Rw5G023040

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000201
Physical Location & Seq
Reverse (-)
32426479 .. 32429120
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023040.1

Sequence Viewer

Length: 1761 bp
ATGGCCACTCTTTCTTTCACCTTCGTTGGAATTTACATGTCCCTTACTTTCTTCAACACTTCAACTGCAGCAGCAGCTGGTAGCACCTTGGTTTTTAAAAATGGCAGCTTTGAGTTGGGTTCTGTCAGTCCTCAAGATGCTATTTTTCAGAGGAGTCTTTTAGCATCAACTCCTCCGCGAGATATATGTGACACTGATGGCCGATGTGGCCCGAATGGACTGTGTGACATTAGTGACTCACAAGTTTGCAGTTGCTTAGAGGGATTCAAGCCCAAAGAAGAAGAGAACTGGAGCTTTGGGGAAAACTCGGATGGTTGTGTGCGTGTTACTCCATTGATGTGCCAAAGTAAGGATGATGGGTTTGTGAAATATGCCGGGGTCAAAGTGCCGGCGAGCATAGACTCTAGGGTGAACCAGAGCACGAGTCTCGAGGAATGCAAGGAGAGTTGCTTTAACAACTGTTCTTGTATGGCTTATGCAAGCTCTGATGTCAATGGCTGCACCATCTGGTTTGGTAATCTTTTCAACATTAGAAAGCTTCCACATGGAGGGAAGGATCTGTATGTTCGAGTGCCTACTTCCGAATTGAGCACGAAGCATCGATCTAAGTTGAAGATAATTATAGCGACAGTTGCATCTGGTGTTTGCATTGTTTTTGGGCTGCTCTTAGCCTTCTATTGCATTAGCAGAAAGAGGAGGAAACAGAGAGAGAAAATGGACAGAACGAACAACGGACAGAATGAAGACCTGGAGCTACCACTCTATAGTCTGTCGACAATTATAGATGCCACCGATAGCTTTTCATTCAACAAGAAGCTCGGGGAAGGTGGTTTTGGACCTGTATACAAGGGTAGACTAACAGATGGTCAAGAGATTGCCGTGAAGAGGCTCTCACAAACTTCTGGACAAGGTGCCACAGAGTTCAAAAATGAAGTGATACTTATAGCGAAACTTCAGCATCGAAATCTTGTAAAGCTCCTTGGTTGTTGCATTGAAGGGGAAGAGAAATTGCTGATCTATGAATATATGCCCAACAGAAGCTTGGACTGCTACATTTTCGATCAAACACAAGGTAGGCAGTTGGATTGGTCTCAGCGCTTCCACATTATTGAAGGTATAGCAAGGGGTCTTCTCTACTTGCATCAGGATTCCAGATTGAGGATTATTCATAGAGATCTTAAAGCAAGCAATGTTTTACTTGATGATGAGATGAAACCAAAAATCTCAGACTTTGGCATGGCTAGGCTTTTTGGAGGAGATGAGACTGAAGGAGTCACAAAAAGAGTAGTTGGAACCTACGGCTACATGGCACCAGAATATGCCATTGATGGTCAATTCTCTGTAAAATCCGACGTTTTTAGCTTTGGCATTTTATTGCTGGAGACATTGACCGGAAAGAGGAGTAGAGGGTTTCATGATCAGAAACACAACCTCAATCTAGTTGGATATGTGTGGAGAATGTGGAAAGAAGGAAGGTCCTTTGAGGCGATTGACGAATGCTTAAGGGAATCATGCACTCTATCAGAAGCATTGCATTGCGTACATGTTGGTCTTCTATGTGTCCAAGAGCTTCCTGAAGATAGGCCGACCATGTCAGCGGTGGTTCTGATGCTAGGTGGTGAGAGTGCCTTGCCTCAACCCAAAAGGCCAGGTTTATATGTAGGAAAATATTCATCATCTGAAGTATATTCATCTTCAAGTAAGAACGAAACATTTTCGAGTACTAATGAATGTTCAATAACTGTGGCGGAGGCCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

586

Amino Acids

65.08

Weight (kDa)

6.41

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 57 - 92 7.1e-09 S-locus glycoprotein domain
PAN_2 PF08276 114 - 177 3e-17 PAN-like domain
PK_Tyr_Ser-Thr PF07714 268 - 536 1.8e-49 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 268 - 534 6.3e-45 Protein kinase domain
DUF3403 PF11883 541 - 586 3.5e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 911, 1309
AccI GTMKAC 3 cut(s) 773, 843, 853
AccII CGCG 1 cut(s) 178
AciI CCGC 3 cut(s) 176, 1598, 1748
AclWI GGATC 1 cut(s) 564
AcoI YGGCCR 2 cut(s) 3, 199
AcsI RAATTY 1 cut(s) 30
AcuI CTGAAG 4 cut(s) 938, 1287, 1596, 1701
AfaI GTAC 2 cut(s) 1542, 1723
AfeI AGCGCT 1 cut(s) 1097
AfiI CCNNNNNNNGG 5 cut(s) 349, 548, 885, 1158, 1398
AflII CTTAAG 1 cut(s) 1501
AflIII ACRYGT 2 cut(s) 36, 1543
AjnI CCWGG 2 cut(s) 747, 1648
AjuI GAANNNNNNNTTGG 2 cut(s) 816, 848
Alw21I GWGCWC 2 cut(s) 422, 593
Alw26I GTCTC 4 cut(s) 431, 1095, 1256, 1376
AlwI GGATC 1 cut(s) 564
AlwNI CAGNNNCTG 1 cut(s) 77
Ama87I CYCGRG 2 cut(s) 428, 818
Aor51HI AGCGCT 1 cut(s) 1097
AoxI GGCC 6 cut(s) 3, 199, 208, 1583, 1646, 1752
ApeKI GCWGC 6 cut(s) 68, 71, 74, 105, 498, 661
ApoI RAATTY 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 1669
AspLEI GCGC 1 cut(s) 1098
AspS9I GGNCC 4 cut(s) 209, 836, 1476, 1753
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 3 cut(s) 10, 421, 1631
AvaI CYCGRG 2 cut(s) 428, 818
AvaII GGWCC 2 cut(s) 836, 1476
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 911, 1309
BauI CACGAG 1 cut(s) 421
BbsI GAAGAC 3 cut(s) 750, 1121, 1544
Bbv12I GWGCWC 2 cut(s) 422, 593
BbvI GCAGC 6 cut(s) 80, 83, 86, 117, 485, 648
BccI CCATC 6 cut(s) 191, 305, 350, 512, 857, 1322
BceAI ACGGC 2 cut(s) 863, 1315
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 2 cut(s) 749, 1650
BclI TGATCA 1 cut(s) 1417
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 4 cut(s) 431, 1095, 1256, 1376
BfaI CTAG 4 cut(s) 405, 1242, 1439, 1613
BfmI CTRYAG 2 cut(s) 66, 763
BfoI RGCGCY 1 cut(s) 1099
BfrI CTTAAG 1 cut(s) 1501
BglI GCCNNNNNGGC 1 cut(s) 207
BglII AGATCT 1 cut(s) 1174
BisI GCNGC 6 cut(s) 69, 72, 75, 106, 499, 662
BlsI GCNGC 6 cut(s) 70, 73, 76, 107, 500, 663
BmcAI AGTACT 1 cut(s) 1723
Bme1390I CCNGG 3 cut(s) 376, 749, 1650
Bme18I GGWCC 2 cut(s) 836, 1476
BmeT110I CYCGRG 2 cut(s) 428, 818
BmgT120I GGNCC 4 cut(s) 209, 836, 1476, 1753
BmiI GGNNCC 3 cut(s) 913, 1294, 1311
BmrFI CCNGG 3 cut(s) 376, 749, 1650
BmsI GCATC 8 cut(s) 127, 173, 607, 644, 775, 967, 1150, 1599
BpiI GAAGAC 3 cut(s) 750, 1121, 1544
BpmI CTGGAG 3 cut(s) 310, 770, 1400
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 376
Bsa29I ATCGAT 1 cut(s) 601
BsaI GGTCTC 1 cut(s) 1095
BsaJI CCNNGG 3 cut(s) 87, 375, 979
BsaWI WCCGGW 1 cut(s) 1391
Bsc4I CCNNNNNNNGG 5 cut(s) 349, 548, 885, 1158, 1398
Bse118I RCCGGY 1 cut(s) 388
Bse1I ACTGG 1 cut(s) 293
Bse3DI GCAATG 3 cut(s) 1195, 1529, 1534
BseBI CCWGG 2 cut(s) 749, 1650
BseCI ATCGAT 1 cut(s) 601
BseDI CCNNGG 3 cut(s) 87, 375, 979
BseGI GGATG 2 cut(s) 316, 358
BseLI CCNNNNNNNGG 5 cut(s) 349, 548, 885, 1158, 1398
BseMI GCAATG 3 cut(s) 1195, 1529, 1534
BseMII CTCAG 2 cut(s) 1106, 1239
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 5 cut(s) 162, 166, 709, 1269, 1414
BseXI GCAGC 6 cut(s) 80, 83, 86, 117, 485, 648
BsgI GTGCAG 1 cut(s) 484
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 6 cut(s) 5, 201, 210, 1585, 1648, 1754
BshNI GGYRCC 2 cut(s) 911, 1309
BshVI ATCGAT 1 cut(s) 601
BsiHKAI GWGCWC 2 cut(s) 422, 593
BsiHKCI CYCGRG 2 cut(s) 428, 818
BsiSI CCGG 3 cut(s) 375, 389, 1392
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 5 cut(s) 349, 548, 885, 1158, 1398
BsmAI GTCTC 4 cut(s) 431, 1095, 1256, 1376
BsmFI GGGAC 1 cut(s) 25
BsmI GAATGC 2 cut(s) 440, 1502
BsnI GGCC 6 cut(s) 5, 201, 210, 1585, 1648, 1754
Bso31I GGTCTC 1 cut(s) 1095
BsoBI CYCGRG 2 cut(s) 428, 818
Bsp1286I GDGCHC 2 cut(s) 422, 593
Bsp143I GATC 6 cut(s) 556, 602, 1014, 1060, 1174, 1417
BspACI CCGC 3 cut(s) 176, 1598, 1748
BspANI GGCC 6 cut(s) 5, 201, 210, 1585, 1648, 1754
BspCNI CTCAG 2 cut(s) 1105, 1238
BspDI ATCGAT 1 cut(s) 601
BspFNI CGCG 1 cut(s) 178
BspHI TCATGA 1 cut(s) 1414
BspLI GGNNCC 3 cut(s) 913, 1294, 1311
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 1 cut(s) 564
BspT107I GGYRCC 2 cut(s) 911, 1309
BspTI CTTAAG 1 cut(s) 1501
BspTNI GGTCTC 1 cut(s) 1095
BsrDI GCAATG 3 cut(s) 1195, 1529, 1534
BsrFI RCCGGY 1 cut(s) 388
BsrI ACTGG 1 cut(s) 293
BssAI RCCGGY 1 cut(s) 388
BssECI CCNNGG 3 cut(s) 87, 375, 979
BssMI GATC 6 cut(s) 556, 602, 1014, 1060, 1174, 1417
BssNAI GTATAC 1 cut(s) 844
BssSI CACGAG 1 cut(s) 421
BssT1I CCWWGG 2 cut(s) 87, 979
Bst1107I GTATAC 1 cut(s) 844
Bst2BI CACGAG 1 cut(s) 421
Bst2UI CCWGG 2 cut(s) 749, 1650
Bst4CI ACNGT 4 cut(s) 222, 461, 631, 1744
Bst6I CTCTTC 3 cut(s) 276, 878, 996
BstAFI CTTAAG 1 cut(s) 1501
BstC8I GCNNGC 4 cut(s) 390, 394, 481, 1186
BstDEI CTNAG 5 cut(s) 256, 606, 667, 1092, 1225
BstF5I GGATG 2 cut(s) 316, 358
BstFNI CGCG 1 cut(s) 178
BstH2I RGCGCY 1 cut(s) 1099
BstHHI GCGC 1 cut(s) 1098
BstKTI GATC 6 cut(s) 559, 605, 1017, 1063, 1177, 1420
BstMAI GTCTC 4 cut(s) 431, 1095, 1256, 1376
BstMBI GATC 6 cut(s) 556, 602, 1014, 1060, 1174, 1417
BstMWI GCNNNNNNNGC 4 cut(s) 74, 207, 632, 1047
BstNI CCWGG 2 cut(s) 749, 1650
BstNSI RCATGY 2 cut(s) 40, 1547
BstSCI CCNGG 3 cut(s) 374, 747, 1648
BstSFI CTRYAG 2 cut(s) 66, 763
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 6 cut(s) 80, 83, 86, 117, 485, 648
BstV2I GAAGAC 3 cut(s) 750, 1121, 1544
BstX2I RGATCY 2 cut(s) 556, 1174
BstYI RGATCY 2 cut(s) 556, 1174
BstZ17I GTATAC 1 cut(s) 844
Bsu15I ATCGAT 1 cut(s) 601
BsuRI GGCC 6 cut(s) 5, 201, 210, 1585, 1648, 1754
BsuTUI ATCGAT 1 cut(s) 601
BtsCI GGATG 2 cut(s) 316, 358
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 4 cut(s) 390, 394, 481, 1186
CaiI CAGNNNCTG 1 cut(s) 77
CciI TCATGA 1 cut(s) 1414
CfoI GCGC 1 cut(s) 1098
Cfr10I RCCGGY 1 cut(s) 388
Cfr13I GGNCC 4 cut(s) 209, 836, 1476, 1753
ClaI ATCGAT 1 cut(s) 601
Csp6I GTAC 2 cut(s) 1541, 1722
CspCI CAANNNNNGTGG 2 cut(s) 1725, 1760
CviAII CATG 8 cut(s) 37, 545, 1237, 1306, 1415, 1512, 1544, 1591
CviQI GTAC 2 cut(s) 1541, 1722
DdeI CTNAG 5 cut(s) 256, 606, 667, 1092, 1225
DpnI GATC 6 cut(s) 558, 604, 1016, 1062, 1176, 1419
DpnII GATC 6 cut(s) 556, 602, 1014, 1060, 1174, 1417
DraI TTTAAA 1 cut(s) 97
EaeI YGGCCR 2 cut(s) 3, 199
Eam1104I CTCTTC 3 cut(s) 276, 878, 996
EarI CTCTTC 3 cut(s) 276, 878, 996
Eco130I CCWWGG 2 cut(s) 87, 979
Eco31I GGTCTC 1 cut(s) 1095
Eco47I GGWCC 2 cut(s) 836, 1476
Eco47III AGCGCT 1 cut(s) 1097
Eco57I CTGAAG 4 cut(s) 938, 1287, 1596, 1701
Eco88I CYCGRG 2 cut(s) 428, 818
EcoO109I RGGNCCY 1 cut(s) 1476
EcoRII CCWGG 2 cut(s) 747, 1648
EcoT14I CCWWGG 2 cut(s) 87, 979
ErhI CCWWGG 2 cut(s) 87, 979
FaeI CATG 8 cut(s) 40, 548, 1240, 1309, 1418, 1515, 1547, 1594
FalI AAGNNNNNCTT 2 cut(s) 924, 956
FaqI GGGAC 1 cut(s) 25
FatI CATG 8 cut(s) 36, 544, 1236, 1305, 1414, 1511, 1543, 1590
FbaI TGATCA 1 cut(s) 1417
FblI GTMKAC 3 cut(s) 773, 843, 853
Fnu4HI GCNGC 6 cut(s) 69, 72, 75, 106, 499, 662
FokI GGATG 2 cut(s) 323, 365
Fsp4HI GCNGC 6 cut(s) 69, 72, 75, 106, 499, 662
FspBI CTAG 4 cut(s) 405, 1242, 1439, 1613
GlaI GCGC 1 cut(s) 1097
GluI GCNGC 6 cut(s) 69, 72, 75, 106, 499, 662
GsuI CTGGAG 3 cut(s) 310, 770, 1400
HaeII RGCGCY 1 cut(s) 1099
HaeIII GGCC 6 cut(s) 5, 201, 210, 1585, 1648, 1754
HapII CCGG 3 cut(s) 375, 389, 1392
HhaI GCGC 1 cut(s) 1098
Hin1II CATG 8 cut(s) 40, 548, 1240, 1309, 1418, 1515, 1547, 1594
Hin6I GCGC 1 cut(s) 1096
HinP1I GCGC 1 cut(s) 1096
HincII GTYRAC 1 cut(s) 774
HindII GTYRAC 1 cut(s) 774
HindIII AAGCTT 2 cut(s) 536, 1039
HinfI GANTC 8 cut(s) 154, 236, 264, 401, 424, 1148, 1272, 1508
HpaII CCGG 3 cut(s) 375, 389, 1392
HphI GGTGA 3 cut(s) 10, 421, 1631
Hpy166II GTNNAC 4 cut(s) 412, 774, 844, 854
Hpy188III TCNNGA 8 cut(s) 134, 428, 869, 903, 1145, 1152, 1415, 1574
Hpy8I GTNNAC 4 cut(s) 412, 774, 844, 854
Hpy99I CGWCG 1 cut(s) 1355
HpyAV CCTTC 9 cut(s) 31, 547, 682, 818, 989, 1106, 1262, 1463, 1467
HpyCH4III ACNGT 4 cut(s) 222, 461, 631, 1744
HpyCH4IV ACGT 1 cut(s) 1353
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 207, 632, 1047
HpyF3I CTNAG 5 cut(s) 256, 606, 667, 1092, 1225
HpySE526I ACGT 1 cut(s) 1353
Hsp92II CATG 8 cut(s) 40, 548, 1240, 1309, 1418, 1515, 1547, 1594
HspAI GCGC 1 cut(s) 1096
KroI GCCGGC 1 cut(s) 388
KroNI GCCGGC 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 1417
Kzo9I GATC 6 cut(s) 556, 602, 1014, 1060, 1174, 1417
LmnI GCTCC 3 cut(s) 291, 751, 981
Lsp1109I GCAGC 6 cut(s) 80, 83, 86, 117, 485, 648
LweI GCATC 8 cut(s) 127, 173, 607, 644, 775, 967, 1150, 1599
MaeI CTAG 4 cut(s) 405, 1242, 1439, 1613
MaeII ACGT 1 cut(s) 1353
MaeIII GTNAC 5 cut(s) 188, 224, 233, 325, 1273
MalI GATC 6 cut(s) 558, 604, 1016, 1062, 1176, 1419
MboI GATC 6 cut(s) 556, 602, 1014, 1060, 1174, 1417
MflI RGATCY 2 cut(s) 556, 1174
MhlI GDGCHC 2 cut(s) 422, 593
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 30, 584, 618, 777, 1007, 1334
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 5 cut(s) 163, 230, 395, 433, 1281
MmeI TCCRAC 5 cut(s) 7, 1062, 1270, 1374, 1423
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 388
MroXI GAANNNNTTC 1 cut(s) 1669
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 96, 453, 1179, 1502
MslI CAYNNNNRTG 1 cut(s) 337
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 77, 1598
MspCI CTTAAG 1 cut(s) 1501
MspI CCGG 3 cut(s) 375, 389, 1392
MspR9I CCNGG 3 cut(s) 376, 749, 1650
Mva1269I GAATGC 2 cut(s) 440, 1502
MvaI CCWGG 2 cut(s) 749, 1650
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 4 cut(s) 74, 207, 632, 1047
NaeI GCCGGC 1 cut(s) 390
NciI CCSGG 1 cut(s) 376
NdeII GATC 6 cut(s) 556, 602, 1014, 1060, 1174, 1417
NgoMIV GCCGGC 1 cut(s) 388
NlaIII CATG 8 cut(s) 40, 548, 1240, 1309, 1418, 1515, 1547, 1594
NlaIV GGNNCC 3 cut(s) 913, 1294, 1311
NmuCI GTSAC 4 cut(s) 188, 224, 233, 1273
NspI RCATGY 2 cut(s) 40, 1547
PaeR7I CTCGAG 1 cut(s) 428
PagI TCATGA 1 cut(s) 1414
PciI ACATGT 2 cut(s) 36, 1543
PctI GAATGC 2 cut(s) 440, 1502
PdiI GCCGGC 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 1669
PfeI GAWTC 3 cut(s) 264, 1148, 1508
PflFI GACNNNGTC 1 cut(s) 1591
PkrI GCNGC 6 cut(s) 70, 73, 76, 107, 500, 663
PleI GAGTC 5 cut(s) 162, 230, 395, 432, 1280
PpsI GAGTC 5 cut(s) 162, 230, 395, 432, 1280
PpuMI RGGWCCY 1 cut(s) 1476
PscI ACATGT 2 cut(s) 36, 1543
Psp5II RGGWCCY 1 cut(s) 1476
Psp6I CCWGG 2 cut(s) 747, 1648
PspGI CCWGG 2 cut(s) 747, 1648
PspN4I GGNNCC 3 cut(s) 913, 1294, 1311
PspPI GGNCC 4 cut(s) 209, 836, 1476, 1753
PspPPI RGGWCCY 1 cut(s) 1476
PstI CTGCAG 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 77
PsuI RGATCY 2 cut(s) 556, 1174
PsyI GACNNNGTC 1 cut(s) 1591
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 2 cut(s) 1542, 1723
RsaNI GTAC 2 cut(s) 1541, 1722
RseI CAYNNNNRTG 1 cut(s) 337
SalI GTCGAC 1 cut(s) 772
SaqAI TTAA 4 cut(s) 96, 453, 1179, 1502
SatI GCNGC 6 cut(s) 69, 72, 75, 106, 499, 662
Sau3AI GATC 6 cut(s) 556, 602, 1014, 1060, 1174, 1417
Sau96I GGNCC 4 cut(s) 209, 836, 1476, 1753
ScaI AGTACT 1 cut(s) 1723
SchI GAGTC 5 cut(s) 163, 230, 395, 433, 1281
ScrFI CCNGG 3 cut(s) 376, 749, 1650
SduI GDGCHC 2 cut(s) 422, 593
SfaNI GCATC 8 cut(s) 127, 173, 607, 644, 775, 967, 1150, 1599
SfcI CTRYAG 2 cut(s) 66, 763
SfiI GGCCNNNNNGGCC 1 cut(s) 207
Sfr274I CTCGAG 1 cut(s) 428
SinI GGWCC 2 cut(s) 836, 1476
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 337
SmlI CTYRAG 3 cut(s) 132, 428, 1501
SmoI CTYRAG 3 cut(s) 132, 428, 1501
Sse9I AATT 6 cut(s) 30, 584, 618, 777, 1007, 1334
SsiI CCGC 3 cut(s) 176, 1598, 1748
SspI AATATT 1 cut(s) 1670
SspMI CTAG 4 cut(s) 405, 1242, 1439, 1613
StyD4I CCNGG 3 cut(s) 374, 747, 1648
StyI CCWWGG 2 cut(s) 87, 979
TaaI ACNGT 4 cut(s) 222, 461, 631, 1744
TaiI ACGT 1 cut(s) 1356
TaqI TCGA 7 cut(s) 429, 568, 601, 773, 961, 1059, 1718
TasI AATT 6 cut(s) 30, 584, 618, 777, 1007, 1334
TatI WGTACW 1 cut(s) 1721
TfiI GAWTC 3 cut(s) 264, 1148, 1508
Tru1I TTAA 4 cut(s) 96, 453, 1179, 1502
Tru9I TTAA 4 cut(s) 96, 453, 1179, 1502
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 4 cut(s) 188, 224, 233, 1273
TseI GCWGC 6 cut(s) 68, 71, 74, 105, 498, 661
Tsp45I GTSAC 4 cut(s) 188, 224, 233, 1273
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 1 cut(s) 199
Tth111I GACNNNGTC 1 cut(s) 1591
Vha464I CTTAAG 1 cut(s) 1501
VpaK11BI GGWCC 2 cut(s) 836, 1476
XapI RAATTY 1 cut(s) 30
XceI RCATGY 2 cut(s) 40, 1547
XcmI CCANNNNNNNNNTGG 2 cut(s) 1039, 1597
XhoI CTCGAG 1 cut(s) 428
XmiI GTMKAC 3 cut(s) 773, 843, 853
XmnI GAANNNNTTC 1 cut(s) 1669
XspI CTAG 4 cut(s) 405, 1242, 1439, 1613
ZrmI AGTACT 1 cut(s) 1723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.