Rh5AG189100

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000201
Physical Location & Seq
Reverse (-)
21766426 .. 21767997
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG189100.1

Sequence Viewer

Length: 306 bp
ATGGCATGGACACTATGGACACAAAATGTACCACTGGAACTCATCGATAAGACATTATGTGATCCGGATACCATATCCGAAGTTTTAAGGTGTCTTCATGTGGGTCTGTTATGTGTGCAGCAAGTACCTGAAGATAGACCAAGCATGTCATCTGTGGTACTAATGTTGGGCAGTCATGATGTTGTGCTGCCTTTGCCAAAGCAGCCTGGTTTTTACACTGAACGGACTCTTCCCGAATCATCATCAAGGTCACGTGACCTGTGTTCACCCAGTAACTTCAGCACTACATTGTTACAGGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

101

Amino Acids

11.3

Weight (kDa)

5.16

Isoelectric Point (pI)

68.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3403 PF11883 59 - 101 1.5e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 64
AclWI GGATC 1 cut(s) 56
AcuI CTGAAG 2 cut(s) 150, 262
AcvI CACGTG 1 cut(s) 254
AfaI GTAC 3 cut(s) 30, 126, 159
AjnI CCWGG 1 cut(s) 205
AlwI GGATC 1 cut(s) 56
Aor13HI TCCGGA 1 cut(s) 64
ApeKI GCWGC 3 cut(s) 118, 187, 202
AsuHPI GGTGA 1 cut(s) 258
BaeI ACNNNNGTAYC 2 cut(s) 149, 182
BbrPI CACGTG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 86
BbvI GCAGC 3 cut(s) 130, 174, 214
BciT130I CCWGG 1 cut(s) 207
BciVI GTATCC 1 cut(s) 61
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 3 cut(s) 119, 188, 203
BlsI GCNGC 3 cut(s) 120, 189, 204
Bme1390I CCNGG 1 cut(s) 207
BmrFI CCNGG 1 cut(s) 207
BmrI ACTGGG 1 cut(s) 264
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 86
Bsa29I ATCGAT 1 cut(s) 45
BsaAI YACGTR 1 cut(s) 254
BsaWI WCCGGW 1 cut(s) 64
Bse1I ACTGG 2 cut(s) 39, 270
BseAI TCCGGA 1 cut(s) 64
BseBI CCWGG 1 cut(s) 207
BseCI ATCGAT 1 cut(s) 45
BseNI ACTGG 2 cut(s) 39, 270
BseXI GCAGC 3 cut(s) 130, 174, 214
BsgI GTGCAG 1 cut(s) 137
BshVI ATCGAT 1 cut(s) 45
BsiSI CCGG 1 cut(s) 65
Bsp13I TCCGGA 1 cut(s) 64
Bsp143I GATC 1 cut(s) 61
BspDI ATCGAT 1 cut(s) 45
BspEI TCCGGA 1 cut(s) 64
BspHI TCATGA 1 cut(s) 175
BspPI GGATC 1 cut(s) 56
BsrI ACTGG 2 cut(s) 39, 270
BssMI GATC 1 cut(s) 61
Bst2UI CCWGG 1 cut(s) 207
Bst6I CTCTTC 1 cut(s) 234
BstBAI YACGTR 1 cut(s) 254
BstKTI GATC 1 cut(s) 64
BstMBI GATC 1 cut(s) 61
BstMWI GCNNNNNNNGC 2 cut(s) 193, 202
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 205
BstV1I GCAGC 3 cut(s) 130, 174, 214
BstV2I GAAGAC 1 cut(s) 86
Bsu15I ATCGAT 1 cut(s) 45
BsuI GTATCC 1 cut(s) 61
BsuTUI ATCGAT 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 32, 216
CciI TCATGA 1 cut(s) 175
ClaI ATCGAT 1 cut(s) 45
Csp6I GTAC 3 cut(s) 29, 125, 158
CviAII CATG 4 cut(s) 6, 98, 145, 176
CviJI RGCY 2 cut(s) 205, 299
CviKI_1 RGCY 2 cut(s) 205, 299
CviQI GTAC 3 cut(s) 29, 125, 158
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco57I CTGAAG 2 cut(s) 150, 262
Eco72I CACGTG 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 205
FaeI CATG 4 cut(s) 9, 101, 148, 179
FaiI YATR 8 cut(s) 7, 16, 58, 74, 99, 112, 146, 177
FatI CATG 4 cut(s) 5, 97, 144, 175
Fnu4HI GCNGC 3 cut(s) 119, 188, 203
Fsp4HI GCNGC 3 cut(s) 119, 188, 203
GluI GCNGC 3 cut(s) 119, 188, 203
HapII CCGG 1 cut(s) 65
Hin1II CATG 4 cut(s) 9, 101, 148, 179
HinfI GANTC 2 cut(s) 226, 236
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 258
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 1 cut(s) 79
Hpy188III TCNNGA 3 cut(s) 65, 176, 233
Hpy8I GTNNAC 1 cut(s) 266
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 1 cut(s) 118
HpyF10VI GCNNNNNNNGC 2 cut(s) 193, 202
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 4 cut(s) 9, 101, 148, 179
Kpn2I TCCGGA 1 cut(s) 64
Kzo9I GATC 1 cut(s) 61
LpnPI CCDG 8 cut(s) 20, 78, 141, 192, 219, 272, 281, 283
Lsp1109I GCAGC 3 cut(s) 130, 174, 214
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 4 cut(s) 249, 254, 272, 291
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 3 cut(s) 86, 143, 221
MlyI GAGTC 1 cut(s) 220
MroI TCCGGA 1 cut(s) 64
MseI TTAA 1 cut(s) 86
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 207
MwoI GCNNNNNNNGC 2 cut(s) 193, 202
NdeII GATC 1 cut(s) 61
NlaIII CATG 4 cut(s) 9, 101, 148, 179
NmuCI GTSAC 2 cut(s) 249, 254
NspI RCATGY 1 cut(s) 148
PagI TCATGA 1 cut(s) 175
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 3 cut(s) 120, 189, 204
PleI GAGTC 1 cut(s) 220
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
PpsI GAGTC 1 cut(s) 220
Ppu21I YACGTR 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 205
PspCI CACGTG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 205
RsaI GTAC 3 cut(s) 30, 126, 159
RsaNI GTAC 3 cut(s) 29, 125, 158
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 3 cut(s) 119, 188, 203
Sau3AI GATC 1 cut(s) 61
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 1 cut(s) 207
SetI ASST 5 cut(s) 92, 130, 251, 256, 261
StyD4I CCNGG 1 cut(s) 205
TaiI ACGT 1 cut(s) 256
TaqI TCGA 1 cut(s) 45
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TscAI CASTG 2 cut(s) 39, 223
TseFI GTSAC 2 cut(s) 249, 254
TseI GCWGC 3 cut(s) 118, 187, 202
Tsp45I GTSAC 2 cut(s) 249, 254
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 1 cut(s) 238
TspRI CASTG 2 cut(s) 39, 223
XceI RCATGY 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.