pycom09g14890

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000128
Physical Location & Seq
Forward (+)
14693568 .. 14696264
2697 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14890.1

Sequence Viewer

Length: 2655 bp
ATGGCTTCAAACAAAGAACAAGTCATGACTATCGGCACTACCTCCATTGAAAAACAGCTGGTTCAGATGAAGGAAACAATTGCAAGGCTGATAAAGACTGCAGAGGAGAAAAACTTGCAAACCGCCGGACTCATCAACCGTCTGGAGGCGCAACATGGTGTCATAGTGAAGCCAAACTCTCTTCCAACTAAGAGGACCGAAGAAGGCTTCAACCCAGATGCCTACGAACTCATGTCAAAGGCTGGGAATGACTTTGCTTCCTCTTCAAATCTTGGAAAGAAGGTTTCAAACACCGTCAACGACAAAGATCGCGACCTCACCGAGACTCAAAAGAAGTTGAAGGAGCATGATTATGGAGTTGACAACAACAAGGCTGGACTTGGCTTCACACCAAATGCACCCGTGAAGATTTCAAGCAAAGTGAAAAAAGCTAGCGCTCAACACATCAGCGTGAGCATTGAACAAGACCAAGAAGAGCCTAAACCCTCCCCTCGGATGTCGGTCTTCAATAGGATGAGCCATTCAAGCTCCAGAACTTCAGTGCTTAATCGCATTGGTGGTCAAGACCGAACCTCTGTCTTCAAGAGGCTTAACGCGCCGACATCCCAAAGCTCTGTTTTTGAAAGGTTGTTAAAGCCTAAGAAACAAAGCAACACAGCTATCTCTCCTCTGCAACGATCAGCTTCAGAAAGATTTGGAAAGAGGAAGACAACACCAAAGGAAGAAAAGCTCGATGGGTTAGGAGAAAAAGACGACGCTCGAAGCTTGATTCCTTCAAGGATGGAGCGCCAAGCAATCCTAGAGGTCGACACAAAAGGACCACTGAAGGTAAGGAGGCGCATCATTGTCCACACTGGCAAATCTTCATGCCAACAAACCCAAGAGAACGGCACTGAAGAAGAGATCCAAGATGTTTTCTACATCACGGTTCAAGAAGGTAAAGAAGACGAAAGCCCGAGGAAATACTCAGAGTCCCTACTCTCCGACCCTGATCCAAGCGGCAGTACAACCATGCAAGTCATGACCACTGGAGCAACTTCAATCGAGGAGCAGCTAGCTCGGATGAATGAAGCAATCGCAAAGCTCACACGGACTGTGGAAGAGAAGGACTTGCAGATTGCCGCACTTGTCAACCAACTAGATGCGCTGCCCGACGTAAAAGTCGACCCAAATATTGGCCTATTAAAGAAAGAAGGCGACGAAGAAGAGGAGCCACCAGTGGAGAAAGTGGAAGAGAAGCCGAAGCTAGACCAAGCAACGGCATTCATGGGATCTCTCTCTATCCAGCAGCTACAGGAGATGATCGCAAGCACTGTCAAGACACAGTACGAAGGAAGCTCGCATGACTCCGTACTGTACTCAAAGCCTTACTCCAAGAAGATTGATGCTTTGAAGATGCCAAGGGGTTATCAGCCCCCAAAATTCATGCAGTTCGATGGAAAAGGCAACCCGAAGCAACATGTCGCCCATTTCGTTGAAACTTGCAACAATGCTGGGACAGAGGGAGACTACCTCGTCAAACAGTTCGTGCGTTCGCTGAAAAGTAACGCTTTTGACTGGTACACTGACCTCGAACCTGAGTCTATCAACAGTTGGGACCAGCTAGAAAGGGAATTCCTCAACCGTTTCTACAACACTCGTCGTACTGAACCTGTCATCGACTACATAAATAGATGGCGTTCTTTAAGTCTGGATTGCAAAGATCGGCTTTCTGAAACCTCTGCCATTGAGATGTGCGTTCAAGGCATGCAATGGGGATTACACTACATCCTTCAAGGCATCAAACCACGAACATTTGAGGAGTTAGCCACCCGCGCCCATGATATGGAGTTAAGCATCACCCGTCATGGGAAGAAAGAACAGATCGCCGACTACAAGAATGACAAAGTTTTGGGGCCCAAGGTGGATAAGGCTGCGTGGAAACCCACCAAGGAAGCAATGACGCTCAACACAACTCCAGTCAAAACCCATACACGAGGCAAGGCGATTCAAACCGAAGCTTTTCGTGATCAAGAGATACGTATACGCACTTTGAAGGAGCTTGAGGAGAAGACTTATCCATTCCCCGACTCTGACGTGGCTGCCATGTTAGATGACTTGCTGAAAAAAAAGGTGATCGACCTACCTGAGTGTAAACGGCCAGAAGAGATGAATCGTACTGACAGTCCAAGGTACTGTAAATTCCACCGCTTCATTGGTCATCCGACGGAAAAGTGCTTTGTGCTAAAAGATCTCATCATGAAGTTGGCTCAGAAAGGGATCATCGAGCTAGATCTTAATGATGTGGTGAAGTCAAACTATATCACCGCCACTTCTGGCTCTTTGAACTCAAAATCTTCACCTCAACCGCTAGGGGCATGCTCCAAAACCATGCCAGTCAAGTCAAGTGAAGTTAAAGGATGGACTTATGTCACTCCAAAGAAAATGCACAAGAAACATAGGTCTCCTCCACAAGTTCACCAATCGGAAAGGGGGCAAAGCAGCTACCGTCAACCTTCAAAGCTACATGAAAAGGTTGAGGATGATGAAGCTATGACACAAAGATCGTCCGTTGCCATCACGATGCGCGATTTCTTCCCCAAAGACTTCTTCAATCACTCAGTCAAGACTCCTTGCTATGAAGATTGCAAGGAATGCCTCTCTAAGATCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

885

Amino Acids

100.14

Weight (kDa)

8.76

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 509 - 584 3.3e-07 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1160, 1514
AccB7I CCANNNNNTGG 2 cut(s) 1175, 1825
AccI GTMKAC 3 cut(s) 807, 1164, 2023
AccII CGCG 4 cut(s) 312, 596, 1815, 2568
AciI CCGC 7 cut(s) 123, 999, 1122, 1813, 2188, 2307, 2348
AclWI GGATC 4 cut(s) 898, 986, 1279, 2267
AcoI YGGCCR 1 cut(s) 2138
AcsI RAATTY 3 cut(s) 1421, 1613, 2180
AcuI CTGAAG 4 cut(s) 522, 669, 845, 915
AfaI GTAC 8 cut(s) 1006, 1328, 1353, 1358, 1562, 1645, 2158, 2174
AfeI AGCGCT 1 cut(s) 436
AfiI CCNNNNNNNGG 6 cut(s) 145, 492, 1175, 1258, 1825, 1848
AflIII ACRYGT 1 cut(s) 1459
AjiI CACGTC 1 cut(s) 2077
AjuI GAANNNNNNNTTGG 2 cut(s) 2573, 2605
Alw26I GTCTC 3 cut(s) 317, 1500, 2448
AlwI GGATC 4 cut(s) 898, 986, 1279, 2267
Ama87I CYCGRG 1 cut(s) 955
Aor51HI AGCGCT 1 cut(s) 436
AoxI GGCC 3 cut(s) 1177, 1895, 2138
ApaI GGGCCC 1 cut(s) 1899
ApeKI GCWGC 6 cut(s) 1051, 1147, 1288, 1913, 2081, 2481
ApoI RAATTY 3 cut(s) 1421, 1613, 2180
ArsI GACNNNNNNTTYG 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 2001
AspLEI GCGC 8 cut(s) 151, 437, 598, 789, 840, 1147, 1817, 2568
AspS9I GGNCC 5 cut(s) 195, 818, 1597, 1895, 1896
AsuHPI GGTGA 7 cut(s) 310, 1831, 2125, 2296, 2299, 2331, 2450
AsuNHI GCTAGC 2 cut(s) 431, 1054
AvaI CYCGRG 1 cut(s) 955
AvaII GGWCC 3 cut(s) 195, 818, 1597
BaeGI GKGCMC 1 cut(s) 1899
BanII GRGCYC 1 cut(s) 1899
BauI CACGAG 1 cut(s) 1974
BbsI GAAGAC 5 cut(s) 496, 571, 713, 951, 2057
BbvI GCAGC 6 cut(s) 1063, 1134, 1300, 1900, 2068, 2493
BccI CCATC 6 cut(s) 728, 775, 1430, 1668, 2394, 2564
BceAI ACGGC 3 cut(s) 904, 1275, 2153
BcgI CGANNNNNNTGC 2 cut(s) 1040, 1074
BclI TGATCA 1 cut(s) 2008
BcoDI GTCTC 3 cut(s) 317, 1500, 2448
BfaI CTAG 8 cut(s) 432, 800, 1055, 1139, 1247, 1604, 2270, 2351
BfmI CTRYAG 2 cut(s) 99, 1292
BfoI RGCGCY 2 cut(s) 438, 790
BglII AGATCT 2 cut(s) 2230, 2272
BisI GCNGC 8 cut(s) 1000, 1052, 1122, 1148, 1289, 1914, 2082, 2482
BlsI GCNGC 8 cut(s) 1001, 1053, 1123, 1149, 1290, 1915, 2083, 2483
Bme18I GGWCC 3 cut(s) 195, 818, 1597
BmeT110I CYCGRG 1 cut(s) 955
BmgBI CACGTC 1 cut(s) 2077
BmgT120I GGNCC 5 cut(s) 195, 818, 1597, 1895, 1896
BmiI GGNNCC 4 cut(s) 1212, 1598, 1896, 1897
BmsI GCATC 8 cut(s) 208, 849, 1132, 1375, 1386, 1788, 1845, 2553
BmtI GCTAGC 2 cut(s) 435, 1058
BoxI GACNNNNGTC 1 cut(s) 2408
BpiI GAAGAC 5 cut(s) 496, 571, 713, 951, 2057
BplI GAGNNNNNCTC 2 cut(s) 1497, 1529
BpmI CTGGAG 4 cut(s) 164, 514, 1050, 1941
BpuEI CTTGAG 1 cut(s) 2063
BsaAI YACGTR 1 cut(s) 2021
BsaI GGTCTC 1 cut(s) 2448
BsaJI CCNNGG 6 cut(s) 491, 956, 1400, 1899, 1929, 2168
Bsc4I CCNNNNNNNGG 6 cut(s) 145, 492, 1175, 1258, 1825, 1848
Bse1I ACTGG 6 cut(s) 859, 1033, 1217, 1562, 1958, 2375
Bse3DI GCAATG 2 cut(s) 1757, 1944
BseDI CCNNGG 6 cut(s) 491, 956, 1400, 1899, 1929, 2168
BseGI GGATG 9 cut(s) 501, 519, 602, 786, 1068, 1767, 2200, 2405, 2527
BseLI CCNNNNNNNGG 6 cut(s) 145, 492, 1175, 1258, 1825, 1848
BseMI GCAATG 2 cut(s) 1757, 1944
BseMII CTCAG 5 cut(s) 981, 1569, 2118, 2264, 2613
BseNI ACTGG 6 cut(s) 859, 1033, 1217, 1562, 1958, 2375
BseRI GAGGAG 7 cut(s) 119, 657, 1061, 1223, 1814, 2060, 2436
BseSI GKGCMC 1 cut(s) 1899
BseXI GCAGC 6 cut(s) 1063, 1134, 1300, 1900, 2068, 2493
BseYI CCCAGC 2 cut(s) 242, 1493
Bsh1236I CGCG 4 cut(s) 312, 596, 1815, 2568
BshFI GGCC 3 cut(s) 1179, 1897, 2140
BsiHKCI CYCGRG 1 cut(s) 955
BsiSI CCGG 1 cut(s) 126
BslFI GGGAC 3 cut(s) 958, 1510, 1610
BslI CCNNNNNNNGG 6 cut(s) 145, 492, 1175, 1258, 1825, 1848
BsmAI GTCTC 3 cut(s) 317, 1500, 2448
BsmFI GGGAC 3 cut(s) 958, 1510, 1610
BsmI GAATGC 2 cut(s) 1262, 2639
BsnI GGCC 3 cut(s) 1179, 1897, 2140
Bso31I GGTCTC 1 cut(s) 2448
BsoBI CYCGRG 1 cut(s) 955
Bsp120I GGGCCC 1 cut(s) 1895
Bsp1286I GDGCHC 1 cut(s) 1899
Bsp68I TCGCGA 1 cut(s) 312
BspACI CCGC 7 cut(s) 123, 999, 1122, 1813, 2188, 2307, 2348
BspANI GGCC 3 cut(s) 1179, 1897, 2140
BspCNI CTCAG 5 cut(s) 980, 1570, 2119, 2263, 2612
BspFNI CGCG 4 cut(s) 312, 596, 1815, 2568
BspHI TCATGA 3 cut(s) 24, 1020, 2238
BspLI GGNNCC 4 cut(s) 1212, 1598, 1896, 1897
BspMAI CTGCAG 1 cut(s) 103
BspOI GCTAGC 2 cut(s) 435, 1058
BspPI GGATC 4 cut(s) 898, 986, 1279, 2267
BspQI GCTCTTC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 2448
BsrDI GCAATG 2 cut(s) 1757, 1944
BsrI ACTGG 6 cut(s) 859, 1033, 1217, 1562, 1958, 2375
BssECI CCNNGG 6 cut(s) 491, 956, 1400, 1899, 1929, 2168
BssNAI GTATAC 1 cut(s) 2024
BssSI CACGAG 1 cut(s) 1974
BssT1I CCWWGG 4 cut(s) 1400, 1899, 1929, 2168
Bst1107I GTATAC 1 cut(s) 2024
Bst2BI CACGAG 1 cut(s) 1974
Bst6I CTCTTC 8 cut(s) 186, 268, 468, 894, 1095, 1200, 1227, 2139
BstAPI GCANNNNNTGC 1 cut(s) 2634
BstBAI YACGTR 1 cut(s) 2021
BstC8I GCNNGC 6 cut(s) 433, 1056, 1309, 1340, 1748, 2359
BstDEI CTNAG 8 cut(s) 189, 639, 967, 1578, 2127, 2250, 2599, 2643
BstF5I GGATG 9 cut(s) 501, 519, 602, 786, 1068, 1767, 2200, 2405, 2527
BstFNI CGCG 4 cut(s) 312, 596, 1815, 2568
BstH2I RGCGCY 2 cut(s) 438, 790
BstHHI GCGC 8 cut(s) 151, 437, 598, 789, 840, 1147, 1817, 2568
BstMAI GTCTC 3 cut(s) 317, 1500, 2448
BstMWI GCNNNNNNNGC 5 cut(s) 525, 595, 1743, 1814, 2634
BstNSI RCATGY 3 cut(s) 1463, 1750, 2361
BstPAI GACNNNNGTC 1 cut(s) 2408
BstSFI CTRYAG 2 cut(s) 99, 1292
BstSLI GKGCMC 1 cut(s) 1899
BstSNI TACGTA 1 cut(s) 2021
BstUI CGCG 4 cut(s) 312, 596, 1815, 2568
BstV1I GCAGC 6 cut(s) 1063, 1134, 1300, 1900, 2068, 2493
BstV2I GAAGAC 5 cut(s) 496, 571, 713, 951, 2057
BstX2I RGATCY 4 cut(s) 903, 1271, 2230, 2272
BstYI RGATCY 4 cut(s) 903, 1271, 2230, 2272
BstZ17I GTATAC 1 cut(s) 2024
BsuRI GGCC 3 cut(s) 1179, 1897, 2140
BtrI CACGTC 1 cut(s) 2077
BtsCI GGATG 9 cut(s) 501, 519, 602, 786, 1068, 1767, 2200, 2405, 2527
BtsIMutI CAGTG 8 cut(s) 546, 821, 852, 891, 1026, 1224, 1311, 1563
BtuMI TCGCGA 1 cut(s) 312
Cac8I GCNNGC 6 cut(s) 433, 1056, 1309, 1340, 1748, 2359
CciI TCATGA 3 cut(s) 24, 1020, 2238
CfoI GCGC 8 cut(s) 151, 437, 598, 789, 840, 1147, 1817, 2568
Cfr13I GGNCC 5 cut(s) 195, 818, 1597, 1895, 1896
CseI GACGC 2 cut(s) 764, 1951
Csp6I GTAC 8 cut(s) 1005, 1327, 1352, 1357, 1561, 1644, 2157, 2173
CviQI GTAC 8 cut(s) 1005, 1327, 1352, 1357, 1561, 1644, 2157, 2173
DdeI CTNAG 8 cut(s) 189, 639, 967, 1578, 2127, 2250, 2599, 2643
DrdI GACNNNNNNGTC 2 cut(s) 1160, 1514
DseDI GACNNNNNNGTC 2 cut(s) 1160, 1514
EaeI YGGCCR 1 cut(s) 2138
Eam1104I CTCTTC 8 cut(s) 186, 268, 468, 894, 1095, 1200, 1227, 2139
EarI CTCTTC 8 cut(s) 186, 268, 468, 894, 1095, 1200, 1227, 2139
Eco105I TACGTA 1 cut(s) 2021
Eco130I CCWWGG 4 cut(s) 1400, 1899, 1929, 2168
Eco24I GRGCYC 1 cut(s) 1899
Eco31I GGTCTC 1 cut(s) 2448
Eco47I GGWCC 3 cut(s) 195, 818, 1597
Eco47III AGCGCT 1 cut(s) 436
Eco57I CTGAAG 4 cut(s) 522, 669, 845, 915
Eco88I CYCGRG 1 cut(s) 955
EcoO109I RGGNCCY 1 cut(s) 1895
EcoRI GAATTC 1 cut(s) 1613
EcoT14I CCWWGG 4 cut(s) 1400, 1899, 1929, 2168
EcoT38I GRGCYC 1 cut(s) 1899
ErhI CCWWGG 4 cut(s) 1400, 1899, 1929, 2168
FalI AAGNNNNNCTT 4 cut(s) 1534, 1566, 1692, 1724
FaqI GGGAC 3 cut(s) 958, 1510, 1610
FauI CCCGC 1 cut(s) 1820
FbaI TGATCA 1 cut(s) 2008
FblI GTMKAC 3 cut(s) 807, 1164, 2023
Fnu4HI GCNGC 8 cut(s) 1000, 1052, 1122, 1148, 1289, 1914, 2082, 2482
FokI GGATG 9 cut(s) 508, 526, 589, 793, 1075, 1754, 2187, 2412, 2534
FriOI GRGCYC 1 cut(s) 1899
Fsp4HI GCNGC 8 cut(s) 1000, 1052, 1122, 1148, 1289, 1914, 2082, 2482
FspBI CTAG 8 cut(s) 432, 800, 1055, 1139, 1247, 1604, 2270, 2351
GlaI GCGC 8 cut(s) 150, 436, 597, 788, 839, 1146, 1816, 2567
GluI GCNGC 8 cut(s) 1000, 1052, 1122, 1148, 1289, 1914, 2082, 2482
GsaI CCCAGC 2 cut(s) 246, 1497
GsuI CTGGAG 4 cut(s) 164, 514, 1050, 1941
HaeII RGCGCY 2 cut(s) 438, 790
HaeIII GGCC 3 cut(s) 1179, 1897, 2140
HapII CCGG 1 cut(s) 126
HgaI GACGC 2 cut(s) 764, 1951
HhaI GCGC 8 cut(s) 151, 437, 598, 789, 840, 1147, 1817, 2568
Hin6I GCGC 8 cut(s) 149, 435, 596, 787, 838, 1145, 1815, 2566
HinP1I GCGC 8 cut(s) 149, 435, 596, 787, 838, 1145, 1815, 2566
HincII GTYRAC 6 cut(s) 298, 361, 808, 1132, 1165, 2492
HindII GTYRAC 6 cut(s) 298, 361, 808, 1132, 1165, 2492
HindIII AAGCTT 2 cut(s) 763, 1998
HpaII CCGG 1 cut(s) 126
HphI GGTGA 7 cut(s) 310, 1831, 2125, 2296, 2299, 2331, 2450
Hpy99I CGWCG 5 cut(s) 758, 1157, 1202, 1644, 2209
HpyCH4IV ACGT 3 cut(s) 1155, 2020, 2076
HpyF10VI GCNNNNNNNGC 5 cut(s) 525, 595, 1743, 1814, 2634
HpyF3I CTNAG 8 cut(s) 189, 639, 967, 1578, 2127, 2250, 2599, 2643
HpySE526I ACGT 3 cut(s) 1155, 2020, 2076
HspAI GCGC 8 cut(s) 149, 435, 596, 787, 838, 1145, 1815, 2566
Ksp22I TGATCA 1 cut(s) 2008
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 8 cut(s) 343, 533, 784, 1031, 1048, 1210, 2038, 2366
Lsp1109I GCAGC 6 cut(s) 1063, 1134, 1300, 1900, 2068, 2493
LweI GCATC 8 cut(s) 208, 849, 1132, 1375, 1386, 1788, 1845, 2553
MaeI CTAG 8 cut(s) 432, 800, 1055, 1139, 1247, 1604, 2270, 2351
MaeII ACGT 3 cut(s) 1155, 2020, 2076
MaeIII GTNAC 2 cut(s) 1544, 2410
MfeI CAATTG 1 cut(s) 78
MflI RGATCY 4 cut(s) 903, 1271, 2230, 2272
MhlI GDGCHC 1 cut(s) 1899
MluCI AATT 4 cut(s) 78, 1421, 1613, 2180
MlyI GAGTC 7 cut(s) 123, 319, 980, 1340, 1589, 2063, 2602
MmeI TCCRAC 3 cut(s) 209, 1008, 2228
MroXI GAANNNNTTC 1 cut(s) 2001
MseI TTAA 8 cut(s) 546, 591, 632, 1184, 1685, 1832, 2277, 2394
MslI CAYNNNNRTG 4 cut(s) 351, 449, 1730, 2561
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 1 cut(s) 126
MunI CAATTG 1 cut(s) 78
Mva1269I GAATGC 2 cut(s) 1262, 2639
MvnI CGCG 4 cut(s) 312, 596, 1815, 2568
MwoI GCNNNNNNNGC 5 cut(s) 525, 595, 1743, 1814, 2634
NheI GCTAGC 2 cut(s) 431, 1054
NlaIV GGNNCC 4 cut(s) 1212, 1598, 1896, 1897
NmuCI GTSAC 1 cut(s) 2410
NruI TCGCGA 1 cut(s) 312
NspI RCATGY 3 cut(s) 1463, 1750, 2361
PaeI GCATGC 2 cut(s) 1750, 2361
PagI TCATGA 3 cut(s) 24, 1020, 2238
PciI ACATGT 1 cut(s) 1459
PciSI GCTCTTC 1 cut(s) 468
PcsI WCGNNNNNNNCGW 2 cut(s) 1161, 1470
PctI GAATGC 2 cut(s) 1262, 2639
PdmI GAANNNNTTC 1 cut(s) 2001
PfeI GAWTC 3 cut(s) 769, 1987, 2152
PflMI CCANNNNNTGG 2 cut(s) 1175, 1825
PkrI GCNGC 8 cut(s) 1001, 1053, 1123, 1149, 1290, 1915, 2083, 2483
PleI GAGTC 7 cut(s) 123, 319, 979, 1340, 1588, 2063, 2602
PpsI GAGTC 7 cut(s) 123, 319, 979, 1340, 1588, 2063, 2602
Ppu21I YACGTR 1 cut(s) 2021
PscI ACATGT 1 cut(s) 1459
PshAI GACNNNNGTC 1 cut(s) 2408
PspFI CCCAGC 2 cut(s) 242, 1493
PspN4I GGNNCC 4 cut(s) 1212, 1598, 1896, 1897
PspOMI GGGCCC 1 cut(s) 1895
PspPI GGNCC 5 cut(s) 195, 818, 1597, 1895, 1896
PstI CTGCAG 1 cut(s) 103
PsuI RGATCY 4 cut(s) 903, 1271, 2230, 2272
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 312
RsaI GTAC 8 cut(s) 1006, 1328, 1353, 1358, 1562, 1645, 2158, 2174
RsaNI GTAC 8 cut(s) 1005, 1327, 1352, 1357, 1561, 1644, 2157, 2173
RseI CAYNNNNRTG 4 cut(s) 351, 449, 1730, 2561
SalI GTCGAC 2 cut(s) 806, 1163
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 8 cut(s) 546, 591, 632, 1184, 1685, 1832, 2277, 2394
SatI GCNGC 8 cut(s) 1000, 1052, 1122, 1148, 1289, 1914, 2082, 2482
Sau96I GGNCC 5 cut(s) 195, 818, 1597, 1895, 1896
SchI GAGTC 7 cut(s) 123, 319, 980, 1340, 1589, 2063, 2602
SduI GDGCHC 1 cut(s) 1899
SfaNI GCATC 8 cut(s) 208, 849, 1132, 1375, 1386, 1788, 1845, 2553
SfcI CTRYAG 2 cut(s) 99, 1292
SinI GGWCC 3 cut(s) 195, 818, 1597
SmiMI CAYNNNNRTG 4 cut(s) 351, 449, 1730, 2561
SmlI CTYRAG 1 cut(s) 2042
SmoI CTYRAG 1 cut(s) 2042
SnaBI TACGTA 1 cut(s) 2021
SphI GCATGC 2 cut(s) 1750, 2361
Sse9I AATT 4 cut(s) 78, 1421, 1613, 2180
SsiI CCGC 7 cut(s) 123, 999, 1122, 1813, 2188, 2307, 2348
SspI AATATT 1 cut(s) 1174
SspMI CTAG 8 cut(s) 432, 800, 1055, 1139, 1247, 1604, 2270, 2351
StyI CCWWGG 4 cut(s) 1400, 1899, 1929, 2168
TaiI ACGT 3 cut(s) 1158, 2023, 2079
TaqII GACCGA 3 cut(s) 212, 490, 582
TasI AATT 4 cut(s) 78, 1421, 1613, 2180
TatI WGTACW 2 cut(s) 1004, 1356
TauI GCSGC 2 cut(s) 1002, 1124
TfiI GAWTC 3 cut(s) 769, 1987, 2152
Tru1I TTAA 8 cut(s) 546, 591, 632, 1184, 1685, 1832, 2277, 2394
Tru9I TTAA 8 cut(s) 546, 591, 632, 1184, 1685, 1832, 2277, 2394
TscAI CASTG 8 cut(s) 546, 828, 859, 898, 1033, 1224, 1318, 1570
TseFI GTSAC 1 cut(s) 2410
TseI GCWGC 6 cut(s) 1051, 1147, 1288, 1913, 2081, 2481
Tsp45I GTSAC 1 cut(s) 2410
TspGWI ACGGA 4 cut(s) 1105, 1339, 2222, 2539
TspRI CASTG 8 cut(s) 546, 828, 859, 898, 1033, 1224, 1318, 1570
Van91I CCANNNNNTGG 2 cut(s) 1175, 1825
VpaK11BI GGWCC 3 cut(s) 195, 818, 1597
XapI RAATTY 3 cut(s) 1421, 1613, 2180
XceI RCATGY 3 cut(s) 1463, 1750, 2361
XcmI CCANNNNNNNNNTGG 1 cut(s) 2192
XmiI GTMKAC 3 cut(s) 807, 1164, 2023
XmnI GAANNNNTTC 1 cut(s) 2001
XspI CTAG 8 cut(s) 432, 800, 1055, 1139, 1247, 1604, 2270, 2351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.