pycom06g06960

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000128
Physical Location & Seq
Reverse (-)
11282411 .. 11283487
1077 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g06960.1

Sequence Viewer

Length: 1077 bp
ATGCTAGAGCTGACCAGTACGAAACAGTGGAAAGATGAACCTGTCGTCGACTACATAAATAGATGGCGTTCTTTAAGTCTGGATTGCAAAGATCGGCTTTCTGAAACCTCTGCCATTGAGATGTGCGTTCAAGGCATGCAGTGGGGACTACACTACATCCTTCAAGGCATCAAACCACGAACATTTGAGGAGTTAGCCACCCGCGCCCATGATATGGAGTTAAGCATCACTCGTCATGGGAAGAAAGAACAGATCGCCGACTACAAGAATGACAAAGTTTTGGGGCCAAAGGTGGATAAGGCTACGTGGAAACCCACCAAGGAAGCAATGACGGTCAACACAACTCCAGTCAAAATCCATACACGAAGCAAGGCGATTCAAACCGAAGCTTTTCGTGATCAAGAGATACGTAAACGCACTTTGAAGGAGCTTGAGGAGAAGACTTATCCATTCCCTGACTCTGACGTGGTTGCCATGTTGAAAGACTTGCTTGACAAAAAGGTGATCGACCTACCTGAGTGCAAACGGCCAGAAGATATGAACCGTACTGACAGTCCAAGGTACTGTAAATTCCACCGCTTCATTAGTCATCCGACGGAAAAGTGCTTTGTGCTAAAAGATCTCATCATGAAGTTGGCTCAGAAAGGGATCATCGAGCTAGATCTTAATGATGTGGTGAAGTCAAACTATACCACCGTCACTTCTGGCTCTTTGAACTCAAAATCTTCACCTCAACCGCTGGGGGCATGCTCCAAAACCATGTCAGTCAAGTCAAGTGAAGTTGAAGGATGGACTTATGTCACTCCAAAGAAAATGCACAAGAAACATAGGTCTCCTCCACAAGTTCACCAATCGGAAAGGGGGCAAAGCAGCTACCGTCAACCTTCAAAGCTACATGAAAATGTTAAGGATGATGAAATTATGACACAAAGATCGTCCGTTGCCATCACGATGCGCGATTTCTTCCCAAAGACTTCTTCAATCACTCAGTCAAGACTCCTTGCTATGAAGATTGCAAGGAATGCCTCTCTAAGATCGTTTGACAAATTCAAAAAGCTCCTTGTCTGCACGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

359

Amino Acids

41.27

Weight (kDa)

9.45

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 214
AccI GTMKAC 1 cut(s) 48
AccII CGCG 2 cut(s) 204, 957
AciI CCGC 3 cut(s) 202, 577, 737
AclWI GGATC 1 cut(s) 656
AcoI YGGCCR 1 cut(s) 527
AcsI RAATTY 2 cut(s) 569, 1046
AfaI GTAC 3 cut(s) 19, 547, 563
AfiI CCNNNNNNNGG 1 cut(s) 214
AjiI CACGTC 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 961, 993
AluBI AGCT 7 cut(s) 10, 389, 430, 658, 873, 892, 1057
AluI AGCT 7 cut(s) 10, 389, 430, 658, 873, 892, 1057
Alw26I GTCTC 1 cut(s) 837
AlwI GGATC 1 cut(s) 656
AoxI GGCC 2 cut(s) 284, 527
ApeKI GCWGC 1 cut(s) 870
ApoI RAATTY 2 cut(s) 569, 1046
Asp700I GAANNNNTTC 1 cut(s) 390
AspLEI GCGC 2 cut(s) 206, 957
AspS9I GGNCC 1 cut(s) 284
AsuHPI GGTGA 4 cut(s) 514, 688, 720, 839
BauI CACGAG 1 cut(s) 1069
BbsI GAAGAC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 882
BccI CCATC 3 cut(s) 57, 783, 953
BceAI ACGGC 1 cut(s) 542
BclI TGATCA 1 cut(s) 397
BcoDI GTCTC 1 cut(s) 837
BfaI CTAG 2 cut(s) 5, 659
BglII AGATCT 2 cut(s) 619, 661
BisI GCNGC 1 cut(s) 871
BlsI GCNGC 1 cut(s) 872
BmgBI CACGTC 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 1 cut(s) 285
BmsI GCATC 3 cut(s) 177, 234, 942
BoxI GACNNNNGTC 1 cut(s) 797
BpiI GAAGAC 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 452
BsaAI YACGTR 2 cut(s) 306, 410
BsaI GGTCTC 1 cut(s) 837
BsaJI CCNNGG 2 cut(s) 318, 557
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 15, 347
Bse3DI GCAATG 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 318, 557
BseGI GGATG 4 cut(s) 156, 589, 794, 916
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 3 cut(s) 507, 653, 1001
BseNI ACTGG 2 cut(s) 15, 347
BseRI GAGGAG 3 cut(s) 203, 449, 825
BseXI GCAGC 1 cut(s) 882
BseYI CCCAGC 1 cut(s) 739
BsgI GTGCAG 1 cut(s) 1051
Bsh1236I CGCG 2 cut(s) 204, 957
BshFI GGCC 2 cut(s) 286, 529
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 1 cut(s) 837
BsmFI GGGAC 1 cut(s) 159
BsmI GAATGC 1 cut(s) 1027
BsnI GGCC 2 cut(s) 286, 529
Bso31I GGTCTC 1 cut(s) 837
Bsp143I GATC 9 cut(s) 91, 252, 397, 504, 619, 648, 661, 932, 1034
BspACI CCGC 3 cut(s) 202, 577, 737
BspANI GGCC 2 cut(s) 286, 529
BspCNI CTCAG 3 cut(s) 508, 652, 1000
BspFNI CGCG 2 cut(s) 204, 957
BspHI TCATGA 1 cut(s) 627
BspLI GGNNCC 1 cut(s) 285
BspPI GGATC 1 cut(s) 656
BspTNI GGTCTC 1 cut(s) 837
BsrDI GCAATG 1 cut(s) 333
BsrI ACTGG 2 cut(s) 15, 347
BssECI CCNNGG 2 cut(s) 318, 557
BssMI GATC 9 cut(s) 91, 252, 397, 504, 619, 648, 661, 932, 1034
BssSI CACGAG 1 cut(s) 1069
BssT1I CCWWGG 2 cut(s) 318, 557
Bst2BI CACGAG 1 cut(s) 1069
Bst4CI ACNGT 7 cut(s) 27, 334, 545, 554, 566, 697, 878
BstAPI GCANNNNNTGC 1 cut(s) 1022
BstBAI YACGTR 2 cut(s) 306, 410
BstC8I GCNNGC 2 cut(s) 137, 748
BstDEI CTNAG 4 cut(s) 516, 639, 987, 1031
BstF5I GGATG 4 cut(s) 156, 589, 794, 916
BstFNI CGCG 2 cut(s) 204, 957
BstHHI GCGC 2 cut(s) 206, 957
BstKTI GATC 9 cut(s) 94, 255, 400, 507, 622, 651, 664, 935, 1037
BstMAI GTCTC 1 cut(s) 837
BstMBI GATC 9 cut(s) 91, 252, 397, 504, 619, 648, 661, 932, 1034
BstMWI GCNNNNNNNGC 3 cut(s) 132, 203, 1022
BstNSI RCATGY 2 cut(s) 139, 750
BstPAI GACNNNNGTC 1 cut(s) 797
BstSNI TACGTA 1 cut(s) 410
BstUI CGCG 2 cut(s) 204, 957
BstV1I GCAGC 1 cut(s) 882
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 2 cut(s) 619, 661
BstYI RGATCY 2 cut(s) 619, 661
BsuRI GGCC 2 cut(s) 286, 529
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 4 cut(s) 156, 589, 794, 916
BtsI GCAGTG 1 cut(s) 146
BtsIMutI CAGTG 2 cut(s) 32, 146
Cac8I GCNNGC 2 cut(s) 137, 748
CciI TCATGA 1 cut(s) 627
CfoI GCGC 2 cut(s) 206, 957
Cfr13I GGNCC 1 cut(s) 284
Csp6I GTAC 3 cut(s) 18, 546, 562
CviAII CATG 8 cut(s) 136, 209, 236, 475, 628, 747, 760, 896
CviQI GTAC 3 cut(s) 18, 546, 562
DdeI CTNAG 4 cut(s) 516, 639, 987, 1031
DpnI GATC 9 cut(s) 93, 254, 399, 506, 621, 650, 663, 934, 1036
DpnII GATC 9 cut(s) 91, 252, 397, 504, 619, 648, 661, 932, 1034
EaeI YGGCCR 1 cut(s) 527
Eco105I TACGTA 1 cut(s) 410
Eco130I CCWWGG 2 cut(s) 318, 557
Eco31I GGTCTC 1 cut(s) 837
EcoT14I CCWWGG 2 cut(s) 318, 557
ErhI CCWWGG 2 cut(s) 318, 557
FaeI CATG 8 cut(s) 139, 212, 239, 478, 631, 750, 763, 899
FalI AAGNNNNNCTT 4 cut(s) 81, 113, 474, 506
FaqI GGGAC 1 cut(s) 159
FatI CATG 8 cut(s) 135, 208, 235, 474, 627, 746, 759, 895
FauI CCCGC 1 cut(s) 209
FbaI TGATCA 1 cut(s) 397
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 871
FokI GGATG 4 cut(s) 143, 576, 801, 923
Fsp4HI GCNGC 1 cut(s) 871
FspBI CTAG 2 cut(s) 5, 659
GlaI GCGC 2 cut(s) 205, 956
GluI GCNGC 1 cut(s) 871
GsaI CCCAGC 1 cut(s) 743
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 2 cut(s) 286, 529
HhaI GCGC 2 cut(s) 206, 957
Hin1II CATG 8 cut(s) 139, 212, 239, 478, 631, 750, 763, 899
Hin6I GCGC 2 cut(s) 204, 955
HinP1I GCGC 2 cut(s) 204, 955
HincII GTYRAC 3 cut(s) 49, 337, 881
HindII GTYRAC 3 cut(s) 49, 337, 881
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 3 cut(s) 376, 458, 996
HphI GGTGA 4 cut(s) 514, 688, 720, 839
Hpy166II GTNNAC 5 cut(s) 49, 337, 413, 847, 881
Hpy188I TCNGA 5 cut(s) 103, 463, 594, 642, 856
Hpy188III TCNNGA 6 cut(s) 80, 395, 401, 628, 949, 993
Hpy8I GTNNAC 5 cut(s) 49, 337, 413, 847, 881
Hpy99I CGWCG 2 cut(s) 50, 598
HpyAV CCTTC 4 cut(s) 170, 418, 779, 894
HpyCH4III ACNGT 7 cut(s) 27, 334, 545, 554, 566, 697, 878
HpyCH4IV ACGT 3 cut(s) 305, 409, 465
HpyCH4V TGCA 6 cut(s) 87, 139, 522, 817, 1016, 1068
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 203, 1022
HpyF3I CTNAG 4 cut(s) 516, 639, 987, 1031
HpySE526I ACGT 3 cut(s) 305, 409, 465
Hsp92II CATG 8 cut(s) 139, 212, 239, 478, 631, 750, 763, 899
HspAI GCGC 2 cut(s) 204, 955
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 9 cut(s) 91, 252, 397, 504, 619, 648, 661, 932, 1034
LmnI GCTCC 3 cut(s) 427, 755, 1062
LpnPI CCDG 9 cut(s) 28, 54, 65, 360, 468, 528, 543, 690, 725
Lsp1109I GCAGC 1 cut(s) 882
LweI GCATC 3 cut(s) 177, 234, 942
MaeI CTAG 2 cut(s) 5, 659
MaeII ACGT 3 cut(s) 305, 409, 465
MaeIII GTNAC 2 cut(s) 697, 799
MalI GATC 9 cut(s) 93, 254, 399, 506, 621, 650, 663, 934, 1036
MboI GATC 9 cut(s) 91, 252, 397, 504, 619, 648, 661, 932, 1034
MboII GAAGA 7 cut(s) 253, 451, 545, 717, 955, 969, 1021
MflI RGATCY 2 cut(s) 619, 661
MluCI AATT 3 cut(s) 569, 918, 1046
MlyI GAGTC 2 cut(s) 452, 990
MmeI TCCRAC 1 cut(s) 617
MnlI CCTC 6 cut(s) 118, 181, 427, 741, 846, 1036
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 4 cut(s) 74, 221, 666, 906
MslI CAYNNNNRTG 3 cut(s) 119, 900, 950
MspA1I CMGCKG 1 cut(s) 739
Mva1269I GAATGC 1 cut(s) 1027
MvnI CGCG 2 cut(s) 204, 957
MwoI GCNNNNNNNGC 3 cut(s) 132, 203, 1022
NdeII GATC 9 cut(s) 91, 252, 397, 504, 619, 648, 661, 932, 1034
NlaIII CATG 8 cut(s) 139, 212, 239, 478, 631, 750, 763, 899
NlaIV GGNNCC 1 cut(s) 285
NmuCI GTSAC 2 cut(s) 697, 799
NspI RCATGY 2 cut(s) 139, 750
PaeI GCATGC 2 cut(s) 139, 750
PagI TCATGA 1 cut(s) 627
PctI GAATGC 1 cut(s) 1027
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 1 cut(s) 376
PflMI CCANNNNNTGG 1 cut(s) 214
PkrI GCNGC 1 cut(s) 872
PleI GAGTC 2 cut(s) 452, 990
PpsI GAGTC 2 cut(s) 452, 990
Ppu21I YACGTR 2 cut(s) 306, 410
PshAI GACNNNNGTC 1 cut(s) 797
PspFI CCCAGC 1 cut(s) 739
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 1 cut(s) 284
PsuI RGATCY 2 cut(s) 619, 661
RsaI GTAC 3 cut(s) 19, 547, 563
RsaNI GTAC 3 cut(s) 18, 546, 562
RseI CAYNNNNRTG 3 cut(s) 119, 900, 950
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 4 cut(s) 74, 221, 666, 906
SatI GCNGC 1 cut(s) 871
Sau3AI GATC 9 cut(s) 91, 252, 397, 504, 619, 648, 661, 932, 1034
Sau96I GGNCC 1 cut(s) 284
SchI GAGTC 2 cut(s) 452, 990
SfaNI GCATC 3 cut(s) 177, 234, 942
SmiMI CAYNNNNRTG 3 cut(s) 119, 900, 950
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
SnaBI TACGTA 1 cut(s) 410
SphI GCATGC 2 cut(s) 139, 750
Sse9I AATT 3 cut(s) 569, 918, 1046
SsiI CCGC 3 cut(s) 202, 577, 737
SspMI CTAG 2 cut(s) 5, 659
StyI CCWWGG 2 cut(s) 318, 557
TaaI ACNGT 7 cut(s) 27, 334, 545, 554, 566, 697, 878
TaiI ACGT 3 cut(s) 308, 412, 468
TaqI TCGA 3 cut(s) 48, 507, 654
TasI AATT 3 cut(s) 569, 918, 1046
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 4 cut(s) 74, 221, 666, 906
Tru9I TTAA 4 cut(s) 74, 221, 666, 906
TscAI CASTG 2 cut(s) 32, 146
TseFI GTSAC 2 cut(s) 697, 799
TseI GCWGC 1 cut(s) 870
Tsp45I GTSAC 2 cut(s) 697, 799
TspDTI ATGAA 7 cut(s) 51, 554, 571, 644, 912, 930, 1022
TspGWI ACGGA 2 cut(s) 611, 928
TspRI CASTG 2 cut(s) 32, 146
Van91I CCANNNNNTGG 1 cut(s) 214
XapI RAATTY 2 cut(s) 569, 1046
XceI RCATGY 2 cut(s) 139, 750
XmiI GTMKAC 1 cut(s) 48
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 2 cut(s) 5, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.