MD15G1444800.v1.1

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000220
Physical Location & Seq
Forward (+)
54565787 .. 54567597
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1444800.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGAGGCGTCTAATTAGCAAGTTCTTTATCTATGAAAATGTCCCTGCTGTGAAGGCATCATCACATCATTTCAAAAATATGGTAGTGGGATGTCAACAGGCCGGTGTTGGAGTACAACCTCCCACTCCCTATGAGATAAGAAACAAATATTTGGATATGGAGTATAAAGACATTGGCGAGTATGTTAACAAGTTGAGGTCAAAGTGGGAAACTAATGGTTGCACAATCATGTGTGACGGATGGACCGGCCCGACCAGATTGTCTATCATCAACTTCATGGTATACTCCAAGGGAAAGACAATTTTTTTGAAGTCTGTTGATGCTTCAGACCATATAAAGAATTACAAGTATATTTACAAATTATTGAGGGATGTAATCATGGAGGTGAGAGAGTATAATGTTGTCCAAGTCGTGACCGACAACGGTTCTGCATTTGTCAAAGCTGGAAAAAAGTTAATGAAGCATCATAATGTGTTTTGGACATCATGTGCAGCACATTGTATTGATCTCATGTTTGAGGCAATGGGGAAGAGAGAGAATGTTGCTACTGTGGTCAAAAGAGCTAGAACGATCACAAATTATATTTACAATCACGGTTGGTTGTTGGCAAAGATGCGTGAATTTTACAAAGGAGAAATTATTCGTCCAGCTACCACTCGATTCGCCACCAACTATATTGCATTAGACAGCATACTCAAGAAGAAAGCAGGGTTGAAGCAACTATTCACTAGTGACGATTGGGCCAACCACAATTTGAGCCGCTCAAATACAGGTCGTATGGTGGAAAGTATAGTGCTTGATCATGCATTTTGGACTCAATCAGAACATGTGTGCCAAGTGTTTGAACCTCTTTACAAAGTTTTACGGATCGTTGACACAGAGGTGTATCCTACTATGGGGGCAGTATATGAGTTGATGCGTGTAGTGAAGGATGAATTGGAAAGAAAACATGGTGCAAGGTGGGTCATAAAGATAATTGAAGACCGATGGTATAAAACATTATACCACGATTTGCATGCAGCAGCTAACATGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

346

Amino Acids

40.15

Weight (kDa)

9.39

Isoelectric Point (pI)

26.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 41 - 191 3.9e-51 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 259
AccBSI CCGCTC 1 cut(s) 762
AccI GTMKAC 1 cut(s) 282
AciI CCGC 1 cut(s) 760
AclWI GGATC 1 cut(s) 875
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 1 cut(s) 309
AcyI GRCGYC 1 cut(s) 7
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 1 cut(s) 896
AflIII ACRYGT 1 cut(s) 826
AgsI TTSAA 5 cut(s) 73, 310, 715, 845, 980
AhlI ACTAGT 1 cut(s) 728
AjuI GAANNNNNNNTTGG 2 cut(s) 920, 952
AleI CACNNNNGTG 1 cut(s) 881
AluBI AGCT 4 cut(s) 443, 563, 650, 1025
AluI AGCT 4 cut(s) 443, 563, 650, 1025
AlwI GGATC 1 cut(s) 875
AoxI GGCC 3 cut(s) 99, 247, 741
ApeKI GCWGC 3 cut(s) 491, 1019, 1022
ApoI RAATTY 1 cut(s) 620
ArsI GACNNNNNNTTYG 2 cut(s) 289, 321
Asp700I GAANNNNTTC 1 cut(s) 639
AspS9I GGNCC 3 cut(s) 243, 248, 741
AsuHPI GGTGA 1 cut(s) 397
AvaII GGWCC 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 987
BbvI GCAGC 3 cut(s) 503, 1031, 1034
BccI CCATC 2 cut(s) 234, 981
BcgI CGANNNNNNTGC 2 cut(s) 998, 1032
BciVI GTATCC 1 cut(s) 897
BclI TGATCA 1 cut(s) 799
BcuI ACTAGT 1 cut(s) 728
BfaI CTAG 2 cut(s) 564, 729
BfuI GTATCC 1 cut(s) 897
BisI GCNGC 4 cut(s) 492, 760, 1020, 1023
BlsI GCNGC 4 cut(s) 493, 761, 1021, 1024
Bme18I GGWCC 1 cut(s) 243
BmgT120I GGNCC 3 cut(s) 243, 248, 741
BmsI GCATC 5 cut(s) 65, 310, 472, 603, 906
BpiI GAAGAC 1 cut(s) 987
BpuEI CTTGAG 1 cut(s) 680
BsaHI GRCGYC 1 cut(s) 7
BsaJI CCNNGG 1 cut(s) 288
Bsc4I CCNNNNNNNGG 1 cut(s) 896
Bse118I RCCGGY 2 cut(s) 101, 245
Bse3DI GCAATG 1 cut(s) 528
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 4 cut(s) 95, 245, 376, 937
BseLI CCNNNNNNNGG 1 cut(s) 896
BseMI GCAATG 1 cut(s) 528
BseXI GCAGC 3 cut(s) 503, 1031, 1034
BsgI GTGCAG 1 cut(s) 510
BshFI GGCC 3 cut(s) 101, 249, 743
BsiSI CCGG 2 cut(s) 102, 246
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 896
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 3 cut(s) 101, 249, 743
Bsp143I GATC 4 cut(s) 505, 570, 799, 867
BspACI CCGC 1 cut(s) 760
BspANI GGCC 3 cut(s) 101, 249, 743
BspPI GGATC 1 cut(s) 875
BsrBI CCGCTC 1 cut(s) 762
BsrDI GCAATG 1 cut(s) 528
BsrFI RCCGGY 2 cut(s) 101, 245
BssAI RCCGGY 2 cut(s) 101, 245
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 4 cut(s) 505, 570, 799, 867
BssNAI GTATAC 1 cut(s) 283
BssNI GRCGYC 1 cut(s) 7
BssT1I CCWWGG 1 cut(s) 288
Bst1107I GTATAC 1 cut(s) 283
Bst4CI ACNGT 3 cut(s) 425, 550, 596
Bst6I CTCTTC 1 cut(s) 524
BstACI GRCGYC 1 cut(s) 7
BstC8I GCNNGC 1 cut(s) 1017
BstF5I GGATG 4 cut(s) 95, 245, 376, 937
BstKTI GATC 4 cut(s) 508, 573, 802, 870
BstMBI GATC 4 cut(s) 505, 570, 799, 867
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNSI RCATGY 2 cut(s) 830, 1019
BstV1I GCAGC 3 cut(s) 503, 1031, 1034
BstV2I GAAGAC 1 cut(s) 987
BstZ17I GTATAC 1 cut(s) 283
BsuI GTATCC 1 cut(s) 897
BsuRI GGCC 3 cut(s) 101, 249, 743
BtsCI GGATG 4 cut(s) 95, 245, 376, 937
Cac8I GCNNGC 1 cut(s) 1017
Cfr10I RCCGGY 2 cut(s) 101, 245
Cfr13I GGNCC 3 cut(s) 243, 248, 741
Csp6I GTAC 1 cut(s) 113
CviJI RGCY 8 cut(s) 101, 249, 443, 563, 650, 743, 759, 1025
CviKI_1 RGCY 8 cut(s) 101, 249, 443, 563, 650, 743, 759, 1025
CviQI GTAC 1 cut(s) 113
DpnI GATC 4 cut(s) 507, 572, 801, 869
DpnII GATC 4 cut(s) 505, 570, 799, 867
DrdI GACNNNNNNGTC 1 cut(s) 259
DseDI GACNNNNNNGTC 1 cut(s) 259
Eam1104I CTCTTC 1 cut(s) 524
EarI CTCTTC 1 cut(s) 524
Eco130I CCWWGG 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 309
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 808
ErhI CCWWGG 1 cut(s) 288
FaqI GGGAC 1 cut(s) 26
FbaI TGATCA 1 cut(s) 799
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 4 cut(s) 492, 760, 1020, 1023
FokI GGATG 4 cut(s) 102, 252, 383, 944
Fsp4HI GCNGC 4 cut(s) 492, 760, 1020, 1023
FspBI CTAG 2 cut(s) 564, 729
GluI GCNGC 4 cut(s) 492, 760, 1020, 1023
HaeIII GGCC 3 cut(s) 101, 249, 743
HapII CCGG 2 cut(s) 102, 246
Hin1I GRCGYC 1 cut(s) 7
HincII GTYRAC 3 cut(s) 95, 187, 874
HindII GTYRAC 3 cut(s) 95, 187, 874
HinfI GANTC 2 cut(s) 660, 814
HpaI GTTAAC 1 cut(s) 187
HpaII CCGG 2 cut(s) 102, 246
HphI GGTGA 1 cut(s) 397
Hpy166II GTNNAC 4 cut(s) 95, 187, 283, 874
Hpy188I TCNGA 2 cut(s) 328, 823
Hpy188III TCNNGA 2 cut(s) 412, 697
Hpy8I GTNNAC 4 cut(s) 95, 187, 283, 874
HpyAV CCTTC 2 cut(s) 46, 922
HpyCH4III ACNGT 3 cut(s) 425, 550, 596
HpyCH4V TGCA 8 cut(s) 222, 431, 491, 680, 806, 956, 1015, 1019
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
Hsp92I GRCGYC 1 cut(s) 7
Ksp22I TGATCA 1 cut(s) 799
KspAI GTTAAC 1 cut(s) 187
Kzo9I GATC 4 cut(s) 505, 570, 799, 867
LpnPI CCDG 9 cut(s) 57, 83, 115, 259, 268, 429, 660, 693, 756
Lsp1109I GCAGC 3 cut(s) 503, 1031, 1034
LweI GCATC 5 cut(s) 65, 310, 472, 603, 906
MaeI CTAG 2 cut(s) 564, 729
MaeIII GTNAC 3 cut(s) 233, 412, 731
MalI GATC 4 cut(s) 507, 572, 801, 869
MbiI CCGCTC 1 cut(s) 762
MboI GATC 4 cut(s) 505, 570, 799, 867
MboII GAAGA 3 cut(s) 541, 712, 992
MlyI GAGTC 1 cut(s) 808
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 7 cut(s) 129, 189, 360, 376, 511, 858, 874
Mph1103I ATGCAT 1 cut(s) 808
MroXI GAANNNNTTC 1 cut(s) 639
MseI TTAA 3 cut(s) 186, 455, 1036
MslI CAYNNNNRTG 4 cut(s) 227, 383, 468, 881
MspI CCGG 2 cut(s) 102, 246
MwoI GCNNNNNNNGC 1 cut(s) 53
NdeII GATC 4 cut(s) 505, 570, 799, 867
NmuCI GTSAC 3 cut(s) 233, 412, 731
NsiI ATGCAT 1 cut(s) 808
NspI RCATGY 2 cut(s) 830, 1019
OliI CACNNNNGTG 1 cut(s) 881
PaeI GCATGC 1 cut(s) 1019
PciI ACATGT 1 cut(s) 826
PdmI GAANNNNTTC 1 cut(s) 639
PfeI GAWTC 1 cut(s) 660
PkrI GCNGC 4 cut(s) 493, 761, 1021, 1024
PleI GAGTC 1 cut(s) 808
PpsI GAGTC 1 cut(s) 808
PscI ACATGT 1 cut(s) 826
PspPI GGNCC 3 cut(s) 243, 248, 741
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
RseI CAYNNNNRTG 4 cut(s) 227, 383, 468, 881
SaqAI TTAA 3 cut(s) 186, 455, 1036
SatI GCNGC 4 cut(s) 492, 760, 1020, 1023
Sau3AI GATC 4 cut(s) 505, 570, 799, 867
Sau96I GGNCC 3 cut(s) 243, 248, 741
SchI GAGTC 1 cut(s) 808
SfaNI GCATC 5 cut(s) 65, 310, 472, 603, 906
SinI GGWCC 1 cut(s) 243
SmiMI CAYNNNNRTG 4 cut(s) 227, 383, 468, 881
SmlI CTYRAG 1 cut(s) 695
SmoI CTYRAG 1 cut(s) 695
SpeI ACTAGT 1 cut(s) 728
SphI GCATGC 1 cut(s) 1019
SsiI CCGC 1 cut(s) 760
SspI AATATT 1 cut(s) 149
SspMI CTAG 2 cut(s) 564, 729
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 3 cut(s) 425, 550, 596
TaqI TCGA 1 cut(s) 658
TaqII GACCGA 2 cut(s) 431, 999
TatI WGTACW 1 cut(s) 112
TauI GCSGC 1 cut(s) 762
TfiI GAWTC 1 cut(s) 660
Tru1I TTAA 3 cut(s) 186, 455, 1036
Tru9I TTAA 3 cut(s) 186, 455, 1036
TseFI GTSAC 3 cut(s) 233, 412, 731
TseI GCWGC 3 cut(s) 491, 1019, 1022
Tsp45I GTSAC 3 cut(s) 233, 412, 731
TspDTI ATGAA 4 cut(s) 48, 265, 473, 948
TspGWI ACGGA 2 cut(s) 252, 880
VpaK11BI GGWCC 1 cut(s) 243
XapI RAATTY 1 cut(s) 620
XceI RCATGY 2 cut(s) 830, 1019
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 639
XspI CTAG 2 cut(s) 564, 729
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.