pycom520g00220

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0000120
Physical Location & Seq
Forward (+)
200099 .. 203577
3479 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g00220.17

Sequence Viewer

Length: 2736 bp
ATGTTTATTGAGAGTGCACGCTTAACATTCATAGTTATGAACTTATATTCACAAGTAGTGGAGGTTGCTTATAAACAATCGCTAACGAAGGCCTCGTCAACACTTATAGTTTTCATGATGACTTTTGGAGAATGTTGTGAATTGCGTTTAACCCGGGGTGTTGAGTTTCATAATTTATCGAAAGACCAACTGAAAATCATCTTGTATGCAAGTGTAACATGTGTGGAGAAGAACCTTCTAGCTAGCATTCCATCCATATTTTCATCACATTCATATTTACATTTTGCCCTTAATTACAATTTGTGCATTTTAGTTAATCTCATCAAAAACTCAATCCCCCCATATTTTGTTGAGTCTCAAAGTAGATTAGCACCCCTAGTTAATCCCCGGTTAGAACAATCCCTACTTACATCATTACTACAATTCAAATTTTGGCGCCGTTGCCGGGGATTAGCAAAGTTGCTAATCCCTTGGATTGTTCGTTTCTGTTATTTATTAGAGAAGCACAACATGGCACTTGATAATGCTTCTCTTTTGTCACAACTCATGGAAAGGTCCCAAATGCAAAGCGTTGGTACATTTGATCTTCAATCAAGGAATAACGTGTTTTCCAATACTTACAATTCAGATTGGATCGATCATTCAACCTCTATGTGGTGGGAACCTCAACATGTTCAAGAAGAAGGATATTGGCTGCCATATGAGGAGTTCTATTCAAAACTTATGCAGCCACCACAACCTGCCCAAACAAATTCAGGTACATCTTTCTATAATGATATGCTTACTATGTTACTCACCAATTTGTCTCAGGGGTTTGACCACTTGGAAAAGCAAATTCGGCAGATTGCAGAGTTTGTAGGACAATTTTGCGACCAATGCAAACTCCCTAGTTCAACCATTGTAAATCCGAAGGGAGTATTTGAAACCACCAATGCTATCATGTTAAGAAGTGGCAAGGAGGTTGGAACGGACCCTCAACCATCAAATTCAAATCACAAGGAAGATAGATTGCAATTTGAGGAAGAGGAGTTGGACAAAGCCACGGAAAGGGTGGAAACACCTTTGCCACATCCACCTCAAGACCTTAATTTATCCACCACAGGTAAGGTTGTTCCAAATTTCATTATTTCTAACCCCGTTCCAGCAAATATCCCTATTCCTTGCAGGTTCATGAATTCCAAAGAAGAAGAAAGTGAAAAAGACATCTTGGAACCCCTTCCAAAGGTGCAAAGTATGGAATATCAAGAATTCGTTAAAGAGGATATACTTGAGACAACAATTCCCAAAGAAGTTGGATTTTATGACACGGGACAAGTCATAACTCTCAAAATACCAAATCTGTCCAAGGGCAAAGTTCCTGAAACGTTCAAAGAAGATGTGTTTATTCTTGAGTTCATGTCGGAGTACACAGGTAAGCCGCCTCCCCGAATTTCAATTTTCTTTTATATTAATATGTTGTTAATGATTCAGGCATCCAATCTTGAATTTAAACTATTACCGGATCATTTCAAGTATCACCTTCCATTCAAAGATAAATTCCATAAAATAAAAAATAAGATAGGCTGGAGCCTTCACAAAGGTCGTTTATGTGAAGCCCCACTACTTGGCAAAACTACAGGAGCGGGAGGCATCGAAGATGGATCATTCGAGGTGCCTCTAGAAGAAAGCCGCAAGCCTTTGACAGTCACTTTGACGGTCACTTTGAATTCGACCATTAGATTCTTGCAGCTCATCAAGCTTCCTCTTCATACCCGTCGAATAGTTATTTGCAAGCACGTTCAAACTTTTATTCTCGTGCTTAAGCTGATTCAACTTGGCGGGATCGAGCAAGTAGGCGTTGACCCATGTTGGACACAGACGGACCGTCCTGAAAGCAGCCTACTATCTTTAGGAGTGAGGAGGTTCCTAGCTACTATCGTAGCTGTTGTTGCATCCGTCATCATGGAGTCTTCACCTGTAAGAGGACCATTAGAAGATAAGAAAGAAGGGCTTTTTAAAGGTGTTCAAAGAGAAGGGTTGGATGGAGTAAAATCTCAAAGGGCCGGAGACGATTTTCAAGAATGGGCAATCTTCAAAATGTGCATGTTGGAGGTGATCAATACCTCTATAAAAAGCCAAGGCGACACGACCACCGATTCAAAAAGCCGGATTTTCCTGCGTAAAGTTCGGCGACGCTCTCTCGGCTTTTCAGAAAACGCGTTCAACTTTGTCAAAAGATCTCTGACAAAGTTGAAAACACGTGGAGGTCATCATCGCAACTACACACTTTTAAAACGCGTGCAAAAGATCTCTGACAGAGCCGCATCCGCACTACTACCTGCCATTCAGAAATCAAAGAGGCGTTTGTTCAGAAATCGAAGAGGCGCTTGCTCAGAAATCGAAGAGGCGCTTGCTCAGAAATCAAAGAGGCGTTTGTTCAGAAATCGAAGAGGTAACACCCTCCGAACCTCAAAAGTTGAGCCGCTTATTCAAAGATCGAAGAGTCATCACTATTTGAATTTCAAAAAGCCGGATTTGCTGGATCAAAACTTTTGTCGACAAGGATATGTCGACCTTCATCGTTTATGGCAAGGCAGACCTGAAGAAAATGCCCAACTCTTCCTCACCTCTGAGGGCGCACTCCCAGCAAAGCCTCTCGAAATACTCAATTTTTCTTCTTCTTCCCTTGAGAACACCTATTCAACCATTGAAGATACCAGATTATGTGGAGATAAGCGCAACAAAAATATGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve

Protein Analysis

912

Amino Acids

104.45

Weight (kDa)

9.13

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000120)

Species Orthologous Gene IDs
malus_domestica MD00G1178700.v1.1 MD00G1192600.v1.1 MD01G1178800.v1.1 MD08G1118400.v1.1 MD11G1121800.v1.1 MD11G1175900.v1.1 MD14G1073500.v1.1 MD16G1171800.v1.1
prunus_persica Prupe.5G057200_v2.0.a1 Prupe.7G095400_v2.0.a1 Prupe.7G095500_v2.0.a1 Prupe.8G037200_v2.0.a1
pyrus_communis pycom01g00040 pycom01g00270 pycom01g00550 pycom01g00730 pycom01g00740 pycom01g00820 pycom01g00960 pycom01g01060 pycom01g01180 pycom01g01580 pycom01g01700 pycom01g01770 pycom01g01970 pycom01g02520 pycom01g03120 pycom01g03510 pycom01g07770 pycom01g07800 pycom01g11420 pycom02g06240 pycom02g13440 pycom02g16920 pycom02g17900 pycom02g18220 pycom02g21560 pycom03g10720 pycom03g11710 pycom03g11930 pycom04g05770 pycom04g07600 pycom04g07920 pycom04g07980 pycom04g08250 pycom04g09780 pycom04g12720 pycom04g13700 pycom05g00180 pycom05g01110 pycom05g05940 pycom05g06250 pycom05g06870 pycom05g08470 pycom05g08920 pycom05g09050 pycom05g09420 pycom05g14090 pycom06g05150 pycom06g05430 pycom06g06950 pycom06g09990 pycom06g15520 pycom07g08780 pycom07g11240 pycom07g11990 pycom07g14400 pycom07g27820 pycom08g07740 pycom08g10980 pycom08g12370 pycom09g03580 pycom09g09700 pycom09g13450 pycom09g14910 pycom10g01230 pycom10g02170 pycom10g06850 pycom10g08040 pycom10g08250 pycom10g10100 pycom10g15420 pycom1125g00010 pycom11g04190 pycom11g14150 pycom11g15090 pycom11g15720 pycom11g16230 pycom11g17180 pycom11g20110 pycom1236g00020 pycom12426g00290 pycom12426g00350 pycom12426g00660 pycom12426g00700 pycom12462g00070 pycom12463g00060 pycom12473g00010 pycom12503g00010 pycom12517g00150 pycom1256g00180 pycom1256g00220 pycom12575g00030 pycom12581g00010 pycom12596g00090 pycom12612g00020 pycom12621g00090 pycom12633g00040 pycom12637g00020 pycom12658g00010 pycom12658g00020 pycom12659g00070 pycom12668g00040 pycom12668g00050 pycom12674g00040 pycom12681g00050 pycom12681g00060 pycom12690g00020 pycom12695g00020 pycom12g08040 pycom12g08660 pycom12g09550 pycom12g09560 pycom13g03230 pycom13g18200 pycom13g19230 pycom13g20140 pycom13g20700 pycom13g22210 pycom13g23170 pycom13g25290 pycom13g25590 pycom13g25980 pycom13g26010 pycom13g26280 pycom13g26350 pycom13g27560 pycom13g27570 pycom13g27680 pycom13g27780 pycom13g28070 pycom13g28140 pycom13g28230 pycom13g28280 pycom13g29100 pycom1498g00010 pycom14g01730 pycom14g08250 pycom14g08660 pycom14g09260 pycom14g18640 pycom15g28640 pycom15g30060 pycom15g35030 pycom15g37780 pycom1604g00160 pycom1609g00100 pycom1681g00030 pycom1683g00020 pycom16g13500 pycom16g17920 pycom16g18140 pycom16g19340 pycom16g20200 pycom16g21370 pycom16g22140 pycom16g23170 pycom16g23450 pycom16g25040 pycom16g25790 pycom1729g00130 pycom1729g00180 pycom1739g00060 pycom1774g00010 pycom17g06230 pycom17g15230 pycom17g16620 pycom17g17340 pycom17g17660 pycom17g17810 pycom17g18150 pycom17g19280 pycom17g25050 pycom17g25100 pycom17g25680 pycom17g25690 pycom1880g00030 pycom1912g00020 pycom2002g00120 pycom2002g00160 pycom2177g00180 pycom2185g00070 pycom2189g00010 pycom2218g00030 pycom2320g00010 pycom2328g00040 pycom2339g00100 pycom2424g00030 pycom2466g00010 pycom2552g00010 pycom2599g00010 pycom2627g00040 pycom2749g00080 pycom2789g00010 pycom3027g00010 pycom394g00080 pycom436g00190 pycom436g00310 pycom436g00350 pycom436g00410 pycom436g00420 pycom436g00430 pycom443g00010 pycom505g00030 pycom520g00140 pycom520g00220 pycom520g00620 pycom520g00650 pycom555g00010 pycom57g00030 pycom608g00020 pycom723g00040 pycom775g00040 pycom808g00350 pycom808g00590 pycom808g00640 pycom889g00030 pycom908g00040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 72
Acc36I ACCTGC 3 cut(s) 748, 1155, 2326
AccB1I GGYRCC 2 cut(s) 435, 1651
AccB7I CCANNNNNTGG 1 cut(s) 1604
AccBSI CCGCTC 1 cut(s) 1622
AccI GTMKAC 2 cut(s) 2536, 2550
AccII CGCG 2 cut(s) 2198, 2277
AciI CCGC 7 cut(s) 1418, 1622, 1669, 1818, 2301, 2307, 2462
AclI AACGTT 1 cut(s) 1364
AclWI GGATC 5 cut(s) 641, 1509, 1648, 1829, 2529
AcuI CTGAAG 1 cut(s) 2601
AcvI CACGTG 1 cut(s) 2240
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 3 cut(s) 577, 760, 1406
AfiI CCNNNNNNNGG 6 cut(s) 445, 654, 1047, 1222, 1604, 1961
AflII CTTAAG 1 cut(s) 1799
AflIII ACRYGT 6 cut(s) 218, 603, 670, 2196, 2237, 2275
AhdI GACNNNNNGTC 1 cut(s) 1863
AluBI AGCT 6 cut(s) 242, 1729, 1738, 1804, 1910, 1922
AluI AGCT 6 cut(s) 242, 1729, 1738, 1804, 1910, 1922
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 4 cut(s) 360, 810, 1265, 2040
Alw44I GTGCAC 1 cut(s) 15
AlwI GGATC 5 cut(s) 641, 1509, 1648, 1829, 2529
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 2 cut(s) 90, 2040
ApaLI GTGCAC 1 cut(s) 15
ApeKI GCWGC 4 cut(s) 694, 727, 1726, 1875
ArsI GACNNNNNNTTYG 8 cut(s) 80, 112, 1672, 1684, 1704, 1716, 2518, 2550
AseI ATTAAT 1 cut(s) 1449
Asp700I GAANNNNTTC 1 cut(s) 1215
AspLEI GCGC 5 cut(s) 438, 2366, 2389, 2618, 2718
AspS9I GGNCC 5 cut(s) 555, 970, 1861, 1964, 2040
AsuC2I CCSGG 4 cut(s) 154, 155, 388, 446
AsuHPI GGTGA 5 cut(s) 787, 1508, 1944, 2104, 2596
AsuNHI GCTAGC 1 cut(s) 242
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 4 cut(s) 555, 970, 1861, 1964
BaeGI GKGCMC 1 cut(s) 19
BanI GGYRCC 2 cut(s) 435, 1651
BarI GAAGNNNNNNTAC 2 cut(s) 1584, 1616
BauI CACGAG 1 cut(s) 1793
BbrPI CACGTG 1 cut(s) 2240
BbsI GAAGAC 1 cut(s) 1941
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 4 cut(s) 681, 739, 1738, 1887
BccI CCATC 4 cut(s) 259, 988, 1631, 2015
BceAI ACGGC 1 cut(s) 423
BclI TGATCA 1 cut(s) 2094
BcnI CCSGG 4 cut(s) 154, 155, 388, 446
BcoDI GTCTC 4 cut(s) 360, 810, 1265, 2040
BfaI CTAG 6 cut(s) 239, 243, 377, 888, 1658, 1907
BfmI CTRYAG 1 cut(s) 1614
BfoI RGCGCY 3 cut(s) 439, 2367, 2390
BfrI CTTAAG 1 cut(s) 1799
BfuAI ACCTGC 3 cut(s) 748, 1155, 2326
BglII AGATCT 2 cut(s) 2216, 2286
BisI GCNGC 8 cut(s) 695, 728, 1418, 1669, 1727, 1876, 2301, 2462
BlsI GCNGC 8 cut(s) 696, 729, 1419, 1670, 1728, 1877, 2302, 2463
Bme1390I CCNGG 4 cut(s) 154, 155, 388, 446
Bme18I GGWCC 4 cut(s) 555, 970, 1861, 1964
BmeRI GACNNNNNGTC 1 cut(s) 1863
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 5 cut(s) 555, 970, 1861, 1964, 2040
BmiI GGNNCC 8 cut(s) 437, 557, 663, 972, 1212, 1568, 1653, 1904
BmrFI CCNGG 4 cut(s) 154, 155, 388, 446
BmsI GCATC 4 cut(s) 1481, 1638, 1940, 2312
BmtI GCTAGC 1 cut(s) 246
BpiI GAAGAC 1 cut(s) 1941
BpmI CTGGAG 1 cut(s) 1585
BpuEI CTTGAG 4 cut(s) 1062, 1289, 1409, 2687
BpuMI CCSGG 4 cut(s) 154, 155, 388, 446
Bsa29I ATCGAT 1 cut(s) 636
BsaAI YACGTR 1 cut(s) 2240
BsaHI GRCGYC 1 cut(s) 436
BsaJI CCNNGG 8 cut(s) 153, 154, 386, 445, 470, 1041, 1344, 2116
BsaWI WCCGGW 1 cut(s) 1498
Bsc4I CCNNNNNNNGG 6 cut(s) 445, 654, 1047, 1222, 1604, 1961
BseCI ATCGAT 1 cut(s) 636
BseDI CCNNGG 8 cut(s) 153, 154, 386, 445, 470, 1041, 1344, 2116
BseGI GGATG 6 cut(s) 251, 1069, 1472, 1931, 2026, 2303
BseLI CCNNNNNNNGG 6 cut(s) 445, 654, 1047, 1222, 1604, 1961
BseMII CTCAG 4 cut(s) 821, 2385, 2408, 2601
BseRI GAGGAG 3 cut(s) 719, 1040, 1912
BseSI GKGCMC 1 cut(s) 19
BseXI GCAGC 4 cut(s) 681, 739, 1738, 1887
BseYI CCCAGC 1 cut(s) 2623
Bsh1236I CGCG 2 cut(s) 2198, 2277
BshFI GGCC 2 cut(s) 92, 2042
BshNI GGYRCC 2 cut(s) 435, 1651
BshVI ATCGAT 1 cut(s) 636
BsiHKAI GWGCWC 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 7 cut(s) 154, 388, 445, 1499, 2043, 2146, 2510
BslFI GGGAC 2 cut(s) 541, 1323
BslI CCNNNNNNNGG 6 cut(s) 445, 654, 1047, 1222, 1604, 1961
BsmAI GTCTC 4 cut(s) 360, 810, 1265, 2040
BsmBI CGTCTC 1 cut(s) 2040
BsmFI GGGAC 2 cut(s) 541, 1323
BsmI GAATGC 1 cut(s) 246
BsnI GGCC 2 cut(s) 92, 2042
BsoBI CYCGRG 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 19
BspACI CCGC 7 cut(s) 1418, 1622, 1669, 1818, 2301, 2307, 2462
BspANI GGCC 2 cut(s) 92, 2042
BspCNI CTCAG 4 cut(s) 820, 2384, 2407, 2602
BspDI ATCGAT 1 cut(s) 636
BspFNI CGCG 2 cut(s) 2198, 2277
BspHI TCATGA 2 cut(s) 114, 1170
BspLI GGNNCC 8 cut(s) 437, 557, 663, 972, 1212, 1568, 1653, 1904
BspMI ACCTGC 3 cut(s) 748, 1155, 2326
BspOI GCTAGC 1 cut(s) 246
BspPI GGATC 5 cut(s) 641, 1509, 1648, 1829, 2529
BspT107I GGYRCC 2 cut(s) 435, 1651
BspTI CTTAAG 1 cut(s) 1799
BsrBI CCGCTC 1 cut(s) 1622
BssECI CCNNGG 8 cut(s) 153, 154, 386, 445, 470, 1041, 1344, 2116
BssNI GRCGYC 1 cut(s) 436
BssSI CACGAG 1 cut(s) 1793
BssT1I CCWWGG 3 cut(s) 470, 1344, 2116
Bst2BI CACGAG 1 cut(s) 1793
Bst4CI ACNGT 3 cut(s) 1684, 1696, 1865
Bst6I CTCTTC 7 cut(s) 1017, 1749, 2353, 2376, 2422, 2474, 2603
BstACI GRCGYC 1 cut(s) 436
BstAFI CTTAAG 1 cut(s) 1799
BstBAI YACGTR 1 cut(s) 2240
BstC8I GCNNGC 7 cut(s) 19, 244, 1673, 1772, 2279, 2368, 2391
BstDEI CTNAG 4 cut(s) 807, 2371, 2394, 2610
BstDSI CCRYGG 1 cut(s) 1041
BstENI CCTNNNNNAGG 2 cut(s) 1220, 1959
BstF5I GGATG 6 cut(s) 251, 1069, 1472, 1931, 2026, 2303
BstFNI CGCG 2 cut(s) 2198, 2277
BstH2I RGCGCY 3 cut(s) 439, 2367, 2390
BstHHI GCGC 5 cut(s) 438, 2366, 2389, 2618, 2718
BstMAI GTCTC 4 cut(s) 360, 810, 1265, 2040
BstMWI GCNNNNNNNGC 8 cut(s) 838, 876, 1735, 1928, 2181, 2306, 2515, 2624
BstNSI RCATGY 3 cut(s) 222, 674, 2086
BstSCI CCNGG 4 cut(s) 152, 153, 386, 444
BstSFI CTRYAG 1 cut(s) 1614
BstSLI GKGCMC 1 cut(s) 19
BstUI CGCG 2 cut(s) 2198, 2277
BstV1I GCAGC 4 cut(s) 681, 739, 1738, 1887
BstV2I GAAGAC 1 cut(s) 1941
BstX2I RGATCY 2 cut(s) 2216, 2286
BstYI RGATCY 2 cut(s) 2216, 2286
Bsu15I ATCGAT 1 cut(s) 636
BsuRI GGCC 2 cut(s) 92, 2042
BsuTUI ATCGAT 1 cut(s) 636
BtgI CCRYGG 1 cut(s) 1041
BtgZI GCGATG 1 cut(s) 2237
BtsCI GGATG 6 cut(s) 251, 1069, 1472, 1931, 2026, 2303
BveI ACCTGC 3 cut(s) 748, 1155, 2326
Cac8I GCNNGC 7 cut(s) 19, 244, 1673, 1772, 2279, 2368, 2391
CciI TCATGA 2 cut(s) 114, 1170
CfoI GCGC 5 cut(s) 438, 2366, 2389, 2618, 2718
Cfr13I GGNCC 5 cut(s) 555, 970, 1861, 1964, 2040
Cfr9I CCCGGG 1 cut(s) 153
ClaI ATCGAT 1 cut(s) 636
CpoI CGGWCCG 1 cut(s) 1861
CseI GACGC 1 cut(s) 2181
Csp6I GTAC 3 cut(s) 576, 759, 1405
CspI CGGWCCG 1 cut(s) 1861
CviQI GTAC 3 cut(s) 576, 759, 1405
DdeI CTNAG 4 cut(s) 807, 2371, 2394, 2610
DinI GGCGCC 1 cut(s) 437
DraI TTTAAA 3 cut(s) 1489, 1996, 2271
DriI GACNNNNNGTC 1 cut(s) 1863
Eam1104I CTCTTC 7 cut(s) 1017, 1749, 2353, 2376, 2422, 2474, 2603
Eam1105I GACNNNNNGTC 1 cut(s) 1863
EarI CTCTTC 7 cut(s) 1017, 1749, 2353, 2376, 2422, 2474, 2603
Eco130I CCWWGG 3 cut(s) 470, 1344, 2116
Eco147I AGGCCT 1 cut(s) 92
Eco47I GGWCC 4 cut(s) 555, 970, 1861, 1964
Eco57I CTGAAG 1 cut(s) 2601
Eco72I CACGTG 1 cut(s) 2240
Eco88I CYCGRG 1 cut(s) 153
EcoNI CCTNNNNNAGG 2 cut(s) 1220, 1959
EcoO109I RGGNCCY 1 cut(s) 555
EcoRI GAATTC 3 cut(s) 1174, 1247, 1705
EcoT14I CCWWGG 3 cut(s) 470, 1344, 2116
EgeI GGCGCC 1 cut(s) 437
EheI GGCGCC 1 cut(s) 437
ErhI CCWWGG 3 cut(s) 470, 1344, 2116
Esp3I CGTCTC 1 cut(s) 2040
FalI AAGNNNNNCTT 6 cut(s) 1974, 2006, 2350, 2382, 2373, 2405
FaqI GGGAC 2 cut(s) 541, 1323
FauI CCCGC 2 cut(s) 1615, 1811
FauNDI CATATG 1 cut(s) 700
FbaI TGATCA 1 cut(s) 2094
FblI GTMKAC 2 cut(s) 2536, 2550
Fnu4HI GCNGC 8 cut(s) 695, 728, 1418, 1669, 1727, 1876, 2301, 2462
FokI GGATG 6 cut(s) 238, 1056, 1459, 1918, 2033, 2290
Fsp4HI GCNGC 8 cut(s) 695, 728, 1418, 1669, 1727, 1876, 2301, 2462
FspBI CTAG 6 cut(s) 239, 243, 377, 888, 1658, 1907
GlaI GCGC 5 cut(s) 437, 2365, 2388, 2617, 2717
GluI GCNGC 8 cut(s) 695, 728, 1418, 1669, 1727, 1876, 2301, 2462
GsaI CCCAGC 1 cut(s) 2627
GsuI CTGGAG 1 cut(s) 1585
HaeII RGCGCY 3 cut(s) 439, 2367, 2390
HaeIII GGCC 2 cut(s) 92, 2042
HapII CCGG 7 cut(s) 154, 388, 445, 1499, 2043, 2146, 2510
HgaI GACGC 1 cut(s) 2181
HhaI GCGC 5 cut(s) 438, 2366, 2389, 2618, 2718
Hin1I GRCGYC 1 cut(s) 436
Hin6I GCGC 5 cut(s) 436, 2364, 2387, 2616, 2716
HinP1I GCGC 5 cut(s) 436, 2364, 2387, 2616, 2716
HincII GTYRAC 4 cut(s) 99, 1840, 2537, 2551
HindII GTYRAC 4 cut(s) 99, 1840, 2537, 2551
HindIII AAGCTT 1 cut(s) 1736
HinfI GANTC 7 cut(s) 353, 1465, 1719, 1807, 1946, 2135, 2482
HpaII CCGG 7 cut(s) 154, 388, 445, 1499, 2043, 2146, 2510
HphI GGTGA 5 cut(s) 787, 1508, 1944, 2104, 2596
Hpy166II GTNNAC 6 cut(s) 17, 99, 1407, 1840, 2537, 2551
Hpy8I GTNNAC 6 cut(s) 17, 99, 1407, 1840, 2537, 2551
Hpy99I CGWCG 2 cut(s) 1758, 2175
HpyCH4III ACNGT 3 cut(s) 1684, 1696, 1865
HpyCH4IV ACGT 4 cut(s) 603, 1364, 1776, 2239
HpyF10VI GCNNNNNNNGC 8 cut(s) 838, 876, 1735, 1928, 2181, 2306, 2515, 2624
HpyF3I CTNAG 4 cut(s) 807, 2371, 2394, 2610
HpySE526I ACGT 4 cut(s) 603, 1364, 1776, 2239
Hsp92I GRCGYC 1 cut(s) 436
HspAI GCGC 5 cut(s) 436, 2364, 2387, 2616, 2716
KasI GGCGCC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 2094
LmnI GCTCC 2 cut(s) 1566, 1619
Lsp1109I GCAGC 4 cut(s) 681, 739, 1738, 1887
LweI GCATC 4 cut(s) 1481, 1638, 1940, 2312
MaeI CTAG 6 cut(s) 239, 243, 377, 888, 1658, 1907
MaeII ACGT 4 cut(s) 603, 1364, 1776, 2239
MaeIII GTNAC 6 cut(s) 214, 537, 789, 1684, 1696, 2432
MbiI CCGCTC 1 cut(s) 1622
MflI RGATCY 2 cut(s) 2216, 2286
MhlI GDGCHC 1 cut(s) 19
MluI ACGCGT 2 cut(s) 2196, 2275
Mly113I GGCGCC 1 cut(s) 436
MlyI GAGTC 3 cut(s) 362, 1955, 2491
MmeI TCCRAC 7 cut(s) 943, 1011, 1273, 1380, 1829, 1998, 2067
MroXI GAANNNNTTC 1 cut(s) 1215
MslI CAYNNNNRTG 1 cut(s) 35
MspCI CTTAAG 1 cut(s) 1799
MspI CCGG 7 cut(s) 154, 388, 445, 1499, 2043, 2146, 2510
MspR9I CCNGG 4 cut(s) 154, 155, 388, 446
Mva1269I GAATGC 1 cut(s) 246
MvnI CGCG 2 cut(s) 2198, 2277
MwoI GCNNNNNNNGC 8 cut(s) 838, 876, 1735, 1928, 2181, 2306, 2515, 2624
NarI GGCGCC 1 cut(s) 436
NciI CCSGG 4 cut(s) 154, 155, 388, 446
NdeI CATATG 1 cut(s) 700
NheI GCTAGC 1 cut(s) 242
NlaIV GGNNCC 8 cut(s) 437, 557, 663, 972, 1212, 1568, 1653, 1904
NmeAIII GCCGAG 1 cut(s) 2160
NmuCI GTSAC 3 cut(s) 537, 1684, 1696
NspI RCATGY 3 cut(s) 222, 674, 2086
PagI TCATGA 2 cut(s) 114, 1170
PceI AGGCCT 1 cut(s) 92
PciI ACATGT 2 cut(s) 218, 670
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PctI GAATGC 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 1215
PfeI GAWTC 4 cut(s) 1465, 1719, 1807, 2135
PflMI CCANNNNNTGG 1 cut(s) 1604
PkrI GCNGC 8 cut(s) 696, 729, 1419, 1670, 1728, 1877, 2302, 2463
PleI GAGTC 3 cut(s) 361, 1954, 2490
PluTI GGCGCC 1 cut(s) 439
PmaCI CACGTG 1 cut(s) 2240
PmlI CACGTG 1 cut(s) 2240
PpsI GAGTC 3 cut(s) 361, 1954, 2490
Ppu21I YACGTR 1 cut(s) 2240
PpuMI RGGWCCY 1 cut(s) 555
PscI ACATGT 2 cut(s) 218, 670
PshBI ATTAAT 1 cut(s) 1449
PsiI TTATAA 1 cut(s) 72
Psp1406I AACGTT 1 cut(s) 1364
Psp5II RGGWCCY 1 cut(s) 555
PspCI CACGTG 1 cut(s) 2240
PspFI CCCAGC 1 cut(s) 2623
PspN4I GGNNCC 8 cut(s) 437, 557, 663, 972, 1212, 1568, 1653, 1904
PspPI GGNCC 5 cut(s) 555, 970, 1861, 1964, 2040
PspPPI RGGWCCY 1 cut(s) 555
PsrI GAACNNNNNNTAC 2 cut(s) 387, 419
PsuI RGATCY 2 cut(s) 2216, 2286
RsaI GTAC 3 cut(s) 577, 760, 1406
RsaNI GTAC 3 cut(s) 576, 759, 1405
RseI CAYNNNNRTG 1 cut(s) 35
Rsr2I CGGWCCG 1 cut(s) 1861
RsrII CGGWCCG 1 cut(s) 1861
SalI GTCGAC 2 cut(s) 2535, 2549
SatI GCNGC 8 cut(s) 695, 728, 1418, 1669, 1727, 1876, 2301, 2462
Sau96I GGNCC 5 cut(s) 555, 970, 1861, 1964, 2040
SchI GAGTC 3 cut(s) 362, 1955, 2491
ScrFI CCNGG 4 cut(s) 154, 155, 388, 446
SduI GDGCHC 1 cut(s) 19
SfaNI GCATC 4 cut(s) 1481, 1638, 1940, 2312
SfcI CTRYAG 1 cut(s) 1614
SfoI GGCGCC 1 cut(s) 437
SinI GGWCC 4 cut(s) 555, 970, 1861, 1964
SmaI CCCGGG 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 5 cut(s) 1077, 1268, 1388, 1799, 2666
SmoI CTYRAG 5 cut(s) 1077, 1268, 1388, 1799, 2666
SseBI AGGCCT 1 cut(s) 92
SsiI CCGC 7 cut(s) 1418, 1622, 1669, 1818, 2301, 2307, 2462
SspDI GGCGCC 1 cut(s) 435
SspMI CTAG 6 cut(s) 239, 243, 377, 888, 1658, 1907
StuI AGGCCT 1 cut(s) 92
StyD4I CCNGG 4 cut(s) 152, 153, 386, 444
StyI CCWWGG 3 cut(s) 470, 1344, 2116
TaaI ACNGT 3 cut(s) 1684, 1696, 1865
TaiI ACGT 4 cut(s) 606, 1367, 1779, 2242
TatI WGTACW 1 cut(s) 1404
TauI GCSGC 4 cut(s) 1420, 1671, 2303, 2464
TfiI GAWTC 4 cut(s) 1465, 1719, 1807, 2135
TseFI GTSAC 3 cut(s) 537, 1684, 1696
TseI GCWGC 4 cut(s) 694, 727, 1726, 1875
Tsp45I GTSAC 3 cut(s) 537, 1684, 1696
TspGWI ACGGA 4 cut(s) 983, 1058, 1874, 1924
TspMI CCCGGG 1 cut(s) 153
Van91I CCANNNNNTGG 1 cut(s) 1604
Vha464I CTTAAG 1 cut(s) 1799
VneI GTGCAC 1 cut(s) 15
VpaK11BI GGWCC 4 cut(s) 555, 970, 1861, 1964
VspI ATTAAT 1 cut(s) 1449
XagI CCTNNNNNAGG 2 cut(s) 1220, 1959
XbaI TCTAGA 1 cut(s) 1657
XceI RCATGY 3 cut(s) 222, 674, 2086
XcmI CCANNNNNNNNNTGG 1 cut(s) 1048
XmaI CCCGGG 1 cut(s) 153
XmiI GTMKAC 2 cut(s) 2536, 2550
XmnI GAANNNNTTC 1 cut(s) 1215
XspI CTAG 6 cut(s) 239, 243, 377, 888, 1658, 1907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.