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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 826 |
| AccB1I | GGYRCC | 4 cut(s) | 477, 1170, 1516, 2412 |
| AccBSI | CCGCTC | 4 cut(s) | 1643, 1658, 1688, 1865 |
| AccI | GTMKAC | 2 cut(s) | 2489, 2540 |
| AccII | CGCG | 4 cut(s) | 452, 2208, 2279, 2381 |
| AciI | CCGC | 8 cut(s) | 968, 1643, 1658, 1688, 1724, 1865, 2240, 2592 |
| AclWI | GGATC | 1 cut(s) | 393 |
| AcoI | YGGCCR | 1 cut(s) | 1383 |
| AcuI | CTGAAG | 2 cut(s) | 2138, 2591 |
| AcvI | CACGTG | 1 cut(s) | 2303 |
| AcyI | GRCGYC | 4 cut(s) | 478, 1976, 2413, 2436 |
| AdeI | CACNNNGTG | 1 cut(s) | 1920 |
| AfaI | GTAC | 2 cut(s) | 2510, 2677 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 487, 1140, 1145, 1267, 1631, 2006 |
| AflIII | ACRYGT | 5 cut(s) | 681, 2206, 2277, 2379, 2735 |
| AjnI | CCWGG | 2 cut(s) | 2669, 2835 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 939, 971 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2119, 2151 |
| AluBI | AGCT | 8 cut(s) | 622, 1597, 1784, 1793, 1955, 1967, 2047, 2322 |
| AluI | AGCT | 8 cut(s) | 622, 1597, 1784, 1793, 1955, 1967, 2047, 2322 |
| Alw21I | GWGCWC | 2 cut(s) | 172, 303 |
| Alw26I | GTCTC | 4 cut(s) | 559, 2122, 2473, 2711 |
| AlwI | GGATC | 1 cut(s) | 393 |
| Ama87I | CYCGRG | 1 cut(s) | 2873 |
| AoxI | GGCC | 4 cut(s) | 1383, 1707, 1881, 2122 |
| ApeKI | GCWGC | 5 cut(s) | 772, 805, 1781, 2319, 2741 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1727, 1739, 1759, 1771 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2844 |
| AspLEI | GCGC | 4 cut(s) | 452, 480, 2415, 2608 |
| AspS9I | GGNCC | 6 cut(s) | 633, 1090, 1576, 1882, 2009, 2122 |
| AsuC2I | CCSGG | 1 cut(s) | 488 |
| AsuHPI | GGTGA | 6 cut(s) | 230, 1262, 1282, 1553, 1844, 1989 |
| AvaI | CYCGRG | 1 cut(s) | 2873 |
| AvaII | GGWCC | 4 cut(s) | 633, 1090, 1576, 2009 |
| BalI | TGGCCA | 1 cut(s) | 1385 |
| BanI | GGYRCC | 4 cut(s) | 477, 1170, 1516, 2412 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1611, 1643 |
| BbrPI | CACGTG | 1 cut(s) | 2303 |
| BbsI | GAAGAC | 2 cut(s) | 1986, 2806 |
| Bbv12I | GWGCWC | 2 cut(s) | 172, 303 |
| BbvI | GCAGC | 5 cut(s) | 784, 817, 1793, 2331, 2728 |
| BccI | CCATC | 7 cut(s) | 49, 1108, 1197, 1376, 2051, 2092, 2096 |
| BceAI | ACGGC | 4 cut(s) | 1157, 1574, 2443, 2738 |
| BciT130I | CCWGG | 2 cut(s) | 2671, 2837 |
| BciVI | GTATCC | 1 cut(s) | 1217 |
| BclI | TGATCA | 1 cut(s) | 715 |
| BcnI | CCSGG | 1 cut(s) | 488 |
| BcoDI | GTCTC | 4 cut(s) | 559, 2122, 2473, 2711 |
| BfaI | CTAG | 4 cut(s) | 1008, 1526, 1952, 2862 |
| BfoI | RGCGCY | 3 cut(s) | 481, 2416, 2609 |
| BfuAI | ACCTGC | 1 cut(s) | 826 |
| BfuI | GTATCC | 1 cut(s) | 1217 |
| BisI | GCNGC | 6 cut(s) | 773, 806, 1724, 1782, 2320, 2742 |
| BlsI | GCNGC | 6 cut(s) | 774, 807, 1725, 1783, 2321, 2743 |
| Bme1390I | CCNGG | 3 cut(s) | 488, 2671, 2837 |
| Bme18I | GGWCC | 4 cut(s) | 633, 1090, 1576, 2009 |
| BmeT110I | CYCGRG | 1 cut(s) | 2873 |
| BmgT120I | GGNCC | 6 cut(s) | 633, 1090, 1576, 1882, 2009, 2122 |
| BmiI | GGNNCC | 9 cut(s) | 479, 635, 741, 1092, 1172, 1518, 1949, 2414, 2827 |
| BmrFI | CCNGG | 3 cut(s) | 488, 2671, 2837 |
| BmsI | GCATC | 7 cut(s) | 474, 1644, 1659, 1674, 1689, 1704, 1992 |
| BoxI | GACNNNNGTC | 2 cut(s) | 2485, 2546 |
| BpiI | GAAGAC | 2 cut(s) | 1986, 2806 |
| BpmI | CTGGAG | 1 cut(s) | 2692 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2048 |
| BpuMI | CCSGG | 1 cut(s) | 488 |
| Bsa29I | ATCGAT | 1 cut(s) | 2179 |
| BsaAI | YACGTR | 2 cut(s) | 1616, 2303 |
| BsaHI | GRCGYC | 4 cut(s) | 478, 1976, 2413, 2436 |
| BsaJI | CCNNGG | 6 cut(s) | 487, 994, 1050, 1139, 1236, 2198 |
| BsaWI | WCCGGW | 1 cut(s) | 1164 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2119, 2149 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 487, 1140, 1145, 1267, 1631, 2006 |
| Bse118I | RCCGGY | 1 cut(s) | 1543 |
| Bse3DI | GCAATG | 3 cut(s) | 481, 835, 1017 |
| BseBI | CCWGG | 2 cut(s) | 2671, 2837 |
| BseCI | ATCGAT | 1 cut(s) | 2179 |
| BseDI | CCNNGG | 6 cut(s) | 487, 994, 1050, 1139, 1236, 2198 |
| BseGI | GGATG | 2 cut(s) | 2103, 2107 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 487, 1140, 1145, 1267, 1631, 2006 |
| BseMI | GCAATG | 3 cut(s) | 481, 835, 1017 |
| BseMII | CTCAG | 2 cut(s) | 2480, 2591 |
| BseRI | GAGGAG | 1 cut(s) | 1957 |
| BseXI | GCAGC | 5 cut(s) | 784, 817, 1793, 2331, 2728 |
| BseYI | CCCAGC | 1 cut(s) | 2613 |
| BsgI | GTGCAG | 1 cut(s) | 2440 |
| Bsh1236I | CGCG | 4 cut(s) | 452, 2208, 2279, 2381 |
| Bsh1285I | CGRYCG | 2 cut(s) | 2701, 2705 |
| BshFI | GGCC | 4 cut(s) | 1385, 1709, 1883, 2124 |
| BshNI | GGYRCC | 4 cut(s) | 477, 1170, 1516, 2412 |
| BshVI | ATCGAT | 1 cut(s) | 2179 |
| BsiEI | CGRYCG | 2 cut(s) | 2701, 2705 |
| BsiHKAI | GWGCWC | 2 cut(s) | 172, 303 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2873 |
| BsiSI | CCGG | 6 cut(s) | 487, 1165, 1544, 2125, 2228, 2237 |
| BslFI | GGGAC | 3 cut(s) | 619, 903, 1941 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 487, 1140, 1145, 1267, 1631, 2006 |
| BsmAI | GTCTC | 4 cut(s) | 559, 2122, 2473, 2711 |
| BsmBI | CGTCTC | 3 cut(s) | 559, 2122, 2473 |
| BsmFI | GGGAC | 3 cut(s) | 619, 903, 1941 |
| BsmI | GAATGC | 2 cut(s) | 353, 1760 |
| BsnI | GGCC | 4 cut(s) | 1385, 1709, 1883, 2124 |
| BsoBI | CYCGRG | 1 cut(s) | 2873 |
| Bsp1286I | GDGCHC | 2 cut(s) | 172, 303 |
| Bsp143I | GATC | 6 cut(s) | 398, 661, 715, 2176, 2448, 2702 |
| BspACI | CCGC | 8 cut(s) | 968, 1643, 1658, 1688, 1724, 1865, 2240, 2592 |
| BspANI | GGCC | 4 cut(s) | 1385, 1709, 1883, 2124 |
| BspCNI | CTCAG | 2 cut(s) | 2479, 2592 |
| BspDI | ATCGAT | 1 cut(s) | 2179 |
| BspFNI | CGCG | 4 cut(s) | 452, 2208, 2279, 2381 |
| BspHI | TCATGA | 2 cut(s) | 1227, 1288 |
| BspLI | GGNNCC | 9 cut(s) | 479, 635, 741, 1092, 1172, 1518, 1949, 2414, 2827 |
| BspMI | ACCTGC | 1 cut(s) | 826 |
| BspPI | GGATC | 1 cut(s) | 393 |
| BspQI | GCTCTTC | 1 cut(s) | 2448 |
| BspT107I | GGYRCC | 4 cut(s) | 477, 1170, 1516, 2412 |
| BsrBI | CCGCTC | 4 cut(s) | 1643, 1658, 1688, 1865 |
| BsrDI | GCAATG | 3 cut(s) | 481, 835, 1017 |
| BsrFI | RCCGGY | 1 cut(s) | 1543 |
| BssAI | RCCGGY | 1 cut(s) | 1543 |
| BssECI | CCNNGG | 6 cut(s) | 487, 994, 1050, 1139, 1236, 2198 |
| BssMI | GATC | 6 cut(s) | 398, 661, 715, 2176, 2448, 2702 |
| BssNI | GRCGYC | 4 cut(s) | 478, 1976, 2413, 2436 |
| BssT1I | CCWWGG | 4 cut(s) | 994, 1050, 1236, 2198 |
| Bst2UI | CCWGG | 2 cut(s) | 2671, 2837 |
| Bst4CI | ACNGT | 5 cut(s) | 1739, 1751, 2013, 2508, 2699 |
| Bst6I | CTCTTC | 7 cut(s) | 1115, 1698, 1804, 1854, 2448, 2471, 2593 |
| BstACI | GRCGYC | 4 cut(s) | 478, 1976, 2413, 2436 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1177 |
| BstBAI | YACGTR | 2 cut(s) | 1616, 2303 |
| BstC8I | GCNNGC | 6 cut(s) | 1728, 1827, 1867, 1871, 1875, 2383 |
| BstDEI | CTNAG | 5 cut(s) | 1917, 2043, 2048, 2466, 2600 |
| BstDSI | CCRYGG | 1 cut(s) | 1139 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1265, 2004 |
| BstF5I | GGATG | 2 cut(s) | 2103, 2107 |
| BstFNI | CGCG | 4 cut(s) | 452, 2208, 2279, 2381 |
| BstH2I | RGCGCY | 3 cut(s) | 481, 2416, 2609 |
| BstHHI | GCGC | 4 cut(s) | 452, 480, 2415, 2608 |
| BstKTI | GATC | 6 cut(s) | 401, 664, 718, 2179, 2451, 2705 |
| BstMAI | GTCTC | 4 cut(s) | 559, 2122, 2473, 2711 |
| BstMBI | GATC | 6 cut(s) | 398, 661, 715, 2176, 2448, 2702 |
| BstMCI | CGRYCG | 2 cut(s) | 2701, 2705 |
| BstNI | CCWGG | 2 cut(s) | 2671, 2837 |
| BstNSI | RCATGY | 6 cut(s) | 335, 685, 1377, 1835, 2714, 2739 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 2485, 2546 |
| BstSCI | CCNGG | 3 cut(s) | 486, 2669, 2835 |
| BstUI | CGCG | 4 cut(s) | 452, 2208, 2279, 2381 |
| BstV1I | GCAGC | 5 cut(s) | 784, 817, 1793, 2331, 2728 |
| BstV2I | GAAGAC | 2 cut(s) | 1986, 2806 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 2670, 2836 |
| Bsu15I | ATCGAT | 1 cut(s) | 2179 |
| BsuI | GTATCC | 1 cut(s) | 1217 |
| BsuRI | GGCC | 4 cut(s) | 1385, 1709, 1883, 2124 |
| BsuTUI | ATCGAT | 1 cut(s) | 2179 |
| BtgI | CCRYGG | 1 cut(s) | 1139 |
| BtgZI | GCGATG | 2 cut(s) | 2187, 2300 |
| BtsCI | GGATG | 2 cut(s) | 2103, 2107 |
| BtsIMutI | CAGTG | 1 cut(s) | 1907 |
| BveI | ACCTGC | 1 cut(s) | 826 |
| Cac8I | GCNNGC | 6 cut(s) | 1728, 1827, 1867, 1871, 1875, 2383 |
| CciI | TCATGA | 2 cut(s) | 1227, 1288 |
| CfoI | GCGC | 4 cut(s) | 452, 480, 2415, 2608 |
| Cfr10I | RCCGGY | 1 cut(s) | 1543 |
| Cfr13I | GGNCC | 6 cut(s) | 633, 1090, 1576, 1882, 2009, 2122 |
| ClaI | ATCGAT | 1 cut(s) | 2179 |
| CseI | GACGC | 7 cut(s) | 892, 1965, 2197, 2214, 2262, 2364, 2425 |
| Csp6I | GTAC | 2 cut(s) | 2509, 2676 |
| CviQI | GTAC | 2 cut(s) | 2509, 2676 |
| DdeI | CTNAG | 5 cut(s) | 1917, 2043, 2048, 2466, 2600 |
| DinI | GGCGCC | 2 cut(s) | 479, 2414 |
| DpnI | GATC | 6 cut(s) | 400, 663, 717, 2178, 2450, 2704 |
| DpnII | GATC | 6 cut(s) | 398, 661, 715, 2176, 2448, 2702 |
| DraI | TTTAAA | 1 cut(s) | 126 |
| DraIII | CACNNNGTG | 1 cut(s) | 1920 |
| EaeI | YGGCCR | 1 cut(s) | 1383 |
| Eam1104I | CTCTTC | 7 cut(s) | 1115, 1698, 1804, 1854, 2448, 2471, 2593 |
| EarI | CTCTTC | 7 cut(s) | 1115, 1698, 1804, 1854, 2448, 2471, 2593 |
| EciI | GGCGGA | 1 cut(s) | 2255 |
| Eco130I | CCWWGG | 4 cut(s) | 994, 1050, 1236, 2198 |
| Eco147I | AGGCCT | 1 cut(s) | 1709 |
| Eco47I | GGWCC | 4 cut(s) | 633, 1090, 1576, 2009 |
| Eco57I | CTGAAG | 2 cut(s) | 2138, 2591 |
| Eco72I | CACGTG | 1 cut(s) | 2303 |
| Eco88I | CYCGRG | 1 cut(s) | 2873 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1265, 2004 |
| EcoO109I | RGGNCCY | 1 cut(s) | 633 |
| EcoRI | GAATTC | 3 cut(s) | 785, 1292, 1529 |
| EcoRII | CCWGG | 2 cut(s) | 2669, 2835 |
| EcoT14I | CCWWGG | 4 cut(s) | 994, 1050, 1236, 2198 |
| EgeI | GGCGCC | 2 cut(s) | 479, 2414 |
| EheI | GGCGCC | 2 cut(s) | 479, 2414 |
| ErhI | CCWWGG | 4 cut(s) | 994, 1050, 1236, 2198 |
| Esp3I | CGTCTC | 3 cut(s) | 559, 2122, 2473 |
| FaqI | GGGAC | 3 cut(s) | 619, 903, 1941 |
| FauI | CCCGC | 2 cut(s) | 1681, 1858 |
| FauNDI | CATATG | 1 cut(s) | 778 |
| FbaI | TGATCA | 1 cut(s) | 715 |
| FblI | GTMKAC | 2 cut(s) | 2489, 2540 |
| Fnu4HI | GCNGC | 6 cut(s) | 773, 806, 1724, 1782, 2320, 2742 |
| FokI | GGATG | 2 cut(s) | 2110, 2114 |
| Fsp4HI | GCNGC | 6 cut(s) | 773, 806, 1724, 1782, 2320, 2742 |
| FspBI | CTAG | 4 cut(s) | 1008, 1526, 1952, 2862 |
| GlaI | GCGC | 4 cut(s) | 451, 479, 2414, 2607 |
| GluI | GCNGC | 6 cut(s) | 773, 806, 1724, 1782, 2320, 2742 |
| GsaI | CCCAGC | 1 cut(s) | 2617 |
| GsuI | CTGGAG | 1 cut(s) | 2692 |
| HaeII | RGCGCY | 3 cut(s) | 481, 2416, 2609 |
| HaeIII | GGCC | 4 cut(s) | 1385, 1709, 1883, 2124 |
| HapII | CCGG | 6 cut(s) | 487, 1165, 1544, 2125, 2228, 2237 |
| HgaI | GACGC | 7 cut(s) | 892, 1965, 2197, 2214, 2262, 2364, 2425 |
| HhaI | GCGC | 4 cut(s) | 452, 480, 2415, 2608 |
| Hin1I | GRCGYC | 4 cut(s) | 478, 1976, 2413, 2436 |
| Hin6I | GCGC | 4 cut(s) | 450, 478, 2413, 2606 |
| HinP1I | GCGC | 4 cut(s) | 450, 478, 2413, 2606 |
| HincII | GTYRAC | 2 cut(s) | 2490, 2541 |
| HindII | GTYRAC | 2 cut(s) | 2490, 2541 |
| HindIII | AAGCTT | 1 cut(s) | 1791 |
| HpaII | CCGG | 6 cut(s) | 487, 1165, 1544, 2125, 2228, 2237 |
| HphI | GGTGA | 6 cut(s) | 230, 1262, 1282, 1553, 1844, 1989 |
| Hpy166II | GTNNAC | 7 cut(s) | 1549, 1613, 2212, 2300, 2490, 2541, 2782 |
| Hpy8I | GTNNAC | 7 cut(s) | 1549, 1613, 2212, 2300, 2490, 2541, 2782 |
| Hpy99I | CGWCG | 3 cut(s) | 2208, 2256, 2358 |
| HpyCH4III | ACNGT | 5 cut(s) | 1739, 1751, 2013, 2508, 2699 |
| HpyCH4IV | ACGT | 5 cut(s) | 1308, 1615, 1938, 2302, 2482 |
| HpyF3I | CTNAG | 5 cut(s) | 1917, 2043, 2048, 2466, 2600 |
| HpySE526I | ACGT | 5 cut(s) | 1308, 1615, 1938, 2302, 2482 |
| Hsp92I | GRCGYC | 4 cut(s) | 478, 1976, 2413, 2436 |
| HspAI | GCGC | 4 cut(s) | 450, 478, 2413, 2606 |
| KasI | GGCGCC | 2 cut(s) | 477, 2412 |
| Ksp22I | TGATCA | 1 cut(s) | 715 |
| Kzo9I | GATC | 6 cut(s) | 398, 661, 715, 2176, 2448, 2702 |
| LguI | GCTCTTC | 1 cut(s) | 2448 |
| LmnI | GCTCC | 6 cut(s) | 1185, 1640, 1655, 1670, 1685, 1862 |
| Lsp1109I | GCAGC | 5 cut(s) | 784, 817, 1793, 2331, 2728 |
| LweI | GCATC | 7 cut(s) | 474, 1644, 1659, 1674, 1689, 1704, 1992 |
| MaeI | CTAG | 4 cut(s) | 1008, 1526, 1952, 2862 |
| MaeII | ACGT | 5 cut(s) | 1308, 1615, 1938, 2302, 2482 |
| MaeIII | GTNAC | 4 cut(s) | 32, 615, 1739, 1751 |
| MalI | GATC | 6 cut(s) | 400, 663, 717, 2178, 2450, 2704 |
| MbiI | CCGCTC | 4 cut(s) | 1643, 1658, 1688, 1865 |
| MboI | GATC | 6 cut(s) | 398, 661, 715, 2176, 2448, 2702 |
| MfeI | CAATTG | 2 cut(s) | 5, 422 |
| MhlI | GDGCHC | 2 cut(s) | 172, 303 |
| MlsI | TGGCCA | 1 cut(s) | 1385 |
| MluI | ACGCGT | 3 cut(s) | 2206, 2277, 2379 |
| MluNI | TGGCCA | 1 cut(s) | 1385 |
| Mly113I | GGCGCC | 2 cut(s) | 478, 2413 |
| MlyI | GAGTC | 5 cut(s) | 371, 885, 1923, 2028, 2816 |
| MmeI | TCCRAC | 6 cut(s) | 915, 919, 1063, 1422, 1870, 2607 |
| Mox20I | TGGCCA | 1 cut(s) | 1385 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2844 |
| MscI | TGGCCA | 1 cut(s) | 1385 |
| MseI | TTAA | 9 cut(s) | 93, 125, 195, 249, 326, 338, 428, 441, 2109 |
| MslI | CAYNNNNRTG | 2 cut(s) | 165, 296 |
| Msp20I | TGGCCA | 1 cut(s) | 1385 |
| MspI | CCGG | 6 cut(s) | 487, 1165, 1544, 2125, 2228, 2237 |
| MspR9I | CCNGG | 3 cut(s) | 488, 2671, 2837 |
| MunI | CAATTG | 2 cut(s) | 5, 422 |
| Mva1269I | GAATGC | 2 cut(s) | 353, 1760 |
| MvaI | CCWGG | 2 cut(s) | 2671, 2837 |
| MvnI | CGCG | 4 cut(s) | 452, 2208, 2279, 2381 |
| NarI | GGCGCC | 2 cut(s) | 478, 2413 |
| NciI | CCSGG | 1 cut(s) | 488 |
| NdeI | CATATG | 1 cut(s) | 778 |
| NdeII | GATC | 6 cut(s) | 398, 661, 715, 2176, 2448, 2702 |
| NlaIV | GGNNCC | 9 cut(s) | 479, 635, 741, 1092, 1172, 1518, 1949, 2414, 2827 |
| NmuCI | GTSAC | 3 cut(s) | 615, 1739, 1751 |
| NspI | RCATGY | 6 cut(s) | 335, 685, 1377, 1835, 2714, 2739 |
| PaeR7I | CTCGAG | 1 cut(s) | 2873 |
| PagI | TCATGA | 2 cut(s) | 1227, 1288 |
| PceI | AGGCCT | 1 cut(s) | 1709 |
| PciI | ACATGT | 2 cut(s) | 681, 2735 |
| PciSI | GCTCTTC | 1 cut(s) | 2448 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2212 |
| PctI | GAATGC | 2 cut(s) | 353, 1760 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2844 |
| PfeI | GAWTC | 6 cut(s) | 260, 1224, 1510, 1774, 2217, 2840 |
| PflFI | GACNNNGTC | 1 cut(s) | 2390 |
| PkrI | GCNGC | 6 cut(s) | 774, 807, 1725, 1783, 2321, 2743 |
| Ple19I | CGATCG | 1 cut(s) | 2705 |
| PleI | GAGTC | 5 cut(s) | 370, 885, 1923, 2028, 2815 |
| PluTI | GGCGCC | 2 cut(s) | 481, 2416 |
| PmaCI | CACGTG | 1 cut(s) | 2303 |
| PmlI | CACGTG | 1 cut(s) | 2303 |
| PpsI | GAGTC | 5 cut(s) | 370, 885, 1923, 2028, 2815 |
| Ppu21I | YACGTR | 2 cut(s) | 1616, 2303 |
| PpuMI | RGGWCCY | 1 cut(s) | 633 |
| PscI | ACATGT | 2 cut(s) | 681, 2735 |
| PshAI | GACNNNNGTC | 2 cut(s) | 2485, 2546 |
| Psp5II | RGGWCCY | 1 cut(s) | 633 |
| Psp6I | CCWGG | 2 cut(s) | 2669, 2835 |
| PspCI | CACGTG | 1 cut(s) | 2303 |
| PspFI | CCCAGC | 1 cut(s) | 2613 |
| PspGI | CCWGG | 2 cut(s) | 2669, 2835 |
| PspN4I | GGNNCC | 9 cut(s) | 479, 635, 741, 1092, 1172, 1518, 1949, 2414, 2827 |
| PspPI | GGNCC | 6 cut(s) | 633, 1090, 1576, 1882, 2009, 2122 |
| PspPPI | RGGWCCY | 1 cut(s) | 633 |
| PsyI | GACNNNGTC | 1 cut(s) | 2390 |
| PvuI | CGATCG | 1 cut(s) | 2705 |
| RsaI | GTAC | 2 cut(s) | 2510, 2677 |
| RsaNI | GTAC | 2 cut(s) | 2509, 2676 |
| RseI | CAYNNNNRTG | 2 cut(s) | 165, 296 |
| SalI | GTCGAC | 2 cut(s) | 2488, 2539 |
| SapI | GCTCTTC | 1 cut(s) | 2448 |
| SaqAI | TTAA | 9 cut(s) | 93, 125, 195, 249, 326, 338, 428, 441, 2109 |
| SatI | GCNGC | 6 cut(s) | 773, 806, 1724, 1782, 2320, 2742 |
| Sau3AI | GATC | 6 cut(s) | 398, 661, 715, 2176, 2448, 2702 |
| Sau96I | GGNCC | 6 cut(s) | 633, 1090, 1576, 1882, 2009, 2122 |
| SchI | GAGTC | 5 cut(s) | 371, 885, 1923, 2028, 2816 |
| ScrFI | CCNGG | 3 cut(s) | 488, 2671, 2837 |
| SduI | GDGCHC | 2 cut(s) | 172, 303 |
| SfaNI | GCATC | 7 cut(s) | 474, 1644, 1659, 1674, 1689, 1704, 1992 |
| SfoI | GGCGCC | 2 cut(s) | 479, 2414 |
| Sfr274I | CTCGAG | 1 cut(s) | 2873 |
| SinI | GGWCC | 4 cut(s) | 633, 1090, 1576, 2009 |
| SlaI | CTCGAG | 1 cut(s) | 2873 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 165, 296 |
| SmlI | CTYRAG | 1 cut(s) | 2873 |
| SmoI | CTYRAG | 1 cut(s) | 2873 |
| SseBI | AGGCCT | 1 cut(s) | 1709 |
| SsiI | CCGC | 8 cut(s) | 968, 1643, 1658, 1688, 1724, 1865, 2240, 2592 |
| SspDI | GGCGCC | 2 cut(s) | 477, 2412 |
| SspMI | CTAG | 4 cut(s) | 1008, 1526, 1952, 2862 |
| StuI | AGGCCT | 1 cut(s) | 1709 |
| StyD4I | CCNGG | 3 cut(s) | 486, 2669, 2835 |
| StyI | CCWWGG | 4 cut(s) | 994, 1050, 1236, 2198 |
| TaaI | ACNGT | 5 cut(s) | 1739, 1751, 2013, 2508, 2699 |
| TaiI | ACGT | 5 cut(s) | 1311, 1618, 1941, 2305, 2485 |
| TatI | WGTACW | 1 cut(s) | 2675 |
| TauI | GCSGC | 1 cut(s) | 1726 |
| TfiI | GAWTC | 6 cut(s) | 260, 1224, 1510, 1774, 2217, 2840 |
| Tru1I | TTAA | 9 cut(s) | 93, 125, 195, 249, 326, 338, 428, 441, 2109 |
| Tru9I | TTAA | 9 cut(s) | 93, 125, 195, 249, 326, 338, 428, 441, 2109 |
| TscAI | CASTG | 1 cut(s) | 1914 |
| TseFI | GTSAC | 3 cut(s) | 615, 1739, 1751 |
| TseI | GCWGC | 5 cut(s) | 772, 805, 1781, 2319, 2741 |
| Tsp45I | GTSAC | 3 cut(s) | 615, 1739, 1751 |
| TspGWI | ACGGA | 2 cut(s) | 1103, 2429 |
| TspRI | CASTG | 1 cut(s) | 1914 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2390 |
| VpaK11BI | GGWCC | 4 cut(s) | 633, 1090, 1576, 2009 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1265, 2004 |
| XceI | RCATGY | 6 cut(s) | 335, 685, 1377, 1835, 2714, 2739 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1146 |
| XhoI | CTCGAG | 1 cut(s) | 2873 |
| XmiI | GTMKAC | 2 cut(s) | 2489, 2540 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2844 |
| XspI | CTAG | 4 cut(s) | 1008, 1526, 1952, 2862 |