Rw5G017170

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000201
Physical Location & Seq
Reverse (-)
21922387 .. 21923646
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017170.1

Sequence Viewer

Length: 840 bp
ATGAGGGAGTTCAAAAATGAAGTTCTACTGATAGCCAAACTTCAGCACCGTAATCTTGTAAAGCTTCTGGGCTGTTGCATTCAAAATGATGAAAAAATGTTAATATATGAATACATGTCCAACAGGAGCTTGGACTTCTTTATATTTGATCAGGAAAGACAGAAATCGCTCGACTGGCCTTCCTGCTACAACATTATTGACGGAACTGCACGAGGGCTTCTCTATCTTCACCAAGACTCGAGATTAAGAATTATTCACAGAGATCTGAAACCTAGTAATATTTTGCTAGATGAAAACATGAACCCAAGGATTTCAGATTTTGGCCTGGCTAAAACATTTAGATTTGATCAAAGTCAAGCCAATACGAATAAAGTGGCTGGAACATATGGTTATATGGCACCTGAATATGCAGTAGATGGAATTTTCTCGACGAAATCAGATGTATTTAGCTTTGGAGTTGTGCTGATTGAGTTATTGAGTAGGGAGAAGAACAGGGGATTTTGTCATCCAGATCACGATTTTAATCTTCTTGGACATGCATGGACACTATGGACACAAAATGTACCACTGGAACTCATCGATAAGACATTATGTGATCCGGATACCATATCCGAAGTTTTAAGGTGTCTTCATGTGGGTCTGTTATGTGTGCAGCAAGTACCTGAAGATAGACCAAGCATGTCATCTGTGGTACTAATGTTGGGCAGTCATGATGTTGTGCTGCCTTTGCCAAAGCAGCCTGGTTTTTACACTGAACGGACTCTTCCCGAATCATCATCAAGGTCACGTGACCTGTGTTCACCCAGTAACTTCAGCACTACATTGTTACAGGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

279

Amino Acids

31.99

Weight (kDa)

6.07

Isoelectric Point (pI)

53.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 2 - 231 1.6e-38 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 4 - 230 1.5e-38 Protein kinase domain
DUF3403 PF11883 237 - 279 9.8e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 397
AccIII TCCGGA 1 cut(s) 598
AclWI GGATC 1 cut(s) 590
AcsI RAATTY 1 cut(s) 420
AcuI CTGAAG 3 cut(s) 26, 684, 796
AcvI CACGTG 1 cut(s) 788
AfaI GTAC 3 cut(s) 564, 660, 693
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 2 cut(s) 13, 83
AjnI CCWGG 2 cut(s) 324, 739
AluBI AGCT 3 cut(s) 64, 129, 450
AluI AGCT 3 cut(s) 64, 129, 450
AlwI GGATC 1 cut(s) 590
Ama87I CYCGRG 1 cut(s) 238
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 2 cut(s) 176, 322
ApeKI GCWGC 3 cut(s) 652, 721, 736
ApoI RAATTY 1 cut(s) 420
AsuHPI GGTGA 2 cut(s) 221, 792
AvaI CYCGRG 1 cut(s) 238
BaeI ACNNNNGTAYC 2 cut(s) 683, 716
BanI GGYRCC 1 cut(s) 397
BauI CACGAG 1 cut(s) 210
BbrPI CACGTG 1 cut(s) 788
BbsI GAAGAC 1 cut(s) 620
BbvI GCAGC 3 cut(s) 664, 708, 748
BccI CCATC 1 cut(s) 410
BciT130I CCWGG 2 cut(s) 326, 741
BciVI GTATCC 1 cut(s) 595
BclI TGATCA 2 cut(s) 148, 346
BfaI CTAG 2 cut(s) 273, 287
BfuI GTATCC 1 cut(s) 595
BglII AGATCT 1 cut(s) 262
BisI GCNGC 3 cut(s) 653, 722, 737
BlsI GCNGC 3 cut(s) 654, 723, 738
Bme1390I CCNGG 2 cut(s) 326, 741
BmeT110I CYCGRG 1 cut(s) 238
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 2 cut(s) 326, 741
BmrI ACTGGG 1 cut(s) 798
BmuI ACTGGG 1 cut(s) 798
BpiI GAAGAC 1 cut(s) 620
BplI GAGNNNNNCTC 2 cut(s) 204, 236
Bsa29I ATCGAT 1 cut(s) 579
BsaAI YACGTR 1 cut(s) 788
BsaBI GATNNNNATC 1 cut(s) 522
BsaJI CCNNGG 1 cut(s) 305
BsaWI WCCGGW 1 cut(s) 598
Bse1I ACTGG 3 cut(s) 179, 573, 804
Bse8I GATNNNNATC 1 cut(s) 522
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 2 cut(s) 326, 741
BseCI ATCGAT 1 cut(s) 579
BseDI CCNNGG 1 cut(s) 305
BseGI GGATG 1 cut(s) 505
BseJI GATNNNNATC 1 cut(s) 522
BseNI ACTGG 3 cut(s) 179, 573, 804
BseXI GCAGC 3 cut(s) 664, 708, 748
BsgI GTGCAG 2 cut(s) 192, 671
BshFI GGCC 2 cut(s) 178, 324
BshNI GGYRCC 1 cut(s) 397
BshVI ATCGAT 1 cut(s) 579
BsiHKCI CYCGRG 1 cut(s) 238
BsiSI CCGG 1 cut(s) 599
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 2 cut(s) 178, 324
BsoBI CYCGRG 1 cut(s) 238
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 5 cut(s) 148, 262, 346, 511, 595
BspANI GGCC 2 cut(s) 178, 324
BspDI ATCGAT 1 cut(s) 579
BspEI TCCGGA 1 cut(s) 598
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 1 cut(s) 399
BspPI GGATC 1 cut(s) 590
BspT107I GGYRCC 1 cut(s) 397
BsrI ACTGG 3 cut(s) 179, 573, 804
BssECI CCNNGG 1 cut(s) 305
BssMI GATC 5 cut(s) 148, 262, 346, 511, 595
BssSI CACGAG 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 305
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 2 cut(s) 326, 741
Bst4CI ACNGT 1 cut(s) 50
Bst6I CTCTTC 1 cut(s) 768
BstBAI YACGTR 1 cut(s) 788
BstF5I GGATG 1 cut(s) 505
BstKTI GATC 5 cut(s) 151, 265, 349, 514, 598
BstMBI GATC 5 cut(s) 148, 262, 346, 511, 595
BstMWI GCNNNNNNNGC 3 cut(s) 175, 727, 736
BstNI CCWGG 2 cut(s) 326, 741
BstNSI RCATGY 3 cut(s) 118, 539, 682
BstSCI CCNGG 2 cut(s) 324, 739
BstV1I GCAGC 3 cut(s) 664, 708, 748
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
Bsu15I ATCGAT 1 cut(s) 579
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 2 cut(s) 178, 324
BsuTUI ATCGAT 1 cut(s) 579
BtsCI GGATG 1 cut(s) 505
BtsIMutI CAGTG 2 cut(s) 566, 750
CciI TCATGA 1 cut(s) 709
ClaI ATCGAT 1 cut(s) 579
Csp6I GTAC 3 cut(s) 563, 659, 692
CviAII CATG 7 cut(s) 115, 298, 536, 540, 632, 679, 710
CviQI GTAC 3 cut(s) 563, 659, 692
DpnI GATC 5 cut(s) 150, 264, 348, 513, 597
DpnII GATC 5 cut(s) 148, 262, 346, 511, 595
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco130I CCWWGG 1 cut(s) 305
Eco57I CTGAAG 3 cut(s) 26, 684, 796
Eco72I CACGTG 1 cut(s) 788
Eco88I CYCGRG 1 cut(s) 238
EcoRII CCWGG 2 cut(s) 324, 739
EcoT14I CCWWGG 1 cut(s) 305
EcoT22I ATGCAT 1 cut(s) 541
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 7 cut(s) 118, 301, 539, 543, 635, 682, 713
FatI CATG 7 cut(s) 114, 297, 535, 539, 631, 678, 709
FauNDI CATATG 1 cut(s) 385
FbaI TGATCA 2 cut(s) 148, 346
Fnu4HI GCNGC 3 cut(s) 653, 722, 737
FokI GGATG 1 cut(s) 492
Fsp4HI GCNGC 3 cut(s) 653, 722, 737
FspBI CTAG 2 cut(s) 273, 287
GluI GCNGC 3 cut(s) 653, 722, 737
HaeIII GGCC 2 cut(s) 178, 324
HapII CCGG 1 cut(s) 599
Hin1II CATG 7 cut(s) 118, 301, 539, 543, 635, 682, 713
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 3 cut(s) 236, 760, 770
HpaII CCGG 1 cut(s) 599
HphI GGTGA 2 cut(s) 221, 792
Hpy166II GTNNAC 1 cut(s) 800
Hpy188I TCNGA 4 cut(s) 267, 316, 439, 613
Hpy188III TCNNGA 8 cut(s) 152, 240, 427, 509, 515, 599, 710, 767
Hpy8I GTNNAC 1 cut(s) 800
Hpy99I CGWCG 1 cut(s) 433
HpyAV CCTTC 1 cut(s) 189
HpyCH4III ACNGT 1 cut(s) 50
HpyCH4IV ACGT 1 cut(s) 787
HpyCH4V TGCA 5 cut(s) 78, 209, 410, 539, 652
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 727, 736
HpySE526I ACGT 1 cut(s) 787
Hsp92II CATG 7 cut(s) 118, 301, 539, 543, 635, 682, 713
Kpn2I TCCGGA 1 cut(s) 598
Ksp22I TGATCA 2 cut(s) 148, 346
Kzo9I GATC 5 cut(s) 148, 262, 346, 511, 595
LmnI GCTCC 1 cut(s) 126
Lsp1109I GCAGC 3 cut(s) 664, 708, 748
MaeI CTAG 2 cut(s) 273, 287
MaeII ACGT 1 cut(s) 787
MaeIII GTNAC 4 cut(s) 783, 788, 806, 825
MalI GATC 5 cut(s) 150, 264, 348, 513, 597
MboI GATC 5 cut(s) 148, 262, 346, 511, 595
MboII GAAGA 6 cut(s) 218, 499, 518, 620, 677, 755
MflI RGATCY 1 cut(s) 262
MluCI AATT 2 cut(s) 249, 420
MlyI GAGTC 2 cut(s) 230, 754
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 1 cut(s) 206
Mph1103I ATGCAT 1 cut(s) 541
MroI TCCGGA 1 cut(s) 598
MseI TTAA 4 cut(s) 101, 245, 522, 620
MspI CCGG 1 cut(s) 599
MspR9I CCNGG 2 cut(s) 326, 741
Mva1269I GAATGC 1 cut(s) 78
MvaI CCWGG 2 cut(s) 326, 741
MwoI GCNNNNNNNGC 3 cut(s) 175, 727, 736
NdeI CATATG 1 cut(s) 385
NdeII GATC 5 cut(s) 148, 262, 346, 511, 595
NlaIII CATG 7 cut(s) 118, 301, 539, 543, 635, 682, 713
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 2 cut(s) 783, 788
NsiI ATGCAT 1 cut(s) 541
NspI RCATGY 3 cut(s) 118, 539, 682
PaeR7I CTCGAG 1 cut(s) 238
PagI TCATGA 1 cut(s) 709
PciI ACATGT 1 cut(s) 114
PctI GAATGC 1 cut(s) 78
PfeI GAWTC 1 cut(s) 770
PkrI GCNGC 3 cut(s) 654, 723, 738
PleI GAGTC 2 cut(s) 230, 754
PmaCI CACGTG 1 cut(s) 788
PmlI CACGTG 1 cut(s) 788
PpsI GAGTC 2 cut(s) 230, 754
Ppu21I YACGTR 1 cut(s) 788
PscI ACATGT 1 cut(s) 114
Psp6I CCWGG 2 cut(s) 324, 739
PspCI CACGTG 1 cut(s) 788
PspGI CCWGG 2 cut(s) 324, 739
PspN4I GGNNCC 1 cut(s) 399
PsuI RGATCY 1 cut(s) 262
RsaI GTAC 3 cut(s) 564, 660, 693
RsaNI GTAC 3 cut(s) 563, 659, 692
SaqAI TTAA 4 cut(s) 101, 245, 522, 620
SatI GCNGC 3 cut(s) 653, 722, 737
Sau3AI GATC 5 cut(s) 148, 262, 346, 511, 595
SchI GAGTC 2 cut(s) 230, 754
ScrFI CCNGG 2 cut(s) 326, 741
Sfr274I CTCGAG 1 cut(s) 238
SlaI CTCGAG 1 cut(s) 238
SmlI CTYRAG 1 cut(s) 238
SmoI CTYRAG 1 cut(s) 238
Sse9I AATT 2 cut(s) 249, 420
SspI AATATT 1 cut(s) 280
SspMI CTAG 2 cut(s) 273, 287
StyD4I CCNGG 2 cut(s) 324, 739
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 1 cut(s) 50
TaiI ACGT 1 cut(s) 790
TaqI TCGA 4 cut(s) 171, 239, 428, 579
TasI AATT 2 cut(s) 249, 420
TfiI GAWTC 1 cut(s) 770
Tru1I TTAA 4 cut(s) 101, 245, 522, 620
Tru9I TTAA 4 cut(s) 101, 245, 522, 620
TscAI CASTG 2 cut(s) 573, 757
TseFI GTSAC 2 cut(s) 783, 788
TseI GCWGC 3 cut(s) 652, 721, 736
Tsp45I GTSAC 2 cut(s) 783, 788
TspDTI ATGAA 6 cut(s) 33, 105, 123, 306, 314, 620
TspGWI ACGGA 2 cut(s) 216, 772
TspRI CASTG 2 cut(s) 573, 757
XapI RAATTY 1 cut(s) 420
XceI RCATGY 3 cut(s) 118, 539, 682
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XhoI CTCGAG 1 cut(s) 238
XspI CTAG 2 cut(s) 273, 287
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.