Rh5DG258000

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000201
Physical Location & Seq
Forward (+)
30545273 .. 30577007
31735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG258000.1

Sequence Viewer

Length: 588 bp
ATGGCTGAGGAGGCTGAAAAGCTTAAAGATGAAGTAGAGGCACTGCAACAAGAAAAGTCAAGTTTCACTTGTGCTTTTCGGCATATAGAGTTCAGGTTTGACGAACTCAGAGACCTCTGGCAGGGAATACTTGTCTCGGCCTCTTCTAATGGAGTATGGGTGGTTGCATACGTTTTGAATGAAATTCATGATGATATCTCATGGCTTCAAGTTGCCTCCAGTTTTGTGCTCTTAGTAACGACGATCATTCTTGTATTAGCAAACATGCTGGACATGAAGGTGTGGACTCTATGGACTGAAGGAAGATCATTGGAAGTAATGGATAAGAAGTTAGATGGCTCCTATGCTCTAACAGTAGTGTCACGATGCATTCACATAGCTCTGTTGTGTGTGCAACAACAACCAGAAGATAGGCCAAACATGGCATCTGTGGTCATAATGTTGGGAAGTGAGGGTTCATTGCCTGCGCCAAAGCAACCTGGTTTCTTCACTGAAAGGTATCCGTTTGAAGTAGAGAACTCTTCATCAAACAATTTGGAATCACATACAATAAATGGCATGAGTATAACACAGTTAGAGGCACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

195

Amino Acids

22.01

Weight (kDa)

4.7

Isoelectric Point (pI)

54.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3403 PF11883 151 - 195 1.4e-11 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 318
AfiI CCNNNNNNNGG 1 cut(s) 121
AgsI TTSAA 3 cut(s) 178, 209, 509
AjnI CCWGG 1 cut(s) 478
AluBI AGCT 2 cut(s) 22, 380
AluI AGCT 2 cut(s) 22, 380
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 2 cut(s) 105, 139
AoxI GGCC 2 cut(s) 138, 413
ApoI RAATTY 1 cut(s) 183
AspLEI GCGC 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 231
BbvCI CCTCAGC 1 cut(s) 6
BccI CCATC 1 cut(s) 329
BciT130I CCWGG 1 cut(s) 480
BciVI GTATCC 1 cut(s) 510
BcoDI GTCTC 2 cut(s) 105, 139
BfuI GTATCC 1 cut(s) 510
Bme1390I CCNGG 1 cut(s) 480
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 1 cut(s) 480
BmsI GCATC 2 cut(s) 356, 434
BpmI CTGGAG 1 cut(s) 202
Bpu10I CCTNAGC 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 219
Bse3DI GCAATG 1 cut(s) 458
BseBI CCWGG 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMI GCAATG 1 cut(s) 458
BseMII CTCAG 1 cut(s) 121
BseNI ACTGG 1 cut(s) 219
BseRI GAGGAG 1 cut(s) 23
BshFI GGCC 2 cut(s) 140, 415
BsiHKAI GWGCWC 1 cut(s) 231
BslI CCNNNNNNNGG 1 cut(s) 121
BsmAI GTCTC 2 cut(s) 105, 139
BsmI GAATGC 1 cut(s) 369
BsnI GGCC 2 cut(s) 140, 415
Bso31I GGTCTC 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 2 cut(s) 243, 305
BspANI GGCC 2 cut(s) 140, 415
BspCNI CTCAG 1 cut(s) 120
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 458
BsrI ACTGG 1 cut(s) 219
BssMI GATC 2 cut(s) 243, 305
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 2 cut(s) 355, 573
Bst6I CTCTTC 2 cut(s) 148, 526
BstC8I GCNNGC 1 cut(s) 465
BstDEI CTNAG 3 cut(s) 6, 107, 232
BstENI CCTNNNNNAGG 1 cut(s) 119
BstHHI GCGC 1 cut(s) 469
BstKTI GATC 2 cut(s) 246, 308
BstMAI GTCTC 2 cut(s) 105, 139
BstMBI GATC 2 cut(s) 243, 305
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 478
BsuI GTATCC 1 cut(s) 510
BsuRI GGCC 2 cut(s) 140, 415
BtsI GCAGTG 1 cut(s) 41
BtsIMutI CAGTG 2 cut(s) 41, 489
Cac8I GCNNGC 1 cut(s) 465
CciI TCATGA 1 cut(s) 187
CfoI GCGC 1 cut(s) 469
CsiI ACCWGGT 1 cut(s) 478
CviAII CATG 6 cut(s) 188, 201, 265, 274, 421, 559
CviJI RGCY 8 cut(s) 5, 14, 22, 140, 205, 339, 380, 415
CviKI_1 RGCY 8 cut(s) 5, 14, 22, 140, 205, 339, 380, 415
DdeI CTNAG 3 cut(s) 6, 107, 232
DpnI GATC 2 cut(s) 245, 307
DpnII GATC 2 cut(s) 243, 305
Eam1104I CTCTTC 2 cut(s) 148, 526
EarI CTCTTC 2 cut(s) 148, 526
Eco31I GGTCTC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 478
EcoRV GATATC 1 cut(s) 196
EcoT22I ATGCAT 1 cut(s) 371
FaeI CATG 6 cut(s) 191, 204, 268, 277, 424, 562
FalI AAGNNNNNCTT 2 cut(s) 52, 84
FatI CATG 6 cut(s) 187, 200, 264, 273, 420, 558
GlaI GCGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 202
HaeIII GGCC 2 cut(s) 140, 415
HhaI GCGC 1 cut(s) 469
Hin1II CATG 6 cut(s) 191, 204, 268, 277, 424, 562
Hin6I GCGC 1 cut(s) 467
HinP1I GCGC 1 cut(s) 467
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 2 cut(s) 286, 539
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 1 cut(s) 110
Hpy188III TCNNGA 2 cut(s) 188, 363
Hpy8I GTNNAC 1 cut(s) 285
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 2 cut(s) 271, 293
HpyCH4III ACNGT 2 cut(s) 355, 573
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 4 cut(s) 46, 167, 369, 394
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 3 cut(s) 6, 107, 232
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 6 cut(s) 191, 204, 268, 277, 424, 562
HspAI GCGC 1 cut(s) 467
Kzo9I GATC 2 cut(s) 243, 305
LmnI GCTCC 1 cut(s) 344
LpnPI CCDG 9 cut(s) 79, 103, 107, 232, 254, 417, 465, 477, 492
LweI GCATC 2 cut(s) 356, 434
MabI ACCWGGT 1 cut(s) 478
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 235, 360
MalI GATC 2 cut(s) 245, 307
MboI GATC 2 cut(s) 243, 305
MboII GAAGA 5 cut(s) 135, 315, 419, 478, 513
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 2 cut(s) 183, 532
MlyI GAGTC 1 cut(s) 280
MnlI CCTC 7 cut(s) 4, 31, 125, 151, 226, 445, 571
Mph1103I ATGCAT 1 cut(s) 371
MseI TTAA 1 cut(s) 24
MslI CAYNNNNRTG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 480
Mva1269I GAATGC 1 cut(s) 369
MvaI CCWGG 1 cut(s) 480
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 2 cut(s) 243, 305
NlaIII CATG 6 cut(s) 191, 204, 268, 277, 424, 562
NlaIV GGNNCC 1 cut(s) 340
NmeAIII GCCGAG 1 cut(s) 116
NmuCI GTSAC 1 cut(s) 360
NsiI ATGCAT 1 cut(s) 371
NspI RCATGY 1 cut(s) 268
PagI TCATGA 1 cut(s) 187
PctI GAATGC 1 cut(s) 369
PfeI GAWTC 1 cut(s) 539
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 478
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 340
RseI CAYNNNNRTG 1 cut(s) 278
SaqAI TTAA 1 cut(s) 24
Sau3AI GATC 2 cut(s) 243, 305
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 480
SduI GDGCHC 1 cut(s) 231
SetI ASST 8 cut(s) 24, 98, 117, 174, 282, 382, 481, 500
SexAI ACCWGGT 1 cut(s) 478
SfaNI GCATC 2 cut(s) 356, 434
SmiMI CAYNNNNRTG 1 cut(s) 278
Sse9I AATT 2 cut(s) 183, 532
StyD4I CCNGG 1 cut(s) 478
TaaI ACNGT 2 cut(s) 355, 573
TaiI ACGT 1 cut(s) 174
TasI AATT 2 cut(s) 183, 532
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TscAI CASTG 2 cut(s) 48, 496
TseFI GTSAC 1 cut(s) 360
Tsp45I GTSAC 1 cut(s) 360
TspDTI ATGAA 6 cut(s) 45, 176, 195, 290, 447, 513
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 2 cut(s) 48, 496
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 1 cut(s) 183
XceI RCATGY 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.