MD13G1176700.v1.1

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000220
Physical Location & Seq
Reverse (-)
14648323 .. 14649257
935 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1176700.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGAGGTGGGAGAGCATAATGTTGTCCAAGTCGTGACCGACAACGGTTCTGCATTTGTCAAAGCTGGAAAAAAGTTAATGAAGCATCATAATGTGTTTTGGACATCATGTGCAGCACATTGTATTGATCTCATGTTTGAGGCAATGGGGAAGAGAGAGAATGTTGCTACTGTGGTCAAAAGAGCTAGAACGATCACAAATTATATTTACAATCACGGTTGGTTGTTGGCAAAGATGCGTGAATTTTGCAAAGGAGAAATCATTCGTCCAGCTACCACTCGATTCGCCACCAACTATATTGCATTAGACAGCCTATTGAAGAAGAAAGCAGGGTTGAAGCAACTATTCACTAGTGACGATTGGGCCAACCACAATTTCAGCCGCTCAAATACAGGTCGTATGGTGGAAAGTATAGTGCTTGATCATGCTTTCTGGACTCAATCAGAACATGTGTGCCAACTGTTTGAACCTCTTTACAAAGTTTTACGGATCGTTGACACAGAAGTGTATCCTACTATGGGGGCAGTATATGAGTTGATGCGTGTAGTGAAGGATGAATTGGAAAGAAAACATGGTGCAAGATGGGTCATCAAGATAATTGAAGATCGATGGTATAAAACATTGTACCACGATTTGCATGCAGCAGCCTATTATTTGAATCCTCGATACCAATACAGACCCGGTGTTGGAGATGATGGTACCCTTATACGTGCTGTACATAATGTATACTCTAAATTAGACCCTGCATCACCAGCAGTTGGCCAATTTGGAAATGAGGTACACAATTACTTAAATTATAATAATGACTTAAATTATAATATTAATAATTACTTTGTTGGATTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

283

Amino Acids

32.6

Weight (kDa)

8.86

Isoelectric Point (pI)

24.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 2 - 65 1.9e-19 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 798, 816
Acc65I GGTACC 1 cut(s) 698
AccB1I GGYRCC 1 cut(s) 698
AccB7I CCANNNNNTGG 1 cut(s) 758
AccBSI CCGCTC 1 cut(s) 384
AccI GTMKAC 1 cut(s) 726
AciI CCGC 1 cut(s) 382
AclWI GGATC 1 cut(s) 497
AcoI YGGCCR 1 cut(s) 760
AcsI RAATTY 1 cut(s) 242
AfaI GTAC 4 cut(s) 626, 700, 717, 780
AfiI CCNNNNNNNGG 3 cut(s) 518, 686, 758
AflIII ACRYGT 1 cut(s) 448
AgsI TTSAA 5 cut(s) 319, 337, 467, 602, 658
AhlI ACTAGT 1 cut(s) 350
AjuI GAANNNNNNNTTGG 4 cut(s) 359, 391, 542, 574
AleI CACNNNNGTG 1 cut(s) 503
AluBI AGCT 3 cut(s) 65, 185, 272
AluI AGCT 3 cut(s) 65, 185, 272
AlwI GGATC 1 cut(s) 497
AoxI GGCC 2 cut(s) 363, 760
ApeKI GCWGC 3 cut(s) 113, 641, 644
ApoI RAATTY 1 cut(s) 242
AseI ATTAAT 1 cut(s) 822
Asp700I GAANNNNTTC 1 cut(s) 261
Asp718I GGTACC 1 cut(s) 698
AspS9I GGNCC 1 cut(s) 363
AsuC2I CCSGG 1 cut(s) 681
AsuHPI GGTGA 1 cut(s) 741
BalI TGGCCA 1 cut(s) 762
BanI GGYRCC 1 cut(s) 698
BbvI GCAGC 3 cut(s) 125, 653, 656
BccI CCATC 3 cut(s) 576, 603, 689
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BciVI GTATCC 1 cut(s) 519
BclI TGATCA 1 cut(s) 421
BcnI CCSGG 1 cut(s) 681
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 2 cut(s) 186, 351
BfuI GTATCC 1 cut(s) 519
BisI GCNGC 4 cut(s) 114, 382, 642, 645
BlsI GCNGC 4 cut(s) 115, 383, 643, 646
Bme1390I CCNGG 1 cut(s) 681
BmgT120I GGNCC 1 cut(s) 363
BmiI GGNNCC 1 cut(s) 700
BmrFI CCNGG 1 cut(s) 681
BmsI GCATC 4 cut(s) 94, 225, 528, 755
BpuMI CCSGG 1 cut(s) 681
Bsa29I ATCGAT 1 cut(s) 607
BsaAI YACGTR 1 cut(s) 710
Bsc4I CCNNNNNNNGG 3 cut(s) 518, 686, 758
Bse3DI GCAATG 1 cut(s) 150
BseCI ATCGAT 1 cut(s) 607
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 3 cut(s) 518, 686, 758
BseMI GCAATG 1 cut(s) 150
BseXI GCAGC 3 cut(s) 125, 653, 656
BsgI GTGCAG 1 cut(s) 132
BshFI GGCC 2 cut(s) 365, 762
BshNI GGYRCC 1 cut(s) 698
BshVI ATCGAT 1 cut(s) 607
BsiSI CCGG 1 cut(s) 681
BslI CCNNNNNNNGG 3 cut(s) 518, 686, 758
BsnI GGCC 2 cut(s) 365, 762
Bsp1407I TGTACA 1 cut(s) 715
Bsp143I GATC 5 cut(s) 127, 192, 421, 489, 604
BspACI CCGC 1 cut(s) 382
BspANI GGCC 2 cut(s) 365, 762
BspDI ATCGAT 1 cut(s) 607
BspLI GGNNCC 1 cut(s) 700
BspPI GGATC 1 cut(s) 497
BspT107I GGYRCC 1 cut(s) 698
BsrBI CCGCTC 1 cut(s) 384
BsrDI GCAATG 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 715
BssMI GATC 5 cut(s) 127, 192, 421, 489, 604
BssNAI GTATAC 1 cut(s) 727
Bst1107I GTATAC 1 cut(s) 727
Bst4CI ACNGT 4 cut(s) 47, 172, 218, 462
Bst6I CTCTTC 1 cut(s) 146
BstAUI TGTACA 1 cut(s) 715
BstBAI YACGTR 1 cut(s) 710
BstC8I GCNNGC 1 cut(s) 639
BstF5I GGATG 1 cut(s) 559
BstKTI GATC 5 cut(s) 130, 195, 424, 492, 607
BstMBI GATC 5 cut(s) 127, 192, 421, 489, 604
BstMWI GCNNNNNNNGC 1 cut(s) 752
BstNSI RCATGY 2 cut(s) 452, 641
BstSCI CCNGG 1 cut(s) 679
BstV1I GCAGC 3 cut(s) 125, 653, 656
BstZ17I GTATAC 1 cut(s) 727
Bsu15I ATCGAT 1 cut(s) 607
BsuI GTATCC 1 cut(s) 519
BsuRI GGCC 2 cut(s) 365, 762
BsuTUI ATCGAT 1 cut(s) 607
BtsCI GGATG 1 cut(s) 559
Cac8I GCNNGC 1 cut(s) 639
Cfr13I GGNCC 1 cut(s) 363
ClaI ATCGAT 1 cut(s) 607
Csp6I GTAC 4 cut(s) 625, 699, 716, 779
CviAII CATG 6 cut(s) 108, 133, 425, 449, 572, 638
CviJI RGCY 8 cut(s) 65, 185, 272, 312, 365, 381, 647, 762
CviKI_1 RGCY 8 cut(s) 65, 185, 272, 312, 365, 381, 647, 762
CviQI GTAC 4 cut(s) 625, 699, 716, 779
DpnI GATC 5 cut(s) 129, 194, 423, 491, 606
DpnII GATC 5 cut(s) 127, 192, 421, 489, 604
EaeI YGGCCR 1 cut(s) 760
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
FaeI CATG 6 cut(s) 111, 136, 428, 452, 575, 641
FatI CATG 6 cut(s) 107, 132, 424, 448, 571, 637
FbaI TGATCA 1 cut(s) 421
FblI GTMKAC 1 cut(s) 726
Fnu4HI GCNGC 4 cut(s) 114, 382, 642, 645
FokI GGATG 1 cut(s) 566
Fsp4HI GCNGC 4 cut(s) 114, 382, 642, 645
FspBI CTAG 2 cut(s) 186, 351
GluI GCNGC 4 cut(s) 114, 382, 642, 645
HaeIII GGCC 2 cut(s) 365, 762
HapII CCGG 1 cut(s) 681
Hin1II CATG 6 cut(s) 111, 136, 428, 452, 575, 641
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 3 cut(s) 282, 436, 658
HpaII CCGG 1 cut(s) 681
HphI GGTGA 1 cut(s) 741
Hpy166II GTNNAC 3 cut(s) 496, 727, 781
Hpy188I TCNGA 1 cut(s) 445
Hpy188III TCNNGA 3 cut(s) 34, 433, 592
Hpy8I GTNNAC 3 cut(s) 496, 727, 781
HpyAV CCTTC 1 cut(s) 544
HpyCH4III ACNGT 4 cut(s) 47, 172, 218, 462
HpyCH4IV ACGT 1 cut(s) 709
HpyCH4V TGCA 8 cut(s) 53, 113, 249, 302, 578, 637, 641, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 752
HpySE526I ACGT 1 cut(s) 709
Hsp92II CATG 6 cut(s) 111, 136, 428, 452, 575, 641
KpnI GGTACC 1 cut(s) 702
Ksp22I TGATCA 1 cut(s) 421
Kzo9I GATC 5 cut(s) 127, 192, 421, 489, 604
LpnPI CCDG 8 cut(s) 51, 282, 315, 378, 418, 694, 756, 765
Lsp1109I GCAGC 3 cut(s) 125, 653, 656
LweI GCATC 4 cut(s) 94, 225, 528, 755
MaeI CTAG 2 cut(s) 186, 351
MaeII ACGT 1 cut(s) 709
MaeIII GTNAC 2 cut(s) 34, 353
MalI GATC 5 cut(s) 129, 194, 423, 491, 606
MbiI CCGCTC 1 cut(s) 384
MboI GATC 5 cut(s) 127, 192, 421, 489, 604
MboII GAAGA 4 cut(s) 163, 331, 334, 614
MlsI TGGCCA 1 cut(s) 762
MluNI TGGCCA 1 cut(s) 762
MlyI GAGTC 1 cut(s) 430
MmeI TCCRAC 2 cut(s) 667, 817
MnlI CCTC 4 cut(s) 133, 480, 672, 769
Mox20I TGGCCA 1 cut(s) 762
MroXI GAANNNNTTC 1 cut(s) 261
MscI TGGCCA 1 cut(s) 762
MseI TTAA 5 cut(s) 77, 791, 809, 822, 842
MslI CAYNNNNRTG 2 cut(s) 90, 503
Msp20I TGGCCA 1 cut(s) 762
MspI CCGG 1 cut(s) 681
MspR9I CCNGG 1 cut(s) 681
MwoI GCNNNNNNNGC 1 cut(s) 752
NciI CCSGG 1 cut(s) 681
NdeII GATC 5 cut(s) 127, 192, 421, 489, 604
NlaIII CATG 6 cut(s) 111, 136, 428, 452, 575, 641
NlaIV GGNNCC 1 cut(s) 700
NmuCI GTSAC 2 cut(s) 34, 353
NspI RCATGY 2 cut(s) 452, 641
OliI CACNNNNGTG 1 cut(s) 503
PaeI GCATGC 1 cut(s) 641
PciI ACATGT 1 cut(s) 448
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 282, 658
PflMI CCANNNNNTGG 1 cut(s) 758
PkrI GCNGC 4 cut(s) 115, 383, 643, 646
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
Ppu21I YACGTR 1 cut(s) 710
PscI ACATGT 1 cut(s) 448
PshBI ATTAAT 1 cut(s) 822
PsiI TTATAA 2 cut(s) 798, 816
PspN4I GGNNCC 1 cut(s) 700
PspPI GGNCC 1 cut(s) 363
RsaI GTAC 4 cut(s) 626, 700, 717, 780
RsaNI GTAC 4 cut(s) 625, 699, 716, 779
RseI CAYNNNNRTG 2 cut(s) 90, 503
SaqAI TTAA 5 cut(s) 77, 791, 809, 822, 842
SatI GCNGC 4 cut(s) 114, 382, 642, 645
Sau3AI GATC 5 cut(s) 127, 192, 421, 489, 604
Sau96I GGNCC 1 cut(s) 363
SchI GAGTC 1 cut(s) 430
ScrFI CCNGG 1 cut(s) 681
SetI ASST 8 cut(s) 9, 67, 187, 274, 397, 472, 712, 780
SfaNI GCATC 4 cut(s) 94, 225, 528, 755
SmiMI CAYNNNNRTG 2 cut(s) 90, 503
SpeI ACTAGT 1 cut(s) 350
SphI GCATGC 1 cut(s) 641
SsiI CCGC 1 cut(s) 382
SspI AATATT 1 cut(s) 820
SspMI CTAG 2 cut(s) 186, 351
StyD4I CCNGG 1 cut(s) 679
TaaI ACNGT 4 cut(s) 47, 172, 218, 462
TaiI ACGT 1 cut(s) 712
TaqI TCGA 3 cut(s) 280, 607, 664
TaqII GACCGA 1 cut(s) 53
TatI WGTACW 1 cut(s) 715
TauI GCSGC 1 cut(s) 384
TfiI GAWTC 2 cut(s) 282, 658
Tru1I TTAA 5 cut(s) 77, 791, 809, 822, 842
Tru9I TTAA 5 cut(s) 77, 791, 809, 822, 842
TseFI GTSAC 2 cut(s) 34, 353
TseI GCWGC 3 cut(s) 113, 641, 644
Tsp45I GTSAC 2 cut(s) 34, 353
TspDTI ATGAA 2 cut(s) 95, 570
TspGWI ACGGA 1 cut(s) 502
Van91I CCANNNNNTGG 1 cut(s) 758
VspI ATTAAT 1 cut(s) 822
XapI RAATTY 1 cut(s) 242
XceI RCATGY 2 cut(s) 452, 641
XmiI GTMKAC 1 cut(s) 726
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 2 cut(s) 186, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.