Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 228 |
| AatII | GACGTC | 1 cut(s) | 765 |
| Acc36I | ACCTGC | 1 cut(s) | 807 |
| AccB1I | GGYRCC | 1 cut(s) | 1222 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 899, 1297 |
| AciI | CCGC | 1 cut(s) | 719 |
| AclWI | GGATC | 1 cut(s) | 1290 |
| AcoI | YGGCCR | 1 cut(s) | 1089 |
| AcsI | RAATTY | 9 cut(s) | 205, 234, 250, 408, 513, 932, 1046, 1177, 1235 |
| AcyI | GRCGYC | 1 cut(s) | 762 |
| AfaI | GTAC | 1 cut(s) | 952 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 20, 649, 899, 1297 |
| AluBI | AGCT | 2 cut(s) | 617, 661 |
| AluI | AGCT | 2 cut(s) | 617, 661 |
| Alw21I | GWGCWC | 1 cut(s) | 28 |
| AlwI | GGATC | 1 cut(s) | 1290 |
| AoxI | GGCC | 1 cut(s) | 1089 |
| ApeKI | GCWGC | 5 cut(s) | 136, 169, 343, 661, 721 |
| ApoI | RAATTY | 9 cut(s) | 205, 234, 250, 408, 513, 932, 1046, 1177, 1235 |
| AspA2I | CCTAGG | 1 cut(s) | 1304 |
| AspS9I | GGNCC | 1 cut(s) | 1301 |
| AsuHPI | GGTGA | 2 cut(s) | 735, 1259 |
| AvaII | GGWCC | 1 cut(s) | 1301 |
| AvrII | CCTAGG | 1 cut(s) | 1304 |
| BalI | TGGCCA | 1 cut(s) | 1091 |
| BanI | GGYRCC | 1 cut(s) | 1222 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1153, 1185 |
| BbsI | GAAGAC | 1 cut(s) | 690 |
| Bbv12I | GWGCWC | 1 cut(s) | 28 |
| BbvI | GCAGC | 5 cut(s) | 148, 181, 355, 648, 733 |
| BccI | CCATC | 2 cut(s) | 917, 1082 |
| BceAI | ACGGC | 2 cut(s) | 711, 1280 |
| BclI | TGATCA | 1 cut(s) | 79 |
| BfaI | CTAG | 4 cut(s) | 644, 813, 1232, 1305 |
| BfuAI | ACCTGC | 1 cut(s) | 807 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 287 |
| BisI | GCNGC | 6 cut(s) | 137, 170, 344, 662, 719, 722 |
| BlnI | CCTAGG | 1 cut(s) | 1304 |
| BlsI | GCNGC | 6 cut(s) | 138, 171, 345, 663, 720, 723 |
| Bme18I | GGWCC | 1 cut(s) | 1301 |
| BmgT120I | GGNCC | 1 cut(s) | 1301 |
| BmiI | GGNNCC | 2 cut(s) | 1224, 1302 |
| BmsI | GCATC | 1 cut(s) | 1011 |
| BpiI | GAAGAC | 1 cut(s) | 690 |
| BsaBI | GATNNNNATC | 1 cut(s) | 228 |
| BsaHI | GRCGYC | 1 cut(s) | 762 |
| BsaJI | CCNNGG | 4 cut(s) | 282, 393, 1296, 1304 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 20, 649, 899, 1297 |
| Bse118I | RCCGGY | 1 cut(s) | 1249 |
| Bse8I | GATNNNNATC | 1 cut(s) | 228 |
| BseDI | CCNNGG | 4 cut(s) | 282, 393, 1296, 1304 |
| BseGI | GGATG | 1 cut(s) | 658 |
| BseJI | GATNNNNATC | 1 cut(s) | 228 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 20, 649, 899, 1297 |
| BseRI | GAGGAG | 6 cut(s) | 161, 335, 491, 671, 686, 689 |
| BseXI | GCAGC | 5 cut(s) | 148, 181, 355, 648, 733 |
| BsgI | GTGCAG | 1 cut(s) | 281 |
| BshFI | GGCC | 1 cut(s) | 1091 |
| BshNI | GGYRCC | 1 cut(s) | 1222 |
| BsiHKAI | GWGCWC | 1 cut(s) | 28 |
| BsiSI | CCGG | 1 cut(s) | 1250 |
| BslFI | GGGAC | 2 cut(s) | 749, 788 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 20, 649, 899, 1297 |
| BsmFI | GGGAC | 2 cut(s) | 749, 788 |
| BsnI | GGCC | 1 cut(s) | 1091 |
| Bsp1286I | GDGCHC | 1 cut(s) | 28 |
| Bsp1407I | TGTACA | 1 cut(s) | 950 |
| Bsp143I | GATC | 2 cut(s) | 79, 1282 |
| Bsp19I | CCATGG | 1 cut(s) | 393 |
| BspACI | CCGC | 1 cut(s) | 719 |
| BspANI | GGCC | 1 cut(s) | 1091 |
| BspHI | TCATGA | 1 cut(s) | 504 |
| BspLI | GGNNCC | 2 cut(s) | 1224, 1302 |
| BspMI | ACCTGC | 1 cut(s) | 807 |
| BspPI | GGATC | 1 cut(s) | 1290 |
| BspT107I | GGYRCC | 1 cut(s) | 1222 |
| BsrFI | RCCGGY | 1 cut(s) | 1249 |
| BsrGI | TGTACA | 1 cut(s) | 950 |
| BssAI | RCCGGY | 1 cut(s) | 1249 |
| BssECI | CCNNGG | 4 cut(s) | 282, 393, 1296, 1304 |
| BssMI | GATC | 2 cut(s) | 79, 1282 |
| BssNI | GRCGYC | 1 cut(s) | 762 |
| BssT1I | CCWWGG | 3 cut(s) | 282, 393, 1304 |
| Bst6I | CTCTTC | 1 cut(s) | 834 |
| BstACI | GRCGYC | 1 cut(s) | 762 |
| BstAUI | TGTACA | 1 cut(s) | 950 |
| BstDSI | CCRYGG | 2 cut(s) | 393, 1296 |
| BstF5I | GGATG | 1 cut(s) | 658 |
| BstKTI | GATC | 2 cut(s) | 82, 1285 |
| BstMBI | GATC | 2 cut(s) | 79, 1282 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 287, 487 |
| BstNSI | RCATGY | 2 cut(s) | 691, 1083 |
| BstV1I | GCAGC | 5 cut(s) | 148, 181, 355, 648, 733 |
| BstV2I | GAAGAC | 1 cut(s) | 690 |
| BsuRI | GGCC | 1 cut(s) | 1091 |
| BtgI | CCRYGG | 2 cut(s) | 393, 1296 |
| BtsCI | GGATG | 1 cut(s) | 658 |
| BveI | ACCTGC | 1 cut(s) | 807 |
| CciI | TCATGA | 1 cut(s) | 504 |
| Cfr10I | RCCGGY | 1 cut(s) | 1249 |
| Cfr13I | GGNCC | 1 cut(s) | 1301 |
| CseI | GACGC | 1 cut(s) | 442 |
| Csp6I | GTAC | 1 cut(s) | 951 |
| CviAII | CATG | 6 cut(s) | 394, 505, 688, 774, 1080, 1292 |
| CviQI | GTAC | 1 cut(s) | 951 |
| DpnI | GATC | 2 cut(s) | 81, 1284 |
| DpnII | GATC | 2 cut(s) | 79, 1282 |
| EaeI | YGGCCR | 1 cut(s) | 1089 |
| Eam1104I | CTCTTC | 1 cut(s) | 834 |
| EarI | CTCTTC | 1 cut(s) | 834 |
| Eco130I | CCWWGG | 3 cut(s) | 282, 393, 1304 |
| Eco47I | GGWCC | 1 cut(s) | 1301 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1301 |
| EcoRI | GAATTC | 2 cut(s) | 932, 1235 |
| EcoT14I | CCWWGG | 3 cut(s) | 282, 393, 1304 |
| ErhI | CCWWGG | 3 cut(s) | 282, 393, 1304 |
| FaeI | CATG | 6 cut(s) | 397, 508, 691, 777, 1083, 1295 |
| FaqI | GGGAC | 2 cut(s) | 749, 788 |
| FatI | CATG | 6 cut(s) | 393, 504, 687, 773, 1079, 1291 |
| FauNDI | CATATG | 3 cut(s) | 142, 186, 316 |
| FbaI | TGATCA | 1 cut(s) | 79 |
| Fnu4HI | GCNGC | 6 cut(s) | 137, 170, 344, 662, 719, 722 |
| FokI | GGATG | 1 cut(s) | 665 |
| Fsp4HI | GCNGC | 6 cut(s) | 137, 170, 344, 662, 719, 722 |
| FspBI | CTAG | 4 cut(s) | 644, 813, 1232, 1305 |
| GluI | GCNGC | 6 cut(s) | 137, 170, 344, 662, 719, 722 |
| HaeIII | GGCC | 1 cut(s) | 1091 |
| HapII | CCGG | 1 cut(s) | 1250 |
| HgaI | GACGC | 1 cut(s) | 442 |
| Hin1I | GRCGYC | 1 cut(s) | 762 |
| Hin1II | CATG | 6 cut(s) | 397, 508, 691, 777, 1083, 1295 |
| HinfI | GANTC | 5 cut(s) | 104, 779, 826, 967, 1216 |
| HpaII | CCGG | 1 cut(s) | 1250 |
| HphI | GGTGA | 2 cut(s) | 735, 1259 |
| Hpy166II | GTNNAC | 4 cut(s) | 62, 530, 953, 1255 |
| Hpy188I | TCNGA | 1 cut(s) | 784 |
| Hpy188III | TCNNGA | 4 cut(s) | 452, 505, 518, 971 |
| Hpy8I | GTNNAC | 4 cut(s) | 62, 530, 953, 1255 |
| HpyAV | CCTTC | 5 cut(s) | 553, 877, 970, 992, 1280 |
| HpyCH4IV | ACGT | 1 cut(s) | 762 |
| HpyCH4V | TGCA | 9 cut(s) | 4, 169, 190, 262, 343, 691, 880, 905, 1083 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 287, 487 |
| HpySE526I | ACGT | 1 cut(s) | 762 |
| Hsp92I | GRCGYC | 1 cut(s) | 762 |
| Hsp92II | CATG | 6 cut(s) | 397, 508, 691, 777, 1083, 1295 |
| Ksp22I | TGATCA | 1 cut(s) | 79 |
| Kzo9I | GATC | 2 cut(s) | 79, 1282 |
| LmnI | GCTCC | 4 cut(s) | 31, 478, 614, 658 |
| LpnPI | CCDG | 7 cut(s) | 248, 422, 802, 1073, 1119, 1205, 1263 |
| Lsp1109I | GCAGC | 5 cut(s) | 148, 181, 355, 648, 733 |
| LweI | GCATC | 1 cut(s) | 1011 |
| MaeI | CTAG | 4 cut(s) | 644, 813, 1232, 1305 |
| MaeII | ACGT | 1 cut(s) | 762 |
| MaeIII | GTNAC | 1 cut(s) | 267 |
| MalI | GATC | 2 cut(s) | 81, 1284 |
| MboI | GATC | 2 cut(s) | 79, 1282 |
| MboII | GAAGA | 8 cut(s) | 575, 692, 695, 851, 928, 1001, 1161, 1164 |
| MhlI | GDGCHC | 1 cut(s) | 28 |
| MlsI | TGGCCA | 1 cut(s) | 1091 |
| MluNI | TGGCCA | 1 cut(s) | 1091 |
| MmeI | TCCRAC | 3 cut(s) | 561, 592, 1128 |
| Mox20I | TGGCCA | 1 cut(s) | 1091 |
| MscI | TGGCCA | 1 cut(s) | 1091 |
| MseI | TTAA | 3 cut(s) | 203, 240, 248 |
| Msp20I | TGGCCA | 1 cut(s) | 1091 |
| MspI | CCGG | 1 cut(s) | 1250 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 287, 487 |
| NcoI | CCATGG | 1 cut(s) | 393 |
| NdeI | CATATG | 3 cut(s) | 142, 186, 316 |
| NdeII | GATC | 2 cut(s) | 79, 1282 |
| NlaIII | CATG | 6 cut(s) | 397, 508, 691, 777, 1083, 1295 |
| NlaIV | GGNNCC | 2 cut(s) | 1224, 1302 |
| NmuCI | GTSAC | 1 cut(s) | 267 |
| NspI | RCATGY | 2 cut(s) | 691, 1083 |
| PagI | TCATGA | 1 cut(s) | 504 |
| PfeI | GAWTC | 5 cut(s) | 104, 779, 826, 967, 1216 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 899, 1297 |
| PkrI | GCNGC | 6 cut(s) | 138, 171, 345, 663, 720, 723 |
| PpuMI | RGGWCCY | 1 cut(s) | 1301 |
| PsiI | TTATAA | 1 cut(s) | 228 |
| Psp5II | RGGWCCY | 1 cut(s) | 1301 |
| PspN4I | GGNNCC | 2 cut(s) | 1224, 1302 |
| PspPI | GGNCC | 1 cut(s) | 1301 |
| PspPPI | RGGWCCY | 1 cut(s) | 1301 |
| RsaI | GTAC | 1 cut(s) | 952 |
| RsaNI | GTAC | 1 cut(s) | 951 |
| SaqAI | TTAA | 3 cut(s) | 203, 240, 248 |
| SatI | GCNGC | 6 cut(s) | 137, 170, 344, 662, 719, 722 |
| Sau3AI | GATC | 2 cut(s) | 79, 1282 |
| Sau96I | GGNCC | 1 cut(s) | 1301 |
| SduI | GDGCHC | 1 cut(s) | 28 |
| SfaNI | GCATC | 1 cut(s) | 1011 |
| SinI | GGWCC | 1 cut(s) | 1301 |
| SsiI | CCGC | 1 cut(s) | 719 |
| SspMI | CTAG | 4 cut(s) | 644, 813, 1232, 1305 |
| StyI | CCWWGG | 3 cut(s) | 282, 393, 1304 |
| TaiI | ACGT | 1 cut(s) | 765 |
| TaqI | TCGA | 1 cut(s) | 557 |
| TatI | WGTACW | 1 cut(s) | 950 |
| TauI | GCSGC | 1 cut(s) | 721 |
| TfiI | GAWTC | 5 cut(s) | 104, 779, 826, 967, 1216 |
| Tru1I | TTAA | 3 cut(s) | 203, 240, 248 |
| Tru9I | TTAA | 3 cut(s) | 203, 240, 248 |
| TseFI | GTSAC | 1 cut(s) | 267 |
| TseI | GCWGC | 5 cut(s) | 136, 169, 343, 661, 721 |
| Tsp45I | GTSAC | 1 cut(s) | 267 |
| TspDTI | ATGAA | 6 cut(s) | 173, 258, 493, 521, 762, 1229 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 899, 1297 |
| VpaK11BI | GGWCC | 1 cut(s) | 1301 |
| XapI | RAATTY | 9 cut(s) | 205, 234, 250, 408, 513, 932, 1046, 1177, 1235 |
| XceI | RCATGY | 2 cut(s) | 691, 1083 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 69 |
| XmaJI | CCTAGG | 1 cut(s) | 1304 |
| XspI | CTAG | 4 cut(s) | 644, 813, 1232, 1305 |
| ZraI | GACGTC | 1 cut(s) | 763 |