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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 891 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 444, 698 |
| AccI | GTMKAC | 1 cut(s) | 407 |
| AccII | CGCG | 1 cut(s) | 418 |
| AciI | CCGC | 2 cut(s) | 95, 1403 |
| AclWI | GGATC | 3 cut(s) | 1180, 1193, 1315 |
| AcsI | RAATTY | 5 cut(s) | 719, 1052, 1118, 1126, 1166 |
| AcuI | CTGAAG | 1 cut(s) | 997 |
| AfaI | GTAC | 4 cut(s) | 369, 740, 978, 1390 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 444, 698 |
| AflII | CTTAAG | 2 cut(s) | 535, 1112 |
| AgsI | TTSAA | 9 cut(s) | 259, 519, 882, 910, 1007, 1051, 1179, 1277, 1328 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 743, 775 |
| AluBI | AGCT | 4 cut(s) | 530, 540, 631, 1365 |
| AluI | AGCT | 4 cut(s) | 530, 540, 631, 1365 |
| Alw26I | GTCTC | 1 cut(s) | 860 |
| AlwI | GGATC | 3 cut(s) | 1180, 1193, 1315 |
| ApeKI | GCWGC | 1 cut(s) | 1362 |
| ApoI | RAATTY | 5 cut(s) | 719, 1052, 1118, 1126, 1166 |
| AseI | ATTAAT | 1 cut(s) | 591 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 722 |
| AspS9I | GGNCC | 2 cut(s) | 331, 747 |
| AsuHPI | GGTGA | 2 cut(s) | 109, 484 |
| AvaII | GGWCC | 2 cut(s) | 331, 747 |
| AxyI | CCTNAGG | 1 cut(s) | 157 |
| BamHI | GGATCC | 1 cut(s) | 1185 |
| BauI | CACGAG | 1 cut(s) | 775 |
| BbvI | GCAGC | 1 cut(s) | 1374 |
| BccI | CCATC | 4 cut(s) | 228, 251, 817, 1239 |
| BcoDI | GTCTC | 1 cut(s) | 860 |
| BfaI | CTAG | 3 cut(s) | 900, 939, 1182 |
| BfmI | CTRYAG | 1 cut(s) | 857 |
| BfrI | CTTAAG | 2 cut(s) | 535, 1112 |
| BisI | GCNGC | 1 cut(s) | 1363 |
| BlpI | GCTNAGC | 1 cut(s) | 1358 |
| BlsI | GCNGC | 1 cut(s) | 1364 |
| BmcAI | AGTACT | 1 cut(s) | 978 |
| Bme18I | GGWCC | 2 cut(s) | 331, 747 |
| BmgT120I | GGNCC | 2 cut(s) | 331, 747 |
| BmiI | GGNNCC | 1 cut(s) | 1187 |
| BmsI | GCATC | 2 cut(s) | 379, 1360 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1358 |
| BsaJI | CCNNGG | 1 cut(s) | 324 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 196, 226, 1027, 1057 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 444, 698 |
| Bse1I | ACTGG | 1 cut(s) | 5 |
| Bse21I | CCTNAGG | 1 cut(s) | 157 |
| BseDI | CCNNGG | 1 cut(s) | 324 |
| BseGI | GGATG | 5 cut(s) | 243, 394, 1204, 1323, 1349 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 444, 698 |
| BseNI | ACTGG | 1 cut(s) | 5 |
| BseRI | GAGGAG | 1 cut(s) | 204 |
| BseXI | GCAGC | 1 cut(s) | 1374 |
| Bsh1236I | CGCG | 1 cut(s) | 418 |
| BslFI | GGGAC | 1 cut(s) | 900 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 444, 698 |
| BsmAI | GTCTC | 1 cut(s) | 860 |
| BsmFI | GGGAC | 1 cut(s) | 900 |
| Bsp143I | GATC | 5 cut(s) | 787, 853, 1185, 1218, 1320 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1358 |
| BspACI | CCGC | 2 cut(s) | 95, 1403 |
| BspFNI | CGCG | 1 cut(s) | 418 |
| BspHI | TCATGA | 1 cut(s) | 1032 |
| BspLI | GGNNCC | 1 cut(s) | 1187 |
| BspMAI | CTGCAG | 1 cut(s) | 861 |
| BspPI | GGATC | 3 cut(s) | 1180, 1193, 1315 |
| BspTI | CTTAAG | 2 cut(s) | 535, 1112 |
| BsrI | ACTGG | 1 cut(s) | 5 |
| BssECI | CCNNGG | 1 cut(s) | 324 |
| BssMI | GATC | 5 cut(s) | 787, 853, 1185, 1218, 1320 |
| BssNAI | GTATAC | 1 cut(s) | 408 |
| BssSI | CACGAG | 1 cut(s) | 775 |
| BssT1I | CCWWGG | 1 cut(s) | 324 |
| Bst1107I | GTATAC | 1 cut(s) | 408 |
| Bst2BI | CACGAG | 1 cut(s) | 775 |
| Bst4CI | ACNGT | 2 cut(s) | 49, 1397 |
| Bst6I | CTCTTC | 3 cut(s) | 193, 470, 911 |
| BstAFI | CTTAAG | 2 cut(s) | 535, 1112 |
| BstDEI | CTNAG | 4 cut(s) | 157, 729, 984, 1358 |
| BstF5I | GGATG | 5 cut(s) | 243, 394, 1204, 1323, 1349 |
| BstFNI | CGCG | 1 cut(s) | 418 |
| BstKTI | GATC | 5 cut(s) | 790, 856, 1188, 1221, 1323 |
| BstMAI | GTCTC | 1 cut(s) | 860 |
| BstMBI | GATC | 5 cut(s) | 787, 853, 1185, 1218, 1320 |
| BstSFI | CTRYAG | 1 cut(s) | 857 |
| BstUI | CGCG | 1 cut(s) | 418 |
| BstV1I | GCAGC | 1 cut(s) | 1374 |
| BstX2I | RGATCY | 1 cut(s) | 1185 |
| BstYI | RGATCY | 1 cut(s) | 1185 |
| BstZ17I | GTATAC | 1 cut(s) | 408 |
| Bsu36I | CCTNAGG | 1 cut(s) | 157 |
| BtsCI | GGATG | 5 cut(s) | 243, 394, 1204, 1323, 1349 |
| BtsI | GCAGTG | 1 cut(s) | 302 |
| BtsIMutI | CAGTG | 1 cut(s) | 302 |
| CciI | TCATGA | 1 cut(s) | 1032 |
| Cfr13I | GGNCC | 2 cut(s) | 331, 747 |
| Csp6I | GTAC | 4 cut(s) | 368, 739, 977, 1389 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 50, 85 |
| CviAII | CATG | 5 cut(s) | 715, 1033, 1145, 1192, 1354 |
| CviJI | RGCY | 6 cut(s) | 238, 530, 540, 631, 1365, 1380 |
| CviKI_1 | RGCY | 6 cut(s) | 238, 530, 540, 631, 1365, 1380 |
| CviQI | GTAC | 4 cut(s) | 368, 739, 977, 1389 |
| DdeI | CTNAG | 4 cut(s) | 157, 729, 984, 1358 |
| DpnI | GATC | 5 cut(s) | 789, 855, 1187, 1220, 1322 |
| DpnII | GATC | 5 cut(s) | 787, 853, 1185, 1218, 1320 |
| DraI | TTTAAA | 1 cut(s) | 34 |
| Eam1104I | CTCTTC | 3 cut(s) | 193, 470, 911 |
| EarI | CTCTTC | 3 cut(s) | 193, 470, 911 |
| Eco130I | CCWWGG | 1 cut(s) | 324 |
| Eco32I | GATATC | 1 cut(s) | 659 |
| Eco47I | GGWCC | 2 cut(s) | 331, 747 |
| Eco57I | CTGAAG | 1 cut(s) | 997 |
| Eco81I | CCTNAGG | 1 cut(s) | 157 |
| EcoRV | GATATC | 1 cut(s) | 659 |
| EcoT14I | CCWWGG | 1 cut(s) | 324 |
| ErhI | CCWWGG | 1 cut(s) | 324 |
| FaeI | CATG | 5 cut(s) | 718, 1036, 1148, 1195, 1357 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 661, 693, 1095, 1127 |
| FaqI | GGGAC | 1 cut(s) | 900 |
| FatI | CATG | 5 cut(s) | 714, 1032, 1144, 1191, 1353 |
| FauNDI | CATATG | 4 cut(s) | 336, 379, 604, 694 |
| FblI | GTMKAC | 1 cut(s) | 407 |
| Fnu4HI | GCNGC | 1 cut(s) | 1363 |
| FokI | GGATG | 5 cut(s) | 230, 401, 1191, 1330, 1336 |
| Fsp4HI | GCNGC | 1 cut(s) | 1363 |
| FspBI | CTAG | 3 cut(s) | 900, 939, 1182 |
| GluI | GCNGC | 1 cut(s) | 1363 |
| Hin1II | CATG | 5 cut(s) | 718, 1036, 1148, 1195, 1357 |
| HincII | GTYRAC | 2 cut(s) | 991, 1345 |
| HindII | GTYRAC | 2 cut(s) | 991, 1345 |
| HinfI | GANTC | 4 cut(s) | 903, 1007, 1229, 1346 |
| HpaI | GTTAAC | 1 cut(s) | 991 |
| HphI | GGTGA | 2 cut(s) | 109, 484 |
| Hpy166II | GTNNAC | 5 cut(s) | 52, 408, 991, 1150, 1345 |
| Hpy188I | TCNGA | 3 cut(s) | 426, 654, 1125 |
| Hpy188III | TCNNGA | 6 cut(s) | 483, 745, 775, 865, 900, 1033 |
| Hpy8I | GTNNAC | 5 cut(s) | 52, 408, 991, 1150, 1345 |
| HpyAV | CCTTC | 2 cut(s) | 1021, 1334 |
| HpyCH4III | ACNGT | 2 cut(s) | 49, 1397 |
| HpyCH4IV | ACGT | 1 cut(s) | 194 |
| HpyCH4V | TGCA | 6 cut(s) | 435, 642, 859, 957, 1299, 1373 |
| HpyF3I | CTNAG | 4 cut(s) | 157, 729, 984, 1358 |
| HpySE526I | ACGT | 1 cut(s) | 194 |
| Hsp92II | CATG | 5 cut(s) | 718, 1036, 1148, 1195, 1357 |
| KspAI | GTTAAC | 1 cut(s) | 991 |
| Kzo9I | GATC | 5 cut(s) | 787, 853, 1185, 1218, 1320 |
| LmnI | GCTCC | 1 cut(s) | 397 |
| LpnPI | CCDG | 6 cut(s) | 18, 411, 468, 730, 1157, 1218 |
| Lsp1109I | GCAGC | 1 cut(s) | 1374 |
| LweI | GCATC | 2 cut(s) | 379, 1360 |
| MaeI | CTAG | 3 cut(s) | 900, 939, 1182 |
| MaeII | ACGT | 1 cut(s) | 194 |
| MaeIII | GTNAC | 2 cut(s) | 553, 1253 |
| MalI | GATC | 5 cut(s) | 789, 855, 1187, 1220, 1322 |
| MboI | GATC | 5 cut(s) | 787, 853, 1185, 1218, 1320 |
| MboII | GAAGA | 9 cut(s) | 180, 201, 243, 487, 498, 560, 793, 898, 1115 |
| MflI | RGATCY | 1 cut(s) | 1185 |
| MlyI | GAGTC | 3 cut(s) | 897, 1238, 1340 |
| MmeI | TCCRAC | 1 cut(s) | 477 |
| MroXI | GAANNNNTTC | 1 cut(s) | 722 |
| MseI | TTAA | 9 cut(s) | 33, 288, 359, 512, 536, 591, 990, 1113, 1163 |
| MslI | CAYNNNNRTG | 2 cut(s) | 378, 821 |
| MspCI | CTTAAG | 2 cut(s) | 535, 1112 |
| MvnI | CGCG | 1 cut(s) | 418 |
| NdeI | CATATG | 4 cut(s) | 336, 379, 604, 694 |
| NdeII | GATC | 5 cut(s) | 787, 853, 1185, 1218, 1320 |
| NlaIII | CATG | 5 cut(s) | 718, 1036, 1148, 1195, 1357 |
| NlaIV | GGNNCC | 1 cut(s) | 1187 |
| NmuCI | GTSAC | 1 cut(s) | 1253 |
| PagI | TCATGA | 1 cut(s) | 1032 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 422 |
| PdmI | GAANNNNTTC | 1 cut(s) | 722 |
| PfeI | GAWTC | 1 cut(s) | 1007 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 444, 698 |
| PkrI | GCNGC | 1 cut(s) | 1364 |
| PleI | GAGTC | 3 cut(s) | 897, 1237, 1340 |
| PpsI | GAGTC | 3 cut(s) | 897, 1237, 1340 |
| PshBI | ATTAAT | 1 cut(s) | 591 |
| PsiI | TTATAA | 1 cut(s) | 891 |
| PspN4I | GGNNCC | 1 cut(s) | 1187 |
| PspPI | GGNCC | 2 cut(s) | 331, 747 |
| PstI | CTGCAG | 1 cut(s) | 861 |
| PsuI | RGATCY | 1 cut(s) | 1185 |
| RsaI | GTAC | 4 cut(s) | 369, 740, 978, 1390 |
| RsaNI | GTAC | 4 cut(s) | 368, 739, 977, 1389 |
| RseI | CAYNNNNRTG | 2 cut(s) | 378, 821 |
| SaqAI | TTAA | 9 cut(s) | 33, 288, 359, 512, 536, 591, 990, 1113, 1163 |
| SatI | GCNGC | 1 cut(s) | 1363 |
| Sau3AI | GATC | 5 cut(s) | 787, 853, 1185, 1218, 1320 |
| Sau96I | GGNCC | 2 cut(s) | 331, 747 |
| ScaI | AGTACT | 1 cut(s) | 978 |
| SchI | GAGTC | 3 cut(s) | 897, 1238, 1340 |
| SfaNI | GCATC | 2 cut(s) | 379, 1360 |
| SfcI | CTRYAG | 1 cut(s) | 857 |
| SinI | GGWCC | 2 cut(s) | 331, 747 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 378, 821 |
| SmlI | CTYRAG | 2 cut(s) | 535, 1112 |
| SmoI | CTYRAG | 2 cut(s) | 535, 1112 |
| SsiI | CCGC | 2 cut(s) | 95, 1403 |
| SspI | AATATT | 4 cut(s) | 125, 286, 345, 873 |
| SspMI | CTAG | 3 cut(s) | 900, 939, 1182 |
| StyI | CCWWGG | 1 cut(s) | 324 |
| TaaI | ACNGT | 2 cut(s) | 49, 1397 |
| TaiI | ACGT | 1 cut(s) | 197 |
| TaqI | TCGA | 4 cut(s) | 852, 864, 1243, 1368 |
| TatI | WGTACW | 2 cut(s) | 367, 976 |
| TfiI | GAWTC | 1 cut(s) | 1007 |
| Tru1I | TTAA | 9 cut(s) | 33, 288, 359, 512, 536, 591, 990, 1113, 1163 |
| Tru9I | TTAA | 9 cut(s) | 33, 288, 359, 512, 536, 591, 990, 1113, 1163 |
| TscAI | CASTG | 1 cut(s) | 309 |
| TseFI | GTSAC | 1 cut(s) | 1253 |
| TseI | GCWGC | 1 cut(s) | 1362 |
| Tsp45I | GTSAC | 1 cut(s) | 1253 |
| TspDTI | ATGAA | 9 cut(s) | 10, 243, 562, 621, 669, 731, 751, 1021, 1116 |
| TspGWI | ACGGA | 1 cut(s) | 517 |
| TspRI | CASTG | 1 cut(s) | 309 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 444, 698 |
| Vha464I | CTTAAG | 2 cut(s) | 535, 1112 |
| VpaK11BI | GGWCC | 2 cut(s) | 331, 747 |
| VspI | ATTAAT | 1 cut(s) | 591 |
| XapI | RAATTY | 5 cut(s) | 719, 1052, 1118, 1126, 1166 |
| XbaI | TCTAGA | 1 cut(s) | 899 |
| XmiI | GTMKAC | 1 cut(s) | 407 |
| XmnI | GAANNNNTTC | 1 cut(s) | 722 |
| XspI | CTAG | 3 cut(s) | 900, 939, 1182 |
| ZrmI | AGTACT | 1 cut(s) | 978 |