pycom420g00160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0000128
Physical Location & Seq
Reverse (-)
132851 .. 133373
523 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom420g00160.1

Sequence Viewer

Length: 480 bp
ATGCAGTTCGATGGAAAAGGCAACCCGAAGCAACATACCGCCCATTTCATTGAAACTTACAACAATGCTGGAACAGAGGGGGACTACCTCGTCAAACAATTCGTGCGTTCGCTGAAAAGTAATGCTTTTGACTGGTACACCGACCTTGAACCCGAGTCTATCAACAGTTGGGACCAACTAGAAAGGGAATTCCTCAACCGCTTCTACAACACTCGCCGTACTGTAAGCATGCTAGAGCTGACCAGTACGAAACAGTGGAAAGATGAACCTGTCGTCGACTACATCAATAGATGGCACTCCTTAAGTCTAGATTGCAAAGATCGGCTTTCTGAAACCTTTGCCATTGAGATGTACGTTCAAGGCATGCAATGGGGGTTAGATTACATCCTCCAAGGCATAAAACCAAGAACATTCGAAGAGCTAGCAACTCGTTCCCACGACATGGAGCTGAGTATCGCTAATCACGGGAAAAAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.84

Weight (kDa)

5.4

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 32 - 147 1e-05 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 34 - 123 3.7e-14 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 442
AccI GTMKAC 1 cut(s) 276
AciI CCGC 2 cut(s) 39, 199
AcsI RAATTY 1 cut(s) 188
AfaI GTAC 4 cut(s) 137, 220, 247, 353
AfiI CCNNNNNNNGG 1 cut(s) 442
AflII CTTAAG 1 cut(s) 301
AgsI TTSAA 3 cut(s) 53, 149, 359
AluBI AGCT 3 cut(s) 238, 421, 448
AluI AGCT 3 cut(s) 238, 421, 448
Ama87I CYCGRG 1 cut(s) 152
ApoI RAATTY 1 cut(s) 188
AspS9I GGNCC 1 cut(s) 172
AsuII TTCGAA 1 cut(s) 414
AsuNHI GCTAGC 1 cut(s) 421
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 1 cut(s) 172
BccI CCATC 2 cut(s) 5, 285
BceAI ACGGC 1 cut(s) 201
BfaI CTAG 4 cut(s) 179, 233, 308, 422
BfrI CTTAAG 1 cut(s) 301
Bme18I GGWCC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 1 cut(s) 173
BmtI GCTAGC 1 cut(s) 425
Bpu14I TTCGAA 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 391
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse1I ACTGG 2 cut(s) 137, 243
Bse3DI GCAATG 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 391
BseGI GGATG 1 cut(s) 384
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMI GCAATG 1 cut(s) 374
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 2 cut(s) 137, 243
BsiHKCI CYCGRG 1 cut(s) 152
BslFI GGGAC 2 cut(s) 95, 185
BslI CCNNNNNNNGG 1 cut(s) 442
BsmFI GGGAC 2 cut(s) 95, 185
BsoBI CYCGRG 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 414
Bsp143I GATC 1 cut(s) 319
BspACI CCGC 2 cut(s) 39, 199
BspCNI CTCAG 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 173
BspOI GCTAGC 1 cut(s) 425
BspQI GCTCTTC 1 cut(s) 411
BspT104I TTCGAA 1 cut(s) 414
BspTI CTTAAG 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 374
BsrI ACTGG 2 cut(s) 137, 243
BssECI CCNNGG 1 cut(s) 391
BssMI GATC 1 cut(s) 319
BssT1I CCWWGG 1 cut(s) 391
Bst4CI ACNGT 3 cut(s) 167, 223, 255
Bst6I CTCTTC 1 cut(s) 411
BstAFI CTTAAG 1 cut(s) 301
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 230, 365, 423
BstDEI CTNAG 1 cut(s) 449
BstF5I GGATG 1 cut(s) 384
BstKTI GATC 1 cut(s) 322
BstMBI GATC 1 cut(s) 319
BstNSI RCATGY 2 cut(s) 232, 367
BtsCI GGATG 1 cut(s) 384
BtsIMutI CAGTG 1 cut(s) 260
Cac8I GCNNGC 3 cut(s) 230, 365, 423
Cfr13I GGNCC 1 cut(s) 172
Csp6I GTAC 4 cut(s) 136, 219, 246, 352
CviAII CATG 3 cut(s) 229, 364, 442
CviJI RGCY 4 cut(s) 238, 325, 421, 448
CviKI_1 RGCY 4 cut(s) 238, 325, 421, 448
CviQI GTAC 4 cut(s) 136, 219, 246, 352
DdeI CTNAG 1 cut(s) 449
DpnI GATC 1 cut(s) 321
DpnII GATC 1 cut(s) 319
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 172
Eco88I CYCGRG 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 3 cut(s) 232, 367, 445
FaiI YATR 5 cut(s) 36, 230, 365, 398, 443
FalI AAGNNNNNCTT 4 cut(s) 109, 141, 309, 341
FaqI GGGAC 2 cut(s) 95, 185
FatI CATG 3 cut(s) 228, 363, 441
FblI GTMKAC 1 cut(s) 276
FokI GGATG 1 cut(s) 371
FspBI CTAG 4 cut(s) 179, 233, 308, 422
Hin1II CATG 3 cut(s) 232, 367, 445
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 1 cut(s) 155
Hpy166II GTNNAC 2 cut(s) 138, 277
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 2 cut(s) 138, 277
Hpy99I CGWCG 1 cut(s) 278
HpyCH4III ACNGT 3 cut(s) 167, 223, 255
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 3 cut(s) 4, 315, 367
HpyF3I CTNAG 1 cut(s) 449
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 3 cut(s) 232, 367, 445
Kzo9I GATC 1 cut(s) 319
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 1 cut(s) 445
LpnPI CCDG 4 cut(s) 54, 118, 256, 282
MaeI CTAG 4 cut(s) 179, 233, 308, 422
MaeII ACGT 1 cut(s) 354
MalI GATC 1 cut(s) 321
MboI GATC 1 cut(s) 319
MboII GAAGA 1 cut(s) 428
MluCI AATT 2 cut(s) 98, 188
MlyI GAGTC 1 cut(s) 164
MnlI CCTC 4 cut(s) 70, 98, 203, 398
MseI TTAA 1 cut(s) 302
MslI CAYNNNNRTG 1 cut(s) 347
MspCI CTTAAG 1 cut(s) 301
NdeII GATC 1 cut(s) 319
NheI GCTAGC 1 cut(s) 421
NlaIII CATG 3 cut(s) 232, 367, 445
NlaIV GGNNCC 1 cut(s) 173
NspI RCATGY 2 cut(s) 232, 367
NspV TTCGAA 1 cut(s) 414
PaeI GCATGC 2 cut(s) 232, 367
PciSI GCTCTTC 1 cut(s) 411
PflMI CCANNNNNTGG 1 cut(s) 442
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 173
PspPI GGNCC 1 cut(s) 172
RsaI GTAC 4 cut(s) 137, 220, 247, 353
RsaNI GTAC 4 cut(s) 136, 219, 246, 352
RseI CAYNNNNRTG 1 cut(s) 347
SalI GTCGAC 1 cut(s) 275
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 1 cut(s) 302
Sau3AI GATC 1 cut(s) 319
Sau96I GGNCC 1 cut(s) 172
SchI GAGTC 1 cut(s) 164
SetI ASST 8 cut(s) 90, 147, 240, 271, 338, 357, 423, 450
SfuI TTCGAA 1 cut(s) 414
SinI GGWCC 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
SphI GCATGC 2 cut(s) 232, 367
Sse9I AATT 2 cut(s) 98, 188
SsiI CCGC 2 cut(s) 39, 199
SspMI CTAG 4 cut(s) 179, 233, 308, 422
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 3 cut(s) 167, 223, 255
TaiI ACGT 1 cut(s) 357
TaqI TCGA 3 cut(s) 9, 276, 414
TasI AATT 2 cut(s) 98, 188
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TscAI CASTG 1 cut(s) 260
TspDTI ATGAA 2 cut(s) 37, 279
TspRI CASTG 1 cut(s) 260
Van91I CCANNNNNTGG 1 cut(s) 442
Vha464I CTTAAG 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 172
XapI RAATTY 1 cut(s) 188
XbaI TCTAGA 1 cut(s) 307
XceI RCATGY 2 cut(s) 232, 367
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 4 cut(s) 179, 233, 308, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.