Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 399 |
| Acc36I | ACCTGC | 1 cut(s) | 441 |
| AccB1I | GGYRCC | 1 cut(s) | 766 |
| AciI | CCGC | 3 cut(s) | 353, 713, 814 |
| AcsI | RAATTY | 4 cut(s) | 528, 723, 779, 830 |
| AcyI | GRCGYC | 1 cut(s) | 396 |
| AfaI | GTAC | 2 cut(s) | 930, 963 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 283 |
| AflIII | ACRYGT | 1 cut(s) | 747 |
| AgsI | TTSAA | 7 cut(s) | 214, 527, 557, 611, 729, 805, 823 |
| AluBI | AGCT | 2 cut(s) | 251, 295 |
| AluI | AGCT | 2 cut(s) | 251, 295 |
| Alw21I | GWGCWC | 2 cut(s) | 82, 702 |
| Alw26I | GTCTC | 1 cut(s) | 62 |
| ApeKI | GCWGC | 2 cut(s) | 295, 355 |
| ApoI | RAATTY | 4 cut(s) | 528, 723, 779, 830 |
| AspS9I | GGNCC | 1 cut(s) | 31 |
| AsuHPI | GGTGA | 1 cut(s) | 803 |
| AsuII | TTCGAA | 1 cut(s) | 666 |
| AvaII | GGWCC | 1 cut(s) | 31 |
| BanI | GGYRCC | 1 cut(s) | 766 |
| BbsI | GAAGAC | 1 cut(s) | 324 |
| Bbv12I | GWGCWC | 2 cut(s) | 82, 702 |
| BbvI | GCAGC | 2 cut(s) | 282, 367 |
| BceAI | ACGGC | 2 cut(s) | 345, 863 |
| BcoDI | GTCTC | 1 cut(s) | 62 |
| BfaI | CTAG | 3 cut(s) | 278, 447, 851 |
| BfmI | CTRYAG | 1 cut(s) | 683 |
| BfuAI | ACCTGC | 1 cut(s) | 441 |
| BisI | GCNGC | 4 cut(s) | 296, 353, 356, 713 |
| BlsI | GCNGC | 4 cut(s) | 297, 354, 357, 714 |
| BmcAI | AGTACT | 1 cut(s) | 963 |
| Bme18I | GGWCC | 1 cut(s) | 31 |
| BmgT120I | GGNCC | 1 cut(s) | 31 |
| BmiI | GGNNCC | 3 cut(s) | 93, 107, 768 |
| BmsI | GCATC | 2 cut(s) | 47, 600 |
| BpiI | GAAGAC | 1 cut(s) | 324 |
| BpmI | CTGGAG | 1 cut(s) | 594 |
| Bpu10I | CCTNAGC | 1 cut(s) | 363 |
| Bpu14I | TTCGAA | 1 cut(s) | 666 |
| BsaHI | GRCGYC | 1 cut(s) | 396 |
| BsaJI | CCNNGG | 1 cut(s) | 624 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 83, 113, 567, 597, 856, 886 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 283 |
| Bse1I | ACTGG | 1 cut(s) | 387 |
| BseDI | CCNNGG | 1 cut(s) | 624 |
| BseGI | GGATG | 1 cut(s) | 174 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 283 |
| BseNI | ACTGG | 1 cut(s) | 387 |
| BseRI | GAGGAG | 2 cut(s) | 320, 323 |
| BseXI | GCAGC | 2 cut(s) | 282, 367 |
| BshNI | GGYRCC | 1 cut(s) | 766 |
| BsiHKAI | GWGCWC | 2 cut(s) | 82, 702 |
| BslFI | GGGAC | 3 cut(s) | 383, 422, 663 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 283 |
| BsmAI | GTCTC | 1 cut(s) | 62 |
| BsmFI | GGGAC | 3 cut(s) | 383, 422, 663 |
| Bsp119I | TTCGAA | 1 cut(s) | 666 |
| Bsp1286I | GDGCHC | 2 cut(s) | 82, 702 |
| Bsp143I | GATC | 1 cut(s) | 796 |
| BspACI | CCGC | 3 cut(s) | 353, 713, 814 |
| BspLI | GGNNCC | 3 cut(s) | 93, 107, 768 |
| BspMI | ACCTGC | 1 cut(s) | 441 |
| BspQI | GCTCTTC | 1 cut(s) | 285 |
| BspT104I | TTCGAA | 1 cut(s) | 666 |
| BspT107I | GGYRCC | 1 cut(s) | 766 |
| BsrI | ACTGG | 1 cut(s) | 387 |
| BssECI | CCNNGG | 1 cut(s) | 624 |
| BssMI | GATC | 1 cut(s) | 796 |
| BssNI | GRCGYC | 1 cut(s) | 396 |
| BssT1I | CCWWGG | 1 cut(s) | 624 |
| Bst4CI | ACNGT | 1 cut(s) | 687 |
| Bst6I | CTCTTC | 2 cut(s) | 285, 468 |
| BstACI | GRCGYC | 1 cut(s) | 396 |
| BstBI | TTCGAA | 1 cut(s) | 666 |
| BstDEI | CTNAG | 1 cut(s) | 363 |
| BstF5I | GGATG | 1 cut(s) | 174 |
| BstKTI | GATC | 1 cut(s) | 799 |
| BstMAI | GTCTC | 1 cut(s) | 62 |
| BstMBI | GATC | 1 cut(s) | 796 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 47, 364 |
| BstNSI | RCATGY | 3 cut(s) | 19, 325, 751 |
| BstSFI | CTRYAG | 1 cut(s) | 683 |
| BstV1I | GCAGC | 2 cut(s) | 282, 367 |
| BstV2I | GAAGAC | 1 cut(s) | 324 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 102 |
| BtsCI | GGATG | 1 cut(s) | 174 |
| BveI | ACCTGC | 1 cut(s) | 441 |
| Cfr13I | GGNCC | 1 cut(s) | 31 |
| Csp6I | GTAC | 2 cut(s) | 929, 962 |
| CviAII | CATG | 6 cut(s) | 16, 157, 162, 322, 408, 748 |
| CviQI | GTAC | 2 cut(s) | 929, 962 |
| DdeI | CTNAG | 1 cut(s) | 363 |
| DpnI | GATC | 1 cut(s) | 798 |
| DpnII | GATC | 1 cut(s) | 796 |
| DraI | TTTAAA | 2 cut(s) | 136, 784 |
| Eam1104I | CTCTTC | 2 cut(s) | 285, 468 |
| EarI | CTCTTC | 2 cut(s) | 285, 468 |
| Eco130I | CCWWGG | 1 cut(s) | 624 |
| Eco47I | GGWCC | 1 cut(s) | 31 |
| EcoT14I | CCWWGG | 1 cut(s) | 624 |
| EcoT22I | ATGCAT | 2 cut(s) | 62, 167 |
| ErhI | CCWWGG | 1 cut(s) | 624 |
| FaeI | CATG | 6 cut(s) | 19, 160, 165, 325, 411, 751 |
| FaqI | GGGAC | 3 cut(s) | 383, 422, 663 |
| FatI | CATG | 6 cut(s) | 15, 156, 161, 321, 407, 747 |
| Fnu4HI | GCNGC | 4 cut(s) | 296, 353, 356, 713 |
| FokI | GGATG | 1 cut(s) | 161 |
| Fsp4HI | GCNGC | 4 cut(s) | 296, 353, 356, 713 |
| FspBI | CTAG | 3 cut(s) | 278, 447, 851 |
| GluI | GCNGC | 4 cut(s) | 296, 353, 356, 713 |
| GsuI | CTGGAG | 1 cut(s) | 594 |
| Hin1I | GRCGYC | 1 cut(s) | 396 |
| Hin1II | CATG | 6 cut(s) | 19, 160, 165, 325, 411, 751 |
| HinfI | GANTC | 3 cut(s) | 460, 553, 760 |
| HphI | GGTGA | 1 cut(s) | 803 |
| Hpy188I | TCNGA | 1 cut(s) | 418 |
| Hpy188III | TCNNGA | 2 cut(s) | 557, 776 |
| HpyAV | CCTTC | 5 cut(s) | 511, 556, 581, 662, 850 |
| HpyCH4III | ACNGT | 1 cut(s) | 687 |
| HpyCH4IV | ACGT | 2 cut(s) | 396, 937 |
| HpyCH4V | TGCA | 7 cut(s) | 21, 60, 165, 325, 514, 539, 878 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 47, 364 |
| HpyF3I | CTNAG | 1 cut(s) | 363 |
| HpySE526I | ACGT | 2 cut(s) | 396, 937 |
| Hsp92I | GRCGYC | 1 cut(s) | 396 |
| Hsp92II | CATG | 6 cut(s) | 19, 160, 165, 325, 411, 751 |
| Kzo9I | GATC | 1 cut(s) | 796 |
| LguI | GCTCTTC | 1 cut(s) | 285 |
| LmnI | GCTCC | 6 cut(s) | 47, 77, 91, 248, 697, 894 |
| LpnPI | CCDG | 7 cut(s) | 400, 436, 558, 690, 749, 761, 807 |
| Lsp1109I | GCAGC | 2 cut(s) | 282, 367 |
| LweI | GCATC | 2 cut(s) | 47, 600 |
| MaeI | CTAG | 3 cut(s) | 278, 447, 851 |
| MaeII | ACGT | 2 cut(s) | 396, 937 |
| MalI | GATC | 1 cut(s) | 798 |
| MboI | GATC | 1 cut(s) | 796 |
| MboII | GAAGA | 5 cut(s) | 302, 326, 329, 485, 590 |
| MhlI | GDGCHC | 2 cut(s) | 82, 702 |
| MluCI | AATT | 8 cut(s) | 300, 402, 528, 618, 723, 729, 779, 830 |
| MmeI | TCCRAC | 3 cut(s) | 83, 226, 613 |
| MnlI | CCTC | 8 cut(s) | 235, 280, 298, 301, 304, 379, 592, 726 |
| Mph1103I | ATGCAT | 2 cut(s) | 62, 167 |
| MseI | TTAA | 5 cut(s) | 135, 755, 783, 912, 967 |
| MslI | CAYNNNNRTG | 1 cut(s) | 902 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 47, 364 |
| NdeII | GATC | 1 cut(s) | 796 |
| NlaIII | CATG | 6 cut(s) | 19, 160, 165, 325, 411, 751 |
| NlaIV | GGNNCC | 3 cut(s) | 93, 107, 768 |
| NsiI | ATGCAT | 2 cut(s) | 62, 167 |
| NspI | RCATGY | 3 cut(s) | 19, 325, 751 |
| NspV | TTCGAA | 1 cut(s) | 666 |
| PciI | ACATGT | 1 cut(s) | 747 |
| PciSI | GCTCTTC | 1 cut(s) | 285 |
| PfeI | GAWTC | 3 cut(s) | 460, 553, 760 |
| PkrI | GCNGC | 4 cut(s) | 297, 354, 357, 714 |
| PscI | ACATGT | 1 cut(s) | 747 |
| PspN4I | GGNNCC | 3 cut(s) | 93, 107, 768 |
| PspPI | GGNCC | 1 cut(s) | 31 |
| RsaI | GTAC | 2 cut(s) | 930, 963 |
| RsaNI | GTAC | 2 cut(s) | 929, 962 |
| RseI | CAYNNNNRTG | 1 cut(s) | 902 |
| SapI | GCTCTTC | 1 cut(s) | 285 |
| SaqAI | TTAA | 5 cut(s) | 135, 755, 783, 912, 967 |
| SatI | GCNGC | 4 cut(s) | 296, 353, 356, 713 |
| Sau3AI | GATC | 1 cut(s) | 796 |
| Sau96I | GGNCC | 1 cut(s) | 31 |
| ScaI | AGTACT | 1 cut(s) | 963 |
| SduI | GDGCHC | 2 cut(s) | 82, 702 |
| SfaNI | GCATC | 2 cut(s) | 47, 600 |
| SfcI | CTRYAG | 1 cut(s) | 683 |
| SfuI | TTCGAA | 1 cut(s) | 666 |
| SinI | GGWCC | 1 cut(s) | 31 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 902 |
| Sse9I | AATT | 8 cut(s) | 300, 402, 528, 618, 723, 729, 779, 830 |
| SsiI | CCGC | 3 cut(s) | 353, 713, 814 |
| SspMI | CTAG | 3 cut(s) | 278, 447, 851 |
| StyI | CCWWGG | 1 cut(s) | 624 |
| TaaI | ACNGT | 1 cut(s) | 687 |
| TaiI | ACGT | 2 cut(s) | 399, 940 |
| TaqI | TCGA | 1 cut(s) | 666 |
| TasI | AATT | 8 cut(s) | 300, 402, 528, 618, 723, 729, 779, 830 |
| TatI | WGTACW | 1 cut(s) | 961 |
| TauI | GCSGC | 2 cut(s) | 355, 715 |
| TfiI | GAWTC | 3 cut(s) | 460, 553, 760 |
| Tru1I | TTAA | 5 cut(s) | 135, 755, 783, 912, 967 |
| Tru9I | TTAA | 5 cut(s) | 135, 755, 783, 912, 967 |
| TseI | GCWGC | 2 cut(s) | 295, 355 |
| TspDTI | ATGAA | 2 cut(s) | 17, 679 |
| VpaK11BI | GGWCC | 1 cut(s) | 31 |
| XapI | RAATTY | 4 cut(s) | 528, 723, 779, 830 |
| XceI | RCATGY | 3 cut(s) | 19, 325, 751 |
| XspI | CTAG | 3 cut(s) | 278, 447, 851 |
| ZraI | GACGTC | 1 cut(s) | 397 |
| ZrmI | AGTACT | 1 cut(s) | 963 |
| Zsp2I | ATGCAT | 2 cut(s) | 62, 167 |