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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 149 |
| AccB1I | GGYRCC | 1 cut(s) | 149 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 209 |
| AccI | GTMKAC | 1 cut(s) | 177 |
| AclWI | GGATC | 1 cut(s) | 323 |
| AcoI | YGGCCR | 1 cut(s) | 211 |
| AfaI | GTAC | 2 cut(s) | 151, 168 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 137, 209 |
| AflIII | ACRYGT | 1 cut(s) | 160 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 26, 58 |
| AluBI | AGCT | 1 cut(s) | 229 |
| AluI | AGCT | 1 cut(s) | 229 |
| Alw26I | GTCTC | 1 cut(s) | 303 |
| AlwI | GGATC | 1 cut(s) | 323 |
| AoxI | GGCC | 1 cut(s) | 211 |
| ApeKI | GCWGC | 1 cut(s) | 124 |
| Asp718I | GGTACC | 1 cut(s) | 149 |
| BalI | TGGCCA | 1 cut(s) | 213 |
| BanI | GGYRCC | 1 cut(s) | 149 |
| BbvI | GCAGC | 1 cut(s) | 136 |
| BccI | CCATC | 2 cut(s) | 86, 140 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 103, 137 |
| BcoDI | GTCTC | 1 cut(s) | 303 |
| BfaI | CTAG | 1 cut(s) | 132 |
| BisI | GCNGC | 1 cut(s) | 125 |
| BlsI | GCNGC | 1 cut(s) | 126 |
| BmiI | GGNNCC | 1 cut(s) | 151 |
| BmsI | GCATC | 1 cut(s) | 235 |
| Bsa29I | ATCGAT | 1 cut(s) | 318 |
| BsaAI | YACGTR | 1 cut(s) | 161 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 137, 209 |
| BseCI | ATCGAT | 1 cut(s) | 318 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 137, 209 |
| BseMII | CTCAG | 1 cut(s) | 297 |
| BseXI | GCAGC | 1 cut(s) | 136 |
| BshFI | GGCC | 1 cut(s) | 213 |
| BshNI | GGYRCC | 1 cut(s) | 149 |
| BshVI | ATCGAT | 1 cut(s) | 318 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 137, 209 |
| BsmAI | GTCTC | 1 cut(s) | 303 |
| BsnI | GGCC | 1 cut(s) | 213 |
| Bsp1407I | TGTACA | 1 cut(s) | 166 |
| Bsp143I | GATC | 1 cut(s) | 315 |
| BspANI | GGCC | 1 cut(s) | 213 |
| BspCNI | CTCAG | 1 cut(s) | 298 |
| BspDI | ATCGAT | 1 cut(s) | 318 |
| BspLI | GGNNCC | 1 cut(s) | 151 |
| BspPI | GGATC | 1 cut(s) | 323 |
| BspT107I | GGYRCC | 1 cut(s) | 149 |
| BsrGI | TGTACA | 1 cut(s) | 166 |
| BssMI | GATC | 1 cut(s) | 315 |
| BssNAI | GTATAC | 1 cut(s) | 178 |
| Bst1107I | GTATAC | 1 cut(s) | 178 |
| Bst4CI | ACNGT | 1 cut(s) | 208 |
| BstAUI | TGTACA | 1 cut(s) | 166 |
| BstBAI | YACGTR | 1 cut(s) | 161 |
| BstC8I | GCNNGC | 1 cut(s) | 122 |
| BstDEI | CTNAG | 1 cut(s) | 306 |
| BstKTI | GATC | 1 cut(s) | 318 |
| BstMAI | GTCTC | 1 cut(s) | 303 |
| BstMBI | GATC | 1 cut(s) | 315 |
| BstNSI | RCATGY | 1 cut(s) | 124 |
| BstV1I | GCAGC | 1 cut(s) | 136 |
| BstZ17I | GTATAC | 1 cut(s) | 178 |
| Bsu15I | ATCGAT | 1 cut(s) | 318 |
| BsuRI | GGCC | 1 cut(s) | 213 |
| BsuTUI | ATCGAT | 1 cut(s) | 318 |
| Cac8I | GCNNGC | 1 cut(s) | 122 |
| ClaI | ATCGAT | 1 cut(s) | 318 |
| Csp6I | GTAC | 2 cut(s) | 150, 167 |
| CviAII | CATG | 3 cut(s) | 56, 121, 234 |
| CviJI | RGCY | 2 cut(s) | 213, 229 |
| CviKI_1 | RGCY | 2 cut(s) | 213, 229 |
| CviQI | GTAC | 2 cut(s) | 150, 167 |
| DdeI | CTNAG | 1 cut(s) | 306 |
| DpnI | GATC | 1 cut(s) | 317 |
| DpnII | GATC | 1 cut(s) | 315 |
| DraI | TTTAAA | 1 cut(s) | 241 |
| EaeI | YGGCCR | 1 cut(s) | 211 |
| FaeI | CATG | 3 cut(s) | 59, 124, 237 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 241, 273 |
| FatI | CATG | 3 cut(s) | 55, 120, 233 |
| FblI | GTMKAC | 1 cut(s) | 177 |
| Fnu4HI | GCNGC | 1 cut(s) | 125 |
| Fsp4HI | GCNGC | 1 cut(s) | 125 |
| FspBI | CTAG | 1 cut(s) | 132 |
| GluI | GCNGC | 1 cut(s) | 125 |
| HaeIII | GGCC | 1 cut(s) | 213 |
| Hin1II | CATG | 3 cut(s) | 59, 124, 237 |
| Hpy166II | GTNNAC | 1 cut(s) | 178 |
| Hpy8I | GTNNAC | 1 cut(s) | 178 |
| HpyCH4III | ACNGT | 1 cut(s) | 208 |
| HpyCH4IV | ACGT | 1 cut(s) | 160 |
| HpyCH4V | TGCA | 5 cut(s) | 62, 120, 124, 197, 248 |
| HpyF3I | CTNAG | 1 cut(s) | 306 |
| HpySE526I | ACGT | 1 cut(s) | 160 |
| Hsp92II | CATG | 3 cut(s) | 59, 124, 237 |
| KpnI | GGTACC | 1 cut(s) | 153 |
| Kzo9I | GATC | 1 cut(s) | 315 |
| Lsp1109I | GCAGC | 1 cut(s) | 136 |
| LweI | GCATC | 1 cut(s) | 235 |
| MaeI | CTAG | 1 cut(s) | 132 |
| MaeII | ACGT | 1 cut(s) | 160 |
| MalI | GATC | 1 cut(s) | 317 |
| MboI | GATC | 1 cut(s) | 315 |
| MboII | GAAGA | 1 cut(s) | 264 |
| MlsI | TGGCCA | 1 cut(s) | 213 |
| MluCI | AATT | 3 cut(s) | 80, 185, 215 |
| MluNI | TGGCCA | 1 cut(s) | 213 |
| MmeI | TCCRAC | 1 cut(s) | 118 |
| MnlI | CCTC | 1 cut(s) | 31 |
| Mox20I | TGGCCA | 1 cut(s) | 213 |
| MscI | TGGCCA | 1 cut(s) | 213 |
| MseI | TTAA | 1 cut(s) | 240 |
| Msp20I | TGGCCA | 1 cut(s) | 213 |
| NdeII | GATC | 1 cut(s) | 315 |
| NlaIII | CATG | 3 cut(s) | 59, 124, 237 |
| NlaIV | GGNNCC | 1 cut(s) | 151 |
| NspI | RCATGY | 1 cut(s) | 124 |
| PaeI | GCATGC | 1 cut(s) | 124 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 209 |
| PkrI | GCNGC | 1 cut(s) | 126 |
| Ppu21I | YACGTR | 1 cut(s) | 161 |
| PspN4I | GGNNCC | 1 cut(s) | 151 |
| RsaI | GTAC | 2 cut(s) | 151, 168 |
| RsaNI | GTAC | 2 cut(s) | 150, 167 |
| SaqAI | TTAA | 1 cut(s) | 240 |
| SatI | GCNGC | 1 cut(s) | 125 |
| Sau3AI | GATC | 1 cut(s) | 315 |
| SetI | ASST | 5 cut(s) | 68, 133, 163, 195, 231 |
| SfaNI | GCATC | 1 cut(s) | 235 |
| SphI | GCATGC | 1 cut(s) | 124 |
| Sse9I | AATT | 3 cut(s) | 80, 185, 215 |
| SspMI | CTAG | 1 cut(s) | 132 |
| TaaI | ACNGT | 1 cut(s) | 208 |
| TaiI | ACGT | 1 cut(s) | 163 |
| TaqI | TCGA | 2 cut(s) | 286, 318 |
| TaqII | GACCGA | 1 cut(s) | 104 |
| TasI | AATT | 3 cut(s) | 80, 185, 215 |
| TatI | WGTACW | 1 cut(s) | 166 |
| Tru1I | TTAA | 1 cut(s) | 240 |
| Tru9I | TTAA | 1 cut(s) | 240 |
| TseI | GCWGC | 1 cut(s) | 124 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 209 |
| XceI | RCATGY | 1 cut(s) | 124 |
| XmiI | GTMKAC | 1 cut(s) | 177 |
| XspI | CTAG | 1 cut(s) | 132 |