Rw1G011350

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000128
Physical Location & Seq
Reverse (-)
25551842 .. 25552761
920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011350.1

Sequence Viewer

Length: 789 bp
ATGCCCTATGGGTACCAGCCGCCCAAGTTTCAGCAATTCGAGGGGAAGGGCAATCCAAAACAACATATTGCTCATTTCGTCGAGACTTGCAACAATGCAGGAACGGAGGGAGATCATCTTGTTAAGCAGTTTGTACGTTCATTAAAAGGCAACGCCTTCGAATGGTACACTGATCTGGAGCCTGAGTCAATCGACAGTTGGGATCAGATGGAGCGTGAGTTCTTGAATCGTTTCTATAGCACGAGACGTACAGTAAGCATGATGGAACTTACTAGCGCCAAGCAATGGAAGGATGAACGCACAATTGATTACATCAACAGGTGGCCTTCGTTGAGTCTCAATTCACACAACATGGAGTTGAGTATAGCTAGCCATAAAGGGAAGAAGGAGTCAATTGTTGACCAAAGAAAAGACAAGGTCTTCGGAAGCAAGTTTGACAAAGCACCGAAGAAATCTACAAAAGAATCGATGGCCGTCAACATTGCGCTCTTCAGAATCTCGACACGAGATAAGGAGGTTAAGAAGGCTGAGCCAACTCGCACCTACGACAGAACAAAGTGTTCGTTAAAGGAATGGCAACAAAAGACATATCCTTTCCCAGATCCTGACGTGGCAGGCATGTTGGAAGATCTACTTGAGAAGAAGGTGATAGAGCTCCCAGAATGCAAGAGGCCCGAGGAAATGCATCGTGTTAAGGATCCGAAATACTGCAAGTACCATCGACTCGTTAGTCACCCAGTGGAGAAATGCTTTGTTCTGAAAGATTTAATAATGAATCTTGCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 Retrotrans_gag

Protein Analysis

262

Amino Acids

30.84

Weight (kDa)

9.07

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 44 - 116 1.9e-09 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 729
Acc65I GGTACC 1 cut(s) 12
AccB1I GGYRCC 1 cut(s) 12
AccB7I CCANNNNNTGG 1 cut(s) 285
AciI CCGC 1 cut(s) 20
AclWI GGATC 4 cut(s) 210, 596, 692, 705
AcoI YGGCCR 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 475
AdeI CACNNNGTG 1 cut(s) 739
AfaI GTAC 5 cut(s) 14, 135, 167, 250, 716
AfiI CCNNNNNNNGG 2 cut(s) 162, 285
AgsI TTSAA 1 cut(s) 226
AjiI CACGTC 1 cut(s) 610
AloI GAACNNNNNNTCC 2 cut(s) 203, 235
AluBI AGCT 2 cut(s) 368, 655
AluI AGCT 2 cut(s) 368, 655
Alw21I GWGCWC 1 cut(s) 657
Alw26I GTCTC 3 cut(s) 77, 238, 341
AlwI GGATC 4 cut(s) 210, 596, 692, 705
AlwNI CAGNNNCTG 1 cut(s) 605
Ama87I CYCGRG 1 cut(s) 674
AoxI GGCC 3 cut(s) 323, 471, 671
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
Asp700I GAANNNNTTC 1 cut(s) 230
Asp718I GGTACC 1 cut(s) 12
AspLEI GCGC 2 cut(s) 278, 487
AspS9I GGNCC 1 cut(s) 672
AsuHPI GGTGA 2 cut(s) 658, 725
AsuII TTCGAA 1 cut(s) 159
AsuNHI GCTAGC 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 674
BamHI GGATCC 1 cut(s) 697
BanI GGYRCC 1 cut(s) 12
BanII GRGCYC 1 cut(s) 657
BauI CACGAG 2 cut(s) 241, 504
BbsI GAAGAC 1 cut(s) 412
Bbv12I GWGCWC 1 cut(s) 657
BccI CCATC 4 cut(s) 202, 256, 463, 726
BceAI ACGGC 1 cut(s) 458
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BcoDI GTCTC 3 cut(s) 77, 238, 341
BfaI CTAG 2 cut(s) 273, 369
BfmI CTRYAG 1 cut(s) 235
BfoI RGCGCY 1 cut(s) 279
BglII AGATCT 1 cut(s) 628
BisI GCNGC 1 cut(s) 20
BlpI GCTNAGC 1 cut(s) 528
BlsI GCNGC 1 cut(s) 21
BmeT110I CYCGRG 1 cut(s) 674
BmgBI CACGTC 1 cut(s) 610
BmgT120I GGNCC 1 cut(s) 672
BmiI GGNNCC 3 cut(s) 14, 180, 699
BmrI ACTGGG 1 cut(s) 731
BmsI GCATC 1 cut(s) 694
BmtI GCTAGC 1 cut(s) 372
BmuI ACTGGG 1 cut(s) 731
BpiI GAAGAC 1 cut(s) 412
BpmI CTGGAG 1 cut(s) 197
Bpu1102I GCTNAGC 1 cut(s) 528
Bpu14I TTCGAA 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 656
Bsa29I ATCGAT 1 cut(s) 467
BsaJI CCNNGG 1 cut(s) 675
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 203, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 285
Bse1I ACTGG 1 cut(s) 737
Bse3DI GCAATG 2 cut(s) 290, 480
BseCI ATCGAT 1 cut(s) 467
BseDI CCNNGG 1 cut(s) 675
BseGI GGATG 1 cut(s) 298
BseLI CCNNNNNNNGG 2 cut(s) 162, 285
BseMI GCAATG 2 cut(s) 290, 480
BseMII CTCAG 2 cut(s) 174, 519
BseNI ACTGG 1 cut(s) 737
BshFI GGCC 3 cut(s) 325, 473, 673
BshNI GGYRCC 1 cut(s) 12
BshVI ATCGAT 1 cut(s) 467
BsiHKAI GWGCWC 1 cut(s) 657
BsiHKCI CYCGRG 1 cut(s) 674
BslI CCNNNNNNNGG 2 cut(s) 162, 285
BsmAI GTCTC 3 cut(s) 77, 238, 341
BsmBI CGTCTC 1 cut(s) 238
BsmI GAATGC 1 cut(s) 668
BsnI GGCC 3 cut(s) 325, 473, 673
BsoBI CYCGRG 1 cut(s) 674
Bsp119I TTCGAA 1 cut(s) 159
Bsp1286I GDGCHC 1 cut(s) 657
Bsp143I GATC 6 cut(s) 112, 172, 202, 601, 628, 697
Bsp1720I GCTNAGC 1 cut(s) 528
BspACI CCGC 1 cut(s) 20
BspANI GGCC 3 cut(s) 325, 473, 673
BspCNI CTCAG 2 cut(s) 175, 520
BspDI ATCGAT 1 cut(s) 467
BspLI GGNNCC 3 cut(s) 14, 180, 699
BspOI GCTAGC 1 cut(s) 372
BspPI GGATC 4 cut(s) 210, 596, 692, 705
BspQI GCTCTTC 1 cut(s) 494
BspT104I TTCGAA 1 cut(s) 159
BspT107I GGYRCC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 290, 480
BsrI ACTGG 1 cut(s) 737
BssECI CCNNGG 1 cut(s) 675
BssMI GATC 6 cut(s) 112, 172, 202, 601, 628, 697
BssSI CACGAG 2 cut(s) 241, 504
Bst2BI CACGAG 2 cut(s) 241, 504
Bst4CI ACNGT 2 cut(s) 197, 253
Bst6I CTCTTC 1 cut(s) 494
BstBI TTCGAA 1 cut(s) 159
BstC8I GCNNGC 2 cut(s) 370, 616
BstDEI CTNAG 2 cut(s) 183, 528
BstF5I GGATG 1 cut(s) 298
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 2 cut(s) 278, 487
BstKTI GATC 6 cut(s) 115, 175, 205, 604, 631, 700
BstMAI GTCTC 3 cut(s) 77, 238, 341
BstMBI GATC 6 cut(s) 112, 172, 202, 601, 628, 697
BstNSI RCATGY 1 cut(s) 622
BstSFI CTRYAG 1 cut(s) 235
BstV2I GAAGAC 1 cut(s) 412
BstX2I RGATCY 3 cut(s) 601, 628, 697
BstYI RGATCY 3 cut(s) 601, 628, 697
Bsu15I ATCGAT 1 cut(s) 467
BsuRI GGCC 3 cut(s) 325, 473, 673
BsuTUI ATCGAT 1 cut(s) 467
BtrI CACGTC 1 cut(s) 610
BtsCI GGATG 1 cut(s) 298
BtsIMutI CAGTG 2 cut(s) 168, 744
Cac8I GCNNGC 2 cut(s) 370, 616
CaiI CAGNNNCTG 1 cut(s) 605
CfoI GCGC 2 cut(s) 278, 487
Cfr13I GGNCC 1 cut(s) 672
ClaI ATCGAT 1 cut(s) 467
Csp6I GTAC 5 cut(s) 13, 134, 166, 249, 715
CviAII CATG 3 cut(s) 259, 352, 619
CviQI GTAC 5 cut(s) 13, 134, 166, 249, 715
DdeI CTNAG 2 cut(s) 183, 528
DpnI GATC 6 cut(s) 114, 174, 204, 603, 630, 699
DpnII GATC 6 cut(s) 112, 172, 202, 601, 628, 697
DraIII CACNNNGTG 1 cut(s) 739
DrdI GACNNNNNNGTC 1 cut(s) 729
DseDI GACNNNNNNGTC 1 cut(s) 729
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
Ecl136II GAGCTC 1 cut(s) 655
Eco24I GRGCYC 1 cut(s) 657
Eco53kI GAGCTC 1 cut(s) 655
Eco57I CTGAAG 1 cut(s) 475
Eco88I CYCGRG 1 cut(s) 674
EcoICRI GAGCTC 1 cut(s) 655
EcoT22I ATGCAT 1 cut(s) 687
EcoT38I GRGCYC 1 cut(s) 657
Esp3I CGTCTC 1 cut(s) 238
FaeI CATG 3 cut(s) 262, 355, 622
FaiI YATR 9 cut(s) 9, 66, 237, 260, 353, 365, 375, 589, 620
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FatI CATG 3 cut(s) 258, 351, 618
Fnu4HI GCNGC 1 cut(s) 20
FokI GGATG 1 cut(s) 305
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 1 cut(s) 20
FspBI CTAG 2 cut(s) 273, 369
GlaI GCGC 2 cut(s) 277, 486
GluI GCNGC 1 cut(s) 20
GsuI CTGGAG 1 cut(s) 197
HaeII RGCGCY 1 cut(s) 279
HaeIII GGCC 3 cut(s) 325, 473, 673
HhaI GCGC 2 cut(s) 278, 487
Hin1II CATG 3 cut(s) 262, 355, 622
Hin6I GCGC 2 cut(s) 276, 485
HinP1I GCGC 2 cut(s) 276, 485
HincII GTYRAC 2 cut(s) 400, 478
HindII GTYRAC 2 cut(s) 400, 478
HinfI GANTC 8 cut(s) 185, 226, 334, 389, 464, 495, 723, 775
HphI GGTGA 2 cut(s) 658, 725
Hpy166II GTNNAC 3 cut(s) 168, 400, 478
Hpy188I TCNGA 5 cut(s) 207, 425, 494, 702, 759
Hpy188III TCNNGA 5 cut(s) 82, 176, 223, 499, 605
Hpy8I GTNNAC 3 cut(s) 168, 400, 478
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 7 cut(s) 40, 166, 283, 336, 379, 517, 637
HpyCH4III ACNGT 2 cut(s) 197, 253
HpyCH4IV ACGT 3 cut(s) 136, 247, 609
HpyCH4V TGCA 5 cut(s) 90, 98, 666, 685, 711
HpyF3I CTNAG 2 cut(s) 183, 528
HpySE526I ACGT 3 cut(s) 136, 247, 609
Hsp92II CATG 3 cut(s) 262, 355, 622
HspAI GCGC 2 cut(s) 276, 485
KpnI GGTACC 1 cut(s) 16
Kzo9I GATC 6 cut(s) 112, 172, 202, 601, 628, 697
LguI GCTCTTC 1 cut(s) 494
LmnI GCTCC 3 cut(s) 178, 211, 660
LweI GCATC 1 cut(s) 694
MaeI CTAG 2 cut(s) 273, 369
MaeII ACGT 3 cut(s) 136, 247, 609
MaeIII GTNAC 1 cut(s) 731
MalI GATC 6 cut(s) 114, 174, 204, 603, 630, 699
MboI GATC 6 cut(s) 112, 172, 202, 601, 628, 697
MboII GAAGA 6 cut(s) 394, 412, 460, 481, 638, 652
MfeI CAATTG 2 cut(s) 303, 393
MflI RGATCY 3 cut(s) 601, 628, 697
MhlI GDGCHC 1 cut(s) 657
MluCI AATT 4 cut(s) 35, 303, 340, 393
MlyI GAGTC 4 cut(s) 194, 343, 398, 717
MmeI TCCRAC 1 cut(s) 603
MnlI CCTC 5 cut(s) 34, 100, 508, 663, 670
Mph1103I ATGCAT 1 cut(s) 687
MroXI GAANNNNTTC 1 cut(s) 230
MseI TTAA 6 cut(s) 123, 143, 519, 566, 693, 767
MunI CAATTG 2 cut(s) 303, 393
Mva1269I GAATGC 1 cut(s) 668
NdeII GATC 6 cut(s) 112, 172, 202, 601, 628, 697
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 3 cut(s) 262, 355, 622
NlaIV GGNNCC 3 cut(s) 14, 180, 699
NmuCI GTSAC 1 cut(s) 731
NsiI ATGCAT 1 cut(s) 687
NspI RCATGY 1 cut(s) 622
NspV TTCGAA 1 cut(s) 159
PciSI GCTCTTC 1 cut(s) 494
PctI GAATGC 1 cut(s) 668
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 4 cut(s) 226, 464, 495, 775
PflFI GACNNNGTC 1 cut(s) 416
PflMI CCANNNNNTGG 1 cut(s) 285
PkrI GCNGC 1 cut(s) 21
PleI GAGTC 4 cut(s) 193, 342, 397, 717
PpsI GAGTC 4 cut(s) 193, 342, 397, 717
Psp124BI GAGCTC 1 cut(s) 657
PspN4I GGNNCC 3 cut(s) 14, 180, 699
PspPI GGNCC 1 cut(s) 672
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 3 cut(s) 601, 628, 697
PsyI GACNNNGTC 1 cut(s) 416
RsaI GTAC 5 cut(s) 14, 135, 167, 250, 716
RsaNI GTAC 5 cut(s) 13, 134, 166, 249, 715
SacI GAGCTC 1 cut(s) 657
SapI GCTCTTC 1 cut(s) 494
SaqAI TTAA 6 cut(s) 123, 143, 519, 566, 693, 767
SatI GCNGC 1 cut(s) 20
Sau3AI GATC 6 cut(s) 112, 172, 202, 601, 628, 697
Sau96I GGNCC 1 cut(s) 672
SchI GAGTC 4 cut(s) 194, 343, 398, 717
SduI GDGCHC 1 cut(s) 657
SfaNI GCATC 1 cut(s) 694
SfcI CTRYAG 1 cut(s) 235
SfuI TTCGAA 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
Sse9I AATT 4 cut(s) 35, 303, 340, 393
SsiI CCGC 1 cut(s) 20
SspMI CTAG 2 cut(s) 273, 369
SstI GAGCTC 1 cut(s) 657
TaaI ACNGT 2 cut(s) 197, 253
TaiI ACGT 3 cut(s) 139, 250, 612
TaqI TCGA 7 cut(s) 39, 81, 159, 192, 467, 500, 721
TasI AATT 4 cut(s) 35, 303, 340, 393
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 4 cut(s) 226, 464, 495, 775
Tru1I TTAA 6 cut(s) 123, 143, 519, 566, 693, 767
Tru9I TTAA 6 cut(s) 123, 143, 519, 566, 693, 767
TscAI CASTG 2 cut(s) 175, 744
TseFI GTSAC 1 cut(s) 731
Tsp45I GTSAC 1 cut(s) 731
TspDTI ATGAA 3 cut(s) 129, 309, 788
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 2 cut(s) 175, 744
Tth111I GACNNNGTC 1 cut(s) 416
Van91I CCANNNNNTGG 1 cut(s) 285
XceI RCATGY 1 cut(s) 622
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 2 cut(s) 273, 369
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.