pycom03g19250

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000128
Physical Location & Seq
Forward (+)
19903549 .. 19906124
2576 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g19250.9

Sequence Viewer

Length: 1923 bp
ATGAGCATGGAGCATTCTCATTCACCACTCACAGCTGAGGTAGATAGGCAGAGATCAATGATTGCTCAAGGTACCTTTGACCATAGGAAAAATTATTTACACAAATATCTTAGAATATTTCTACTAAAATACCACCTAGGGCTGACCCACTTTATCTCCAAAATCCAAGAAGCCTTTCGCCATGTAGAAAAGCCAAACACTCATTCTCTCAAAGTTTCTGACTTAAGTATTGACCAAACCATCCGTGCACACTCTGATGTGACTGCAACGTTAGACAACTTGCTGGAAAAAAAGGTGATCGAGCTACCCGAATGCAAGCACCCCGAAGAAATGAATCGCATTAACAATTCTAGGTACTGCAAGTACCATCGTATTGTGAGCCATCCTGTTGGCAAATGCTTCGTCCTCAAGGAGTTCTTCATGAAGCTGGCACAACAAGGGATTGCGTTTGGATCCTTCAATCCTGTGCCTCTTCAAGTGACGCCTAACCACTCCCGTCCATGCTCAAGCTACATGACACCTTATGCACAACCATCGCTAAGGGCAAGCGACCAAAATGCACATACATTCGATAAAGAATGGTGGACGCTGGTGACCTACAAAAAGACAAGGAAGCCAATACCACAAGCCACACGGCCAAAGGTGGAACAAGGGAGAAAGCACCACCGCCGCAACAATAGGAAGCCCAAAAGAAATGTAAGAGCTACTAAGCCAACATACGCTGGGGAACCTATGGAGAAAGAGTCGCACATTCCTGTCTCCTTACACGAGTACTTCCCAAATGACTTCTTCCAACAATGCACTACTGCTACCTGCCACATGGTTGAAGTAGAGATAGAAGAGCCCTCAAAAGGAAAAGATATCACCATCGAGGAAGAGAAGACCCTCACGCCCAAAGAAAGCCTATCGGCACACTTTAGCATCGAGGAAGCACTACAATTACTTAAGGAGATACAAAAAGCATTAGTAGCGGTCTTGGCGAGTCCCGACAACTACAAAGTGCAAGAAAGCAAAGCCCATGACGCAATCAACTTCACTGACAAAGACTTGCTGCTAGGATCAATGCCTCACAACCGTCCTATGTTCGTCTCCGGGTACGTGAGAGAGAACAAAATCAACCGCATGCTTATGGATGGTGGGTCGGCCATAAACATTAGAAATTTTAGAATTTTGTTTCCGGGATTTATTATAATTTACGTCGGAAAATTTATCGATAATTTACGAGGACGTATTTTATCCTTATATATTATATCATATTTCTTGACATATTTGGATAAAGGACGATTCCACAAGCGTGTAGGTGAAAACTGTTCGACAACCAGAGTTATAACAAATGAAATAGAAACGAACGAAAGTAGAGGCAAAATAGAGGTGTGTTTTGGTAGAAATTTGGCTAGAGGTGTGTTTTGGCTGGAAGGTTCCATAGTTCTAGAAGAAGGGGAAGAAAGGAGGAAAACAGAAGGGGAAGAAGGGGGAGAGTTCTGGAAGAAAGGGGGGGGAGAAAAAGAAGAAAAGAAAAGAAAGAGAAAAAGAAGAATGAAGGGGACTTTAACCCAAATTGCTCTCTCTCGCTATTATCTGTCGCTCAAACTCACCGTATTTGACTTCCCCTGTTTGGTTTTCAAAGAAGATAGTAAAACAGTAGGAGCTACAATTCTCCAATTCACATCTCTTTCATCTGTGTTAATCAGTTTGAATTTTATTGGGCATCTTGGTTATACTTTATACCCACATCATAATTGTTTTAATTCCTTAATCCTTGTACCAAGCAAGACCAACTGTCATGAATTATTTCTTGCCTTATTTCTAGCATACCATATGATGGAATTATCTCTCTGGGAAATTCAATGCTTCACATTGTCAACGTTTATCTTCTCTCCTAAATTCTTCTTAGTATTAAATTTTGAATCTTGGTTCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

641

Amino Acids

74.19

Weight (kDa)

9.26

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1190, 1328
Acc36I ACCTGC 1 cut(s) 821
Acc65I GGTACC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 1822
AciI CCGC 4 cut(s) 667, 670, 971, 1120
AclI AACGTT 2 cut(s) 269, 1865
AclWI GGATC 3 cut(s) 447, 460, 1066
AcoI YGGCCR 2 cut(s) 635, 1143
AcsI RAATTY 8 cut(s) 1159, 1167, 1205, 1387, 1696, 1842, 1883, 1900
AcyI GRCGYC 1 cut(s) 482
AfaI GTAC 6 cut(s) 73, 356, 365, 773, 1097, 1764
AfiI CCNNNNNNNGG 3 cut(s) 851, 1615, 1822
AflII CTTAAG 2 cut(s) 223, 944
AgsI TTSAA 7 cut(s) 460, 476, 827, 1624, 1696, 1847, 1907
AhdI GACNNNNNGTC 1 cut(s) 1779
AjuI GAANNNNNNNTTGG 4 cut(s) 159, 187, 191, 219
AleI CACNNNNGTG 1 cut(s) 1293
AluBI AGCT 6 cut(s) 35, 304, 427, 510, 704, 1649
AluI AGCT 6 cut(s) 35, 304, 427, 510, 704, 1649
Alw21I GWGCWC 1 cut(s) 250
Alw26I GTCTC 2 cut(s) 763, 1093
Alw44I GTGCAC 1 cut(s) 246
AlwI GGATC 3 cut(s) 447, 460, 1066
AoxI GGCC 2 cut(s) 635, 1143
ApaLI GTGCAC 1 cut(s) 246
ApeKI GCWGC 1 cut(s) 1051
ApoI RAATTY 8 cut(s) 1159, 1167, 1205, 1387, 1696, 1842, 1883, 1900
ArsI GACNNNNNNTTYG 2 cut(s) 387, 419
Asp700I GAANNNNTTC 2 cut(s) 174, 1791
Asp718I GGTACC 1 cut(s) 71
AspA2I CCTAGG 1 cut(s) 136
AsuC2I CCSGG 2 cut(s) 1093, 1179
AsuHPI GGTGA 6 cut(s) 15, 307, 604, 856, 1313, 1585
AvrII CCTAGG 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 250
BamHI GGATCC 1 cut(s) 452
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 1 cut(s) 846
BauI CACGAG 1 cut(s) 767
BbsI GAAGAC 1 cut(s) 887
Bbv12I GWGCWC 1 cut(s) 250
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 1038
BccI CCATC 7 cut(s) 248, 375, 390, 541, 875, 1127, 1816
BceAI ACGGC 1 cut(s) 650
BcgI CGANNNNNNTGC 6 cut(s) 382, 416, 516, 539, 550, 573
BcnI CCSGG 2 cut(s) 1093, 1179
BcoDI GTCTC 2 cut(s) 763, 1093
BfaI CTAG 6 cut(s) 137, 351, 1055, 1395, 1430, 1808
BfrI CTTAAG 2 cut(s) 223, 944
BfuAI ACCTGC 1 cut(s) 821
BisI GCNGC 2 cut(s) 670, 1052
BlnI CCTAGG 1 cut(s) 136
BlsI GCNGC 2 cut(s) 671, 1053
BmcAI AGTACT 1 cut(s) 773
Bme1390I CCNGG 2 cut(s) 1093, 1179
BmeRI GACNNNNNGTC 1 cut(s) 1779
BmiI GGNNCC 4 cut(s) 73, 454, 729, 1420
BmrFI CCNGG 2 cut(s) 1093, 1179
BmsI GCATC 2 cut(s) 930, 1717
BpiI GAAGAC 1 cut(s) 887
Bpu10I CCTNAGC 2 cut(s) 36, 539
BpuEI CTTGAG 3 cut(s) 51, 392, 490
BpuMI CCSGG 2 cut(s) 1093, 1179
Bsa29I ATCGAT 1 cut(s) 1212
BsaAI YACGTR 1 cut(s) 1099
BsaHI GRCGYC 1 cut(s) 482
BsaJI CCNNGG 1 cut(s) 136
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 728, 758
Bsc4I CCNNNNNNNGG 3 cut(s) 851, 1615, 1822
BseCI ATCGAT 1 cut(s) 1212
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 3 cut(s) 240, 382, 1138
BseLI CCNNNNNNNGG 3 cut(s) 851, 1615, 1822
BseMII CTCAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 1 cut(s) 1038
BseYI CCCAGC 1 cut(s) 722
BshFI GGCC 2 cut(s) 637, 1145
BshNI GGYRCC 1 cut(s) 71
BshVI ATCGAT 1 cut(s) 1212
BsiHKAI GWGCWC 1 cut(s) 250
BsiSI CCGG 2 cut(s) 1092, 1178
BslFI GGGAC 2 cut(s) 969, 1558
BslI CCNNNNNNNGG 3 cut(s) 851, 1615, 1822
BsmAI GTCTC 2 cut(s) 763, 1093
BsmBI CGTCTC 1 cut(s) 1093
BsmFI GGGAC 2 cut(s) 969, 1558
BsmI GAATGC 2 cut(s) 13, 317
BsnI GGCC 2 cut(s) 637, 1145
Bsp1286I GDGCHC 2 cut(s) 250, 846
Bsp143I GATC 4 cut(s) 53, 297, 452, 1058
BspACI CCGC 4 cut(s) 667, 670, 971, 1120
BspANI GGCC 2 cut(s) 637, 1145
BspCNI CTCAG 1 cut(s) 28
BspDI ATCGAT 1 cut(s) 1212
BspHI TCATGA 2 cut(s) 420, 1783
BspLI GGNNCC 4 cut(s) 73, 454, 729, 1420
BspMI ACCTGC 1 cut(s) 821
BspPI GGATC 3 cut(s) 447, 460, 1066
BspQI GCTCTTC 1 cut(s) 834
BspT107I GGYRCC 1 cut(s) 71
BspTI CTTAAG 2 cut(s) 223, 944
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 4 cut(s) 53, 297, 452, 1058
BssNI GRCGYC 1 cut(s) 482
BssSI CACGAG 1 cut(s) 767
BssT1I CCWWGG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 767
Bst4CI ACNGT 5 cut(s) 1076, 1310, 1597, 1642, 1781
Bst6I CTCTTC 3 cut(s) 477, 834, 870
BstACI GRCGYC 1 cut(s) 482
BstAFI CTTAAG 2 cut(s) 223, 944
BstBAI YACGTR 1 cut(s) 1099
BstC8I GCNNGC 4 cut(s) 317, 429, 547, 1124
BstDEI CTNAG 5 cut(s) 36, 110, 539, 708, 1891
BstEII GGTNACC 1 cut(s) 592
BstF5I GGATG 3 cut(s) 240, 382, 1138
BstKTI GATC 4 cut(s) 56, 300, 455, 1061
BstMAI GTCTC 2 cut(s) 763, 1093
BstMBI GATC 4 cut(s) 53, 297, 452, 1058
BstMWI GCNNNNNNNGC 3 cut(s) 968, 977, 1022
BstNSI RCATGY 1 cut(s) 1126
BstPI GGTNACC 1 cut(s) 592
BstSCI CCNGG 2 cut(s) 1091, 1177
BstSLI GKGCMC 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 1038
BstV2I GAAGAC 1 cut(s) 887
BstX2I RGATCY 1 cut(s) 452
BstXI CCANNNNNNTGG 1 cut(s) 389
BstYI RGATCY 1 cut(s) 452
Bsu15I ATCGAT 1 cut(s) 1212
BsuRI GGCC 2 cut(s) 637, 1145
BsuTUI ATCGAT 1 cut(s) 1212
BtgZI GCGATG 1 cut(s) 519
BtsCI GGATG 3 cut(s) 240, 382, 1138
BtsIMutI CAGTG 1 cut(s) 1035
BveI ACCTGC 1 cut(s) 821
Cac8I GCNNGC 4 cut(s) 317, 429, 547, 1124
CciI TCATGA 2 cut(s) 420, 1783
ClaI ATCGAT 1 cut(s) 1212
CseI GACGC 3 cut(s) 490, 595, 1031
Csp6I GTAC 6 cut(s) 72, 355, 364, 772, 1096, 1763
CviAII CATG 9 cut(s) 7, 182, 421, 501, 514, 820, 1019, 1123, 1784
CviQI GTAC 6 cut(s) 72, 355, 364, 772, 1096, 1763
DdeI CTNAG 5 cut(s) 36, 110, 539, 708, 1891
DpnI GATC 4 cut(s) 55, 299, 454, 1060
DpnII GATC 4 cut(s) 53, 297, 452, 1058
DriI GACNNNNNGTC 1 cut(s) 1779
EaeI YGGCCR 2 cut(s) 635, 1143
Eam1104I CTCTTC 3 cut(s) 477, 834, 870
Eam1105I GACNNNNNGTC 1 cut(s) 1779
EarI CTCTTC 3 cut(s) 477, 834, 870
Eco130I CCWWGG 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 846
Eco32I GATATC 1 cut(s) 862
Eco91I GGTNACC 1 cut(s) 592
EcoO65I GGTNACC 1 cut(s) 592
EcoRV GATATC 1 cut(s) 862
EcoT14I CCWWGG 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 846
ErhI CCWWGG 1 cut(s) 136
Esp3I CGTCTC 1 cut(s) 1093
FaeI CATG 9 cut(s) 10, 185, 424, 504, 517, 823, 1022, 1126, 1787
FalI AAGNNNNNCTT 2 cut(s) 401, 433
FaqI GGGAC 2 cut(s) 969, 1558
FatI CATG 9 cut(s) 6, 181, 420, 500, 513, 819, 1018, 1122, 1783
FauNDI CATATG 1 cut(s) 1818
Fnu4HI GCNGC 2 cut(s) 670, 1052
FokI GGATG 3 cut(s) 227, 369, 1145
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 2 cut(s) 670, 1052
FspBI CTAG 6 cut(s) 137, 351, 1055, 1395, 1430, 1808
GluI GCNGC 2 cut(s) 670, 1052
GsaI CCCAGC 1 cut(s) 726
HaeIII GGCC 2 cut(s) 637, 1145
HapII CCGG 2 cut(s) 1092, 1178
HgaI GACGC 3 cut(s) 490, 595, 1031
Hin1I GRCGYC 1 cut(s) 482
Hin1II CATG 9 cut(s) 10, 185, 424, 504, 517, 823, 1022, 1126, 1787
HincII GTYRAC 1 cut(s) 1863
HindII GTYRAC 1 cut(s) 1863
HinfI GANTC 5 cut(s) 334, 743, 982, 1284, 1907
HpaII CCGG 2 cut(s) 1092, 1178
HphI GGTGA 6 cut(s) 15, 307, 604, 856, 1313, 1585
Hpy166II GTNNAC 3 cut(s) 248, 585, 1863
Hpy188I TCNGA 3 cut(s) 220, 256, 1202
Hpy188III TCNNGA 6 cut(s) 421, 986, 1261, 1430, 1483, 1784
Hpy8I GTNNAC 3 cut(s) 248, 585, 1863
Hpy99I CGWCG 1 cut(s) 1202
HpyAV CCTTC 6 cut(s) 466, 1409, 1430, 1454, 1463, 1534
HpyCH4III ACNGT 5 cut(s) 1076, 1310, 1597, 1642, 1781
HpyCH4IV ACGT 5 cut(s) 269, 1098, 1197, 1228, 1865
HpyCH4V TGCA 8 cut(s) 248, 266, 315, 360, 527, 560, 801, 1003
HpyF10VI GCNNNNNNNGC 3 cut(s) 968, 977, 1022
HpyF3I CTNAG 5 cut(s) 36, 110, 539, 708, 1891
HpySE526I ACGT 5 cut(s) 269, 1098, 1197, 1228, 1865
Hsp92I GRCGYC 1 cut(s) 482
Hsp92II CATG 9 cut(s) 10, 185, 424, 504, 517, 823, 1022, 1126, 1787
KpnI GGTACC 1 cut(s) 75
Kzo9I GATC 4 cut(s) 53, 297, 452, 1058
LguI GCTCTTC 1 cut(s) 834
LmnI GCTCC 2 cut(s) 10, 1646
Lsp1109I GCAGC 1 cut(s) 1038
LweI GCATC 2 cut(s) 930, 1717
MaeI CTAG 6 cut(s) 137, 351, 1055, 1395, 1430, 1808
MaeII ACGT 5 cut(s) 269, 1098, 1197, 1228, 1865
MaeIII GTNAC 3 cut(s) 259, 478, 592
MalI GATC 4 cut(s) 55, 299, 454, 1060
MboI GATC 4 cut(s) 53, 297, 452, 1058
MflI RGATCY 1 cut(s) 452
MhlI GDGCHC 2 cut(s) 250, 846
MlyI GAGTC 2 cut(s) 752, 991
MmeI TCCRAC 2 cut(s) 817, 1180
MroXI GAANNNNTTC 2 cut(s) 174, 1791
MseI TTAA 9 cut(s) 224, 342, 945, 1550, 1685, 1746, 1754, 1898, 1921
MslI CAYNNNNRTG 3 cut(s) 255, 1127, 1293
MspA1I CMGCKG 1 cut(s) 35
MspCI CTTAAG 2 cut(s) 223, 944
MspI CCGG 2 cut(s) 1092, 1178
MspR9I CCNGG 2 cut(s) 1093, 1179
Mva1269I GAATGC 2 cut(s) 13, 317
MwoI GCNNNNNNNGC 3 cut(s) 968, 977, 1022
NciI CCSGG 2 cut(s) 1093, 1179
NdeI CATATG 1 cut(s) 1818
NdeII GATC 4 cut(s) 53, 297, 452, 1058
NlaIII CATG 9 cut(s) 10, 185, 424, 504, 517, 823, 1022, 1126, 1787
NlaIV GGNNCC 4 cut(s) 73, 454, 729, 1420
NmuCI GTSAC 3 cut(s) 259, 478, 592
NspI RCATGY 1 cut(s) 1126
OliI CACNNNNGTG 1 cut(s) 1293
PaeI GCATGC 1 cut(s) 1126
PagI TCATGA 2 cut(s) 420, 1783
PciSI GCTCTTC 1 cut(s) 834
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PctI GAATGC 2 cut(s) 13, 317
PdmI GAANNNNTTC 2 cut(s) 174, 1791
PfeI GAWTC 3 cut(s) 334, 1284, 1907
PflMI CCANNNNNTGG 1 cut(s) 1822
PfoI TCCNGGA 1 cut(s) 1177
PkrI GCNGC 2 cut(s) 671, 1053
PleI GAGTC 2 cut(s) 751, 990
PpsI GAGTC 2 cut(s) 751, 990
Ppu21I YACGTR 1 cut(s) 1099
PsiI TTATAA 2 cut(s) 1190, 1328
Psp1406I AACGTT 2 cut(s) 269, 1865
PspEI GGTNACC 1 cut(s) 592
PspFI CCCAGC 1 cut(s) 722
PspN4I GGNNCC 4 cut(s) 73, 454, 729, 1420
PsuI RGATCY 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 6 cut(s) 73, 356, 365, 773, 1097, 1764
RsaNI GTAC 6 cut(s) 72, 355, 364, 772, 1096, 1763
RseI CAYNNNNRTG 3 cut(s) 255, 1127, 1293
SapI GCTCTTC 1 cut(s) 834
SaqAI TTAA 9 cut(s) 224, 342, 945, 1550, 1685, 1746, 1754, 1898, 1921
SatI GCNGC 2 cut(s) 670, 1052
Sau3AI GATC 4 cut(s) 53, 297, 452, 1058
ScaI AGTACT 1 cut(s) 773
SchI GAGTC 2 cut(s) 752, 991
ScrFI CCNGG 2 cut(s) 1093, 1179
SduI GDGCHC 2 cut(s) 250, 846
SfaNI GCATC 2 cut(s) 930, 1717
SmiMI CAYNNNNRTG 3 cut(s) 255, 1127, 1293
SmlI CTYRAG 5 cut(s) 66, 223, 407, 505, 944
SmoI CTYRAG 5 cut(s) 66, 223, 407, 505, 944
SphI GCATGC 1 cut(s) 1126
SsiI CCGC 4 cut(s) 667, 670, 971, 1120
SspI AATATT 1 cut(s) 117
SspMI CTAG 6 cut(s) 137, 351, 1055, 1395, 1430, 1808
StyD4I CCNGG 2 cut(s) 1091, 1177
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 5 cut(s) 1076, 1310, 1597, 1642, 1781
TaiI ACGT 5 cut(s) 272, 1101, 1200, 1231, 1868
TaqI TCGA 6 cut(s) 300, 570, 870, 924, 1212, 1313
TatI WGTACW 1 cut(s) 771
TauI GCSGC 1 cut(s) 672
TfiI GAWTC 3 cut(s) 334, 1284, 1907
Tru1I TTAA 9 cut(s) 224, 342, 945, 1550, 1685, 1746, 1754, 1898, 1921
Tru9I TTAA 9 cut(s) 224, 342, 945, 1550, 1685, 1746, 1754, 1898, 1921
TscAI CASTG 1 cut(s) 1042
TseFI GTSAC 3 cut(s) 259, 478, 592
TseI GCWGC 1 cut(s) 1051
Tsp45I GTSAC 3 cut(s) 259, 478, 592
TspDTI ATGAA 8 cut(s) 347, 409, 437, 1350, 1553, 1665, 1800, 1906
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 1 cut(s) 1042
Van91I CCANNNNNTGG 1 cut(s) 1822
Vha464I CTTAAG 2 cut(s) 223, 944
VneI GTGCAC 1 cut(s) 246
XapI RAATTY 8 cut(s) 1159, 1167, 1205, 1387, 1696, 1842, 1883, 1900
XbaI TCTAGA 1 cut(s) 1429
XceI RCATGY 1 cut(s) 1126
XmaJI CCTAGG 1 cut(s) 136
XmnI GAANNNNTTC 2 cut(s) 174, 1791
XspI CTAG 6 cut(s) 137, 351, 1055, 1395, 1430, 1808
ZrmI AGTACT 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.