pycom11g22420

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000128
Physical Location & Seq
Forward (+)
25422924 .. 25429641
6718 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g22420.7

Sequence Viewer

Length: 2781 bp
ATGTCAAAGGCTAGGCATGACTTTGCTTCCTCTTCAAATCTTGGAAAGAAGGTTTCAAACACCGTCAACGACAAAGATCGCGACCTCACCGAGACTCAAAAGAAGTTGAAAGAGCATGATTATGGAGTTGACAACAACAAGGCTGAACTTGGCTTCACACCAAATGCACCCGTGAAGATTTCAAGCAAAGTGAAGAAAGCTAGCGCTCAACACATCAGCGTGAGCATTGAACAAGACCAAGAAGAGCCTAAACCCTCCCCTCGGATGTCGGTCTTCAATAGGATGAGCCATTCAAGCTCCAGAACTTCAGTGCTTAATCGCATTGGTGGTCAAGACCGAACCTCTGTCTTCAAGAGGCTTAACGCGCCGACATCCCAAAGCTCTGTTTTTGAAAGGTTGTTAAAGCCTAAGAAACAAAGCAACACAGCTATCTCTCCTCTGCAACGATCAGCTTCAGAAATATTTGAAGATAATGGTAAGCCTTTTGGAAAGAGGAAGACAACCCCAAAGGGAGAAAAGCTCGATGGGTTAGGAGAAAAAGACGACGCTCGAAGCTTGATTCCTTCAAGGATGGAGCGCCAAGCAATCCTAGAGGTTGACACAAAAGGACCACTGAAGGTAAGGAGGCGCATCATCGTCCACACTGGCAAATCTTCATGCCAACAAACTCAAGAGAACGGCACTGAAGAGGAGATCCAAGATGTTTTCTACATCACGGTTCAAGAAGGTAAAGAAGACGAAAGCCCGAAGAAATACTCAGAGTCCCTACTCTTCGACCCTGATCCAAGCGGCAGTACAACCATGCAAGTCATGACCACTGGAGCAACTTCAATCGAGGAGCAGCTAGCTCAGATGAATGAAGTAATCGCAAAGCTCACACGGACTGTGGAAGAGAAGGACTTGCAGATTGCCGCACTTGTCAACCAACTAGATGCGCTGCCCGACGTGAAAGTCGACCCAAATATTGGCCTATTAAAGAAAGAAGACGACGAAGAAGAGGAGCCACCAGTGGAGAAAGTTGAAGAGAAGCCGAAGCTAGACCAAACAATGACATCCATGGGATCTCTCTCTATCCAGCAGCTACAGGAGATGATTGCAAGCACTATCAAGACACAGTACGAAGGAAGCTCGCACGACTCCGTACTGTACTCAAAGCCTTACTCCAAGAAGATTGATGCTTTGAAGATGCCAAGGGGTTATCAACCCCCAAAATTCATGCAGTTCGATGGAAAAGGCAACCCGAAGCAACATGTCGCCCATTTCATTGAAACTTGCAACAATGCTGGGACAGAGGGAGACTACCTCGTCAAACAGTTCGTGCGTTCGCTGAAGAGTAACGCTTTTGACTGGTACACCGACCTCGAACCCGAGTCTATCAACAGTTGGGACCAGCTAGAAAGGGAATTCCTCAACCGTTTCTACAATGAACCTGTCATCGACTACATAAATAGATGGCGTTCATTAAGCCTGGATTGCAAAGATCGGCTTTCTGAAACCTCCGCCATCGAGATGTGCGTTCAAGGCATGCAGTGGGGACTACACTACATCCTTCAAGGCATCAAACCACGAACATTTGAGGAGTTAGCCACCCGCGCCCATGATATGGAGTTAAGCATCACCCGTTATGGGAAGAAAGAGCAGATCGCCGACTACAAGAATGACAAAGTTTTGGGGCCAAAGGTGGATAAGGCTGCGTGGAAACCCACCAAGGAAGCAATGACGCTCAACACAACTCCAGTCAAAACCCATACACGAGGCAAGGCGATTCAAACCGAAGCTTTTCGTGATCAAGAGATACGTAGACGCACTTTGAAGGAGCTTGAGGAGAAGACTTATCCATTCCCCGACTCTGACGTGGTTGCCATGTTAGATGACTTGCTGGAAAAAAAGGTGATCAACCTACCTGAGTGTAAACGGCCGGAAGAGATGAACCGTACTGACAGTCCAAGGTACTGTAAATTCCACCGCTTCATTGGTCATCCGACGGAAAAGTGCTTTGTGCTAAAAGATCTCATCATGAAGTTGGCTCAGAAAGGGATCATCGAGCTAGATCTTAATGATGTGGTGAAATCAAACTATACCTCCGTCACTTCTGGCTCTTTGAACTCAAAATCTTCACCTCAACCGCTGGGGGCATGCTCCAAAACCATGTCAGTCAAGTCAAGTGAAGTTGAAGGATGGACTTATGTCACTCCAAAGAAAATGCACAAGAAACATAGGTCTCCTCCACAAGTTCACCAATCGGAAAGGGGGCAAAGCAGCTACCGTCAACCTTCAATATTACATGAAAATGTTGAGGATGATGAAATTATGACACAAAGATCGCCCGTTGCCATCACAATGGGCGATTTCTTCCCCGAAGACTTCTTCAATCACTCACTTCTACTGAGAAAGGAACAGAAGATTCGTATTCAATACGAATTGGAAAGATTGAACAAAGAAACAGGTCAGCACCTCCACCTCGTACTTGCCTGCCATATCCTGAAACCGCTCGTAGACCTTCGGGGGGAGACCAAAAGAATCCTGCTGCCCAGTTCAAGAGTAGCACAAAGGAAAATCAACGAGCGGAGGAGACCAAAAGAATCCTCCAGCCCAGTTCAAGATCAAGCCTCAATGGCCCTTGAAGAAATCACAAGTCCGATTCAAAATCAAGTGTTCACCACCCTTGAATCAAATTCAACTCCAGATAAAAGGAGTAAGTTCAGACCTTCGGAGGAGACCAAAAGAATCTTCCAAAAGAAAGAAGGGAATTCACATCACACCAAAACCTTGAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

927

Amino Acids

105.63

Weight (kDa)

8.74

Isoelectric Point (pI)

55.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 950, 1304
AccB7I CCANNNNNTGG 2 cut(s) 965, 1603
AccBSI CCGCTC 2 cut(s) 2491, 2566
AccI GTMKAC 3 cut(s) 954, 1801, 2496
AccII CGCG 3 cut(s) 81, 365, 1593
AciI CCGC 8 cut(s) 789, 912, 1500, 1591, 1966, 2126, 2489, 2566
AclWI GGATC 4 cut(s) 688, 776, 1069, 2045
AcoI YGGCCR 1 cut(s) 1916
AcsI RAATTY 5 cut(s) 1211, 1403, 1958, 2674, 2749
AcuI CTGAAG 5 cut(s) 291, 438, 635, 705, 1349
AfaI GTAC 8 cut(s) 796, 1118, 1143, 1148, 1352, 1936, 1952, 2466
AfeI AGCGCT 1 cut(s) 205
AfiI CCNNNNNNNGG 5 cut(s) 261, 965, 1603, 1626, 2506
AflIII ACRYGT 1 cut(s) 1249
AjiI CACGTC 2 cut(s) 946, 1855
AjnI CCWGG 1 cut(s) 1467
AjuI GAANNNNNNNTTGG 2 cut(s) 2714, 2746
Alw26I GTCTC 6 cut(s) 86, 1290, 2226, 2504, 2566, 2711
AlwI GGATC 4 cut(s) 688, 776, 1069, 2045
Ama87I CYCGRG 1 cut(s) 1367
Aor51HI AGCGCT 1 cut(s) 205
AoxI GGCC 4 cut(s) 967, 1673, 1916, 2616
ApeKI GCWGC 6 cut(s) 841, 937, 1078, 1691, 2259, 2527
ApoI RAATTY 5 cut(s) 1211, 1403, 1958, 2674, 2749
Asp700I GAANNNNTTC 1 cut(s) 1779
AspLEI GCGC 6 cut(s) 206, 367, 579, 630, 937, 1595
AspS9I GGNCC 4 cut(s) 608, 1387, 1673, 2617
AsuHPI GGTGA 7 cut(s) 79, 1609, 1903, 2077, 2109, 2228, 2650
AsuNHI GCTAGC 2 cut(s) 200, 844
AvaI CYCGRG 1 cut(s) 1367
AvaII GGWCC 2 cut(s) 608, 1387
BauI CACGAG 1 cut(s) 1752
BbsI GAAGAC 7 cut(s) 265, 340, 503, 741, 990, 1835, 2367
BbvI GCAGC 6 cut(s) 853, 924, 1090, 1678, 2271, 2514
BccI CCATC 7 cut(s) 518, 565, 1220, 1446, 1511, 2172, 2342
BceAI ACGGC 2 cut(s) 694, 1931
BciT130I CCWGG 1 cut(s) 1469
BclI TGATCA 2 cut(s) 1786, 1893
BcoDI GTCTC 6 cut(s) 86, 1290, 2226, 2504, 2566, 2711
BfaI CTAG 8 cut(s) 12, 201, 590, 845, 929, 1037, 1394, 2048
BfmI CTRYAG 1 cut(s) 1082
BfoI RGCGCY 2 cut(s) 207, 580
BglI GCCNNNNNGGC 1 cut(s) 2615
BglII AGATCT 2 cut(s) 2008, 2050
BisI GCNGC 8 cut(s) 790, 842, 912, 938, 1079, 1692, 2260, 2528
BlsI GCNGC 8 cut(s) 791, 843, 913, 939, 1080, 1693, 2261, 2529
Bme1390I CCNGG 1 cut(s) 1469
Bme18I GGWCC 2 cut(s) 608, 1387
BmeT110I CYCGRG 1 cut(s) 1367
BmgBI CACGTC 2 cut(s) 946, 1855
BmgT120I GGNCC 4 cut(s) 608, 1387, 1673, 2617
BmiI GGNNCC 3 cut(s) 1002, 1388, 1674
BmrFI CCNGG 1 cut(s) 1469
BmrI ACTGGG 2 cut(s) 2526, 2588
BmsI GCATC 6 cut(s) 639, 922, 1165, 1176, 1566, 1623
BmtI GCTAGC 2 cut(s) 204, 848
BmuI ACTGGG 2 cut(s) 2526, 2588
BoxI GACNNNNGTC 1 cut(s) 2186
BpiI GAAGAC 7 cut(s) 265, 340, 503, 741, 990, 1835, 2367
BplI GAGNNNNNCTC 4 cut(s) 504, 536, 1287, 1319
BpmI CTGGAG 5 cut(s) 283, 840, 1719, 2572, 2667
BpuEI CTTGAG 2 cut(s) 654, 1841
BsaAI YACGTR 1 cut(s) 1799
BsaI GGTCTC 4 cut(s) 2226, 2504, 2566, 2711
BsaJI CCNNGG 5 cut(s) 260, 1056, 1190, 1707, 1946
BsaXI ACNNNNNCTCC 2 cut(s) 2066, 2096
Bsc4I CCNNNNNNNGG 5 cut(s) 261, 965, 1603, 1626, 2506
Bse1I ACTGG 7 cut(s) 649, 823, 1007, 1352, 1736, 2532, 2594
Bse3DI GCAATG 1 cut(s) 1722
BseBI CCWGG 1 cut(s) 1469
BseDI CCNNGG 5 cut(s) 260, 1056, 1190, 1707, 1946
BseGI GGATG 9 cut(s) 270, 288, 371, 576, 1052, 1545, 1978, 2183, 2305
BseLI CCNNNNNNNGG 5 cut(s) 261, 965, 1603, 1626, 2506
BseMI GCAATG 1 cut(s) 1722
BseMII CTCAG 5 cut(s) 771, 863, 1896, 2042, 2378
BseNI ACTGG 7 cut(s) 649, 823, 1007, 1352, 1736, 2532, 2594
BseRI GAGGAG 9 cut(s) 426, 704, 851, 1013, 1592, 1838, 2214, 2584, 2729
BseX3I CGGCCG 1 cut(s) 1916
BseXI GCAGC 6 cut(s) 853, 924, 1090, 1678, 2271, 2514
BseYI CCCAGC 2 cut(s) 1283, 2128
Bsh1236I CGCG 3 cut(s) 81, 365, 1593
Bsh1285I CGRYCG 1 cut(s) 1919
BshFI GGCC 4 cut(s) 969, 1675, 1918, 2618
BsiEI CGRYCG 1 cut(s) 1919
BsiHKCI CYCGRG 1 cut(s) 1367
BsiSI CCGG 1 cut(s) 1919
BslFI GGGAC 4 cut(s) 748, 1300, 1400, 1548
BslI CCNNNNNNNGG 5 cut(s) 261, 965, 1603, 1626, 2506
BsmAI GTCTC 6 cut(s) 86, 1290, 2226, 2504, 2566, 2711
BsmFI GGGAC 4 cut(s) 748, 1300, 1400, 1548
BsnI GGCC 4 cut(s) 969, 1675, 1918, 2618
Bso31I GGTCTC 4 cut(s) 2226, 2504, 2566, 2711
BsoBI CYCGRG 1 cut(s) 1367
Bsp19I CCATGG 1 cut(s) 1056
Bsp68I TCGCGA 1 cut(s) 81
BspACI CCGC 8 cut(s) 789, 912, 1500, 1591, 1966, 2126, 2489, 2566
BspANI GGCC 4 cut(s) 969, 1675, 1918, 2618
BspCNI CTCAG 5 cut(s) 770, 862, 1897, 2041, 2379
BspFNI CGCG 3 cut(s) 81, 365, 1593
BspHI TCATGA 2 cut(s) 810, 2016
BspLI GGNNCC 3 cut(s) 1002, 1388, 1674
BspOI GCTAGC 2 cut(s) 204, 848
BspPI GGATC 4 cut(s) 688, 776, 1069, 2045
BspQI GCTCTTC 1 cut(s) 237
BspTNI GGTCTC 4 cut(s) 2226, 2504, 2566, 2711
BsrBI CCGCTC 2 cut(s) 2491, 2566
BsrDI GCAATG 1 cut(s) 1722
BsrI ACTGG 7 cut(s) 649, 823, 1007, 1352, 1736, 2532, 2594
BssECI CCNNGG 5 cut(s) 260, 1056, 1190, 1707, 1946
BssSI CACGAG 1 cut(s) 1752
BssT1I CCWWGG 4 cut(s) 1056, 1190, 1707, 1946
Bst2BI CACGAG 1 cut(s) 1752
Bst2UI CCWGG 1 cut(s) 1469
Bst6I CTCTTC 9 cut(s) 37, 237, 681, 776, 885, 990, 1017, 1325, 1917
BstBAI YACGTR 1 cut(s) 1799
BstC8I GCNNGC 7 cut(s) 202, 846, 1099, 1130, 1526, 2137, 2473
BstDEI CTNAG 6 cut(s) 408, 757, 849, 1905, 2028, 2387
BstDSI CCRYGG 1 cut(s) 1056
BstF5I GGATG 9 cut(s) 270, 288, 371, 576, 1052, 1545, 1978, 2183, 2305
BstFNI CGCG 3 cut(s) 81, 365, 1593
BstH2I RGCGCY 2 cut(s) 207, 580
BstHHI GCGC 6 cut(s) 206, 367, 579, 630, 937, 1595
BstMAI GTCTC 6 cut(s) 86, 1290, 2226, 2504, 2566, 2711
BstMCI CGRYCG 1 cut(s) 1919
BstMWI GCNNNNNNNGC 6 cut(s) 294, 364, 1473, 1521, 1592, 2615
BstNI CCWGG 1 cut(s) 1469
BstNSI RCATGY 3 cut(s) 1253, 1528, 2139
BstPAI GACNNNNGTC 1 cut(s) 2186
BstSCI CCNGG 1 cut(s) 1467
BstSFI CTRYAG 1 cut(s) 1082
BstSNI TACGTA 1 cut(s) 1799
BstUI CGCG 3 cut(s) 81, 365, 1593
BstV1I GCAGC 6 cut(s) 853, 924, 1090, 1678, 2271, 2514
BstV2I GAAGAC 7 cut(s) 265, 340, 503, 741, 990, 1835, 2367
BstX2I RGATCY 4 cut(s) 693, 1061, 2008, 2050
BstXI CCANNNNNNTGG 1 cut(s) 2341
BstYI RGATCY 4 cut(s) 693, 1061, 2008, 2050
BstZI CGGCCG 1 cut(s) 1916
BsuRI GGCC 4 cut(s) 969, 1675, 1918, 2618
BtgI CCRYGG 1 cut(s) 1056
BtrI CACGTC 2 cut(s) 946, 1855
BtsCI GGATG 9 cut(s) 270, 288, 371, 576, 1052, 1545, 1978, 2183, 2305
BtsI GCAGTG 1 cut(s) 1535
BtsIMutI CAGTG 7 cut(s) 315, 611, 642, 681, 816, 1014, 1535
BtuMI TCGCGA 1 cut(s) 81
Cac8I GCNNGC 7 cut(s) 202, 846, 1099, 1130, 1526, 2137, 2473
CciI TCATGA 2 cut(s) 810, 2016
CfoI GCGC 6 cut(s) 206, 367, 579, 630, 937, 1595
Cfr13I GGNCC 4 cut(s) 608, 1387, 1673, 2617
CseI GACGC 3 cut(s) 554, 1729, 1812
Csp6I GTAC 8 cut(s) 795, 1117, 1142, 1147, 1351, 1935, 1951, 2465
CviQI GTAC 8 cut(s) 795, 1117, 1142, 1147, 1351, 1935, 1951, 2465
DdeI CTNAG 6 cut(s) 408, 757, 849, 1905, 2028, 2387
DrdI GACNNNNNNGTC 2 cut(s) 950, 1304
DseDI GACNNNNNNGTC 2 cut(s) 950, 1304
EaeI YGGCCR 1 cut(s) 1916
EagI CGGCCG 1 cut(s) 1916
Eam1104I CTCTTC 9 cut(s) 37, 237, 681, 776, 885, 990, 1017, 1325, 1917
EarI CTCTTC 9 cut(s) 37, 237, 681, 776, 885, 990, 1017, 1325, 1917
EciI GGCGGA 1 cut(s) 1489
EclXI CGGCCG 1 cut(s) 1916
Eco105I TACGTA 1 cut(s) 1799
Eco130I CCWWGG 4 cut(s) 1056, 1190, 1707, 1946
Eco31I GGTCTC 4 cut(s) 2226, 2504, 2566, 2711
Eco47I GGWCC 2 cut(s) 608, 1387
Eco47III AGCGCT 1 cut(s) 205
Eco52I CGGCCG 1 cut(s) 1916
Eco57I CTGAAG 5 cut(s) 291, 438, 635, 705, 1349
Eco88I CYCGRG 1 cut(s) 1367
EcoRI GAATTC 2 cut(s) 1403, 2749
EcoRII CCWGG 1 cut(s) 1467
EcoT14I CCWWGG 4 cut(s) 1056, 1190, 1707, 1946
ErhI CCWWGG 4 cut(s) 1056, 1190, 1707, 1946
FalI AAGNNNNNCTT 2 cut(s) 1470, 1502
FaqI GGGAC 4 cut(s) 748, 1300, 1400, 1548
FauI CCCGC 1 cut(s) 1598
FbaI TGATCA 2 cut(s) 1786, 1893
FblI GTMKAC 3 cut(s) 954, 1801, 2496
Fnu4HI GCNGC 8 cut(s) 790, 842, 912, 938, 1079, 1692, 2260, 2528
FokI GGATG 9 cut(s) 277, 295, 358, 583, 1039, 1532, 1965, 2190, 2312
Fsp4HI GCNGC 8 cut(s) 790, 842, 912, 938, 1079, 1692, 2260, 2528
FspBI CTAG 8 cut(s) 12, 201, 590, 845, 929, 1037, 1394, 2048
GlaI GCGC 6 cut(s) 205, 366, 578, 629, 936, 1594
GluI GCNGC 8 cut(s) 790, 842, 912, 938, 1079, 1692, 2260, 2528
GsaI CCCAGC 2 cut(s) 1287, 2132
GsuI CTGGAG 5 cut(s) 283, 840, 1719, 2572, 2667
HaeII RGCGCY 2 cut(s) 207, 580
HaeIII GGCC 4 cut(s) 969, 1675, 1918, 2618
HapII CCGG 1 cut(s) 1919
HgaI GACGC 3 cut(s) 554, 1729, 1812
HhaI GCGC 6 cut(s) 206, 367, 579, 630, 937, 1595
Hin6I GCGC 6 cut(s) 204, 365, 577, 628, 935, 1593
HinP1I GCGC 6 cut(s) 204, 365, 577, 628, 935, 1593
HincII GTYRAC 6 cut(s) 67, 130, 598, 922, 955, 2270
HindII GTYRAC 6 cut(s) 67, 130, 598, 922, 955, 2270
HindIII AAGCTT 2 cut(s) 553, 1776
HpaII CCGG 1 cut(s) 1919
HphI GGTGA 7 cut(s) 79, 1609, 1903, 2077, 2109, 2228, 2650
Hpy99I CGWCG 4 cut(s) 548, 947, 992, 1987
HpyCH4IV ACGT 3 cut(s) 945, 1798, 1854
HpyF10VI GCNNNNNNNGC 6 cut(s) 294, 364, 1473, 1521, 1592, 2615
HpyF3I CTNAG 6 cut(s) 408, 757, 849, 1905, 2028, 2387
HpySE526I ACGT 3 cut(s) 945, 1798, 1854
HspAI GCGC 6 cut(s) 204, 365, 577, 628, 935, 1593
Ksp22I TGATCA 2 cut(s) 1786, 1893
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 7 cut(s) 302, 574, 821, 838, 1000, 1816, 2144
Lsp1109I GCAGC 6 cut(s) 853, 924, 1090, 1678, 2271, 2514
LweI GCATC 6 cut(s) 639, 922, 1165, 1176, 1566, 1623
MaeI CTAG 8 cut(s) 12, 201, 590, 845, 929, 1037, 1394, 2048
MaeII ACGT 3 cut(s) 945, 1798, 1854
MaeIII GTNAC 3 cut(s) 1334, 2086, 2188
MbiI CCGCTC 2 cut(s) 2491, 2566
MflI RGATCY 4 cut(s) 693, 1061, 2008, 2050
MluCI AATT 7 cut(s) 1211, 1403, 1958, 2307, 2420, 2674, 2749
MlyI GAGTC 5 cut(s) 88, 770, 1130, 1379, 1841
MmeI TCCRAC 1 cut(s) 2006
MroXI GAANNNNTTC 1 cut(s) 1779
MseI TTAA 7 cut(s) 315, 360, 401, 974, 1463, 1610, 2055
MslI CAYNNNNRTG 5 cut(s) 120, 218, 1508, 2289, 2339
MspA1I CMGCKG 1 cut(s) 2128
MspI CCGG 1 cut(s) 1919
MspR9I CCNGG 1 cut(s) 1469
MvaI CCWGG 1 cut(s) 1469
MvnI CGCG 3 cut(s) 81, 365, 1593
MwoI GCNNNNNNNGC 6 cut(s) 294, 364, 1473, 1521, 1592, 2615
NcoI CCATGG 1 cut(s) 1056
NheI GCTAGC 2 cut(s) 200, 844
NlaIV GGNNCC 3 cut(s) 1002, 1388, 1674
NmuCI GTSAC 2 cut(s) 2086, 2188
NruI TCGCGA 1 cut(s) 81
NspI RCATGY 3 cut(s) 1253, 1528, 2139
PaeI GCATGC 2 cut(s) 1528, 2139
PagI TCATGA 2 cut(s) 810, 2016
PciI ACATGT 1 cut(s) 1249
PciSI GCTCTTC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 2 cut(s) 951, 1512
PdmI GAANNNNTTC 1 cut(s) 1779
PfeI GAWTC 8 cut(s) 559, 1765, 2404, 2520, 2582, 2641, 2669, 2727
PflMI CCANNNNNTGG 2 cut(s) 965, 1603
PkrI GCNGC 8 cut(s) 791, 843, 913, 939, 1080, 1693, 2261, 2529
PleI GAGTC 5 cut(s) 88, 769, 1130, 1378, 1841
PpsI GAGTC 5 cut(s) 88, 769, 1130, 1378, 1841
Ppu21I YACGTR 1 cut(s) 1799
PscI ACATGT 1 cut(s) 1249
PshAI GACNNNNGTC 1 cut(s) 2186
Psp6I CCWGG 1 cut(s) 1467
PspFI CCCAGC 2 cut(s) 1283, 2128
PspGI CCWGG 1 cut(s) 1467
PspN4I GGNNCC 3 cut(s) 1002, 1388, 1674
PspPI GGNCC 4 cut(s) 608, 1387, 1673, 2617
PsuI RGATCY 4 cut(s) 693, 1061, 2008, 2050
RruI TCGCGA 1 cut(s) 81
RsaI GTAC 8 cut(s) 796, 1118, 1143, 1148, 1352, 1936, 1952, 2466
RsaNI GTAC 8 cut(s) 795, 1117, 1142, 1147, 1351, 1935, 1951, 2465
RseI CAYNNNNRTG 5 cut(s) 120, 218, 1508, 2289, 2339
SalI GTCGAC 1 cut(s) 953
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 7 cut(s) 315, 360, 401, 974, 1463, 1610, 2055
SatI GCNGC 8 cut(s) 790, 842, 912, 938, 1079, 1692, 2260, 2528
Sau96I GGNCC 4 cut(s) 608, 1387, 1673, 2617
SchI GAGTC 5 cut(s) 88, 770, 1130, 1379, 1841
ScrFI CCNGG 1 cut(s) 1469
SfaNI GCATC 6 cut(s) 639, 922, 1165, 1176, 1566, 1623
SfcI CTRYAG 1 cut(s) 1082
SinI GGWCC 2 cut(s) 608, 1387
SmiMI CAYNNNNRTG 5 cut(s) 120, 218, 1508, 2289, 2339
SmlI CTYRAG 2 cut(s) 669, 1820
SmoI CTYRAG 2 cut(s) 669, 1820
SnaBI TACGTA 1 cut(s) 1799
SphI GCATGC 2 cut(s) 1528, 2139
Sse9I AATT 7 cut(s) 1211, 1403, 1958, 2307, 2420, 2674, 2749
SsiI CCGC 8 cut(s) 789, 912, 1500, 1591, 1966, 2126, 2489, 2566
SspI AATATT 3 cut(s) 462, 964, 2280
SspMI CTAG 8 cut(s) 12, 201, 590, 845, 929, 1037, 1394, 2048
StyD4I CCNGG 1 cut(s) 1467
StyI CCWWGG 4 cut(s) 1056, 1190, 1707, 1946
TaiI ACGT 3 cut(s) 948, 1801, 1857
TaqII GACCGA 2 cut(s) 259, 351
TasI AATT 7 cut(s) 1211, 1403, 1958, 2307, 2420, 2674, 2749
TatI WGTACW 2 cut(s) 794, 1146
TauI GCSGC 2 cut(s) 792, 914
TfiI GAWTC 8 cut(s) 559, 1765, 2404, 2520, 2582, 2641, 2669, 2727
Tru1I TTAA 7 cut(s) 315, 360, 401, 974, 1463, 1610, 2055
Tru9I TTAA 7 cut(s) 315, 360, 401, 974, 1463, 1610, 2055
TscAI CASTG 7 cut(s) 315, 618, 649, 688, 823, 1014, 1535
TseFI GTSAC 2 cut(s) 2086, 2188
TseI GCWGC 6 cut(s) 841, 937, 1078, 1691, 2259, 2527
Tsp45I GTSAC 2 cut(s) 2086, 2188
TspGWI ACGGA 4 cut(s) 895, 1129, 2000, 2074
TspRI CASTG 7 cut(s) 315, 618, 649, 688, 823, 1014, 1535
Van91I CCANNNNNTGG 2 cut(s) 965, 1603
VpaK11BI GGWCC 2 cut(s) 608, 1387
XapI RAATTY 5 cut(s) 1211, 1403, 1958, 2674, 2749
XceI RCATGY 3 cut(s) 1253, 1528, 2139
XcmI CCANNNNNNNNNTGG 1 cut(s) 1970
XmiI GTMKAC 3 cut(s) 954, 1801, 2496
XmnI GAANNNNTTC 1 cut(s) 1779
XspI CTAG 8 cut(s) 12, 201, 590, 845, 929, 1037, 1394, 2048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.