MD00G1003900.v1.1

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000220
Physical Location & Seq
Forward (+)
648231 .. 649638
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1003900.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGGACCTGAAGAACGACGCAGTTTGAAACAAGCATTACGTGCCTCCAAACAGTCAGCATGGGAAAGAGAACACCTTCATAAAATTCCTAATAGGGGACAAGGTTCCGGGACAAGTGGTGGTGCACAAATGAGACGGGGAGGCAGTCTTAGAGAATCACAACCAACACCACCAATAGCCCCAAGTTTATATAAGTCATCAAACGCACGTCAAAAGAGTGTTTGGAGTTATTTCAAGGGAGGTAATGTGAAGGAGGGAATGGGGCGTCTAATTAGCAAGTTCTTTATCTATGAAAATGTCCCTGCTGCGAAGGCTTCATCACATCATTTCAAAAATATGGTAGTGGGATGTCAACAGGCCGGTGTTGGAGTACAACCTCCCACTCCCTATGAGATAAGAAACAAATATTTGGATATGGAGTATAAAGACATTGGCGAGTATGTTAACAAGTTGAGGTCAAAGTGGGAAACTAATGGTTGCACAATCATGTGTGACGGATGGACCGGCCCGACCAGATTATCATCTATCATCAACTTCATGGTATACTCCAAGGGCAAGACAATTTTTCTGAAGTCTGTTGATGCTTCAGACCATATAAAGAATTACAAGTATATTTACAAATTATTGAGGGATGTAATCATGGAGGTGGGAGAGCATAATGTTGTCCAAGTCGTGACCGACAACGGTTCTGCATTTGTCAAAGCTGGAAAAAAGTTAATGAAGCATCATAATGTGTTTTGGACATCATGTGCAGCACATTGTATTGATCTTATGTTTGAGGCAATGGGAAAGAGAGAGAATGTTGCTACTGTGGTCAAAAGAGCTAGAACGATCACTAATTATATTTACAATCACGGTTGGTTGTTGGCAAAGATGCGTGAATTTTGCAGAGGAGAAATTATTCGTCCAGCTACCACTCGATTCGCCACCAACTATATTGCATTAAACAGCCTACTCAAGAAGAAAGCAGGGTTGAAGCAACTATTCACTAGTGACGATTGGGCCAACCACAATTTCAGCCGCTCAAATACAGGTCGTATGGTGGAAAGTATAGTGCTTGATCATGCTTTTTGGAGTCAAACAGAACATGTGTGTCAAGTGTTTGAACCTCTTTACAAAGTTTTACGGATCGTTGACACAGAAGTGTATCCTACTATGGGGGCTGTATATGAATTGATGCGTGTAGTGAAGGATGAATTGGAAAGAAAACATGGTGCAAGGTGGGTCGTAAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

413

Amino Acids

47.0

Weight (kDa)

9.74

Isoelectric Point (pI)

29.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 127 - 278 1.6e-52 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1023
AccI GTMKAC 1 cut(s) 543
AciI CCGC 1 cut(s) 1021
AclWI GGATC 1 cut(s) 1136
AcsI RAATTY 2 cut(s) 84, 881
AcuI CTGAAG 3 cut(s) 30, 570, 590
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 1 cut(s) 372
AfiI CCNNNNNNNGG 2 cut(s) 95, 1157
AflIII ACRYGT 1 cut(s) 1087
AgsI TTSAA 5 cut(s) 28, 235, 331, 976, 1106
AhlI ACTAGT 1 cut(s) 989
AjiI CACGTC 1 cut(s) 209
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 1181, 1213
AleI CACNNNNGTG 1 cut(s) 1142
AluBI AGCT 3 cut(s) 704, 824, 911
AluI AGCT 3 cut(s) 704, 824, 911
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 127
Alw44I GTGCAC 1 cut(s) 123
AlwI GGATC 1 cut(s) 1136
AoxI GGCC 3 cut(s) 357, 505, 1002
ApaLI GTGCAC 1 cut(s) 123
ApeKI GCWGC 2 cut(s) 305, 752
ApoI RAATTY 2 cut(s) 84, 881
Asp700I GAANNNNTTC 2 cut(s) 75, 900
AspS9I GGNCC 4 cut(s) 5, 501, 506, 1002
AsuC2I CCSGG 1 cut(s) 109
AvaII GGWCC 2 cut(s) 5, 501
BaeGI GKGCMC 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 2 cut(s) 292, 764
BccI CCATC 1 cut(s) 492
BciVI GTATCC 1 cut(s) 1158
BclI TGATCA 1 cut(s) 1060
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 127
BcuI ACTAGT 1 cut(s) 989
BfaI CTAG 2 cut(s) 825, 990
BfuI GTATCC 1 cut(s) 1158
BisI GCNGC 3 cut(s) 306, 753, 1021
BlsI GCNGC 3 cut(s) 307, 754, 1022
Bme1390I CCNGG 1 cut(s) 109
Bme18I GGWCC 2 cut(s) 5, 501
BmgBI CACGTC 1 cut(s) 209
BmgT120I GGNCC 4 cut(s) 5, 501, 506, 1002
BmiI GGNNCC 2 cut(s) 6, 106
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 4 cut(s) 571, 733, 864, 1167
BpuEI CTTGAG 1 cut(s) 941
BpuMI CCSGG 1 cut(s) 109
BsaAI YACGTR 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 520
BsaHI GRCGYC 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 549
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 1157
Bse118I RCCGGY 2 cut(s) 359, 503
Bse3DI GCAATG 1 cut(s) 789
Bse8I GATNNNNATC 1 cut(s) 520
BseDI CCNNGG 1 cut(s) 549
BseGI GGATG 4 cut(s) 353, 503, 637, 1198
BseJI GATNNNNATC 1 cut(s) 520
BseLI CCNNNNNNNGG 2 cut(s) 95, 1157
BseMI GCAATG 1 cut(s) 789
BseRI GAGGAG 1 cut(s) 906
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 2 cut(s) 292, 764
BsgI GTGCAG 1 cut(s) 771
BshFI GGCC 3 cut(s) 359, 507, 1004
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 3 cut(s) 108, 360, 504
BslFI GGGAC 4 cut(s) 18, 111, 124, 284
BslI CCNNNNNNNGG 2 cut(s) 95, 1157
BsmAI GTCTC 1 cut(s) 127
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 4 cut(s) 18, 111, 124, 284
BsnI GGCC 3 cut(s) 359, 507, 1004
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 4 cut(s) 766, 831, 1060, 1128
BspACI CCGC 1 cut(s) 1021
BspANI GGCC 3 cut(s) 359, 507, 1004
BspLI GGNNCC 2 cut(s) 6, 106
BspPI GGATC 1 cut(s) 1136
BsrBI CCGCTC 1 cut(s) 1023
BsrDI GCAATG 1 cut(s) 789
BsrFI RCCGGY 2 cut(s) 359, 503
BssAI RCCGGY 2 cut(s) 359, 503
BssECI CCNNGG 1 cut(s) 549
BssMI GATC 4 cut(s) 766, 831, 1060, 1128
BssNAI GTATAC 1 cut(s) 544
BssNI GRCGYC 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 549
Bst1107I GTATAC 1 cut(s) 544
Bst4CI ACNGT 4 cut(s) 54, 686, 811, 857
BstACI GRCGYC 1 cut(s) 265
BstAPI GCANNNNNTGC 1 cut(s) 41
BstBAI YACGTR 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 4 cut(s) 353, 503, 637, 1198
BstKTI GATC 4 cut(s) 769, 834, 1063, 1131
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 4 cut(s) 766, 831, 1060, 1128
BstMWI GCNNNNNNNGC 2 cut(s) 41, 311
BstNSI RCATGY 1 cut(s) 1091
BstSCI CCNGG 1 cut(s) 107
BstSLI GKGCMC 1 cut(s) 127
BstV1I GCAGC 2 cut(s) 292, 764
BstZ17I GTATAC 1 cut(s) 544
BsuI GTATCC 1 cut(s) 1158
BsuRI GGCC 3 cut(s) 359, 507, 1004
BtrI CACGTC 1 cut(s) 209
BtsCI GGATG 4 cut(s) 353, 503, 637, 1198
Cfr10I RCCGGY 2 cut(s) 359, 503
Cfr13I GGNCC 4 cut(s) 5, 501, 506, 1002
CseI GACGC 2 cut(s) 27, 254
Csp6I GTAC 1 cut(s) 371
CviAII CATG 8 cut(s) 60, 487, 538, 640, 747, 1064, 1088, 1211
CviQI GTAC 1 cut(s) 371
DdeI CTNAG 1 cut(s) 149
DpnI GATC 4 cut(s) 768, 833, 1062, 1130
DpnII GATC 4 cut(s) 766, 831, 1060, 1128
Eco130I CCWWGG 1 cut(s) 549
Eco47I GGWCC 2 cut(s) 5, 501
Eco57I CTGAAG 3 cut(s) 30, 570, 590
EcoO109I RGGNCCY 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 549
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 8 cut(s) 63, 490, 541, 643, 750, 1067, 1091, 1214
FaqI GGGAC 4 cut(s) 18, 111, 124, 284
FatI CATG 8 cut(s) 59, 486, 537, 639, 746, 1063, 1087, 1210
FbaI TGATCA 1 cut(s) 1060
FblI GTMKAC 1 cut(s) 543
Fnu4HI GCNGC 3 cut(s) 306, 753, 1021
FokI GGATG 4 cut(s) 360, 510, 644, 1205
Fsp4HI GCNGC 3 cut(s) 306, 753, 1021
FspBI CTAG 2 cut(s) 825, 990
GluI GCNGC 3 cut(s) 306, 753, 1021
HaeIII GGCC 3 cut(s) 359, 507, 1004
HapII CCGG 3 cut(s) 108, 360, 504
HgaI GACGC 2 cut(s) 27, 254
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 8 cut(s) 63, 490, 541, 643, 750, 1067, 1091, 1214
HincII GTYRAC 3 cut(s) 353, 445, 1135
HindII GTYRAC 3 cut(s) 353, 445, 1135
HinfI GANTC 3 cut(s) 155, 921, 1075
HpaI GTTAAC 1 cut(s) 445
HpaII CCGG 3 cut(s) 108, 360, 504
Hpy166II GTNNAC 5 cut(s) 125, 353, 445, 544, 1135
Hpy188I TCNGA 2 cut(s) 570, 589
Hpy188III TCNNGA 2 cut(s) 673, 958
Hpy8I GTNNAC 5 cut(s) 125, 353, 445, 544, 1135
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 4 cut(s) 86, 244, 304, 1183
HpyCH4III ACNGT 4 cut(s) 54, 686, 811, 857
HpyCH4IV ACGT 2 cut(s) 40, 208
HpyCH4V TGCA 7 cut(s) 125, 480, 692, 752, 888, 941, 1217
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 311
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 2 cut(s) 40, 208
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 8 cut(s) 63, 490, 541, 643, 750, 1067, 1091, 1214
Ksp22I TGATCA 1 cut(s) 1060
KspAI GTTAAC 1 cut(s) 445
Kzo9I GATC 4 cut(s) 766, 831, 1060, 1128
Lsp1109I GCAGC 2 cut(s) 292, 764
LweI GCATC 4 cut(s) 571, 733, 864, 1167
MaeI CTAG 2 cut(s) 825, 990
MaeII ACGT 2 cut(s) 40, 208
MaeIII GTNAC 3 cut(s) 491, 673, 992
MalI GATC 4 cut(s) 768, 833, 1062, 1130
MbiI CCGCTC 1 cut(s) 1023
MboI GATC 4 cut(s) 766, 831, 1060, 1128
MboII GAAGA 2 cut(s) 23, 973
MhlI GDGCHC 1 cut(s) 127
MlyI GAGTC 1 cut(s) 1084
MmeI TCCRAC 1 cut(s) 346
MroXI GAANNNNTTC 2 cut(s) 75, 900
MseI TTAA 3 cut(s) 444, 716, 944
MslI CAYNNNNRTG 4 cut(s) 485, 644, 729, 1142
MspI CCGG 3 cut(s) 108, 360, 504
MspR9I CCNGG 1 cut(s) 109
MwoI GCNNNNNNNGC 2 cut(s) 41, 311
NciI CCSGG 1 cut(s) 109
NdeII GATC 4 cut(s) 766, 831, 1060, 1128
NlaIII CATG 8 cut(s) 63, 490, 541, 643, 750, 1067, 1091, 1214
NlaIV GGNNCC 2 cut(s) 6, 106
NmuCI GTSAC 3 cut(s) 491, 673, 992
NspI RCATGY 1 cut(s) 1091
OliI CACNNNNGTG 1 cut(s) 1142
PciI ACATGT 1 cut(s) 1087
PdmI GAANNNNTTC 2 cut(s) 75, 900
PfeI GAWTC 2 cut(s) 155, 921
PfoI TCCNGGA 1 cut(s) 107
PkrI GCNGC 3 cut(s) 307, 754, 1022
PleI GAGTC 1 cut(s) 1083
PpsI GAGTC 1 cut(s) 1083
Ppu21I YACGTR 1 cut(s) 41
PpuMI RGGWCCY 1 cut(s) 5
PscI ACATGT 1 cut(s) 1087
Psp5II RGGWCCY 1 cut(s) 5
PspN4I GGNNCC 2 cut(s) 6, 106
PspPI GGNCC 4 cut(s) 5, 501, 506, 1002
PspPPI RGGWCCY 1 cut(s) 5
RsaI GTAC 1 cut(s) 372
RsaNI GTAC 1 cut(s) 371
RseI CAYNNNNRTG 4 cut(s) 485, 644, 729, 1142
SaqAI TTAA 3 cut(s) 444, 716, 944
SatI GCNGC 3 cut(s) 306, 753, 1021
Sau3AI GATC 4 cut(s) 766, 831, 1060, 1128
Sau96I GGNCC 4 cut(s) 5, 501, 506, 1002
SchI GAGTC 1 cut(s) 1084
ScrFI CCNGG 1 cut(s) 109
SduI GDGCHC 1 cut(s) 127
SfaNI GCATC 4 cut(s) 571, 733, 864, 1167
SinI GGWCC 2 cut(s) 5, 501
SmiMI CAYNNNNRTG 4 cut(s) 485, 644, 729, 1142
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
SpeI ACTAGT 1 cut(s) 989
SsiI CCGC 1 cut(s) 1021
SspI AATATT 1 cut(s) 407
SspMI CTAG 2 cut(s) 825, 990
StyD4I CCNGG 1 cut(s) 107
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 4 cut(s) 54, 686, 811, 857
TaiI ACGT 2 cut(s) 43, 211
TaqI TCGA 1 cut(s) 919
TaqII GACCGA 1 cut(s) 692
TatI WGTACW 1 cut(s) 370
TauI GCSGC 1 cut(s) 1023
TfiI GAWTC 2 cut(s) 155, 921
Tru1I TTAA 3 cut(s) 444, 716, 944
Tru9I TTAA 3 cut(s) 444, 716, 944
TseFI GTSAC 3 cut(s) 491, 673, 992
TseI GCWGC 2 cut(s) 305, 752
Tsp45I GTSAC 3 cut(s) 491, 673, 992
TspDTI ATGAA 7 cut(s) 68, 306, 306, 526, 734, 1185, 1209
TspGWI ACGGA 2 cut(s) 510, 1141
VneI GTGCAC 1 cut(s) 123
VpaK11BI GGWCC 2 cut(s) 5, 501
XapI RAATTY 2 cut(s) 84, 881
XceI RCATGY 1 cut(s) 1091
XmiI GTMKAC 1 cut(s) 543
XmnI GAANNNNTTC 2 cut(s) 75, 900
XspI CTAG 2 cut(s) 825, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.