MD05G1232800.v1.1

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000201
Physical Location & Seq
Forward (+)
36555319 .. 36555887
569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1232800.v1.1.491

Sequence Viewer

Length: 480 bp
ATGTTTTTTGACACATTGGTTCTCAGCGGTTACATGTCCCCAGAATATGCAATTGATGGTTTCTACTCTATAAAGTCCGACGTCTATAGCTTTGGTGTTATGGTGCTAGAGATAGTGAGTGGGAGCAGAAATAGAGGATTCTCTCATCCAGACCACAAACTCAACCTTCTTGGACATGCATGGATGCTACACACAGAAGACATGCCTCTCAAACTGATTGATGCATCGGTAGAGGACTCCGTCACTTTGCATGAAGTTGTAAGAACGATTCATGTGGGTCTTTTATGTGTGCAGCAGAATCCAGAAGACAGGCCGAACATGTCAACTGCAGTTCTAATGTTGGGTGGTGAAGGTGCGTTGCCCCCACCTCTAAAACCTGGTTTTTACAGTGAAAGGGATCTGACTGAACTCGAAGTCAATCATTCTTCTAAAGCATGCTCAGCTAATGAAGTCACTATTTCACTAGTCGAGGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

160

Amino Acids

17.58

Weight (kDa)

4.99

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 11 - 110 7.8e-08 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 118 - 159 2e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21366
fragaria_vesca FvH4_3g15730 FvH4_3g21351 FvH4_3g21401 FvH4_3g21420
malus_domestica MD00G1151400.v1.1 MD03G1185300.v1.1 MD05G1217100.v1.1 MD05G1217400.v1.1 MD05G1220300.v1.1 MD05G1232800.v1.1 MD05G1332500.v1.1 MD05G1332800.v1.1 MD09G1059900.v1.1 MD10G1308300.v1.1 MD11G1231000.v1.1 MD12G1047500.v1.1 MD17G1273100.v1.1
prunus_persica Prupe.4G031700_v2.0.a1 Prupe.4G031700_v2.0.a1 Prupe.4G142200_v2.0.a1 Prupe.4G142700_v2.0.a1 Prupe.4G195200_v2.0.a1 Prupe.4G195600_v2.0.a1 Prupe.4G195800_v2.0.a1 Prupe.4G196000_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196200_v2.0.a1 Prupe.4G196400_v2.0.a1 Prupe.4G237800_v2.0.a1
pyrus_communis pycom03g16470 pycom03g16480 pycom03g16490 pycom03g16500 pycom03g16510 pycom05g19890 pycom05g20180 pycom05g20300 pycom05g30520 pycom09g02440 pycom11g20450
rosa_chinensis RchiOBHm_Chr1g0363501 RchiOBHm_Chr3g0477311 RchiOBHm_Chr3g0483581 RchiOBHm_Chr4g0411701 RchiOBHm_Chr5g0025991 RchiOBHm_Chr5g0026501 RchiOBHm_Chr5g0026541 RchiOBHm_Chr5g0026771 RchiOBHm_Chr5g0035631 RchiOBHm_Chr5g0035871 RchiOBHm_Chr5g0036451 RchiOBHm_Chr5g0036751 RchiOBHm_Chr5g0036771 RchiOBHm_Chr5g0036781 RchiOBHm_Chr5g0036801 RchiOBHm_Chr5g0036851
rosa_laevigata RLG00000031277 RLG00000032829 RLG00000032831 RLG00000032890 RLG00000032895 RLG00000032899 RLG00000032924 RLG00000033714 RLG00000033715 RLG00000033719 RLG00000033720 RLG00000033722 RLG00000033726
rosa_multiflora Rmu_co8109542.1_g000001 Rmu_co8147940.1_g000001 Rmu_co8162180.1_g000001 Rmu_co8193822.1_g000001 Rmu_co8362785.1_g000001 Rmu_co8410231.1_g000001 Rmu_co8422055.1_g000001 Rmu_sc0000149.1_g000048 Rmu_sc0000399.1_g000001 Rmu_sc0000555.1_g000013 Rmu_sc0000593.1_g000001 Rmu_sc0001348.1_g000035 Rmu_sc0004371.1_g000010 Rmu_sc0004371.1_g000019 Rmu_sc0005555.1_g000004 Rmu_sc0006059.1_g000074 Rmu_sc0006968.1_g000016 Rmu_sc0006968.1_g000018 Rmu_sc0006968.1_g000022 Rmu_sc0008732.1_g000004 Rmu_sc0010714.1_g000004 Rmu_sc0015364.1_g000001 Rmu_sc0015448.1_g000001 Rmu_sc0016543.1_g000005 Rmu_sc0025001.1_g000005
rosa_roxburghii Rroxscaffold_176G00431020 Rroxscaffold_1G00007210 Rroxscaffold_1G00044150 Rroxscaffold_1G00044170 Rroxscaffold_1G00044190 Rroxscaffold_1G00044220 Rroxscaffold_1G00044860 Rroxscaffold_1G00044870 Rroxscaffold_1G00052580 Rroxscaffold_1G00052940 Rroxscaffold_1G00053250 Rroxscaffold_1G00070620 Rroxscaffold_1G00070690 Rroxscaffold_1G00070720 Rroxscaffold_5G00353610 Rroxscaffold_6G00398200 Rroxscaffold_7G00204580
rosa_rugosa Rorug01G0307900 Rorug03G0207300 Rorug05G0086700 Rorug05G0156300 Rorug05G0157800 Rorug05G0157900 Rorug05G0157900 Rorug05G0157900
rosa_samantha Rh1BG280000 Rh3AG255400 Rh5AG189100 Rh5AG250500 Rh5BG252100 Rh5CG198600 Rh5CG203300 Rh5CG205400 Rh5CG206200 Rh5CG206600 Rh5CG281100 Rh5CG282300 Rh5CG283600 Rh5CG283800 Rh5CG284100 Rh5CG424200 Rh5DG181000 Rh5DG258000 Rh5DG260000 Rh5DG260400 Rh5DG260500
rosa_wichuraiana Rw1G028060 Rw3G023100 Rw5G016880 Rw5G017170 Rw5G022990 Rw5G023010 Rw5G023030 Rw5G023040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 84
AciI CCGC 1 cut(s) 27
AclWI GGATC 1 cut(s) 405
AcyI GRCGYC 1 cut(s) 81
AflIII ACRYGT 2 cut(s) 33, 318
AhlI ACTAGT 1 cut(s) 463
AjnI CCWGG 1 cut(s) 376
AluBI AGCT 2 cut(s) 90, 443
AluI AGCT 2 cut(s) 90, 443
AlwI GGATC 1 cut(s) 405
AoxI GGCC 1 cut(s) 311
ApeKI GCWGC 1 cut(s) 292
AsuHPI GGTGA 1 cut(s) 359
BbsI GAAGAC 2 cut(s) 204, 312
BbvI GCAGC 1 cut(s) 304
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 378
BcuI ACTAGT 1 cut(s) 463
BfaI CTAG 2 cut(s) 107, 464
BfmI CTRYAG 2 cut(s) 85, 327
BisI GCNGC 1 cut(s) 293
BlpI GCTNAGC 1 cut(s) 439
BlsI GCNGC 1 cut(s) 294
Bme1390I CCNGG 1 cut(s) 378
BmrFI CCNGG 1 cut(s) 378
BmsI GCATC 3 cut(s) 174, 211, 233
BpiI GAAGAC 2 cut(s) 204, 312
Bpu1102I GCTNAGC 1 cut(s) 439
BsaHI GRCGYC 1 cut(s) 81
BseBI CCWGG 1 cut(s) 378
BseGI GGATG 2 cut(s) 145, 189
BseMII CTCAG 2 cut(s) 37, 453
BseXI GCAGC 1 cut(s) 304
BsgI GTGCAG 1 cut(s) 311
BshFI GGCC 1 cut(s) 313
BslFI GGGAC 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 1 cut(s) 313
Bsp143I GATC 1 cut(s) 397
Bsp1720I GCTNAGC 1 cut(s) 439
BspACI CCGC 1 cut(s) 27
BspANI GGCC 1 cut(s) 313
BspCNI CTCAG 2 cut(s) 36, 452
BspMAI CTGCAG 1 cut(s) 331
BspPI GGATC 1 cut(s) 405
BssMI GATC 1 cut(s) 397
BssNI GRCGYC 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 389
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 436
BstDEI CTNAG 2 cut(s) 23, 439
BstF5I GGATG 2 cut(s) 145, 189
BstKTI GATC 1 cut(s) 400
BstMBI GATC 1 cut(s) 397
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 5 cut(s) 37, 179, 205, 322, 438
BstSCI CCNGG 1 cut(s) 376
BstSFI CTRYAG 2 cut(s) 85, 327
BstV1I GCAGC 1 cut(s) 304
BstV2I GAAGAC 2 cut(s) 204, 312
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
BsuRI GGCC 1 cut(s) 313
BtsCI GGATG 2 cut(s) 145, 189
BtsIMutI CAGTG 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 436
CsiI ACCWGGT 1 cut(s) 376
CviAII CATG 8 cut(s) 34, 176, 180, 202, 251, 272, 319, 435
CviJI RGCY 4 cut(s) 90, 313, 443, 473
CviKI_1 RGCY 4 cut(s) 90, 313, 443, 473
DdeI CTNAG 2 cut(s) 23, 439
DpnI GATC 1 cut(s) 399
DpnII GATC 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 376
EcoT22I ATGCAT 2 cut(s) 181, 226
FaeI CATG 8 cut(s) 37, 179, 183, 205, 254, 275, 322, 438
FaqI GGGAC 1 cut(s) 22
FatI CATG 8 cut(s) 33, 175, 179, 201, 250, 271, 318, 434
Fnu4HI GCNGC 1 cut(s) 293
FokI GGATG 2 cut(s) 132, 196
Fsp4HI GCNGC 1 cut(s) 293
FspBI CTAG 2 cut(s) 107, 464
GluI GCNGC 1 cut(s) 293
HaeIII GGCC 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 8 cut(s) 37, 179, 183, 205, 254, 275, 322, 438
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HinfI GANTC 4 cut(s) 138, 236, 268, 298
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 1 cut(s) 324
Hpy188I TCNGA 2 cut(s) 79, 402
Hpy188III TCNNGA 2 cut(s) 149, 302
Hpy8I GTNNAC 1 cut(s) 324
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 176, 344
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 6 cut(s) 50, 179, 224, 250, 292, 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpyF3I CTNAG 2 cut(s) 23, 439
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 8 cut(s) 37, 179, 183, 205, 254, 275, 322, 438
Kzo9I GATC 1 cut(s) 397
LmnI GCTCC 1 cut(s) 123
LpnPI CCDG 6 cut(s) 54, 162, 295, 315, 363, 390
Lsp1109I GCAGC 1 cut(s) 304
LweI GCATC 3 cut(s) 174, 211, 233
MabI ACCWGGT 1 cut(s) 376
MaeI CTAG 2 cut(s) 107, 464
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 3 cut(s) 29, 241, 451
MalI GATC 1 cut(s) 399
MboI GATC 1 cut(s) 397
MboII GAAGA 3 cut(s) 209, 317, 417
MfeI CAATTG 1 cut(s) 51
MflI RGATCY 1 cut(s) 397
MluCI AATT 1 cut(s) 51
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 5 cut(s) 128, 216, 226, 378, 463
Mph1103I ATGCAT 2 cut(s) 181, 226
MspA1I CMGCKG 1 cut(s) 27
MspR9I CCNGG 1 cut(s) 378
MunI CAATTG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 378
MwoI GCNNNNNNNGC 1 cut(s) 440
NdeII GATC 1 cut(s) 397
NlaIII CATG 8 cut(s) 37, 179, 183, 205, 254, 275, 322, 438
NmuCI GTSAC 2 cut(s) 241, 451
NsiI ATGCAT 2 cut(s) 181, 226
NspI RCATGY 5 cut(s) 37, 179, 205, 322, 438
PaeI GCATGC 1 cut(s) 438
PciI ACATGT 2 cut(s) 33, 318
PfeI GAWTC 3 cut(s) 138, 268, 298
PflFI GACNNNGTC 1 cut(s) 239
PkrI GCNGC 1 cut(s) 294
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PscI ACATGT 2 cut(s) 33, 318
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PstI CTGCAG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 397
PsyI GACNNNGTC 1 cut(s) 239
SatI GCNGC 1 cut(s) 293
Sau3AI GATC 1 cut(s) 397
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 1 cut(s) 378
SetI ASST 7 cut(s) 84, 92, 168, 355, 370, 379, 445
SexAI ACCWGGT 1 cut(s) 376
SfaNI GCATC 3 cut(s) 174, 211, 233
SfcI CTRYAG 2 cut(s) 85, 327
SpeI ACTAGT 1 cut(s) 463
SphI GCATGC 1 cut(s) 438
Sse9I AATT 1 cut(s) 51
SsiI CCGC 1 cut(s) 27
SspMI CTAG 2 cut(s) 107, 464
StyD4I CCNGG 1 cut(s) 376
TaaI ACNGT 1 cut(s) 389
TaiI ACGT 1 cut(s) 84
TaqI TCGA 3 cut(s) 411, 468, 475
TasI AATT 1 cut(s) 51
TfiI GAWTC 3 cut(s) 138, 268, 298
TscAI CASTG 1 cut(s) 394
TseFI GTSAC 2 cut(s) 241, 451
TseI GCWGC 1 cut(s) 292
Tsp45I GTSAC 2 cut(s) 241, 451
TspDTI ATGAA 3 cut(s) 260, 267, 462
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 1 cut(s) 394
Tth111I GACNNNGTC 1 cut(s) 239
XceI RCATGY 5 cut(s) 37, 179, 205, 322, 438
XspI CTAG 2 cut(s) 107, 464
ZraI GACGTC 1 cut(s) 82
Zsp2I ATGCAT 2 cut(s) 181, 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.