Rw2G043790

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000220
Physical Location & Seq
Forward (+)
70333573 .. 70345348
11776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043790.1

Sequence Viewer

Length: 1668 bp
ATGGCAAGCAGCGGTAGAGGTAGAAATGATCCAGCATGGAATGGAGGAATTACAAGATTGAAGAGTCATTTATCGGGTTATGATCCTCACAAGAGTGTAAAGAAATGTGATAAGGTGCCATCGGAGATCAAAACAGAGGTGATAGCATGGATAAAGAGAAAAGATTCTATTAAGCAAGAAAAGGCAGCTGTTCAAGATGATATTAGAGAAGAACTCCGCAATGATTATCTTGGCAATCAAACAAGTCTGTACAATGACGATGACGACAATGATGATGATGATGATGGATTTCAGTATCCACCAGACATGCACCCTGCAGAACAACAAGATTATCGTGCTGCATTAAGACTATCAACCGTAGATCGTGATAAGAACTCTCATTATGTGGATACTCACAAAGAAACCTGGAAAATATATGGATGCACTATCATGTGTGATGGATGGACTGGCCTAACCAAAATGTCTATTCTAAATTTCACAGTGTATTCAAAAGGTTCAACTGTGTTTTTGAAGTCTGTAGATGCTTCACATGTGAAAAAAAATGCTCAGTACATAGAACTATTGTTAGATGAGATTATCCAAGATGTTGGACCAGAAAATGTTGTCCAGATTGTCACTGACAATGCATCAGCATTTAAAAAGGCCGGAAGGGAACTACAGAAAAAATATCCACTGTTCTGGATCCCTGTAACTAACTACATCTACAATCATGGTTGGGTACTGGCTGAAACGAGGAGCATCAAGAAGGGAGATTTGATTCGACAGGGTAAAACTCGATTTGCGACAAATCACATTGCCATCGACAGTATTTTGAAGAAGAAATCTAATTTGAGAAAATTGTTTACAAGTAGCACATGGAATGAGAATGCAGCGAGCAAGACCAGAGATGGCAAAAGGATAGAAAAAAGAGTCCTCGATGGGACATTTTGGGATGGCATGGAAGCAGTCCATAAAATTTATAAGCCATTGTATGAGATTCTTCGAATTGTTGACACAAAAATTCAGCCAACCATGCCCATATTACATGATATGTTTGAAAGAGTGAAACAACAAATATCACAGATGAGAGGAAAGAAATGGGTGCTTAAAATCATCAATGATCGATGGGATAACACATTAAGTCAACCATTGCATGTAGCAGATATTATTTCTCTATCTCGAAATACTTTTGGTTTCTTGTTTGTAGCACATTTCTTAAACCCAAATTTTCATTATCGACATGATGTTGGTTATAGCCCACGTTTCACAAACTCTCTGGCAACAGTTGTTGAACAATTTAACATAAATGGAGAAATTGCAGATACATTTGTGGATGAGAGATGCAAAAGAATCATTTGGGTCAACCATAGCAAGAAAAACAAGAGAAACCAGAAATACAGAGTCTTATCTGAAGAAGTTGTTAAAAACCCAAAAGATAATTCAGATAGTGATGATGCGTGGGGTAGTACAGCAACTCCAGATAGTTCTGATGATGGTGGAGAGGGTGGAAGCGAAACAGGTGGTGGAGGTGAAAGATATGAATATGGACAATCTTCTAGCGCAATGGGATCTCAAATGAATCTCATGAAGGCAACAATCAATTTGGTTATGATGCTTATGGATATAATCAATATGGAGAAGTATAAGATCAATCATCTAGTTGGGTTCATCCTTATTATCCCCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

555

Amino Acids

63.49

Weight (kDa)

8.5

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 126 - 232 4e-34 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 960
AccB1I GGYRCC 1 cut(s) 115
AciI CCGC 2 cut(s) 12, 217
AclWI GGATC 5 cut(s) 23, 77, 676, 689, 1555
AcsI RAATTY 4 cut(s) 472, 954, 999, 1204
AcuI CTGAAG 1 cut(s) 1410
AfaI GTAC 4 cut(s) 251, 551, 720, 1447
AflIII ACRYGT 1 cut(s) 529
AgsI TTSAA 8 cut(s) 61, 194, 489, 498, 511, 814, 1037, 1271
AjnI CCWGG 1 cut(s) 404
AjuI GAANNNNNNNTTGG 2 cut(s) 1402, 1434
AleI CACNNNNGTG 1 cut(s) 93
AluBI AGCT 1 cut(s) 188
AluI AGCT 1 cut(s) 188
AlwI GGATC 5 cut(s) 23, 77, 676, 689, 1555
AoxI GGCC 2 cut(s) 448, 642
ApeKI GCWGC 4 cut(s) 9, 185, 338, 869
ApoI RAATTY 4 cut(s) 472, 954, 999, 1204
Asp700I GAANNNNTTC 1 cut(s) 163
AspLEI GCGC 1 cut(s) 1541
AspS9I GGNCC 1 cut(s) 590
AsuHPI GGTGA 2 cut(s) 151, 1520
AsuII TTCGAA 1 cut(s) 982
AvaII GGWCC 1 cut(s) 590
BaeI ACNNNNGTAYC 2 cut(s) 1293, 1326
BamHI GGATCC 1 cut(s) 681
BanI GGYRCC 1 cut(s) 115
BbvI GCAGC 4 cut(s) 21, 197, 325, 881
BciT130I CCWGG 1 cut(s) 406
BciVI GTATCC 2 cut(s) 306, 382
BfaI CTAG 2 cut(s) 1536, 1637
BfmI CTRYAG 3 cut(s) 315, 516, 656
BfuI GTATCC 2 cut(s) 306, 382
BisI GCNGC 4 cut(s) 10, 186, 339, 870
BlsI GCNGC 4 cut(s) 11, 187, 340, 871
Bme1390I CCNGG 1 cut(s) 406
Bme18I GGWCC 1 cut(s) 590
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 2 cut(s) 117, 683
BmrFI CCNGG 1 cut(s) 406
BmsI GCATC 7 cut(s) 410, 511, 635, 747, 1310, 1423, 1581
BplI GAGNNNNNCTC 2 cut(s) 198, 230
BpmI CTGGAG 1 cut(s) 1440
Bpu14I TTCGAA 1 cut(s) 982
Bsa29I ATCGAT 1 cut(s) 1102
BsaXI ACNNNNNCTCC 2 cut(s) 1438, 1468
Bse1I ACTGG 2 cut(s) 451, 726
Bse3DI GCAATG 4 cut(s) 226, 792, 1127, 1548
BseBI CCWGG 1 cut(s) 406
BseCI ATCGAT 1 cut(s) 1102
BseGI GGATG 5 cut(s) 425, 446, 937, 1318, 1647
BseMI GCAATG 4 cut(s) 226, 792, 1127, 1548
BseMII CTCAG 1 cut(s) 560
BseNI ACTGG 2 cut(s) 451, 726
BseRI GAGGAG 1 cut(s) 748
BseXI GCAGC 4 cut(s) 21, 197, 325, 881
BshFI GGCC 2 cut(s) 450, 644
BshNI GGYRCC 1 cut(s) 115
BshVI ATCGAT 1 cut(s) 1102
BsiSI CCGG 1 cut(s) 645
BslFI GGGAC 1 cut(s) 934
BsmFI GGGAC 1 cut(s) 934
BsmI GAATGC 1 cut(s) 871
BsnI GGCC 2 cut(s) 450, 644
Bsp119I TTCGAA 1 cut(s) 982
Bsp1407I TGTACA 1 cut(s) 249
Bsp143I GATC 8 cut(s) 28, 82, 126, 361, 681, 1099, 1547, 1626
BspACI CCGC 2 cut(s) 12, 217
BspANI GGCC 2 cut(s) 450, 644
BspCNI CTCAG 1 cut(s) 559
BspDI ATCGAT 1 cut(s) 1102
BspHI TCATGA 1 cut(s) 1563
BspLI GGNNCC 2 cut(s) 117, 683
BspMAI CTGCAG 1 cut(s) 319
BspPI GGATC 5 cut(s) 23, 77, 676, 689, 1555
BspT104I TTCGAA 1 cut(s) 982
BspT107I GGYRCC 1 cut(s) 115
BsrDI GCAATG 4 cut(s) 226, 792, 1127, 1548
BsrGI TGTACA 1 cut(s) 249
BsrI ACTGG 2 cut(s) 451, 726
BssMI GATC 8 cut(s) 28, 82, 126, 361, 681, 1099, 1547, 1626
Bst2UI CCWGG 1 cut(s) 406
Bst4CI ACNGT 6 cut(s) 358, 481, 502, 675, 806, 1264
Bst6I CTCTTC 1 cut(s) 56
BstAUI TGTACA 1 cut(s) 249
BstBI TTCGAA 1 cut(s) 982
BstC8I GCNNGC 2 cut(s) 7, 874
BstDEI CTNAG 1 cut(s) 546
BstF5I GGATG 5 cut(s) 425, 446, 937, 1318, 1647
BstHHI GCGC 1 cut(s) 1541
BstKTI GATC 8 cut(s) 31, 85, 129, 364, 684, 1102, 1550, 1629
BstMBI GATC 8 cut(s) 28, 82, 126, 361, 681, 1099, 1547, 1626
BstMWI GCNNNNNNNGC 1 cut(s) 1012
BstNI CCWGG 1 cut(s) 406
BstNSI RCATGY 3 cut(s) 310, 533, 1136
BstSCI CCNGG 1 cut(s) 404
BstSFI CTRYAG 3 cut(s) 315, 516, 656
BstV1I GCAGC 4 cut(s) 21, 197, 325, 881
BstX2I RGATCY 2 cut(s) 681, 1547
BstXI CCANNNNNNTGG 2 cut(s) 587, 678
BstYI RGATCY 2 cut(s) 681, 1547
Bsu15I ATCGAT 1 cut(s) 1102
BsuI GTATCC 2 cut(s) 306, 382
BsuRI GGCC 2 cut(s) 450, 644
BsuTUI ATCGAT 1 cut(s) 1102
BtsCI GGATG 5 cut(s) 425, 446, 937, 1318, 1647
BtsIMutI CAGTG 3 cut(s) 486, 615, 671
Cac8I GCNNGC 2 cut(s) 7, 874
CciI TCATGA 1 cut(s) 1563
CfoI GCGC 1 cut(s) 1541
Cfr13I GGNCC 1 cut(s) 590
ClaI ATCGAT 1 cut(s) 1102
Csp6I GTAC 4 cut(s) 250, 550, 719, 1446
CviJI RGCY 7 cut(s) 188, 450, 644, 725, 964, 1006, 1236
CviKI_1 RGCY 7 cut(s) 188, 450, 644, 725, 964, 1006, 1236
CviQI GTAC 4 cut(s) 250, 550, 719, 1446
DdeI CTNAG 1 cut(s) 546
DpnI GATC 8 cut(s) 30, 84, 128, 363, 683, 1101, 1549, 1628
DpnII GATC 8 cut(s) 28, 82, 126, 361, 681, 1099, 1547, 1626
DraI TTTAAA 1 cut(s) 637
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 1410
EcoRII CCWGG 1 cut(s) 404
EcoT22I ATGCAT 1 cut(s) 628
FaqI GGGAC 1 cut(s) 934
Fnu4HI GCNGC 4 cut(s) 10, 186, 339, 870
FokI GGATG 5 cut(s) 432, 453, 944, 1325, 1634
Fsp4HI GCNGC 4 cut(s) 10, 186, 339, 870
FspBI CTAG 2 cut(s) 1536, 1637
GlaI GCGC 1 cut(s) 1540
GluI GCNGC 4 cut(s) 10, 186, 339, 870
GsuI CTGGAG 1 cut(s) 1440
HaeIII GGCC 2 cut(s) 450, 644
HapII CCGG 1 cut(s) 645
HhaI GCGC 1 cut(s) 1541
Hin6I GCGC 1 cut(s) 1539
HinP1I GCGC 1 cut(s) 1539
HincII GTYRAC 3 cut(s) 991, 1124, 1342
HindII GTYRAC 3 cut(s) 991, 1124, 1342
HinfI GANTC 8 cut(s) 64, 164, 757, 909, 976, 1329, 1380, 1558
HpaII CCGG 1 cut(s) 645
HphI GGTGA 2 cut(s) 151, 1520
Hpy166II GTNNAC 4 cut(s) 843, 991, 1124, 1342
Hpy188I TCNGA 4 cut(s) 124, 1390, 1423, 1468
Hpy188III TCNNGA 8 cut(s) 194, 365, 607, 679, 742, 1158, 1457, 1564
Hpy8I GTNNAC 4 cut(s) 843, 991, 1124, 1342
HpyAV CCTTC 3 cut(s) 642, 739, 1561
HpyCH4III ACNGT 6 cut(s) 358, 481, 502, 675, 806, 1264
HpyCH4IV ACGT 1 cut(s) 1240
HpyCH4V TGCA 9 cut(s) 310, 317, 341, 423, 626, 869, 1132, 1298, 1323
HpyF10VI GCNNNNNNNGC 1 cut(s) 1012
HpyF3I CTNAG 1 cut(s) 546
HpySE526I ACGT 1 cut(s) 1240
HspAI GCGC 1 cut(s) 1539
Kzo9I GATC 8 cut(s) 28, 82, 126, 361, 681, 1099, 1547, 1626
LmnI GCTCC 1 cut(s) 735
Lsp1109I GCAGC 4 cut(s) 21, 197, 325, 881
LweI GCATC 7 cut(s) 410, 511, 635, 747, 1310, 1423, 1581
MaeI CTAG 2 cut(s) 1536, 1637
MaeII ACGT 1 cut(s) 1240
MaeIII GTNAC 2 cut(s) 613, 688
MalI GATC 8 cut(s) 30, 84, 128, 363, 683, 1101, 1549, 1628
MboI GATC 8 cut(s) 28, 82, 126, 361, 681, 1099, 1547, 1626
MboII GAAGA 7 cut(s) 73, 221, 826, 829, 971, 1403, 1524
MflI RGATCY 2 cut(s) 681, 1547
MlyI GAGTC 3 cut(s) 73, 918, 1389
MmeI TCCRAC 1 cut(s) 568
MnlI CCTC 9 cut(s) 11, 38, 96, 130, 726, 923, 1061, 1474, 1499
Mph1103I ATGCAT 1 cut(s) 628
MroXI GAANNNNTTC 1 cut(s) 163
MseI TTAA 8 cut(s) 171, 344, 636, 1086, 1118, 1196, 1278, 1401
MslI CAYNNNNRTG 2 cut(s) 93, 428
MspA1I CMGCKG 2 cut(s) 12, 188
MspI CCGG 1 cut(s) 645
MspR9I CCNGG 1 cut(s) 406
Mva1269I GAATGC 1 cut(s) 871
MvaI CCWGG 1 cut(s) 406
MwoI GCNNNNNNNGC 1 cut(s) 1012
NdeII GATC 8 cut(s) 28, 82, 126, 361, 681, 1099, 1547, 1626
NlaIV GGNNCC 2 cut(s) 117, 683
NmuCI GTSAC 1 cut(s) 613
NsiI ATGCAT 1 cut(s) 628
NspI RCATGY 3 cut(s) 310, 533, 1136
NspV TTCGAA 1 cut(s) 982
OliI CACNNNNGTG 1 cut(s) 93
PagI TCATGA 1 cut(s) 1563
PciI ACATGT 1 cut(s) 529
PctI GAATGC 1 cut(s) 871
PdmI GAANNNNTTC 1 cut(s) 163
PfeI GAWTC 5 cut(s) 164, 757, 976, 1329, 1558
PkrI GCNGC 4 cut(s) 11, 187, 340, 871
PleI GAGTC 3 cut(s) 72, 917, 1388
PpsI GAGTC 3 cut(s) 72, 917, 1388
PscI ACATGT 1 cut(s) 529
PsiI TTATAA 1 cut(s) 960
Psp6I CCWGG 1 cut(s) 404
PspGI CCWGG 1 cut(s) 404
PspN4I GGNNCC 2 cut(s) 117, 683
PspPI GGNCC 1 cut(s) 590
PstI CTGCAG 1 cut(s) 319
PsuI RGATCY 2 cut(s) 681, 1547
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 4 cut(s) 251, 551, 720, 1447
RsaNI GTAC 4 cut(s) 250, 550, 719, 1446
RseI CAYNNNNRTG 2 cut(s) 93, 428
SaqAI TTAA 8 cut(s) 171, 344, 636, 1086, 1118, 1196, 1278, 1401
SatI GCNGC 4 cut(s) 10, 186, 339, 870
Sau3AI GATC 8 cut(s) 28, 82, 126, 361, 681, 1099, 1547, 1626
Sau96I GGNCC 1 cut(s) 590
SchI GAGTC 3 cut(s) 73, 918, 1389
ScrFI CCNGG 1 cut(s) 406
SetI ASST 9 cut(s) 22, 117, 141, 190, 407, 496, 1243, 1501, 1510
SfaNI GCATC 7 cut(s) 410, 511, 635, 747, 1310, 1423, 1581
SfcI CTRYAG 3 cut(s) 315, 516, 656
SfuI TTCGAA 1 cut(s) 982
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 2 cut(s) 93, 428
SsiI CCGC 2 cut(s) 12, 217
SspMI CTAG 2 cut(s) 1536, 1637
StyD4I CCNGG 1 cut(s) 404
TaaI ACNGT 6 cut(s) 358, 481, 502, 675, 806, 1264
TaiI ACGT 1 cut(s) 1243
TaqI TCGA 8 cut(s) 760, 775, 801, 915, 982, 1102, 1159, 1216
TatI WGTACW 3 cut(s) 249, 549, 1445
TfiI GAWTC 5 cut(s) 164, 757, 976, 1329, 1558
Tru1I TTAA 8 cut(s) 171, 344, 636, 1086, 1118, 1196, 1278, 1401
Tru9I TTAA 8 cut(s) 171, 344, 636, 1086, 1118, 1196, 1278, 1401
TscAI CASTG 3 cut(s) 486, 622, 678
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 4 cut(s) 9, 185, 338, 869
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 5 cut(s) 1199, 1533, 1571, 1580, 1636
TspRI CASTG 3 cut(s) 486, 622, 678
VpaK11BI GGWCC 1 cut(s) 590
XapI RAATTY 4 cut(s) 472, 954, 999, 1204
XceI RCATGY 3 cut(s) 310, 533, 1136
XmnI GAANNNNTTC 1 cut(s) 163
XspI CTAG 2 cut(s) 1536, 1637
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.