RLG00000032899

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000201
Physical Location & Seq
Reverse (-)
20596302 .. 20599012
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032899

Sequence Viewer

Length: 405 bp
ATGCCAGCTGTAAAGTCTGACTCTGGTTCTGCTGCAACTGTGACAAGTGGTTACATGTCCCCAGAATACACAATTGATGGTTTCTACTCAATAAAATCCGATGTCTACAACTATGGTGCTAGAGAGAGTGAGTTGGAAGAGCAACTAAGGATTCTCGCATCCAGGCCACAATCTCAACCTTTTTGGACATGTGAGGTCTTTTATGCATGTGTACAACAAAAACCACAAGATAGGCCAAGTATGCCAGCTGCTGTTCTAATATTGAGTGGTGAAGGTGCATTGCCTCAACCTCAGAAGCCTGGTTTTTTTAGTGAAAGGAATCTGACAGAACTTGAAGATTCTACAGATGAGGCTACAGAATCTTCAGCTAATGAACTCACTATTACACAAGTGGGGGCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

135

Amino Acids

14.7

Weight (kDa)

4.39

Isoelectric Point (pI)

77.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3403 PF11883 91 - 134 1.2e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 105
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 1 cut(s) 213
AflIII ACRYGT 2 cut(s) 54, 188
AgsI TTSAA 1 cut(s) 335
AjnI CCWGG 2 cut(s) 161, 298
AluBI AGCT 3 cut(s) 8, 248, 368
AluI AGCT 3 cut(s) 8, 248, 368
AlwNI CAGNNNCTG 1 cut(s) 251
AoxI GGCC 2 cut(s) 164, 233
ApeKI GCWGC 2 cut(s) 32, 248
AsuHPI GGTGA 1 cut(s) 281
BanII GRGCYC 1 cut(s) 400
BbvI GCAGC 2 cut(s) 19, 235
BccI CCATC 1 cut(s) 71
BciT130I CCWGG 2 cut(s) 163, 300
BfaI CTAG 1 cut(s) 120
BfmI CTRYAG 2 cut(s) 342, 354
BisI GCNGC 2 cut(s) 33, 249
BlsI GCNGC 2 cut(s) 34, 250
Bme1390I CCNGG 2 cut(s) 163, 300
BmrFI CCNGG 2 cut(s) 163, 300
BmsI GCATC 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 278
BseBI CCWGG 2 cut(s) 163, 300
BseGI GGATG 1 cut(s) 158
BseMI GCAATG 1 cut(s) 278
BseMII CTCAG 1 cut(s) 305
BseXI GCAGC 2 cut(s) 19, 235
BshFI GGCC 2 cut(s) 166, 235
BslFI GGGAC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 43
BsnI GGCC 2 cut(s) 166, 235
Bsp1286I GDGCHC 1 cut(s) 400
Bsp1407I TGTACA 1 cut(s) 211
BspANI GGCC 2 cut(s) 166, 235
BspCNI CTCAG 1 cut(s) 304
BspQI GCTCTTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 278
BsrGI TGTACA 1 cut(s) 211
Bst2UI CCWGG 2 cut(s) 163, 300
Bst4CI ACNGT 1 cut(s) 40
Bst6I CTCTTC 1 cut(s) 132
BstAUI TGTACA 1 cut(s) 211
BstC8I GCNNGC 2 cut(s) 6, 246
BstDEI CTNAG 2 cut(s) 146, 291
BstF5I GGATG 1 cut(s) 158
BstMWI GCNNNNNNNGC 1 cut(s) 241
BstNI CCWGG 2 cut(s) 163, 300
BstNSI RCATGY 3 cut(s) 58, 192, 210
BstSCI CCNGG 2 cut(s) 161, 298
BstSFI CTRYAG 2 cut(s) 342, 354
BstV1I GCAGC 2 cut(s) 19, 235
BsuRI GGCC 2 cut(s) 166, 235
BtsCI GGATG 1 cut(s) 158
Cac8I GCNNGC 2 cut(s) 6, 246
CaiI CAGNNNCTG 1 cut(s) 251
Csp6I GTAC 1 cut(s) 212
CviAII CATG 3 cut(s) 55, 189, 207
CviJI RGCY 8 cut(s) 8, 166, 235, 248, 298, 353, 368, 398
CviKI_1 RGCY 8 cut(s) 8, 166, 235, 248, 298, 353, 368, 398
CviQI GTAC 1 cut(s) 212
DdeI CTNAG 2 cut(s) 146, 291
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 348
EcoRII CCWGG 2 cut(s) 161, 298
EcoT22I ATGCAT 1 cut(s) 208
EcoT38I GRGCYC 1 cut(s) 400
FaeI CATG 3 cut(s) 58, 192, 210
FaiI YATR 6 cut(s) 56, 114, 190, 204, 208, 242
FaqI GGGAC 1 cut(s) 43
FatI CATG 3 cut(s) 54, 188, 206
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 2 cut(s) 33, 249
FokI GGATG 1 cut(s) 145
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 2 cut(s) 33, 249
FspBI CTAG 1 cut(s) 120
GluI GCNGC 2 cut(s) 33, 249
HaeIII GGCC 2 cut(s) 166, 235
Hin1II CATG 3 cut(s) 58, 192, 210
HinfI GANTC 5 cut(s) 20, 151, 319, 338, 359
HphI GGTGA 1 cut(s) 281
Hpy166II GTNNAC 2 cut(s) 106, 212
Hpy188I TCNGA 4 cut(s) 19, 100, 294, 324
Hpy8I GTNNAC 2 cut(s) 106, 212
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4V TGCA 3 cut(s) 35, 206, 278
HpyF10VI GCNNNNNNNGC 1 cut(s) 241
HpyF3I CTNAG 2 cut(s) 146, 291
Hsp92II CATG 3 cut(s) 58, 192, 210
LguI GCTCTTC 1 cut(s) 132
LpnPI CCDG 8 cut(s) 9, 18, 75, 148, 175, 258, 285, 312
Lsp1109I GCAGC 2 cut(s) 19, 235
LweI GCATC 1 cut(s) 167
MaeI CTAG 1 cut(s) 120
MaeIII GTNAC 2 cut(s) 40, 50
MboII GAAGA 3 cut(s) 149, 347, 354
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 1 cut(s) 72
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 4 cut(s) 187, 294, 300, 343
Mph1103I ATGCAT 1 cut(s) 208
MspA1I CMGCKG 2 cut(s) 8, 248
MspR9I CCNGG 2 cut(s) 163, 300
MunI CAATTG 1 cut(s) 72
MvaI CCWGG 2 cut(s) 163, 300
MwoI GCNNNNNNNGC 1 cut(s) 241
NlaIII CATG 3 cut(s) 58, 192, 210
NmuCI GTSAC 1 cut(s) 40
NsiI ATGCAT 1 cut(s) 208
NspI RCATGY 3 cut(s) 58, 192, 210
PciI ACATGT 2 cut(s) 54, 188
PciSI GCTCTTC 1 cut(s) 132
PfeI GAWTC 4 cut(s) 151, 319, 338, 359
PkrI GCNGC 2 cut(s) 34, 250
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
PscI ACATGT 2 cut(s) 54, 188
Psp6I CCWGG 2 cut(s) 161, 298
PspGI CCWGG 2 cut(s) 161, 298
PstNI CAGNNNCTG 1 cut(s) 251
PvuII CAGCTG 2 cut(s) 8, 248
RsaI GTAC 1 cut(s) 213
RsaNI GTAC 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 132
SatI GCNGC 2 cut(s) 33, 249
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 163, 300
SduI GDGCHC 1 cut(s) 400
SetI ASST 7 cut(s) 10, 181, 198, 250, 277, 292, 370
SfaNI GCATC 1 cut(s) 167
SfcI CTRYAG 2 cut(s) 342, 354
Sse9I AATT 1 cut(s) 72
SspI AATATT 1 cut(s) 261
SspMI CTAG 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 161, 298
TaaI ACNGT 1 cut(s) 40
TaqI TCGA 1 cut(s) 400
TasI AATT 1 cut(s) 72
TatI WGTACW 1 cut(s) 211
TfiI GAWTC 4 cut(s) 151, 319, 338, 359
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 2 cut(s) 32, 248
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 1 cut(s) 387
XceI RCATGY 3 cut(s) 58, 192, 210
XmiI GTMKAC 1 cut(s) 105
XspI CTAG 1 cut(s) 120
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.