pycom06g05750

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000128
Physical Location & Seq
Reverse (-)
8674231 .. 8685013
10783 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g05750.1

Sequence Viewer

Length: 2004 bp
ATGGCTTCAAACAAAGAACAAGTCATGACTATCGGCACTACCTCCATTGAAAAACAGCTGGTTCAGATGAAGGAAACAATTGCAAGGCTGATAAAGACTGCAGAGGAGAAAAACTTGCAAACCGCCGGACTCATCAACCGTCTGGAGGCGCAACATGGTGTCATAGTGAAACCAAGCTCTCTTCCAACTAAGAGGACCGAAGAAGGCTTTAACCCAAATGCCTACGAACTCATGTCAAAGGCTAGGCATGACTTTGCTTCCCCTTCAAATCTTGGAAAGAAGGTTTCAAACACCGTCAACGACAAAGATCGCGACCTCACCGAGACTCAAAAGAAGTTGAAGGAGCATGATTATGGAGTTGACAACAACAAGGCTGGACTTGGCTTCACACCAAATGCACCCGTGAAGATTTCAAGCAAAGTGAAGAAAGCTAGCGCTCAACACATCAGCGTGAGCATTGAACAAGACCAAGAAGAGCCTAAACCCTCCCCTCGGACGTCGGTCTTCGATAGGATGAGCCATTCAAGCTCCAGAACTTCAGTGCTTAATCGCATTGGTGGTCAAGACCGAACCTCTGTCTTCAAGAGGCTTAACGCGCCGACATCCCAAAGCTCTGTTTTTGAAAGGTTGTTAAAGCCTAAGAAACAAAGCAACACAACTATCTCTCCTCTGCGACGATCAGCTTCAGAAAGATTTGAAGATAATGGTAAGTCTTTTGGAAAGAGGAAGACAACACCAAAGGAAGAAAAGCTCGATGGGTTAGGAGAAAAAGATGACGCTCGAAGCTTGATTCCTTCAAGGATGGAGCGCCAAGCAATCCTAGAGGTCGACACAAAAGGACCACTGAAGGTAAGGAGGCGCATCATCGTCCACACTGGCAAATCTTCATGCCAACAAACCCAAGAGAACGGCACTGAAGAGGAGATCCAAGATGTTTTCTACATCACGGTTCAAGAAGGTAAAGAAGACGAAAGCCCGAGGAAATACTCAGAGTCCCTACTCTCCGACCCTGATCCAAGCGGCAGTACAACCATGCAAGTCATGACCACTGGAGCAACTTCAATCGAGGAACAGCTAGCTCAGATGAATGAAGCAATCGCAAAGCTCACACGGACTGTGGAAGAGAAGGACTTGCAGATTGCCGCAGTTGTCAACCAACTAGATGCGCTGCCCGACGTGAAAATCGACCCAAATATTGGCCTATTAAAGAAAGAAGACGACGAAGAAGAGGAGCCACCAGTGGAGAAAGTTGAAGAGAAGCCGAAGCTAGACCAAGCAACGGCATTCATGGGATCTCTCTCTATCCAGCAGCTACAGGAGATGATCGCAAGCACTGTCAAGACACAGTACGAAGGAAGCTCGCATGACTCCGCACTGTACTCAAAGCCTTACTCCAAGAAGATTGATGCTTTGAAGATGCCAAGGGGTTATCAGCCCCCAAAATTCATGCAGTTCGATGGAAAAGGCAACCCGAAGCAACATGTCGCCCATTTCATTGAAACTTGCAACAATGCTGGGACAGAGGGAGACTACCTCGTCAAACAGTTCGTGCGTTCGCTGAAAAGTAACGCTTTTGACTGGTACACTGACCTCGAACCTGAGTCTATCAACAGTTGGGACCAGCTAGAAAAGGAATTCCTCAACCGTTTCTACAGCACTCGTCGTACTGTAAGCATGCTAGAGCTGACCAGCACGAAACAGTGGAAAGATGAACCTGTCATCGACTACATAAATAGATGGCGTTCTTTAAGTCTGGATTGCAAAGATCGGCTTTCTGAAACCTCTGCCATTGAGATGTGCGTTCAAGGCATGCAGTGGGGACTACACTACATCCTTCAAGGCATCAAACCACGAACATTTGAGGAGTTAGCCACCCGCGCCCATGATATGGAGTTAAGCATCACCCGTCATGGGAAGAAAGAACAGATCCCGACTACAAGAATGACAAAGTTTTGGGGCCAAAGGTGGATAAGGCTGCGTGGAAACCCACCAAGGAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

668

Amino Acids

75.66

Weight (kDa)

8.71

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 516 - 605 2.4e-13 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1535
AatII GACGTC 1 cut(s) 500
AccB7I CCANNNNNTGG 2 cut(s) 1196, 1888
AccI GTMKAC 1 cut(s) 828
AccII CGCG 3 cut(s) 312, 596, 1878
AciI CCGC 5 cut(s) 123, 1020, 1143, 1371, 1876
AclWI GGATC 4 cut(s) 919, 1007, 1300, 1921
AcsI RAATTY 2 cut(s) 1442, 1634
AcuI CTGAAG 4 cut(s) 522, 669, 866, 936
AcyI GRCGYC 1 cut(s) 497
AfaI GTAC 5 cut(s) 1027, 1349, 1379, 1583, 1666
AfeI AGCGCT 1 cut(s) 436
AfiI CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1888, 1911
AflIII ACRYGT 1 cut(s) 1480
AjiI CACGTC 1 cut(s) 1177
Alw26I GTCTC 2 cut(s) 317, 1521
AlwI GGATC 4 cut(s) 919, 1007, 1300, 1921
Ama87I CYCGRG 1 cut(s) 976
Aor51HI AGCGCT 1 cut(s) 436
AoxI GGCC 2 cut(s) 1198, 1957
ApeKI GCWGC 3 cut(s) 1168, 1309, 1975
ApoI RAATTY 2 cut(s) 1442, 1634
ArsI GACNNNNNNTTYG 1 cut(s) 38
AspLEI GCGC 7 cut(s) 151, 437, 598, 810, 861, 1168, 1880
AspS9I GGNCC 4 cut(s) 195, 839, 1618, 1957
AsuHPI GGTGA 2 cut(s) 310, 1894
AsuNHI GCTAGC 2 cut(s) 431, 1075
AvaI CYCGRG 1 cut(s) 976
AvaII GGWCC 3 cut(s) 195, 839, 1618
BbsI GAAGAC 5 cut(s) 496, 571, 734, 972, 1221
BbvI GCAGC 3 cut(s) 1155, 1321, 1962
BccI CCATC 4 cut(s) 749, 796, 1451, 1731
BceAI ACGGC 2 cut(s) 925, 1296
BcoDI GTCTC 2 cut(s) 317, 1521
BfaI CTAG 8 cut(s) 243, 432, 821, 1076, 1160, 1268, 1625, 1679
BfmI CTRYAG 3 cut(s) 99, 1313, 1651
BfoI RGCGCY 2 cut(s) 438, 811
BisI GCNGC 5 cut(s) 1021, 1143, 1169, 1310, 1976
BlsI GCNGC 5 cut(s) 1022, 1144, 1170, 1311, 1977
Bme18I GGWCC 3 cut(s) 195, 839, 1618
BmeT110I CYCGRG 1 cut(s) 976
BmgBI CACGTC 1 cut(s) 1177
BmgT120I GGNCC 4 cut(s) 195, 839, 1618, 1957
BmiI GGNNCC 3 cut(s) 1233, 1619, 1958
BmsI GCATC 6 cut(s) 870, 1153, 1396, 1407, 1851, 1908
BmtI GCTAGC 2 cut(s) 435, 1079
BoxI GACNNNNGTC 1 cut(s) 500
BpiI GAAGAC 5 cut(s) 496, 571, 734, 972, 1221
BplI GAGNNNNNCTC 2 cut(s) 1518, 1550
BpmI CTGGAG 3 cut(s) 164, 514, 1071
BsaHI GRCGYC 1 cut(s) 497
BsaJI CCNNGG 4 cut(s) 491, 977, 1421, 1991
BsaXI ACNNNNNCTCC 2 cut(s) 649, 679
Bsc4I CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1888, 1911
Bse1I ACTGG 4 cut(s) 880, 1054, 1238, 1583
BseDI CCNNGG 4 cut(s) 491, 977, 1421, 1991
BseGI GGATG 4 cut(s) 519, 602, 807, 1830
BseLI CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1888, 1911
BseMII CTCAG 3 cut(s) 1002, 1094, 1590
BseNI ACTGG 4 cut(s) 880, 1054, 1238, 1583
BseRI GAGGAG 5 cut(s) 119, 657, 935, 1244, 1877
BseXI GCAGC 3 cut(s) 1155, 1321, 1962
BseYI CCCAGC 1 cut(s) 1514
Bsh1236I CGCG 3 cut(s) 312, 596, 1878
BshFI GGCC 2 cut(s) 1200, 1959
BsiHKCI CYCGRG 1 cut(s) 976
BsiSI CCGG 1 cut(s) 126
BslFI GGGAC 4 cut(s) 979, 1531, 1631, 1833
BslI CCNNNNNNNGG 6 cut(s) 145, 492, 1196, 1279, 1888, 1911
BsmAI GTCTC 2 cut(s) 317, 1521
BsmFI GGGAC 4 cut(s) 979, 1531, 1631, 1833
BsmI GAATGC 1 cut(s) 1283
BsnI GGCC 2 cut(s) 1200, 1959
BsoBI CYCGRG 1 cut(s) 976
Bsp143I GATC 8 cut(s) 307, 677, 924, 1012, 1292, 1323, 1765, 1926
Bsp68I TCGCGA 1 cut(s) 312
BspACI CCGC 5 cut(s) 123, 1020, 1143, 1371, 1876
BspANI GGCC 2 cut(s) 1200, 1959
BspCNI CTCAG 3 cut(s) 1001, 1093, 1591
BspFNI CGCG 3 cut(s) 312, 596, 1878
BspHI TCATGA 2 cut(s) 24, 1041
BspLI GGNNCC 3 cut(s) 1233, 1619, 1958
BspMAI CTGCAG 1 cut(s) 103
BspOI GCTAGC 2 cut(s) 435, 1079
BspPI GGATC 4 cut(s) 919, 1007, 1300, 1921
BspQI GCTCTTC 1 cut(s) 468
BsrI ACTGG 4 cut(s) 880, 1054, 1238, 1583
BssECI CCNNGG 4 cut(s) 491, 977, 1421, 1991
BssMI GATC 8 cut(s) 307, 677, 924, 1012, 1292, 1323, 1765, 1926
BssNI GRCGYC 1 cut(s) 497
BssT1I CCWWGG 2 cut(s) 1421, 1991
Bst6I CTCTTC 6 cut(s) 186, 468, 912, 1116, 1221, 1248
BstACI GRCGYC 1 cut(s) 497
BstC8I GCNNGC 6 cut(s) 433, 1077, 1330, 1361, 1676, 1811
BstDEI CTNAG 5 cut(s) 189, 639, 988, 1080, 1599
BstF5I GGATG 4 cut(s) 519, 602, 807, 1830
BstFNI CGCG 3 cut(s) 312, 596, 1878
BstH2I RGCGCY 2 cut(s) 438, 811
BstHHI GCGC 7 cut(s) 151, 437, 598, 810, 861, 1168, 1880
BstKTI GATC 8 cut(s) 310, 680, 927, 1015, 1295, 1326, 1768, 1929
BstMAI GTCTC 2 cut(s) 317, 1521
BstMBI GATC 8 cut(s) 307, 677, 924, 1012, 1292, 1323, 1765, 1926
BstMWI GCNNNNNNNGC 4 cut(s) 525, 595, 1806, 1877
BstNSI RCATGY 3 cut(s) 1484, 1678, 1813
BstPAI GACNNNNGTC 1 cut(s) 500
BstSFI CTRYAG 3 cut(s) 99, 1313, 1651
BstUI CGCG 3 cut(s) 312, 596, 1878
BstV1I GCAGC 3 cut(s) 1155, 1321, 1962
BstV2I GAAGAC 5 cut(s) 496, 571, 734, 972, 1221
BstX2I RGATCY 3 cut(s) 924, 1292, 1926
BstYI RGATCY 3 cut(s) 924, 1292, 1926
BsuRI GGCC 2 cut(s) 1200, 1959
BtrI CACGTC 1 cut(s) 1177
BtsCI GGATG 4 cut(s) 519, 602, 807, 1830
BtsI GCAGTG 1 cut(s) 1820
BtuMI TCGCGA 1 cut(s) 312
Cac8I GCNNGC 6 cut(s) 433, 1077, 1330, 1361, 1676, 1811
CciI TCATGA 2 cut(s) 24, 1041
CfoI GCGC 7 cut(s) 151, 437, 598, 810, 861, 1168, 1880
Cfr13I GGNCC 4 cut(s) 195, 839, 1618, 1957
CseI GACGC 1 cut(s) 785
Csp6I GTAC 5 cut(s) 1026, 1348, 1378, 1582, 1665
CviQI GTAC 5 cut(s) 1026, 1348, 1378, 1582, 1665
DdeI CTNAG 5 cut(s) 189, 639, 988, 1080, 1599
DpnI GATC 8 cut(s) 309, 679, 926, 1014, 1294, 1325, 1767, 1928
DpnII GATC 8 cut(s) 307, 677, 924, 1012, 1292, 1323, 1765, 1926
DrdI GACNNNNNNGTC 1 cut(s) 1535
DseDI GACNNNNNNGTC 1 cut(s) 1535
Eam1104I CTCTTC 6 cut(s) 186, 468, 912, 1116, 1221, 1248
EarI CTCTTC 6 cut(s) 186, 468, 912, 1116, 1221, 1248
Eco130I CCWWGG 2 cut(s) 1421, 1991
Eco47I GGWCC 3 cut(s) 195, 839, 1618
Eco47III AGCGCT 1 cut(s) 436
Eco57I CTGAAG 4 cut(s) 522, 669, 866, 936
Eco88I CYCGRG 1 cut(s) 976
EcoRI GAATTC 1 cut(s) 1634
EcoT14I CCWWGG 2 cut(s) 1421, 1991
ErhI CCWWGG 2 cut(s) 1421, 1991
FalI AAGNNNNNCTT 4 cut(s) 1555, 1587, 1755, 1787
FaqI GGGAC 4 cut(s) 979, 1531, 1631, 1833
FauI CCCGC 1 cut(s) 1883
FblI GTMKAC 1 cut(s) 828
Fnu4HI GCNGC 5 cut(s) 1021, 1143, 1169, 1310, 1976
FokI GGATG 4 cut(s) 526, 589, 814, 1817
Fsp4HI GCNGC 5 cut(s) 1021, 1143, 1169, 1310, 1976
FspBI CTAG 8 cut(s) 243, 432, 821, 1076, 1160, 1268, 1625, 1679
GlaI GCGC 7 cut(s) 150, 436, 597, 809, 860, 1167, 1879
GluI GCNGC 5 cut(s) 1021, 1143, 1169, 1310, 1976
GsaI CCCAGC 1 cut(s) 1518
GsuI CTGGAG 3 cut(s) 164, 514, 1071
HaeII RGCGCY 2 cut(s) 438, 811
HaeIII GGCC 2 cut(s) 1200, 1959
HapII CCGG 1 cut(s) 126
HgaI GACGC 1 cut(s) 785
HhaI GCGC 7 cut(s) 151, 437, 598, 810, 861, 1168, 1880
Hin1I GRCGYC 1 cut(s) 497
Hin6I GCGC 7 cut(s) 149, 435, 596, 808, 859, 1166, 1878
HinP1I GCGC 7 cut(s) 149, 435, 596, 808, 859, 1166, 1878
HincII GTYRAC 4 cut(s) 298, 361, 829, 1153
HindII GTYRAC 4 cut(s) 298, 361, 829, 1153
HindIII AAGCTT 1 cut(s) 784
HinfI GANTC 6 cut(s) 129, 325, 790, 992, 1367, 1601
HpaII CCGG 1 cut(s) 126
HphI GGTGA 2 cut(s) 310, 1894
Hpy166II GTNNAC 6 cut(s) 298, 361, 829, 871, 1153, 1584
Hpy188I TCNGA 7 cut(s) 66, 495, 688, 991, 1006, 1083, 1777
Hpy8I GTNNAC 6 cut(s) 298, 361, 829, 871, 1153, 1584
Hpy99I CGWCG 5 cut(s) 502, 678, 1178, 1223, 1665
HpyCH4IV ACGT 2 cut(s) 497, 1176
HpyF10VI GCNNNNNNNGC 4 cut(s) 525, 595, 1806, 1877
HpyF3I CTNAG 5 cut(s) 189, 639, 988, 1080, 1599
HpySE526I ACGT 2 cut(s) 497, 1176
Hsp92I GRCGYC 1 cut(s) 497
HspAI GCGC 7 cut(s) 149, 435, 596, 808, 859, 1166, 1878
Kzo9I GATC 8 cut(s) 307, 677, 924, 1012, 1292, 1323, 1765, 1926
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 5 cut(s) 343, 533, 805, 1052, 1231
Lsp1109I GCAGC 3 cut(s) 1155, 1321, 1962
LweI GCATC 6 cut(s) 870, 1153, 1396, 1407, 1851, 1908
MaeI CTAG 8 cut(s) 243, 432, 821, 1076, 1160, 1268, 1625, 1679
MaeII ACGT 2 cut(s) 497, 1176
MaeIII GTNAC 1 cut(s) 1565
MalI GATC 8 cut(s) 309, 679, 926, 1014, 1294, 1325, 1767, 1928
MboI GATC 8 cut(s) 307, 677, 924, 1012, 1292, 1323, 1765, 1926
MfeI CAATTG 1 cut(s) 78
MflI RGATCY 3 cut(s) 924, 1292, 1926
MluCI AATT 3 cut(s) 78, 1442, 1634
MlyI GAGTC 5 cut(s) 123, 319, 1001, 1361, 1610
MmeI TCCRAC 2 cut(s) 209, 1029
MseI TTAA 7 cut(s) 210, 546, 591, 632, 1205, 1748, 1895
MslI CAYNNNNRTG 3 cut(s) 351, 449, 1793
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 1 cut(s) 126
MunI CAATTG 1 cut(s) 78
Mva1269I GAATGC 1 cut(s) 1283
MvnI CGCG 3 cut(s) 312, 596, 1878
MwoI GCNNNNNNNGC 4 cut(s) 525, 595, 1806, 1877
NdeII GATC 8 cut(s) 307, 677, 924, 1012, 1292, 1323, 1765, 1926
NheI GCTAGC 2 cut(s) 431, 1075
NlaIV GGNNCC 3 cut(s) 1233, 1619, 1958
NruI TCGCGA 1 cut(s) 312
NspI RCATGY 3 cut(s) 1484, 1678, 1813
PaeI GCATGC 2 cut(s) 1678, 1813
PagI TCATGA 2 cut(s) 24, 1041
PciI ACATGT 1 cut(s) 1480
PciSI GCTCTTC 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 1182
PctI GAATGC 1 cut(s) 1283
PfeI GAWTC 1 cut(s) 790
PflMI CCANNNNNTGG 2 cut(s) 1196, 1888
PkrI GCNGC 5 cut(s) 1022, 1144, 1170, 1311, 1977
PleI GAGTC 5 cut(s) 123, 319, 1000, 1361, 1609
PpsI GAGTC 5 cut(s) 123, 319, 1000, 1361, 1609
PscI ACATGT 1 cut(s) 1480
PshAI GACNNNNGTC 1 cut(s) 500
PspFI CCCAGC 1 cut(s) 1514
PspN4I GGNNCC 3 cut(s) 1233, 1619, 1958
PspPI GGNCC 4 cut(s) 195, 839, 1618, 1957
PstI CTGCAG 1 cut(s) 103
PsuI RGATCY 3 cut(s) 924, 1292, 1926
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 312
RsaI GTAC 5 cut(s) 1027, 1349, 1379, 1583, 1666
RsaNI GTAC 5 cut(s) 1026, 1348, 1378, 1582, 1665
RseI CAYNNNNRTG 3 cut(s) 351, 449, 1793
SalI GTCGAC 1 cut(s) 827
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 7 cut(s) 210, 546, 591, 632, 1205, 1748, 1895
SatI GCNGC 5 cut(s) 1021, 1143, 1169, 1310, 1976
Sau3AI GATC 8 cut(s) 307, 677, 924, 1012, 1292, 1323, 1765, 1926
Sau96I GGNCC 4 cut(s) 195, 839, 1618, 1957
SchI GAGTC 5 cut(s) 123, 319, 1001, 1361, 1610
SfaNI GCATC 6 cut(s) 870, 1153, 1396, 1407, 1851, 1908
SfcI CTRYAG 3 cut(s) 99, 1313, 1651
SinI GGWCC 3 cut(s) 195, 839, 1618
SmiMI CAYNNNNRTG 3 cut(s) 351, 449, 1793
SphI GCATGC 2 cut(s) 1678, 1813
Sse9I AATT 3 cut(s) 78, 1442, 1634
SsiI CCGC 5 cut(s) 123, 1020, 1143, 1371, 1876
SspI AATATT 1 cut(s) 1195
SspMI CTAG 8 cut(s) 243, 432, 821, 1076, 1160, 1268, 1625, 1679
StyI CCWWGG 2 cut(s) 1421, 1991
TaiI ACGT 2 cut(s) 500, 1179
TaqI TCGA 9 cut(s) 507, 753, 781, 828, 1065, 1185, 1455, 1593, 1722
TaqII GACCGA 3 cut(s) 212, 490, 582
TasI AATT 3 cut(s) 78, 1442, 1634
TatI WGTACW 2 cut(s) 1025, 1377
TauI GCSGC 2 cut(s) 1023, 1145
TfiI GAWTC 1 cut(s) 790
Tru1I TTAA 7 cut(s) 210, 546, 591, 632, 1205, 1748, 1895
Tru9I TTAA 7 cut(s) 210, 546, 591, 632, 1205, 1748, 1895
TseI GCWGC 3 cut(s) 1168, 1309, 1975
TspDTI ATGAA 8 cut(s) 83, 876, 1100, 1104, 1276, 1435, 1483, 1725
TspGWI ACGGA 1 cut(s) 1126
Van91I CCANNNNNTGG 2 cut(s) 1196, 1888
VpaK11BI GGWCC 3 cut(s) 195, 839, 1618
XapI RAATTY 2 cut(s) 1442, 1634
XceI RCATGY 3 cut(s) 1484, 1678, 1813
XmiI GTMKAC 1 cut(s) 828
XspI CTAG 8 cut(s) 243, 432, 821, 1076, 1160, 1268, 1625, 1679
ZraI GACGTC 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.