pycom04g06680

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000128
Physical Location & Seq
Reverse (-)
6895988 .. 6897352
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g06680.1

Sequence Viewer

Length: 1365 bp
ATGATCGCAAGCACTGTCAAGACACAGTACGAAGGAAGCTCGCATGACTCCGTACTGTACTCAAAGCCTTACTCCAAGAAGATTGATGCTTTGAAGATGCCAAGGGGTTATCAGCCCCCAAAATTCATGCAGTTCGATGGAAAAGGCAACCCGAAGCAACATGTCGCCCATTTCATTGAAACTTGCAACAATGCTGGGACAGAGGGAGACTACCTCGTCAAACAGTTCGTGCGTTCACTGAAAAGTAACGCTTTTGACTGGTACACTGACCTCGAACCTGAGTCTATCAACAGTTGGGACCAGCTAGAAAGGGAATTCCTCAACCGTTTCTACAGCACTCGTCGTACTGTAAGCATGCTAGAGCTGACCAGTACGAAACAGTGGAGAGATGAACCTGTCGTCAACTACATAAATAGATGGCGCTCCTTAAGTCTCGATTGCAAAGATCGGCTTTCTGAAACCTCTGCCATTGAGATGTGCGTTCAAGGCATGCACTGGGGGTTACATTACATCCTCCAAGGCATAAAACCAAGAACATTCGAAGAGCTAGCAACTCGTTCCCACGACATGGAGCTGAGTATCGCTAATCACGGGAAAAAGGAGCCGATTACTAACTTCAATAAGGATAAAGTATTCGCCCCAAATGTAGACAAGACCAGGAAGAAGCCCGCTAAGGAAGCTTTCACCGTCAACACTACCCCCATCAAGGCCTCCTCAGCGCCCATCAAGACCTCCTCAGCGCCCATCAAGACCTCCTCAGCGCCCATCAAGATCTCCTCTAAGAAGAAAGCGAAAGAGATAAAGACAAGTGAGCCTTCTCGCACCCAAGACAGATACAAGAGCACCTTGAGAGAGTTGGAACAGAAAACGTACCCTTTTCCTGACTCTGACATGGCTGCCATGTTAGATGACTTGCTGGAAAAAAAGGTGATTGAGCTACCCGAATGCAAGCGTCCTGAAGAAATGAATCGCACAAACGATCCTAGGTACTGCAAGTACCATCGTATTGTGAGCCATCCTGTTGGCAAGTGCTTCGTTCTTAAAGAACTCATCATGAAGCTAGCACAACAAGGGCAAATTGAGCTCGACCTTGAGGACACGGCCGCAACGCATACCACTACAATTGCGTTTGGATCGTTTAATCCTGTGCCTCTCCAAACAACACCTGACCATTCCCGTGAATGCTTAAGTTGCACGGCACCTCCTACACAACCATCACTGGAGAGAAGCGACCAAGATGCACATGCTGATGATGAAGAAGGATGGATATTGGTAACCTACAAAAAGACAAGGGAACTAAGACCACGAGTCATACAGCCAAAGGTGGAACAAAGAAGAAATCACCAAAGCCTAGAAGAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

455

Amino Acids

52.18

Weight (kDa)

8.77

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 76 - 165 2.6e-13 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000128)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28082 FvH4_2g15191 FvH4_2g31621 FvH4_3g07261 FvH4_3g22806 FvH4_3g26703 FvH4_3g26704 FvH4_3g31360 FvH4_4g01161 FvH4_4g22271 FvH4_4g25732 FvH4_5g00242 FvH4_5g28911 FvH4_5g32461 FvH4_6g13112 FvH4_6g53790 FvH4_6g53791 FvH4_7g03661 FvH4_7g04125 FvH4_7g04126
pyrus_communis pycom01g01950 pycom01g01960 pycom01g03260 pycom01g07760 pycom01g11430 pycom01g23980 pycom02g15030 pycom02g18630 pycom03g12320 pycom03g18810 pycom03g19250 pycom04g05010 pycom04g06520 pycom04g06680 pycom04g09630 pycom04g09640 pycom05g06850 pycom05g07540 pycom05g08940 pycom06g04300 pycom06g05750 pycom06g06960 pycom06g19690 pycom07g08750 pycom07g10700 pycom07g10910 pycom07g27480 pycom07g27790 pycom07g27800 pycom08g13620 pycom09g03540 pycom09g03550 pycom09g09870 pycom09g13480 pycom09g14360 pycom09g14890 pycom09g14930 pycom09g15760 pycom10g02190 pycom10g05270 pycom10g06810 pycom10g06830 pycom10g08060 pycom10g08520 pycom11g02350 pycom11g14170 pycom11g15050 pycom11g19270 pycom11g20160 pycom11g22420 pycom12g08620 pycom12g08630 pycom12g10440 pycom12g16590 pycom133g00190 pycom13g20730 pycom13g22230 pycom13g22670 pycom13g23230 pycom13g23250 pycom13g24880 pycom13g25300 pycom13g28040 pycom13g29010 pycom14g07570 pycom14g18650 pycom15g04500 pycom15g09310 pycom15g27210 pycom15g30810 pycom15g34920 pycom16g16040 pycom16g17260 pycom16g17940 pycom16g20990 pycom16g23470 pycom16g24160 pycom16g24720 pycom16g25910 pycom16g25920 pycom16g26370 pycom17g03310 pycom17g12670 pycom17g17530 pycom17g25090 pycom17g27300 pycom17g27310 pycom420g00140 pycom420g00160 pycom420g00320 pycom505g00220 pycom520g00740 pycom520g01010 pycom520g01020 pycom576g00060 pycom675g00040
rosa_laevigata RLG00000022630
rosa_multiflora Rmu_co7993280.1_g000001 Rmu_co8054704.1_g000001 Rmu_co8148394.1_g000001 Rmu_co8243015.1_g000001 Rmu_co8272095.1_g000001 Rmu_co8282359.1_g000001 Rmu_co8334783.1_g000001 Rmu_co8404405.1_g000001 Rmu_sc0000161.1_g000030 Rmu_sc0000418.1_g000026 Rmu_sc0000488.1_g000015 Rmu_sc0000706.1_g000041 Rmu_sc0000720.1_g000013 Rmu_sc0000776.1_g000022 Rmu_sc0000948.1_g000017 Rmu_sc0001167.1_g000045 Rmu_sc0001167.1_g000047 Rmu_sc0001211.1_g000054 Rmu_sc0001241.1_g000019 Rmu_sc0001307.1_g000021 Rmu_sc0001438.1_g000043 Rmu_sc0001468.1_g000032 Rmu_sc0001851.1_g000020 Rmu_sc0002026.1_g000023 Rmu_sc0002811.1_g000016 Rmu_sc0002958.1_g000002 Rmu_sc0002958.1_g000003 Rmu_sc0003745.1_g000010 Rmu_sc0004234.1_g000006 Rmu_sc0004234.1_g000008 Rmu_sc0005035.1_g000001 Rmu_sc0005320.1_g000010 Rmu_sc0005866.1_g000006 Rmu_sc0006139.1_g000009 Rmu_sc0006504.1_g000008 Rmu_sc0006695.1_g000049 Rmu_sc0006695.1_g000050 Rmu_sc0006981.1_g000001 Rmu_sc0007108.1_g000012 Rmu_sc0007355.1_g000001 Rmu_sc0007873.1_g000015 Rmu_sc0007961.1_g000003 Rmu_sc0008991.1_g000005 Rmu_sc0009528.1_g000009 Rmu_sc0013299.1_g000003 Rmu_sc0013532.1_g000015 Rmu_sc0015524.1_g000001 Rmu_sc0015793.1_g000001 Rmu_sc0015817.1_g000006 Rmu_sc0015930.1_g000003 Rmu_sc0015930.1_g000006 Rmu_sc0016952.1_g000002 Rmu_sc0016952.1_g000003 Rmu_sc0018715.1_g000003 Rmu_sc0018715.1_g000005 Rmu_sc0023934.1_g000003 Rmu_sc0023989.1_g000002 Rmu_sc0023989.1_g000003 Rmu_sc0028612.1_g000001 Rmu_sc0029049.1_g000001 Rmu_sc0029639.1_g000001 Rmu_sc0035263.1_g000001 Rmu_sc0037892.1_g000001 Rmu_ssc0000170.1_g000024 Rmu_ssc0000233.1_g000057 Rmu_ssc0000259.1_g000055 Rmu_ssc0000259.1_g000057
rosa_roxburghii Rroxscaffold_4G00287780 Rroxscaffold_4G00325290 Rroxscaffold_5G00333910 Rroxscaffold_7G00211350
rosa_rugosa Rorug01G0056300 Rorug04G0149000 Rorug07G0222000
rosa_samantha Rh1AG072900 Rh1CG072000
rosa_wichuraiana Rw0G003630 Rw0G005250 Rw0G020770 Rw0G023770 Rw1G005460 Rw1G005980 Rw1G007190 Rw1G011350 Rw1G022520 Rw1G028660 Rw2G018620 Rw2G019490 Rw4G006910 Rw4G013020 Rw4G023970 Rw5G044650 Rw6G006590 Rw6G012210 Rw6G023750 Rw7G027370 Rw7G027460 Rw7G035570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AccB1I GGYRCC 1 cut(s) 1198
AccB7I CCANNNNNTGG 1 cut(s) 568
AccI GTMKAC 1 cut(s) 648
AciI CCGC 2 cut(s) 669, 1104
AclWI GGATC 2 cut(s) 974, 1141
AcoI YGGCCR 1 cut(s) 1101
AcsI RAATTY 2 cut(s) 122, 314
AcuI CTGAAG 1 cut(s) 978
AfaI GTAC 9 cut(s) 29, 54, 59, 263, 346, 373, 872, 989, 998
AfiI CCNNNNNNNGG 2 cut(s) 568, 706
AflII CTTAAG 2 cut(s) 427, 1186
AflIII ACRYGT 1 cut(s) 160
AgsI TTSAA 4 cut(s) 94, 179, 485, 619
AhdI GACNNNNNGTC 1 cut(s) 1307
AjnI CCWGG 1 cut(s) 656
AluBI AGCT 9 cut(s) 39, 304, 364, 547, 574, 680, 937, 1060, 1084
AluI AGCT 9 cut(s) 39, 304, 364, 547, 574, 680, 937, 1060, 1084
Alw21I GWGCWC 2 cut(s) 845, 1086
Alw26I GTCTC 2 cut(s) 201, 437
AlwI GGATC 2 cut(s) 974, 1141
AoxI GGCC 2 cut(s) 708, 1101
ApeKI GCWGC 1 cut(s) 896
ApoI RAATTY 2 cut(s) 122, 314
AspA2I CCTAGG 1 cut(s) 983
AspLEI GCGC 4 cut(s) 423, 721, 742, 763
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 3 cut(s) 676, 940, 1334
AsuII TTCGAA 1 cut(s) 540
AsuNHI GCTAGC 2 cut(s) 547, 1060
AvaII GGWCC 1 cut(s) 298
AvrII CCTAGG 1 cut(s) 983
BanI GGYRCC 1 cut(s) 1198
BanII GRGCYC 1 cut(s) 1086
BauI CACGAG 1 cut(s) 1305
Bbv12I GWGCWC 2 cut(s) 845, 1086
BbvCI CCTCAGC 3 cut(s) 715, 736, 757
BbvI GCAGC 1 cut(s) 883
BceAI ACGGC 2 cut(s) 1116, 1212
BcgI CGANNNNNNTGC 4 cut(s) 1015, 1049, 1220, 1254
BciT130I CCWGG 1 cut(s) 658
BcoDI GTCTC 2 cut(s) 201, 437
BfaI CTAG 6 cut(s) 305, 359, 548, 984, 1061, 1352
BfmI CTRYAG 1 cut(s) 331
BfoI RGCGCY 4 cut(s) 424, 722, 743, 764
BfrI CTTAAG 2 cut(s) 427, 1186
BglII AGATCT 1 cut(s) 771
BisI GCNGC 2 cut(s) 897, 1104
BlnI CCTAGG 1 cut(s) 983
BlsI GCNGC 2 cut(s) 898, 1105
Bme1390I CCNGG 1 cut(s) 658
Bme18I GGWCC 1 cut(s) 298
BmeRI GACNNNNNGTC 1 cut(s) 1307
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 3 cut(s) 299, 603, 1200
BmrFI CCNGG 1 cut(s) 658
BmrI ACTGGG 1 cut(s) 505
BmsI GCATC 3 cut(s) 76, 87, 1228
BmtI GCTAGC 2 cut(s) 551, 1064
BmuI ACTGGG 1 cut(s) 505
BplI GAGNNNNNCTC 2 cut(s) 198, 230
BpmI CTGGAG 1 cut(s) 1241
Bpu10I CCTNAGC 4 cut(s) 672, 715, 736, 757
Bpu14I TTCGAA 1 cut(s) 540
BpuEI CTTGAG 2 cut(s) 868, 1112
BsaJI CCNNGG 3 cut(s) 101, 517, 983
BsaXI ACNNNNNCTCC 2 cut(s) 1186, 1216
Bsc4I CCNNNNNNNGG 2 cut(s) 568, 706
Bse1I ACTGG 4 cut(s) 263, 369, 500, 1224
BseBI CCWGG 1 cut(s) 658
BseDI CCNNGG 3 cut(s) 101, 517, 983
BseGI GGATG 3 cut(s) 510, 1015, 1268
BseLI CCNNNNNNNGG 2 cut(s) 568, 706
BseMII CTCAG 5 cut(s) 270, 566, 729, 750, 771
BseNI ACTGG 4 cut(s) 263, 369, 500, 1224
BseRI GAGGAG 4 cut(s) 703, 724, 745, 766
BseX3I CGGCCG 1 cut(s) 1101
BseXI GCAGC 1 cut(s) 883
BseYI CCCAGC 1 cut(s) 194
Bsh1285I CGRYCG 1 cut(s) 1104
BshFI GGCC 2 cut(s) 710, 1103
BshNI GGYRCC 1 cut(s) 1198
BsiEI CGRYCG 1 cut(s) 1104
BsiHKAI GWGCWC 2 cut(s) 845, 1086
BslFI GGGAC 2 cut(s) 211, 311
BslI CCNNNNNNNGG 2 cut(s) 568, 706
BsmAI GTCTC 2 cut(s) 201, 437
BsmFI GGGAC 2 cut(s) 211, 311
BsmI GAATGC 2 cut(s) 950, 1187
BsnI GGCC 2 cut(s) 710, 1103
Bsp119I TTCGAA 1 cut(s) 540
Bsp1286I GDGCHC 2 cut(s) 845, 1086
Bsp143I GATC 5 cut(s) 3, 445, 771, 979, 1133
BspACI CCGC 2 cut(s) 669, 1104
BspANI GGCC 2 cut(s) 710, 1103
BspCNI CTCAG 5 cut(s) 271, 567, 728, 749, 770
BspHI TCATGA 1 cut(s) 1053
BspLI GGNNCC 3 cut(s) 299, 603, 1200
BspOI GCTAGC 2 cut(s) 551, 1064
BspPI GGATC 2 cut(s) 974, 1141
BspQI GCTCTTC 1 cut(s) 537
BspT104I TTCGAA 1 cut(s) 540
BspT107I GGYRCC 1 cut(s) 1198
BspTI CTTAAG 2 cut(s) 427, 1186
BsrI ACTGG 4 cut(s) 263, 369, 500, 1224
BssECI CCNNGG 3 cut(s) 101, 517, 983
BssMI GATC 5 cut(s) 3, 445, 771, 979, 1133
BssSI CACGAG 1 cut(s) 1305
BssT1I CCWWGG 3 cut(s) 101, 517, 983
Bst2BI CACGAG 1 cut(s) 1305
Bst2UI CCWGG 1 cut(s) 658
Bst4CI ACNGT 9 cut(s) 16, 27, 57, 225, 293, 326, 349, 381, 688
Bst6I CTCTTC 1 cut(s) 537
BstAFI CTTAAG 2 cut(s) 427, 1186
BstBI TTCGAA 1 cut(s) 540
BstC8I GCNNGC 8 cut(s) 10, 41, 356, 491, 549, 669, 950, 1062
BstDEI CTNAG 8 cut(s) 279, 575, 672, 715, 736, 757, 780, 1298
BstEII GGTNACC 1 cut(s) 1273
BstF5I GGATG 3 cut(s) 510, 1015, 1268
BstH2I RGCGCY 4 cut(s) 424, 722, 743, 764
BstHHI GCGC 4 cut(s) 423, 721, 742, 763
BstKTI GATC 5 cut(s) 6, 448, 774, 982, 1136
BstMAI GTCTC 2 cut(s) 201, 437
BstMBI GATC 5 cut(s) 3, 445, 771, 979, 1133
BstMCI CGRYCG 1 cut(s) 1104
BstMWI GCNNNNNNNGC 5 cut(s) 486, 677, 716, 1081, 1191
BstNI CCWGG 1 cut(s) 658
BstNSI RCATGY 4 cut(s) 164, 358, 493, 1247
BstPI GGTNACC 1 cut(s) 1273
BstSCI CCNGG 1 cut(s) 656
BstSFI CTRYAG 1 cut(s) 331
BstV1I GCAGC 1 cut(s) 883
BstX2I RGATCY 1 cut(s) 771
BstXI CCANNNNNNTGG 1 cut(s) 1022
BstYI RGATCY 1 cut(s) 771
BstZI CGGCCG 1 cut(s) 1101
BsuRI GGCC 2 cut(s) 710, 1103
BtsCI GGATG 3 cut(s) 510, 1015, 1268
BtsIMutI CAGTG 6 cut(s) 12, 236, 264, 386, 493, 1217
Cac8I GCNNGC 8 cut(s) 10, 41, 356, 491, 549, 669, 950, 1062
CciI TCATGA 1 cut(s) 1053
CfoI GCGC 4 cut(s) 423, 721, 742, 763
Cfr13I GGNCC 1 cut(s) 298
CseI GACGC 1 cut(s) 941
Csp6I GTAC 9 cut(s) 28, 53, 58, 262, 345, 372, 871, 988, 997
CspCI CAANNNNNGTGG 2 cut(s) 1105, 1140
CviQI GTAC 9 cut(s) 28, 53, 58, 262, 345, 372, 871, 988, 997
DdeI CTNAG 8 cut(s) 279, 575, 672, 715, 736, 757, 780, 1298
DpnI GATC 5 cut(s) 5, 447, 773, 981, 1135
DpnII GATC 5 cut(s) 3, 445, 771, 979, 1133
DrdI GACNNNNNNGTC 1 cut(s) 215
DriI GACNNNNNGTC 1 cut(s) 1307
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 1 cut(s) 1101
EagI CGGCCG 1 cut(s) 1101
Eam1104I CTCTTC 1 cut(s) 537
Eam1105I GACNNNNNGTC 1 cut(s) 1307
EarI CTCTTC 1 cut(s) 537
Ecl136II GAGCTC 1 cut(s) 1084
EclXI CGGCCG 1 cut(s) 1101
Eco130I CCWWGG 3 cut(s) 101, 517, 983
Eco147I AGGCCT 1 cut(s) 710
Eco24I GRGCYC 1 cut(s) 1086
Eco47I GGWCC 1 cut(s) 298
Eco52I CGGCCG 1 cut(s) 1101
Eco53kI GAGCTC 1 cut(s) 1084
Eco57I CTGAAG 1 cut(s) 978
Eco91I GGTNACC 1 cut(s) 1273
EcoICRI GAGCTC 1 cut(s) 1084
EcoO65I GGTNACC 1 cut(s) 1273
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 656
EcoT14I CCWWGG 3 cut(s) 101, 517, 983
EcoT38I GRGCYC 1 cut(s) 1086
ErhI CCWWGG 3 cut(s) 101, 517, 983
FalI AAGNNNNNCTT 8 cut(s) 235, 267, 435, 467, 799, 831, 830, 862
FaqI GGGAC 2 cut(s) 211, 311
FauI CCCGC 1 cut(s) 676
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 2 cut(s) 897, 1104
FokI GGATG 3 cut(s) 497, 1002, 1275
FriOI GRGCYC 1 cut(s) 1086
Fsp4HI GCNGC 2 cut(s) 897, 1104
FspBI CTAG 6 cut(s) 305, 359, 548, 984, 1061, 1352
GlaI GCGC 4 cut(s) 422, 720, 741, 762
GluI GCNGC 2 cut(s) 897, 1104
GsaI CCCAGC 1 cut(s) 198
GsuI CTGGAG 1 cut(s) 1241
HaeII RGCGCY 4 cut(s) 424, 722, 743, 764
HaeIII GGCC 2 cut(s) 710, 1103
HgaI GACGC 1 cut(s) 941
HhaI GCGC 4 cut(s) 423, 721, 742, 763
Hin6I GCGC 4 cut(s) 421, 719, 740, 761
HinP1I GCGC 4 cut(s) 421, 719, 740, 761
HincII GTYRAC 2 cut(s) 403, 691
HindII GTYRAC 2 cut(s) 403, 691
HindIII AAGCTT 1 cut(s) 678
HinfI GANTC 5 cut(s) 47, 281, 884, 967, 1308
HphI GGTGA 3 cut(s) 676, 940, 1334
Hpy166II GTNNAC 5 cut(s) 236, 264, 403, 649, 691
Hpy188I TCNGA 2 cut(s) 457, 889
Hpy188III TCNNGA 8 cut(s) 19, 434, 727, 748, 769, 881, 956, 1054
Hpy8I GTNNAC 5 cut(s) 236, 264, 403, 649, 691
Hpy99I CGWCG 1 cut(s) 345
HpyAV CCTTC 3 cut(s) 26, 825, 1253
HpyCH4III ACNGT 9 cut(s) 16, 27, 57, 225, 293, 326, 349, 381, 688
HpyCH4IV ACGT 1 cut(s) 869
HpyCH4V TGCA 8 cut(s) 130, 186, 441, 493, 948, 993, 1194, 1241
HpyF10VI GCNNNNNNNGC 5 cut(s) 486, 677, 716, 1081, 1191
HpyF3I CTNAG 8 cut(s) 279, 575, 672, 715, 736, 757, 780, 1298
HpySE526I ACGT 1 cut(s) 869
HspAI GCGC 4 cut(s) 421, 719, 740, 761
Kzo9I GATC 5 cut(s) 3, 445, 771, 979, 1133
LguI GCTCTTC 1 cut(s) 537
LmnI GCTCC 3 cut(s) 428, 571, 601
Lsp1109I GCAGC 1 cut(s) 883
LweI GCATC 3 cut(s) 76, 87, 1228
MaeI CTAG 6 cut(s) 305, 359, 548, 984, 1061, 1352
MaeII ACGT 1 cut(s) 869
MaeIII GTNAC 3 cut(s) 245, 501, 1273
MalI GATC 5 cut(s) 5, 447, 773, 981, 1135
MboI GATC 5 cut(s) 3, 445, 771, 979, 1133
MboII GAAGA 8 cut(s) 91, 106, 554, 673, 796, 971, 1268, 1347
MfeI CAATTG 1 cut(s) 1122
MflI RGATCY 1 cut(s) 771
MhlI GDGCHC 2 cut(s) 845, 1086
MluCI AATT 4 cut(s) 122, 314, 1077, 1122
MlyI GAGTC 4 cut(s) 41, 290, 878, 1317
MmeI TCCRAC 1 cut(s) 837
MseI TTAA 4 cut(s) 428, 1041, 1140, 1187
MslI CAYNNNNRTG 3 cut(s) 473, 1176, 1248
MspCI CTTAAG 2 cut(s) 427, 1186
MspR9I CCNGG 1 cut(s) 658
MunI CAATTG 1 cut(s) 1122
Mva1269I GAATGC 2 cut(s) 950, 1187
MvaI CCWGG 1 cut(s) 658
MwoI GCNNNNNNNGC 5 cut(s) 486, 677, 716, 1081, 1191
NdeII GATC 5 cut(s) 3, 445, 771, 979, 1133
NheI GCTAGC 2 cut(s) 547, 1060
NlaIV GGNNCC 3 cut(s) 299, 603, 1200
NspI RCATGY 4 cut(s) 164, 358, 493, 1247
NspV TTCGAA 1 cut(s) 540
PaeI GCATGC 2 cut(s) 358, 493
PagI TCATGA 1 cut(s) 1053
PceI AGGCCT 1 cut(s) 710
PciI ACATGT 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 537
PctI GAATGC 2 cut(s) 950, 1187
PfeI GAWTC 1 cut(s) 967
PflMI CCANNNNNTGG 1 cut(s) 568
PkrI GCNGC 2 cut(s) 898, 1105
PleI GAGTC 4 cut(s) 41, 289, 878, 1316
PpsI GAGTC 4 cut(s) 41, 289, 878, 1316
PscI ACATGT 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 1086
Psp6I CCWGG 1 cut(s) 656
PspEI GGTNACC 1 cut(s) 1273
PspFI CCCAGC 1 cut(s) 194
PspGI CCWGG 1 cut(s) 656
PspN4I GGNNCC 3 cut(s) 299, 603, 1200
PspPI GGNCC 1 cut(s) 298
PsuI RGATCY 1 cut(s) 771
RsaI GTAC 9 cut(s) 29, 54, 59, 263, 346, 373, 872, 989, 998
RsaNI GTAC 9 cut(s) 28, 53, 58, 262, 345, 372, 871, 988, 997
RseI CAYNNNNRTG 3 cut(s) 473, 1176, 1248
SacI GAGCTC 1 cut(s) 1086
SapI GCTCTTC 1 cut(s) 537
SaqAI TTAA 4 cut(s) 428, 1041, 1140, 1187
SatI GCNGC 2 cut(s) 897, 1104
Sau3AI GATC 5 cut(s) 3, 445, 771, 979, 1133
Sau96I GGNCC 1 cut(s) 298
SchI GAGTC 4 cut(s) 41, 290, 878, 1317
ScrFI CCNGG 1 cut(s) 658
SduI GDGCHC 2 cut(s) 845, 1086
SfaNI GCATC 3 cut(s) 76, 87, 1228
SfcI CTRYAG 1 cut(s) 331
SfuI TTCGAA 1 cut(s) 540
SinI GGWCC 1 cut(s) 298
SmiMI CAYNNNNRTG 3 cut(s) 473, 1176, 1248
SmlI CTYRAG 4 cut(s) 427, 847, 1091, 1186
SmoI CTYRAG 4 cut(s) 427, 847, 1091, 1186
SphI GCATGC 2 cut(s) 358, 493
Sse9I AATT 4 cut(s) 122, 314, 1077, 1122
SseBI AGGCCT 1 cut(s) 710
SsiI CCGC 2 cut(s) 669, 1104
SspMI CTAG 6 cut(s) 305, 359, 548, 984, 1061, 1352
SstI GAGCTC 1 cut(s) 1086
StuI AGGCCT 1 cut(s) 710
StyD4I CCNGG 1 cut(s) 656
StyI CCWWGG 3 cut(s) 101, 517, 983
TaaI ACNGT 9 cut(s) 16, 27, 57, 225, 293, 326, 349, 381, 688
TaiI ACGT 1 cut(s) 872
TaqI TCGA 5 cut(s) 135, 273, 435, 540, 1086
TasI AATT 4 cut(s) 122, 314, 1077, 1122
TatI WGTACW 1 cut(s) 57
TauI GCSGC 1 cut(s) 1106
TfiI GAWTC 1 cut(s) 967
Tru1I TTAA 4 cut(s) 428, 1041, 1140, 1187
Tru9I TTAA 4 cut(s) 428, 1041, 1140, 1187
TscAI CASTG 6 cut(s) 19, 243, 271, 386, 500, 1224
TseI GCWGC 1 cut(s) 896
TspDTI ATGAA 6 cut(s) 115, 163, 405, 980, 1070, 1269
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 6 cut(s) 19, 243, 271, 386, 500, 1224
Van91I CCANNNNNTGG 1 cut(s) 568
Vha464I CTTAAG 2 cut(s) 427, 1186
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 2 cut(s) 122, 314
XceI RCATGY 4 cut(s) 164, 358, 493, 1247
XmaJI CCTAGG 1 cut(s) 983
XmiI GTMKAC 1 cut(s) 648
XspI CTAG 6 cut(s) 305, 359, 548, 984, 1061, 1352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.