MD05G1217100.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000201
Physical Location & Seq
Forward (+)
34780202 .. 34781645
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1217100.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGATTGCAGACAAGAATACGTTGGAAAAGATAGGGAAGAGATGAAGTTACCATTGTTCGACTTGACCACCATAGCTAAAGCCACTAACAACTTTTCAAGCTACAACAAGCTGGGAGAAGGTGGCTTTGGACCTGTGTCCAAGGGTACATTGATAGGAGGAAACGTAATTGCAGTAAAGAGGCTTTCAAAGAATTCTGGACAAGGAATAAGAGAGTTCAGAACTGAACCTAAGTGCTTCCACATTATCGGTGGAGTTGCCCGAGGGCTTCTTTATCTTCACCAAGACTCTAGATTAAGGATTATACATCGAGATCTGAAACCTAGCAATATTCTGCTAGATGATAATATGAACCCGAAAATTTCAGACTTCGGCCTGGCTAAAACATTTGGCGGTGAACAAAGTCAGGCCAACACAAATAAAGTGGTTGGAACCTATGGTTATATGTCTCCGGAATATGCAACAGATGGAATTTTTTCGATGAAATCTGATGTGTTTAGCTTTGGAGTCATACTGCTCGAGATGTTGAGTAGGAAGAAGAACAGGGGATTTCGTCATCTAGATCACGACCACAACCTTCTTGGACATACGTTTTGTGGTTCGTGCAACACATCTGAAGTGGTAAGGTGTCTTCATGTGTGGCTGTTATGTGCGCAAAGAGTACCTGAGGATAGACCAAGCATGTCATCTGTGGTTCTGATGTTGAGCAGTGATGTTGCCTTGCCGCCTCCAAAGCAGCCCGGTTTTTACACTGAACAAAGTGTCCCTAAATCACCATCAAGGACGCTTCTTTGTTCAGAGAATACTTTCAGCACTACGTTGGTAGAACCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

279

Amino Acids

30.92

Weight (kDa)

8.92

Isoelectric Point (pI)

53.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 75 - 180 5.8e-28 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 77 - 233 8.1e-25 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 236 - 278 5.4e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 654
AccIII TCCGGA 1 cut(s) 451
AciI CCGC 2 cut(s) 393, 725
AcsI RAATTY 3 cut(s) 193, 360, 471
AcuI CTGAAG 1 cut(s) 636
AfaI GTAC 2 cut(s) 148, 663
AgsI TTSAA 2 cut(s) 99, 189
AjnI CCWGG 1 cut(s) 375
AjuI GAANNNNNNNTTGG 2 cut(s) 111, 143
AloI GAACNNNNNNTCC 2 cut(s) 747, 779
AluBI AGCT 4 cut(s) 77, 102, 112, 501
AluI AGCT 4 cut(s) 77, 102, 112, 501
Alw26I GTCTC 1 cut(s) 453
Ama87I CYCGRG 2 cut(s) 261, 518
Aor13HI TCCGGA 1 cut(s) 451
AoxI GGCC 2 cut(s) 373, 408
ApeKI GCWGC 1 cut(s) 736
ApoI RAATTY 3 cut(s) 193, 360, 471
ArsI GACNNNNNNTTYG 2 cut(s) 576, 608
Asp700I GAANNNNTTC 2 cut(s) 475, 806
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 1 cut(s) 131
AsuC2I CCSGG 1 cut(s) 741
AsuHPI GGTGA 3 cut(s) 272, 407, 765
AvaI CYCGRG 2 cut(s) 261, 518
AvaII GGWCC 1 cut(s) 131
AxyI CCTNAGG 1 cut(s) 666
BarI GAAGNNNNNNTAC 2 cut(s) 615, 647
BbsI GAAGAC 1 cut(s) 623
BbvI GCAGC 1 cut(s) 748
BccI CCATC 2 cut(s) 461, 784
BciT130I CCWGG 1 cut(s) 377
BcnI CCSGG 1 cut(s) 741
BcoDI GTCTC 1 cut(s) 453
BfaI CTAG 4 cut(s) 291, 324, 338, 560
BglII AGATCT 1 cut(s) 313
BisI GCNGC 2 cut(s) 725, 737
BlsI GCNGC 2 cut(s) 726, 738
Bme1390I CCNGG 2 cut(s) 377, 741
Bme18I GGWCC 1 cut(s) 131
BmeT110I CYCGRG 2 cut(s) 261, 518
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 2 cut(s) 377, 741
BoxI GACNNNNGTC 1 cut(s) 136
BpiI GAAGAC 1 cut(s) 623
BpuMI CCSGG 1 cut(s) 741
BsaJI CCNNGG 3 cut(s) 141, 262, 830
BsaWI WCCGGW 1 cut(s) 451
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bse21I CCTNAGG 1 cut(s) 666
BseAI TCCGGA 1 cut(s) 451
BseBI CCWGG 1 cut(s) 377
BseDI CCNNGG 3 cut(s) 141, 262, 830
BseMII CTCAG 1 cut(s) 657
BseXI GCAGC 1 cut(s) 748
BseYI CCCAGC 1 cut(s) 112
BshFI GGCC 2 cut(s) 375, 410
BsiHKCI CYCGRG 2 cut(s) 261, 518
BsiSI CCGG 2 cut(s) 452, 741
BslFI GGGAC 1 cut(s) 749
BsmAI GTCTC 1 cut(s) 453
BsmFI GGGAC 1 cut(s) 749
BsnI GGCC 2 cut(s) 375, 410
BsoBI CYCGRG 2 cut(s) 261, 518
Bsp13I TCCGGA 1 cut(s) 451
Bsp143I GATC 2 cut(s) 313, 562
BspACI CCGC 2 cut(s) 393, 725
BspANI GGCC 2 cut(s) 375, 410
BspCNI CTCAG 1 cut(s) 658
BspEI TCCGGA 1 cut(s) 451
BspLI GGNNCC 1 cut(s) 433
BssECI CCNNGG 3 cut(s) 141, 262, 830
BssMI GATC 2 cut(s) 313, 562
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 377
Bst6I CTCTTC 1 cut(s) 33
BstDEI CTNAG 2 cut(s) 231, 666
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 2 cut(s) 316, 565
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 2 cut(s) 313, 562
BstMWI GCNNNNNNNGC 1 cut(s) 733
BstNI CCWGG 1 cut(s) 377
BstNSI RCATGY 1 cut(s) 685
BstPAI GACNNNNGTC 1 cut(s) 136
BstSCI CCNGG 2 cut(s) 375, 739
BstV1I GCAGC 1 cut(s) 748
BstV2I GAAGAC 1 cut(s) 623
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
Bsu36I CCTNAGG 1 cut(s) 666
BsuRI GGCC 2 cut(s) 375, 410
BtsI GCAGTG 1 cut(s) 715
BtsIMutI CAGTG 2 cut(s) 715, 750
CfoI GCGC 1 cut(s) 655
Cfr13I GGNCC 1 cut(s) 131
CseI GACGC 1 cut(s) 793
Csp6I GTAC 2 cut(s) 147, 662
CspCI CAANNNNNGTGG 2 cut(s) 405, 440
CviAII CATG 2 cut(s) 635, 682
CviQI GTAC 2 cut(s) 147, 662
DdeI CTNAG 2 cut(s) 231, 666
DpnI GATC 2 cut(s) 315, 564
DpnII GATC 2 cut(s) 313, 562
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 636
Eco81I CCTNAGG 1 cut(s) 666
Eco88I CYCGRG 2 cut(s) 261, 518
EcoRI GAATTC 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 2 cut(s) 638, 685
FaqI GGGAC 1 cut(s) 749
FatI CATG 2 cut(s) 634, 681
Fnu4HI GCNGC 2 cut(s) 725, 737
Fsp4HI GCNGC 2 cut(s) 725, 737
FspBI CTAG 4 cut(s) 291, 324, 338, 560
FspI TGCGCA 1 cut(s) 654
GlaI GCGC 1 cut(s) 654
GluI GCNGC 2 cut(s) 725, 737
GsaI CCCAGC 1 cut(s) 116
HaeIII GGCC 2 cut(s) 375, 410
HapII CCGG 2 cut(s) 452, 741
HgaI GACGC 1 cut(s) 793
HhaI GCGC 1 cut(s) 655
Hin1II CATG 2 cut(s) 638, 685
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HinfI GANTC 2 cut(s) 287, 507
HpaII CCGG 2 cut(s) 452, 741
HphI GGTGA 3 cut(s) 272, 407, 765
Hpy166II GTNNAC 1 cut(s) 398
Hpy188I TCNGA 7 cut(s) 221, 318, 367, 490, 616, 699, 799
Hpy188III TCNNGA 7 cut(s) 198, 291, 311, 452, 520, 560, 566
Hpy8I GTNNAC 1 cut(s) 398
HpyAV CCTTC 2 cut(s) 113, 587
HpyCH4IV ACGT 4 cut(s) 21, 165, 590, 818
HpyCH4V TGCA 4 cut(s) 9, 173, 461, 606
HpyF10VI GCNNNNNNNGC 1 cut(s) 733
HpyF3I CTNAG 2 cut(s) 231, 666
HpySE526I ACGT 4 cut(s) 21, 165, 590, 818
Hsp92II CATG 2 cut(s) 638, 685
HspAI GCGC 1 cut(s) 653
Kpn2I TCCGGA 1 cut(s) 451
Kzo9I GATC 2 cut(s) 313, 562
Lsp1109I GCAGC 1 cut(s) 748
MaeI CTAG 4 cut(s) 291, 324, 338, 560
MaeII ACGT 4 cut(s) 21, 165, 590, 818
MaeIII GTNAC 1 cut(s) 48
MalI GATC 2 cut(s) 315, 564
MboI GATC 2 cut(s) 313, 562
MboII GAAGA 5 cut(s) 50, 269, 547, 550, 623
MflI RGATCY 1 cut(s) 313
MluCI AATT 4 cut(s) 168, 193, 360, 471
MlyI GAGTC 2 cut(s) 281, 516
MmeI TCCRAC 2 cut(s) 4, 409
MnlI CCTC 5 cut(s) 152, 174, 257, 661, 738
MroI TCCGGA 1 cut(s) 451
MroXI GAANNNNTTC 2 cut(s) 475, 806
MseI TTAA 1 cut(s) 296
MspI CCGG 2 cut(s) 452, 741
MspR9I CCNGG 2 cut(s) 377, 741
MvaI CCWGG 1 cut(s) 377
MwoI GCNNNNNNNGC 1 cut(s) 733
NciI CCSGG 1 cut(s) 741
NdeII GATC 2 cut(s) 313, 562
NlaIII CATG 2 cut(s) 638, 685
NlaIV GGNNCC 1 cut(s) 433
NsbI TGCGCA 1 cut(s) 654
NspI RCATGY 1 cut(s) 685
PaeR7I CTCGAG 1 cut(s) 518
PdmI GAANNNNTTC 2 cut(s) 475, 806
PkrI GCNGC 2 cut(s) 726, 738
PleI GAGTC 2 cut(s) 281, 515
PpsI GAGTC 2 cut(s) 281, 515
PshAI GACNNNNGTC 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 375
PspFI CCCAGC 1 cut(s) 112
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 433
PspPI GGNCC 1 cut(s) 131
PsuI RGATCY 1 cut(s) 313
RsaI GTAC 2 cut(s) 148, 663
RsaNI GTAC 2 cut(s) 147, 662
SaqAI TTAA 1 cut(s) 296
SatI GCNGC 2 cut(s) 725, 737
Sau3AI GATC 2 cut(s) 313, 562
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 2 cut(s) 281, 516
ScrFI CCNGG 2 cut(s) 377, 741
Sfr274I CTCGAG 1 cut(s) 518
SinI GGWCC 1 cut(s) 131
SlaI CTCGAG 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
Sse9I AATT 4 cut(s) 168, 193, 360, 471
SsiI CCGC 2 cut(s) 393, 725
SspI AATATT 1 cut(s) 331
SspMI CTAG 4 cut(s) 291, 324, 338, 560
StyD4I CCNGG 2 cut(s) 375, 739
StyI CCWWGG 1 cut(s) 141
TaiI ACGT 4 cut(s) 24, 168, 593, 821
TaqI TCGA 4 cut(s) 60, 310, 479, 519
TasI AATT 4 cut(s) 168, 193, 360, 471
TauI GCSGC 1 cut(s) 727
Tru1I TTAA 1 cut(s) 296
Tru9I TTAA 1 cut(s) 296
TscAI CASTG 2 cut(s) 715, 757
TseI GCWGC 1 cut(s) 736
TspDTI ATGAA 4 cut(s) 59, 365, 497, 623
TspRI CASTG 2 cut(s) 715, 757
VpaK11BI GGWCC 1 cut(s) 131
XapI RAATTY 3 cut(s) 193, 360, 471
XbaI TCTAGA 2 cut(s) 290, 559
XceI RCATGY 1 cut(s) 685
XcmI CCANNNNNNNNNTGG 2 cut(s) 248, 578
XhoI CTCGAG 1 cut(s) 518
XmnI GAANNNNTTC 2 cut(s) 475, 806
XspI CTAG 4 cut(s) 291, 324, 338, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.