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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 178 |
| AsuHPI | GGTGA | 1 cut(s) | 143 |
| BccI | CCATC | 3 cut(s) | 23, 37, 337 |
| BceAI | ACGGC | 1 cut(s) | 85 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 111, 141 |
| Bse3DI | GCAATG | 3 cut(s) | 196, 238, 304 |
| BseMI | GCAATG | 3 cut(s) | 196, 238, 304 |
| BseRI | GAGGAG | 1 cut(s) | 37 |
| BslFI | GGGAC | 1 cut(s) | 76 |
| BsmFI | GGGAC | 1 cut(s) | 76 |
| BsrDI | GCAATG | 3 cut(s) | 196, 238, 304 |
| BstDEI | CTNAG | 1 cut(s) | 57 |
| BstNSI | RCATGY | 4 cut(s) | 213, 255, 279, 321 |
| CviAII | CATG | 4 cut(s) | 210, 252, 276, 318 |
| DdeI | CTNAG | 1 cut(s) | 57 |
| FaeI | CATG | 4 cut(s) | 213, 255, 279, 321 |
| FaiI | YATR | 6 cut(s) | 126, 161, 211, 253, 277, 319 |
| FaqI | GGGAC | 1 cut(s) | 76 |
| FatI | CATG | 4 cut(s) | 209, 251, 275, 317 |
| Hin1II | CATG | 4 cut(s) | 213, 255, 279, 321 |
| HinfI | GANTC | 2 cut(s) | 53, 64 |
| HphI | GGTGA | 1 cut(s) | 143 |
| Hpy188III | TCNNGA | 1 cut(s) | 340 |
| HpyAV | CCTTC | 1 cut(s) | 73 |
| HpyCH4V | TGCA | 7 cut(s) | 189, 209, 231, 251, 275, 297, 317 |
| HpyF3I | CTNAG | 1 cut(s) | 57 |
| Hsp92II | CATG | 4 cut(s) | 213, 255, 279, 321 |
| MluCI | AATT | 1 cut(s) | 178 |
| MlyI | GAGTC | 1 cut(s) | 58 |
| MnlI | CCTC | 1 cut(s) | 15 |
| MseI | TTAA | 1 cut(s) | 140 |
| MslI | CAYNNNNRTG | 4 cut(s) | 214, 256, 280, 322 |
| NlaIII | CATG | 4 cut(s) | 213, 255, 279, 321 |
| NspI | RCATGY | 4 cut(s) | 213, 255, 279, 321 |
| PfeI | GAWTC | 1 cut(s) | 53 |
| PleI | GAGTC | 1 cut(s) | 58 |
| PpsI | GAGTC | 1 cut(s) | 58 |
| RseI | CAYNNNNRTG | 4 cut(s) | 214, 256, 280, 322 |
| SaqAI | TTAA | 1 cut(s) | 140 |
| SchI | GAGTC | 1 cut(s) | 58 |
| SgeI | CNNG | 6 cut(s) | 80, 222, 264, 288, 330, 352 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 214, 256, 280, 322 |
| Sse9I | AATT | 1 cut(s) | 178 |
| TasI | AATT | 1 cut(s) | 178 |
| TfiI | GAWTC | 1 cut(s) | 53 |
| Tru1I | TTAA | 1 cut(s) | 140 |
| Tru9I | TTAA | 1 cut(s) | 140 |
| TspDTI | ATGAA | 3 cut(s) | 210, 252, 318 |
| XceI | RCATGY | 4 cut(s) | 213, 255, 279, 321 |