MD10G1094800.v1.1

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000220
Physical Location & Seq
Forward (+)
14739814 .. 14741377
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1094800.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGTCTCCTACTGAATGGTGGATCATGTATGGGACCGATGCACCAACTGTGAGAAAGTTAGCAATCAAAGTATTATCACAAACAGTTTCCTCATCTGCTTGTGAAAGAAATTGGAGCACATTTGCACTCATACACACAAAGCAAAGAAATAAGTTGGCTCATAGTAGCTTGAAAAAATTAGTTTATTGCTACTACAACATGAAGCTTCAAATTCGAGATAAGGAAGCAGAAATAGATCATGTCGACCGTGGTGACCCACTAGATGTGTTTGATATTGTTGGTGAAGATGATGATACAAAGGGTAACCAACTTTTTCAATGGATTAGACCTCTTCATTTAGATGATGATGAAGGCAACCCAGCTCCCAGAGTTGCTGAAGAAGCACGTAATGAAGGGATAAATGTAGAAAAAGTATTAGAGGAGGAGGTGGGATCTAGCAGCGCTGACTCTTTGGAAGAACTTTTGCGCCCAAAACCAAGAAACATTGGAATTCCACCTTCTTCCAATCCTACACAACCACAACATCGTGCTGATACTAATGATAGCTCTAATACAAGATTAGGAGACTCACCTACCACCGGAGGTGGGAATGATGAAGGAGGTAGTGGAGTTGGAGGTAGTGGTGGTGGTGGATATGGAAACTATTATGGACCACCTCCCGGATTTATAAGCCCCTTCACTGGTGAGGCAAACTTCACGCATGCAACACAGGATGATGACCATGGCAGTAGGCGAGCAAGACCAGGAATTGGTGCCATAGGGAAGGATTATATTCACAGAGAAAGAGGCAAGGAGATTTTGTCAAGTCAAGAAGATGACTCGTTATCTAGAACTTCGGACTCTGTTGGAGTGGGAAGTAGTAACTATGGTAATACTCATAACCAACCATTTCCCTACCCTTCATATCCCATTCCTGTTGGGATGGAATCGAGCGACTCATGGAAACAATCCGAGACTCAATCTTCAAATGATTTTGCTTATGGACAAGGTCAACCAATCTCAGATCCATATGGGTGGCATGTTAACAATTACATACAAAATTATTTTGGGGATTTATCATTTGATAACTACTCTTCACAGTACACTCACTCTACACATAGGGATGATGAAGATAGTCAAAATTTTGAACCTCATAGGAACTCTATGTGGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

385

Amino Acids

42.62

Weight (kDa)

4.87

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimer_Tnp_hAT PF05699 1 - 67 4.8e-13 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 668
AccB1I GGYRCC 1 cut(s) 752
AccB7I CCANNNNNTGG 1 cut(s) 749
AccI GTMKAC 1 cut(s) 243
AclWI GGATC 3 cut(s) 29, 439, 998
AcsI RAATTY 3 cut(s) 210, 489, 1120
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 2 cut(s) 1082, 1151
AfeI AGCGCT 1 cut(s) 442
AfiI CCNNNNNNNGG 5 cut(s) 578, 585, 659, 680, 749
AgsI TTSAA 5 cut(s) 172, 209, 317, 966, 1127
AjnI CCWGG 1 cut(s) 742
AluBI AGCT 4 cut(s) 168, 205, 362, 546
AluI AGCT 4 cut(s) 168, 205, 362, 546
Alw21I GWGCWC 1 cut(s) 119
Alw26I GTCTC 3 cut(s) 9, 558, 947
AlwI GGATC 3 cut(s) 29, 439, 998
Aor51HI AGCGCT 1 cut(s) 442
ApeKI GCWGC 1 cut(s) 438
ApoI RAATTY 3 cut(s) 210, 489, 1120
AspLEI GCGC 2 cut(s) 443, 468
AspS9I GGNCC 2 cut(s) 33, 650
AsuC2I CCSGG 1 cut(s) 660
AsuHPI GGTGA 4 cut(s) 263, 293, 561, 695
AvaII GGWCC 2 cut(s) 33, 650
BanI GGYRCC 1 cut(s) 752
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 1 cut(s) 450
BccI CCATC 1 cut(s) 916
BciT130I CCWGG 1 cut(s) 744
BcnI CCSGG 1 cut(s) 660
BcoDI GTCTC 3 cut(s) 9, 558, 947
BfaI CTAG 3 cut(s) 260, 435, 828
BfoI RGCGCY 1 cut(s) 444
BisI GCNGC 1 cut(s) 439
BlsI GCNGC 1 cut(s) 440
Bme1390I CCNGG 2 cut(s) 660, 744
Bme18I GGWCC 2 cut(s) 33, 650
BmgT120I GGNCC 2 cut(s) 33, 650
BmiI GGNNCC 2 cut(s) 34, 754
BmrFI CCNGG 2 cut(s) 660, 744
BmsI GCATC 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 660
BsaAI YACGTR 1 cut(s) 386
BsaJI CCNNGG 2 cut(s) 247, 721
BsaWI WCCGGW 1 cut(s) 578
BsaXI ACNNNNNCTCC 2 cut(s) 785, 815
Bsc4I CCNNNNNNNGG 5 cut(s) 578, 585, 659, 680, 749
Bse1I ACTGG 1 cut(s) 685
BseBI CCWGG 1 cut(s) 744
BseDI CCNNGG 2 cut(s) 247, 721
BseGI GGATG 3 cut(s) 718, 927, 1108
BseLI CCNNNNNNNGG 5 cut(s) 578, 585, 659, 680, 749
BseMII CTCAG 1 cut(s) 1014
BseNI ACTGG 1 cut(s) 685
BseRI GAGGAG 2 cut(s) 434, 437
BseXI GCAGC 1 cut(s) 450
BseYI CCCAGC 1 cut(s) 358
Bsh1285I CGRYCG 1 cut(s) 247
BshNI GGYRCC 1 cut(s) 752
BsiEI CGRYCG 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 119
BsiSI CCGG 2 cut(s) 579, 660
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 5 cut(s) 578, 585, 659, 680, 749
BsmAI GTCTC 3 cut(s) 9, 558, 947
BsmFI GGGAC 1 cut(s) 46
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 4 cut(s) 21, 235, 431, 1003
Bsp19I CCATGG 1 cut(s) 721
BspCNI CTCAG 1 cut(s) 1013
BspLI GGNNCC 2 cut(s) 34, 754
BspPI GGATC 3 cut(s) 29, 439, 998
BspT107I GGYRCC 1 cut(s) 752
BsrI ACTGG 1 cut(s) 685
BssECI CCNNGG 2 cut(s) 247, 721
BssMI GATC 4 cut(s) 21, 235, 431, 1003
BssT1I CCWWGG 1 cut(s) 721
Bst2UI CCWGG 1 cut(s) 744
Bst4CI ACNGT 4 cut(s) 49, 85, 248, 1080
Bst6I CTCTTC 2 cut(s) 336, 1078
BstBAI YACGTR 1 cut(s) 386
BstC8I GCNNGC 2 cut(s) 702, 735
BstDEI CTNAG 1 cut(s) 1000
BstDSI CCRYGG 2 cut(s) 247, 721
BstEII GGTNACC 2 cut(s) 251, 302
BstF5I GGATG 3 cut(s) 718, 927, 1108
BstH2I RGCGCY 1 cut(s) 444
BstHHI GCGC 2 cut(s) 443, 468
BstKTI GATC 4 cut(s) 24, 238, 434, 1006
BstMAI GTCTC 3 cut(s) 9, 558, 947
BstMBI GATC 4 cut(s) 21, 235, 431, 1003
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstNI CCWGG 1 cut(s) 744
BstNSI RCATGY 2 cut(s) 704, 1022
BstPI GGTNACC 2 cut(s) 251, 302
BstSCI CCNGG 2 cut(s) 658, 742
BstV1I GCAGC 1 cut(s) 450
BstX2I RGATCY 2 cut(s) 431, 1003
BstXI CCANNNNNNTGG 1 cut(s) 1014
BstYI RGATCY 2 cut(s) 431, 1003
BtgI CCRYGG 2 cut(s) 247, 721
BtsCI GGATG 3 cut(s) 718, 927, 1108
BtsIMutI CAGTG 1 cut(s) 678
Cac8I GCNNGC 2 cut(s) 702, 735
CfoI GCGC 2 cut(s) 443, 468
Cfr13I GGNCC 2 cut(s) 33, 650
Csp6I GTAC 2 cut(s) 1081, 1150
CviAII CATG 7 cut(s) 25, 199, 239, 701, 722, 939, 1019
CviJI RGCY 6 cut(s) 158, 168, 205, 362, 546, 672
CviKI_1 RGCY 6 cut(s) 158, 168, 205, 362, 546, 672
CviQI GTAC 2 cut(s) 1081, 1150
DdeI CTNAG 1 cut(s) 1000
DpnI GATC 4 cut(s) 23, 237, 433, 1005
DpnII GATC 4 cut(s) 21, 235, 431, 1003
Eam1104I CTCTTC 2 cut(s) 336, 1078
EarI CTCTTC 2 cut(s) 336, 1078
Eco130I CCWWGG 1 cut(s) 721
Eco47I GGWCC 2 cut(s) 33, 650
Eco47III AGCGCT 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 396
Eco91I GGTNACC 2 cut(s) 251, 302
EcoO65I GGTNACC 2 cut(s) 251, 302
EcoRI GAATTC 1 cut(s) 489
EcoRII CCWGG 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 721
ErhI CCWWGG 1 cut(s) 721
FaeI CATG 7 cut(s) 28, 202, 242, 704, 725, 942, 1022
FaqI GGGAC 1 cut(s) 46
FatI CATG 7 cut(s) 24, 198, 238, 700, 721, 938, 1018
FauNDI CATATG 1 cut(s) 1009
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 1 cut(s) 439
FokI GGATG 3 cut(s) 725, 934, 1115
Fsp4HI GCNGC 1 cut(s) 439
FspBI CTAG 3 cut(s) 260, 435, 828
GlaI GCGC 2 cut(s) 442, 467
GluI GCNGC 1 cut(s) 439
GsaI CCCAGC 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 444
HapII CCGG 2 cut(s) 579, 660
HhaI GCGC 2 cut(s) 443, 468
Hin1II CATG 7 cut(s) 28, 202, 242, 704, 725, 942, 1022
Hin6I GCGC 2 cut(s) 441, 466
HinP1I GCGC 2 cut(s) 441, 466
HincII GTYRAC 3 cut(s) 244, 992, 1024
HindII GTYRAC 3 cut(s) 244, 992, 1024
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 7 cut(s) 446, 566, 818, 839, 926, 935, 955
HpaI GTTAAC 1 cut(s) 1024
HpaII CCGG 2 cut(s) 579, 660
HphI GGTGA 4 cut(s) 263, 293, 561, 695
Hpy166II GTNNAC 4 cut(s) 244, 992, 1024, 1083
Hpy188I TCNGA 3 cut(s) 838, 952, 1003
Hpy188III TCNNGA 3 cut(s) 215, 809, 828
Hpy8I GTNNAC 4 cut(s) 244, 992, 1024, 1083
HpyAV CCTTC 7 cut(s) 344, 386, 507, 590, 685, 757, 909
HpyCH4III ACNGT 4 cut(s) 49, 85, 248, 1080
HpyCH4IV ACGT 1 cut(s) 385
HpyCH4V TGCA 3 cut(s) 41, 125, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
HpyF3I CTNAG 1 cut(s) 1000
HpySE526I ACGT 1 cut(s) 385
Hsp92II CATG 7 cut(s) 28, 202, 242, 704, 725, 942, 1022
HspAI GCGC 2 cut(s) 441, 466
KspAI GTTAAC 1 cut(s) 1024
Kzo9I GATC 4 cut(s) 21, 235, 431, 1003
LmnI GCTCC 2 cut(s) 114, 367
LpnPI CCDG 9 cut(s) 372, 379, 592, 666, 673, 695, 729, 756, 927
Lsp1109I GCAGC 1 cut(s) 450
LweI GCATC 1 cut(s) 28
MaeI CTAG 3 cut(s) 260, 435, 828
MaeII ACGT 1 cut(s) 385
MaeIII GTNAC 3 cut(s) 251, 302, 860
MalI GATC 4 cut(s) 23, 237, 433, 1005
MboI GATC 4 cut(s) 21, 235, 431, 1003
MboII GAAGA 9 cut(s) 296, 323, 389, 467, 492, 824, 954, 1065, 1121
MflI RGATCY 2 cut(s) 431, 1003
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 8 cut(s) 109, 176, 210, 489, 747, 1027, 1039, 1120
MlyI GAGTC 6 cut(s) 440, 560, 812, 833, 929, 949
MmeI TCCRAC 2 cut(s) 592, 826
MseI TTAA 1 cut(s) 1023
MslI CAYNNNNRTG 3 cut(s) 339, 1012, 1101
MspI CCGG 2 cut(s) 579, 660
MspR9I CCNGG 2 cut(s) 660, 744
MvaI CCWGG 1 cut(s) 744
MwoI GCNNNNNNNGC 1 cut(s) 380
NciI CCSGG 1 cut(s) 660
NcoI CCATGG 1 cut(s) 721
NdeI CATATG 1 cut(s) 1009
NdeII GATC 4 cut(s) 21, 235, 431, 1003
NlaIII CATG 7 cut(s) 28, 202, 242, 704, 725, 942, 1022
NlaIV GGNNCC 2 cut(s) 34, 754
NmuCI GTSAC 1 cut(s) 251
NspI RCATGY 2 cut(s) 704, 1022
PaeI GCATGC 1 cut(s) 704
PfeI GAWTC 1 cut(s) 926
PflFI GACNNNGTC 1 cut(s) 987
PflMI CCANNNNNTGG 1 cut(s) 749
PfoI TCCNGGA 1 cut(s) 658
PkrI GCNGC 1 cut(s) 440
PleI GAGTC 6 cut(s) 440, 560, 812, 833, 929, 949
PpsI GAGTC 6 cut(s) 440, 560, 812, 833, 929, 949
Ppu21I YACGTR 1 cut(s) 386
PsiI TTATAA 1 cut(s) 668
Psp6I CCWGG 1 cut(s) 742
PspEI GGTNACC 2 cut(s) 251, 302
PspFI CCCAGC 1 cut(s) 358
PspGI CCWGG 1 cut(s) 742
PspN4I GGNNCC 2 cut(s) 34, 754
PspPI GGNCC 2 cut(s) 33, 650
PsuI RGATCY 2 cut(s) 431, 1003
PsyI GACNNNGTC 1 cut(s) 987
RsaI GTAC 2 cut(s) 1082, 1151
RsaNI GTAC 2 cut(s) 1081, 1150
RseI CAYNNNNRTG 3 cut(s) 339, 1012, 1101
SalI GTCGAC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 1023
SatI GCNGC 1 cut(s) 439
Sau3AI GATC 4 cut(s) 21, 235, 431, 1003
Sau96I GGNCC 2 cut(s) 33, 650
SchI GAGTC 6 cut(s) 440, 560, 812, 833, 929, 949
ScrFI CCNGG 2 cut(s) 660, 744
SduI GDGCHC 1 cut(s) 119
SfaNI GCATC 1 cut(s) 28
SinI GGWCC 2 cut(s) 33, 650
SmiMI CAYNNNNRTG 3 cut(s) 339, 1012, 1101
SphI GCATGC 1 cut(s) 704
Sse9I AATT 8 cut(s) 109, 176, 210, 489, 747, 1027, 1039, 1120
SspMI CTAG 3 cut(s) 260, 435, 828
StyD4I CCNGG 2 cut(s) 658, 742
StyI CCWWGG 1 cut(s) 721
TaaI ACNGT 4 cut(s) 49, 85, 248, 1080
TaiI ACGT 1 cut(s) 388
TaqI TCGA 3 cut(s) 214, 243, 929
TaqII GACCGA 1 cut(s) 50
TasI AATT 8 cut(s) 109, 176, 210, 489, 747, 1027, 1039, 1120
TatI WGTACW 1 cut(s) 1080
TfiI GAWTC 1 cut(s) 926
Tru1I TTAA 1 cut(s) 1023
Tru9I TTAA 1 cut(s) 1023
TscAI CASTG 1 cut(s) 685
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 1 cut(s) 438
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 7 cut(s) 215, 323, 363, 405, 609, 891, 1122
TspRI CASTG 1 cut(s) 685
Tth111I GACNNNGTC 1 cut(s) 987
Van91I CCANNNNNTGG 1 cut(s) 749
VpaK11BI GGWCC 2 cut(s) 33, 650
XapI RAATTY 3 cut(s) 210, 489, 1120
XbaI TCTAGA 1 cut(s) 827
XceI RCATGY 2 cut(s) 704, 1022
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 3 cut(s) 260, 435, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.