FvH4_7g03661

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000128
Physical Location & Seq
Forward (+)
4144640 .. 4148867
4228 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03661.t1

Sequence Viewer

Length: 3729 bp
ATGGCGAGAACTCAACCCACGACATTTGTGGCTGTACCTTCAAAGCCTCGACAACCAATTATCACCTTGGAGAGTTTAGGGGCAAGCCAACATGTCTCTCGAAGCGAAGAGGGAGAGACTCCGAAGAAATCAAGGGAGCGATCATTCTCACCCGCTTACTCTGATGGTGAATCAAGCACGGCTTCGCATCATGGGAGCCCCAATGGAGATGAAGACCATACTTATGGCATGCCATCAAGTAGCTCGTCTCACCCTATCAACATGCAAGTCATGGTGACCGGAGCAGCCACCATTGAAGAGCAAATAGCTAATCTGATGGGAGCAGTCGAGAAGCTCACCAAAACGAACAACGATGGCTCACTTGCATCCATGTCGTTCCAGCAGTTGCAAGATATCATCACCAACTCCATCAGAGCTCAATATGGAGGCCCTTCTCGTGATTCTCTCGTGTACTCCAAACCCTACACCAAGAGGATTGACAATTTAAGGATGCCGTTCATATATCAGCCACCGAAGTTCCAACAATTCGAGGGAAAAGGAAACCCGAAGCAGCATGGAAACGCCTTTGAATGGTACACAGACTTGGAACCTGAGTCTATTAACAGTTGGGACCAAATGGAGCGCGAGTTCCTAAACTGTTTCTATAGTACAAGGCGTATCGTGAGCATGATGGAACTCACCAACACAAAACAACGAGGAGGTGAGCCAGCAATCGACTTCATCAATAGGTGGCGGTCACTCAGTCTCGATTGCAAGGATCGCTTGTCAGAAGTTTTCGCGGTTGAGATGTGCATCCAAGGTATGCATTTTGACCTACTATACATTTTGCAAGGAATCAAGCCTCGGAATTTCGAAGAATTGGCTACTCGAGCACATGATATGGAGCTAAGCCTAGCTAGCCACAAGGCGAAGGAGTCTATCATTGACCAACAAAAAGACAAGATCTTCGGAAGCAAGGTTGACAAAACCGCGAAGAAACCAACGAAGGAGTCAATGGCTGTGAATGTCGCTCCAATCAAAATCTCAAACAAGAAAGAGGTTAAGAAAGTTGAGCCAACTCGCGCTTATGATAGAAGAAAACGCTCATTAAGCGAGTTGGAGCGAAAGACATATCCGTTCCTAGATACCGACGTGGCAGGCATGTTGGAAGATTTTCTTGAAAACAAGATGATAGAACTTCCGGAGTGCAAACGTCCAGAAGAAATCCATCAAGTTAATGACCCAAAATATTGCAAGTATCATCGAATCATTAGCCACCCCGTCGAGAAATGCTTTGTCTTGAAGGATTTGATAATGAACCTCACTAGACAAGGAAGAATTGAGCTCAATGTTGATGAAGTTGCTGCGGTCAATTGCACCACCATTTCTTTTGGGTCATTTGATTCGATTGAAGTACCTTCCACACCAATGAAAGCGCCATACCACTTCTCAAGAGAGGATGCCAAAGGCGAGAAGTTGGCGGAAGTTAAGCAAGTCCAACAGGTGAATCGCGCTCCTAAGGTTGTTGCTAAAACTGACCAAGGCATTGATGATGAAGGATGGATACTTGTTACTCGACGGAGAGCAGGCAAAAATGTGTCATCAGGTCCACCACCCATGCAGTTAAACTGCCAAAAACTACGTAAGAATGGTCAAAGTCTTGAGAAGAACGAGACAATGTGTGTTAAGGGAAAGACCACTCCTTGCAAGCCGAGACCTATCAGTCAAACTTTGCCAATAAAGCACATGCCTATGGCACAAACAAGTATAAGAGTTAGTTCAATGACAACAAGTTGCGAAGGTAGTTCTAAAGATTCTGAGAAGGCTGAAGCAGACAAGGTGAAAACAGATCAAGAAGCGGATGCAAATGAGGTGTTGAAACATTTGGAAGACTTACCTCTAAAACTTAGTCTCTCAGACATGTTGCAGCTACCCAAAGCAATGAGATGCACATTAGTCCAATTGTTACTCGACATGGGCAAACATTCCTCGAACATCCAAGAATGTGCGACATACGTTGCATGTTGCGATGCCGTCCATTTCACGGATGAAGACCTGCAATTGGGTTCTAAGCCTCACAATAGACCTCTTTTTGTGACAGGGTACATACGAGAGCAGAGATTGAATCGCATGCTCATAGATGGAGGGTCTGCCGTAAACATTATGCCAAAAACAACAATGTCGAGACTCGGCATCAACATTGATGAGTTGTCTAAAAGTCGTCTTATGATCCAAGGCTTCAACCAAGGAGGGCAAAGAGCAATAGGCATGATTCGATTAGACATGGACATTGCAGAATTGAAGTCAAACACCTTGTACCATGTGATTGATGCAAAGACGTCATACAATCTATTGTTGGGGCGGCCATGGGTTCATGAAAATGGTGTCGTGCCCTCAACTTTGCACCAATGTTTCAAATTCTATCGCGGAGGTGTGAAAACGGTGGTAAGGGATACCAAGCCGTTCACTGAAGCCGAGTCTTACTTTGCAGATTCCAAGTTCTATACGGATGAGGAATCGGTATCAGAAGTTCTTCCAGTTGAAGTCCCCTCAACAGGAAAAGCCACCGAGTTAAAGAAGAAAGAAGCTGAAACTGAAACTGAAAGCAAAACAAACCAAGAGTCGCATAAATCTTCATCAGAAATTACAACATCAAATGCGAAAGAGAAATCCAATCAAGCTGTCCCAATCCTTCGCTATGTGCCAAAATCCAAGCGAAAGGAAGGAGAATCTCCATTTTTGAGCGGCCAAACTCCTACCAAGCAACCGTTGAAGGGGTTTGTCAAACCAAACCAAGGCAAAATTGAGCATGGGCCCTTACCCGAAAAAAGAACAAATGAAGGGTTCGACTCCAATGCTTACAAGTTGTTGGGAAAGGCCGGGTTTGACTTCGGTTCACCAAGCAAAGAAGATGACCAAATTATTAGCAAGAAAGCACACGAGCTTAATGAGTCTCAAAAGAAGCTGAGAGAGCAAGGAAACTCAGCAAGAACAGGTCTTGGTTTTGCCCCAAGCAAGCCAATCAAGATCTCTGGAAGGAGAAAGGGTGCTCAAGCTAGCACACAATACATTGGTGTTGAAGAAGTAGAAGATGACGTTCCGCAATGTCACCATATCTCTCGCACTTCGACATTTGATCGTATCTGCCCAAGTTCTCAAAATACTACTACCGAATCAACAAGCAGAGTTTCGGTGTTTGAGCGTGTTGATAGGTCAACAAGTCGAAGCTCAGTCTTCAATCGTCTTGACATCTCAGCTACACGAATTTCAGTGTTCAAAAGTCTCATCCCATCACGCATGAAAAGACGTTGCATGTTGGAGGTGAATTCGACGGACCAACTTAAAGTGAAGCAACGCACCATCATACTCACTGGTCAAGACAAAGAAGGCGATGGTACTAGTGACGATGAATGTGATATTGTTCAATGTGCTTATCACATCACTGTAGCGGAAGCGGAAGCTTATGAAGAGGATGACCATGAGGGCTCCGAATATGAAGTGGAGGCAGCTCCTCCAGAACTTGAAGATGGGGTGAAAGCTACAGTTGATGAACTTAAGGAGCTCAATTTGGGGACAGAGGAAGACCCAAGACCAATATTTGTGAGCGCATCACTAAGTCCCCAAGAAGAGAAGGAGTACTATGACTTGTTGCTCGAATATAAGGATGTCTTCGCTTGGAATTACAAAGAAATGCCAGGTCTTGACCCTAAGAGGTGCAAACCCAAATTGAAGCAGAGGTTGACAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 Retrotrans_gag

Protein Analysis

1243

Amino Acids

139.69

Weight (kDa)

7.0

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 186 - 269 8.4e-09 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1701
AatII GACGTC 1 cut(s) 2321
Acc36I ACCTGC 1 cut(s) 2043
AccBSI CCGCTC 1 cut(s) 2724
AccII CGCG 6 cut(s) 624, 779, 971, 1062, 1491, 2406
AccIII TCCGGA 1 cut(s) 1180
AclWI GGATC 2 cut(s) 765, 2203
AcoI YGGCCR 2 cut(s) 2342, 2725
AcsI RAATTY 4 cut(s) 847, 2396, 3243, 3304
AcuI CTGAAG 2 cut(s) 1827, 2469
AcyI GRCGYC 1 cut(s) 2318
AfaI GTAC 9 cut(s) 36, 452, 575, 649, 1395, 2084, 2297, 3376, 3617
AfiI CCNNNNNNNGG 6 cut(s) 570, 2023, 2041, 2534, 2753, 2774
AflII CTTAAG 1 cut(s) 3533
AflIII ACRYGT 2 cut(s) 91, 1899
AhlI ACTAGT 1 cut(s) 3377
AjiI CACGTC 1 cut(s) 1132
AjnI CCWGG 1 cut(s) 3673
AloI GAACNNNNNNTCC 2 cut(s) 611, 643
Alw21I GWGCWC 5 cut(s) 418, 874, 1326, 3031, 3543
AlwI GGATC 2 cut(s) 765, 2203
Ama87I CYCGRG 1 cut(s) 867
Aor13HI TCCGGA 1 cut(s) 1180
AoxI GGCC 5 cut(s) 427, 2342, 2725, 2792, 2856
ApaI GGGCCC 1 cut(s) 2796
ApeKI GCWGC 5 cut(s) 284, 550, 1343, 1906, 3485
ApoI RAATTY 4 cut(s) 847, 2396, 3243, 3304
ArsI GACNNNNNNTTYG 4 cut(s) 929, 961, 2585, 2617
Asp700I GAANNNNTTC 2 cut(s) 1152, 2511
AspLEI GCGC 5 cut(s) 624, 1064, 1417, 1493, 3587
AspS9I GGNCC 6 cut(s) 428, 610, 1586, 2792, 2793, 3313
AsuC2I CCSGG 1 cut(s) 2860
AsuII TTCGAA 1 cut(s) 852
AsuNHI GCTAGC 2 cut(s) 896, 3035
AvaI CYCGRG 1 cut(s) 867
AvaII GGWCC 3 cut(s) 610, 1586, 3313
AxyI CCTNAGG 1 cut(s) 1497
BaeGI GKGCMC 2 cut(s) 2373, 2796
BaeI ACNNNNGTAYC 2 cut(s) 1116, 1149
BanII GRGCYC 6 cut(s) 200, 418, 1326, 2796, 3467, 3543
BarI GAAGNNNNNNTAC 2 cut(s) 1527, 1559
BauI CACGAG 3 cut(s) 435, 446, 2918
BbsI GAAGAC 6 cut(s) 219, 1875, 2037, 3205, 3567, 3640
Bbv12I GWGCWC 5 cut(s) 418, 874, 1326, 3031, 3543
BbvI GCAGC 5 cut(s) 296, 562, 1330, 1918, 3497
BceAI ACGGC 5 cut(s) 195, 478, 1997, 2117, 2425
BcgI CGANNNNNNTGC 4 cut(s) 354, 388, 2816, 2850
BciT130I CCWGG 1 cut(s) 3675
BciVI GTATCC 2 cut(s) 1536, 2425
BcnI CCSGG 1 cut(s) 2860
BcuI ACTAGT 1 cut(s) 3377
BfaI CTAG 6 cut(s) 893, 897, 1121, 1305, 3036, 3378
BfmI CTRYAG 3 cut(s) 643, 3423, 3519
BfoI RGCGCY 1 cut(s) 1418
BfrI CTTAAG 1 cut(s) 3533
BfuAI ACCTGC 1 cut(s) 2043
BfuI GTATCC 2 cut(s) 1536, 2425
BglII AGATCT 2 cut(s) 942, 3006
BisI GCNGC 7 cut(s) 285, 551, 1344, 1907, 2342, 2725, 3486
BlpI GCTNAGC 1 cut(s) 887
BlsI GCNGC 7 cut(s) 286, 552, 1345, 1908, 2343, 2726, 3487
BmcAI AGTACT 1 cut(s) 3617
Bme1390I CCNGG 2 cut(s) 2860, 3675
Bme18I GGWCC 3 cut(s) 610, 1586, 3313
BmeT110I CYCGRG 1 cut(s) 867
BmgBI CACGTC 1 cut(s) 1132
BmgT120I GGNCC 6 cut(s) 428, 610, 1586, 2792, 2793, 3313
BmiI GGNNCC 5 cut(s) 197, 588, 611, 2794, 3466
BmrFI CCNGG 2 cut(s) 2860, 3675
BmtI GCTAGC 2 cut(s) 900, 3039
BpiI GAAGAC 6 cut(s) 219, 1875, 2037, 3205, 3567, 3640
BpmI CTGGAG 1 cut(s) 3477
Bpu1102I GCTNAGC 1 cut(s) 887
Bpu14I TTCGAA 1 cut(s) 852
BpuEI CTTGAG 3 cut(s) 1414, 1661, 3015
BpuMI CCSGG 1 cut(s) 2860
BsaAI YACGTR 1 cut(s) 1622
BsaBI GATNNNNATC 1 cut(s) 791
BsaHI GRCGYC 1 cut(s) 2318
BsaI GGTCTC 1 cut(s) 1687
BsaJI CCNNGG 8 cut(s) 66, 796, 842, 1519, 2212, 2224, 2345, 2773
BsaWI WCCGGW 2 cut(s) 278, 1180
BsaXI ACNNNNNCTCC 4 cut(s) 611, 641, 3010, 3040
Bsc4I CCNNNNNNNGG 6 cut(s) 570, 2023, 2041, 2534, 2753, 2774
Bse1I ACTGG 2 cut(s) 2516, 3355
Bse21I CCTNAGG 1 cut(s) 1497
Bse3DI GCAATG 3 cut(s) 1926, 2268, 3089
Bse8I GATNNNNATC 1 cut(s) 791
BseAI TCCGGA 1 cut(s) 1180
BseBI CCWGG 1 cut(s) 3675
BseDI CCNNGG 8 cut(s) 66, 796, 842, 1519, 2212, 2224, 2345, 2773
BseJI GATNNNNATC 1 cut(s) 791
BseLI CCNNNNNNNGG 6 cut(s) 570, 2023, 2041, 2534, 2753, 2774
BseMI GCAATG 3 cut(s) 1926, 2268, 3089
BseMII CTCAG 8 cut(s) 582, 754, 1788, 1908, 2936, 2976, 3222, 3246
BseNI ACTGG 2 cut(s) 2516, 3355
BseRI GAGGAG 2 cut(s) 711, 3480
BseSI GKGCMC 2 cut(s) 2373, 2796
BseXI GCAGC 5 cut(s) 296, 562, 1330, 1918, 3497
Bsh1236I CGCG 6 cut(s) 624, 779, 971, 1062, 1491, 2406
BshFI GGCC 5 cut(s) 429, 2344, 2727, 2794, 2858
BsiHKAI GWGCWC 5 cut(s) 418, 874, 1326, 3031, 3543
BsiHKCI CYCGRG 1 cut(s) 867
BsiSI CCGG 3 cut(s) 279, 1181, 2859
BslFI GGGAC 5 cut(s) 623, 2510, 2648, 3565, 3582
BslI CCNNNNNNNGG 6 cut(s) 570, 2023, 2041, 2534, 2753, 2774
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 5 cut(s) 623, 2510, 2648, 3565, 3582
BsnI GGCC 5 cut(s) 429, 2344, 2727, 2794, 2858
Bso31I GGTCTC 1 cut(s) 1687
BsoBI CYCGRG 1 cut(s) 867
Bsp119I TTCGAA 1 cut(s) 852
Bsp120I GGGCCC 1 cut(s) 2792
Bsp1286I GDGCHC 9 cut(s) 200, 418, 874, 1326, 2373, 2796, 3031, 3467, 3543
Bsp13I TCCGGA 1 cut(s) 1180
Bsp143I GATC 7 cut(s) 140, 757, 942, 1828, 2208, 3006, 3115
Bsp1720I GCTNAGC 1 cut(s) 887
Bsp19I CCATGG 1 cut(s) 2345
BspANI GGCC 5 cut(s) 429, 2344, 2727, 2794, 2858
BspCNI CTCAG 8 cut(s) 583, 753, 1789, 1907, 2937, 2975, 3221, 3245
BspEI TCCGGA 1 cut(s) 1180
BspFNI CGCG 6 cut(s) 624, 779, 971, 1062, 1491, 2406
BspHI TCATGA 1 cut(s) 2353
BspLI GGNNCC 5 cut(s) 197, 588, 611, 2794, 3466
BspMI ACCTGC 1 cut(s) 2043
BspOI GCTAGC 2 cut(s) 900, 3039
BspPI GGATC 2 cut(s) 765, 2203
BspQI GCTCTTC 1 cut(s) 291
BspT104I TTCGAA 1 cut(s) 852
BspTI CTTAAG 1 cut(s) 3533
BspTNI GGTCTC 1 cut(s) 1687
BsrBI CCGCTC 1 cut(s) 2724
BsrDI GCAATG 3 cut(s) 1926, 2268, 3089
BsrI ACTGG 2 cut(s) 2516, 3355
BssECI CCNNGG 8 cut(s) 66, 796, 842, 1519, 2212, 2224, 2345, 2773
BssMI GATC 7 cut(s) 140, 757, 942, 1828, 2208, 3006, 3115
BssNI GRCGYC 1 cut(s) 2318
BssSI CACGAG 3 cut(s) 435, 446, 2918
BssT1I CCWWGG 7 cut(s) 66, 796, 1519, 2212, 2224, 2345, 2773
Bst2BI CACGAG 3 cut(s) 435, 446, 2918
Bst2UI CCWGG 1 cut(s) 3675
Bst4CI ACNGT 6 cut(s) 605, 638, 2422, 2748, 3424, 3523
Bst6I CTCTTC 4 cut(s) 102, 291, 3441, 3600
BstACI GRCGYC 1 cut(s) 2318
BstAFI CTTAAG 1 cut(s) 3533
BstBAI YACGTR 1 cut(s) 1622
BstBI TTCGAA 1 cut(s) 852
BstDSI CCRYGG 1 cut(s) 2345
BstEII GGTNACC 1 cut(s) 274
BstFNI CGCG 6 cut(s) 624, 779, 971, 1062, 1491, 2406
BstH2I RGCGCY 1 cut(s) 1418
BstHHI GCGC 5 cut(s) 624, 1064, 1417, 1493, 3587
BstKTI GATC 7 cut(s) 143, 760, 945, 1831, 2211, 3009, 3118
BstMBI GATC 7 cut(s) 140, 757, 942, 1828, 2208, 3006, 3115
BstMWI GCNNNNNNNGC 6 cut(s) 759, 869, 897, 1089, 1720, 2950
BstNI CCWGG 1 cut(s) 3675
BstNSI RCATGY 9 cut(s) 95, 232, 265, 1144, 1729, 1903, 2004, 2113, 3295
BstPI GGTNACC 1 cut(s) 274
BstSCI CCNGG 2 cut(s) 2858, 3673
BstSFI CTRYAG 3 cut(s) 643, 3423, 3519
BstSLI GKGCMC 2 cut(s) 2373, 2796
BstSNI TACGTA 1 cut(s) 1622
BstUI CGCG 6 cut(s) 624, 779, 971, 1062, 1491, 2406
BstV1I GCAGC 5 cut(s) 296, 562, 1330, 1918, 3497
BstV2I GAAGAC 6 cut(s) 219, 1875, 2037, 3205, 3567, 3640
BstX2I RGATCY 2 cut(s) 942, 3006
BstXI CCANNNNNNTGG 1 cut(s) 224
BstYI RGATCY 2 cut(s) 942, 3006
Bsu36I CCTNAGG 1 cut(s) 1497
BsuI GTATCC 2 cut(s) 1536, 2425
BsuRI GGCC 5 cut(s) 429, 2344, 2727, 2794, 2858
BtgI CCRYGG 1 cut(s) 2345
BtgZI GCGATG 2 cut(s) 2022, 3384
BtrI CACGTC 1 cut(s) 1132
BtsIMutI CAGTG 4 cut(s) 2445, 3255, 3348, 3420
BveI ACCTGC 1 cut(s) 2043
CciI TCATGA 1 cut(s) 2353
CfoI GCGC 5 cut(s) 624, 1064, 1417, 1493, 3587
Cfr13I GGNCC 6 cut(s) 428, 610, 1586, 2792, 2793, 3313
Csp6I GTAC 9 cut(s) 35, 451, 574, 648, 1394, 2083, 2296, 3375, 3616
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviQI GTAC 9 cut(s) 35, 451, 574, 648, 1394, 2083, 2296, 3375, 3616
DpnI GATC 7 cut(s) 142, 759, 944, 1830, 2210, 3008, 3117
DpnII GATC 7 cut(s) 140, 757, 942, 1828, 2208, 3006, 3115
DrdI GACNNNNNNGTC 1 cut(s) 1701
DseDI GACNNNNNNGTC 1 cut(s) 1701
EaeI YGGCCR 2 cut(s) 2342, 2725
Eam1104I CTCTTC 4 cut(s) 102, 291, 3441, 3600
EarI CTCTTC 4 cut(s) 102, 291, 3441, 3600
EciI GGCGGA 1 cut(s) 1475
Ecl136II GAGCTC 3 cut(s) 416, 1324, 3541
Eco105I TACGTA 1 cut(s) 1622
Eco130I CCWWGG 7 cut(s) 66, 796, 1519, 2212, 2224, 2345, 2773
Eco24I GRGCYC 6 cut(s) 200, 418, 1326, 2796, 3467, 3543
Eco31I GGTCTC 1 cut(s) 1687
Eco32I GATATC 1 cut(s) 394
Eco47I GGWCC 3 cut(s) 610, 1586, 3313
Eco53kI GAGCTC 3 cut(s) 416, 1324, 3541
Eco57I CTGAAG 2 cut(s) 1827, 2469
Eco81I CCTNAGG 1 cut(s) 1497
Eco88I CYCGRG 1 cut(s) 867
Eco91I GGTNACC 1 cut(s) 274
EcoICRI GAGCTC 3 cut(s) 416, 1324, 3541
EcoO109I RGGNCCY 2 cut(s) 428, 2793
EcoO65I GGTNACC 1 cut(s) 274
EcoRI GAATTC 1 cut(s) 3304
EcoRII CCWGG 1 cut(s) 3673
EcoRV GATATC 1 cut(s) 394
EcoT14I CCWWGG 7 cut(s) 66, 796, 1519, 2212, 2224, 2345, 2773
EcoT22I ATGCAT 1 cut(s) 807
EcoT38I GRGCYC 6 cut(s) 200, 418, 1326, 2796, 3467, 3543
ErhI CCWWGG 7 cut(s) 66, 796, 1519, 2212, 2224, 2345, 2773
Esp3I CGTCTC 1 cut(s) 252
FalI AAGNNNNNCTT 8 cut(s) 166, 198, 746, 778, 1140, 1172, 3632, 3664
FaqI GGGAC 5 cut(s) 623, 2510, 2648, 3565, 3582
FauI CCCGC 1 cut(s) 160
Fnu4HI GCNGC 7 cut(s) 285, 551, 1344, 1907, 2342, 2725, 3486
FriOI GRGCYC 6 cut(s) 200, 418, 1326, 2796, 3467, 3543
Fsp4HI GCNGC 7 cut(s) 285, 551, 1344, 1907, 2342, 2725, 3486
FspBI CTAG 6 cut(s) 893, 897, 1121, 1305, 3036, 3378
GlaI GCGC 5 cut(s) 623, 1063, 1416, 1492, 3586
GluI GCNGC 7 cut(s) 285, 551, 1344, 1907, 2342, 2725, 3486
GsuI CTGGAG 1 cut(s) 3477
HaeII RGCGCY 1 cut(s) 1418
HaeIII GGCC 5 cut(s) 429, 2344, 2727, 2794, 2858
HapII CCGG 3 cut(s) 279, 1181, 2859
HhaI GCGC 5 cut(s) 624, 1064, 1417, 1493, 3587
Hin1I GRCGYC 1 cut(s) 2318
Hin6I GCGC 5 cut(s) 622, 1062, 1415, 1491, 3585
HinP1I GCGC 5 cut(s) 622, 1062, 1415, 1491, 3585
HincII GTYRAC 3 cut(s) 961, 3195, 3720
HindII GTYRAC 3 cut(s) 961, 3195, 3720
HindIII AAGCTT 1 cut(s) 3438
HpaII CCGG 3 cut(s) 279, 1181, 2859
Hpy166II GTNNAC 9 cut(s) 451, 576, 961, 1589, 2137, 2445, 2876, 3195, 3720
Hpy8I GTNNAC 9 cut(s) 451, 576, 961, 1589, 2137, 2445, 2876, 3195, 3720
Hpy99I CGWCG 4 cut(s) 1133, 1265, 1560, 3313
HpyCH4III ACNGT 6 cut(s) 605, 638, 2422, 2748, 3424, 3523
HpyCH4IV ACGT 7 cut(s) 1131, 1192, 1621, 1995, 2318, 3075, 3286
HpyF10VI GCNNNNNNNGC 6 cut(s) 759, 869, 897, 1089, 1720, 2950
HpySE526I ACGT 7 cut(s) 1131, 1192, 1621, 1995, 2318, 3075, 3286
Hsp92I GRCGYC 1 cut(s) 2318
HspAI GCGC 5 cut(s) 622, 1062, 1415, 1491, 3585
Kpn2I TCCGGA 1 cut(s) 1180
Kzo9I GATC 7 cut(s) 140, 757, 942, 1828, 2208, 3006, 3115
LguI GCTCTTC 1 cut(s) 291
Lsp1109I GCAGC 5 cut(s) 296, 562, 1330, 1918, 3497
MaeI CTAG 6 cut(s) 893, 897, 1121, 1305, 3036, 3378
MaeII ACGT 7 cut(s) 1131, 1192, 1621, 1995, 2318, 3075, 3286
MaeIII GTNAC 7 cut(s) 274, 735, 1549, 1944, 2074, 3086, 3380
MalI GATC 7 cut(s) 142, 759, 944, 1830, 2210, 3008, 3117
MbiI CCGCTC 1 cut(s) 2724
MboI GATC 7 cut(s) 140, 757, 942, 1828, 2208, 3006, 3115
MfeI CAATTG 3 cut(s) 1351, 1940, 2039
MflI RGATCY 2 cut(s) 942, 3006
MhlI GDGCHC 9 cut(s) 200, 418, 874, 1326, 2373, 2796, 3031, 3467, 3543
MlyI GAGTC 9 cut(s) 112, 602, 923, 998, 2160, 2465, 2609, 2822, 2939
MmeI TCCRAC 5 cut(s) 544, 1077, 1125, 1501, 3276
Mph1103I ATGCAT 1 cut(s) 807
MroI TCCGGA 1 cut(s) 1180
MroXI GAANNNNTTC 2 cut(s) 1152, 2511
MslI CAYNNNNRTG 4 cut(s) 222, 1406, 1730, 2358
MspCI CTTAAG 1 cut(s) 3533
MspI CCGG 3 cut(s) 279, 1181, 2859
MspR9I CCNGG 2 cut(s) 2860, 3675
MunI CAATTG 3 cut(s) 1351, 1940, 2039
MvaI CCWGG 1 cut(s) 3675
MvnI CGCG 6 cut(s) 624, 779, 971, 1062, 1491, 2406
MwoI GCNNNNNNNGC 6 cut(s) 759, 869, 897, 1089, 1720, 2950
NciI CCSGG 1 cut(s) 2860
NcoI CCATGG 1 cut(s) 2345
NdeII GATC 7 cut(s) 140, 757, 942, 1828, 2208, 3006, 3115
NheI GCTAGC 2 cut(s) 896, 3035
NlaIV GGNNCC 5 cut(s) 197, 588, 611, 2794, 3466
NmeAIII GCCGAG 3 cut(s) 1716, 2148, 2479
NmuCI GTSAC 5 cut(s) 274, 735, 2074, 3086, 3380
NsiI ATGCAT 1 cut(s) 807
NspI RCATGY 9 cut(s) 95, 232, 265, 1144, 1729, 1903, 2004, 2113, 3295
NspV TTCGAA 1 cut(s) 852
PaeI GCATGC 2 cut(s) 232, 2113
PaeR7I CTCGAG 1 cut(s) 867
PagI TCATGA 1 cut(s) 2353
PciI ACATGT 2 cut(s) 91, 1899
PciSI GCTCTTC 1 cut(s) 291
PdmI GAANNNNTTC 2 cut(s) 1152, 2511
PkrI GCNGC 7 cut(s) 286, 552, 1345, 1908, 2343, 2726, 3487
PleI GAGTC 9 cut(s) 112, 601, 922, 997, 2160, 2464, 2608, 2822, 2938
PpsI GAGTC 9 cut(s) 112, 601, 922, 997, 2160, 2464, 2608, 2822, 2938
Ppu21I YACGTR 1 cut(s) 1622
PscI ACATGT 2 cut(s) 91, 1899
Psp124BI GAGCTC 3 cut(s) 418, 1326, 3543
Psp6I CCWGG 1 cut(s) 3673
PspEI GGTNACC 1 cut(s) 274
PspGI CCWGG 1 cut(s) 3673
PspN4I GGNNCC 5 cut(s) 197, 588, 611, 2794, 3466
PspOMI GGGCCC 1 cut(s) 2792
PspPI GGNCC 6 cut(s) 428, 610, 1586, 2792, 2793, 3313
PspXI VCTCGAGB 1 cut(s) 867
PsrI GAACNNNNNNTAC 2 cut(s) 2493, 2525
PsuI RGATCY 2 cut(s) 942, 3006
RsaI GTAC 9 cut(s) 36, 452, 575, 649, 1395, 2084, 2297, 3376, 3617
RsaNI GTAC 9 cut(s) 35, 451, 574, 648, 1394, 2083, 2296, 3375, 3616
RseI CAYNNNNRTG 4 cut(s) 222, 1406, 1730, 2358
SacI GAGCTC 3 cut(s) 418, 1326, 3543
SapI GCTCTTC 1 cut(s) 291
SatI GCNGC 7 cut(s) 285, 551, 1344, 1907, 2342, 2725, 3486
Sau3AI GATC 7 cut(s) 140, 757, 942, 1828, 2208, 3006, 3115
Sau96I GGNCC 6 cut(s) 428, 610, 1586, 2792, 2793, 3313
ScaI AGTACT 1 cut(s) 3617
SchI GAGTC 9 cut(s) 112, 602, 923, 998, 2160, 2465, 2609, 2822, 2939
ScrFI CCNGG 2 cut(s) 2860, 3675
SduI GDGCHC 9 cut(s) 200, 418, 874, 1326, 2373, 2796, 3031, 3467, 3543
SfcI CTRYAG 3 cut(s) 643, 3423, 3519
Sfr274I CTCGAG 1 cut(s) 867
SfuI TTCGAA 1 cut(s) 852
SinI GGWCC 3 cut(s) 610, 1586, 3313
SlaI CTCGAG 1 cut(s) 867
SmiMI CAYNNNNRTG 4 cut(s) 222, 1406, 1730, 2358
SmlI CTYRAG 5 cut(s) 867, 1429, 1640, 3030, 3533
SmoI CTYRAG 5 cut(s) 867, 1429, 1640, 3030, 3533
SnaBI TACGTA 1 cut(s) 1622
SpeI ACTAGT 1 cut(s) 3377
SphI GCATGC 2 cut(s) 232, 2113
SspI AATATT 2 cut(s) 1229, 3576
SspMI CTAG 6 cut(s) 893, 897, 1121, 1305, 3036, 3378
SstI GAGCTC 3 cut(s) 418, 1326, 3543
StyD4I CCNGG 2 cut(s) 2858, 3673
StyI CCWWGG 7 cut(s) 66, 796, 1519, 2212, 2224, 2345, 2773
TaaI ACNGT 6 cut(s) 605, 638, 2422, 2748, 3424, 3523
TaiI ACGT 7 cut(s) 1134, 1195, 1624, 1998, 2321, 3078, 3289
TatI WGTACW 3 cut(s) 450, 647, 3615
TauI GCSGC 2 cut(s) 2344, 2727
TscAI CASTG 4 cut(s) 2452, 3255, 3355, 3427
TseFI GTSAC 5 cut(s) 274, 735, 2074, 3086, 3380
TseI GCWGC 5 cut(s) 284, 550, 1343, 1906, 3485
Tsp45I GTSAC 5 cut(s) 274, 735, 2074, 3086, 3380
TspGWI ACGGA 5 cut(s) 1104, 1573, 2039, 2501, 3326
TspRI CASTG 4 cut(s) 2452, 3255, 3355, 3427
Vha464I CTTAAG 1 cut(s) 3533
VpaK11BI GGWCC 3 cut(s) 610, 1586, 3313
XapI RAATTY 4 cut(s) 847, 2396, 3243, 3304
XceI RCATGY 9 cut(s) 95, 232, 265, 1144, 1729, 1903, 2004, 2113, 3295
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XhoI CTCGAG 1 cut(s) 867
XmnI GAANNNNTTC 2 cut(s) 1152, 2511
XspI CTAG 6 cut(s) 893, 897, 1121, 1305, 3036, 3378
ZraI GACGTC 1 cut(s) 2319
ZrmI AGTACT 1 cut(s) 3617
Zsp2I ATGCAT 1 cut(s) 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.