FvH4_1g25271

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000220
Physical Location & Seq
Reverse (-)
17066655 .. 17068069
1415 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25271.t1

Sequence Viewer

Length: 597 bp
ATGGAATTCAAAGGCATTTTTGAATATGTTGACACACTCAAGGCAACATGGGAGACCTACGGATGCACGATCATGTGCGATGAATGGACAAGGCCAACTAGATTGTCCATCATAAATTTCATGGTCTACTCCAAAGGTACCACTGTTTTTCTCAAGTCTGTTGATTTTTCTGGGGAGAAGAAAGAGCATGAGTATATATACAAATTGTTGAAGGGAGTTATAAGAGAGGTTAGAGAGCGCAATGTGGTTGAAGTGGTGACTGACAATGCTGCTAATTATGTCAACGCTGGAAGGAATGATGGACCATACCCTTACAGCCAAAATCTGGCAATGCCTTATCTAGTATATTCCATGCCTTATGGGGTGCAATCAAGTGATAACTCATGGTCACAGTCGGAAGGACAATCTTCCAATGATTATGGTTATGGCCAAGGTCAAGCGATGCCCAATCCCTACTATAACTTCCACTATCTTGAGGGTGCAGCGTGGTCCATATATGGTTTTGGAGACCTCGACAAACGAGGGTGTGGTGTGGATCTTGAAACCAATGGGAAGTTGGCTTTGGTGTCCTTTGAACTGGCCTCTTCATCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

199

Amino Acids

22.39

Weight (kDa)

4.88

Isoelectric Point (pI)

20.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 5 - 98 2.1e-30 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 221
Acc65I GGTACC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 325
AccI GTMKAC 1 cut(s) 126
AclWI GGATC 1 cut(s) 543
AcoI YGGCCR 1 cut(s) 427
AcsI RAATTY 2 cut(s) 5, 115
AfaI GTAC 1 cut(s) 139
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 6 cut(s) 10, 23, 211, 251, 542, 575
AjuI GAANNNNNNNTTGG 2 cut(s) 545, 577
AluBI AGCT 1 cut(s) 594
AluI AGCT 1 cut(s) 594
Alw26I GTCTC 2 cut(s) 47, 501
AlwI GGATC 1 cut(s) 543
AoxI GGCC 3 cut(s) 92, 427, 579
ApeKI GCWGC 2 cut(s) 269, 482
ApoI RAATTY 2 cut(s) 5, 115
Asp718I GGTACC 1 cut(s) 137
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 2 cut(s) 302, 489
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 2 cut(s) 302, 489
BaeI ACNNNNGTAYC 2 cut(s) 129, 162
BalI TGGCCA 1 cut(s) 429
BanI GGYRCC 1 cut(s) 137
BbvI GCAGC 2 cut(s) 256, 494
BccI CCATC 2 cut(s) 116, 293
BcoDI GTCTC 2 cut(s) 47, 501
BfaI CTAG 4 cut(s) 99, 341, 591, 595
BisI GCNGC 2 cut(s) 270, 483
BlsI GCNGC 2 cut(s) 271, 484
Bme18I GGWCC 2 cut(s) 302, 489
BmgT120I GGNCC 2 cut(s) 302, 489
BmiI GGNNCC 1 cut(s) 139
BmsI GCATC 2 cut(s) 53, 432
BpuEI CTTGAG 3 cut(s) 23, 137, 494
BsaI GGTCTC 2 cut(s) 47, 501
BsaJI CCNNGG 1 cut(s) 430
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 582
Bse3DI GCAATG 2 cut(s) 247, 336
BseDI CCNNGG 1 cut(s) 430
BseGI GGATG 1 cut(s) 68
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMI GCAATG 2 cut(s) 247, 336
BseNI ACTGG 1 cut(s) 582
BseXI GCAGC 2 cut(s) 256, 494
BsgI GTGCAG 1 cut(s) 501
BshFI GGCC 3 cut(s) 94, 429, 581
BshNI GGYRCC 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 325
BsmAI GTCTC 2 cut(s) 47, 501
BsnI GGCC 3 cut(s) 94, 429, 581
Bso31I GGTCTC 2 cut(s) 47, 501
Bsp143I GATC 2 cut(s) 69, 535
BspANI GGCC 3 cut(s) 94, 429, 581
BspLI GGNNCC 1 cut(s) 139
BspPI GGATC 1 cut(s) 543
BspT107I GGYRCC 1 cut(s) 137
BspTNI GGTCTC 2 cut(s) 47, 501
BsrDI GCAATG 2 cut(s) 247, 336
BsrI ACTGG 1 cut(s) 582
BssECI CCNNGG 1 cut(s) 430
BssMI GATC 2 cut(s) 69, 535
BssT1I CCWWGG 1 cut(s) 430
Bst4CI ACNGT 2 cut(s) 145, 393
Bst6I CTCTTC 1 cut(s) 589
BstF5I GGATG 1 cut(s) 68
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 2 cut(s) 72, 538
BstMAI GTCTC 2 cut(s) 47, 501
BstMBI GATC 2 cut(s) 69, 535
BstV1I GCAGC 2 cut(s) 256, 494
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BsuRI GGCC 3 cut(s) 94, 429, 581
BtgZI GCGATG 2 cut(s) 93, 455
BtsCI GGATG 1 cut(s) 68
BtsIMutI CAGTG 1 cut(s) 141
CfoI GCGC 1 cut(s) 240
Cfr13I GGNCC 2 cut(s) 302, 489
Csp6I GTAC 1 cut(s) 138
CviAII CATG 6 cut(s) 48, 73, 121, 188, 352, 384
CviJI RGCY 6 cut(s) 94, 318, 429, 560, 581, 594
CviKI_1 RGCY 6 cut(s) 94, 318, 429, 560, 581, 594
CviQI GTAC 1 cut(s) 138
DpnI GATC 2 cut(s) 71, 537
DpnII GATC 2 cut(s) 69, 535
EaeI YGGCCR 1 cut(s) 427
Eam1104I CTCTTC 1 cut(s) 589
EarI CTCTTC 1 cut(s) 589
Eco130I CCWWGG 1 cut(s) 430
Eco31I GGTCTC 2 cut(s) 47, 501
Eco47I GGWCC 2 cut(s) 302, 489
EcoRI GAATTC 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
FaeI CATG 6 cut(s) 51, 76, 124, 191, 355, 387
FatI CATG 6 cut(s) 47, 72, 120, 187, 351, 383
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 2 cut(s) 270, 483
FokI GGATG 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 270, 483
FspBI CTAG 4 cut(s) 99, 341, 591, 595
GlaI GCGC 1 cut(s) 239
GluI GCNGC 2 cut(s) 270, 483
HaeIII GGCC 3 cut(s) 94, 429, 581
HhaI GCGC 1 cut(s) 240
Hin1II CATG 6 cut(s) 51, 76, 124, 191, 355, 387
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HincII GTYRAC 2 cut(s) 31, 283
HindII GTYRAC 2 cut(s) 31, 283
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 3 cut(s) 31, 127, 283
Hpy188I TCNGA 1 cut(s) 397
Hpy188III TCNNGA 2 cut(s) 473, 539
Hpy8I GTNNAC 3 cut(s) 31, 127, 283
HpyAV CCTTC 3 cut(s) 205, 285, 392
HpyCH4III ACNGT 2 cut(s) 145, 393
HpyCH4V TGCA 3 cut(s) 66, 367, 482
Hsp92II CATG 6 cut(s) 51, 76, 124, 191, 355, 387
HspAI GCGC 1 cut(s) 238
KpnI GGTACC 1 cut(s) 141
Kzo9I GATC 2 cut(s) 69, 535
LpnPI CCDG 4 cut(s) 156, 273, 311, 563
Lsp1109I GCAGC 2 cut(s) 256, 494
LweI GCATC 2 cut(s) 53, 432
MaeI CTAG 4 cut(s) 99, 341, 591, 595
MaeIII GTNAC 2 cut(s) 256, 387
MalI GATC 2 cut(s) 71, 537
MboI GATC 2 cut(s) 69, 535
MboII GAAGA 3 cut(s) 190, 399, 576
MflI RGATCY 1 cut(s) 535
MlsI TGGCCA 1 cut(s) 429
MluCI AATT 4 cut(s) 5, 115, 203, 274
MluNI TGGCCA 1 cut(s) 429
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 5 cut(s) 220, 469, 515, 521, 592
Mox20I TGGCCA 1 cut(s) 429
MscI TGGCCA 1 cut(s) 429
MslI CAYNNNNRTG 1 cut(s) 71
Msp20I TGGCCA 1 cut(s) 429
NdeII GATC 2 cut(s) 69, 535
NlaIII CATG 6 cut(s) 51, 76, 124, 191, 355, 387
NlaIV GGNNCC 1 cut(s) 139
NmuCI GTSAC 2 cut(s) 256, 387
PflMI CCANNNNNTGG 1 cut(s) 325
PkrI GCNGC 2 cut(s) 271, 484
PsiI TTATAA 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 139
PspPI GGNCC 2 cut(s) 302, 489
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 1 cut(s) 139
RsaNI GTAC 1 cut(s) 138
RseI CAYNNNNRTG 1 cut(s) 71
SatI GCNGC 2 cut(s) 270, 483
Sau3AI GATC 2 cut(s) 69, 535
Sau96I GGNCC 2 cut(s) 302, 489
SetI ASST 6 cut(s) 59, 139, 231, 436, 513, 596
SfaNI GCATC 2 cut(s) 53, 432
SinI GGWCC 2 cut(s) 302, 489
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 3 cut(s) 38, 152, 473
SmoI CTYRAG 3 cut(s) 38, 152, 473
Sse9I AATT 4 cut(s) 5, 115, 203, 274
SspMI CTAG 4 cut(s) 99, 341, 591, 595
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 2 cut(s) 145, 393
TaqI TCGA 1 cut(s) 513
TasI AATT 4 cut(s) 5, 115, 203, 274
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 2 cut(s) 256, 387
TseI GCWGC 2 cut(s) 269, 482
Tsp45I GTSAC 2 cut(s) 256, 387
TspDTI ATGAA 3 cut(s) 96, 109, 576
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 325
VpaK11BI GGWCC 2 cut(s) 302, 489
XapI RAATTY 2 cut(s) 5, 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XmiI GTMKAC 1 cut(s) 126
XspI CTAG 4 cut(s) 99, 341, 591, 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.