pycom17g27300

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000128
Physical Location & Seq
Forward (+)
25556260 .. 25557609
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g27300.1

Sequence Viewer

Length: 1350 bp
ATGGCATCAAGAAAGAATCAAGCTGTTCCTGCAATCAAAGCAAAGAACAAGAACATCATCGCCGCAAGTGGCGATATTTCGGGCATCATAACCCGAAGTAAGGCAAGAGCTCTTTTTGCCGCGGCTTCCGCCCCTGCATTAACTCTGTCAAAGGAACAAGAGCACCTGAGGTACGAGCCTGTGATCACCCTAGCCTCGCTAAGGGCATCAAGGGGGGAAAGCCCGAGGAAATACTCAGAGTCCCTACTCTCCGACGCTGATTCGAGCGGCAGTACAACCATGCAAGTCATGACCACTGGAGCAACTTCAATCGAGGAGCAGCTAGCTCAGATGAATGAAGCAATCGCAAAGCTCACACGGACTGTGGAAGAGAAGGACTTGCAGATTGCCGCACTTGTCAACCAACTAGATGCGCTGCCTGACGTGAAAGTCGACCCAAATGTTGGCCTATTAAAGAAAGAAGTCGACGAAAAAGAGGAGCCACCAGCGGAGAAAGTTGAAGAGAAGCCGAAGCTAGACCAAGCAACGGCATTCATGGGATCTCTCTCTATCCAGCAGCTACAGGAGATGATCGCAAGCACTATCAAGACACAGTACGAAAGAAGCTCGCATGACTCCGTACTGTACTCAAAGCCTTACTCCAAGAAGATTGATGCTTTGAAGATGCCAAGGGGTTATCAGCCCCCAAAATTCATGCAGTTCAATGGAAAAGGCAACCCGAAGCAACATGTCGCCCATTTCATTGAAACTTGCAACAATGCTGGGACAGAGGGAGACTACCTCGTCAAACAGTTCGTGCGTTCGCTGAAAAGTAACGCTTTTGACTGGTACACTGACCTCGAACCTGAGTCTATCAACAGTTGGGACCAGCTAGAAAGGGAATTCCTCAACCGTTTCTACAGCACTCGTCGTACTGTAAGCATGCTAGAGCTGACCAGTACGAAACAGTGGAGAGATGAACCTGTCGTCGACTACATAAATAGATGGCGCTCCTTAAGTCTGGATTGCAAAGATCGGCTTTCTGAAACCTCTGCCATTGAGATGTGCGTTCAAGGCATGCACTGGGGGTTACATTACATCCTCCAAGGCATAAAACCAAGAACATTCGAAGAGCTAGCAACTTGTTCCCACGATATGGAGCTGAGTATCGCTAATCACGGGAAAAATGAGCTGATTACTAACTTCAATAAGGATAAAGTATTCGCCCCAAATGTAGACAAGACCGGGAAGAAGCCCGCTAAGGAAGCTTTCACCGTCAACACTACCCCCATCAAGACGTCCTCAGCGCCCATCAAGACCTCCTCAGCGCCCATCAAGATCGATCCTCCAAGAAGAAAGCGAAGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

450

Amino Acids

50.43

Weight (kDa)

9.03

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 265 - 354 8.7e-14 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 428, 782
AatII GACGTC 1 cut(s) 1280
AccB7I CCANNNNNTGG 2 cut(s) 443, 1135
AccBSI CCGCTC 1 cut(s) 267
AccI GTMKAC 4 cut(s) 432, 465, 969, 1215
AccII CGCG 1 cut(s) 122
AciI CCGC 8 cut(s) 63, 120, 122, 129, 267, 390, 488, 1236
AclWI GGATC 2 cut(s) 547, 1316
AcsI RAATTY 2 cut(s) 689, 881
AcyI GRCGYC 1 cut(s) 1277
AfaI GTAC 8 cut(s) 173, 274, 596, 621, 626, 830, 913, 940
AfiI CCNNNNNNNGG 5 cut(s) 100, 201, 443, 526, 1135
AflII CTTAAG 1 cut(s) 994
AflIII ACRYGT 1 cut(s) 727
AgsI TTSAA 7 cut(s) 309, 500, 661, 703, 746, 1052, 1186
AjiI CACGTC 1 cut(s) 424
Alw21I GWGCWC 2 cut(s) 112, 165
Alw26I GTCTC 1 cut(s) 768
AlwI GGATC 2 cut(s) 547, 1316
Ama87I CYCGRG 1 cut(s) 223
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 3 cut(s) 319, 415, 556
ApoI RAATTY 2 cut(s) 689, 881
AspLEI GCGC 4 cut(s) 415, 990, 1288, 1309
AspS9I GGNCC 1 cut(s) 865
AsuC2I CCSGG 1 cut(s) 1225
AsuHPI GGTGA 2 cut(s) 178, 1243
AsuII TTCGAA 1 cut(s) 1107
AsuNHI GCTAGC 2 cut(s) 322, 1114
AvaI CYCGRG 1 cut(s) 223
AvaII GGWCC 1 cut(s) 865
AxyI CCTNAGG 1 cut(s) 167
BanII GRGCYC 1 cut(s) 112
Bbv12I GWGCWC 2 cut(s) 112, 165
BbvCI CCTCAGC 2 cut(s) 1282, 1303
BbvI GCAGC 3 cut(s) 331, 402, 568
BccI CCATC 4 cut(s) 978, 1277, 1298, 1319
BceAI ACGGC 1 cut(s) 543
BclI TGATCA 1 cut(s) 183
BcnI CCSGG 1 cut(s) 1225
BcoDI GTCTC 1 cut(s) 768
BfaI CTAG 7 cut(s) 191, 323, 407, 515, 872, 926, 1115
BfmI CTRYAG 2 cut(s) 560, 898
BfoI RGCGCY 3 cut(s) 991, 1289, 1310
BfrI CTTAAG 1 cut(s) 994
BisI GCNGC 8 cut(s) 63, 120, 123, 268, 320, 390, 416, 557
BlsI GCNGC 8 cut(s) 64, 121, 124, 269, 321, 391, 417, 558
Bme1390I CCNGG 1 cut(s) 1225
Bme18I GGWCC 1 cut(s) 865
BmeT110I CYCGRG 1 cut(s) 223
BmgBI CACGTC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 865
BmiI GGNNCC 2 cut(s) 480, 866
BmrFI CCNGG 1 cut(s) 1225
BmrI ACTGGG 1 cut(s) 1072
BmsI GCATC 6 cut(s) 14, 93, 215, 400, 643, 654
BmtI GCTAGC 2 cut(s) 326, 1118
BmuI ACTGGG 1 cut(s) 1072
BplI GAGNNNNNCTC 2 cut(s) 765, 797
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 4 cut(s) 200, 1239, 1282, 1303
Bpu14I TTCGAA 1 cut(s) 1107
BpuMI CCSGG 1 cut(s) 1225
Bsa29I ATCGAT 1 cut(s) 1320
BsaHI GRCGYC 1 cut(s) 1277
BsaJI CCNNGG 4 cut(s) 120, 224, 668, 1084
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 201, 443, 526, 1135
Bse1I ACTGG 4 cut(s) 301, 830, 936, 1067
Bse21I CCTNAGG 1 cut(s) 167
BseCI ATCGAT 1 cut(s) 1320
BseDI CCNNGG 4 cut(s) 120, 224, 668, 1084
BseGI GGATG 1 cut(s) 1077
BseLI CCNNNNNNNGG 5 cut(s) 100, 201, 443, 526, 1135
BseMII CTCAG 7 cut(s) 158, 249, 341, 837, 1133, 1296, 1317
BseNI ACTGG 4 cut(s) 301, 830, 936, 1067
BseRI GAGGAG 3 cut(s) 329, 491, 1291
BseXI GCAGC 3 cut(s) 331, 402, 568
BseYI CCCAGC 1 cut(s) 761
Bsh1236I CGCG 1 cut(s) 122
BshFI GGCC 1 cut(s) 447
BshVI ATCGAT 1 cut(s) 1320
BsiHKAI GWGCWC 2 cut(s) 112, 165
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 1224
BslFI GGGAC 3 cut(s) 226, 778, 878
BslI CCNNNNNNNGG 5 cut(s) 100, 201, 443, 526, 1135
BsmAI GTCTC 1 cut(s) 768
BsmFI GGGAC 3 cut(s) 226, 778, 878
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 1 cut(s) 447
BsoBI CYCGRG 1 cut(s) 223
Bsp119I TTCGAA 1 cut(s) 1107
Bsp1286I GDGCHC 2 cut(s) 112, 165
Bsp143I GATC 6 cut(s) 183, 539, 570, 1012, 1317, 1321
BspACI CCGC 8 cut(s) 63, 120, 122, 129, 267, 390, 488, 1236
BspANI GGCC 1 cut(s) 447
BspCNI CTCAG 7 cut(s) 159, 248, 340, 838, 1134, 1295, 1316
BspDI ATCGAT 1 cut(s) 1320
BspFNI CGCG 1 cut(s) 122
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 480, 866
BspOI GCTAGC 2 cut(s) 326, 1118
BspPI GGATC 2 cut(s) 547, 1316
BspQI GCTCTTC 1 cut(s) 1104
BspT104I TTCGAA 1 cut(s) 1107
BspTI CTTAAG 1 cut(s) 994
BsrBI CCGCTC 1 cut(s) 267
BsrI ACTGG 4 cut(s) 301, 830, 936, 1067
BssECI CCNNGG 4 cut(s) 120, 224, 668, 1084
BssMI GATC 6 cut(s) 183, 539, 570, 1012, 1317, 1321
BssNI GRCGYC 1 cut(s) 1277
BssT1I CCWWGG 2 cut(s) 668, 1084
Bst4CI ACNGT 9 cut(s) 364, 594, 624, 792, 860, 893, 916, 948, 1255
Bst6I CTCTTC 3 cut(s) 363, 495, 1104
BstACI GRCGYC 1 cut(s) 1277
BstAFI CTTAAG 1 cut(s) 994
BstBI TTCGAA 1 cut(s) 1107
BstC8I GCNNGC 7 cut(s) 324, 577, 608, 923, 1058, 1116, 1236
BstDEI CTNAG 9 cut(s) 167, 200, 235, 327, 846, 1142, 1239, 1282, 1303
BstDSI CCRYGG 1 cut(s) 120
BstENI CCTNNNNNAGG 1 cut(s) 199
BstF5I GGATG 1 cut(s) 1077
BstFNI CGCG 1 cut(s) 122
BstH2I RGCGCY 3 cut(s) 991, 1289, 1310
BstHHI GCGC 4 cut(s) 415, 990, 1288, 1309
BstKTI GATC 6 cut(s) 186, 542, 573, 1015, 1320, 1324
BstMAI GTCTC 1 cut(s) 768
BstMBI GATC 6 cut(s) 183, 539, 570, 1012, 1317, 1321
BstMWI GCNNNNNNNGC 6 cut(s) 29, 38, 116, 128, 1053, 1244
BstNSI RCATGY 3 cut(s) 731, 925, 1060
BstSCI CCNGG 1 cut(s) 1223
BstSFI CTRYAG 2 cut(s) 560, 898
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 3 cut(s) 331, 402, 568
BstX2I RGATCY 1 cut(s) 539
BstYI RGATCY 1 cut(s) 539
Bsu15I ATCGAT 1 cut(s) 1320
Bsu36I CCTNAGG 1 cut(s) 167
BsuRI GGCC 1 cut(s) 447
BsuTUI ATCGAT 1 cut(s) 1320
BtgI CCRYGG 1 cut(s) 120
BtgZI GCGATG 1 cut(s) 43
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 1 cut(s) 1077
BtsIMutI CAGTG 4 cut(s) 294, 831, 953, 1060
Cac8I GCNNGC 7 cut(s) 324, 577, 608, 923, 1058, 1116, 1236
CciI TCATGA 1 cut(s) 288
CfoI GCGC 4 cut(s) 415, 990, 1288, 1309
Cfr13I GGNCC 1 cut(s) 865
Cfr42I CCGCGG 1 cut(s) 123
ClaI ATCGAT 1 cut(s) 1320
CseI GACGC 1 cut(s) 263
Csp6I GTAC 8 cut(s) 172, 273, 595, 620, 625, 829, 912, 939
CviAII CATG 8 cut(s) 280, 289, 535, 611, 694, 728, 922, 1057
CviQI GTAC 8 cut(s) 172, 273, 595, 620, 625, 829, 912, 939
DdeI CTNAG 9 cut(s) 167, 200, 235, 327, 846, 1142, 1239, 1282, 1303
DpnI GATC 6 cut(s) 185, 541, 572, 1014, 1319, 1323
DpnII GATC 6 cut(s) 183, 539, 570, 1012, 1317, 1321
DrdI GACNNNNNNGTC 2 cut(s) 428, 782
DseDI GACNNNNNNGTC 2 cut(s) 428, 782
Eam1104I CTCTTC 3 cut(s) 363, 495, 1104
EarI CTCTTC 3 cut(s) 363, 495, 1104
EciI GGCGGA 1 cut(s) 118
Ecl136II GAGCTC 1 cut(s) 110
Eco130I CCWWGG 2 cut(s) 668, 1084
Eco24I GRGCYC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 865
Eco53kI GAGCTC 1 cut(s) 110
Eco81I CCTNAGG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 110
EcoNI CCTNNNNNAGG 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 881
EcoT14I CCWWGG 2 cut(s) 668, 1084
EcoT38I GRGCYC 1 cut(s) 112
ErhI CCWWGG 2 cut(s) 668, 1084
FaeI CATG 8 cut(s) 283, 292, 538, 614, 697, 731, 925, 1060
FalI AAGNNNNNCTT 4 cut(s) 802, 834, 1002, 1034
FaqI GGGAC 3 cut(s) 226, 778, 878
FatI CATG 8 cut(s) 279, 288, 534, 610, 693, 727, 921, 1056
FauI CCCGC 1 cut(s) 1243
FbaI TGATCA 1 cut(s) 183
FblI GTMKAC 4 cut(s) 432, 465, 969, 1215
Fnu4HI GCNGC 8 cut(s) 63, 120, 123, 268, 320, 390, 416, 557
FokI GGATG 1 cut(s) 1064
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 8 cut(s) 63, 120, 123, 268, 320, 390, 416, 557
FspBI CTAG 7 cut(s) 191, 323, 407, 515, 872, 926, 1115
GlaI GCGC 4 cut(s) 414, 989, 1287, 1308
GluI GCNGC 8 cut(s) 63, 120, 123, 268, 320, 390, 416, 557
GsaI CCCAGC 1 cut(s) 765
GsuI CTGGAG 1 cut(s) 318
HaeII RGCGCY 3 cut(s) 991, 1289, 1310
HaeIII GGCC 1 cut(s) 447
HapII CCGG 1 cut(s) 1224
HgaI GACGC 1 cut(s) 263
HhaI GCGC 4 cut(s) 415, 990, 1288, 1309
Hin1I GRCGYC 1 cut(s) 1277
Hin1II CATG 8 cut(s) 283, 292, 538, 614, 697, 731, 925, 1060
Hin6I GCGC 4 cut(s) 413, 988, 1286, 1307
HinP1I GCGC 4 cut(s) 413, 988, 1286, 1307
HincII GTYRAC 5 cut(s) 400, 433, 466, 970, 1258
HindII GTYRAC 5 cut(s) 400, 433, 466, 970, 1258
HindIII AAGCTT 1 cut(s) 1245
HinfI GANTC 5 cut(s) 16, 239, 260, 614, 848
HpaII CCGG 1 cut(s) 1224
HphI GGTGA 2 cut(s) 178, 1243
Hpy166II GTNNAC 7 cut(s) 400, 433, 466, 831, 970, 1216, 1258
Hpy188I TCNGA 4 cut(s) 238, 253, 330, 1024
Hpy188III TCNNGA 7 cut(s) 9, 289, 586, 1001, 1273, 1294, 1315
Hpy8I GTNNAC 7 cut(s) 400, 433, 466, 831, 970, 1216, 1258
Hpy99I CGWCG 4 cut(s) 257, 470, 912, 971
HpyAV CCTTC 2 cut(s) 367, 1335
HpyCH4III ACNGT 9 cut(s) 364, 594, 624, 792, 860, 893, 916, 948, 1255
HpyCH4IV ACGT 2 cut(s) 423, 1277
HpyCH4V TGCA 8 cut(s) 32, 137, 283, 382, 697, 753, 1008, 1060
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 38, 116, 128, 1053, 1244
HpyF3I CTNAG 9 cut(s) 167, 200, 235, 327, 846, 1142, 1239, 1282, 1303
HpySE526I ACGT 2 cut(s) 423, 1277
Hsp92I GRCGYC 1 cut(s) 1277
Hsp92II CATG 8 cut(s) 283, 292, 538, 614, 697, 731, 925, 1060
HspAI GCGC 4 cut(s) 413, 988, 1286, 1307
Ksp22I TGATCA 1 cut(s) 183
KspI CCGCGG 1 cut(s) 123
Kzo9I GATC 6 cut(s) 183, 539, 570, 1012, 1317, 1321
LguI GCTCTTC 1 cut(s) 1104
LmnI GCTCC 5 cut(s) 299, 316, 478, 995, 1138
Lsp1109I GCAGC 3 cut(s) 331, 402, 568
LweI GCATC 6 cut(s) 14, 93, 215, 400, 643, 654
MaeI CTAG 7 cut(s) 191, 323, 407, 515, 872, 926, 1115
MaeII ACGT 2 cut(s) 423, 1277
MaeIII GTNAC 2 cut(s) 812, 1068
MalI GATC 6 cut(s) 185, 541, 572, 1014, 1319, 1323
MbiI CCGCTC 1 cut(s) 267
MboI GATC 6 cut(s) 183, 539, 570, 1012, 1317, 1321
MboII GAAGA 7 cut(s) 380, 512, 658, 673, 1121, 1240, 1344
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 2 cut(s) 112, 165
MluCI AATT 2 cut(s) 689, 881
MlyI GAGTC 3 cut(s) 248, 608, 857
MmeI TCCRAC 1 cut(s) 276
MseI TTAA 3 cut(s) 140, 452, 995
MslI CAYNNNNRTG 1 cut(s) 1040
MspA1I CMGCKG 2 cut(s) 122, 488
MspCI CTTAAG 1 cut(s) 994
MspI CCGG 1 cut(s) 1224
MspR9I CCNGG 1 cut(s) 1225
Mva1269I GAATGC 1 cut(s) 530
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 6 cut(s) 29, 38, 116, 128, 1053, 1244
NciI CCSGG 1 cut(s) 1225
NdeII GATC 6 cut(s) 183, 539, 570, 1012, 1317, 1321
NheI GCTAGC 2 cut(s) 322, 1114
NlaIII CATG 8 cut(s) 283, 292, 538, 614, 697, 731, 925, 1060
NlaIV GGNNCC 2 cut(s) 480, 866
NspI RCATGY 3 cut(s) 731, 925, 1060
NspV TTCGAA 1 cut(s) 1107
PaeI GCATGC 2 cut(s) 925, 1060
PagI TCATGA 1 cut(s) 288
PciI ACATGT 1 cut(s) 727
PciSI GCTCTTC 1 cut(s) 1104
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PctI GAATGC 1 cut(s) 530
PfeI GAWTC 2 cut(s) 16, 260
PflMI CCANNNNNTGG 2 cut(s) 443, 1135
PkrI GCNGC 8 cut(s) 64, 121, 124, 269, 321, 391, 417, 558
PleI GAGTC 3 cut(s) 247, 608, 856
PpsI GAGTC 3 cut(s) 247, 608, 856
PscI ACATGT 1 cut(s) 727
Psp124BI GAGCTC 1 cut(s) 112
PspFI CCCAGC 1 cut(s) 761
PspN4I GGNNCC 2 cut(s) 480, 866
PspPI GGNCC 1 cut(s) 865
PsuI RGATCY 1 cut(s) 539
RsaI GTAC 8 cut(s) 173, 274, 596, 621, 626, 830, 913, 940
RsaNI GTAC 8 cut(s) 172, 273, 595, 620, 625, 829, 912, 939
RseI CAYNNNNRTG 1 cut(s) 1040
SacI GAGCTC 1 cut(s) 112
SacII CCGCGG 1 cut(s) 123
SalI GTCGAC 3 cut(s) 431, 464, 968
SapI GCTCTTC 1 cut(s) 1104
SaqAI TTAA 3 cut(s) 140, 452, 995
SatI GCNGC 8 cut(s) 63, 120, 123, 268, 320, 390, 416, 557
Sau3AI GATC 6 cut(s) 183, 539, 570, 1012, 1317, 1321
Sau96I GGNCC 1 cut(s) 865
SchI GAGTC 3 cut(s) 248, 608, 857
ScrFI CCNGG 1 cut(s) 1225
SduI GDGCHC 2 cut(s) 112, 165
SfaNI GCATC 6 cut(s) 14, 93, 215, 400, 643, 654
SfcI CTRYAG 2 cut(s) 560, 898
Sfr303I CCGCGG 1 cut(s) 123
SfuI TTCGAA 1 cut(s) 1107
SgrBI CCGCGG 1 cut(s) 123
SinI GGWCC 1 cut(s) 865
SmiMI CAYNNNNRTG 1 cut(s) 1040
SmlI CTYRAG 1 cut(s) 994
SmoI CTYRAG 1 cut(s) 994
SphI GCATGC 2 cut(s) 925, 1060
Sse9I AATT 2 cut(s) 689, 881
SsiI CCGC 8 cut(s) 63, 120, 122, 129, 267, 390, 488, 1236
SspMI CTAG 7 cut(s) 191, 323, 407, 515, 872, 926, 1115
SstI GAGCTC 1 cut(s) 112
StyD4I CCNGG 1 cut(s) 1223
StyI CCWWGG 2 cut(s) 668, 1084
TaaI ACNGT 9 cut(s) 364, 594, 624, 792, 860, 893, 916, 948, 1255
TaiI ACGT 2 cut(s) 426, 1280
TaqI TCGA 8 cut(s) 263, 312, 432, 465, 840, 969, 1107, 1320
TasI AATT 2 cut(s) 689, 881
TatI WGTACW 2 cut(s) 272, 624
TauI GCSGC 5 cut(s) 65, 122, 125, 270, 392
TfiI GAWTC 2 cut(s) 16, 260
Tru1I TTAA 3 cut(s) 140, 452, 995
Tru9I TTAA 3 cut(s) 140, 452, 995
TscAI CASTG 4 cut(s) 301, 838, 953, 1067
TseI GCWGC 3 cut(s) 319, 415, 556
TspDTI ATGAA 6 cut(s) 347, 351, 523, 682, 730, 972
TspGWI ACGGA 2 cut(s) 373, 607
TspRI CASTG 4 cut(s) 301, 838, 953, 1067
Van91I CCANNNNNTGG 2 cut(s) 443, 1135
Vha464I CTTAAG 1 cut(s) 994
VpaK11BI GGWCC 1 cut(s) 865
XagI CCTNNNNNAGG 1 cut(s) 199
XapI RAATTY 2 cut(s) 689, 881
XceI RCATGY 3 cut(s) 731, 925, 1060
XmiI GTMKAC 4 cut(s) 432, 465, 969, 1215
XspI CTAG 7 cut(s) 191, 323, 407, 515, 872, 926, 1115
ZraI GACGTC 1 cut(s) 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.