MD12G1047500.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000201
Physical Location & Seq
Forward (+)
5436649 .. 5437861
1213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1047500.v1.1.491

Sequence Viewer

Length: 636 bp
ATGAGGGTGTTCATGAATGAAGGACAAAAATTGCTCGACTGGACTAAGTGCTTCCACATTATTGGTGGAATTGCTCGAGGACTTCTTTACCTTCACCATGACTTTAGAGTAAGGATTATCCATCGAGATCTAAAAGCTAGCAATATTCTCCTTGATAATAATATGAACCCAAAGATTGCAGACTTCGGCTTGGCTAAAATATTCGACAGTGATCAAAGTCAGGCCAATATAAATAGAGCGGTTGGAACCTACTTTGGAGTTATACTGTTAGAGATGTTGAGTAAGAAGAAGAACAGGGGATTTAGTCATCCTGATCACCACCTTAACCTTCTTGGACACGCATGGACACTATGGATGCAAGATAAACAACTGGAACTAATCGATAAGATGCTTTCTGATTCGTGCAACATATTTGAAGTTGTAAGGTGCCTTCATGTGGGGCTGTTATGCGTGCAAAGAGTACCTGAGGATAGACCAAGCATGTCATCTGTGGTTCTGATGTTGAGCAGTTATGTGGCCTTGCCGCCTCCAAAGCAGCCTGATTTCTACATTGAACGAAACGTCTCCGAATCACCATCAAGGACGCTTCCATGTTTAGAGAATACTTTCAGCACTACGTTGATAGAACCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

212

Amino Acids

24.21

Weight (kDa)

8.29

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 3 - 74 1.6e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 11 - 83 3.4e-15 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 426
AccBSI CCGCTC 1 cut(s) 239
AciI CCGC 2 cut(s) 239, 524
AfaI GTAC 1 cut(s) 462
AfiI CCNNNNNNNGG 1 cut(s) 436
AgsI TTSAA 2 cut(s) 416, 554
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 568
Ama87I CYCGRG 1 cut(s) 75
AoxI GGCC 2 cut(s) 222, 516
ApeKI GCWGC 1 cut(s) 535
Asp700I GAANNNNTTC 1 cut(s) 605
AsuHPI GGTGA 3 cut(s) 86, 308, 564
AsuNHI GCTAGC 1 cut(s) 137
AvaI CYCGRG 1 cut(s) 75
AxyI CCTNAGG 1 cut(s) 465
BanI GGYRCC 1 cut(s) 426
BarI GAAGNNNNNNTAC 2 cut(s) 414, 446
BbvI GCAGC 1 cut(s) 547
BccI CCATC 2 cut(s) 129, 583
BclI TGATCA 2 cut(s) 211, 313
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 1 cut(s) 138
BglII AGATCT 1 cut(s) 127
BisI GCNGC 2 cut(s) 524, 536
BlsI GCNGC 2 cut(s) 525, 537
BmeT110I CYCGRG 1 cut(s) 75
BmiI GGNNCC 2 cut(s) 247, 428
BmsI GCATC 2 cut(s) 345, 378
BmtI GCTAGC 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 629
Bsc4I CCNNNNNNNGG 1 cut(s) 436
Bse1I ACTGG 2 cut(s) 44, 375
Bse21I CCTNAGG 1 cut(s) 465
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 629
BseGI GGATG 2 cut(s) 307, 360
BseLI CCNNNNNNNGG 1 cut(s) 436
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 2 cut(s) 44, 375
BseXI GCAGC 1 cut(s) 547
BshFI GGCC 2 cut(s) 224, 518
BshNI GGYRCC 1 cut(s) 426
BshVI ATCGAT 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 75
BslI CCNNNNNNNGG 1 cut(s) 436
BsmAI GTCTC 1 cut(s) 568
BsmBI CGTCTC 1 cut(s) 568
BsnI GGCC 2 cut(s) 224, 518
BsoBI CYCGRG 1 cut(s) 75
Bsp143I GATC 3 cut(s) 127, 211, 313
BspACI CCGC 2 cut(s) 239, 524
BspANI GGCC 2 cut(s) 224, 518
BspCNI CTCAG 1 cut(s) 457
BspDI ATCGAT 1 cut(s) 381
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 2 cut(s) 247, 428
BspOI GCTAGC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 426
BsrBI CCGCTC 1 cut(s) 239
BsrI ACTGG 2 cut(s) 44, 375
BssECI CCNNGG 1 cut(s) 629
BssMI GATC 3 cut(s) 127, 211, 313
Bst4CI ACNGT 2 cut(s) 209, 267
BstC8I GCNNGC 2 cut(s) 139, 452
BstDEI CTNAG 2 cut(s) 45, 465
BstF5I GGATG 2 cut(s) 307, 360
BstKTI GATC 3 cut(s) 130, 214, 316
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 3 cut(s) 127, 211, 313
BstMWI GCNNNNNNNGC 1 cut(s) 532
BstNSI RCATGY 1 cut(s) 484
BstV1I GCAGC 1 cut(s) 547
BstX2I RGATCY 1 cut(s) 127
BstXI CCANNNNNNTGG 1 cut(s) 62
BstYI RGATCY 1 cut(s) 127
Bsu15I ATCGAT 1 cut(s) 381
Bsu36I CCTNAGG 1 cut(s) 465
BsuRI GGCC 2 cut(s) 224, 518
BsuTUI ATCGAT 1 cut(s) 381
BtsCI GGATG 2 cut(s) 307, 360
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 139, 452
CciI TCATGA 1 cut(s) 12
ClaI ATCGAT 1 cut(s) 381
CseI GACGC 1 cut(s) 592
Csp6I GTAC 1 cut(s) 461
CviAII CATG 6 cut(s) 13, 98, 342, 434, 481, 591
CviJI RGCY 7 cut(s) 137, 189, 194, 224, 442, 518, 538
CviKI_1 RGCY 7 cut(s) 137, 189, 194, 224, 442, 518, 538
CviQI GTAC 1 cut(s) 461
DdeI CTNAG 2 cut(s) 45, 465
DpnI GATC 3 cut(s) 129, 213, 315
DpnII GATC 3 cut(s) 127, 211, 313
Eco81I CCTNAGG 1 cut(s) 465
Eco88I CYCGRG 1 cut(s) 75
Esp3I CGTCTC 1 cut(s) 568
FaeI CATG 6 cut(s) 16, 101, 345, 437, 484, 594
FatI CATG 6 cut(s) 12, 97, 341, 433, 480, 590
FbaI TGATCA 2 cut(s) 211, 313
Fnu4HI GCNGC 2 cut(s) 524, 536
FokI GGATG 2 cut(s) 294, 367
Fsp4HI GCNGC 2 cut(s) 524, 536
FspBI CTAG 1 cut(s) 138
GluI GCNGC 2 cut(s) 524, 536
HaeIII GGCC 2 cut(s) 224, 518
HgaI GACGC 1 cut(s) 592
Hin1II CATG 6 cut(s) 16, 101, 345, 437, 484, 594
HinfI GANTC 2 cut(s) 398, 569
HphI GGTGA 3 cut(s) 86, 308, 564
Hpy188I TCNGA 3 cut(s) 397, 498, 568
Hpy188III TCNNGA 3 cut(s) 13, 125, 311
HpyAV CCTTC 4 cut(s) 14, 101, 338, 440
HpyCH4III ACNGT 2 cut(s) 209, 267
HpyCH4IV ACGT 2 cut(s) 561, 617
HpyCH4V TGCA 4 cut(s) 179, 358, 405, 454
HpyF10VI GCNNNNNNNGC 1 cut(s) 532
HpyF3I CTNAG 2 cut(s) 45, 465
HpySE526I ACGT 2 cut(s) 561, 617
Hsp92II CATG 6 cut(s) 16, 101, 345, 437, 484, 594
Ksp22I TGATCA 2 cut(s) 211, 313
Kzo9I GATC 3 cut(s) 127, 211, 313
LpnPI CCDG 7 cut(s) 25, 206, 280, 324, 356, 477, 552
Lsp1109I GCAGC 1 cut(s) 547
LweI GCATC 2 cut(s) 345, 378
MaeI CTAG 1 cut(s) 138
MaeII ACGT 2 cut(s) 561, 617
MalI GATC 3 cut(s) 129, 213, 315
MbiI CCGCTC 1 cut(s) 239
MboI GATC 3 cut(s) 127, 211, 313
MboII GAAGA 2 cut(s) 298, 301
MflI RGATCY 1 cut(s) 127
MluCI AATT 2 cut(s) 29, 69
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 3 cut(s) 71, 460, 537
MroXI GAANNNNTTC 1 cut(s) 605
MseI TTAA 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 532
NdeII GATC 3 cut(s) 127, 211, 313
NheI GCTAGC 1 cut(s) 137
NlaIII CATG 6 cut(s) 16, 101, 345, 437, 484, 594
NlaIV GGNNCC 2 cut(s) 247, 428
NspI RCATGY 1 cut(s) 484
PaeR7I CTCGAG 1 cut(s) 75
PagI TCATGA 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 2 cut(s) 398, 569
PkrI GCNGC 2 cut(s) 525, 537
PspN4I GGNNCC 2 cut(s) 247, 428
PspXI VCTCGAGB 1 cut(s) 75
PsuI RGATCY 1 cut(s) 127
RsaI GTAC 1 cut(s) 462
RsaNI GTAC 1 cut(s) 461
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 2 cut(s) 524, 536
Sau3AI GATC 3 cut(s) 127, 211, 313
SfaNI GCATC 2 cut(s) 345, 378
Sfr274I CTCGAG 1 cut(s) 75
SlaI CTCGAG 1 cut(s) 75
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 2 cut(s) 29, 69
SsiI CCGC 2 cut(s) 239, 524
SspI AATATT 2 cut(s) 145, 201
SspMI CTAG 1 cut(s) 138
TaaI ACNGT 2 cut(s) 209, 267
TaiI ACGT 2 cut(s) 564, 620
TaqI TCGA 5 cut(s) 36, 76, 124, 204, 381
TasI AATT 2 cut(s) 29, 69
TauI GCSGC 1 cut(s) 526
TfiI GAWTC 2 cut(s) 398, 569
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 214
TseI GCWGC 1 cut(s) 535
TspDTI ATGAA 4 cut(s) 29, 33, 179, 422
TspRI CASTG 1 cut(s) 214
XceI RCATGY 1 cut(s) 484
XcmI CCANNNNNNNNNTGG 1 cut(s) 62
XhoI CTCGAG 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.