Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 204 |
| Acc36I | ACCTGC | 1 cut(s) | 245 |
| AciI | CCGC | 1 cut(s) | 158 |
| AcyI | GRCGYC | 1 cut(s) | 201 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 88 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 230, 262 |
| AluBI | AGCT | 2 cut(s) | 56, 100 |
| AluI | AGCT | 2 cut(s) | 56, 100 |
| ApeKI | GCWGC | 2 cut(s) | 100, 160 |
| BbsI | GAAGAC | 1 cut(s) | 129 |
| BbvI | GCAGC | 2 cut(s) | 87, 172 |
| BceAI | ACGGC | 1 cut(s) | 150 |
| BfaI | CTAG | 2 cut(s) | 83, 251 |
| BfuAI | ACCTGC | 1 cut(s) | 245 |
| BisI | GCNGC | 3 cut(s) | 101, 158, 161 |
| BlsI | GCNGC | 3 cut(s) | 102, 159, 162 |
| BpiI | GAAGAC | 1 cut(s) | 129 |
| Bpu10I | CCTNAGC | 1 cut(s) | 168 |
| BsaHI | GRCGYC | 1 cut(s) | 201 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 88 |
| Bse1I | ACTGG | 1 cut(s) | 192 |
| BseGI | GGATG | 1 cut(s) | 97 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 88 |
| BseNI | ACTGG | 1 cut(s) | 192 |
| BseRI | GAGGAG | 2 cut(s) | 125, 128 |
| BseXI | GCAGC | 2 cut(s) | 87, 172 |
| BslFI | GGGAC | 1 cut(s) | 188 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 88 |
| BsmFI | GGGAC | 1 cut(s) | 188 |
| BspACI | CCGC | 1 cut(s) | 158 |
| BspMI | ACCTGC | 1 cut(s) | 245 |
| BspQI | GCTCTTC | 1 cut(s) | 90 |
| BsrI | ACTGG | 1 cut(s) | 192 |
| BssNI | GRCGYC | 1 cut(s) | 201 |
| Bst6I | CTCTTC | 1 cut(s) | 90 |
| BstACI | GRCGYC | 1 cut(s) | 201 |
| BstDEI | CTNAG | 1 cut(s) | 168 |
| BstF5I | GGATG | 1 cut(s) | 97 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 169 |
| BstNSI | RCATGY | 1 cut(s) | 130 |
| BstV1I | GCAGC | 2 cut(s) | 87, 172 |
| BstV2I | GAAGAC | 1 cut(s) | 129 |
| BtsCI | GGATG | 1 cut(s) | 97 |
| BveI | ACCTGC | 1 cut(s) | 245 |
| CviAII | CATG | 2 cut(s) | 127, 213 |
| CviJI | RGCY | 5 cut(s) | 56, 81, 100, 163, 172 |
| CviKI_1 | RGCY | 5 cut(s) | 56, 81, 100, 163, 172 |
| DdeI | CTNAG | 1 cut(s) | 168 |
| Eam1104I | CTCTTC | 1 cut(s) | 90 |
| EarI | CTCTTC | 1 cut(s) | 90 |
| FaeI | CATG | 2 cut(s) | 130, 216 |
| FaiI | YATR | 5 cut(s) | 6, 44, 78, 128, 214 |
| FaqI | GGGAC | 1 cut(s) | 188 |
| FatI | CATG | 2 cut(s) | 126, 212 |
| Fnu4HI | GCNGC | 3 cut(s) | 101, 158, 161 |
| FokI | GGATG | 1 cut(s) | 104 |
| Fsp4HI | GCNGC | 3 cut(s) | 101, 158, 161 |
| FspBI | CTAG | 2 cut(s) | 83, 251 |
| GluI | GCNGC | 3 cut(s) | 101, 158, 161 |
| Hin1I | GRCGYC | 1 cut(s) | 201 |
| Hin1II | CATG | 2 cut(s) | 130, 216 |
| Hpy188I | TCNGA | 1 cut(s) | 223 |
| HpyCH4IV | ACGT | 1 cut(s) | 201 |
| HpyCH4V | TGCA | 1 cut(s) | 130 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 169 |
| HpyF3I | CTNAG | 1 cut(s) | 168 |
| HpySE526I | ACGT | 1 cut(s) | 201 |
| Hsp92I | GRCGYC | 1 cut(s) | 201 |
| Hsp92II | CATG | 2 cut(s) | 130, 216 |
| LguI | GCTCTTC | 1 cut(s) | 90 |
| LmnI | GCTCC | 1 cut(s) | 53 |
| LpnPI | CCDG | 2 cut(s) | 205, 240 |
| Lsp1109I | GCAGC | 2 cut(s) | 87, 172 |
| MaeI | CTAG | 2 cut(s) | 83, 251 |
| MaeII | ACGT | 1 cut(s) | 201 |
| MboII | GAAGA | 3 cut(s) | 107, 131, 134 |
| MluCI | AATT | 2 cut(s) | 105, 207 |
| MmeI | TCCRAC | 1 cut(s) | 31 |
| MnlI | CCTC | 5 cut(s) | 40, 103, 106, 109, 184 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 169 |
| NlaIII | CATG | 2 cut(s) | 130, 216 |
| NspI | RCATGY | 1 cut(s) | 130 |
| PciSI | GCTCTTC | 1 cut(s) | 90 |
| PkrI | GCNGC | 3 cut(s) | 102, 159, 162 |
| SapI | GCTCTTC | 1 cut(s) | 90 |
| SatI | GCNGC | 3 cut(s) | 101, 158, 161 |
| SetI | ASST | 6 cut(s) | 51, 58, 63, 102, 204, 259 |
| SgeI | CNNG | 7 cut(s) | 95, 139, 145, 150, 166, 204, 225 |
| Sse9I | AATT | 2 cut(s) | 105, 207 |
| SsiI | CCGC | 1 cut(s) | 158 |
| SspMI | CTAG | 2 cut(s) | 83, 251 |
| TaiI | ACGT | 1 cut(s) | 204 |
| TasI | AATT | 2 cut(s) | 105, 207 |
| TauI | GCSGC | 1 cut(s) | 160 |
| TseI | GCWGC | 2 cut(s) | 100, 160 |
| TspDTI | ATGAA | 1 cut(s) | 108 |
| XceI | RCATGY | 1 cut(s) | 130 |
| XspI | CTAG | 2 cut(s) | 83, 251 |
| ZraI | GACGTC | 1 cut(s) | 202 |