Rw6G021690

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000220
Physical Location & Seq
Reverse (-)
43149355 .. 43152896
3542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G021690.1

Sequence Viewer

Length: 2628 bp
ATGGCTAGTGGCGGAAGTAGACGTGATCCTGCATGGGAGCACGGAGAACAAATCCCTGATAACAAAAATGGCAGCATATGCAAATATTGTGGGGTTACGATGAAAAGTGGTGGAGTGACACGTCTTAAATACCATCTCAGTGGACTTGATCCAGGAAACAATGTGCAACGATGTGAGGCCGTTCCAACAGAACTTCGTGGAGTCATAATTGGCTCACAAGATGATAACGACGATGAGGTGTATGATGATGATGACATGGCAATTCGTAACTCCAGACAAGCACAATGGGAAAGGGAACACCTCCACAAAGTTCCTAACAGAGGACAATCTTCTGGGTCTAGTAGAGGCATCAACAAATCATTCCAAGCCCGCCAAAAGAGTATTAAAAATCTCTTTTCAAAAGGTTCTGTAAAGGAGGTAATGGGTCGTTTGATTGCAAAGTTTTTCATCCATGGTAATATTCCTGCAGAGAAAGCAGCATGTCCTCATTATCAAAATATGGTGATCGGAATCCAAAGAGCAGGAGAAGGAGTTCAACCCCCCACACCTTATGAGATAAGGAACAAGTATTTGGATATGGAACTTATGGATGCACGATCATGCAATGGATGGTCAGGGCCAAGTAGATTGTCCATCATAAATTTCATGGTCTACTCCAAAGGTACCACTGTTTTTCTCAAGTCAGTTGATTCTTCTGGTGAGAAAAAAGAGCATCACTATATATACAAACTCTTGAAGGAAGTTGTCCGAGAGGTTGGAGAGCACAATGTGGTTCAAGTGGTGACTGACAATGCTATTAATTATGTTAAAGCTGGTAAGAAGTTGATGAAACACCATAATATTTATTGGACTTCTTGTGCAGCTCACTGCATTGATCTCATGTTCGAAGAAATGGGGAAGAAAGAACAAATTGCAAAGGTGATTGACAAAGGTAAAATTATTTCAAAATACATTTACAATCATAATTTGTTGTTGAGCAAGATGCCGGATTTTTGTAAAGGAGAAATTATTCATGCCGCACCAACTCGATTCAAGTCAGGATTGAAGCAATTGTTTTCAAGTGATTGGTGGACCAACAGTAATTTTAGTCGCACTGATCATGGTCGCATTGTTGAAGATATTATATTTGATCAAGTATTTTGGAATCAAGCAGAAAATGTGTGTCAAATATCTGAGCCGCTTTATAAAGTATTACGCGTTGTCGACACAGAAGTCTATCCAACCATGGCTGCTGTTTATGAGCTGATGCGTGTAGTGAAGCAAGAGTTAGAAAGAAAGCATGGAGCAAAATGGGTAGTAAATATTATCAATGATCGACCAGGCGTCGGAGACGATTGTATGCTTATTGAAGCTGTGCATAAAGTTTTTTCTAAATTGGACCCTAACTCTCCAACAATAGGTCAATTCGGAAAAGAGCTTACCTATTTTAAAGATGCAAAACTAAATTTTGGCACGCCAACAACAATTGCAGCCAGAACAGAGATGTCTCCTACCGATTGGTGGGTAATGTATGGAAGTAGTGCACCTACTGTGAGAAAGTTAGCTATTAAAGTATTATCACAAACAGCATCGGCATCTGCCTGTGAAAGGAATTGGAGCACATTTGCACTAATTCATACTAAGCAAAGAAACAAGTTGGGATATGGTAGGCTACAGAAATTGGTATTCTGCTATTATAACATGAGGCTTAAAATTCGAGACAGAGAAGCTGTGCATTCTCAGATGGAACAAAAAGTTCCATTGGATGTCTTTGATATTGGTACTGAGTTTGATGACGACGAGAGTGATCAACTTTATGAATGGATCAGGCCACTTCATTTGGATGACGATGAACACAATCCATCTCCCAGAGTTGCTGAAGAAGCACGTGCAGAAGGAATAAATGTAGAGCAAGTAATTCCAGAAAAGGTTATAGGACCTGATAGAGAATCTATTGAAGCTTTAGTGGGCCGATCTAATCTTTTACCTCGATCCTCTCACAGGGAGTCTCATGGCTCACATATTCCTTCAAGGACAAATGATAGCTCAAGCACAAGATCAGGAGGCTCATCAAGCATCAGAGGTGGAGATAGTGGAAGTTACAGCGGAGGTGGAGGTGATGATAGAGTTGGAGGTTATAATGGAAGTCAAATTGGTCACATGAGCCCATTTACTGGTGAGCCAAACTTCATACATGAAACACAAGATGAATATCATGGGAGTAGGAGAGCAGGATCGGGTATTGGTGCTATAGGAAAACACTATACACCAAGGGAAAGAGGTCAAGGAAGCATGTCAAGGCATGAAGATGATTCTTTATCTCCCACTTTTGACTCATTTGCATACATGACGCCTTATGGGATGCAATCAAGTGACAACTCATGGACACAATCTGAAGGACAATCTTCAAATGATTATGGTTATGGCCAAAGTCGAGCAATGCATGATCCCTACTATAACTACCAGCACCAAAACCCGCAGCCAATCCCCCCAGACCCATATGGTTGGCATGTAAGTCAATACATGCAAAACTACAGAGGGGAGGTATCATTTAATGACTATTCTTCACAATACACTCAAGACTCTACATATAGAGATGATGAAGATAATGAAGATTTTGTACCTCATAGGTCTTCTACGTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

875

Amino Acids

98.94

Weight (kDa)

6.38

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 201 - 314 3.4e-38 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 493 - 561 1.5e-12 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1185, 1677, 2118
AasI GACNNNNNNGTC 1 cut(s) 1211
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 1 cut(s) 662
AccB7I CCANNNNNTGG 1 cut(s) 2153
AccI GTMKAC 3 cut(s) 19, 651, 1203
AccII CGCG 1 cut(s) 1197
AciI CCGC 6 cut(s) 12, 370, 1017, 1178, 2085, 2456
AclWI GGATC 6 cut(s) 20, 143, 1811, 1965, 2221, 2420
AcoI YGGCCR 1 cut(s) 2404
AcsI RAATTY 3 cut(s) 640, 1444, 1692
AcuI CTGAAG 2 cut(s) 1878, 2394
AcvI CACGTG 1 cut(s) 1868
AcyI GRCGYC 2 cut(s) 1323, 2330
AdeI CACNNNGTG 1 cut(s) 769
AfaI GTAC 3 cut(s) 664, 1762, 2601
AfiI CCNNNNNNNGG 7 cut(s) 320, 1325, 1397, 1500, 1587, 1980, 2153
AflIII ACRYGT 2 cut(s) 119, 1195
AhdI GACNNNNNGTC 1 cut(s) 1322
AjiI CACGTC 2 cut(s) 23, 122
AjnI CCWGG 2 cut(s) 151, 1318
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AloI GAACNNNNNNTCC 2 cut(s) 516, 548
AluBI AGCT 9 cut(s) 812, 863, 1243, 1352, 1417, 1544, 1709, 1940, 2025
AluI AGCT 9 cut(s) 812, 863, 1243, 1352, 1417, 1544, 1709, 1940, 2025
Alw21I GWGCWC 4 cut(s) 42, 765, 1525, 1601
Alw26I GTCTC 4 cut(s) 1323, 1491, 1692, 1992
Alw44I GTGCAC 1 cut(s) 1521
AlwI GGATC 6 cut(s) 20, 143, 1811, 1965, 2221, 2420
AoxI GGCC 5 cut(s) 177, 617, 1808, 1948, 2404
ApaLI GTGCAC 1 cut(s) 1521
ApeKI GCWGC 6 cut(s) 72, 476, 860, 1229, 1469, 2458
ApoI RAATTY 3 cut(s) 640, 1444, 1692
AseI ATTAAT 1 cut(s) 798
Asp700I GAANNNNTTC 2 cut(s) 531, 1008
Asp718I GGTACC 1 cut(s) 662
AspS9I GGNCC 5 cut(s) 617, 1071, 1378, 1916, 1948
AsuHPI GGTGA 6 cut(s) 514, 710, 793, 931, 2108, 2168
AsuII TTCGAA 1 cut(s) 885
AvaII GGWCC 3 cut(s) 1071, 1378, 1916
BaeGI GKGCMC 1 cut(s) 1525
BaeI ACNNNNGTAYC 2 cut(s) 654, 687
BalI TGGCCA 1 cut(s) 2406
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 2147
BarI GAAGNNNNNNTAC 2 cut(s) 2583, 2615
BbrPI CACGTG 1 cut(s) 1868
BbsI GAAGAC 1 cut(s) 2604
Bbv12I GWGCWC 4 cut(s) 42, 765, 1525, 1601
BbvI GCAGC 6 cut(s) 84, 488, 872, 1216, 1481, 2470
BccI CCATC 5 cut(s) 141, 603, 641, 1717, 1849
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 2 cut(s) 153, 1320
BclI TGATCA 3 cut(s) 1096, 1129, 1786
BcoDI GTCTC 4 cut(s) 1323, 1491, 1692, 1992
BfaI CTAG 2 cut(s) 6, 339
BfmI CTRYAG 4 cut(s) 465, 1652, 2229, 2512
BisI GCNGC 8 cut(s) 73, 477, 861, 1017, 1178, 1230, 1470, 2459
BlsI GCNGC 8 cut(s) 74, 478, 862, 1018, 1179, 1231, 1471, 2460
Bme1390I CCNGG 2 cut(s) 153, 1320
Bme18I GGWCC 3 cut(s) 1071, 1378, 1916
BmeRI GACNNNNNGTC 1 cut(s) 1322
BmgBI CACGTC 2 cut(s) 23, 122
BmgT120I GGNCC 5 cut(s) 617, 1071, 1378, 1916, 1948
BmiI GGNNCC 2 cut(s) 664, 1380
BmrFI CCNGG 2 cut(s) 153, 1320
BpiI GAAGAC 1 cut(s) 2604
BpmI CTGGAG 1 cut(s) 256
Bpu14I TTCGAA 1 cut(s) 885
BpuEI CTTGAG 3 cut(s) 662, 2011, 2541
BsaAI YACGTR 2 cut(s) 1868, 2619
BsaBI GATNNNNATC 2 cut(s) 509, 2190
BsaHI GRCGYC 2 cut(s) 1323, 2330
BsaJI CCNNGG 3 cut(s) 451, 1224, 2249
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 7 cut(s) 320, 1325, 1397, 1500, 1587, 1980, 2153
Bse1I ACTGG 1 cut(s) 2158
Bse3DI GCAATG 2 cut(s) 610, 2424
Bse8I GATNNNNATC 2 cut(s) 509, 2190
BseBI CCWGG 2 cut(s) 153, 1320
BseDI CCNNGG 3 cut(s) 451, 1224, 2249
BseGI GGATG 6 cut(s) 447, 595, 614, 1750, 1828, 2346
BseJI GATNNNNATC 2 cut(s) 509, 2190
BseLI CCNNNNNNNGG 7 cut(s) 320, 1325, 1397, 1500, 1587, 1980, 2153
BseMI GCAATG 2 cut(s) 610, 2424
BseMII CTCAG 4 cut(s) 151, 1164, 1733, 1755
BseNI ACTGG 1 cut(s) 2158
BseSI GKGCMC 1 cut(s) 1525
BseXI GCAGC 6 cut(s) 84, 488, 872, 1216, 1481, 2470
BsgI GTGCAG 2 cut(s) 879, 1890
Bsh1236I CGCG 1 cut(s) 1197
BshFI GGCC 5 cut(s) 179, 619, 1810, 1950, 2406
BshNI GGYRCC 1 cut(s) 662
BsiHKAI GWGCWC 4 cut(s) 42, 765, 1525, 1601
BsiSI CCGG 1 cut(s) 986
BslI CCNNNNNNNGG 7 cut(s) 320, 1325, 1397, 1500, 1587, 1980, 2153
BsmAI GTCTC 4 cut(s) 1323, 1491, 1692, 1992
BsmBI CGTCTC 1 cut(s) 1323
BsmI GAATGC 1 cut(s) 1714
BsnI GGCC 5 cut(s) 179, 619, 1810, 1950, 2406
Bsp119I TTCGAA 1 cut(s) 885
Bsp1286I GDGCHC 5 cut(s) 42, 765, 1525, 1601, 2147
Bsp19I CCATGG 2 cut(s) 451, 1224
BspACI CCGC 6 cut(s) 12, 370, 1017, 1178, 2085, 2456
BspANI GGCC 5 cut(s) 179, 619, 1810, 1950, 2406
BspCNI CTCAG 4 cut(s) 150, 1165, 1732, 1756
BspFNI CGCG 1 cut(s) 1197
BspLI GGNNCC 2 cut(s) 664, 1380
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 6 cut(s) 20, 143, 1811, 1965, 2221, 2420
BspT104I TTCGAA 1 cut(s) 885
BspT107I GGYRCC 1 cut(s) 662
BsrDI GCAATG 2 cut(s) 610, 2424
BsrI ACTGG 1 cut(s) 2158
BssECI CCNNGG 3 cut(s) 451, 1224, 2249
BssNI GRCGYC 2 cut(s) 1323, 2330
BssT1I CCWWGG 3 cut(s) 451, 1224, 2249
Bst2UI CCWGG 2 cut(s) 153, 1320
Bst4CI ACNGT 3 cut(s) 670, 1079, 1531
BstACI GRCGYC 2 cut(s) 1323, 2330
BstAPI GCANNNNNTGC 1 cut(s) 78
BstBAI YACGTR 2 cut(s) 1868, 2619
BstBI TTCGAA 1 cut(s) 885
BstC8I GCNNGC 2 cut(s) 370, 1454
BstDEI CTNAG 5 cut(s) 137, 1173, 1620, 1719, 1764
BstDSI CCRYGG 2 cut(s) 451, 1224
BstENI CCTNNNNNAGG 3 cut(s) 318, 1585, 1978
BstF5I GGATG 6 cut(s) 447, 595, 614, 1750, 1828, 2346
BstFNI CGCG 1 cut(s) 1197
BstMAI GTCTC 4 cut(s) 1323, 1491, 1692, 1992
BstMWI GCNNNNNNNGC 4 cut(s) 78, 473, 1862, 2052
BstNI CCWGG 2 cut(s) 153, 1320
BstNSI RCATGY 4 cut(s) 483, 2275, 2492, 2506
BstSCI CCNGG 2 cut(s) 151, 1318
BstSFI CTRYAG 4 cut(s) 465, 1652, 2229, 2512
BstSLI GKGCMC 1 cut(s) 1525
BstUI CGCG 1 cut(s) 1197
BstV1I GCAGC 6 cut(s) 84, 488, 872, 1216, 1481, 2470
BstV2I GAAGAC 1 cut(s) 2604
BstXI CCANNNNNNTGG 2 cut(s) 140, 2484
BsuRI GGCC 5 cut(s) 179, 619, 1810, 1950, 2406
BtgI CCRYGG 2 cut(s) 451, 1224
BtrI CACGTC 2 cut(s) 23, 122
BtsCI GGATG 6 cut(s) 447, 595, 614, 1750, 1828, 2346
BtsI GCAGTG 1 cut(s) 865
BtsIMutI CAGTG 4 cut(s) 145, 666, 865, 1092
Cac8I GCNNGC 2 cut(s) 370, 1454
Cfr13I GGNCC 5 cut(s) 617, 1071, 1378, 1916, 1948
CseI GACGC 2 cut(s) 1312, 2338
Csp6I GTAC 3 cut(s) 663, 1761, 2600
CspCI CAANNNNNGTGG 4 cut(s) 70, 105, 1800, 1835
CviQI GTAC 3 cut(s) 663, 1761, 2600
DdeI CTNAG 5 cut(s) 137, 1173, 1620, 1719, 1764
DraI TTTAAA 1 cut(s) 1429
DraIII CACNNNGTG 1 cut(s) 769
DrdI GACNNNNNNGTC 1 cut(s) 1211
DriI GACNNNNNGTC 1 cut(s) 1322
DseDI GACNNNNNNGTC 1 cut(s) 1211
EaeI YGGCCR 1 cut(s) 2404
Eam1105I GACNNNNNGTC 1 cut(s) 1322
EciI GGCGGA 1 cut(s) 27
Eco130I CCWWGG 3 cut(s) 451, 1224, 2249
Eco24I GRGCYC 1 cut(s) 2147
Eco47I GGWCC 3 cut(s) 1071, 1378, 1916
Eco57I CTGAAG 2 cut(s) 1878, 2394
Eco72I CACGTG 1 cut(s) 1868
EcoNI CCTNNNNNAGG 3 cut(s) 318, 1585, 1978
EcoO109I RGGNCCY 1 cut(s) 1916
EcoRII CCWGG 2 cut(s) 151, 1318
EcoT14I CCWWGG 3 cut(s) 451, 1224, 2249
EcoT22I ATGCAT 1 cut(s) 2424
EcoT38I GRGCYC 1 cut(s) 2147
ErhI CCWWGG 3 cut(s) 451, 1224, 2249
Esp3I CGTCTC 1 cut(s) 1323
FauI CCCGC 2 cut(s) 377, 2463
FauNDI CATATG 2 cut(s) 77, 2479
FbaI TGATCA 3 cut(s) 1096, 1129, 1786
FblI GTMKAC 3 cut(s) 19, 651, 1203
Fnu4HI GCNGC 8 cut(s) 73, 477, 861, 1017, 1178, 1230, 1470, 2459
FokI GGATG 6 cut(s) 434, 602, 621, 1757, 1835, 2353
FriOI GRGCYC 1 cut(s) 2147
Fsp4HI GCNGC 8 cut(s) 73, 477, 861, 1017, 1178, 1230, 1470, 2459
FspBI CTAG 2 cut(s) 6, 339
GluI GCNGC 8 cut(s) 73, 477, 861, 1017, 1178, 1230, 1470, 2459
GsuI CTGGAG 1 cut(s) 256
HaeIII GGCC 5 cut(s) 179, 619, 1810, 1950, 2406
HapII CCGG 1 cut(s) 986
HgaI GACGC 2 cut(s) 1312, 2338
Hin1I GRCGYC 2 cut(s) 1323, 2330
HincII GTYRAC 1 cut(s) 1204
HindII GTYRAC 1 cut(s) 1204
HindIII AAGCTT 1 cut(s) 1938
HpaII CCGG 1 cut(s) 986
HphI GGTGA 6 cut(s) 514, 710, 793, 931, 2108, 2168
Hpy166II GTNNAC 6 cut(s) 20, 143, 652, 1071, 1204, 1523
Hpy188I TCNGA 8 cut(s) 509, 749, 1174, 1328, 1409, 1722, 2060, 2374
Hpy188III TCNNGA 7 cut(s) 273, 733, 1038, 1697, 1901, 2040, 2558
Hpy8I GTNNAC 6 cut(s) 20, 143, 652, 1071, 1204, 1523
Hpy99I CGWCG 3 cut(s) 233, 1328, 1781
HpyAV CCTTC 5 cut(s) 521, 730, 1868, 2016, 2369
HpyCH4III ACNGT 3 cut(s) 670, 1079, 1531
HpyCH4IV ACGT 4 cut(s) 22, 121, 1867, 2618
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 473, 1862, 2052
HpyF3I CTNAG 5 cut(s) 137, 1173, 1620, 1719, 1764
HpySE526I ACGT 4 cut(s) 22, 121, 1867, 2618
Hsp92I GRCGYC 2 cut(s) 1323, 2330
KpnI GGTACC 1 cut(s) 666
Ksp22I TGATCA 3 cut(s) 1096, 1129, 1786
LmnI GCTCC 3 cut(s) 37, 1283, 1596
Lsp1109I GCAGC 6 cut(s) 84, 488, 872, 1216, 1481, 2470
MaeI CTAG 2 cut(s) 6, 339
MaeII ACGT 4 cut(s) 22, 121, 1867, 2618
MaeIII GTNAC 7 cut(s) 94, 115, 266, 781, 2078, 2135, 2351
MfeI CAATTG 2 cut(s) 1049, 1464
MhlI GDGCHC 5 cut(s) 42, 765, 1525, 1601, 2147
MlsI TGGCCA 1 cut(s) 2406
MluI ACGCGT 1 cut(s) 1195
MluNI TGGCCA 1 cut(s) 2406
MlyI GAGTC 4 cut(s) 210, 1994, 2306, 2555
MmeI TCCRAC 6 cut(s) 209, 736, 1244, 1306, 1415, 2089
Mox20I TGGCCA 1 cut(s) 2406
Mph1103I ATGCAT 1 cut(s) 2424
MroXI GAANNNNTTC 2 cut(s) 531, 1008
MscI TGGCCA 1 cut(s) 2406
MseI TTAA 8 cut(s) 126, 384, 798, 807, 1428, 1548, 1689, 2532
MslI CAYNNNNRTG 4 cut(s) 138, 598, 1821, 2286
Msp20I TGGCCA 1 cut(s) 2406
MspA1I CMGCKG 1 cut(s) 2085
MspI CCGG 1 cut(s) 986
MspR9I CCNGG 2 cut(s) 153, 1320
MunI CAATTG 2 cut(s) 1049, 1464
Mva1269I GAATGC 1 cut(s) 1714
MvaI CCWGG 2 cut(s) 153, 1320
MvnI CGCG 1 cut(s) 1197
MwoI GCNNNNNNNGC 4 cut(s) 78, 473, 1862, 2052
NcoI CCATGG 2 cut(s) 451, 1224
NdeI CATATG 2 cut(s) 77, 2479
NlaIV GGNNCC 2 cut(s) 664, 1380
NmuCI GTSAC 4 cut(s) 115, 781, 2135, 2351
NsiI ATGCAT 1 cut(s) 2424
NspI RCATGY 4 cut(s) 483, 2275, 2492, 2506
NspV TTCGAA 1 cut(s) 885
PctI GAATGC 1 cut(s) 1714
PdmI GAANNNNTTC 2 cut(s) 531, 1008
PfeI GAWTC 6 cut(s) 510, 689, 1029, 1144, 1928, 2291
PflMI CCANNNNNTGG 1 cut(s) 2153
PfoI TCCNGGA 1 cut(s) 151
PkrI GCNGC 8 cut(s) 74, 478, 862, 1018, 1179, 1231, 1471, 2460
PleI GAGTC 4 cut(s) 209, 1993, 2306, 2555
PmaCI CACGTG 1 cut(s) 1868
PmlI CACGTG 1 cut(s) 1868
PpsI GAGTC 4 cut(s) 209, 1993, 2306, 2555
Ppu21I YACGTR 2 cut(s) 1868, 2619
PpuMI RGGWCCY 1 cut(s) 1916
PshBI ATTAAT 1 cut(s) 798
PsiI TTATAA 3 cut(s) 1185, 1677, 2118
Psp5II RGGWCCY 1 cut(s) 1916
Psp6I CCWGG 2 cut(s) 151, 1318
PspCI CACGTG 1 cut(s) 1868
PspGI CCWGG 2 cut(s) 151, 1318
PspN4I GGNNCC 2 cut(s) 664, 1380
PspPI GGNCC 5 cut(s) 617, 1071, 1378, 1916, 1948
PspPPI RGGWCCY 1 cut(s) 1916
PstI CTGCAG 1 cut(s) 469
RsaI GTAC 3 cut(s) 664, 1762, 2601
RsaNI GTAC 3 cut(s) 663, 1761, 2600
RseI CAYNNNNRTG 4 cut(s) 138, 598, 1821, 2286
SalI GTCGAC 1 cut(s) 1202
SaqAI TTAA 8 cut(s) 126, 384, 798, 807, 1428, 1548, 1689, 2532
SatI GCNGC 8 cut(s) 73, 477, 861, 1017, 1178, 1230, 1470, 2459
Sau96I GGNCC 5 cut(s) 617, 1071, 1378, 1916, 1948
SchI GAGTC 4 cut(s) 210, 1994, 2306, 2555
ScrFI CCNGG 2 cut(s) 153, 1320
SduI GDGCHC 5 cut(s) 42, 765, 1525, 1601, 2147
SfcI CTRYAG 4 cut(s) 465, 1652, 2229, 2512
SfuI TTCGAA 1 cut(s) 885
SinI GGWCC 3 cut(s) 1071, 1378, 1916
SmiMI CAYNNNNRTG 4 cut(s) 138, 598, 1821, 2286
SmlI CTYRAG 3 cut(s) 677, 2026, 2556
SmoI CTYRAG 3 cut(s) 677, 2026, 2556
SsiI CCGC 6 cut(s) 12, 370, 1017, 1178, 2085, 2456
SspI AATATT 4 cut(s) 86, 460, 841, 1303
SspMI CTAG 2 cut(s) 6, 339
StyD4I CCNGG 2 cut(s) 151, 1318
StyI CCWWGG 3 cut(s) 451, 1224, 2249
TaaI ACNGT 3 cut(s) 670, 1079, 1531
TaiI ACGT 4 cut(s) 25, 124, 1870, 2621
TaqI TCGA 7 cut(s) 885, 1027, 1203, 1315, 1696, 1969, 2413
TauI GCSGC 2 cut(s) 1019, 1180
TfiI GAWTC 6 cut(s) 510, 689, 1029, 1144, 1928, 2291
Tru1I TTAA 8 cut(s) 126, 384, 798, 807, 1428, 1548, 1689, 2532
Tru9I TTAA 8 cut(s) 126, 384, 798, 807, 1428, 1548, 1689, 2532
TscAI CASTG 4 cut(s) 145, 673, 872, 1099
TseFI GTSAC 4 cut(s) 115, 781, 2135, 2351
TseI GCWGC 6 cut(s) 72, 476, 860, 1229, 1469, 2458
Tsp45I GTSAC 4 cut(s) 115, 781, 2135, 2351
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 4 cut(s) 145, 673, 872, 1099
Van91I CCANNNNNTGG 1 cut(s) 2153
VneI GTGCAC 1 cut(s) 1521
VpaK11BI GGWCC 3 cut(s) 1071, 1378, 1916
VspI ATTAAT 1 cut(s) 798
XagI CCTNNNNNAGG 3 cut(s) 318, 1585, 1978
XapI RAATTY 3 cut(s) 640, 1444, 1692
XceI RCATGY 4 cut(s) 483, 2275, 2492, 2506
XmiI GTMKAC 3 cut(s) 19, 651, 1203
XmnI GAANNNNTTC 2 cut(s) 531, 1008
XspI CTAG 2 cut(s) 6, 339
Zsp2I ATGCAT 1 cut(s) 2424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.