pycom12690g00020

Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012690
Physical Location & Seq
Reverse (-)
78677 .. 79741
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12690g00020.1

Sequence Viewer

Length: 963 bp
ATGGAGGTTGGAGCTGACCTAAAAATGTCCAAACATAGCCTAGAAGTGGATGAAGAGCTGCTAATTGAGGAGGAGGAAGAAGACATGCACACGGCAAGGGTAGAACAACCCTTGCCGCAGCCCCCTAAGCCCTCTAAACCACCCACCACAAGTAAGGACGTCCCAATTCCATGTTATTCTGATGTTATTCCACCCAATGTCCCTTTTCCTAGCAGGTTTTTGATTCCCAGCCAAGAAGAGAGTAAAAAGGACATCGTGGAAGCCTTCCCAAAGGTGCAAAATGCTATTCCAATTCTTGGTGCAACACAAAGAATGATTCAAGAGAAGGTAGTGGCTGGAGAAGATGTAGAAGGCATCAAAGAGGACATATTTGAAACCACAATTCCCAAGGAAGTTGGATTTTATGACACGGGACAAGTCATAACTTTCGAAGGGGTGTTTATTCTGGAGTTCATTTTGGAGCACACAGGTAAGCCGCCTCCCCGAATTTCAATTTTCTTTTATACTAACATGTGGTTAATGATTCAGGCACCCAATCTGGAATTTAAACTATTACCGATCATTTCAAGTATCACCGCCCATTCAAAGATAAATTCCATGCCGTTTGATTTTATTTATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGTGATACTAAACATGGAGAAAGGTGGAGCTTCACAAAGGTTGTTTGCGTGAAGCCCCACTACGAGCGGAAGCCATCGGAGCGGGAGGCATCGGAGCTAGAGCATTCCAGGCCTCAATACTTGGCCAAGCGCTGGATGACCCAGGAAAAAGGTGCCTCTGGAGATAAGACGCAAGCCTTTGGCGGTCATTGTGAATTCGACCCTCAGATTCTTGCAGCTCATCAAGCTTCCTCTTCATGCCCGCCGAATAGTTATTTGCAAGCACATGCAAACTTCTATTCTCGTACTTAA

Protein Analysis

321

Amino Acids

36.54

Weight (kDa)

8.58

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready

Orthologous Genes (Group: OG0000120)

Species Orthologous Gene IDs
malus_domestica MD00G1178700.v1.1 MD00G1192600.v1.1 MD01G1178800.v1.1 MD08G1118400.v1.1 MD11G1121800.v1.1 MD11G1175900.v1.1 MD14G1073500.v1.1 MD16G1171800.v1.1
prunus_persica Prupe.5G057200_v2.0.a1 Prupe.7G095400_v2.0.a1 Prupe.7G095500_v2.0.a1 Prupe.8G037200_v2.0.a1
pyrus_communis pycom01g00040 pycom01g00270 pycom01g00550 pycom01g00730 pycom01g00740 pycom01g00820 pycom01g00960 pycom01g01060 pycom01g01180 pycom01g01580 pycom01g01700 pycom01g01770 pycom01g01970 pycom01g02520 pycom01g03120 pycom01g03510 pycom01g07770 pycom01g07800 pycom01g11420 pycom02g06240 pycom02g13440 pycom02g16920 pycom02g17900 pycom02g18220 pycom02g21560 pycom03g10720 pycom03g11710 pycom03g11930 pycom04g05770 pycom04g07600 pycom04g07920 pycom04g07980 pycom04g08250 pycom04g09780 pycom04g12720 pycom04g13700 pycom05g00180 pycom05g01110 pycom05g05940 pycom05g06250 pycom05g06870 pycom05g08470 pycom05g08920 pycom05g09050 pycom05g09420 pycom05g14090 pycom06g05150 pycom06g05430 pycom06g06950 pycom06g09990 pycom06g15520 pycom07g08780 pycom07g11240 pycom07g11990 pycom07g14400 pycom07g27820 pycom08g07740 pycom08g10980 pycom08g12370 pycom09g03580 pycom09g09700 pycom09g13450 pycom09g14910 pycom10g01230 pycom10g02170 pycom10g06850 pycom10g08040 pycom10g08250 pycom10g10100 pycom10g15420 pycom1125g00010 pycom11g04190 pycom11g14150 pycom11g15090 pycom11g15720 pycom11g16230 pycom11g17180 pycom11g20110 pycom1236g00020 pycom12426g00290 pycom12426g00350 pycom12426g00660 pycom12426g00700 pycom12462g00070 pycom12463g00060 pycom12473g00010 pycom12503g00010 pycom12517g00150 pycom1256g00180 pycom1256g00220 pycom12575g00030 pycom12581g00010 pycom12596g00090 pycom12612g00020 pycom12621g00090 pycom12633g00040 pycom12637g00020 pycom12658g00010 pycom12658g00020 pycom12659g00070 pycom12668g00040 pycom12668g00050 pycom12674g00040 pycom12681g00050 pycom12681g00060 pycom12690g00020 pycom12695g00020 pycom12g08040 pycom12g08660 pycom12g09550 pycom12g09560 pycom13g03230 pycom13g18200 pycom13g19230 pycom13g20140 pycom13g20700 pycom13g22210 pycom13g23170 pycom13g25290 pycom13g25590 pycom13g25980 pycom13g26010 pycom13g26280 pycom13g26350 pycom13g27560 pycom13g27570 pycom13g27680 pycom13g27780 pycom13g28070 pycom13g28140 pycom13g28230 pycom13g28280 pycom13g29100 pycom1498g00010 pycom14g01730 pycom14g08250 pycom14g08660 pycom14g09260 pycom14g18640 pycom15g28640 pycom15g30060 pycom15g35030 pycom15g37780 pycom1604g00160 pycom1609g00100 pycom1681g00030 pycom1683g00020 pycom16g13500 pycom16g17920 pycom16g18140 pycom16g19340 pycom16g20200 pycom16g21370 pycom16g22140 pycom16g23170 pycom16g23450 pycom16g25040 pycom16g25790 pycom1729g00130 pycom1729g00180 pycom1739g00060 pycom1774g00010 pycom17g06230 pycom17g15230 pycom17g16620 pycom17g17340 pycom17g17660 pycom17g17810 pycom17g18150 pycom17g19280 pycom17g25050 pycom17g25100 pycom17g25680 pycom17g25690 pycom1880g00030 pycom1912g00020 pycom2002g00120 pycom2002g00160 pycom2177g00180 pycom2185g00070 pycom2189g00010 pycom2218g00030 pycom2320g00010 pycom2328g00040 pycom2339g00100 pycom2424g00030 pycom2466g00010 pycom2552g00010 pycom2599g00010 pycom2627g00040 pycom2749g00080 pycom2789g00010 pycom3027g00010 pycom394g00080 pycom436g00190 pycom436g00310 pycom436g00350 pycom436g00410 pycom436g00420 pycom436g00430 pycom443g00010 pycom505g00030 pycom520g00140 pycom520g00220 pycom520g00620 pycom520g00650 pycom555g00010 pycom57g00030 pycom608g00020 pycom723g00040 pycom775g00040 pycom808g00350 pycom808g00590 pycom808g00640 pycom889g00030 pycom908g00040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 162
Acc36I ACCTGC 1 cut(s) 204
AccB1I GGYRCC 2 cut(s) 529, 824
AccB7I CCANNNNNTGG 2 cut(s) 296, 804
AccBSI CCGCTC 2 cut(s) 739, 754
AciI CCGC 7 cut(s) 116, 476, 576, 739, 754, 855, 914
AcoI YGGCCR 1 cut(s) 795
AcsI RAATTY 4 cut(s) 486, 542, 592, 866
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 1 cut(s) 958
AfeI AGCGCT 1 cut(s) 803
AfiI CCNNNNNNNGG 3 cut(s) 46, 296, 804
AflIII ACRYGT 1 cut(s) 510
AgsI TTSAA 5 cut(s) 320, 374, 492, 567, 585
AjnI CCWGG 2 cut(s) 779, 813
AluBI AGCT 6 cut(s) 14, 58, 702, 769, 890, 899
AluI AGCT 6 cut(s) 14, 58, 702, 769, 890, 899
Alw21I GWGCWC 1 cut(s) 465
Aor51HI AGCGCT 1 cut(s) 803
AoxI GGCC 2 cut(s) 782, 795
ApeKI GCWGC 3 cut(s) 58, 118, 887
ApoI RAATTY 4 cut(s) 486, 542, 592, 866
AspLEI GCGC 1 cut(s) 804
AsuHPI GGTGA 1 cut(s) 565
AsuII TTCGAA 1 cut(s) 429
BalI TGGCCA 1 cut(s) 797
BanI GGYRCC 2 cut(s) 529, 824
BarI GAAGNNNNNNTAC 2 cut(s) 716, 748
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 465
BbvI GCAGC 3 cut(s) 45, 130, 899
BccI CCATC 1 cut(s) 754
BceAI ACGGC 2 cut(s) 108, 586
BciT130I CCWGG 2 cut(s) 781, 815
BfaI CTAG 3 cut(s) 41, 210, 770
BfoI RGCGCY 1 cut(s) 805
BfuAI ACCTGC 1 cut(s) 204
BisI GCNGC 5 cut(s) 59, 116, 119, 476, 888
BlsI GCNGC 5 cut(s) 60, 117, 120, 477, 889
Bme1390I CCNGG 2 cut(s) 781, 815
BmiI GGNNCC 2 cut(s) 531, 826
BmrFI CCNGG 2 cut(s) 781, 815
BmsI GCATC 2 cut(s) 363, 770
BpiI GAAGAC 1 cut(s) 87
BpmI CTGGAG 3 cut(s) 357, 467, 852
Bpu10I CCTNAGC 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 387, 813
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 296, 804
BseBI CCWGG 2 cut(s) 781, 815
BseDI CCNNGG 2 cut(s) 387, 813
BseGI GGATG 2 cut(s) 55, 813
BseLI CCNNNNNNNGG 3 cut(s) 46, 296, 804
BseMII CTCAG 1 cut(s) 890
BseRI GAGGAG 2 cut(s) 83, 86
BseXI GCAGC 3 cut(s) 45, 130, 899
BseYI CCCAGC 1 cut(s) 227
BshFI GGCC 2 cut(s) 784, 797
BshNI GGYRCC 2 cut(s) 529, 824
BsiHKAI GWGCWC 1 cut(s) 465
BslFI GGGAC 3 cut(s) 146, 185, 426
BslI CCNNNNNNNGG 3 cut(s) 46, 296, 804
BsmFI GGGAC 3 cut(s) 146, 185, 426
BsmI GAATGC 1 cut(s) 775
BsnI GGCC 2 cut(s) 784, 797
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 1 cut(s) 558
BspACI CCGC 7 cut(s) 116, 476, 576, 739, 754, 855, 914
BspANI GGCC 2 cut(s) 784, 797
BspCNI CTCAG 1 cut(s) 889
BspLI GGNNCC 2 cut(s) 531, 826
BspMI ACCTGC 1 cut(s) 204
BspQI GCTCTTC 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 2 cut(s) 529, 824
BsrBI CCGCTC 2 cut(s) 739, 754
BssECI CCNNGG 2 cut(s) 387, 813
BssMI GATC 1 cut(s) 558
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 2 cut(s) 781, 815
Bst6I CTCTTC 3 cut(s) 48, 231, 910
BstACI GRCGYC 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 3 cut(s) 846, 914, 933
BstDEI CTNAG 2 cut(s) 126, 876
BstF5I GGATG 2 cut(s) 55, 813
BstH2I RGCGCY 1 cut(s) 805
BstHHI GCGC 1 cut(s) 804
BstKTI GATC 1 cut(s) 561
BstMBI GATC 1 cut(s) 558
BstMWI GCNNNNNNNGC 4 cut(s) 127, 751, 781, 896
BstNI CCWGG 2 cut(s) 781, 815
BstNSI RCATGY 3 cut(s) 88, 514, 941
BstSCI CCNGG 2 cut(s) 779, 813
BstV1I GCAGC 3 cut(s) 45, 130, 899
BstV2I GAAGAC 1 cut(s) 87
BsuRI GGCC 2 cut(s) 784, 797
BtsCI GGATG 2 cut(s) 55, 813
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 3 cut(s) 846, 914, 933
CfoI GCGC 1 cut(s) 804
CseI GACGC 1 cut(s) 850
Csp6I GTAC 1 cut(s) 957
CviAII CATG 7 cut(s) 85, 171, 511, 598, 686, 909, 938
CviQI GTAC 1 cut(s) 957
DdeI CTNAG 2 cut(s) 126, 876
DpnI GATC 1 cut(s) 560
DpnII GATC 1 cut(s) 558
DraI TTTAAA 1 cut(s) 547
EaeI YGGCCR 1 cut(s) 795
Eam1104I CTCTTC 3 cut(s) 48, 231, 910
EarI CTCTTC 3 cut(s) 48, 231, 910
Eco130I CCWWGG 1 cut(s) 387
Eco147I AGGCCT 1 cut(s) 784
Eco47III AGCGCT 1 cut(s) 803
EcoRI GAATTC 1 cut(s) 866
EcoRII CCWGG 2 cut(s) 779, 813
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 7 cut(s) 88, 174, 514, 601, 689, 912, 941
FaqI GGGAC 3 cut(s) 146, 185, 426
FatI CATG 7 cut(s) 84, 170, 510, 597, 685, 908, 937
FauI CCCGC 2 cut(s) 747, 921
Fnu4HI GCNGC 5 cut(s) 59, 116, 119, 476, 888
FokI GGATG 2 cut(s) 62, 820
Fsp4HI GCNGC 5 cut(s) 59, 116, 119, 476, 888
FspBI CTAG 3 cut(s) 41, 210, 770
GlaI GCGC 1 cut(s) 803
GluI GCNGC 5 cut(s) 59, 116, 119, 476, 888
GsaI CCCAGC 1 cut(s) 231
GsuI CTGGAG 3 cut(s) 357, 467, 852
HaeII RGCGCY 1 cut(s) 805
HaeIII GGCC 2 cut(s) 784, 797
HgaI GACGC 1 cut(s) 850
HhaI GCGC 1 cut(s) 804
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 7 cut(s) 88, 174, 514, 601, 689, 912, 941
Hin6I GCGC 1 cut(s) 802
HinP1I GCGC 1 cut(s) 802
HindIII AAGCTT 1 cut(s) 897
HinfI GANTC 4 cut(s) 223, 316, 523, 880
HphI GGTGA 1 cut(s) 565
Hpy188I TCNGA 4 cut(s) 181, 751, 766, 879
Hpy188III TCNNGA 4 cut(s) 320, 446, 539, 831
HpyAV CCTTC 4 cut(s) 274, 319, 344, 425
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 6 cut(s) 88, 277, 302, 887, 931, 941
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 751, 781, 896
HpyF3I CTNAG 2 cut(s) 126, 876
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 7 cut(s) 88, 174, 514, 601, 689, 912, 941
HspAI GCGC 1 cut(s) 802
Kzo9I GATC 1 cut(s) 558
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 5 cut(s) 11, 460, 699, 751, 766
Lsp1109I GCAGC 3 cut(s) 45, 130, 899
LweI GCATC 2 cut(s) 363, 770
MaeI CTAG 3 cut(s) 41, 210, 770
MaeII ACGT 1 cut(s) 159
MalI GATC 1 cut(s) 560
MbiI CCGCTC 2 cut(s) 739, 754
MboI GATC 1 cut(s) 558
MboII GAAGA 6 cut(s) 65, 89, 92, 248, 353, 897
MhlI GDGCHC 1 cut(s) 465
MlsI TGGCCA 1 cut(s) 797
MluCI AATT 9 cut(s) 63, 165, 291, 381, 486, 492, 542, 592, 866
MluNI TGGCCA 1 cut(s) 797
MmeI TCCRAC 1 cut(s) 376
Mox20I TGGCCA 1 cut(s) 797
MscI TGGCCA 1 cut(s) 797
MseI TTAA 3 cut(s) 518, 546, 961
Msp20I TGGCCA 1 cut(s) 797
MspR9I CCNGG 2 cut(s) 781, 815
Mva1269I GAATGC 1 cut(s) 775
MvaI CCWGG 2 cut(s) 781, 815
MwoI GCNNNNNNNGC 4 cut(s) 127, 751, 781, 896
NdeII GATC 1 cut(s) 558
NlaIII CATG 7 cut(s) 88, 174, 514, 601, 689, 912, 941
NlaIV GGNNCC 2 cut(s) 531, 826
NspI RCATGY 3 cut(s) 88, 514, 941
NspV TTCGAA 1 cut(s) 429
PceI AGGCCT 1 cut(s) 784
PciI ACATGT 1 cut(s) 510
PciSI GCTCTTC 1 cut(s) 48
PctI GAATGC 1 cut(s) 775
PfeI GAWTC 4 cut(s) 223, 316, 523, 880
PflMI CCANNNNNTGG 2 cut(s) 296, 804
PkrI GCNGC 5 cut(s) 60, 117, 120, 477, 889
PscI ACATGT 1 cut(s) 510
Psp6I CCWGG 2 cut(s) 779, 813
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 2 cut(s) 779, 813
PspN4I GGNNCC 2 cut(s) 531, 826
RsaI GTAC 1 cut(s) 958
RsaNI GTAC 1 cut(s) 957
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 3 cut(s) 518, 546, 961
SatI GCNGC 5 cut(s) 59, 116, 119, 476, 888
Sau3AI GATC 1 cut(s) 558
ScrFI CCNGG 2 cut(s) 781, 815
SduI GDGCHC 1 cut(s) 465
SfaNI GCATC 2 cut(s) 363, 770
SfuI TTCGAA 1 cut(s) 429
Sse9I AATT 9 cut(s) 63, 165, 291, 381, 486, 492, 542, 592, 866
SseBI AGGCCT 1 cut(s) 784
SsiI CCGC 7 cut(s) 116, 476, 576, 739, 754, 855, 914
SspMI CTAG 3 cut(s) 41, 210, 770
StuI AGGCCT 1 cut(s) 784
StyD4I CCNGG 2 cut(s) 779, 813
StyI CCWWGG 1 cut(s) 387
TaiI ACGT 1 cut(s) 162
TaqI TCGA 2 cut(s) 429, 870
TasI AATT 9 cut(s) 63, 165, 291, 381, 486, 492, 542, 592, 866
TauI GCSGC 2 cut(s) 118, 478
TfiI GAWTC 4 cut(s) 223, 316, 523, 880
Tru1I TTAA 3 cut(s) 518, 546, 961
Tru9I TTAA 3 cut(s) 518, 546, 961
TseI GCWGC 3 cut(s) 58, 118, 887
TspDTI ATGAA 3 cut(s) 66, 442, 897
Van91I CCANNNNNTGG 2 cut(s) 296, 804
XapI RAATTY 4 cut(s) 486, 542, 592, 866
XceI RCATGY 3 cut(s) 88, 514, 941
XspI CTAG 3 cut(s) 41, 210, 770
ZraI GACGTC 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.