Rw5G023030

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000201
Physical Location & Seq
Reverse (-)
32387847 .. 32391756
3910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023030.1

Sequence Viewer

Length: 1734 bp
ATGGCCACTCTTTCTTTCACCTTCATTGGAATTTACTTGTCCCTTTCTTTCTTCAAGACTTCAACTGCAGCAGCAGCTGGTAGCACCTTGGTTTTCAAAAATGGCAGCTTTGAGTTGGGTTCTTTCAGTCCTCAAGATGCTATTTTTCGTAGGAGTCTTTTAGTATCAACTCCTCCGGGAGATGTATGTGACACTGATGGCCGATGTGGCCCGAATGGACTGTGTGACATTAGTGACTCACAAGTTTGCAGTTGCTTAGAGGGATTCAAGCCCAAAGAAGAAGAGAACTGGAGCTTTGGGGAAAACTCGGATGGTTGTGTGCGTGTTACTCCATTGATGTGCCAAAGTAAGGATGATGGGTTTGTGAAATATGCCGGGGTCAAAGTGCTGGCGAGCATAGACTCTAGGGTGAACCGGGGCACGAGTCTCGAGGAATGCAAGGAGAATTGCTTTAACAACTATTCTTGTATGGCTTATGCAAGCTCTGATGTCAGTGGCTGCACCATCTGGTTTGGTGATCTTTTCAACATTAGAAAGCTTCCACATGGAGGGCAGGATCTGTATGTTCGAGTGCTTACTTCCGAATCGAGCACGAAGCGTCGATCTAAGTTGAAGATAATTATAGCGATAGTTGCATCTGGTTTTTGCATCGTTTTTGGAATGCCCTTAGCCTTCTATTGCATTAGCAGAAAGAGGAGGAAACAGAAAGAGAAAATGGGAAAGTATGGAGGCATGGGTCAGAACAATGGACAGAATGAAGACCTGGAGCTACCACTATATAGTTTGTCGACAATTATAGATGCCACCGATTCATTCAACAAGAAGCTCGGGGAGGGTGGTTTTGGACCTGTGTACAAGGGTAGACTAACAGATGGTCAAGAGATTGCCGTGAAGAGGCTCTCACAAACTTCTGGACAAGGTGCCACAGAGTTCAAAAATGAAGTGATACTTATAGCGAAACTTCAGCATCGAAATCTTGTAAAGCTCCTTGGTTGTTGCATTGAAGGGGAAGAGAAATTGCTGAAATATGAATATATGCCCAACAGAAGCTTGGACTGCTACATTTTCGATCAAACACAAGGTCAGTTGGATTGGTCTCAGCGCTTCCACATTATCGAAGGTATAGCAAGGGGTCTTCTTTTCTTGCATCAGGATTCCAGACTGAGGATTATTCATAGAGATCTTAAAGCAAGCAATGTGTTACTTGATGATGAGATGAAACCAAAAATCTCAGACTTTGGCATGGCTAGGCTTTTTGGAGGAGATGAGACTGAAGGAGTCACAAAAAGAGTAGTTGGAACCTACGGTTACATGGCACCAGAATATGCCATTAATGGTCAATTCTCTGTAAAATCCGACGTTTTGAGCTTTGGCATTTTATTGCTGGAGACATTAACCGGAAAGAGGAGTAGAGGGTTTCATGATCAGAAACACAACCTTAATCTAGTTGGACATGTCTGGAGATTGTGGAAAGAAGGAAGGTCCTTTGAGGCGATTGACGAATGCTTAAGGGAATCATGTACTCTATCAGAAGCATTGCTTTGCATACATGTTGGTCTTCTATGTGTCCAAAAGCTTCCTGAAGATAGGCCGACCATTGCCTTGCCTCAACCCAAAAGGCCAGGTTTATATGTAGGAAAATATTCATCATCTGAAGTAGATTCATCTTCAAGTAAGAACGAAACATTCTCGTGTACAAATGAATGTTCGATAACTGTGGCGGAGGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

577

Amino Acids

64.02

Weight (kDa)

7.71

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 57 - 92 1.6e-08 S-locus glycoprotein domain
PAN_2 PF08276 114 - 177 6.6e-14 PAN-like domain
PK_Tyr_Ser-Thr PF07714 272 - 503 9.9e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 273 - 532 2e-42 Protein kinase domain
DUF3403 PF11883 532 - 577 7.3e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 920, 1315
AccI GTMKAC 2 cut(s) 788, 862
AciI CCGC 1 cut(s) 1721
AclWI GGATC 1 cut(s) 564
AcoI YGGCCR 2 cut(s) 3, 199
AcsI RAATTY 1 cut(s) 30
AcuI CTGAAG 4 cut(s) 947, 1293, 1602, 1674
AfaI GTAC 3 cut(s) 854, 1522, 1696
AfeI AGCGCT 1 cut(s) 1103
AfiI CCNNNNNNNGG 5 cut(s) 349, 548, 894, 1164, 1404
AflII CTTAAG 1 cut(s) 1507
AflIII ACRYGT 2 cut(s) 1453, 1549
AjnI CCWGG 2 cut(s) 762, 1621
Alw21I GWGCWC 1 cut(s) 593
Alw26I GTCTC 4 cut(s) 431, 1101, 1262, 1382
AlwI GGATC 1 cut(s) 564
AlwNI CAGNNNCTG 3 cut(s) 77, 498, 559
Ama87I CYCGRG 2 cut(s) 428, 827
Aor51HI AGCGCT 1 cut(s) 1103
AoxI GGCC 5 cut(s) 3, 199, 208, 1589, 1619
ApeKI GCWGC 5 cut(s) 68, 71, 74, 105, 498
ApoI RAATTY 1 cut(s) 30
ArsI GACNNNNNNTTYG 2 cut(s) 1066, 1098
AseI ATTAAT 1 cut(s) 1332
Asp700I GAANNNNTTC 1 cut(s) 1642
AspLEI GCGC 1 cut(s) 1104
AspS9I GGNCC 3 cut(s) 209, 845, 1482
AsuC2I CCSGG 3 cut(s) 177, 376, 416
AsuHPI GGTGA 3 cut(s) 10, 421, 527
AvaI CYCGRG 2 cut(s) 428, 827
AvaII GGWCC 2 cut(s) 845, 1482
BaeGI GKGCMC 1 cut(s) 422
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 920, 1315
BauI CACGAG 2 cut(s) 421, 1690
BbsI GAAGAC 3 cut(s) 765, 1127, 1550
Bbv12I GWGCWC 1 cut(s) 593
BbvI GCAGC 5 cut(s) 80, 83, 86, 117, 485
BccI CCATC 5 cut(s) 191, 305, 350, 512, 866
BceAI ACGGC 1 cut(s) 872
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 2 cut(s) 764, 1623
BclI TGATCA 1 cut(s) 1423
BcnI CCSGG 3 cut(s) 177, 376, 416
BcoDI GTCTC 4 cut(s) 431, 1101, 1262, 1382
BfaI CTAG 3 cut(s) 405, 1248, 1445
BfmI CTRYAG 1 cut(s) 66
BfoI RGCGCY 1 cut(s) 1105
BfrI CTTAAG 1 cut(s) 1507
BglI GCCNNNNNGGC 1 cut(s) 207
BglII AGATCT 1 cut(s) 1180
BisI GCNGC 5 cut(s) 69, 72, 75, 106, 499
BlsI GCNGC 5 cut(s) 70, 73, 76, 107, 500
Bme1390I CCNGG 5 cut(s) 177, 376, 416, 764, 1623
Bme18I GGWCC 2 cut(s) 845, 1482
BmeT110I CYCGRG 2 cut(s) 428, 827
BmgT120I GGNCC 3 cut(s) 209, 845, 1482
BmiI GGNNCC 3 cut(s) 922, 1300, 1317
BmrFI CCNGG 5 cut(s) 177, 376, 416, 764, 1623
BmsI GCATC 6 cut(s) 127, 644, 657, 790, 976, 1156
BpiI GAAGAC 3 cut(s) 765, 1127, 1550
BpmI CTGGAG 4 cut(s) 310, 785, 1406, 1480
Bpu10I CCTNAGC 1 cut(s) 667
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 3 cut(s) 177, 376, 416
BsaI GGTCTC 1 cut(s) 1101
BsaJI CCNNGG 4 cut(s) 87, 375, 415, 988
BsaWI WCCGGW 1 cut(s) 1397
Bsc4I CCNNNNNNNGG 5 cut(s) 349, 548, 894, 1164, 1404
Bse1I ACTGG 1 cut(s) 293
Bse3DI GCAATG 3 cut(s) 1201, 1535, 1596
BseBI CCWGG 2 cut(s) 764, 1623
BseDI CCNNGG 4 cut(s) 87, 375, 415, 988
BseGI GGATG 2 cut(s) 316, 358
BseLI CCNNNNNNNGG 5 cut(s) 349, 548, 894, 1164, 1404
BseMI GCAATG 3 cut(s) 1201, 1535, 1596
BseMII CTCAG 3 cut(s) 1112, 1154, 1245
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 4 cut(s) 162, 709, 1275, 1420
BseSI GKGCMC 1 cut(s) 422
BseXI GCAGC 5 cut(s) 80, 83, 86, 117, 485
BsgI GTGCAG 1 cut(s) 484
BshFI GGCC 5 cut(s) 5, 201, 210, 1591, 1621
BshNI GGYRCC 2 cut(s) 920, 1315
BsiHKAI GWGCWC 1 cut(s) 593
BsiHKCI CYCGRG 2 cut(s) 428, 827
BsiSI CCGG 4 cut(s) 176, 375, 415, 1398
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 5 cut(s) 349, 548, 894, 1164, 1404
BsmAI GTCTC 4 cut(s) 431, 1101, 1262, 1382
BsmFI GGGAC 1 cut(s) 25
BsmI GAATGC 3 cut(s) 440, 666, 1508
BsnI GGCC 5 cut(s) 5, 201, 210, 1591, 1621
Bso31I GGTCTC 1 cut(s) 1101
BsoBI CYCGRG 2 cut(s) 428, 827
Bsp1286I GDGCHC 2 cut(s) 422, 593
Bsp1407I TGTACA 2 cut(s) 852, 1694
Bsp143I GATC 6 cut(s) 517, 556, 602, 1069, 1180, 1423
BspACI CCGC 1 cut(s) 1721
BspANI GGCC 5 cut(s) 5, 201, 210, 1591, 1621
BspCNI CTCAG 3 cut(s) 1111, 1155, 1244
BspHI TCATGA 1 cut(s) 1420
BspLI GGNNCC 3 cut(s) 922, 1300, 1317
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 1 cut(s) 564
BspT107I GGYRCC 2 cut(s) 920, 1315
BspTI CTTAAG 1 cut(s) 1507
BspTNI GGTCTC 1 cut(s) 1101
BsrDI GCAATG 3 cut(s) 1201, 1535, 1596
BsrGI TGTACA 2 cut(s) 852, 1694
BsrI ACTGG 1 cut(s) 293
BssECI CCNNGG 4 cut(s) 87, 375, 415, 988
BssMI GATC 6 cut(s) 517, 556, 602, 1069, 1180, 1423
BssSI CACGAG 2 cut(s) 421, 1690
BssT1I CCWWGG 2 cut(s) 87, 988
Bst2BI CACGAG 2 cut(s) 421, 1690
Bst2UI CCWGG 2 cut(s) 764, 1623
Bst4CI ACNGT 3 cut(s) 222, 1307, 1717
Bst6I CTCTTC 3 cut(s) 276, 887, 1005
BstAFI CTTAAG 1 cut(s) 1507
BstAUI TGTACA 2 cut(s) 852, 1694
BstC8I GCNNGC 4 cut(s) 390, 394, 481, 1192
BstDEI CTNAG 6 cut(s) 256, 606, 667, 1098, 1163, 1231
BstF5I GGATG 2 cut(s) 316, 358
BstH2I RGCGCY 1 cut(s) 1105
BstHHI GCGC 1 cut(s) 1104
BstKTI GATC 6 cut(s) 520, 559, 605, 1072, 1183, 1426
BstMAI GTCTC 4 cut(s) 431, 1101, 1262, 1382
BstMBI GATC 6 cut(s) 517, 556, 602, 1069, 1180, 1423
BstMWI GCNNNNNNNGC 4 cut(s) 74, 207, 632, 1056
BstNI CCWGG 2 cut(s) 764, 1623
BstNSI RCATGY 2 cut(s) 1457, 1553
BstSCI CCNGG 5 cut(s) 175, 374, 414, 762, 1621
BstSFI CTRYAG 1 cut(s) 66
BstSLI GKGCMC 1 cut(s) 422
BstV1I GCAGC 5 cut(s) 80, 83, 86, 117, 485
BstV2I GAAGAC 3 cut(s) 765, 1127, 1550
BstX2I RGATCY 2 cut(s) 556, 1180
BstYI RGATCY 2 cut(s) 556, 1180
BsuRI GGCC 5 cut(s) 5, 201, 210, 1591, 1621
BtsCI GGATG 2 cut(s) 316, 358
BtsIMutI CAGTG 2 cut(s) 192, 499
Cac8I GCNNGC 4 cut(s) 390, 394, 481, 1192
CaiI CAGNNNCTG 3 cut(s) 77, 498, 559
CciI TCATGA 1 cut(s) 1420
CfoI GCGC 1 cut(s) 1104
Cfr13I GGNCC 3 cut(s) 209, 845, 1482
CseI GACGC 1 cut(s) 587
Csp6I GTAC 3 cut(s) 853, 1521, 1695
CviAII CATG 8 cut(s) 545, 733, 1243, 1312, 1421, 1454, 1518, 1550
CviQI GTAC 3 cut(s) 853, 1521, 1695
DdeI CTNAG 6 cut(s) 256, 606, 667, 1098, 1163, 1231
DpnI GATC 6 cut(s) 519, 558, 604, 1071, 1182, 1425
DpnII GATC 6 cut(s) 517, 556, 602, 1069, 1180, 1423
EaeI YGGCCR 2 cut(s) 3, 199
Eam1104I CTCTTC 3 cut(s) 276, 887, 1005
EarI CTCTTC 3 cut(s) 276, 887, 1005
Eco130I CCWWGG 2 cut(s) 87, 988
Eco31I GGTCTC 1 cut(s) 1101
Eco47I GGWCC 2 cut(s) 845, 1482
Eco47III AGCGCT 1 cut(s) 1103
Eco57I CTGAAG 4 cut(s) 947, 1293, 1602, 1674
Eco88I CYCGRG 2 cut(s) 428, 827
EcoO109I RGGNCCY 1 cut(s) 1482
EcoRII CCWGG 2 cut(s) 762, 1621
EcoT14I CCWWGG 2 cut(s) 87, 988
ErhI CCWWGG 2 cut(s) 87, 988
FaeI CATG 8 cut(s) 548, 736, 1246, 1315, 1424, 1457, 1521, 1553
FalI AAGNNNNNCTT 4 cut(s) 933, 965, 1524, 1556
FaqI GGGAC 1 cut(s) 25
FatI CATG 8 cut(s) 544, 732, 1242, 1311, 1420, 1453, 1517, 1549
FbaI TGATCA 1 cut(s) 1423
FblI GTMKAC 2 cut(s) 788, 862
Fnu4HI GCNGC 5 cut(s) 69, 72, 75, 106, 499
FokI GGATG 2 cut(s) 323, 365
Fsp4HI GCNGC 5 cut(s) 69, 72, 75, 106, 499
FspBI CTAG 3 cut(s) 405, 1248, 1445
GlaI GCGC 1 cut(s) 1103
GluI GCNGC 5 cut(s) 69, 72, 75, 106, 499
GsuI CTGGAG 4 cut(s) 310, 785, 1406, 1480
HaeII RGCGCY 1 cut(s) 1105
HaeIII GGCC 5 cut(s) 5, 201, 210, 1591, 1621
HapII CCGG 4 cut(s) 176, 375, 415, 1398
HgaI GACGC 1 cut(s) 587
HhaI GCGC 1 cut(s) 1104
Hin1II CATG 8 cut(s) 548, 736, 1246, 1315, 1424, 1457, 1521, 1553
Hin6I GCGC 1 cut(s) 1102
HinP1I GCGC 1 cut(s) 1102
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HindIII AAGCTT 3 cut(s) 536, 1048, 1574
HpaII CCGG 4 cut(s) 176, 375, 415, 1398
HphI GGTGA 3 cut(s) 10, 421, 527
Hpy166II GTNNAC 5 cut(s) 412, 789, 853, 863, 1695
Hpy188I TCNGA 9 cut(s) 310, 487, 583, 741, 1234, 1357, 1428, 1531, 1654
Hpy8I GTNNAC 5 cut(s) 412, 789, 853, 863, 1695
Hpy99I CGWCG 2 cut(s) 603, 1361
HpyAV CCTTC 7 cut(s) 31, 682, 998, 1112, 1268, 1469, 1473
HpyCH4III ACNGT 3 cut(s) 222, 1307, 1717
HpyCH4IV ACGT 1 cut(s) 1359
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 207, 632, 1056
HpyF3I CTNAG 6 cut(s) 256, 606, 667, 1098, 1163, 1231
HpySE526I ACGT 1 cut(s) 1359
Hsp92II CATG 8 cut(s) 548, 736, 1246, 1315, 1424, 1457, 1521, 1553
HspAI GCGC 1 cut(s) 1102
Ksp22I TGATCA 1 cut(s) 1423
Kzo9I GATC 6 cut(s) 517, 556, 602, 1069, 1180, 1423
LmnI GCTCC 3 cut(s) 291, 766, 990
Lsp1109I GCAGC 5 cut(s) 80, 83, 86, 117, 485
LweI GCATC 6 cut(s) 127, 644, 657, 790, 976, 1156
MaeI CTAG 3 cut(s) 405, 1248, 1445
MaeII ACGT 1 cut(s) 1359
MaeIII GTNAC 7 cut(s) 188, 224, 233, 325, 1200, 1279, 1307
MalI GATC 6 cut(s) 519, 558, 604, 1071, 1182, 1425
MboI GATC 6 cut(s) 517, 556, 602, 1069, 1180, 1423
MflI RGATCY 2 cut(s) 556, 1180
MhlI GDGCHC 2 cut(s) 422, 593
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 30, 445, 618, 792, 1016, 1340
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 5 cut(s) 163, 230, 395, 433, 1287
MmeI TCCRAC 4 cut(s) 1068, 1276, 1380, 1429
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 1642
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 453, 1185, 1332, 1394, 1440, 1508
MslI CAYNNNNRTG 2 cut(s) 337, 1690
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 77
MspCI CTTAAG 1 cut(s) 1507
MspI CCGG 4 cut(s) 176, 375, 415, 1398
MspR9I CCNGG 5 cut(s) 177, 376, 416, 764, 1623
Mva1269I GAATGC 3 cut(s) 440, 666, 1508
MvaI CCWGG 2 cut(s) 764, 1623
MwoI GCNNNNNNNGC 4 cut(s) 74, 207, 632, 1056
NciI CCSGG 3 cut(s) 177, 376, 416
NdeII GATC 6 cut(s) 517, 556, 602, 1069, 1180, 1423
NlaIII CATG 8 cut(s) 548, 736, 1246, 1315, 1424, 1457, 1521, 1553
NlaIV GGNNCC 3 cut(s) 922, 1300, 1317
NmuCI GTSAC 4 cut(s) 188, 224, 233, 1279
NspI RCATGY 2 cut(s) 1457, 1553
PaeR7I CTCGAG 1 cut(s) 428
PagI TCATGA 1 cut(s) 1420
PciI ACATGT 2 cut(s) 1453, 1549
PctI GAATGC 3 cut(s) 440, 666, 1508
PdmI GAANNNNTTC 1 cut(s) 1642
PfeI GAWTC 6 cut(s) 264, 584, 809, 1154, 1514, 1661
PfoI TCCNGGA 1 cut(s) 175
PkrI GCNGC 5 cut(s) 70, 73, 76, 107, 500
PleI GAGTC 5 cut(s) 162, 230, 395, 432, 1286
PpsI GAGTC 5 cut(s) 162, 230, 395, 432, 1286
PpuMI RGGWCCY 1 cut(s) 1482
PscI ACATGT 2 cut(s) 1453, 1549
PshBI ATTAAT 1 cut(s) 1332
Psp5II RGGWCCY 1 cut(s) 1482
Psp6I CCWGG 2 cut(s) 762, 1621
PspGI CCWGG 2 cut(s) 762, 1621
PspN4I GGNNCC 3 cut(s) 922, 1300, 1317
PspPI GGNCC 3 cut(s) 209, 845, 1482
PspPPI RGGWCCY 1 cut(s) 1482
PstI CTGCAG 1 cut(s) 70
PstNI CAGNNNCTG 3 cut(s) 77, 498, 559
PsuI RGATCY 2 cut(s) 556, 1180
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 3 cut(s) 854, 1522, 1696
RsaNI GTAC 3 cut(s) 853, 1521, 1695
RseI CAYNNNNRTG 2 cut(s) 337, 1690
SalI GTCGAC 1 cut(s) 787
SaqAI TTAA 6 cut(s) 453, 1185, 1332, 1394, 1440, 1508
SatI GCNGC 5 cut(s) 69, 72, 75, 106, 499
Sau3AI GATC 6 cut(s) 517, 556, 602, 1069, 1180, 1423
Sau96I GGNCC 3 cut(s) 209, 845, 1482
SchI GAGTC 5 cut(s) 163, 230, 395, 433, 1287
ScrFI CCNGG 5 cut(s) 177, 376, 416, 764, 1623
SduI GDGCHC 2 cut(s) 422, 593
SfaNI GCATC 6 cut(s) 127, 644, 657, 790, 976, 1156
SfcI CTRYAG 1 cut(s) 66
SfiI GGCCNNNNNGGCC 1 cut(s) 207
Sfr274I CTCGAG 1 cut(s) 428
SinI GGWCC 2 cut(s) 845, 1482
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 2 cut(s) 337, 1690
SmlI CTYRAG 3 cut(s) 132, 428, 1507
SmoI CTYRAG 3 cut(s) 132, 428, 1507
Sse9I AATT 6 cut(s) 30, 445, 618, 792, 1016, 1340
SsiI CCGC 1 cut(s) 1721
SspI AATATT 1 cut(s) 1643
SspMI CTAG 3 cut(s) 405, 1248, 1445
StyD4I CCNGG 5 cut(s) 175, 374, 414, 762, 1621
StyI CCWWGG 2 cut(s) 87, 988
TaaI ACNGT 3 cut(s) 222, 1307, 1717
TaiI ACGT 1 cut(s) 1362
TasI AATT 6 cut(s) 30, 445, 618, 792, 1016, 1340
TatI WGTACW 3 cut(s) 852, 1520, 1694
TfiI GAWTC 6 cut(s) 264, 584, 809, 1154, 1514, 1661
Tru1I TTAA 6 cut(s) 453, 1185, 1332, 1394, 1440, 1508
Tru9I TTAA 6 cut(s) 453, 1185, 1332, 1394, 1440, 1508
TscAI CASTG 2 cut(s) 199, 499
TseFI GTSAC 4 cut(s) 188, 224, 233, 1279
TseI GCWGC 5 cut(s) 68, 71, 74, 105, 498
Tsp45I GTSAC 4 cut(s) 188, 224, 233, 1279
TspRI CASTG 2 cut(s) 199, 499
Vha464I CTTAAG 1 cut(s) 1507
VpaK11BI GGWCC 2 cut(s) 845, 1482
VspI ATTAAT 1 cut(s) 1332
XapI RAATTY 1 cut(s) 30
XceI RCATGY 2 cut(s) 1457, 1553
XcmI CCANNNNNNNNNTGG 1 cut(s) 1048
XhoI CTCGAG 1 cut(s) 428
XmiI GTMKAC 2 cut(s) 788, 862
XmnI GAANNNNTTC 1 cut(s) 1642
XspI CTAG 3 cut(s) 405, 1248, 1445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.