RchiOBHm_Chr5g0036451

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000201
Physical Location & Seq
Forward (+)
30649703 .. 30650907
1205 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31526

Sequence Viewer

Length: 732 bp
ATGCCCAATAGAAGCTTGGACTACTTCATATTTGGTGATACAAGAAGTAAATTGCTTGGTTGGGATCAACGCATCAACATTATTGGTGGAATAGCTCGAGGCCTTCTCTATCTTCATCAAGATTCTAGACTGAGGATCATCCATAGAGATCTTAAAACTGGTAATGTTTTACTAGATAAAGATATGAACCCAAAAATATCTGATTTTGGGACGGCTAGAGCATTCGTAGGAGATCAAACTGCGGAAAATACTAAGACCGTGGTTGGGACATATGGTTATATGTCTCCTGAGTATGTGGTTGATGGACTCTTCTCAATCAAATCTGATGTCTATAGTTTCGGTGTCATGGTATTGGAGATAGTGAGTGGGAAGAAGAACAGGGGATTCATTCATCCAGACCACCAACTTAACCTTATAGGACATGTATGGACTCTATGGACTGAAGGAAGATCACTGGAAGTAATGGATAAGAAGTTGGATGGCTCCTATGCTCTAACAGAAGTGTCACGATGCATTCACATAGCTCTGTTGTGTGTGCAACAACAACCAGAAGATAGACCAAACATGGCATCTGTGGTCCTAATGTTGGGCGGTGAGGGATCATTGCCTGTGCCAAAGCAACCTGGTTTCTTCACTGAAAGGAATCCGTTTGAAGCAGAGAACTCTTCATCAAGCCAATTGGAATCATATTCAGTAAATGACATGAGTATAACAGAGTTAGAGGCACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

243

Amino Acids

27.25

Weight (kDa)

5.7

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 192 2.2e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 16 - 194 2.2e-26 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 200 - 243 3.8e-13 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 242, 591
AclWI GGATC 3 cut(s) 72, 143, 607
AcuI CTGAAG 1 cut(s) 462
AfiI CCNNNNNNNGG 2 cut(s) 264, 586
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 1 cut(s) 653
AjnI CCWGG 1 cut(s) 622
AluBI AGCT 3 cut(s) 15, 95, 524
AluI AGCT 3 cut(s) 15, 95, 524
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 3 cut(s) 72, 143, 607
Ama87I CYCGRG 1 cut(s) 96
AoxI GGCC 1 cut(s) 100
ArsI GACNNNNNNTTYG 2 cut(s) 312, 344
AspS9I GGNCC 1 cut(s) 577
AsuHPI GGTGA 2 cut(s) 47, 605
AvaI CYCGRG 1 cut(s) 96
AvaII GGWCC 1 cut(s) 577
BccI CCATC 2 cut(s) 296, 473
BceAI ACGGC 1 cut(s) 228
BciT130I CCWGG 1 cut(s) 624
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 3 cut(s) 126, 173, 216
BfmI CTRYAG 1 cut(s) 331
BglII AGATCT 1 cut(s) 148
Bme1390I CCNGG 1 cut(s) 624
Bme18I GGWCC 1 cut(s) 577
BmeT110I CYCGRG 1 cut(s) 96
BmgT120I GGNCC 1 cut(s) 577
BmiI GGNNCC 1 cut(s) 484
BmrFI CCNGG 1 cut(s) 624
BmsI GCATC 3 cut(s) 81, 500, 578
BplI GAGNNNNNCTC 2 cut(s) 90, 122
BsaJI CCNNGG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 2 cut(s) 264, 586
Bse1I ACTGG 2 cut(s) 163, 459
Bse3DI GCAATG 1 cut(s) 602
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 3 cut(s) 138, 391, 484
BseLI CCNNNNNNNGG 2 cut(s) 264, 586
BseMI GCAATG 1 cut(s) 602
BseMII CTCAG 2 cut(s) 122, 279
BseNI ACTGG 2 cut(s) 163, 459
BshFI GGCC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 96
BslFI GGGAC 2 cut(s) 223, 280
BslI CCNNNNNNNGG 2 cut(s) 264, 586
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 2 cut(s) 223, 280
BsmI GAATGC 2 cut(s) 221, 513
BsnI GGCC 1 cut(s) 102
BsoBI CYCGRG 1 cut(s) 96
Bsp143I GATC 6 cut(s) 64, 135, 148, 232, 449, 599
BspACI CCGC 2 cut(s) 242, 591
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 2 cut(s) 123, 280
BspLI GGNNCC 1 cut(s) 484
BspPI GGATC 3 cut(s) 72, 143, 607
BsrDI GCAATG 1 cut(s) 602
BsrI ACTGG 2 cut(s) 163, 459
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 6 cut(s) 64, 135, 148, 232, 449, 599
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 2 cut(s) 314, 670
BstDEI CTNAG 3 cut(s) 131, 252, 288
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 3 cut(s) 138, 391, 484
BstKTI GATC 6 cut(s) 67, 138, 151, 235, 452, 602
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 6 cut(s) 64, 135, 148, 232, 449, 599
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 622
BstSFI CTRYAG 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
BsuRI GGCC 1 cut(s) 102
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 3 cut(s) 138, 391, 484
BtsIMutI CAGTG 2 cut(s) 452, 633
Cfr13I GGNCC 1 cut(s) 577
CsiI ACCWGGT 1 cut(s) 622
CviAII CATG 4 cut(s) 346, 422, 565, 703
CviJI RGCY 7 cut(s) 15, 95, 102, 215, 483, 524, 675
CviKI_1 RGCY 7 cut(s) 15, 95, 102, 215, 483, 524, 675
DdeI CTNAG 3 cut(s) 131, 252, 288
DpnI GATC 6 cut(s) 66, 137, 150, 234, 451, 601
DpnII GATC 6 cut(s) 64, 135, 148, 232, 449, 599
Eam1104I CTCTTC 2 cut(s) 314, 670
EarI CTCTTC 2 cut(s) 314, 670
Eco147I AGGCCT 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 577
Eco57I CTGAAG 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 622
EcoT22I ATGCAT 1 cut(s) 515
FaeI CATG 4 cut(s) 349, 425, 568, 706
FaqI GGGAC 2 cut(s) 223, 280
FatI CATG 4 cut(s) 345, 421, 564, 702
FauNDI CATATG 1 cut(s) 271
FokI GGATG 3 cut(s) 125, 378, 491
FspBI CTAG 3 cut(s) 126, 173, 216
HaeIII GGCC 1 cut(s) 102
Hin1II CATG 4 cut(s) 349, 425, 568, 706
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 6 cut(s) 122, 306, 384, 430, 643, 683
HphI GGTGA 2 cut(s) 47, 605
Hpy188I TCNGA 2 cut(s) 202, 325
Hpy188III TCNNGA 5 cut(s) 119, 126, 287, 395, 507
HpyAV CCTTC 2 cut(s) 113, 437
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4V TGCA 2 cut(s) 513, 538
HpyF3I CTNAG 3 cut(s) 131, 252, 288
Hsp92II CATG 4 cut(s) 349, 425, 568, 706
Kzo9I GATC 6 cut(s) 64, 135, 148, 232, 449, 599
LmnI GCTCC 1 cut(s) 488
LpnPI CCDG 9 cut(s) 144, 300, 364, 408, 440, 561, 609, 621, 636
LweI GCATC 3 cut(s) 81, 500, 578
MabI ACCWGGT 1 cut(s) 622
MaeI CTAG 3 cut(s) 126, 173, 216
MaeIII GTNAC 1 cut(s) 504
MalI GATC 6 cut(s) 66, 137, 150, 234, 451, 601
MboI GATC 6 cut(s) 64, 135, 148, 232, 449, 599
MboII GAAGA 8 cut(s) 104, 301, 382, 385, 459, 563, 622, 657
MfeI CAATTG 1 cut(s) 677
MflI RGATCY 1 cut(s) 148
MluCI AATT 2 cut(s) 50, 677
MlyI GAGTC 2 cut(s) 300, 424
MmeI TCCRAC 1 cut(s) 456
MnlI CCTC 4 cut(s) 92, 126, 589, 715
Mph1103I ATGCAT 1 cut(s) 515
MseI TTAA 2 cut(s) 153, 408
MspR9I CCNGG 1 cut(s) 624
MunI CAATTG 1 cut(s) 677
Mva1269I GAATGC 2 cut(s) 221, 513
MvaI CCWGG 1 cut(s) 624
NdeI CATATG 1 cut(s) 271
NdeII GATC 6 cut(s) 64, 135, 148, 232, 449, 599
NlaIII CATG 4 cut(s) 349, 425, 568, 706
NlaIV GGNNCC 1 cut(s) 484
NmuCI GTSAC 1 cut(s) 504
NsiI ATGCAT 1 cut(s) 515
NspI RCATGY 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 96
PceI AGGCCT 1 cut(s) 102
PciI ACATGT 1 cut(s) 421
PctI GAATGC 2 cut(s) 221, 513
PfeI GAWTC 4 cut(s) 122, 384, 643, 683
PleI GAGTC 2 cut(s) 300, 424
PpsI GAGTC 2 cut(s) 300, 424
PscI ACATGT 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 1 cut(s) 577
PspXI VCTCGAGB 1 cut(s) 96
PsuI RGATCY 1 cut(s) 148
SaqAI TTAA 2 cut(s) 153, 408
Sau3AI GATC 6 cut(s) 64, 135, 148, 232, 449, 599
Sau96I GGNCC 1 cut(s) 577
SchI GAGTC 2 cut(s) 300, 424
ScrFI CCNGG 1 cut(s) 624
SetI ASST 5 cut(s) 17, 97, 414, 526, 625
SexAI ACCWGGT 1 cut(s) 622
SfaNI GCATC 3 cut(s) 81, 500, 578
SfcI CTRYAG 1 cut(s) 331
Sfr274I CTCGAG 1 cut(s) 96
SinI GGWCC 1 cut(s) 577
SlaI CTCGAG 1 cut(s) 96
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
Sse9I AATT 2 cut(s) 50, 677
SseBI AGGCCT 1 cut(s) 102
SsiI CCGC 2 cut(s) 242, 591
SspMI CTAG 3 cut(s) 126, 173, 216
StuI AGGCCT 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 622
TaaI ACNGT 1 cut(s) 259
TaqI TCGA 1 cut(s) 97
TasI AATT 2 cut(s) 50, 677
TfiI GAWTC 4 cut(s) 122, 384, 643, 683
Tru1I TTAA 2 cut(s) 153, 408
Tru9I TTAA 2 cut(s) 153, 408
TscAI CASTG 2 cut(s) 459, 640
TseFI GTSAC 1 cut(s) 504
Tsp45I GTSAC 1 cut(s) 504
TspDTI ATGAA 6 cut(s) 16, 104, 200, 376, 380, 657
TspGWI ACGGA 1 cut(s) 636
TspRI CASTG 2 cut(s) 459, 640
VpaK11BI GGWCC 1 cut(s) 577
XbaI TCTAGA 1 cut(s) 125
XceI RCATGY 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XhoI CTCGAG 1 cut(s) 96
XspI CTAG 3 cut(s) 126, 173, 216
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.