pycom01g00040

Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
Physical Location & Seq
Forward (+)
92063 .. 97568
5506 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g00040.5

Sequence Viewer

Length: 933 bp
ATGGAGGTTGGAGCTGACCTAAAAATGTCCAAACATAGCCTAGAAGTGGATGAACAGCTGTTAATTGAGGAGGAGAAAGAAGACATGCACACGGCAAGGGTAGAACAACCCTTGCCGCAGCCCCCTAAGCCCTCTAAACCACCCACCACCAGTAAGGACATCCCAATTCCATGTTATCTTGATGTTATTCCACCCAATGTCCCTTTTCCTAGCAGGTTTTTGATTCCCAACCAAGAAGAGAGTAAAAAGGACATCGTGGAAGCCTTCCCAAAGGAGCAAAATGCTATTCAAATTCTTGGTGCAACACAAAGAATGATTCAAGAGAAGGTAGTGGCTGGAAAAGATGTAGAAGGCATCAAAGAGGACATATTTGAAACCACAATTCCCAAGGAAGTTGGATTTTATGACACAGGACAAGTCATAACTTTCGAAGGGGTGTTTATTCTGGAGTTCATTTTGGAGCACACAGGTAAGCCGCCTCCCCGAATTTCAATTTTCTTTTATACTAACATGTGGTTAATGATTCAGGCACCCAATCTGGAATTTAAACTATTATCGGATCATTTCAAGTATCACCGCCCATTCAAAGATAAATTCCATAAGGGATGGATTGTTAAATACCCTTGCATTCATTCATTCATGAAACATCTCATTTTCATACACAACACATTACAAACACATTCTCCCTTCCCTAGCCGTGAGCTTCCCATCTCTCCCCTTATAATCCAAACACCATTTTTCCATTCCACACTTCCATCCCCCAAACCAATTATCCCTAGACCTTATGCTATTGGGCCGCAACAAAGAAGGAGAAAGAAGAGAGGAGAAGCCTTGGACGTTTTGGCTATTCAAGCTTGGATTGTTGGAGCGTTTCTAGGTGTATTCAATCTTTGTTTTCAATGTTTAATTTATATTCTCTTTGTTTTGTTGTAA

Protein Analysis

311

Amino Acids

35.73

Weight (kDa)

7.18

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready

Orthologous Genes (Group: OG0000120)

Species Orthologous Gene IDs
malus_domestica MD00G1178700.v1.1 MD00G1192600.v1.1 MD01G1178800.v1.1 MD08G1118400.v1.1 MD11G1121800.v1.1 MD11G1175900.v1.1 MD14G1073500.v1.1 MD16G1171800.v1.1
prunus_persica Prupe.5G057200_v2.0.a1 Prupe.7G095400_v2.0.a1 Prupe.7G095500_v2.0.a1 Prupe.8G037200_v2.0.a1
pyrus_communis pycom01g00040 pycom01g00270 pycom01g00550 pycom01g00730 pycom01g00740 pycom01g00820 pycom01g00960 pycom01g01060 pycom01g01180 pycom01g01580 pycom01g01700 pycom01g01770 pycom01g01970 pycom01g02520 pycom01g03120 pycom01g03510 pycom01g07770 pycom01g07800 pycom01g11420 pycom02g06240 pycom02g13440 pycom02g16920 pycom02g17900 pycom02g18220 pycom02g21560 pycom03g10720 pycom03g11710 pycom03g11930 pycom04g05770 pycom04g07600 pycom04g07920 pycom04g07980 pycom04g08250 pycom04g09780 pycom04g12720 pycom04g13700 pycom05g00180 pycom05g01110 pycom05g05940 pycom05g06250 pycom05g06870 pycom05g08470 pycom05g08920 pycom05g09050 pycom05g09420 pycom05g14090 pycom06g05150 pycom06g05430 pycom06g06950 pycom06g09990 pycom06g15520 pycom07g08780 pycom07g11240 pycom07g11990 pycom07g14400 pycom07g27820 pycom08g07740 pycom08g10980 pycom08g12370 pycom09g03580 pycom09g09700 pycom09g13450 pycom09g14910 pycom10g01230 pycom10g02170 pycom10g06850 pycom10g08040 pycom10g08250 pycom10g10100 pycom10g15420 pycom1125g00010 pycom11g04190 pycom11g14150 pycom11g15090 pycom11g15720 pycom11g16230 pycom11g17180 pycom11g20110 pycom1236g00020 pycom12426g00290 pycom12426g00350 pycom12426g00660 pycom12426g00700 pycom12462g00070 pycom12463g00060 pycom12473g00010 pycom12503g00010 pycom12517g00150 pycom1256g00180 pycom1256g00220 pycom12575g00030 pycom12581g00010 pycom12596g00090 pycom12612g00020 pycom12621g00090 pycom12633g00040 pycom12637g00020 pycom12658g00010 pycom12658g00020 pycom12659g00070 pycom12668g00040 pycom12668g00050 pycom12674g00040 pycom12681g00050 pycom12681g00060 pycom12690g00020 pycom12695g00020 pycom12g08040 pycom12g08660 pycom12g09550 pycom12g09560 pycom13g03230 pycom13g18200 pycom13g19230 pycom13g20140 pycom13g20700 pycom13g22210 pycom13g23170 pycom13g25290 pycom13g25590 pycom13g25980 pycom13g26010 pycom13g26280 pycom13g26350 pycom13g27560 pycom13g27570 pycom13g27680 pycom13g27780 pycom13g28070 pycom13g28140 pycom13g28230 pycom13g28280 pycom13g29100 pycom1498g00010 pycom14g01730 pycom14g08250 pycom14g08660 pycom14g09260 pycom14g18640 pycom15g28640 pycom15g30060 pycom15g35030 pycom15g37780 pycom1604g00160 pycom1609g00100 pycom1681g00030 pycom1683g00020 pycom16g13500 pycom16g17920 pycom16g18140 pycom16g19340 pycom16g20200 pycom16g21370 pycom16g22140 pycom16g23170 pycom16g23450 pycom16g25040 pycom16g25790 pycom1729g00130 pycom1729g00180 pycom1739g00060 pycom1774g00010 pycom17g06230 pycom17g15230 pycom17g16620 pycom17g17340 pycom17g17660 pycom17g17810 pycom17g18150 pycom17g19280 pycom17g25050 pycom17g25100 pycom17g25680 pycom17g25690 pycom1880g00030 pycom1912g00020 pycom2002g00120 pycom2002g00160 pycom2177g00180 pycom2185g00070 pycom2189g00010 pycom2218g00030 pycom2320g00010 pycom2328g00040 pycom2339g00100 pycom2424g00030 pycom2466g00010 pycom2552g00010 pycom2599g00010 pycom2627g00040 pycom2749g00080 pycom2789g00010 pycom3027g00010 pycom394g00080 pycom436g00190 pycom436g00310 pycom436g00350 pycom436g00410 pycom436g00420 pycom436g00430 pycom443g00010 pycom505g00030 pycom520g00140 pycom520g00220 pycom520g00620 pycom520g00650 pycom555g00010 pycom57g00030 pycom608g00020 pycom723g00040 pycom775g00040 pycom808g00350 pycom808g00590 pycom808g00640 pycom889g00030 pycom908g00040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 722
Acc36I ACCTGC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 529
AciI CCGC 4 cut(s) 116, 476, 577, 797
AclWI GGATC 1 cut(s) 567
AcsI RAATTY 4 cut(s) 291, 486, 542, 593
AfiI CCNNNNNNNGG 1 cut(s) 46
AflIII ACRYGT 1 cut(s) 510
AgsI TTSAA 9 cut(s) 290, 320, 374, 492, 568, 586, 851, 886, 899
AluBI AGCT 4 cut(s) 14, 58, 703, 854
AluI AGCT 4 cut(s) 14, 58, 703, 854
Alw21I GWGCWC 1 cut(s) 465
AlwI GGATC 1 cut(s) 567
AoxI GGCC 1 cut(s) 794
ApeKI GCWGC 1 cut(s) 118
ApoI RAATTY 4 cut(s) 291, 486, 542, 593
AspS9I GGNCC 1 cut(s) 794
AsuHPI GGTGA 1 cut(s) 566
AsuII TTCGAA 1 cut(s) 429
BanI GGYRCC 1 cut(s) 529
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 130
BccI CCATC 3 cut(s) 600, 716, 763
BceAI ACGGC 2 cut(s) 108, 681
BfaI CTAG 5 cut(s) 41, 210, 693, 777, 875
BfuAI ACCTGC 1 cut(s) 204
BisI GCNGC 4 cut(s) 116, 119, 476, 797
BlsI GCNGC 4 cut(s) 117, 120, 477, 798
BmgT120I GGNCC 1 cut(s) 794
BmiI GGNNCC 1 cut(s) 531
BmsI GCATC 1 cut(s) 363
BpiI GAAGAC 1 cut(s) 87
BpmI CTGGAG 1 cut(s) 467
Bpu10I CCTNAGC 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 387, 831
BsaXI ACNNNNNCTCC 2 cut(s) 667, 697
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 150
BseDI CCNNGG 2 cut(s) 387, 831
BseGI GGATG 4 cut(s) 55, 159, 611, 755
BseLI CCNNNNNNNGG 1 cut(s) 46
BseNI ACTGG 1 cut(s) 150
BseRI GAGGAG 3 cut(s) 83, 86, 837
BseXI GCAGC 1 cut(s) 130
BshFI GGCC 1 cut(s) 796
BshNI GGYRCC 1 cut(s) 529
BsiHKAI GWGCWC 1 cut(s) 465
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 185
BsmI GAATGC 1 cut(s) 627
BsnI GGCC 1 cut(s) 796
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 1 cut(s) 559
BspACI CCGC 4 cut(s) 116, 476, 577, 797
BspANI GGCC 1 cut(s) 796
BspHI TCATGA 1 cut(s) 639
BspLI GGNNCC 1 cut(s) 531
BspMI ACCTGC 1 cut(s) 204
BspPI GGATC 1 cut(s) 567
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 529
BsrI ACTGG 1 cut(s) 150
BssECI CCNNGG 2 cut(s) 387, 831
BssMI GATC 1 cut(s) 559
BssT1I CCWWGG 2 cut(s) 387, 831
Bst6I CTCTTC 2 cut(s) 231, 812
BstBI TTCGAA 1 cut(s) 429
BstDEI CTNAG 1 cut(s) 126
BstF5I GGATG 4 cut(s) 55, 159, 611, 755
BstKTI GATC 1 cut(s) 562
BstMBI GATC 1 cut(s) 559
BstMWI GCNNNNNNNGC 2 cut(s) 127, 851
BstNSI RCATGY 2 cut(s) 88, 514
BstV1I GCAGC 1 cut(s) 130
BstV2I GAAGAC 1 cut(s) 87
BsuRI GGCC 1 cut(s) 796
BtsCI GGATG 4 cut(s) 55, 159, 611, 755
BveI ACCTGC 1 cut(s) 204
CciI TCATGA 1 cut(s) 639
Cfr13I GGNCC 1 cut(s) 794
CviAII CATG 4 cut(s) 85, 171, 511, 640
DdeI CTNAG 1 cut(s) 126
DpnI GATC 1 cut(s) 561
DpnII GATC 1 cut(s) 559
DraI TTTAAA 1 cut(s) 547
Eam1104I CTCTTC 2 cut(s) 231, 812
EarI CTCTTC 2 cut(s) 231, 812
Eco130I CCWWGG 2 cut(s) 387, 831
EcoT14I CCWWGG 2 cut(s) 387, 831
ErhI CCWWGG 2 cut(s) 387, 831
FaeI CATG 4 cut(s) 88, 174, 514, 643
FaqI GGGAC 1 cut(s) 185
FatI CATG 4 cut(s) 84, 170, 510, 639
Fnu4HI GCNGC 4 cut(s) 116, 119, 476, 797
FokI GGATG 4 cut(s) 62, 146, 618, 742
Fsp4HI GCNGC 4 cut(s) 116, 119, 476, 797
FspBI CTAG 5 cut(s) 41, 210, 693, 777, 875
GluI GCNGC 4 cut(s) 116, 119, 476, 797
GsuI CTGGAG 1 cut(s) 467
HaeIII GGCC 1 cut(s) 796
Hin1II CATG 4 cut(s) 88, 174, 514, 643
HindIII AAGCTT 1 cut(s) 852
HinfI GANTC 3 cut(s) 223, 316, 523
HphI GGTGA 1 cut(s) 566
Hpy188I TCNGA 1 cut(s) 559
Hpy188III TCNNGA 5 cut(s) 179, 320, 446, 539, 640
HpyAV CCTTC 6 cut(s) 274, 319, 344, 425, 697, 801
HpyCH4IV ACGT 1 cut(s) 837
HpyCH4V TGCA 3 cut(s) 88, 302, 627
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 851
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 1 cut(s) 837
Hsp92II CATG 4 cut(s) 88, 174, 514, 643
Kzo9I GATC 1 cut(s) 559
LmnI GCTCC 4 cut(s) 11, 274, 460, 866
LpnPI CCDG 8 cut(s) 163, 199, 321, 396, 431, 453, 512, 524
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 1 cut(s) 363
MaeI CTAG 5 cut(s) 41, 210, 693, 777, 875
MaeII ACGT 1 cut(s) 837
MalI GATC 1 cut(s) 561
MboI GATC 1 cut(s) 559
MboII GAAGA 3 cut(s) 92, 248, 829
MhlI GDGCHC 1 cut(s) 465
MmeI TCCRAC 2 cut(s) 376, 844
MnlI CCTC 6 cut(s) 61, 64, 142, 355, 489, 815
MseI TTAA 5 cut(s) 62, 518, 546, 615, 905
MspA1I CMGCKG 1 cut(s) 58
Mva1269I GAATGC 1 cut(s) 627
MwoI GCNNNNNNNGC 2 cut(s) 127, 851
NdeII GATC 1 cut(s) 559
NlaIII CATG 4 cut(s) 88, 174, 514, 643
NlaIV GGNNCC 1 cut(s) 531
NspI RCATGY 2 cut(s) 88, 514
NspV TTCGAA 1 cut(s) 429
PagI TCATGA 1 cut(s) 639
PciI ACATGT 1 cut(s) 510
PctI GAATGC 1 cut(s) 627
PfeI GAWTC 3 cut(s) 223, 316, 523
PkrI GCNGC 4 cut(s) 117, 120, 477, 798
PscI ACATGT 1 cut(s) 510
PsiI TTATAA 1 cut(s) 722
PspN4I GGNNCC 1 cut(s) 531
PspPI GGNCC 1 cut(s) 794
PvuII CAGCTG 1 cut(s) 58
SaqAI TTAA 5 cut(s) 62, 518, 546, 615, 905
SatI GCNGC 4 cut(s) 116, 119, 476, 797
Sau3AI GATC 1 cut(s) 559
Sau96I GGNCC 1 cut(s) 794
SduI GDGCHC 1 cut(s) 465
SfaNI GCATC 1 cut(s) 363
SfuI TTCGAA 1 cut(s) 429
SsiI CCGC 4 cut(s) 116, 476, 577, 797
SspMI CTAG 5 cut(s) 41, 210, 693, 777, 875
StyI CCWWGG 2 cut(s) 387, 831
TaiI ACGT 1 cut(s) 840
TaqI TCGA 1 cut(s) 429
TauI GCSGC 3 cut(s) 118, 478, 799
TfiI GAWTC 3 cut(s) 223, 316, 523
Tru1I TTAA 5 cut(s) 62, 518, 546, 615, 905
Tru9I TTAA 5 cut(s) 62, 518, 546, 615, 905
TseI GCWGC 1 cut(s) 118
TspDTI ATGAA 7 cut(s) 66, 442, 620, 624, 628, 646, 656
XapI RAATTY 4 cut(s) 291, 486, 542, 593
XceI RCATGY 2 cut(s) 88, 514
XspI CTAG 5 cut(s) 41, 210, 693, 777, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.