pycom13g27570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000120
Physical Location & Seq
Forward (+)
23769892 .. 23775761
5870 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g27570.19

Sequence Viewer

Length: 4311 bp
ATGGAAGATGCATATGTGCCTTTTCAGGATCATATATGGTGCCCATCCTTCTTATCCTTTAATGGTCCTCTTATAGTTGGAGATTCTATGATGAAGAATGATATAACTGCTATAATAGTAGCTAAGAATCTCACTCCCAAAGATAAAAGAATACTTTCAAAAAGGTTTGATGAATTGTTCCTATCCAACAGGAGCCAACGTCTACTTGCCCAAGCTCGCCAAGTTGAATCATTAATGACTGAGGTGGCAAGTCTTAGACAAGAGATTAGAGGGCTCAAGCATGAGAATATAGAGTTGCACATACTTACAAATAATTACTCGATGAACATGAAAAGAAAGCTCGACCAGCTGCAAGAATCTGAAAGTTGGATTCAAAGTCAGAGGTTCATTATGCCAAGTACTAAGGCTTCACACGATCAACCTCCGTTGCTTCAAATGATCAACCTTTGTTGCCTTCCCATTTTGGAGTTTCGCCAAGTACTGAAGCTTCAAATGATCAACGTTTGTGAAAGCTCTTTTTTCTTTTTTGTTTTTCCTTTCTGTATTGTAACTCATGTGTGTACATGTGTGTGTGTGTGTACATATATATATATATATATATATATATATAGAAAAATTTGTTGCACCATTCGCACATACACACGTGCGCGCGCGCGCACACACACACACACACACACACAGATATATATATATATATATATATATATATATATATATATTAATAAAGGAATGGCGATGAATCTGCACCATTCATACTATAATAATATATACATTATAGATTCATTAACTTACTATACATCCCTTTTTCTTTCTAACTGTGGTCATGTATCAACGGTAGTGTTTGAGAATTATAGGAAGATCAAACAAAAGTGTTTCCATCTGAGGTCAGGAAGTTGGAGTTTAAAAGTCGTTAACTTGGTTGTTAGTTGTTATGTAGATTTCAGGTCTATAGTAGTATTTATAAGATGTGGAATGAAATCTTTCAAGAATTTTGATATGTTATGGGAGACAGGATATACAACTTTAAGAAGGAAACATTTATGTTTAAGCATAGGAAGTCGGAGTTTTAGAACTCGTAACACGACTGTTGGTTTTTCAGATATACAATCATATAACAAGATATATACTTTAAGAAAATTAGTCTTATTGAGATATGATCATAAAATGAGAAGATTCTCTTATTTATTTATGGCATTGTGCTTTCCACTAACCCGAGAGATTCTCGTGCTGATAACGTGTTGTGAAATTGAGGATATGTGTGTAGTGAAATATAAAGTAAATAAGACAATATTTACAAGGTTCGGCAAATGTTCCTACGTCCTTGGGATCTTTCACTATTTTTCTTTGTTCTCTAGCCTTATATTTTCTTCATTCCATCTCACCTTTCCATTAGTCAATAATAGTAGGGTAAACTACACAAAACTACCCCAACTATGGGTCAAACCACAATCTCATATCTCATGTTTTAAACATTCTCCGTTAAATTTTCTATCAATTTGGCTATTAAATGATGACGTGGTAGGAGGGGGCCCTCCCTTTTTTTATCAACATAAATTAATTAAAAAAATTCTCTCCCTCTGGCCAGATTTTGCAAGGAAGATTACCAGAAAATTCCGGCTCCGTTCAACACTCAAATCTCCGCTGAGCCCTGACCCACATGTTTACAGTAAAAACCTCAATCACTCACTCGAACTTTTTCACCCTTTTATTGGGGAGTTGGATTTTGAAGGATTACCAATGTCGAGGAGCAGCTTGGGGATTGCGAGTGTACTTGCAAGCTTCCTCTTCATTGTAGAATATCTTAGGTGGAGCTTGCAGCTCCGACCGATACATGTTTATTTTTCTCCTCTATTTCACAAGAGGGTGGAAGAGATGGGGGAGAAGAGAGAGAACTTAAACCCTAGCAACGTTCTCAGGCCATTCTTTGGCCACAGCTTGGCCTTAAGTGTTGAACGGAGCCGGAATTTTTTGGTAATCTTCCTTTTTTTATTTAAGTTGACGAATGAGGGGCCCGCTCTTGCCACGTCATCGTTTAACAACCAAATTAACAGAAAAATTAACAGAGGTATAACATTGCAACGAATTCATAAGTTGAGGTATGACATTGCAATTAAGGTAGTTTTAATTAATTTACCCATAATATTAAATACAAAATCTTCACGTGGGTGCTGCTTTTATAGAAAGACGCACCCAGATCCCTTCAACTACCCCCACTCCCATCGAGTACACCACATACTCCCAAGTTTAAACCTAAAATCCCAAATCGAGAAGCTTAGAACCCTAGCGCCCCTGAAGGAGGAATTCTCTAAGAGAAACAAAGAGGAAGATAACCTAGAAATCCGTTGGATCCATTGCCAACATACTATAAGTGATTTGTGCATTGTTTTTTTATTTGTAAGAAACAAAGTAAATCGGTTGGAGGTTCAAAAAAATTTGAGAAATGTTGGGGTTTGGTCTCTATTTAGTCTTGTTAGAAATGTTATTGTTCGATCTCTGTTTAATCTTGTTGTAAAAAGCATGTGGGTTATTTTTCTTGTAGTGCCTTGTGGTTTAACAAGTTTGAAAATAACAATTTTCTATGTCTTTTTCTACTTTGTCTGTAATGTGCTTTTGGGGTGTCATTCCAAAAATCTCATTCTACACTCTAAACTTTCTATATTAGAAAAGAAAAATACATTTATGAAGAATTCATATCAAAATGTGGTCCTAAAATTTGTTTGTATAGTAAAGTTGCACTTTGTTTTTCCCAGGGTTTATTCAGGGACCAACAAACTTTTCACTACCAAAATGTATCATGGTAGGGGTTCAATGATGATTTTTATGTTGGAGGCAAGGTTGATTTTTTTTATTTTTTATTTTTTATTTTTACTGTTGCAACAAAGAAGATGCCAACCCTTGATGGTAAATCCTAATGTGATTGATATGTGCAAGTTTGTGCCAAAGCACATGATGATTCACTTAGAGTTTATTAAGTCCTTTGAGCTGGTTGCTATGAGTGATGTGGTTATCCAAGAAATTAGTGAAGATGAGGATGATCAAAGGAGGCTAGTGCAAGTTAATTGTGCCTTCTCAAGCTGTGAATCACATGGAGACGATGATCACATAGATATAGAGAGGAAAACAAGCAGCATAAAGTTTGAACAAGACATTGATGTAGACGTTGGTGAGCCAGACTATAATTCAGATGGCCTTTATAGTGTCCATTCTGATGAAGATGTAAGAGACAACAATAATAAGCCATATCCTGAGTTCAACGAGGAGACCGACATGAAGAACTCACAATTGTCACTAAGACTGATATTTAGGGATGTGCAAAGTTTAGGAAGCTTGCATAAAACAATGTCCTTGGTAAGATGTGTAATTCCAACAACATACATATTAAAACATTGCTTGATTTTCACAAATGTGAGGATTGTGGGTCAATGGGAATATGAAAGTCTTGTCTTACTGAAACATACGTGGAAAGGATCCAACTTAATCCAACTTGGCATGTTGATTTTTTGTTGCTATAGGGTAATGACTGCTGTTTTGAAGATTATTGAGGGTAAACATGTTGGGCATTACACAAAATTATGGGATTTTGCTGAAGAAGTGAAGAAGAAAAACCCATGGAGCACTCTAAAAATTAAACTGAATAAAGGAAGGTTCCAGAGGATGTGTCTGTTTTGCTGCATGGAAGGAAGGATTCAAGTCTCAACATGGGGGTTAATTATTGAGCATTGTTGGGATAGATCCCAACAACGAGACTCGGTGGTTAAGCTTATTTATTTTTGGCTGATGACGTTGAGATTAGGATTCTTCCTAAATGCCACCACAGGTTTTGTGTGCGGCATTTGTATACAAACTACAAAGAACAAATTCAAGGGCAAACCATACTTCAAAAGAGAAATTGAGGAACTTAAATTGTTGAGCAAAGATGCGTTTGATTGGTTCATGAAGAGGCCTACCATTTACTGGAGTAAATCTCATTTTTTAACTTTTACCAAGTGTGAAATTTTGTTCAACAATCTGTGTGAAAGCTTTAATACTGTGATATTGAATAGAATGTCCAAGCCAATCATAAATGCTTCAAATCATTCATACCATGTTGAAGAAAAGAATTCAAATGAGGAGAGACATTATAGGGAGGAAAATGAAGATTTGAGCCTCAAGATTAAGAAGAAGTTGGAGGAGGCAAAGATAGGGAGTGGTCGGTGTATGAACGAACATGTTCATGTAAAAAATATGATCTCACAGGCATACAATGCCATTGTTGCCAGTAATTACAAGAGAGATAAACTAGAAGACTATATAGATGCTTGTTATTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

1437

Amino Acids

169.09

Weight (kDa)

9.38

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000120)

Species Orthologous Gene IDs
malus_domestica MD00G1178700.v1.1 MD00G1192600.v1.1 MD01G1178800.v1.1 MD08G1118400.v1.1 MD11G1121800.v1.1 MD11G1175900.v1.1 MD14G1073500.v1.1 MD16G1171800.v1.1
prunus_persica Prupe.5G057200_v2.0.a1 Prupe.7G095400_v2.0.a1 Prupe.7G095500_v2.0.a1 Prupe.8G037200_v2.0.a1
pyrus_communis pycom01g00040 pycom01g00270 pycom01g00550 pycom01g00730 pycom01g00740 pycom01g00820 pycom01g00960 pycom01g01060 pycom01g01180 pycom01g01580 pycom01g01700 pycom01g01770 pycom01g01970 pycom01g02520 pycom01g03120 pycom01g03510 pycom01g07770 pycom01g07800 pycom01g11420 pycom02g06240 pycom02g13440 pycom02g16920 pycom02g17900 pycom02g18220 pycom02g21560 pycom03g10720 pycom03g11710 pycom03g11930 pycom04g05770 pycom04g07600 pycom04g07920 pycom04g07980 pycom04g08250 pycom04g09780 pycom04g12720 pycom04g13700 pycom05g00180 pycom05g01110 pycom05g05940 pycom05g06250 pycom05g06870 pycom05g08470 pycom05g08920 pycom05g09050 pycom05g09420 pycom05g14090 pycom06g05150 pycom06g05430 pycom06g06950 pycom06g09990 pycom06g15520 pycom07g08780 pycom07g11240 pycom07g11990 pycom07g14400 pycom07g27820 pycom08g07740 pycom08g10980 pycom08g12370 pycom09g03580 pycom09g09700 pycom09g13450 pycom09g14910 pycom10g01230 pycom10g02170 pycom10g06850 pycom10g08040 pycom10g08250 pycom10g10100 pycom10g15420 pycom1125g00010 pycom11g04190 pycom11g14150 pycom11g15090 pycom11g15720 pycom11g16230 pycom11g17180 pycom11g20110 pycom1236g00020 pycom12426g00290 pycom12426g00350 pycom12426g00660 pycom12426g00700 pycom12462g00070 pycom12463g00060 pycom12473g00010 pycom12503g00010 pycom12517g00150 pycom1256g00180 pycom1256g00220 pycom12575g00030 pycom12581g00010 pycom12596g00090 pycom12612g00020 pycom12621g00090 pycom12633g00040 pycom12637g00020 pycom12658g00010 pycom12658g00020 pycom12659g00070 pycom12668g00040 pycom12668g00050 pycom12674g00040 pycom12681g00050 pycom12681g00060 pycom12690g00020 pycom12695g00020 pycom12g08040 pycom12g08660 pycom12g09550 pycom12g09560 pycom13g03230 pycom13g18200 pycom13g19230 pycom13g20140 pycom13g20700 pycom13g22210 pycom13g23170 pycom13g25290 pycom13g25590 pycom13g25980 pycom13g26010 pycom13g26280 pycom13g26350 pycom13g27560 pycom13g27570 pycom13g27680 pycom13g27780 pycom13g28070 pycom13g28140 pycom13g28230 pycom13g28280 pycom13g29100 pycom1498g00010 pycom14g01730 pycom14g08250 pycom14g08660 pycom14g09260 pycom14g18640 pycom15g28640 pycom15g30060 pycom15g35030 pycom15g37780 pycom1604g00160 pycom1609g00100 pycom1681g00030 pycom1683g00020 pycom16g13500 pycom16g17920 pycom16g18140 pycom16g19340 pycom16g20200 pycom16g21370 pycom16g22140 pycom16g23170 pycom16g23450 pycom16g25040 pycom16g25790 pycom1729g00130 pycom1729g00180 pycom1739g00060 pycom1774g00010 pycom17g06230 pycom17g15230 pycom17g16620 pycom17g17340 pycom17g17660 pycom17g17810 pycom17g18150 pycom17g19280 pycom17g25050 pycom17g25100 pycom17g25680 pycom17g25690 pycom1880g00030 pycom1912g00020 pycom2002g00120 pycom2002g00160 pycom2177g00180 pycom2185g00070 pycom2189g00010 pycom2218g00030 pycom2320g00010 pycom2328g00040 pycom2339g00100 pycom2424g00030 pycom2466g00010 pycom2552g00010 pycom2599g00010 pycom2627g00040 pycom2749g00080 pycom2789g00010 pycom3027g00010 pycom394g00080 pycom436g00190 pycom436g00310 pycom436g00350 pycom436g00410 pycom436g00420 pycom436g00430 pycom443g00010 pycom505g00030 pycom520g00140 pycom520g00220 pycom520g00620 pycom520g00650 pycom555g00010 pycom57g00030 pycom608g00020 pycom723g00040 pycom775g00040 pycom808g00350 pycom808g00590 pycom808g00640 pycom889g00030 pycom908g00040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 962
AccB1I GGYRCC 1 cut(s) 39
AccB7I CCANNNNNTGG 3 cut(s) 1925, 1936, 3954
AccBSI CCGCTC 1 cut(s) 2015
AccI GTMKAC 3 cut(s) 202, 3156, 3838
AccII CGCG 4 cut(s) 649, 651, 653, 655
AciI CCGC 3 cut(s) 1640, 2013, 3828
AclI AACGTT 2 cut(s) 501, 1908
AclWI GGATC 8 cut(s) 36, 1334, 2189, 2341, 2354, 3462, 3475, 3726
AcoI YGGCCR 2 cut(s) 1580, 1927
AcuI CTGAAG 3 cut(s) 503, 2312, 3606
AcvI CACGTG 2 cut(s) 644, 2162
AfaI GTAC 6 cut(s) 400, 480, 562, 580, 1770, 2226
AfiI CCNNNNNNNGG 7 cut(s) 1434, 1435, 1709, 1925, 1936, 2296, 3954
AflII CTTAAG 1 cut(s) 1942
AflIII ACRYGT 7 cut(s) 563, 641, 1235, 1657, 1831, 3550, 4207
AjiI CACGTC 2 cut(s) 1516, 2025
AjnI CCWGG 1 cut(s) 2747
AjuI GAANNNNNNNTTGG 2 cut(s) 2759, 2791
AleI CACNNNNGTG 2 cut(s) 2164, 3404
Alw21I GWGCWC 1 cut(s) 3617
Alw26I GTCTC 8 cut(s) 1001, 2460, 3084, 3216, 3254, 3697, 3738, 4108
AlwI GGATC 8 cut(s) 36, 1334, 2189, 2341, 2354, 3462, 3475, 3726
Ama87I CYCGRG 1 cut(s) 1212
AoxI GGCC 8 cut(s) 1528, 1580, 1916, 1927, 1938, 2009, 3187, 3941
ApaI GGGCCC 2 cut(s) 1532, 2013
ApeKI GCWGC 6 cut(s) 349, 1749, 1816, 2169, 3126, 3669
ArsI GACNNNNNNTTYG 2 cut(s) 3511, 3543
AseI ATTAAT 4 cut(s) 233, 720, 1556, 2127
Asp700I GAANNNNTTC 5 cut(s) 154, 980, 1695, 3857, 4209
AspLEI GCGC 6 cut(s) 649, 651, 653, 655, 657, 2287
AspS9I GGNCC 7 cut(s) 65, 1528, 1529, 2009, 2010, 2704, 2763
AsuHPI GGTGA 3 cut(s) 1372, 1691, 3176
AvaI CYCGRG 1 cut(s) 1212
AvaII GGWCC 3 cut(s) 65, 2704, 2763
BaeGI GKGCMC 3 cut(s) 44, 1532, 2013
BalI TGGCCA 2 cut(s) 1582, 1929
BamHI GGATCC 2 cut(s) 2346, 3467
BanI GGYRCC 1 cut(s) 39
BanII GRGCYC 4 cut(s) 276, 1532, 1649, 2013
BarI GAAGNNNNNNTAC 4 cut(s) 391, 423, 471, 503
BauI CACGAG 1 cut(s) 1223
BbrPI CACGTG 2 cut(s) 644, 2162
BbsI GAAGAC 1 cut(s) 4290
Bbv12I GWGCWC 1 cut(s) 3617
BbvI GCAGC 6 cut(s) 336, 1761, 1828, 2156, 3138, 3656
BccI CCATC 7 cut(s) 52, 885, 1383, 1867, 2226, 2893, 3179
BcgI CGANNNNNNTGC 2 cut(s) 724, 758
BciT130I CCWGG 1 cut(s) 2749
BclI TGATCA 5 cut(s) 438, 495, 1156, 3034, 3097
BcoDI GTCTC 8 cut(s) 1001, 2460, 3084, 3216, 3254, 3697, 3738, 4108
BfaI CTAG 7 cut(s) 1353, 1902, 2282, 2333, 3047, 4280, 4309
BfmI CTRYAG 2 cut(s) 948, 3508
BfoI RGCGCY 1 cut(s) 2288
BfrI CTTAAG 1 cut(s) 1942
BisI GCNGC 7 cut(s) 350, 1750, 1817, 2170, 3127, 3670, 3829
BlpI GCTNAGC 1 cut(s) 1643
BlsI GCNGC 7 cut(s) 351, 1751, 1818, 2171, 3128, 3671, 3830
BmcAI AGTACT 2 cut(s) 400, 480
Bme1390I CCNGG 1 cut(s) 2749
Bme18I GGWCC 3 cut(s) 65, 2704, 2763
BmeT110I CYCGRG 1 cut(s) 1212
BmgBI CACGTC 2 cut(s) 1516, 2025
BmgT120I GGNCC 7 cut(s) 65, 1528, 1529, 2009, 2010, 2704, 2763
BmrFI CCNGG 1 cut(s) 2749
BmsI GCATC 3 cut(s) 2876, 3907, 4285
BpiI GAAGAC 1 cut(s) 4290
BplI GAGNNNNNCTC 6 cut(s) 1206, 1238, 2288, 2320, 3949, 3981
BpmI CTGGAG 1 cut(s) 3976
Bpu1102I GCTNAGC 1 cut(s) 1643
BpuEI CTTGAG 3 cut(s) 260, 3055, 4133
BsaAI YACGTR 3 cut(s) 644, 2162, 3459
BsaI GGTCTC 2 cut(s) 2460, 3254
BsaJI CCNNGG 5 cut(s) 1321, 2747, 2748, 3345, 3608
BsaXI ACNNNNNCTCC 6 cut(s) 184, 214, 2198, 2228, 2819, 2849
Bsc4I CCNNNNNNNGG 7 cut(s) 1434, 1435, 1709, 1925, 1936, 2296, 3954
Bse1I ACTGG 2 cut(s) 3959, 4257
Bse3DI GCAATG 4 cut(s) 2072, 2103, 2350, 3385
BseBI CCWGG 1 cut(s) 2749
BseDI CCNNGG 5 cut(s) 1321, 2747, 2748, 3345, 3608
BseGI GGATG 5 cut(s) 44, 797, 3037, 3313, 3660
BseLI CCNNNNNNNGG 7 cut(s) 1434, 1435, 1709, 1925, 1936, 2296, 3954
BseMI GCAATG 4 cut(s) 2072, 2103, 2350, 3385
BseMII CTCAG 5 cut(s) 231, 872, 1634, 1927, 3237
BseNI ACTGG 2 cut(s) 3959, 4257
BsePI GCGCGC 4 cut(s) 647, 649, 651, 653
BseRI GAGGAG 5 cut(s) 1759, 1836, 3272, 4124, 4184
BseSI GKGCMC 3 cut(s) 44, 1532, 2013
BseXI GCAGC 6 cut(s) 336, 1761, 1828, 2156, 3138, 3656
BsgI GTGCAG 1 cut(s) 728
Bsh1236I CGCG 4 cut(s) 649, 651, 653, 655
Bsh1285I CGRYCG 1 cut(s) 1826
BshFI GGCC 8 cut(s) 1530, 1582, 1918, 1929, 1940, 2011, 3189, 3943
BshNI GGYRCC 1 cut(s) 39
BsiEI CGRYCG 1 cut(s) 1826
BsiHKAI GWGCWC 1 cut(s) 3617
BsiHKCI CYCGRG 1 cut(s) 1212
BsiSI CCGG 2 cut(s) 1615, 1960
BslFI GGGAC 1 cut(s) 2776
BslI CCNNNNNNNGG 7 cut(s) 1434, 1435, 1709, 1925, 1936, 2296, 3954
BsmAI GTCTC 8 cut(s) 1001, 2460, 3084, 3216, 3254, 3697, 3738, 4108
BsmBI CGTCTC 1 cut(s) 3084
BsmFI GGGAC 1 cut(s) 2776
BsnI GGCC 8 cut(s) 1530, 1582, 1918, 1929, 1940, 2011, 3189, 3943
Bso31I GGTCTC 2 cut(s) 2460, 3254
BsoBI CYCGRG 1 cut(s) 1212
Bsp120I GGGCCC 2 cut(s) 1528, 2009
Bsp1286I GDGCHC 6 cut(s) 44, 276, 1532, 1649, 2013, 3617
Bsp1407I TGTACA 2 cut(s) 560, 578
Bsp1720I GCTNAGC 1 cut(s) 1643
Bsp19I CCATGG 1 cut(s) 3608
BspACI CCGC 3 cut(s) 1640, 2013, 3828
BspANI GGCC 8 cut(s) 1530, 1582, 1918, 1929, 1940, 2011, 3189, 3943
BspCNI CTCAG 5 cut(s) 232, 873, 1635, 1926, 3238
BspFNI CGCG 4 cut(s) 649, 651, 653, 655
BspHI TCATGA 1 cut(s) 3933
BspPI GGATC 8 cut(s) 36, 1334, 2189, 2341, 2354, 3462, 3475, 3726
BspT107I GGYRCC 1 cut(s) 39
BspTI CTTAAG 1 cut(s) 1942
BspTNI GGTCTC 2 cut(s) 2460, 3254
BsrBI CCGCTC 1 cut(s) 2015
BsrDI GCAATG 4 cut(s) 2072, 2103, 2350, 3385
BsrGI TGTACA 2 cut(s) 560, 578
BsrI ACTGG 2 cut(s) 3959, 4257
BssECI CCNNGG 5 cut(s) 1321, 2747, 2748, 3345, 3608
BssHII GCGCGC 4 cut(s) 647, 649, 651, 653
BssNAI GTATAC 1 cut(s) 3839
BssSI CACGAG 1 cut(s) 1223
BssT1I CCWWGG 3 cut(s) 1321, 3345, 3608
Bst1107I GTATAC 1 cut(s) 3839
Bst2BI CACGAG 1 cut(s) 1223
Bst2UI CCWGG 1 cut(s) 2749
Bst4CI ACNGT 6 cut(s) 818, 835, 1087, 1667, 2871, 4030
Bst6I CTCTTC 4 cut(s) 1790, 1863, 1877, 3932
BstAFI CTTAAG 1 cut(s) 1942
BstAPI GCANNNNNTGC 1 cut(s) 4244
BstAUI TGTACA 2 cut(s) 560, 578
BstBAI YACGTR 3 cut(s) 644, 2162, 3459
BstC8I GCNNGC 9 cut(s) 217, 649, 651, 653, 655, 1777, 1814, 2013, 3329
BstDSI CCRYGG 1 cut(s) 3608
BstENI CCTNNNNNAGG 1 cut(s) 2294
BstF5I GGATG 5 cut(s) 44, 797, 3037, 3313, 3660
BstFNI CGCG 4 cut(s) 649, 651, 653, 655
BstH2I RGCGCY 1 cut(s) 2288
BstHHI GCGC 6 cut(s) 649, 651, 653, 655, 657, 2287
BstMAI GTCTC 8 cut(s) 1001, 2460, 3084, 3216, 3254, 3697, 3738, 4108
BstMCI CGRYCG 1 cut(s) 1826
BstMWI GCNNNNNNNGC 4 cut(s) 346, 630, 4244, 4253
BstNI CCWGG 1 cut(s) 2749
BstNSI RCATGY 7 cut(s) 567, 1661, 1835, 2521, 3493, 3554, 4211
BstSCI CCNGG 1 cut(s) 2747
BstSFI CTRYAG 2 cut(s) 948, 3508
BstSLI GKGCMC 3 cut(s) 44, 1532, 2013
BstUI CGCG 4 cut(s) 649, 651, 653, 655
BstV1I GCAGC 6 cut(s) 336, 1761, 1828, 2156, 3138, 3656
BstV2I GAAGAC 1 cut(s) 4290
BstX2I RGATCY 5 cut(s) 1326, 2194, 2346, 3467, 3731
BstYI RGATCY 5 cut(s) 1326, 2194, 2346, 3467, 3731
BstZ17I GTATAC 1 cut(s) 3839
BsuRI GGCC 8 cut(s) 1530, 1582, 1918, 1929, 1940, 2011, 3189, 3943
BtgI CCRYGG 1 cut(s) 3608
BtgZI GCGATG 1 cut(s) 749
BtrI CACGTC 2 cut(s) 1516, 2025
BtsCI GGATG 5 cut(s) 44, 797, 3037, 3313, 3660
Cac8I GCNNGC 9 cut(s) 217, 649, 651, 653, 655, 1777, 1814, 2013, 3329
CciI TCATGA 1 cut(s) 3933
CfoI GCGC 6 cut(s) 649, 651, 653, 655, 657, 2287
Cfr13I GGNCC 7 cut(s) 65, 1528, 1529, 2009, 2010, 2704, 2763
CseI GACGC 1 cut(s) 2194
Csp6I GTAC 6 cut(s) 399, 479, 561, 579, 1769, 2225
CspCI CAANNNNNGTGG 2 cut(s) 3802, 3837
CviQI GTAC 6 cut(s) 399, 479, 561, 579, 1769, 2225
DraI TTTAAA 3 cut(s) 903, 1468, 2247
EaeI YGGCCR 2 cut(s) 1580, 1927
Eam1104I CTCTTC 4 cut(s) 1790, 1863, 1877, 3932
EarI CTCTTC 4 cut(s) 1790, 1863, 1877, 3932
Eco130I CCWWGG 3 cut(s) 1321, 3345, 3608
Eco147I AGGCCT 1 cut(s) 3943
Eco24I GRGCYC 4 cut(s) 276, 1532, 1649, 2013
Eco31I GGTCTC 2 cut(s) 2460, 3254
Eco47I GGWCC 3 cut(s) 65, 2704, 2763
Eco57I CTGAAG 3 cut(s) 503, 2312, 3606
Eco72I CACGTG 2 cut(s) 644, 2162
Eco88I CYCGRG 1 cut(s) 1212
EcoNI CCTNNNNNAGG 1 cut(s) 2294
EcoO109I RGGNCCY 3 cut(s) 1528, 1529, 2009
EcoRI GAATTC 4 cut(s) 2082, 2300, 2686, 4099
EcoRII CCWGG 1 cut(s) 2747
EcoT14I CCWWGG 3 cut(s) 1321, 3345, 3608
EcoT22I ATGCAT 1 cut(s) 13
EcoT38I GRGCYC 4 cut(s) 276, 1532, 1649, 2013
ErhI CCWWGG 3 cut(s) 1321, 3345, 3608
Esp3I CGTCTC 1 cut(s) 3084
FalI AAGNNNNNCTT 4 cut(s) 1162, 1194, 2720, 2752
FaqI GGGAC 1 cut(s) 2776
FauI CCCGC 1 cut(s) 2020
FauNDI CATATG 1 cut(s) 13
FbaI TGATCA 5 cut(s) 438, 495, 1156, 3034, 3097
FblI GTMKAC 3 cut(s) 202, 3156, 3838
Fnu4HI GCNGC 7 cut(s) 350, 1750, 1817, 2170, 3127, 3670, 3829
FokI GGATG 5 cut(s) 31, 784, 3044, 3320, 3667
FriOI GRGCYC 4 cut(s) 276, 1532, 1649, 2013
Fsp4HI GCNGC 7 cut(s) 350, 1750, 1817, 2170, 3127, 3670, 3829
FspBI CTAG 7 cut(s) 1353, 1902, 2282, 2333, 3047, 4280, 4309
GlaI GCGC 6 cut(s) 648, 650, 652, 654, 656, 2286
GluI GCNGC 7 cut(s) 350, 1750, 1817, 2170, 3127, 3670, 3829
GsuI CTGGAG 1 cut(s) 3976
HaeII RGCGCY 1 cut(s) 2288
HaeIII GGCC 8 cut(s) 1530, 1582, 1918, 1929, 1940, 2011, 3189, 3943
HapII CCGG 2 cut(s) 1615, 1960
HgaI GACGC 1 cut(s) 2194
HhaI GCGC 6 cut(s) 649, 651, 653, 655, 657, 2287
Hin6I GCGC 6 cut(s) 647, 649, 651, 653, 655, 2285
HinP1I GCGC 6 cut(s) 647, 649, 651, 653, 655, 2285
HincII GTYRAC 2 cut(s) 913, 1998
HindII GTYRAC 2 cut(s) 913, 1998
HindIII AAGCTT 6 cut(s) 485, 1777, 2270, 3325, 3758, 4018
HpaI GTTAAC 1 cut(s) 913
HpaII CCGG 2 cut(s) 1615, 1960
HphI GGTGA 3 cut(s) 1372, 1691, 3176
Hpy188I TCNGA 8 cut(s) 361, 381, 882, 1062, 1099, 1823, 3184, 3208
Hpy188III TCNNGA 8 cut(s) 26, 888, 985, 2266, 3245, 3649, 3934, 4150
HpyCH4III ACNGT 6 cut(s) 818, 835, 1087, 1667, 2871, 4030
HpyF10VI GCNNNNNNNGC 4 cut(s) 346, 630, 4244, 4253
HspAI GCGC 6 cut(s) 647, 649, 651, 653, 655, 2285
Ksp22I TGATCA 5 cut(s) 438, 495, 1156, 3034, 3097
KspAI GTTAAC 1 cut(s) 913
LmnI GCTCC 7 cut(s) 192, 1623, 1746, 1809, 1824, 1956, 3612
Lsp1109I GCAGC 6 cut(s) 336, 1761, 1828, 2156, 3138, 3656
LweI GCATC 3 cut(s) 2876, 3907, 4285
MaeI CTAG 7 cut(s) 1353, 1902, 2282, 2333, 3047, 4280, 4309
MaeIII GTNAC 3 cut(s) 547, 1076, 3285
MauBI CGCGCGCG 2 cut(s) 649, 651
MbiI CCGCTC 1 cut(s) 2015
MfeI CAATTG 1 cut(s) 3281
MflI RGATCY 5 cut(s) 1326, 2194, 2346, 3467, 3731
MhlI GDGCHC 6 cut(s) 44, 276, 1532, 1649, 2013, 3617
MlsI TGGCCA 2 cut(s) 1582, 1929
MluNI TGGCCA 2 cut(s) 1582, 1929
MlyI GAGTC 1 cut(s) 3740
Mox20I TGGCCA 2 cut(s) 1582, 1929
Mph1103I ATGCAT 1 cut(s) 13
MroXI GAANNNNTTC 5 cut(s) 154, 980, 1695, 3857, 4209
MscI TGGCCA 2 cut(s) 1582, 1929
MslI CAYNNNNRTG 5 cut(s) 568, 2164, 3207, 3404, 4212
Msp20I TGGCCA 2 cut(s) 1582, 1929
MspA1I CMGCKG 2 cut(s) 349, 1642
MspCI CTTAAG 1 cut(s) 1942
MspI CCGG 2 cut(s) 1615, 1960
MspR9I CCNGG 1 cut(s) 2749
MssI GTTTAAAC 1 cut(s) 2247
MunI CAATTG 1 cut(s) 3281
MvaI CCWGG 1 cut(s) 2749
MvnI CGCG 4 cut(s) 649, 651, 653, 655
MwoI GCNNNNNNNGC 4 cut(s) 346, 630, 4244, 4253
NcoI CCATGG 1 cut(s) 3608
NdeI CATATG 1 cut(s) 13
NmuCI GTSAC 1 cut(s) 3285
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 7 cut(s) 567, 1661, 1835, 2521, 3493, 3554, 4211
OliI CACNNNNGTG 2 cut(s) 2164, 3404
PacI TTAATTAA 2 cut(s) 1560, 2127
PagI TCATGA 1 cut(s) 3933
PasI CCCWGGG 1 cut(s) 2748
PauI GCGCGC 4 cut(s) 647, 649, 651, 653
PceI AGGCCT 1 cut(s) 3943
PciI ACATGT 5 cut(s) 563, 1657, 1831, 3550, 4207
PcsI WCGNNNNNNNCGW 1 cut(s) 3261
PdmI GAANNNNTTC 5 cut(s) 154, 980, 1695, 3857, 4209
PflMI CCANNNNNTGG 3 cut(s) 1925, 1936, 3954
PkrI GCNGC 7 cut(s) 351, 1751, 1818, 2171, 3128, 3671, 3830
PleI GAGTC 1 cut(s) 3740
PmaCI CACGTG 2 cut(s) 644, 2162
PmeI GTTTAAAC 1 cut(s) 2247
PmlI CACGTG 2 cut(s) 644, 2162
PpsI GAGTC 1 cut(s) 3740
Ppu21I YACGTR 3 cut(s) 644, 2162, 3459
PscI ACATGT 5 cut(s) 563, 1657, 1831, 3550, 4207
PshBI ATTAAT 4 cut(s) 233, 720, 1556, 2127
PsiI TTATAA 1 cut(s) 962
Psp1406I AACGTT 2 cut(s) 501, 1908
Psp6I CCWGG 1 cut(s) 2747
PspCI CACGTG 2 cut(s) 644, 2162
PspGI CCWGG 1 cut(s) 2747
PspOMI GGGCCC 2 cut(s) 1528, 2009
PspPI GGNCC 7 cut(s) 65, 1528, 1529, 2009, 2010, 2704, 2763
PsuI RGATCY 5 cut(s) 1326, 2194, 2346, 3467, 3731
PteI GCGCGC 4 cut(s) 647, 649, 651, 653
PvuII CAGCTG 1 cut(s) 349
RsaI GTAC 6 cut(s) 400, 480, 562, 580, 1770, 2226
RsaNI GTAC 6 cut(s) 399, 479, 561, 579, 1769, 2225
RseI CAYNNNNRTG 5 cut(s) 568, 2164, 3207, 3404, 4212
SatI GCNGC 7 cut(s) 350, 1750, 1817, 2170, 3127, 3670, 3829
Sau96I GGNCC 7 cut(s) 65, 1528, 1529, 2009, 2010, 2704, 2763
ScaI AGTACT 2 cut(s) 400, 480
SchI GAGTC 1 cut(s) 3740
ScrFI CCNGG 1 cut(s) 2749
SduI GDGCHC 6 cut(s) 44, 276, 1532, 1649, 2013, 3617
SfaNI GCATC 3 cut(s) 2876, 3907, 4285
SfcI CTRYAG 2 cut(s) 948, 3508
SinI GGWCC 3 cut(s) 65, 2704, 2763
SmiMI CAYNNNNRTG 5 cut(s) 568, 2164, 3207, 3404, 4212
SmlI CTYRAG 4 cut(s) 275, 1942, 3070, 4148
SmoI CTYRAG 4 cut(s) 275, 1942, 3070, 4148
SseBI AGGCCT 1 cut(s) 3943
SsiI CCGC 3 cut(s) 1640, 2013, 3828
SspI AATATT 2 cut(s) 1290, 2142
SspMI CTAG 7 cut(s) 1353, 1902, 2282, 2333, 3047, 4280, 4309
StuI AGGCCT 1 cut(s) 3943
StyD4I CCNGG 1 cut(s) 2747
StyI CCWWGG 3 cut(s) 1321, 3345, 3608
TaaI ACNGT 6 cut(s) 818, 835, 1087, 1667, 2871, 4030
TaqI TCGA 7 cut(s) 320, 342, 1689, 1742, 2221, 2265, 2488
TaqII GACCGA 2 cut(s) 1840, 3278
TatI WGTACW 6 cut(s) 398, 478, 560, 578, 1768, 2224
TauI GCSGC 1 cut(s) 3831
TseFI GTSAC 1 cut(s) 3285
TseI GCWGC 6 cut(s) 349, 1749, 1816, 2169, 3126, 3669
Tsp45I GTSAC 1 cut(s) 3285
TspGWI ACGGA 5 cut(s) 414, 1467, 1610, 1969, 2330
Van91I CCANNNNNTGG 3 cut(s) 1925, 1936, 3954
Vha464I CTTAAG 1 cut(s) 1942
VpaK11BI GGWCC 3 cut(s) 65, 2704, 2763
VspI ATTAAT 4 cut(s) 233, 720, 1556, 2127
XagI CCTNNNNNAGG 1 cut(s) 2294
XceI RCATGY 7 cut(s) 567, 1661, 1835, 2521, 3493, 3554, 4211
XmiI GTMKAC 3 cut(s) 202, 3156, 3838
XmnI GAANNNNTTC 5 cut(s) 154, 980, 1695, 3857, 4209
XspI CTAG 7 cut(s) 1353, 1902, 2282, 2333, 3047, 4280, 4309
ZrmI AGTACT 2 cut(s) 400, 480
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.