MD05G1217400.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000201
Physical Location & Seq
Forward (+)
34811845 .. 34814663
2819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1217400.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGGAAGAGATGGAGTTACCACTGTTCGATTTGACCACCATAGCTAAAGCCACTAACAACTTTTCAAGCTGCAACAAGTTGGGAGAAGGTGGCTTTGGACCTGTGTACAAGGGTACACTGATAGGAGGAAAAGAAATTGCAGTAAAGAGGCTTTCAAAGAATTCCGGACAAGGAACGAGAGAGTTCAAAACTGAAGTTATACTCATAGCCAGACTTCAGCATCGTAATCTTGTTAAGCTTCTTGGTTGTTGCATTCAAAAAGACGAAAAAATTCTAATATATGAATTCATGCCCAACAAAAGCTTGGACTTCTATATTTTTGATCAGGAGGGACAAAAATTGCTTGATTGGCCTAAGTGCTTCCACATTATCGGTGGAGTTGCCCGAGGGCTTCTTTATCTTCACCAAGACTCTAGATTAAGGATTATACATCGAGATCTGAAACCTAGCAATATTCTACTAGATGATAATATGAACCCGAAGATTTCAGACTTCGGCCTGGCTAAAACATTCGGCGGTGAACAAAGTCATGCCAACACAAACAGAGTGGTTGGAACCTATGGTTATATGTCTCCGGAATATGCAGCAGATGGAATTTTTTCGATGAAATCTGATGTGTTTAGCTTTGGAGTCATACTGCTCGAGATGTTGAGTAGGAAGAAGAACAGGGGATTTCTTCATCCAGATCATGACCACAACCTTCTTGGACATGCATGGATACGATGGATTCAAGATAAACCACTAGAACTCATCCATAAGACATTTTGTGATTCGTGCAACATATCTGAAGTGGTAAGGTGTCTTCATGTGGGGCTGCTATGTGTGCAAAGAGTACCTGAGGATAGACCAAGCATGTCATCTGTGGTTCTGATGTTGAGCAGTGATGTTGCCTTGCCGCCTCCAAAGCAGCCCGGTTTTTACACTGAAAAAAGCGTCCCTGAATCATCATCAAGGACGCTTCTTTGTTCAAAGAATACTTTTAGCACTACGTTGATAGAACCTCGGATAAATAGCTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

341

Amino Acids

38.32

Weight (kDa)

8.49

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 23 - 288 9.2e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 25 - 289 8.9e-45 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 293 - 335 1e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 164, 574
AciI CCGC 2 cut(s) 516, 896
AcsI RAATTY 4 cut(s) 160, 270, 284, 594
AcuI CTGAAG 3 cut(s) 200, 213, 807
AfaI GTAC 3 cut(s) 107, 115, 834
AgsI TTSAA 7 cut(s) 66, 156, 187, 257, 731, 969, 1018
AjnI CCWGG 1 cut(s) 498
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 6 cut(s) 44, 69, 238, 303, 624, 1014
AluI AGCT 6 cut(s) 44, 69, 238, 303, 624, 1014
Alw26I GTCTC 1 cut(s) 576
Ama87I CYCGRG 2 cut(s) 384, 641
Aor13HI TCCGGA 2 cut(s) 164, 574
AoxI GGCC 2 cut(s) 350, 496
ApeKI GCWGC 4 cut(s) 69, 584, 814, 907
ApoI RAATTY 4 cut(s) 160, 270, 284, 594
Asp700I GAANNNNTTC 2 cut(s) 270, 598
AspS9I GGNCC 1 cut(s) 98
AsuC2I CCSGG 1 cut(s) 912
AsuHPI GGTGA 2 cut(s) 395, 530
AvaI CYCGRG 2 cut(s) 384, 641
AvaII GGWCC 1 cut(s) 98
AxyI CCTNAGG 1 cut(s) 837
BarI GAAGNNNNNNTAC 2 cut(s) 786, 818
BbsI GAAGAC 1 cut(s) 794
BbvI GCAGC 4 cut(s) 56, 596, 801, 919
BccI CCATC 3 cut(s) 4, 584, 717
BciT130I CCWGG 1 cut(s) 500
BciVI GTATCC 1 cut(s) 711
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 1 cut(s) 912
BcoDI GTCTC 1 cut(s) 576
BfaI CTAG 4 cut(s) 414, 447, 461, 743
BfuI GTATCC 1 cut(s) 711
BglII AGATCT 1 cut(s) 436
BisI GCNGC 5 cut(s) 70, 585, 815, 896, 908
BlsI GCNGC 5 cut(s) 71, 586, 816, 897, 909
Bme1390I CCNGG 2 cut(s) 500, 912
Bme18I GGWCC 1 cut(s) 98
BmeT110I CYCGRG 2 cut(s) 384, 641
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 2 cut(s) 500, 912
BmsI GCATC 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 912
BsaJI CCNNGG 2 cut(s) 385, 1001
BsaWI WCCGGW 2 cut(s) 164, 574
Bse21I CCTNAGG 1 cut(s) 837
BseAI TCCGGA 2 cut(s) 164, 574
BseBI CCWGG 1 cut(s) 500
BseDI CCNNGG 2 cut(s) 385, 1001
BseGI GGATG 2 cut(s) 679, 750
BseMII CTCAG 1 cut(s) 828
BseXI GCAGC 4 cut(s) 56, 596, 801, 919
BshFI GGCC 2 cut(s) 352, 498
BsiHKCI CYCGRG 2 cut(s) 384, 641
BsiSI CCGG 3 cut(s) 165, 575, 912
BslFI GGGAC 2 cut(s) 345, 920
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 2 cut(s) 345, 920
BsmI GAATGC 1 cut(s) 252
BsnI GGCC 2 cut(s) 352, 498
BsoBI CYCGRG 2 cut(s) 384, 641
Bsp13I TCCGGA 2 cut(s) 164, 574
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 3 cut(s) 322, 436, 685
BspACI CCGC 2 cut(s) 516, 896
BspANI GGCC 2 cut(s) 352, 498
BspCNI CTCAG 1 cut(s) 829
BspEI TCCGGA 2 cut(s) 164, 574
BspHI TCATGA 1 cut(s) 688
BspLI GGNNCC 1 cut(s) 556
BsrGI TGTACA 1 cut(s) 105
BssECI CCNNGG 2 cut(s) 385, 1001
BssMI GATC 3 cut(s) 322, 436, 685
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 1 cut(s) 24
BstAUI TGTACA 1 cut(s) 105
BstDEI CTNAG 2 cut(s) 354, 837
BstF5I GGATG 2 cut(s) 679, 750
BstKTI GATC 3 cut(s) 325, 439, 688
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 3 cut(s) 322, 436, 685
BstMWI GCNNNNNNNGC 3 cut(s) 349, 823, 904
BstNI CCWGG 1 cut(s) 500
BstNSI RCATGY 2 cut(s) 713, 856
BstSCI CCNGG 2 cut(s) 498, 910
BstV1I GCAGC 4 cut(s) 56, 596, 801, 919
BstV2I GAAGAC 1 cut(s) 794
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
Bsu36I CCTNAGG 1 cut(s) 837
BsuI GTATCC 1 cut(s) 711
BsuRI GGCC 2 cut(s) 352, 498
BtsCI GGATG 2 cut(s) 679, 750
BtsI GCAGTG 1 cut(s) 886
BtsIMutI CAGTG 4 cut(s) 20, 116, 886, 921
CciI TCATGA 1 cut(s) 688
Cfr13I GGNCC 1 cut(s) 98
CseI GACGC 2 cut(s) 922, 964
Csp6I GTAC 3 cut(s) 106, 114, 833
CspCI CAANNNNNGTGG 2 cut(s) 528, 563
CviAII CATG 7 cut(s) 289, 530, 689, 710, 714, 806, 853
CviQI GTAC 3 cut(s) 106, 114, 833
DdeI CTNAG 2 cut(s) 354, 837
DpnI GATC 3 cut(s) 324, 438, 687
DpnII GATC 3 cut(s) 322, 436, 685
Eco47I GGWCC 1 cut(s) 98
Eco57I CTGAAG 3 cut(s) 200, 213, 807
Eco81I CCTNAGG 1 cut(s) 837
Eco88I CYCGRG 2 cut(s) 384, 641
EcoRI GAATTC 2 cut(s) 160, 284
EcoRII CCWGG 1 cut(s) 498
EcoT22I ATGCAT 1 cut(s) 715
FaeI CATG 7 cut(s) 292, 533, 692, 713, 717, 809, 856
FaqI GGGAC 2 cut(s) 345, 920
FatI CATG 7 cut(s) 288, 529, 688, 709, 713, 805, 852
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 5 cut(s) 70, 585, 815, 896, 908
FokI GGATG 2 cut(s) 666, 737
Fsp4HI GCNGC 5 cut(s) 70, 585, 815, 896, 908
FspBI CTAG 4 cut(s) 414, 447, 461, 743
GluI GCNGC 5 cut(s) 70, 585, 815, 896, 908
HaeIII GGCC 2 cut(s) 352, 498
HapII CCGG 3 cut(s) 165, 575, 912
HgaI GACGC 2 cut(s) 922, 964
Hin1II CATG 7 cut(s) 292, 533, 692, 713, 717, 809, 856
HindIII AAGCTT 2 cut(s) 236, 301
HinfI GANTC 5 cut(s) 410, 630, 727, 770, 941
HpaII CCGG 3 cut(s) 165, 575, 912
HphI GGTGA 2 cut(s) 395, 530
Hpy166II GTNNAC 3 cut(s) 106, 116, 521
Hpy188I TCNGA 6 cut(s) 441, 490, 613, 787, 870, 1005
Hpy188III TCNNGA 9 cut(s) 165, 326, 414, 434, 575, 643, 683, 689, 731
Hpy8I GTNNAC 3 cut(s) 106, 116, 521
HpyAV CCTTC 2 cut(s) 80, 710
HpyCH4III ACNGT 1 cut(s) 24
HpyCH4IV ACGT 1 cut(s) 989
HpyCH4V TGCA 7 cut(s) 72, 140, 252, 584, 713, 777, 826
HpyF10VI GCNNNNNNNGC 3 cut(s) 349, 823, 904
HpyF3I CTNAG 2 cut(s) 354, 837
HpySE526I ACGT 1 cut(s) 989
Hsp92II CATG 7 cut(s) 292, 533, 692, 713, 717, 809, 856
Kpn2I TCCGGA 2 cut(s) 164, 574
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 3 cut(s) 322, 436, 685
Lsp1109I GCAGC 4 cut(s) 56, 596, 801, 919
LweI GCATC 1 cut(s) 229
MaeI CTAG 4 cut(s) 414, 447, 461, 743
MaeII ACGT 1 cut(s) 989
MaeIII GTNAC 1 cut(s) 15
MalI GATC 3 cut(s) 324, 438, 687
MboI GATC 3 cut(s) 322, 436, 685
MboII GAAGA 7 cut(s) 17, 392, 493, 668, 670, 673, 794
MflI RGATCY 1 cut(s) 436
MluCI AATT 6 cut(s) 135, 160, 270, 284, 338, 594
MlyI GAGTC 2 cut(s) 404, 639
MmeI TCCRAC 1 cut(s) 532
MnlI CCTC 7 cut(s) 119, 141, 322, 380, 832, 909, 1011
Mph1103I ATGCAT 1 cut(s) 715
MroI TCCGGA 2 cut(s) 164, 574
MroXI GAANNNNTTC 2 cut(s) 270, 598
MseI TTAA 2 cut(s) 234, 419
MspI CCGG 3 cut(s) 165, 575, 912
MspR9I CCNGG 2 cut(s) 500, 912
Mva1269I GAATGC 1 cut(s) 252
MvaI CCWGG 1 cut(s) 500
MwoI GCNNNNNNNGC 3 cut(s) 349, 823, 904
NciI CCSGG 1 cut(s) 912
NdeII GATC 3 cut(s) 322, 436, 685
NlaIII CATG 7 cut(s) 292, 533, 692, 713, 717, 809, 856
NlaIV GGNNCC 1 cut(s) 556
NsiI ATGCAT 1 cut(s) 715
NspI RCATGY 2 cut(s) 713, 856
PaeR7I CTCGAG 1 cut(s) 641
PagI TCATGA 1 cut(s) 688
PctI GAATGC 1 cut(s) 252
PdmI GAANNNNTTC 2 cut(s) 270, 598
PfeI GAWTC 3 cut(s) 727, 770, 941
PkrI GCNGC 5 cut(s) 71, 586, 816, 897, 909
PleI GAGTC 2 cut(s) 404, 638
PpsI GAGTC 2 cut(s) 404, 638
Psp6I CCWGG 1 cut(s) 498
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 1 cut(s) 98
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 3 cut(s) 107, 115, 834
RsaNI GTAC 3 cut(s) 106, 114, 833
SaqAI TTAA 2 cut(s) 234, 419
SatI GCNGC 5 cut(s) 70, 585, 815, 896, 908
Sau3AI GATC 3 cut(s) 322, 436, 685
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 2 cut(s) 404, 639
ScrFI CCNGG 2 cut(s) 500, 912
SfaNI GCATC 1 cut(s) 229
Sfr274I CTCGAG 1 cut(s) 641
SinI GGWCC 1 cut(s) 98
SlaI CTCGAG 1 cut(s) 641
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 6 cut(s) 135, 160, 270, 284, 338, 594
SsiI CCGC 2 cut(s) 516, 896
SspI AATATT 1 cut(s) 454
SspMI CTAG 4 cut(s) 414, 447, 461, 743
StyD4I CCNGG 2 cut(s) 498, 910
TaaI ACNGT 1 cut(s) 24
TaiI ACGT 1 cut(s) 992
TaqI TCGA 4 cut(s) 27, 433, 602, 642
TasI AATT 6 cut(s) 135, 160, 270, 284, 338, 594
TatI WGTACW 1 cut(s) 105
TauI GCSGC 1 cut(s) 898
TfiI GAWTC 3 cut(s) 727, 770, 941
Tru1I TTAA 2 cut(s) 234, 419
Tru9I TTAA 2 cut(s) 234, 419
TscAI CASTG 4 cut(s) 27, 123, 886, 928
TseI GCWGC 4 cut(s) 69, 584, 814, 907
TspDTI ATGAA 6 cut(s) 277, 297, 488, 620, 668, 794
TspRI CASTG 4 cut(s) 27, 123, 886, 928
VpaK11BI GGWCC 1 cut(s) 98
XapI RAATTY 4 cut(s) 160, 270, 284, 594
XbaI TCTAGA 1 cut(s) 413
XceI RCATGY 2 cut(s) 713, 856
XcmI CCANNNNNNNNNTGG 3 cut(s) 301, 371, 701
XhoI CTCGAG 1 cut(s) 641
XmnI GAANNNNTTC 2 cut(s) 270, 598
XspI CTAG 4 cut(s) 414, 447, 461, 743
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.