNCC 1 cut(s) 865
ScaI AGTACT 1 cut(s) 1979
SchI GAGTC 6 cut(s) 136, 248, 608, 857, 1403, 1842
ScrFI CCNGG 2 cut(s) 314, 1225
SduI GDGCHC 6 cut(s) 112, 165, 1370, 1611, 2009, 2052
SfaNI GCATC 7 cut(s) 14, 93, 215, 643, 654, 1753, 2136
SfcI CTRYAG 2 cut(s) 560, 898
Sfr303I CCGCGG 1 cut(s) 123
SfuI TTCGAA 1 cut(s) 1107
SgrBI CCGCGG 1 cut(s) 123
SinI GGWCC 1 cut(s) 865
SmiMI CAYNNNNRTG 3 cut(s) 1040, 1773, 2012
SmlI CTYRAG 4 cut(s) 994, 1372, 1616, 1711
SmoI CTYRAG 4 cut(s) 994, 1372, 1616, 1711
SphI GCATGC 2 cut(s) 925, 1060
Sse9I AATT 5 cut(s) 689, 791, 881, 1602, 1647
SstI GAGCTC 2 cut(s) 112, 1611
StyD4I CCNGG 2 cut(s) 312, 1223
StyI CCWWGG 2 cut(s) 1084, 1508
TaiI ACGT 1 cut(s) 1397
TasI AATT 5 cut(s) 689, 791, 881, 1602, 1647
TatI WGTACW 3 cut(s) 272, 624, 1977
TauI GCSGC 7 cut(s) 65, 122, 125, 270, 392, 1631, 1878
TfiI GAWTC 2 cut(s) 260, 1492
Tru1I TTAA 4 cut(s) 452, 995, 1458, 1665
Tru9I TTAA 4 cut(s) 452, 995, 1458, 1665
TscAI CASTG 7 cut(s) 301, 586, 838, 953, 1067, 1749, 2009
TseFI GTSAC 1 cut(s) 2021
TseI GCWGC 4 cut(s) 319, 415, 556, 1421
Tsp45I GTSAC 1 cut(s) 2021
TspGWI ACGGA 2 cut(s) 373, 607
TspRI CASTG 7 cut(s) 301, 586, 838, 953, 1067, 1749, 2009
Tth111I GACNNNGTC 1 cut(s) 145
Van91I CCANNNNNTGG 2 cut(s) 443, 1135
Vha464I CTTAAG 1 cut(s) 994
VneI GTGCAC 1 cut(s) 2005
VpaK11BI GGWCC 1 cut(s) 865
XagI CCTNNNNNAGG 2 cut(s) 199, 2103
XapI RAATTY 2 cut(s) 689, 881
XceI RCATGY 4 cut(s) 731, 925, 1060, 1772
XmaJI CCTAGG 1 cut(s) 1508
XmiI GTMKAC 5 cut(s) 432, 465, 969, 1215, 1257
ZrmI AGTACT 1 cut(s) 1979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.