MD13G1044700.v1.1

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000220
Physical Location & Seq
Forward (+)
3100342 .. 3105261
4920 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1044700.v1.1.491

Sequence Viewer

Length: 2319 bp
ATGGTAGTGGGATGTCAACAGGCCGGTGTTGGAGTACAACCTCCCACTCCCTATGAGATAAGAAACAAATATTTGGATATGGAGTATAAAGACATTGGCGAGTATGTTAACAAGTTGAGGTCAAAGTGGGAAACTAATGGTTGCACAATCATGTGTGACGACGGATGGACCGGCCCGACCAGATTATCTATCATCAACTTCATGGTATACTCCAAGGGCAAGACAATTTTTCTGAAGTCTGTTGATGCTTCAGACCATATAAAGAATTACAAGTATATTTACAAATTATTGAGGGATGTAATCATGGAGGTGGGAGAGCATAATGTTGTCCAAGTCGTGACCGACAACGGTTCTGCATTTGTCAAAGCTGGAAAAAAGTTAATGAAGCATCATAATGTGTTTTGGACATCATGTGCAGCACATTGTATTGATCTTATGTTTGAGGCAATGGGAAAGAGAGAGAATGTTGCTACTGTGGTCAAAAGAGCTAGAACGATCACTAATTATATTTACAATCACGGTTGGTTGTTGGCAAAGATGCGTGAATTTTGCAGAGGAGAAATTATTCGTCCAGCTACCACTCGATTCGCCACCAACTATATTGCATTAAACAGCCTACTCAAGAAGAAAGCAGGGTTGAAGCAACTATTCACTAGTGACGATTGGGCCAACCACAATTTCAGCCGCTCAAATACAGGTCGTATGGTGGAAAGTATAGTGCTTGATCATGCTTTTTGGAGTCAAACAGAACATGTGTGTCAAGTGTTTGAACCTCTTTACAAAGTTTTACGGATCGTTGACACAGAAGTGTATCCTACTATGGGGGCTGTATATGAATTGATGCGTGTAGTGAAGGATGAATTGGAAAGAAAACATGGTGCAAGGTGGGTCGTAAAAATAATTGAAGACCGATGGTATAAAACATTATACCACGATTTGCATGCAGCAGCATATTATTTGAATCCCCGATACCAATACAGACCCGGTGTTGGAGATGATGGTAACCTTATACGTGCTGTACATAATGTATACAATAAATTAGACCCTGCATCACCAGCAGTTGGCCAATTTGGAAATGAGCTAACATGGTTTAAAGATGCAAGAAGAACATTTGGAGAACCAACATCAGTTGCTGCTCGAACAAATATGTCTCCTACTGAATGGTGGATCATGTATGGGACCGATGCACCAACTGTGAGAAAGTTAGCAATAAAAGTATTATCACAAACGGCTTCCTCATCTGCTTGTGAAAGAAATTGGAGCACATTTGCACTCATCCACACAAAGCAAAGAAATAAGTTGGCTCATAGTAGCTTGGAAAAATTAGTTTATTGCTACTACAACATGAAGCTTCAAATTCGAGATAAGGAAGCAGAAATCGATCATGTCGACCGTGGTGACCCACTAGATGTGTTTGATATTGTTGGTGAAGATGATGATACGGAGGGTAACCAACTTTTTCAATGGATTAGACCTCTTCATTTAGATGATGATGATGAAGGCAACCCAGCTCCCAGAGTTGCTGAAGAAGCACGTAATGAAGGGATAAATGTAGAAAGAGTATTAGAGGAGGAGGTGGGATCTAGCAGCACTGACTCTTTCGAAGAACTTTTGCAGCCAAGACCAAGCAACACTGGAATTCCACCTTTTTCCAATCCTACACAACCACAACATCGTGCTGATACTAATGATAGCTCTAGTACAAGATCAGGAGACTCACCTACCACCGGAGGTGGGAATGATGAAGGACATAGTGGAGCTGGAGGTAGTGGAGCTGGAGGTAGTGGTGGTGGATATGGAAACTATTATGGACCACCACCTCCCGGATATATGAGCCCCTTCACTGGTGAGGCAAACTTCACGCATGCAACCCAGGATGATGACCATGGCAGTAGGCGGGCAGGACCAGGAATTGGTGCCATAGGGAAGGACTATACTCGCAGAGAAAGAGGCAAAAAGATTTTGTCAAGTCAAGAAGATGACTCGTTATCTAGAACTTCAGACTCTGTTGGATTGGGAAGTAGTAACTATGGTTATCCTTATAACCAACCATTTCCCTACCCTTCATATCCCATTCCTGTTGGGATGGAATCGAGCGACTCATGGAATCAATCCCAGCCTCAATCTTCAAATGATTTTTCTTATGGACAACCTCAACCAATCTCGGATCCATATGGGTGGCATATTAACAATTACATGCAAAACTATTTTGGGGATTTATCATTTGATAACTACTCTTCACAATACACTCATTCTACACATAGAGATGATGAAGATAGTGACAAATTTGAACCTCATAGGAACTCTATGTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

773

Amino Acids

87.22

Weight (kDa)

5.89

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 15 - 166 6.1e-51 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 381 - 449 8e-14 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2043
AccB1I GGYRCC 1 cut(s) 1916
AccB7I CCANNNNNTGG 2 cut(s) 1061, 1913
AccBSI CCGCTC 1 cut(s) 687
AccI GTMKAC 3 cut(s) 207, 1029, 1389
AciI CCGC 2 cut(s) 685, 1897
AclWI GGATC 5 cut(s) 800, 1175, 1588, 2162, 2175
AcoI YGGCCR 1 cut(s) 1063
AcsI RAATTY 4 cut(s) 545, 1356, 1638, 2285
AcuI CTGAAG 4 cut(s) 234, 254, 1545, 1983
AfaI GTAC 3 cut(s) 36, 1020, 1702
AfiI CCNNNNNNNGG 8 cut(s) 821, 989, 1061, 1727, 1734, 1823, 1844, 1913
AflIII ACRYGT 1 cut(s) 751
AgsI TTSAA 8 cut(s) 640, 770, 905, 961, 1355, 1463, 2130, 2291
AhlI ACTAGT 1 cut(s) 653
AjnI CCWGG 2 cut(s) 1872, 1906
AjuI GAANNNNNNNTTGG 4 cut(s) 662, 694, 845, 877
AleI CACNNNNGTG 1 cut(s) 806
Alw21I GWGCWC 1 cut(s) 1265
Alw26I GTCTC 2 cut(s) 1155, 1707
AlwI GGATC 5 cut(s) 800, 1175, 1588, 2162, 2175
AlwNI CAGNNNCTG 2 cut(s) 1133, 2006
AoxI GGCC 4 cut(s) 21, 172, 666, 1063
ApeKI GCWGC 6 cut(s) 416, 944, 947, 1133, 1587, 1615
ApoI RAATTY 4 cut(s) 545, 1356, 1638, 2285
Asp700I GAANNNNTTC 1 cut(s) 564
AspS9I GGNCC 6 cut(s) 168, 173, 666, 1179, 1811, 1904
AsuC2I CCSGG 2 cut(s) 984, 1824
AsuHPI GGTGA 5 cut(s) 1044, 1409, 1439, 1710, 1859
AsuII TTCGAA 1 cut(s) 1602
AvaII GGWCC 4 cut(s) 168, 1179, 1811, 1904
BalI TGGCCA 1 cut(s) 1065
BamHI GGATCC 1 cut(s) 2167
BanI GGYRCC 1 cut(s) 1916
BanII GRGCYC 1 cut(s) 1838
BbsI GAAGAC 1 cut(s) 912
Bbv12I GWGCWC 1 cut(s) 1265
BbvI GCAGC 6 cut(s) 428, 956, 959, 1120, 1599, 1627
BccI CCATC 4 cut(s) 159, 906, 992, 2080
BceAI ACGGC 1 cut(s) 1245
BcgI CGANNNNNNTGC 2 cut(s) 923, 957
BciT130I CCWGG 2 cut(s) 1874, 1908
BciVI GTATCC 1 cut(s) 822
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 2 cut(s) 984, 1824
BcoDI GTCTC 2 cut(s) 1155, 1707
BcuI ACTAGT 1 cut(s) 653
BfaI CTAG 6 cut(s) 489, 654, 1406, 1584, 1698, 1992
BfuI GTATCC 1 cut(s) 822
BisI GCNGC 7 cut(s) 417, 685, 945, 948, 1134, 1588, 1616
BlsI GCNGC 7 cut(s) 418, 686, 946, 949, 1135, 1589, 1617
Bme1390I CCNGG 4 cut(s) 984, 1824, 1874, 1908
Bme18I GGWCC 4 cut(s) 168, 1179, 1811, 1904
BmgT120I GGNCC 6 cut(s) 168, 173, 666, 1179, 1811, 1904
BmiI GGNNCC 3 cut(s) 1180, 1918, 2169
BmrFI CCNGG 4 cut(s) 984, 1824, 1874, 1908
BmsI GCATC 7 cut(s) 235, 397, 528, 831, 1058, 1087, 1174
BpiI GAAGAC 1 cut(s) 912
BpmI CTGGAG 2 cut(s) 1782, 1797
Bpu14I TTCGAA 1 cut(s) 1602
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 2 cut(s) 984, 1824
Bsa29I ATCGAT 1 cut(s) 1380
BsaAI YACGTR 2 cut(s) 1013, 1535
BsaJI CCNNGG 4 cut(s) 213, 1393, 1872, 1885
BsaWI WCCGGW 1 cut(s) 1727
Bsc4I CCNNNNNNNGG 8 cut(s) 821, 989, 1061, 1727, 1734, 1823, 1844, 1913
Bse118I RCCGGY 2 cut(s) 23, 170
Bse1I ACTGG 2 cut(s) 1639, 1849
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 2 cut(s) 1874, 1908
BseCI ATCGAT 1 cut(s) 1380
BseDI CCNNGG 4 cut(s) 213, 1393, 1872, 1885
BseGI GGATG 7 cut(s) 17, 170, 301, 862, 1275, 1882, 2091
BseLI CCNNNNNNNGG 8 cut(s) 821, 989, 1061, 1727, 1734, 1823, 1844, 1913
BseMI GCAATG 1 cut(s) 453
BseNI ACTGG 2 cut(s) 1639, 1849
BseRI GAGGAG 3 cut(s) 570, 1583, 1586
BseXI GCAGC 6 cut(s) 428, 956, 959, 1120, 1599, 1627
BseYI CCCAGC 2 cut(s) 1507, 2115
BsgI GTGCAG 1 cut(s) 435
Bsh1285I CGRYCG 1 cut(s) 1393
BshFI GGCC 4 cut(s) 23, 174, 668, 1065
BshNI GGYRCC 1 cut(s) 1916
BshVI ATCGAT 1 cut(s) 1380
BsiEI CGRYCG 1 cut(s) 1393
BsiHKAI GWGCWC 1 cut(s) 1265
BsiSI CCGG 5 cut(s) 24, 171, 984, 1728, 1824
BslFI GGGAC 1 cut(s) 1192
BslI CCNNNNNNNGG 8 cut(s) 821, 989, 1061, 1727, 1734, 1823, 1844, 1913
BsmAI GTCTC 2 cut(s) 1155, 1707
BsmFI GGGAC 1 cut(s) 1192
BsnI GGCC 4 cut(s) 23, 174, 668, 1065
Bsp119I TTCGAA 1 cut(s) 1602
Bsp1286I GDGCHC 2 cut(s) 1265, 1838
Bsp1407I TGTACA 1 cut(s) 1018
Bsp143I GATC 9 cut(s) 430, 495, 724, 792, 1167, 1381, 1580, 1706, 2167
Bsp19I CCATGG 1 cut(s) 1885
BspACI CCGC 2 cut(s) 685, 1897
BspANI GGCC 4 cut(s) 23, 174, 668, 1065
BspDI ATCGAT 1 cut(s) 1380
BspLI GGNNCC 3 cut(s) 1180, 1918, 2169
BspPI GGATC 5 cut(s) 800, 1175, 1588, 2162, 2175
BspT104I TTCGAA 1 cut(s) 1602
BspT107I GGYRCC 1 cut(s) 1916
BsrBI CCGCTC 1 cut(s) 687
BsrDI GCAATG 1 cut(s) 453
BsrFI RCCGGY 2 cut(s) 23, 170
BsrGI TGTACA 1 cut(s) 1018
BsrI ACTGG 2 cut(s) 1639, 1849
BssAI RCCGGY 2 cut(s) 23, 170
BssECI CCNNGG 4 cut(s) 213, 1393, 1872, 1885
BssMI GATC 9 cut(s) 430, 495, 724, 792, 1167, 1381, 1580, 1706, 2167
BssNAI GTATAC 2 cut(s) 208, 1030
BssT1I CCWWGG 2 cut(s) 213, 1885
Bst1107I GTATAC 2 cut(s) 208, 1030
Bst2UI CCWGG 2 cut(s) 1874, 1908
Bst4CI ACNGT 5 cut(s) 350, 475, 521, 1195, 1394
Bst6I CTCTTC 2 cut(s) 1482, 2242
BstAUI TGTACA 1 cut(s) 1018
BstBAI YACGTR 2 cut(s) 1013, 1535
BstBI TTCGAA 1 cut(s) 1602
BstC8I GCNNGC 3 cut(s) 942, 1866, 1899
BstDSI CCRYGG 2 cut(s) 1393, 1885
BstEII GGTNACC 3 cut(s) 1001, 1397, 1448
BstF5I GGATG 7 cut(s) 17, 170, 301, 862, 1275, 1882, 2091
BstKTI GATC 9 cut(s) 433, 498, 727, 795, 1170, 1384, 1583, 1709, 2170
BstMAI GTCTC 2 cut(s) 1155, 1707
BstMBI GATC 9 cut(s) 430, 495, 724, 792, 1167, 1381, 1580, 1706, 2167
BstMCI CGRYCG 1 cut(s) 1393
BstMWI GCNNNNNNNGC 2 cut(s) 1055, 1529
BstNI CCWGG 2 cut(s) 1874, 1908
BstNSI RCATGY 4 cut(s) 755, 944, 1868, 2200
BstPI GGTNACC 3 cut(s) 1001, 1397, 1448
BstSCI CCNGG 4 cut(s) 982, 1822, 1872, 1906
BstV1I GCAGC 6 cut(s) 428, 956, 959, 1120, 1599, 1627
BstV2I GAAGAC 1 cut(s) 912
BstX2I RGATCY 2 cut(s) 1580, 2167
BstXI CCANNNNNNTGG 1 cut(s) 2178
BstYI RGATCY 2 cut(s) 1580, 2167
BstZ17I GTATAC 2 cut(s) 208, 1030
Bsu15I ATCGAT 1 cut(s) 1380
BsuI GTATCC 1 cut(s) 822
BsuRI GGCC 4 cut(s) 23, 174, 668, 1065
BsuTUI ATCGAT 1 cut(s) 1380
BtgI CCRYGG 2 cut(s) 1393, 1885
BtsCI GGATG 7 cut(s) 17, 170, 301, 862, 1275, 1882, 2091
BtsIMutI CAGTG 3 cut(s) 1590, 1632, 1842
Cac8I GCNNGC 3 cut(s) 942, 1866, 1899
CaiI CAGNNNCTG 2 cut(s) 1133, 2006
Cfr10I RCCGGY 2 cut(s) 23, 170
Cfr13I GGNCC 6 cut(s) 168, 173, 666, 1179, 1811, 1904
ClaI ATCGAT 1 cut(s) 1380
Csp6I GTAC 3 cut(s) 35, 1019, 1701
CviQI GTAC 3 cut(s) 35, 1019, 1701
DpnI GATC 9 cut(s) 432, 497, 726, 794, 1169, 1383, 1582, 1708, 2169
DpnII GATC 9 cut(s) 430, 495, 724, 792, 1167, 1381, 1580, 1706, 2167
DraI TTTAAA 1 cut(s) 1093
EaeI YGGCCR 1 cut(s) 1063
Eam1104I CTCTTC 2 cut(s) 1482, 2242
EarI CTCTTC 2 cut(s) 1482, 2242
Eco130I CCWWGG 2 cut(s) 213, 1885
Eco24I GRGCYC 1 cut(s) 1838
Eco47I GGWCC 4 cut(s) 168, 1179, 1811, 1904
Eco57I CTGAAG 4 cut(s) 234, 254, 1545, 1983
Eco91I GGTNACC 3 cut(s) 1001, 1397, 1448
EcoO65I GGTNACC 3 cut(s) 1001, 1397, 1448
EcoRI GAATTC 1 cut(s) 1638
EcoRII CCWGG 2 cut(s) 1872, 1906
EcoT14I CCWWGG 2 cut(s) 213, 1885
EcoT38I GRGCYC 1 cut(s) 1838
ErhI CCWWGG 2 cut(s) 213, 1885
FaqI GGGAC 1 cut(s) 1192
FauI CCCGC 1 cut(s) 1890
FauNDI CATATG 1 cut(s) 2173
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 3 cut(s) 207, 1029, 1389
Fnu4HI GCNGC 7 cut(s) 417, 685, 945, 948, 1134, 1588, 1616
FokI GGATG 7 cut(s) 24, 177, 308, 869, 1262, 1889, 2098
FriOI GRGCYC 1 cut(s) 1838
Fsp4HI GCNGC 7 cut(s) 417, 685, 945, 948, 1134, 1588, 1616
FspBI CTAG 6 cut(s) 489, 654, 1406, 1584, 1698, 1992
GluI GCNGC 7 cut(s) 417, 685, 945, 948, 1134, 1588, 1616
GsaI CCCAGC 2 cut(s) 1511, 2119
GsuI CTGGAG 2 cut(s) 1782, 1797
HaeIII GGCC 4 cut(s) 23, 174, 668, 1065
HapII CCGG 5 cut(s) 24, 171, 984, 1728, 1824
HincII GTYRAC 4 cut(s) 17, 109, 799, 1390
HindII GTYRAC 4 cut(s) 17, 109, 799, 1390
HindIII AAGCTT 1 cut(s) 1349
HpaI GTTAAC 1 cut(s) 109
HpaII CCGG 5 cut(s) 24, 171, 984, 1728, 1824
HphI GGTGA 5 cut(s) 1044, 1409, 1439, 1710, 1859
Hpy166II GTNNAC 6 cut(s) 17, 109, 208, 799, 1030, 1390
Hpy188I TCNGA 4 cut(s) 234, 253, 2002, 2167
Hpy188III TCNNGA 6 cut(s) 337, 622, 1361, 1710, 1973, 1992
Hpy8I GTNNAC 6 cut(s) 17, 109, 208, 799, 1030, 1390
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 7 cut(s) 847, 1493, 1535, 1739, 1849, 1921, 2073
HpyCH4III ACNGT 5 cut(s) 350, 475, 521, 1195, 1394
HpyCH4IV ACGT 2 cut(s) 1012, 1534
HpyF10VI GCNNNNNNNGC 2 cut(s) 1055, 1529
HpySE526I ACGT 2 cut(s) 1012, 1534
Ksp22I TGATCA 1 cut(s) 724
KspAI GTTAAC 1 cut(s) 109
Kzo9I GATC 9 cut(s) 430, 495, 724, 792, 1167, 1381, 1580, 1706, 2167
LmnI GCTCC 4 cut(s) 1260, 1516, 1757, 1772
Lsp1109I GCAGC 6 cut(s) 428, 956, 959, 1120, 1599, 1627
LweI GCATC 7 cut(s) 235, 397, 528, 831, 1058, 1087, 1174
MaeI CTAG 6 cut(s) 489, 654, 1406, 1584, 1698, 1992
MaeII ACGT 2 cut(s) 1012, 1534
MaeIII GTNAC 8 cut(s) 155, 337, 656, 1001, 1397, 1448, 2024, 2279
MalI GATC 9 cut(s) 432, 497, 726, 794, 1169, 1383, 1582, 1708, 2169
MbiI CCGCTC 1 cut(s) 687
MboI GATC 9 cut(s) 430, 495, 724, 792, 1167, 1381, 1580, 1706, 2167
MflI RGATCY 2 cut(s) 1580, 2167
MhlI GDGCHC 2 cut(s) 1265, 1838
MlsI TGGCCA 1 cut(s) 1065
MluNI TGGCCA 1 cut(s) 1065
MlyI GAGTC 6 cut(s) 748, 1589, 1709, 1976, 1997, 2093
MmeI TCCRAC 3 cut(s) 10, 970, 1990
Mox20I TGGCCA 1 cut(s) 1065
MroXI GAANNNNTTC 1 cut(s) 564
MscI TGGCCA 1 cut(s) 1065
MseI TTAA 5 cut(s) 108, 380, 608, 1092, 2187
MslI CAYNNNNRTG 7 cut(s) 149, 308, 393, 806, 1485, 2176, 2265
Msp20I TGGCCA 1 cut(s) 1065
MspI CCGG 5 cut(s) 24, 171, 984, 1728, 1824
MspR9I CCNGG 4 cut(s) 984, 1824, 1874, 1908
MvaI CCWGG 2 cut(s) 1874, 1908
MwoI GCNNNNNNNGC 2 cut(s) 1055, 1529
NciI CCSGG 2 cut(s) 984, 1824
NcoI CCATGG 1 cut(s) 1885
NdeI CATATG 1 cut(s) 2173
NdeII GATC 9 cut(s) 430, 495, 724, 792, 1167, 1381, 1580, 1706, 2167
NlaIV GGNNCC 3 cut(s) 1180, 1918, 2169
NmuCI GTSAC 5 cut(s) 155, 337, 656, 1397, 2279
NspI RCATGY 4 cut(s) 755, 944, 1868, 2200
NspV TTCGAA 1 cut(s) 1602
OliI CACNNNNGTG 1 cut(s) 806
PaeI GCATGC 2 cut(s) 944, 1868
PciI ACATGT 1 cut(s) 751
PcsI WCGNNNNNNNCGW 1 cut(s) 1386
PdmI GAANNNNTTC 1 cut(s) 564
PfeI GAWTC 4 cut(s) 585, 961, 2090, 2107
PflMI CCANNNNNTGG 2 cut(s) 1061, 1913
PfoI TCCNGGA 1 cut(s) 1822
PkrI GCNGC 7 cut(s) 418, 686, 946, 949, 1135, 1589, 1617
PleI GAGTC 6 cut(s) 747, 1589, 1709, 1976, 1997, 2093
PpsI GAGTC 6 cut(s) 747, 1589, 1709, 1976, 1997, 2093
Ppu21I YACGTR 2 cut(s) 1013, 1535
PscI ACATGT 1 cut(s) 751
PsiI TTATAA 1 cut(s) 2043
Psp6I CCWGG 2 cut(s) 1872, 1906
PspEI GGTNACC 3 cut(s) 1001, 1397, 1448
PspFI CCCAGC 2 cut(s) 1507, 2115
PspGI CCWGG 2 cut(s) 1872, 1906
PspN4I GGNNCC 3 cut(s) 1180, 1918, 2169
PspPI GGNCC 6 cut(s) 168, 173, 666, 1179, 1811, 1904
PstNI CAGNNNCTG 2 cut(s) 1133, 2006
PsuI RGATCY 2 cut(s) 1580, 2167
RsaI GTAC 3 cut(s) 36, 1020, 1702
RsaNI GTAC 3 cut(s) 35, 1019, 1701
RseI CAYNNNNRTG 7 cut(s) 149, 308, 393, 806, 1485, 2176, 2265
SalI GTCGAC 1 cut(s) 1388
SaqAI TTAA 5 cut(s) 108, 380, 608, 1092, 2187
SatI GCNGC 7 cut(s) 417, 685, 945, 948, 1134, 1588, 1616
Sau3AI GATC 9 cut(s) 430, 495, 724, 792, 1167, 1381, 1580, 1706, 2167
Sau96I GGNCC 6 cut(s) 168, 173, 666, 1179, 1811, 1904
SchI GAGTC 6 cut(s) 748, 1589, 1709, 1976, 1997, 2093
ScrFI CCNGG 4 cut(s) 984, 1824, 1874, 1908
SduI GDGCHC 2 cut(s) 1265, 1838
SfaNI GCATC 7 cut(s) 235, 397, 528, 831, 1058, 1087, 1174
SfuI TTCGAA 1 cut(s) 1602
SinI GGWCC 4 cut(s) 168, 1179, 1811, 1904
SmiMI CAYNNNNRTG 7 cut(s) 149, 308, 393, 806, 1485, 2176, 2265
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
SpeI ACTAGT 1 cut(s) 653
SphI GCATGC 2 cut(s) 944, 1868
SsiI CCGC 2 cut(s) 685, 1897
SspI AATATT 1 cut(s) 71
SspMI CTAG 6 cut(s) 489, 654, 1406, 1584, 1698, 1992
StyD4I CCNGG 4 cut(s) 982, 1822, 1872, 1906
StyI CCWWGG 2 cut(s) 213, 1885
TaaI ACNGT 5 cut(s) 350, 475, 521, 1195, 1394
TaiI ACGT 2 cut(s) 1015, 1537
TaqI TCGA 7 cut(s) 583, 1138, 1360, 1380, 1389, 1602, 2093
TaqII GACCGA 3 cut(s) 356, 924, 1196
TatI WGTACW 3 cut(s) 34, 1018, 1700
TauI GCSGC 1 cut(s) 687
TfiI GAWTC 4 cut(s) 585, 961, 2090, 2107
Tru1I TTAA 5 cut(s) 108, 380, 608, 1092, 2187
Tru9I TTAA 5 cut(s) 108, 380, 608, 1092, 2187
TscAI CASTG 3 cut(s) 1597, 1639, 1849
TseFI GTSAC 5 cut(s) 155, 337, 656, 1397, 2279
TseI GCWGC 6 cut(s) 416, 944, 947, 1133, 1587, 1615
Tsp45I GTSAC 5 cut(s) 155, 337, 656, 1397, 2279
TspGWI ACGGA 3 cut(s) 177, 805, 1457
TspRI CASTG 3 cut(s) 1597, 1639, 1849
Van91I CCANNNNNTGG 2 cut(s) 1061, 1913
VpaK11BI GGWCC 4 cut(s) 168, 1179, 1811, 1904
XapI RAATTY 4 cut(s) 545, 1356, 1638, 2285
XbaI TCTAGA 1 cut(s) 1991
XceI RCATGY 4 cut(s) 755, 944, 1868, 2200
XmiI GTMKAC 3 cut(s) 207, 1029, 1389
XmnI GAANNNNTTC 1 cut(s) 564
XspI CTAG 6 cut(s) 489, 654, 1406, 1584, 1698, 1992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.