pycom520g01020

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0000128
Physical Location & Seq
Reverse (-)
773275 .. 774323
1049 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g01020.2

Sequence Viewer

Length: 675 bp
ATGAATGAAGCAATCGCAAGGCTCACACGGACTGTGGAAGAGAAGGACTTGCAGATTGCCGCACTTGTCAACCAACTAGATGCGCTGCCCGATGTGAAAGTCGACCCAAATATTGGCCTATTAAAGAAAGAAGACGACGAAGAAGAGGAGCCACCAGTGGAGAAAGTTGAAGAGAAGCCGAAGCTAGACCAAGCAACGGCATTCATGGGATCTCTATCTATCCAGCAGCTACAGGAGATGATCGCAAGCACTGTCAAGACACAGTACGAAGGAAGCTCGCATGACTCCGTACTGTACTCAAAGCCTTACTCCAAGAAGATTGATGCTTTGAAGATGCCAAGGGGTTATCAGCCCCCAAAATTCATGCAGTTCGATGGAAAAGGCAACCCGAAGCAACATGTCGCCCATTTCATTGAAACTTGCAACAATGCTGGGACAGAGGGAGACTACCTCGTCAAACAGTTTGTGCGTTCGCTGAAAAGTAACGCTTTTGACTGGTACACTGACCTCGAACCTGAGTCTATCAACAGTTGGGACCAGCTAGAAAGGGAATTCCTCAACCGTTTCTACAACCTCCTTAGCGCCCATCAAGACCTCATCAGCGCCCATCAAGACCTCATCAGCACCCATCAAGATCTCCTCCAAGAAGAAAGCGAAAGAGATAAAGACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

225

Amino Acids

25.69

Weight (kDa)

4.82

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 452
AccB7I CCANNNNNTGG 1 cut(s) 113
AccI GTMKAC 1 cut(s) 102
AciI CCGC 1 cut(s) 60
AclWI GGATC 1 cut(s) 217
AcsI RAATTY 2 cut(s) 359, 551
AfaI GTAC 4 cut(s) 266, 291, 296, 500
AfiI CCNNNNNNNGG 2 cut(s) 113, 196
AflIII ACRYGT 1 cut(s) 397
AgsI TTSAA 3 cut(s) 170, 331, 416
AluBI AGCT 4 cut(s) 184, 229, 276, 541
AluI AGCT 4 cut(s) 184, 229, 276, 541
Alw26I GTCTC 1 cut(s) 438
AlwI GGATC 1 cut(s) 217
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 85, 226
ApoI RAATTY 2 cut(s) 359, 551
AspLEI GCGC 3 cut(s) 85, 584, 605
AspS9I GGNCC 1 cut(s) 535
AvaII GGWCC 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 72, 238
BccI CCATC 4 cut(s) 368, 594, 615, 636
BceAI ACGGC 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 3 cut(s) 77, 185, 542
BfmI CTRYAG 1 cut(s) 230
BfoI RGCGCY 2 cut(s) 585, 606
BglII AGATCT 1 cut(s) 634
BisI GCNGC 3 cut(s) 60, 86, 227
BlsI GCNGC 3 cut(s) 61, 87, 228
Bme18I GGWCC 1 cut(s) 535
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 2 cut(s) 150, 536
BmsI GCATC 3 cut(s) 70, 313, 324
BpiI GAAGAC 1 cut(s) 138
BplI GAGNNNNNCTC 2 cut(s) 435, 467
Bpu10I CCTNAGC 1 cut(s) 578
BsaBI GATNNNNATC 1 cut(s) 214
BsaJI CCNNGG 1 cut(s) 338
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 196
Bse1I ACTGG 2 cut(s) 155, 500
Bse8I GATNNNNATC 1 cut(s) 214
BseDI CCNNGG 1 cut(s) 338
BseJI GATNNNNATC 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 113, 196
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 2 cut(s) 155, 500
BseRI GAGGAG 2 cut(s) 161, 629
BseXI GCAGC 2 cut(s) 72, 238
BseYI CCCAGC 1 cut(s) 431
BshFI GGCC 1 cut(s) 117
BslFI GGGAC 2 cut(s) 448, 548
BslI CCNNNNNNNGG 2 cut(s) 113, 196
BsmAI GTCTC 1 cut(s) 438
BsmFI GGGAC 2 cut(s) 448, 548
BsmI GAATGC 1 cut(s) 200
BsnI GGCC 1 cut(s) 117
Bsp143I GATC 3 cut(s) 209, 240, 634
BspACI CCGC 1 cut(s) 60
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 508
BspLI GGNNCC 2 cut(s) 150, 536
BspPI GGATC 1 cut(s) 217
BsrI ACTGG 2 cut(s) 155, 500
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 3 cut(s) 209, 240, 634
BssT1I CCWWGG 1 cut(s) 338
Bst4CI ACNGT 7 cut(s) 34, 253, 264, 294, 462, 530, 563
Bst6I CTCTTC 3 cut(s) 33, 138, 165
BstC8I GCNNGC 2 cut(s) 247, 278
BstDEI CTNAG 2 cut(s) 516, 578
BstH2I RGCGCY 2 cut(s) 585, 606
BstHHI GCGC 3 cut(s) 85, 584, 605
BstKTI GATC 3 cut(s) 212, 243, 637
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 3 cut(s) 209, 240, 634
BstNSI RCATGY 1 cut(s) 401
BstSFI CTRYAG 1 cut(s) 230
BstV1I GCAGC 2 cut(s) 72, 238
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 2 cut(s) 209, 634
BstYI RGATCY 2 cut(s) 209, 634
BsuRI GGCC 1 cut(s) 117
BtsIMutI CAGTG 3 cut(s) 162, 249, 501
Cac8I GCNNGC 2 cut(s) 247, 278
CfoI GCGC 3 cut(s) 85, 584, 605
Cfr13I GGNCC 1 cut(s) 535
Csp6I GTAC 4 cut(s) 265, 290, 295, 499
CviAII CATG 4 cut(s) 205, 281, 364, 398
CviQI GTAC 4 cut(s) 265, 290, 295, 499
DdeI CTNAG 2 cut(s) 516, 578
DpnI GATC 3 cut(s) 211, 242, 636
DpnII GATC 3 cut(s) 209, 240, 634
DrdI GACNNNNNNGTC 1 cut(s) 452
DseDI GACNNNNNNGTC 1 cut(s) 452
Eam1104I CTCTTC 3 cut(s) 33, 138, 165
EarI CTCTTC 3 cut(s) 33, 138, 165
Eco130I CCWWGG 1 cut(s) 338
Eco47I GGWCC 1 cut(s) 535
EcoRI GAATTC 1 cut(s) 551
EcoT14I CCWWGG 1 cut(s) 338
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 4 cut(s) 208, 284, 367, 401
FaiI YATR 4 cut(s) 206, 282, 365, 399
FalI AAGNNNNNCTT 2 cut(s) 472, 504
FaqI GGGAC 2 cut(s) 448, 548
FatI CATG 4 cut(s) 204, 280, 363, 397
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 3 cut(s) 60, 86, 227
Fsp4HI GCNGC 3 cut(s) 60, 86, 227
FspBI CTAG 3 cut(s) 77, 185, 542
GlaI GCGC 3 cut(s) 84, 583, 604
GluI GCNGC 3 cut(s) 60, 86, 227
GsaI CCCAGC 1 cut(s) 435
HaeII RGCGCY 2 cut(s) 585, 606
HaeIII GGCC 1 cut(s) 117
HhaI GCGC 3 cut(s) 85, 584, 605
Hin1II CATG 4 cut(s) 208, 284, 367, 401
Hin6I GCGC 3 cut(s) 83, 582, 603
HinP1I GCGC 3 cut(s) 83, 582, 603
HincII GTYRAC 2 cut(s) 70, 103
HindII GTYRAC 2 cut(s) 70, 103
HinfI GANTC 2 cut(s) 284, 518
Hpy166II GTNNAC 3 cut(s) 70, 103, 501
Hpy188III TCNNGA 4 cut(s) 256, 590, 611, 632
Hpy8I GTNNAC 3 cut(s) 70, 103, 501
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 37, 263
HpyCH4III ACNGT 7 cut(s) 34, 253, 264, 294, 462, 530, 563
HpyCH4V TGCA 3 cut(s) 52, 367, 423
HpyF3I CTNAG 2 cut(s) 516, 578
Hsp92II CATG 4 cut(s) 208, 284, 367, 401
HspAI GCGC 3 cut(s) 83, 582, 603
Kzo9I GATC 3 cut(s) 209, 240, 634
LmnI GCTCC 1 cut(s) 148
LpnPI CCDG 7 cut(s) 168, 218, 236, 417, 481, 528, 551
Lsp1109I GCAGC 2 cut(s) 72, 238
LweI GCATC 3 cut(s) 70, 313, 324
MaeI CTAG 3 cut(s) 77, 185, 542
MaeIII GTNAC 1 cut(s) 482
MalI GATC 3 cut(s) 211, 242, 636
MboI GATC 3 cut(s) 209, 240, 634
MboII GAAGA 8 cut(s) 50, 143, 152, 155, 182, 328, 343, 659
MflI RGATCY 2 cut(s) 209, 634
MluCI AATT 2 cut(s) 359, 551
MlyI GAGTC 2 cut(s) 278, 527
MnlI CCTC 9 cut(s) 139, 433, 461, 518, 566, 584, 605, 626, 650
MseI TTAA 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 200
NdeII GATC 3 cut(s) 209, 240, 634
NlaIII CATG 4 cut(s) 208, 284, 367, 401
NlaIV GGNNCC 2 cut(s) 150, 536
NspI RCATGY 1 cut(s) 401
PciI ACATGT 1 cut(s) 397
PctI GAATGC 1 cut(s) 200
PflMI CCANNNNNTGG 1 cut(s) 113
PkrI GCNGC 3 cut(s) 61, 87, 228
PleI GAGTC 2 cut(s) 278, 526
PpsI GAGTC 2 cut(s) 278, 526
PscI ACATGT 1 cut(s) 397
PspFI CCCAGC 1 cut(s) 431
PspN4I GGNNCC 2 cut(s) 150, 536
PspPI GGNCC 1 cut(s) 535
PsuI RGATCY 2 cut(s) 209, 634
RsaI GTAC 4 cut(s) 266, 291, 296, 500
RsaNI GTAC 4 cut(s) 265, 290, 295, 499
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 1 cut(s) 122
SatI GCNGC 3 cut(s) 60, 86, 227
Sau3AI GATC 3 cut(s) 209, 240, 634
Sau96I GGNCC 1 cut(s) 535
SchI GAGTC 2 cut(s) 278, 527
SfaNI GCATC 3 cut(s) 70, 313, 324
SfcI CTRYAG 1 cut(s) 230
SinI GGWCC 1 cut(s) 535
Sse9I AATT 2 cut(s) 359, 551
SsiI CCGC 1 cut(s) 60
SspI AATATT 1 cut(s) 112
SspMI CTAG 3 cut(s) 77, 185, 542
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 7 cut(s) 34, 253, 264, 294, 462, 530, 563
TaqI TCGA 3 cut(s) 102, 372, 510
TasI AATT 2 cut(s) 359, 551
TatI WGTACW 1 cut(s) 294
TauI GCSGC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 122
Tru9I TTAA 1 cut(s) 122
TscAI CASTG 3 cut(s) 162, 256, 508
TseI GCWGC 2 cut(s) 85, 226
TspDTI ATGAA 5 cut(s) 17, 21, 193, 352, 400
TspGWI ACGGA 2 cut(s) 43, 277
TspRI CASTG 3 cut(s) 162, 256, 508
Van91I CCANNNNNTGG 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 535
XapI RAATTY 2 cut(s) 359, 551
XceI RCATGY 1 cut(s) 401
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 3 cut(s) 77, 185, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.