Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

tig00012681

Orthogroup ID

OG0000120

Physical Location & Seq

Genomic Coordinates Reverse (-)

82725 .. 84695

Gene Structure 1971 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12681g00060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 714 bp

ATGGCAAACTCATCGAACCTTAGCTTGGAATTGAGCCTTAGCAGTGATACGGGCGCGCCGCGTCAAGGTAACGTATGGCGCCCTTCTTTCTTATCTTCTAACGGTCTTCTTACAGGTGAAGACTCTGTGATGCAGGACGCCACAACAGCTACAATAGTAGCTAGGAACCTCCTCACTCCAAAAGATAGTAGGCTGCTGTCAGGACGGTCCGATGAGTTGGCCGTTCAAGAGTCCCTCGCACTTAGTGTTCAGTGTGCGAGTTCTGTGTCCAACATGGGCCAACGCCTGCTTGCCCGCTCCCGTCAGGTGGAATCATTGATGGCAGAGGTGGAAAGTCTTAAGCAAGAGATCCAGCAGCTTAAGTACGAGAATAGAAGTTTGCACGTGCTTGCCAACAACTATTCGACGGGCATGAAGAGGAAGCTTGATGAGCTGCAAGAGTCTGAAGGTCGGATTCACAGTGACCGTCAAAGGTTTTCGTCTTTCCTCCAGAGGCACCTTTTTCCTGGCTCATCCAGCGCTTGGCCAAGTATTGAGGCCTGGAATGCTCTAGCTCCGATGCCTCCCACTCCGATGCCTTCCACTCCGATGCCTTCCGCTTCGATGCCTTCCGCTCCGATGCCTTCTGCTTCAAAGCCTTCTGCTTCTATGCCTTCCGCTACTATTAAAGGGGGATTTAACATAAACATATATTTCGAATCACCCCCTAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008238 GO:0008239 GO:0016787 GO:0019538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NUDIX Peptidase_M49

Protein Analysis

238

Amino Acids

25.7

Weight (kDa)

6.85

Isoelectric Point (pI)

69.04

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000120)

Species	Orthologous Gene IDs
malus_domestica	MD00G1178700.v1.1 MD00G1192600.v1.1 MD01G1178800.v1.1 MD08G1118400.v1.1 MD11G1121800.v1.1 MD11G1175900.v1.1 MD14G1073500.v1.1 MD16G1171800.v1.1
prunus_persica	Prupe.5G057200_v2.0.a1 Prupe.7G095400_v2.0.a1 Prupe.7G095500_v2.0.a1 Prupe.8G037200_v2.0.a1
pyrus_communis	pycom01g00040 pycom01g00270 pycom01g00550 pycom01g00730 pycom01g00740 pycom01g00820 pycom01g00960 pycom01g01060 pycom01g01180 pycom01g01580 pycom01g01700 pycom01g01770 pycom01g01970 pycom01g02520 pycom01g03120 pycom01g03510 pycom01g07770 pycom01g07800 pycom01g11420 pycom02g06240 pycom02g13440 pycom02g16920 pycom02g17900 pycom02g18220 pycom02g21560 pycom03g10720 pycom03g11710 pycom03g11930 pycom04g05770 pycom04g07600 pycom04g07920 pycom04g07980 pycom04g08250 pycom04g09780 pycom04g12720 pycom04g13700 pycom05g00180 pycom05g01110 pycom05g05940 pycom05g06250 pycom05g06870 pycom05g08470 pycom05g08920 pycom05g09050 pycom05g09420 pycom05g14090 pycom06g05150 pycom06g05430 pycom06g06950 pycom06g09990 pycom06g15520 pycom07g08780 pycom07g11240 pycom07g11990 pycom07g14400 pycom07g27820 pycom08g07740 pycom08g10980 pycom08g12370 pycom09g03580 pycom09g09700 pycom09g13450 pycom09g14910 pycom10g01230 pycom10g02170 pycom10g06850 pycom10g08040 pycom10g08250 pycom10g10100 pycom10g15420 pycom1125g00010 pycom11g04190 pycom11g14150 pycom11g15090 pycom11g15720 pycom11g16230 pycom11g17180 pycom11g20110 pycom1236g00020 pycom12426g00290 pycom12426g00350 pycom12426g00660 pycom12426g00700 pycom12462g00070 pycom12463g00060 pycom12473g00010 pycom12503g00010 pycom12517g00150 pycom1256g00180 pycom1256g00220 pycom12575g00030 pycom12581g00010 pycom12596g00090 pycom12612g00020 pycom12621g00090 pycom12633g00040 pycom12637g00020 pycom12658g00010 pycom12658g00020 pycom12659g00070 pycom12668g00040 pycom12668g00050 pycom12674g00040 pycom12681g00050 pycom12681g00060 pycom12690g00020 pycom12695g00020 pycom12g08040 pycom12g08660 pycom12g09550 pycom12g09560 pycom13g03230 pycom13g18200 pycom13g19230 pycom13g20140 pycom13g20700 pycom13g22210 pycom13g23170 pycom13g25290 pycom13g25590 pycom13g25980 pycom13g26010 pycom13g26280 pycom13g26350 pycom13g27560 pycom13g27570 pycom13g27680 pycom13g27780 pycom13g28070 pycom13g28140 pycom13g28230 pycom13g28280 pycom13g29100 pycom1498g00010 pycom14g01730 pycom14g08250 pycom14g08660 pycom14g09260 pycom14g18640 pycom15g28640 pycom15g30060 pycom15g35030 pycom15g37780 pycom1604g00160 pycom1609g00100 pycom1681g00030 pycom1683g00020 pycom16g13500 pycom16g17920 pycom16g18140 pycom16g19340 pycom16g20200 pycom16g21370 pycom16g22140 pycom16g23170 pycom16g23450 pycom16g25040 pycom16g25790 pycom1729g00130 pycom1729g00180 pycom1739g00060 pycom1774g00010 pycom17g06230 pycom17g15230 pycom17g16620 pycom17g17340 pycom17g17660 pycom17g17810 pycom17g18150 pycom17g19280 pycom17g25050 pycom17g25100 pycom17g25680 pycom17g25690 pycom1880g00030 pycom1912g00020 pycom2002g00120 pycom2002g00160 pycom2177g00180 pycom2185g00070 pycom2189g00010 pycom2218g00030 pycom2320g00010 pycom2328g00040 pycom2339g00100 pycom2424g00030 pycom2466g00010 pycom2552g00010 pycom2599g00010 pycom2627g00040 pycom2749g00080 pycom2789g00010 pycom3027g00010 pycom394g00080 pycom436g00190 pycom436g00310 pycom436g00350 pycom436g00410 pycom436g00420 pycom436g00430 pycom443g00010 pycom505g00030 pycom520g00140 pycom520g00220 pycom520g00620 pycom520g00650 pycom555g00010 pycom57g00030 pycom608g00020 pycom723g00040 pycom775g00040 pycom808g00350 pycom808g00590 pycom808g00640 pycom889g00030 pycom908g00040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	78, 495
AccB7I	CCANNNNNTGG	1 cut(s)	522
AccBSI	CCGCTC	2 cut(s)	297, 614
AccII	CGCG	2 cut(s)	56, 61
AciI	CCGC	5 cut(s)	59, 295, 597, 612, 657
AclWI	GGATC	1 cut(s)	343
AcoI	YGGCCR	2 cut(s)	219, 524
AcuI	CTGAAG	1 cut(s)	465
AcvI	CACGTG	1 cut(s)	385
AcyI	GRCGYC	2 cut(s)	79, 138
AdeI	CACNNNGTG	1 cut(s)	245
AfaI	GTAC	1 cut(s)	365
AfeI	AGCGCT	1 cut(s)	520
AfiI	CCNNNNNNNGG	4 cut(s)	25, 65, 307, 522
AflII	CTTAAG	2 cut(s)	338, 359
AgsI	TTSAA	2 cut(s)	227, 633
AjnI	CCWGG	2 cut(s)	505, 539
AjuI	GAANNNNNNNTTGG	2 cut(s)	8, 40
AluBI	AGCT	8 cut(s)	24, 149, 161, 358, 424, 433, 554, 711
AluI	AGCT	8 cut(s)	24, 149, 161, 358, 424, 433, 554, 711
AlwI	GGATC	1 cut(s)	343
Aor51HI	AGCGCT	1 cut(s)	520
AoxI	GGCC	4 cut(s)	219, 277, 524, 537
ApeKI	GCWGC	3 cut(s)	193, 355, 433
AscI	GGCGCGCC	1 cut(s)	54
AspLEI	GCGC	4 cut(s)	56, 58, 81, 521
AspS9I	GGNCC	2 cut(s)	207, 277
AsuHPI	GGTGA	2 cut(s)	128, 693
AsuII	TTCGAA	1 cut(s)	696
AvaII	GGWCC	1 cut(s)	207
BalI	TGGCCA	1 cut(s)	526
BanI	GGYRCC	2 cut(s)	78, 495
BbrPI	CACGTG	1 cut(s)	385
BbsI	GAAGAC	2 cut(s)	98, 126
BbvI	GCAGC	3 cut(s)	180, 367, 420
BccI	CCATC	1 cut(s)	313
BceAI	ACGGC	1 cut(s)	206
BcgI	CGANNNNNNTGC	1 cut(s)	28
BciT130I	CCWGG	2 cut(s)	507, 541
BfaI	CTAG	3 cut(s)	162, 551, 708
BfoI	RGCGCY	2 cut(s)	82, 522
BfrI	CTTAAG	2 cut(s)	338, 359
BisI	GCNGC	4 cut(s)	59, 194, 356, 434
BlsI	GCNGC	4 cut(s)	60, 195, 357, 435
Bme1390I	CCNGG	2 cut(s)	507, 541
Bme18I	GGWCC	1 cut(s)	207
BmgT120I	GGNCC	2 cut(s)	207, 277
BmiI	GGNNCC	3 cut(s)	80, 167, 497
BmrFI	CCNGG	2 cut(s)	507, 541
BmsI	GCATC	6 cut(s)	120, 549, 564, 579, 594, 609
BpiI	GAAGAC	2 cut(s)	98, 126
BpmI	CTGGAG	1 cut(s)	473
Bpu10I	CCTNAGC	2 cut(s)	20, 38
Bpu14I	TTCGAA	1 cut(s)	696
BsaAI	YACGTR	1 cut(s)	385
BsaHI	GRCGYC	2 cut(s)	79, 138
Bsc4I	CCNNNNNNNGG	4 cut(s)	25, 65, 307, 522
BseBI	CCWGG	2 cut(s)	507, 541
BseGI	GGATG	1 cut(s)	512
BseLI	CCNNNNNNNGG	4 cut(s)	25, 65, 307, 522
BsePI	GCGCGC	1 cut(s)	54
BseRI	GAGGAG	1 cut(s)	161
BseXI	GCAGC	3 cut(s)	180, 367, 420
Bsh1236I	CGCG	2 cut(s)	56, 61
BshFI	GGCC	4 cut(s)	221, 279, 526, 539
BshNI	GGYRCC	2 cut(s)	78, 495
BslFI	GGGAC	1 cut(s)	217
BslI	CCNNNNNNNGG	4 cut(s)	25, 65, 307, 522
BsmFI	GGGAC	1 cut(s)	217
BsmI	GAATGC	1 cut(s)	550
BsnI	GGCC	4 cut(s)	221, 279, 526, 539
Bsp119I	TTCGAA	1 cut(s)	696
Bsp143I	GATC	1 cut(s)	348
BspACI	CCGC	5 cut(s)	59, 295, 597, 612, 657
BspANI	GGCC	4 cut(s)	221, 279, 526, 539
BspFNI	CGCG	2 cut(s)	56, 61
BspLI	GGNNCC	3 cut(s)	80, 167, 497
BspPI	GGATC	1 cut(s)	343
BspT104I	TTCGAA	1 cut(s)	696
BspT107I	GGYRCC	2 cut(s)	78, 495
BspTI	CTTAAG	2 cut(s)	338, 359
BsrBI	CCGCTC	2 cut(s)	297, 614
BssHII	GCGCGC	1 cut(s)	54
BssMI	GATC	1 cut(s)	348
BssNI	GRCGYC	2 cut(s)	79, 138
Bst2UI	CCWGG	2 cut(s)	507, 541
Bst4CI	ACNGT	4 cut(s)	104, 207, 461, 467
Bst6I	CTCTTC	1 cut(s)	410
BstACI	GRCGYC	2 cut(s)	79, 138
BstAFI	CTTAAG	2 cut(s)	338, 359
BstBAI	YACGTR	1 cut(s)	385
BstBI	TTCGAA	1 cut(s)	696
BstC8I	GCNNGC	5 cut(s)	56, 287, 291, 295, 390
BstDEI	CTNAG	3 cut(s)	20, 38, 242
BstF5I	GGATG	1 cut(s)	512
BstFNI	CGCG	2 cut(s)	56, 61
BstH2I	RGCGCY	2 cut(s)	82, 522
BstHHI	GCGC	4 cut(s)	56, 58, 81, 521
BstKTI	GATC	1 cut(s)	351
BstMBI	GATC	1 cut(s)	348
BstMWI	GCNNNNNNNGC	4 cut(s)	146, 430, 516, 545
BstNI	CCWGG	2 cut(s)	507, 541
BstSCI	CCNGG	2 cut(s)	505, 539
BstUI	CGCG	2 cut(s)	56, 61
BstV1I	GCAGC	3 cut(s)	180, 367, 420
BstV2I	GAAGAC	2 cut(s)	98, 126
BstX2I	RGATCY	1 cut(s)	348
BstYI	RGATCY	1 cut(s)	348
BsuRI	GGCC	4 cut(s)	221, 279, 526, 539
BtsCI	GGATG	1 cut(s)	512
BtsI	GCAGTG	1 cut(s)	49
BtsIMutI	CAGTG	3 cut(s)	49, 257, 466
Cac8I	GCNNGC	5 cut(s)	56, 287, 291, 295, 390
CfoI	GCGC	4 cut(s)	56, 58, 81, 521
Cfr13I	GGNCC	2 cut(s)	207, 277
CpoI	CGGWCCG	1 cut(s)	207
CseI	GACGC	2 cut(s)	50, 146
Csp6I	GTAC	1 cut(s)	364
CspI	CGGWCCG	1 cut(s)	207
CviAII	CATG	2 cut(s)	274, 412
CviQI	GTAC	1 cut(s)	364
DdeI	CTNAG	3 cut(s)	20, 38, 242
DinI	GGCGCC	1 cut(s)	80
DpnI	GATC	1 cut(s)	350
DpnII	GATC	1 cut(s)	348
DraIII	CACNNNGTG	1 cut(s)	245
EaeI	YGGCCR	2 cut(s)	219, 524
Eam1104I	CTCTTC	1 cut(s)	410
EarI	CTCTTC	1 cut(s)	410
Eco147I	AGGCCT	1 cut(s)	539
Eco47I	GGWCC	1 cut(s)	207
Eco47III	AGCGCT	1 cut(s)	520
Eco57I	CTGAAG	1 cut(s)	465
Eco72I	CACGTG	1 cut(s)	385
EcoRII	CCWGG	2 cut(s)	505, 539
EgeI	GGCGCC	1 cut(s)	80
EheI	GGCGCC	1 cut(s)	80
FaeI	CATG	2 cut(s)	277, 415
FaiI	YATR	7 cut(s)	76, 275, 413, 650, 683, 689, 691
FaqI	GGGAC	1 cut(s)	217
FatI	CATG	2 cut(s)	273, 411
FauI	CCCGC	1 cut(s)	302
Fnu4HI	GCNGC	4 cut(s)	59, 194, 356, 434
FokI	GGATG	1 cut(s)	499
Fsp4HI	GCNGC	4 cut(s)	59, 194, 356, 434
FspBI	CTAG	3 cut(s)	162, 551, 708
GlaI	GCGC	4 cut(s)	55, 57, 80, 520
GluI	GCNGC	4 cut(s)	59, 194, 356, 434
GsuI	CTGGAG	1 cut(s)	473
HaeII	RGCGCY	2 cut(s)	82, 522
HaeIII	GGCC	4 cut(s)	221, 279, 526, 539
HgaI	GACGC	2 cut(s)	50, 146
HhaI	GCGC	4 cut(s)	56, 58, 81, 521
Hin1I	GRCGYC	2 cut(s)	79, 138
Hin1II	CATG	2 cut(s)	277, 415
Hin6I	GCGC	4 cut(s)	54, 56, 79, 519
HinP1I	GCGC	4 cut(s)	54, 56, 79, 519
HindIII	AAGCTT	1 cut(s)	422
HinfI	GANTC	6 cut(s)	122, 230, 311, 440, 454, 698
HphI	GGTGA	2 cut(s)	128, 693
Hpy188I	TCNGA	7 cut(s)	211, 445, 453, 558, 573, 588, 618
Hpy188III	TCNNGA	3 cut(s)	201, 227, 490
Hpy99I	CGWCG	1 cut(s)	409
HpyAV	CCTTC	8 cut(s)	93, 440, 588, 603, 618, 633, 648, 663
HpyCH4III	ACNGT	4 cut(s)	104, 207, 461, 467
HpyCH4IV	ACGT	2 cut(s)	72, 384
HpyCH4V	TGCA	3 cut(s)	133, 382, 436
HpyF10VI	GCNNNNNNNGC	4 cut(s)	146, 430, 516, 545
HpyF3I	CTNAG	3 cut(s)	20, 38, 242
HpySE526I	ACGT	2 cut(s)	72, 384
Hsp92I	GRCGYC	2 cut(s)	79, 138
Hsp92II	CATG	2 cut(s)	277, 415
HspAI	GCGC	4 cut(s)	54, 56, 79, 519
KasI	GGCGCC	1 cut(s)	78
Kzo9I	GATC	1 cut(s)	348
LmnI	GCTCC	3 cut(s)	302, 559, 619
Lsp1109I	GCAGC	3 cut(s)	180, 367, 420
LweI	GCATC	6 cut(s)	120, 549, 564, 579, 594, 609
MaeI	CTAG	3 cut(s)	162, 551, 708
MaeII	ACGT	2 cut(s)	72, 384
MaeIII	GTNAC	2 cut(s)	68, 461
MalI	GATC	1 cut(s)	350
MbiI	CCGCTC	2 cut(s)	297, 614
MboI	GATC	1 cut(s)	348
MboII	GAAGA	4 cut(s)	87, 98, 131, 427
MflI	RGATCY	1 cut(s)	348
MlsI	TGGCCA	1 cut(s)	526
MluCI	AATT	1 cut(s)	29
MluNI	TGGCCA	1 cut(s)	526
Mly113I	GGCGCC	1 cut(s)	79
MlyI	GAGTC	3 cut(s)	116, 239, 449
MmeI	TCCRAC	2 cut(s)	294, 431
MnlI	CCTC	9 cut(s)	179, 182, 245, 319, 411, 486, 497, 529, 573
Mox20I	TGGCCA	1 cut(s)	526
MscI	TGGCCA	1 cut(s)	526
MseI	TTAA	4 cut(s)	339, 360, 666, 678
MslI	CAYNNNNRTG	2 cut(s)	572, 587
Msp20I	TGGCCA	1 cut(s)	526
MspCI	CTTAAG	2 cut(s)	338, 359
MspR9I	CCNGG	2 cut(s)	507, 541
Mva1269I	GAATGC	1 cut(s)	550
MvaI	CCWGG	2 cut(s)	507, 541
MvnI	CGCG	2 cut(s)	56, 61
MwoI	GCNNNNNNNGC	4 cut(s)	146, 430, 516, 545
NarI	GGCGCC	1 cut(s)	79
NdeII	GATC	1 cut(s)	348
NlaIII	CATG	2 cut(s)	277, 415
NlaIV	GGNNCC	3 cut(s)	80, 167, 497
NmuCI	GTSAC	1 cut(s)	461
NspV	TTCGAA	1 cut(s)	696
PalAI	GGCGCGCC	1 cut(s)	54
PauI	GCGCGC	1 cut(s)	54
PceI	AGGCCT	1 cut(s)	539
PctI	GAATGC	1 cut(s)	550
PfeI	GAWTC	3 cut(s)	311, 454, 698
PflMI	CCANNNNNTGG	1 cut(s)	522
PkrI	GCNGC	4 cut(s)	60, 195, 357, 435
PleI	GAGTC	3 cut(s)	116, 238, 448
PluTI	GGCGCC	1 cut(s)	82
PmaCI	CACGTG	1 cut(s)	385
PmlI	CACGTG	1 cut(s)	385
PpsI	GAGTC	3 cut(s)	116, 238, 448
Ppu21I	YACGTR	1 cut(s)	385
Psp6I	CCWGG	2 cut(s)	505, 539
PspCI	CACGTG	1 cut(s)	385
PspGI	CCWGG	2 cut(s)	505, 539
PspN4I	GGNNCC	3 cut(s)	80, 167, 497
PspPI	GGNCC	2 cut(s)	207, 277
PsuI	RGATCY	1 cut(s)	348
PteI	GCGCGC	1 cut(s)	54
RsaI	GTAC	1 cut(s)	365
RsaNI	GTAC	1 cut(s)	364
RseI	CAYNNNNRTG	2 cut(s)	572, 587
Rsr2I	CGGWCCG	1 cut(s)	207
RsrII	CGGWCCG	1 cut(s)	207
SaqAI	TTAA	4 cut(s)	339, 360, 666, 678
SatI	GCNGC	4 cut(s)	59, 194, 356, 434
Sau3AI	GATC	1 cut(s)	348
Sau96I	GGNCC	2 cut(s)	207, 277
SchI	GAGTC	3 cut(s)	116, 239, 449
ScrFI	CCNGG	2 cut(s)	507, 541
SfaNI	GCATC	6 cut(s)	120, 549, 564, 579, 594, 609
SfoI	GGCGCC	1 cut(s)	80
SfuI	TTCGAA	1 cut(s)	696
SgsI	GGCGCGCC	1 cut(s)	54
SinI	GGWCC	1 cut(s)	207
SmiMI	CAYNNNNRTG	2 cut(s)	572, 587
SmlI	CTYRAG	2 cut(s)	338, 359
SmoI	CTYRAG	2 cut(s)	338, 359
Sse9I	AATT	1 cut(s)	29
SseBI	AGGCCT	1 cut(s)	539
SsiI	CCGC	5 cut(s)	59, 295, 597, 612, 657
SspDI	GGCGCC	1 cut(s)	78
SspMI	CTAG	3 cut(s)	162, 551, 708
StuI	AGGCCT	1 cut(s)	539
StyD4I	CCNGG	2 cut(s)	505, 539
TaaI	ACNGT	4 cut(s)	104, 207, 461, 467
TaiI	ACGT	2 cut(s)	75, 387
TaqI	TCGA	4 cut(s)	14, 404, 602, 696
TasI	AATT	1 cut(s)	29
TauI	GCSGC	1 cut(s)	61
TfiI	GAWTC	3 cut(s)	311, 454, 698
Tru1I	TTAA	4 cut(s)	339, 360, 666, 678
Tru9I	TTAA	4 cut(s)	339, 360, 666, 678
TscAI	CASTG	3 cut(s)	49, 257, 466
TseFI	GTSAC	1 cut(s)	461
TseI	GCWGC	3 cut(s)	193, 355, 433
Tsp45I	GTSAC	1 cut(s)	461
TspDTI	ATGAA	1 cut(s)	428
TspRI	CASTG	3 cut(s)	49, 257, 466
Van91I	CCANNNNNTGG	1 cut(s)	522
Vha464I	CTTAAG	2 cut(s)	338, 359
VpaK11BI	GGWCC	1 cut(s)	207
XspI	CTAG	3 cut(s)	162, 551, 708

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.