Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 217 |
| AciI | CCGC | 2 cut(s) | 164, 265 |
| AclWI | GGATC | 1 cut(s) | 255 |
| AcsI | RAATTY | 3 cut(s) | 174, 230, 281 |
| AflIII | ACRYGT | 1 cut(s) | 198 |
| AgsI | TTSAA | 5 cut(s) | 8, 62, 180, 256, 274 |
| Alw21I | GWGCWC | 1 cut(s) | 153 |
| AlwI | GGATC | 1 cut(s) | 255 |
| ApoI | RAATTY | 3 cut(s) | 174, 230, 281 |
| AsuHPI | GGTGA | 1 cut(s) | 254 |
| AsuII | TTCGAA | 1 cut(s) | 117 |
| BanI | GGYRCC | 1 cut(s) | 217 |
| Bbv12I | GWGCWC | 1 cut(s) | 153 |
| BceAI | ACGGC | 1 cut(s) | 275 |
| BfaI | CTAG | 1 cut(s) | 24 |
| BisI | GCNGC | 1 cut(s) | 164 |
| BlsI | GCNGC | 1 cut(s) | 165 |
| BmiI | GGNNCC | 1 cut(s) | 219 |
| BmsI | GCATC | 1 cut(s) | 51 |
| BpmI | CTGGAG | 1 cut(s) | 155 |
| Bpu14I | TTCGAA | 1 cut(s) | 117 |
| BsaJI | CCNNGG | 1 cut(s) | 75 |
| BsaWI | WCCGGW | 1 cut(s) | 244 |
| BseDI | CCNNGG | 1 cut(s) | 75 |
| BshNI | GGYRCC | 1 cut(s) | 217 |
| BsiHKAI | GWGCWC | 1 cut(s) | 153 |
| BsiSI | CCGG | 1 cut(s) | 245 |
| BslFI | GGGAC | 1 cut(s) | 114 |
| BsmFI | GGGAC | 1 cut(s) | 114 |
| Bsp119I | TTCGAA | 1 cut(s) | 117 |
| Bsp1286I | GDGCHC | 1 cut(s) | 153 |
| Bsp143I | GATC | 1 cut(s) | 247 |
| BspACI | CCGC | 2 cut(s) | 164, 265 |
| BspLI | GGNNCC | 1 cut(s) | 219 |
| BspPI | GGATC | 1 cut(s) | 255 |
| BspT104I | TTCGAA | 1 cut(s) | 117 |
| BspT107I | GGYRCC | 1 cut(s) | 217 |
| BssECI | CCNNGG | 1 cut(s) | 75 |
| BssMI | GATC | 1 cut(s) | 247 |
| BssT1I | CCWWGG | 1 cut(s) | 75 |
| BstBI | TTCGAA | 1 cut(s) | 117 |
| BstKTI | GATC | 1 cut(s) | 250 |
| BstMBI | GATC | 1 cut(s) | 247 |
| BstNSI | RCATGY | 1 cut(s) | 202 |
| CviAII | CATG | 2 cut(s) | 199, 287 |
| CviJI | RGCY | 2 cut(s) | 23, 163 |
| CviKI_1 | RGCY | 2 cut(s) | 23, 163 |
| DpnI | GATC | 1 cut(s) | 249 |
| DpnII | GATC | 1 cut(s) | 247 |
| DraI | TTTAAA | 1 cut(s) | 235 |
| Eco130I | CCWWGG | 1 cut(s) | 75 |
| EcoT14I | CCWWGG | 1 cut(s) | 75 |
| ErhI | CCWWGG | 1 cut(s) | 75 |
| FaeI | CATG | 2 cut(s) | 202, 290 |
| FaiI | YATR | 6 cut(s) | 56, 93, 110, 192, 200, 288 |
| FaqI | GGGAC | 1 cut(s) | 114 |
| FatI | CATG | 2 cut(s) | 198, 286 |
| Fnu4HI | GCNGC | 1 cut(s) | 164 |
| Fsp4HI | GCNGC | 1 cut(s) | 164 |
| FspBI | CTAG | 1 cut(s) | 24 |
| GluI | GCNGC | 1 cut(s) | 164 |
| GsuI | CTGGAG | 1 cut(s) | 155 |
| HapII | CCGG | 1 cut(s) | 245 |
| Hin1II | CATG | 2 cut(s) | 202, 290 |
| HinfI | GANTC | 2 cut(s) | 4, 211 |
| HpaII | CCGG | 1 cut(s) | 245 |
| HphI | GGTGA | 1 cut(s) | 254 |
| Hpy188III | TCNNGA | 3 cut(s) | 8, 134, 227 |
| HpyAV | CCTTC | 3 cut(s) | 7, 32, 113 |
| Hsp92II | CATG | 2 cut(s) | 202, 290 |
| Kzo9I | GATC | 1 cut(s) | 247 |
| LmnI | GCTCC | 1 cut(s) | 148 |
| LpnPI | CCDG | 5 cut(s) | 119, 141, 200, 212, 258 |
| LweI | GCATC | 1 cut(s) | 51 |
| MaeI | CTAG | 1 cut(s) | 24 |
| MalI | GATC | 1 cut(s) | 249 |
| MboI | GATC | 1 cut(s) | 247 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MhlI | GDGCHC | 1 cut(s) | 153 |
| MluCI | AATT | 5 cut(s) | 69, 174, 180, 230, 281 |
| MmeI | TCCRAC | 1 cut(s) | 64 |
| MnlI | CCTC | 2 cut(s) | 43, 177 |
| MseI | TTAA | 2 cut(s) | 206, 234 |
| MspI | CCGG | 1 cut(s) | 245 |
| NdeII | GATC | 1 cut(s) | 247 |
| NlaIII | CATG | 2 cut(s) | 202, 290 |
| NlaIV | GGNNCC | 1 cut(s) | 219 |
| NspI | RCATGY | 1 cut(s) | 202 |
| NspV | TTCGAA | 1 cut(s) | 117 |
| PciI | ACATGT | 1 cut(s) | 198 |
| PfeI | GAWTC | 2 cut(s) | 4, 211 |
| PkrI | GCNGC | 1 cut(s) | 165 |
| PscI | ACATGT | 1 cut(s) | 198 |
| PspN4I | GGNNCC | 1 cut(s) | 219 |
| SaqAI | TTAA | 2 cut(s) | 206, 234 |
| SatI | GCNGC | 1 cut(s) | 164 |
| Sau3AI | GATC | 1 cut(s) | 247 |
| SduI | GDGCHC | 1 cut(s) | 153 |
| SetI | ASST | 2 cut(s) | 18, 160 |
| SfaNI | GCATC | 1 cut(s) | 51 |
| SfuI | TTCGAA | 1 cut(s) | 117 |
| Sse9I | AATT | 5 cut(s) | 69, 174, 180, 230, 281 |
| SsiI | CCGC | 2 cut(s) | 164, 265 |
| SspMI | CTAG | 1 cut(s) | 24 |
| StyI | CCWWGG | 1 cut(s) | 75 |
| TaqI | TCGA | 1 cut(s) | 117 |
| TasI | AATT | 5 cut(s) | 69, 174, 180, 230, 281 |
| TauI | GCSGC | 1 cut(s) | 166 |
| TfiI | GAWTC | 2 cut(s) | 4, 211 |
| Tru1I | TTAA | 2 cut(s) | 206, 234 |
| Tru9I | TTAA | 2 cut(s) | 206, 234 |
| TspDTI | ATGAA | 1 cut(s) | 130 |
| XapI | RAATTY | 3 cut(s) | 174, 230, 281 |
| XceI | RCATGY | 1 cut(s) | 202 |
| XspI | CTAG | 1 cut(s) | 24 |